CA2115342C - A method of detecting and discriminating between nucleic acid sequences - Google Patents

A method of detecting and discriminating between nucleic acid sequences Download PDF

Info

Publication number: CA2115342C
Authority: CA; Canada
Prior art keywords: nucleic acid; preselected; immobilized; acid sequence; sequence
Prior art date: 1992-06-17
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

CA002115342A

Other languages

English (en)

French (fr)

Other versions

CA2115342A1 (en

Inventor

Robert B. Wallace

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

City of Hope

Original Assignee

City of Hope

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1992-06-17

Filing date

1992-06-17

Publication date

2003-08-26

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25677006&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2115342(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

1992-06-17 Application filed by City of Hope filed Critical City of Hope

1992-06-17 Priority to CA002115342A priority Critical patent/CA2115342C/en

1992-06-17 Priority to EP92914420A priority patent/EP0607151B1/de

1992-06-17 Priority to PCT/US1992/005133 priority patent/WO1993025563A1/en

1992-06-17 Priority to JP6501425A priority patent/JPH06509946A/ja

1993-12-23 Publication of CA2115342A1 publication Critical patent/CA2115342A1/en

2003-08-26 Application granted granted Critical

2003-08-26 Publication of CA2115342C publication Critical patent/CA2115342C/en

2012-06-17 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

150000007523 nucleic acids Chemical group 0.000 title claims abstract description 130
238000000034 method Methods 0.000 title claims abstract description 43
108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 51
108020004707 nucleic acids Proteins 0.000 claims abstract description 44
102000039446 nucleic acids Human genes 0.000 claims abstract description 44
230000000295 complement effect Effects 0.000 claims abstract description 33
238000009396 hybridization Methods 0.000 claims abstract description 32
239000007787 solid Substances 0.000 claims abstract description 27
239000001226 triphosphate Substances 0.000 claims abstract description 18
235000011178 triphosphate Nutrition 0.000 claims abstract description 18
239000000203 mixture Substances 0.000 claims abstract description 12
UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims abstract description 5
108700028369 Alleles Proteins 0.000 claims description 53
239000002773 nucleotide Substances 0.000 claims description 51
125000003729 nucleotide group Chemical group 0.000 claims description 51
108091034117 Oligonucleotide Proteins 0.000 claims description 33
238000006243 chemical reaction Methods 0.000 claims description 30
230000003321 amplification Effects 0.000 claims description 13
238000003199 nucleic acid amplification method Methods 0.000 claims description 13
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FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 1
241001136792 Alle Species 0.000 description 1
USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
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KJLLKLRVCJAFRY-UHFFFAOYSA-N mebutizide Chemical class ClC1=C(S(N)(=O)=O)C=C2S(=O)(=O)NC(C(C)C(C)CC)NC2=C1 KJLLKLRVCJAFRY-UHFFFAOYSA-N 0.000 description 1
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125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification

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Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Genetics & Genomics (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Physics & Mathematics (AREA)
Immunology (AREA)
Microbiology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Chemical Kinetics & Catalysis (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)

CA002115342A 1992-06-17 1992-06-17 A method of detecting and discriminating between nucleic acid sequences Expired - Lifetime CA2115342C (en)

Priority Applications (4)

Application Number	Priority Date	Filing Date	Title
CA002115342A CA2115342C (en)	1992-06-17	1992-06-17	A method of detecting and discriminating between nucleic acid sequences
EP92914420A EP0607151B1 (de)	1992-06-17	1992-06-17	Verfahren zum nachweis und unterscheiden von nukleinsäuresequenzen
PCT/US1992/005133 WO1993025563A1 (en)	1992-06-17	1992-06-17	A method of detecting and discriminating between nucleic acid sequences
JP6501425A JPH06509946A (ja)	1992-06-17	1992-06-17	核酸を検出し識別する方法

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
CA002115342A CA2115342C (en)	1992-06-17	1992-06-17	A method of detecting and discriminating between nucleic acid sequences
PCT/US1992/005133 WO1993025563A1 (en)	1992-06-17	1992-06-17	A method of detecting and discriminating between nucleic acid sequences

Publications (2)

Publication Number	Publication Date
CA2115342A1 CA2115342A1 (en)	1993-12-23
CA2115342C true CA2115342C (en)	2003-08-26

Family

ID=25677006

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002115342A Expired - Lifetime CA2115342C (en)	1992-06-17	1992-06-17	A method of detecting and discriminating between nucleic acid sequences

Country Status (4)

Country	Link
EP (1)	EP0607151B1 (de)
JP (1)	JPH06509946A (de)
CA (1)	CA2115342C (de)
WO (1)	WO1993025563A1 (de)

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1992
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- 1992-06-17 CA CA002115342A patent/CA2115342C/en not_active Expired - Lifetime
- 1992-06-17 WO PCT/US1992/005133 patent/WO1993025563A1/en not_active Ceased
- 1992-06-17 JP JP6501425A patent/JPH06509946A/ja active Pending

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Publication number	Publication date
JPH06509946A (ja)	1994-11-10
CA2115342A1 (en)	1993-12-23
EP0607151A4 (de)	1996-11-27
EP0607151A1 (de)	1994-07-27
EP0607151B1 (de)	2002-11-13
WO1993025563A1 (en)	1993-12-23

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Date	Code	Title	Description
1997-06-11	EEER	Examination request
2012-06-17	MKEX	Expiry

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