CA2148140C - Homogeneous method for assay of double-stranded nucleic acids using fluorescent dyes and kit useful therein - Google Patents

Homogeneous method for assay of double-stranded nucleic acids using fluorescent dyes and kit useful therein Download PDF

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Publication number: CA2148140C
Authority: CA; Canada
Prior art keywords: carbon atoms; nucleic acid; double; target nucleic; alkyl
Prior art date: 1994-04-29
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

CA2148140A

Other languages

English (en)

French (fr)

Other versions

CA2148140A1 (en

Inventor

John W. Sutherland

David R. Patterson

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Ortho Clinical Diagnostics Inc

Original Assignee

Ortho Clinical Diagnostics Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1994-04-29

Filing date

1995-04-28

Publication date

2010-08-10

1995-04-28 Application filed by Ortho Clinical Diagnostics Inc filed Critical Ortho Clinical Diagnostics Inc

1995-10-30 Publication of CA2148140A1 publication Critical patent/CA2148140A1/en

2010-08-10 Application granted granted Critical

2010-08-10 Publication of CA2148140C publication Critical patent/CA2148140C/en

2015-04-28 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

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150000007523 nucleic acids Chemical class 0.000 title claims abstract description 92
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238000000034 method Methods 0.000 title claims description 60
238000003556 assay Methods 0.000 title abstract description 18
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125000000217 alkyl group Chemical group 0.000 claims description 28
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit

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Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Investigating Or Analysing Biological Materials (AREA)
Plural Heterocyclic Compounds (AREA)
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CA2148140A 1994-04-29 1995-04-28 Homogeneous method for assay of double-stranded nucleic acids using fluorescent dyes and kit useful therein Expired - Lifetime CA2148140C (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US23539694A	1994-04-29	1994-04-29
US08/235,396		1994-04-29

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CA2148140A1 CA2148140A1 (en)	1995-10-30
CA2148140C true CA2148140C (en)	2010-08-10

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CA2148140A Expired - Lifetime CA2148140C (en)	1994-04-29	1995-04-28	Homogeneous method for assay of double-stranded nucleic acids using fluorescent dyes and kit useful therein

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US (1)	US5563037A (de)
EP (1)	EP0684316B1 (de)
JP (1)	JP4260903B2 (de)
KR (1)	KR100388732B1 (de)
CN (1)	CN1100884C (de)
AT (1)	ATE280243T1 (de)
CA (1)	CA2148140C (de)
DE (1)	DE69533660T2 (de)

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1995
- 1995-04-28 EP EP95302921A patent/EP0684316B1/de not_active Expired - Lifetime
- 1995-04-28 AT AT95302921T patent/ATE280243T1/de not_active IP Right Cessation
- 1995-04-28 CA CA2148140A patent/CA2148140C/en not_active Expired - Lifetime
- 1995-04-28 DE DE69533660T patent/DE69533660T2/de not_active Expired - Lifetime
- 1995-04-29 KR KR1019950010417A patent/KR100388732B1/ko not_active Expired - Fee Related
- 1995-04-29 CN CN95106515A patent/CN1100884C/zh not_active Expired - Lifetime
- 1995-05-01 JP JP10746895A patent/JP4260903B2/ja not_active Expired - Fee Related
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Publication number	Publication date
EP0684316A1 (de)	1995-11-29
DE69533660D1 (de)	2004-11-25
KR950032641A (ko)	1995-12-22
KR100388732B1 (ko)	2004-09-04
EP0684316B1 (de)	2004-10-20
CN1114359A (zh)	1996-01-03
JPH0889299A (ja)	1996-04-09
ATE280243T1 (de)	2004-11-15
CN1100884C (zh)	2003-02-05
CA2148140A1 (en)	1995-10-30
DE69533660T2 (de)	2005-11-10
JP4260903B2 (ja)	2009-04-30
US5563037A (en)	1996-10-08

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EP0684316B1 (de)	2004-10-20	Homogenes Verfahren zum Nachweis von Doppelstrang-Nukleinsäuren mittels fluoreszierender Farbstoffe und dafür nützliche Kits
EP0702090B1 (de)	2004-05-06	Verfahren zur Koamplifikation einer Zielnukleinsäure unter Versendung eines Volumenabscheidungsmittel in der Reaktionszusammensetzung, Testsatz und Testvorrichtung
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EP0677589B1 (de)	2001-05-16	Verfahren, Testelement und Testsatz zum halbquantitativen Nachweis von Nukleinsäure
CA2024978A1 (en)	1991-03-23	Nucleic acid detection method using unequal primer concentrations in polymerase chain reaction
JPH07298897A (ja)	1995-11-14	２つの標識プライマーを用いた標的核酸の生成、増幅及び検出方法並びにそれらを含有するｐｃｒキット
HK1004861B (en)	2001-10-26	Nucleic acid amplification and detection methods using rapid polymerase chain reaction cycle