CA2115342C - A method of detecting and discriminating between nucleic acid sequences - Google Patents

A method of detecting and discriminating between nucleic acid sequences Download PDF

Info

Publication number: CA2115342C
Authority: CA; Canada
Prior art keywords: nucleic acid; preselected; immobilized; acid sequence; sequence
Prior art date: 1992-06-17
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

CA002115342A

Other languages

English (en)

French (fr)

Other versions

CA2115342A1 (en

Inventor

Robert B. Wallace

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

City of Hope

Original Assignee

City of Hope

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1992-06-17

Filing date

1992-06-17

Publication date

2003-08-26

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25677006&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2115342(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

1992-06-17 Application filed by City of Hope filed Critical City of Hope

1992-06-17 Priority to PCT/US1992/005133 priority Critical patent/WO1993025563A1/en

1992-06-17 Priority to CA002115342A priority patent/CA2115342C/en

1992-06-17 Priority to JP6501425A priority patent/JPH06509946A/ja

1992-06-17 Priority to EP92914420A priority patent/EP0607151B1/de

1993-12-23 Publication of CA2115342A1 publication Critical patent/CA2115342A1/en

2003-08-26 Application granted granted Critical

2003-08-26 Publication of CA2115342C publication Critical patent/CA2115342C/en

2012-06-17 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

150000007523 nucleic acids Chemical group 0.000 title claims abstract description 130
238000000034 method Methods 0.000 title claims abstract description 43
108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 51
108020004707 nucleic acids Proteins 0.000 claims abstract description 44
102000039446 nucleic acids Human genes 0.000 claims abstract description 44
230000000295 complement effect Effects 0.000 claims abstract description 33
238000009396 hybridization Methods 0.000 claims abstract description 32
239000007787 solid Substances 0.000 claims abstract description 27
239000001226 triphosphate Substances 0.000 claims abstract description 18
235000011178 triphosphate Nutrition 0.000 claims abstract description 18
239000000203 mixture Substances 0.000 claims abstract description 12
UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims abstract description 5
108700028369 Alleles Proteins 0.000 claims description 53
239000002773 nucleotide Substances 0.000 claims description 51
125000003729 nucleotide group Chemical group 0.000 claims description 51
108091034117 Oligonucleotide Proteins 0.000 claims description 33
238000006243 chemical reaction Methods 0.000 claims description 30
230000003321 amplification Effects 0.000 claims description 13
238000003199 nucleic acid amplification method Methods 0.000 claims description 13
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FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 1
241001136792 Alle Species 0.000 description 1
USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
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KJLLKLRVCJAFRY-UHFFFAOYSA-N mebutizide Chemical class ClC1=C(S(N)(=O)=O)C=C2S(=O)(=O)NC(C(C)C(C)CC)NC2=C1 KJLLKLRVCJAFRY-UHFFFAOYSA-N 0.000 description 1
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125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification

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Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Genetics & Genomics (AREA)
Microbiology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Physics & Mathematics (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Immunology (AREA)
Chemical Kinetics & Catalysis (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)

CA002115342A 1992-06-17 1992-06-17 A method of detecting and discriminating between nucleic acid sequences Expired - Lifetime CA2115342C (en)

Priority Applications (4)

Application Number	Priority Date	Filing Date	Title
PCT/US1992/005133 WO1993025563A1 (en)	1992-06-17	1992-06-17	A method of detecting and discriminating between nucleic acid sequences
CA002115342A CA2115342C (en)	1992-06-17	1992-06-17	A method of detecting and discriminating between nucleic acid sequences
JP6501425A JPH06509946A (ja)	1992-06-17	1992-06-17	核酸を検出し識別する方法
EP92914420A EP0607151B1 (de)	1992-06-17	1992-06-17	Verfahren zum nachweis und unterscheiden von nukleinsäuresequenzen

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
PCT/US1992/005133 WO1993025563A1 (en)	1992-06-17	1992-06-17	A method of detecting and discriminating between nucleic acid sequences
CA002115342A CA2115342C (en)	1992-06-17	1992-06-17	A method of detecting and discriminating between nucleic acid sequences

Publications (2)

Publication Number	Publication Date
CA2115342A1 CA2115342A1 (en)	1993-12-23
CA2115342C true CA2115342C (en)	2003-08-26

Family

ID=25677006

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002115342A Expired - Lifetime CA2115342C (en)	1992-06-17	1992-06-17	A method of detecting and discriminating between nucleic acid sequences

Country Status (4)

Country	Link
EP (1)	EP0607151B1 (de)
JP (1)	JPH06509946A (de)
CA (1)	CA2115342C (de)
WO (1)	WO1993025563A1 (de)

Families Citing this family (82)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5547839A (en)	1989-06-07	1996-08-20	Affymax Technologies N.V.	Sequencing of surface immobilized polymers utilizing microflourescence detection
US5710028A (en) *	1992-07-02	1998-01-20	Eyal; Nurit	Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
US6180403B1 (en) *	1999-10-28	2001-01-30	Isis Pharmaceuticals Inc.	Antisense inhibition of tumor necrosis factor alpha converting enzyme (TACE) expression
JP3419850B2 (ja) *	1993-10-26	2003-06-23	株式会社日立製作所	核酸分別方法
GB9415129D0 (en) *	1994-07-27	1994-09-14	Lynxvale Ltd	Oligonucleotides and their use
JP3612092B2 (ja) *	1994-09-07	2005-01-19	株式会社日立製作所	Ｄｎａの分離・分取法及びその解析法
GB9507238D0 (en) *	1995-04-07	1995-05-31	Isis Innovation	Detecting dna sequence variations
WO1997010366A2 (en) *	1995-09-15	1997-03-20	Genzyme Corporation	High throughput screening method for sequences or genetic alterations in nucleic acids
US6852487B1 (en)	1996-02-09	2005-02-08	Cornell Research Foundation, Inc.	Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
CA2255599C (en)	1996-04-25	2006-09-05	Bioarray Solutions, Llc	Light-controlled electrokinetic assembly of particles near surfaces
GB2312747B (en) *	1996-05-04	1998-07-22	Zeneca Ltd	Method for the detection of diagnostic base sequences using tailed primers having a detector region
GB9609441D0 (en)	1996-05-04	1996-07-10	Zeneca Ltd	Process
EP2369007B1 (de)	1996-05-29	2015-07-29	Cornell Research Foundation, Inc.	Erkennung von Nukleinsäuresequenz-Unterschieden mittels der gleichzeitigen Ligaseerkennungs- und Polymerasekettenreaktion
CA2276462C (en)	1996-12-31	2007-06-12	High Throughput Genomics, Inc.	Multiplexed molecular analysis system apparatus and method
US7622294B2 (en)	1997-03-14	2009-11-24	Trustees Of Tufts College	Methods for detecting target analytes and enzymatic reactions
US6327410B1 (en)	1997-03-14	2001-12-04	The Trustees Of Tufts College	Target analyte sensors utilizing Microspheres
US20030027126A1 (en)	1997-03-14	2003-02-06	Walt David R.	Methods for detecting target analytes and enzymatic reactions
US7115884B1 (en)	1997-10-06	2006-10-03	Trustees Of Tufts College	Self-encoding fiber optic sensor
US7348181B2 (en)	1997-10-06	2008-03-25	Trustees Of Tufts College	Self-encoding sensor with microspheres
US20030096232A1 (en)	1997-12-19	2003-05-22	Kris Richard M.	High throughput assay system
US6458533B1 (en) *	1997-12-19	2002-10-01	High Throughput Genomics, Inc.	High throughput assay system for monitoring ESTs
EP1090293B2 (de)	1998-06-24	2019-01-23	Illumina, Inc.	Dekodierung von matrixartig-angeordneten Sensoren durch Mikropartikel
US6451525B1 (en)	1998-12-03	2002-09-17	Pe Corporation (Ny)	Parallel sequencing method
GB9902971D0 (en) *	1999-02-11	1999-03-31	Zeneca Ltd	Assay
GB9902970D0 (en) *	1999-02-11	1999-03-31	Zeneca Ltd	Novel matrix
AU775179B2 (en) *	1999-03-15	2004-07-22	Applied Biosystems, Llc	Probe/mobility modifier complexes for multiplex nucleic acid detection
US7014994B1 (en)	1999-03-19	2006-03-21	Cornell Research Foundation,Inc.	Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process
ATE342378T1 (de) *	1999-03-19	2006-11-15	Cornell Res Foundation Inc	Verfahren das auf den gekoppelten reactionen der polymerase-kettenreaktion (pcr)-verdauung mit restriktionsenzymen und ligasenachweis basiert
JP2002539849A (ja) *	1999-03-26	2002-11-26	ホワイトヘッドインスチチュートフォアーバイオメディカルリサーチ	ユニバーサルアレイ
EP1183387A2 (de) *	1999-04-09	2002-03-06	Arcturus Engineering, Inc.	Generischer cdna oder proteinmatrix für angepasste tests
EP1173612B1 (de) *	1999-04-09	2004-09-29	Keygene N.V.	Verfahren zur erkennung und/oder analyse, unter verwendung von primertechniken, von einfachen nukleotidpolymorphismen in restriktionsfragmenten speziell aus amplifizierten restriktionsfragmenten generiert durch aflp
US6573047B1 (en)	1999-04-13	2003-06-03	Dna Sciences, Inc.	Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation
US6355431B1 (en)	1999-04-20	2002-03-12	Illumina, Inc.	Detection of nucleic acid amplification reactions using bead arrays
US20060275782A1 (en)	1999-04-20	2006-12-07	Illumina, Inc.	Detection of nucleic acid reactions on bead arrays
WO2000065098A2 (en) *	1999-04-27	2000-11-02	University Of Chicago	Nucleotide extension on a microarray of gel-immobilized primers
US6544732B1 (en)	1999-05-20	2003-04-08	Illumina, Inc.	Encoding and decoding of array sensors utilizing nanocrystals
EP1190100B1 (de)	1999-05-20	2012-07-25	Illumina, Inc.	Kombinatorische entschlüsselung von zufallsnukleinsäurearrays
US8481268B2 (en)	1999-05-21	2013-07-09	Illumina, Inc.	Use of microfluidic systems in the detection of target analytes using microsphere arrays
US8080380B2 (en)	1999-05-21	2011-12-20	Illumina, Inc.	Use of microfluidic systems in the detection of target analytes using microsphere arrays
US6642001B1 (en)	1999-07-13	2003-11-04	Whitehead Institute For Biomedical Research	Generic SBE-FRET protocol
EP1196623B8 (de) *	1999-07-23	2008-05-21	Forensic Science Service Ltd	Verfahren zur Erkennung von Einzel-Nukleotid-Polymorphismen
US6316230B1 (en)	1999-08-13	2001-11-13	Applera Corporation	Polymerase extension at 3′ terminus of PNA-DNA chimera
CA2382157C (en)	1999-08-18	2012-04-03	Illumina, Inc.	Compositions and methods for preparing oligonucleotide solutions
JP2003510054A (ja) *	1999-09-22	2003-03-18	モトローラ・インコーポレイテッド	単一ヌクレオチド多形性の平行遺伝子型決定のための三次元ミクロアレイシステム
US6709816B1 (en) *	1999-10-18	2004-03-23	Affymetrix, Inc.	Identification of alleles
US6287778B1 (en) *	1999-10-19	2001-09-11	Affymetrix, Inc.	Allele detection using primer extension with sequence-coded identity tags
WO2001036669A2 (de) *	1999-11-12	2001-05-25	Epigenomics Ag	Verfahren zur kontrollierbaren durchführung komplexer pcr-amplifikationen
DE19957827C2 (de)	1999-11-25	2003-06-12	Epigenomics Ag	Verwendung eines Oligomer-Arrays mit PNA- und/oder DNA-Oligomeren auf einer Oberfläche
IL149676A0 (en)	1999-12-02	2002-11-10	Dna Sciences Inc	Methods and kits for determining nucleotide variations
JP2003522527A (ja) *	1999-12-28	2003-07-29	グラクソグループリミテッド	一塩基多型の検出
DK1259643T3 (da)	2000-02-07	2009-02-23	Illumina Inc	Fremgangsmåde til detektion af nukleinsyre ved anvendelse af universel priming
US7611869B2 (en)	2000-02-07	2009-11-03	Illumina, Inc.	Multiplexed methylation detection methods
WO2001057268A2 (en) *	2000-02-07	2001-08-09	Illumina, Inc.	Nucleic acid detection methods using universal priming
US7582420B2 (en)	2001-07-12	2009-09-01	Illumina, Inc.	Multiplex nucleic acid reactions
US7361488B2 (en)	2000-02-07	2008-04-22	Illumina, Inc.	Nucleic acid detection methods using universal priming
US6913884B2 (en)	2001-08-16	2005-07-05	Illumina, Inc.	Compositions and methods for repetitive use of genomic DNA
US6770441B2 (en)	2000-02-10	2004-08-03	Illumina, Inc.	Array compositions and methods of making same
ATE412774T1 (de)	2000-02-16	2008-11-15	Illumina Inc	Parallele genotypisierung mehrerer patientenproben
WO2001079548A2 (en)	2000-04-14	2001-10-25	Cornell Research Foundation, Inc.	Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
GB0009294D0 (en)	2000-04-15	2000-05-31	Sec Dep For The Home Departmen	Improvements in and relating to analysis of DNA samples
AU2001265121A1 (en) *	2000-05-30	2001-12-11	Applera Corporation	Methods for detecting target nucleic acids using coupled ligation and amplification
US6355433B1 (en)	2000-06-02	2002-03-12	Dna Sciences, Inc.	Determination of nucleotide sequence variations through limited primer extension
EP1164201A1 (de) *	2000-06-14	2001-12-19	Facultés Universitaires Notre-Dame de la Paix	Umgekehrter nachweis zur Identifizierung und/oder Quantifizierung von Nukleotid-Zielsequenzen mittels Biochips
US9709559B2 (en)	2000-06-21	2017-07-18	Bioarray Solutions, Ltd.	Multianalyte molecular analysis using application-specific random particle arrays
WO2002018659A2 (en) *	2000-08-30	2002-03-07	Haplogen, Llc	Method for determining alleles
DE60131903T2 (de)	2000-10-24	2008-11-27	The Board of Trustees of the Leland S. Stanford Junior University, Palo Alto	Direkte multiplex charakterisierung von genomischer dna
AUPR221400A0 (en)	2000-12-20	2001-01-25	Murdoch Childrens Research Institute, The	Diagnostic assay
US20030165865A1 (en) *	2001-01-29	2003-09-04	Hinkel Christopher A.	Methods of analysis of nucleic acids
US7262063B2 (en)	2001-06-21	2007-08-28	Bio Array Solutions, Ltd.	Directed assembly of functional heterostructures
US6893822B2 (en)	2001-07-19	2005-05-17	Nanogen Recognomics Gmbh	Enzymatic modification of a nucleic acid-synthetic binding unit conjugate
CA2497740C (en)	2001-10-15	2011-06-21	Bioarray Solutions, Ltd.	Multiplexed analysis of polymorphic loci by probe elongation-mediated detection
AU2003215240A1 (en)	2002-02-14	2003-09-04	Illumina, Inc.	Automated information processing in randomly ordered arrays
US7238476B2 (en)	2002-08-02	2007-07-03	Orchid Cellmark, Inc.	Methods and compositions for genotyping
US7192700B2 (en)	2002-12-20	2007-03-20	Orchid Cellmark Inc.	Methods and compositions for conducting primer extension and polymorphism detection reactions
AU2003298655A1 (en)	2002-11-15	2004-06-15	Bioarray Solutions, Ltd.	Analysis, secure access to, and transmission of array images
WO2004065000A1 (en)	2003-01-21	2004-08-05	Illumina Inc.	Chemical reaction monitor
CA2539824C (en)	2003-09-22	2015-02-03	Xinwen Wang	Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules
CA2544041C (en)	2003-10-28	2015-12-08	Bioarray Solutions Ltd.	Optimization of gene expression analysis using immobilized capture probes
US7338763B2 (en)	2004-06-02	2008-03-04	Eppendorf Array Technologies S.A.	Method and kit for the detection and/or quantification of homologous nucleotide sequences on arrays
US7848889B2 (en)	2004-08-02	2010-12-07	Bioarray Solutions, Ltd.	Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
US9109256B2 (en)	2004-10-27	2015-08-18	Esoterix Genetic Laboratories, Llc	Method for monitoring disease progression or recurrence
US9777314B2 (en)	2005-04-21	2017-10-03	Esoterix Genetic Laboratories, Llc	Analysis of heterogeneous nucleic acid samples

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU3694689A (en) *	1988-04-28	1989-11-24	Mark H. Skolnick	Amplified sequence polymorphisms (asps)
IE66572B1 (en) *	1989-02-13	1996-01-24	Geneco Pty Ltd	Detection of a nucleic acid sequence or a change therein
AU5640090A (en) *	1989-03-21	1990-11-05	Collaborative Research Inc.	A dna diagnostic test using an exonuclease activity
GB8920097D0 (en) *	1989-09-06	1989-10-18	Ici Plc	Amplification processes
IL97222A (en) *	1990-02-16	1995-08-31	Orion Yhtymae Oy	Method and reagent for determining specific nucleotide variations

1992
- 1992-06-17 CA CA002115342A patent/CA2115342C/en not_active Expired - Lifetime
- 1992-06-17 JP JP6501425A patent/JPH06509946A/ja active Pending
- 1992-06-17 EP EP92914420A patent/EP0607151B1/de not_active Revoked
- 1992-06-17 WO PCT/US1992/005133 patent/WO1993025563A1/en not_active Ceased

Also Published As

Publication number	Publication date
EP0607151B1 (de)	2002-11-13
CA2115342A1 (en)	1993-12-23
JPH06509946A (ja)	1994-11-10
EP0607151A1 (de)	1994-07-27
WO1993025563A1 (en)	1993-12-23
EP0607151A4 (de)	1996-11-27

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1997-06-11	EEER	Examination request
2012-06-17	MKEX	Expiry

Publication	Publication Date	Title
CA2115342C (en)	2003-08-26	A method of detecting and discriminating between nucleic acid sequences
US5981176A (en)	1999-11-09	Method of detecting and discriminating between nucleic acid sequences
CA1340121E (en)	1998-11-10	Process for amplifying, detecting and/or cloning nucleic acid sequences
AU704625B2 (en)	1999-04-29	Method for characterizing nucleic acid molecules
US4683194A (en)	1987-07-28	Method for detection of polymorphic restriction sites and nucleic acid sequences
AU635212B2 (en)	1993-03-18	A method for the amplification of nucleotide sequences
US4683195A (en)	1987-07-28	Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5468613A (en)	1995-11-21	Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
CA2071537C (en)	2003-02-11	Method and reagent for determining specific nucleotide variations
US5658764A (en)	1997-08-19	Method and kits for detection of fragile X specific, GC-rich DNA sequences
WO1990011372A1 (en)	1990-10-04	Multiplex dna diagnostic test
JPH10509594A (ja)	1998-09-22	遺伝的多型の検出のための複合ミクロサテライトプライマー
KR20110014997A (ko)	2011-02-14	Ｄｎａ 기재의 프로파일링 검정을 위한 물질 및 방법
WO1994021820A1 (en)	1994-09-29	Nucleic acid sequences having single stranded ends
EP0402400B1 (de)	1999-09-08	Verwendung von dna-proben mit variabler anzahl von tandem-repetitiven stellen zur genetischen identifizierung
Ugozzoli et al.	1992	Detection of specific alleles by using allele-specific primer extension followed by capture on solid support
CA2089351A1 (en)	1993-08-25	Gaucher's disease: detection of a new mutation in intron 2 of the glucocerebrosidase gene
WU et al.	1989	Direct analysis of single nucleotide variation in human DNA and RNA using in situ dot hybridization
KR101249635B1 (ko)	2013-04-01	양극성 장애 진단용 ｅｇｒ2 유전자 ｓｎｐ, 그를 포함하는 마이크로어레이 및 키트
CA2455354A1 (en)	2002-09-26	Method of constructing cdna tag for identifying expressed gene and method of analyzing gene expression
EP1302547A2 (de)	2003-04-16	Verfahren zum Nachweis und Unterscheiden von Nukleinsäuresequenzen
AU674127C (en)	1998-05-28	A method of detecting and discriminating between nucleic acid sequences
AU2005266805B2 (en)	2010-10-28	Method of detecting mutations in the gene encoding Cytochrome P450-2C19
WO2002079518A1 (en)	2002-10-10	A method for genotyping individuals for multiple snps
Randall	1990	A novel in vitro assay to study the mechanism by which DNA polymerases bypass blocking lesions to DNA replication.