CA2149784A1 - Detection de ligands par modulation d'interference - Google Patents

Detection de ligands par modulation d'interference

Info

Publication number: CA2149784A1
Authority: CA; Canada
Prior art keywords: assay; ligand; change; slits; interference pattern
Prior art date: 1992-11-24
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA002149784A

Other languages

English (en)

Inventor

Keith C. Backman

Christiane Munkholm

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Omnigene Inc

Original Assignee

Individual

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1992-11-24

Filing date

1992-11-24

Publication date

1994-06-09

1992-11-24 Application filed by Individual filed Critical Individual

1992-11-24 Priority to CA002149784A priority Critical patent/CA2149784A1/fr

1994-06-09 Publication of CA2149784A1 publication Critical patent/CA2149784A1/fr

Status Abandoned legal-status Critical Current

Links

238000003556 assay Methods 0.000 title claims abstract description 92
239000003446 ligand Substances 0.000 title claims abstract description 70
230000003287 optical effect Effects 0.000 claims abstract description 39
238000001514 detection method Methods 0.000 claims abstract description 18
230000001419 dependent effect Effects 0.000 claims abstract description 4
230000008859 change Effects 0.000 claims description 55
238000000034 method Methods 0.000 claims description 48
238000002835 absorbance Methods 0.000 claims description 17
239000000758 substrate Substances 0.000 claims description 9
230000004044 response Effects 0.000 claims description 5
239000002245 particle Substances 0.000 claims description 4
230000010287 polarization Effects 0.000 claims description 4
241000894006 Bacteria Species 0.000 claims description 2
239000002184 metal Substances 0.000 claims description 2
229910052751 metal Inorganic materials 0.000 claims description 2
230000005693 optoelectronics Effects 0.000 claims 1
238000003018 immunoassay Methods 0.000 abstract description 11
230000005540 biological transmission Effects 0.000 description 25
230000006870 function Effects 0.000 description 22
239000000427 antigen Substances 0.000 description 17
102000036639 antigens Human genes 0.000 description 16
108091007433 antigens Proteins 0.000 description 16
238000009739 binding Methods 0.000 description 16
PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 16
239000012491 analyte Substances 0.000 description 14
238000006243 chemical reaction Methods 0.000 description 14
230000005855 radiation Effects 0.000 description 14
230000027455 binding Effects 0.000 description 13
230000035945 sensitivity Effects 0.000 description 11
BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 10
229910052709 silver Inorganic materials 0.000 description 10
239000004332 silver Substances 0.000 description 10
239000011521 glass Substances 0.000 description 8
239000003153 chemical reaction reagent Substances 0.000 description 7
239000011888 foil Substances 0.000 description 7
239000000463 material Substances 0.000 description 7
238000005259 measurement Methods 0.000 description 7
238000012360 testing method Methods 0.000 description 7
102000004190 Enzymes Human genes 0.000 description 6
108090000790 Enzymes Proteins 0.000 description 6
YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 6
238000011534 incubation Methods 0.000 description 6
230000007423 decrease Effects 0.000 description 5
229940079593 drug Drugs 0.000 description 5
239000003814 drug Substances 0.000 description 5
150000002500 ions Chemical class 0.000 description 5
238000000926 separation method Methods 0.000 description 5
241000283973 Oryctolagus cuniculus Species 0.000 description 4
JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 4
238000013459 approach Methods 0.000 description 4
230000015572 biosynthetic process Effects 0.000 description 4
238000010586 diagram Methods 0.000 description 4
239000010931 gold Substances 0.000 description 4
229910052737 gold Inorganic materials 0.000 description 4
238000001556 precipitation Methods 0.000 description 4
239000007787 solid Substances 0.000 description 4
241000894007 species Species 0.000 description 4
BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 3
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
239000000872 buffer Substances 0.000 description 3
150000001718 carbodiimides Chemical class 0.000 description 3
150000001875 compounds Chemical class 0.000 description 3
230000000670 limiting effect Effects 0.000 description 3
239000008363 phosphate buffer Substances 0.000 description 3
230000008569 process Effects 0.000 description 3
102000004169 proteins and genes Human genes 0.000 description 3
108090000623 proteins and genes Proteins 0.000 description 3
101710125089 Bindin Proteins 0.000 description 2
241000283707 Capra Species 0.000 description 2
RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
238000004458 analytical method Methods 0.000 description 2
230000008901 benefit Effects 0.000 description 2
238000005119 centrifugation Methods 0.000 description 2
230000002860 competitive effect Effects 0.000 description 2
230000002596 correlated effect Effects 0.000 description 2
230000003247 decreasing effect Effects 0.000 description 2
238000000151 deposition Methods 0.000 description 2
230000008021 deposition Effects 0.000 description 2
238000005516 engineering process Methods 0.000 description 2
238000002474 experimental method Methods 0.000 description 2
MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
230000007935 neutral effect Effects 0.000 description 2
229920000642 polymer Polymers 0.000 description 2
UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 2
-1 p~astic Substances 0.000 description 2
238000003127 radioimmunoassay Methods 0.000 description 2
230000003595 spectral effect Effects 0.000 description 2
238000004611 spectroscopical analysis Methods 0.000 description 2
230000026683 transduction Effects 0.000 description 2
238000010361 transduction Methods 0.000 description 2
238000005406 washing Methods 0.000 description 2
241000182988 Assa Species 0.000 description 1
241001436672 Bhatia Species 0.000 description 1
BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
241000905957 Channa melasoma Species 0.000 description 1
229920000858 Cyclodextrin Polymers 0.000 description 1
VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
239000005977 Ethylene Substances 0.000 description 1
101001091385 Homo sapiens Kallikrein-6 Proteins 0.000 description 1
238000004566 IR spectroscopy Methods 0.000 description 1
235000000177 Indigofera tinctoria Nutrition 0.000 description 1
102100034866 Kallikrein-6 Human genes 0.000 description 1
206010028980 Neoplasm Diseases 0.000 description 1
238000001069 Raman spectroscopy Methods 0.000 description 1
240000004543 Vicia ervilia Species 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
238000000862 absorption spectrum Methods 0.000 description 1
239000002253 acid Substances 0.000 description 1
230000004913 activation Effects 0.000 description 1
230000001919 adrenal effect Effects 0.000 description 1
150000003973 alkyl amines Chemical group 0.000 description 1
230000004075 alteration Effects 0.000 description 1
150000001408 amides Chemical class 0.000 description 1
150000001412 amines Chemical class 0.000 description 1
238000003149 assay kit Methods 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
239000011324 bead Substances 0.000 description 1
235000013405 beer Nutrition 0.000 description 1
230000008033 biological extinction Effects 0.000 description 1
230000000903 blocking effect Effects 0.000 description 1
239000008280 blood Substances 0.000 description 1
210000004369 blood Anatomy 0.000 description 1
238000004364 calculation method Methods 0.000 description 1
125000002843 carboxylic acid group Chemical group 0.000 description 1
230000002925 chemical effect Effects 0.000 description 1
238000000978 circular dichroism spectroscopy Methods 0.000 description 1
230000001427 coherent effect Effects 0.000 description 1
239000000084 colloidal system Substances 0.000 description 1
239000003086 colorant Substances 0.000 description 1
239000000356 contaminant Substances 0.000 description 1
238000011109 contamination Methods 0.000 description 1
LBJNMUFDOHXDFG-UHFFFAOYSA-N copper;hydrate Chemical compound O.[Cu].[Cu] LBJNMUFDOHXDFG-UHFFFAOYSA-N 0.000 description 1
230000000875 corresponding effect Effects 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
239000013078 crystal Substances 0.000 description 1
229940097362 cyclodextrins Drugs 0.000 description 1
238000007405 data analysis Methods 0.000 description 1
238000011161 development Methods 0.000 description 1
230000018109 developmental process Effects 0.000 description 1
239000006185 dispersion Substances 0.000 description 1
238000009826 distribution Methods 0.000 description 1
230000000694 effects Effects 0.000 description 1
230000007613 environmental effect Effects 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
230000005284 excitation Effects 0.000 description 1
238000013401 experimental design Methods 0.000 description 1
239000012530 fluid Substances 0.000 description 1
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
238000000198 fluorescence anisotropy Methods 0.000 description 1
238000002875 fluorescence polarization Methods 0.000 description 1
238000001506 fluorescence spectroscopy Methods 0.000 description 1
239000007850 fluorescent dye Substances 0.000 description 1
239000005556 hormone Substances 0.000 description 1
229940088597 hormone Drugs 0.000 description 1
230000003100 immobilizing effect Effects 0.000 description 1
229940127121 immunoconjugate Drugs 0.000 description 1
229940097275 indigo Drugs 0.000 description 1
COHYTHOBJLSHDF-UHFFFAOYSA-N indigo powder Natural products N1C2=CC=CC=C2C(=O)C1=C1C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-UHFFFAOYSA-N 0.000 description 1
239000012678 infectious agent Substances 0.000 description 1
238000003780 insertion Methods 0.000 description 1
230000037431 insertion Effects 0.000 description 1
230000003993 interaction Effects 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
230000000155 isotopic effect Effects 0.000 description 1
IYRMWMYZSQPJKC-UHFFFAOYSA-N kaempferol Chemical compound C1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 IYRMWMYZSQPJKC-UHFFFAOYSA-N 0.000 description 1
230000004807 localization Effects 0.000 description 1
239000011159 matrix material Substances 0.000 description 1
230000007246 mechanism Effects 0.000 description 1
239000012528 membrane Substances 0.000 description 1
239000011859 microparticle Substances 0.000 description 1
238000002156 mixing Methods 0.000 description 1
239000000203 mixture Substances 0.000 description 1
238000012544 monitoring process Methods 0.000 description 1
230000009871 nonspecific binding Effects 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
150000007523 nucleic acids Chemical class 0.000 description 1
239000013307 optical fiber Substances 0.000 description 1
150000002894 organic compounds Chemical class 0.000 description 1
239000000123 paper Substances 0.000 description 1
230000036961 partial effect Effects 0.000 description 1
238000004735 phosphorescence spectroscopy Methods 0.000 description 1
230000000704 physical effect Effects 0.000 description 1
239000000049 pigment Substances 0.000 description 1
238000006116 polymerization reaction Methods 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
102000004196 processed proteins & peptides Human genes 0.000 description 1
108090000765 processed proteins & peptides Proteins 0.000 description 1
238000012545 processing Methods 0.000 description 1
238000011002 quantification Methods 0.000 description 1
230000009467 reduction Effects 0.000 description 1
230000002829 reductive effect Effects 0.000 description 1
238000002310 reflectometry Methods 0.000 description 1
230000007261 regionalization Effects 0.000 description 1
238000011160 research Methods 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
238000004062 sedimentation Methods 0.000 description 1
238000001228 spectrum Methods 0.000 description 1
239000012536 storage buffer Substances 0.000 description 1
239000000126 substance Substances 0.000 description 1
238000006467 substitution reaction Methods 0.000 description 1
YOEWQQVKRJEPAE-UHFFFAOYSA-L succinylcholine chloride (anhydrous) Chemical compound [Cl-].[Cl-].C[N+](C)(C)CCOC(=O)CCC(=O)OCC[N+](C)(C)C YOEWQQVKRJEPAE-UHFFFAOYSA-L 0.000 description 1
230000009897 systematic effect Effects 0.000 description 1
230000001225 therapeutic effect Effects 0.000 description 1
SWGJCIMEBVHMTA-UHFFFAOYSA-K trisodium;6-oxido-4-sulfo-5-[(4-sulfonatonaphthalen-1-yl)diazenyl]naphthalene-2-sulfonate Chemical compound [Na+].[Na+].[Na+].C1=CC=C2C(N=NC3=C4C(=CC(=CC4=CC=C3O)S([O-])(=O)=O)S([O-])(=O)=O)=CC=C(S([O-])(=O)=O)C2=C1 SWGJCIMEBVHMTA-UHFFFAOYSA-K 0.000 description 1
210000002700 urine Anatomy 0.000 description 1
229910052724 xenon Inorganic materials 0.000 description 1
FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/4788—Diffraction
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
- G01N21/45—Refractivity; Phase-affecting properties, e.g. optical path length using interferometric methods; using Schlieren methods
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Immunology (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Hematology (AREA)
Urology & Nephrology (AREA)
Analytical Chemistry (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Microbiology (AREA)
Cell Biology (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
Biotechnology (AREA)
Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

CA002149784A 1992-11-24 1992-11-24 Detection de ligands par modulation d'interference Abandoned CA2149784A1 (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA002149784A CA2149784A1 (fr)	1992-11-24	1992-11-24	Detection de ligands par modulation d'interference

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA002149784A CA2149784A1 (fr)	1992-11-24	1992-11-24	Detection de ligands par modulation d'interference

Publications (1)

Publication Number	Publication Date
CA2149784A1 true CA2149784A1 (fr)	1994-06-09

Family

ID=4155872

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002149784A Abandoned CA2149784A1 (fr)	1992-11-24	1992-11-24	Detection de ligands par modulation d'interference

Country Status (1)

Country	Link
CA (1)	CA2149784A1 (fr)

1992
- 1992-11-24 CA CA002149784A patent/CA2149784A1/fr not_active Abandoned

Legal Events

Date	Code	Title	Description
2000-11-24	FZDE	Discontinued

Publication	Publication Date	Title
EP0670043B1 (fr)	1999-01-27	Dosage de ligand par modulation d'interference
EP0167335B1 (fr)	1991-11-27	Méthode de détection d'une réaction de liaison entre un ligand et un antiligand
USRE33581E (en)	1991-04-30	Immunoassay using optical interference detection
CA2469015C (fr)	2014-07-29	Procede de visionnement et d'analyse des resultats de diagnostics par diffraction
US7102752B2 (en)	2006-09-05	Systems to view and analyze the results from diffraction-based diagnostics
US8283156B2 (en)	2012-10-09	Method and apparatus for assay based on light diffraction
US7663092B2 (en)	2010-02-16	Method and apparatus for phase contrast quadrature interferometric detection of an immunoassay
JPS62503053A (ja)	1987-12-03	物質の選択的検出および測定物質内の屈折率変化検知を行なう光学センサ
JPS5855758A (ja)	1983-04-02	生物学的成分を測定する方法、手段および装置
AU604830B2 (en)	1991-01-03	Diffraction immunoassay and reagents
RU2181487C2 (ru)	2002-04-20	Способ оптического детектирования присоединения вещественного компонента к сенсорному материалу на основе биологического, химического или физического взаимодействия и устройство для его осуществления (варианты)
GB2273772A (en)	1994-06-29	Detection of macromolecules utilising light diffraction
AU4190099A (en)	1999-11-29	Enumeration method of analyte detection
JPH01502930A (ja)	1989-10-05	改良された分析技術およびそのための装置
JPH04233465A (ja)	1992-08-21	散乱光検知免疫測定
JP2014531595A (ja)	2014-11-27	試料配列の自己対照型検出及び撮像のためのシステム及び方法
US4988630A (en)	1991-01-29	Multiple beam laser instrument for measuring agglutination reactions
EP0515370B1 (fr)	1995-03-01	Systeme d'immunoanalyse interferometrique a phase solide
JP2572829B2 (ja)	1997-01-16	導波管センサー
EP0254430B1 (fr)	1993-09-08	Un système d'essai immunologique utilisant de la lumière dispersée
AU670252B2 (en)	1996-07-11	Ligand assay using interference modulation
CA2149784A1 (fr)	1994-06-09	Detection de ligands par modulation d'interference
EP1105735B1 (fr)	2007-10-24	Essais de liaison mettant en oeuvre la resonance optique de particules colloidales