CA2178590A1 - Reactifs et procedes de detection du methotrexate - Google Patents

Reactifs et procedes de detection du methotrexate

Info

Publication number
CA2178590A1
CA2178590A1 CA 2178590 CA2178590A CA2178590A1 CA 2178590 A1 CA2178590 A1 CA 2178590A1 CA 2178590 CA2178590 CA 2178590 CA 2178590 A CA2178590 A CA 2178590A CA 2178590 A1 CA2178590 A1 CA 2178590A1
Authority
CA
Canada
Prior art keywords
antibody
methotrexate
cell line
test sample
hydroxymethotrexate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2178590
Other languages
English (en)
Inventor
Joan D. Tyner
Sherri L. Juveland
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2178590A1 publication Critical patent/CA2178590A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un anticorps monoclonal murin IgG utilisé dans la détection du méthotrexate dans les liquides biologiques. Cet anticorps monoclonal désigné par 4-354-178 peut être utilisé dans une technique de détection du méthotrexate, de préférence un dosage immunologique à polarisation de fluorescence (FPIA) de l'urine. L'anticorps monoclonal 4-351-178 se caractérise par l'absence de réactivité croisée détectable avec le 7-hydroxyméthotrexate. L'invention porte également sur une lignée cellulaire d'hybridome, ATC HB 11467, produisant l'anticorps monoclonal selon l'invention.
CA 2178590 1993-12-10 1994-11-22 Reactifs et procedes de detection du methotrexate Abandoned CA2178590A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16512193A 1993-12-10 1993-12-10
US08/165,121 1993-12-10

Publications (1)

Publication Number Publication Date
CA2178590A1 true CA2178590A1 (fr) 1995-06-15

Family

ID=22597511

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2178590 Abandoned CA2178590A1 (fr) 1993-12-10 1994-11-22 Reactifs et procedes de detection du methotrexate

Country Status (5)

Country Link
EP (1) EP0733102A1 (fr)
JP (1) JPH09506260A (fr)
AU (1) AU1186695A (fr)
CA (1) CA2178590A1 (fr)
WO (1) WO1995016026A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107219357A (zh) * 2017-04-21 2017-09-29 中国人民解放军第三军医大学第附属医院 一种检测甲氨蝶呤的elisa试剂盒及其应用

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5710009A (en) * 1994-02-17 1998-01-20 Serex, Inc. Receptor:release ligand (reland) complexes and release assays using said reland and methods and kits based thereon
AU2678997A (en) * 1996-04-19 1997-11-12 Lexigen Pharmaceuticals Corp. Inhibition of the binding of human ige to its receptor by tetracyclic compounds for the alleviation of ige-mediated immune response
EP3002299A1 (fr) 2008-06-03 2016-04-06 AbbVie Inc. Immunoglobulines à deux domaines variables et leurs utilisations
AR078254A1 (es) * 2009-09-01 2011-10-26 Abbott Lab Inmunoglobulinas con dominio variable dual y usos de las mismas
US8735546B2 (en) 2010-08-03 2014-05-27 Abbvie Inc. Dual variable domain immunoglobulins and uses thereof
PH12013500337A1 (en) 2010-08-26 2017-08-23 Abbvie Inc Dual variable domain immunoglobulins and uses thereof
US9120870B2 (en) 2011-12-30 2015-09-01 Abbvie Inc. Dual specific binding proteins directed against IL-13 and IL-17
AU2013337775B2 (en) 2012-11-01 2017-03-30 Abbvie Inc. Anti-VEGF/DLL4 dual variable domain immunoglobulins and uses thereof
EP2970459A2 (fr) 2013-03-15 2016-01-20 AbbVie Inc. Protéines de liaison spécifiques à domaines variables doubles dirigées contre il -1beta et il -17
US10093733B2 (en) 2014-12-11 2018-10-09 Abbvie Inc. LRP-8 binding dual variable domain immunoglobulin proteins
CN104569373B (zh) * 2015-01-27 2016-08-17 苏州博源医疗科技有限公司 一种甲氨蝶呤均相酶免疫检测试剂及其制备和检测方法
TW201710286A (zh) 2015-06-15 2017-03-16 艾伯維有限公司 抗ｖｅｇｆ、ｐｄｇｆ及／或其受體之結合蛋白
US10675364B2 (en) * 2015-06-23 2020-06-09 The Board Of Trustees Of The Leland Stanford Junior University Rhodol fluorophores for near-infrared imaging
WO2017007889A1 (fr) * 2015-07-08 2017-01-12 Defined Diagnostics, Llc Composés et méthodes pour la détection du méthotrexate
CN108614107A (zh) * 2016-12-12 2018-10-02 上海复星长征医学科学有限公司 一种甲氨蝶呤胶乳免疫比浊测定试剂盒及其制备方法
CN110357886B (zh) * 2018-04-09 2022-06-24 浙江准策生物技术有限公司 甲氨蝶呤半抗原和完全抗原及其制备方法和应用

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107219357A (zh) * 2017-04-21 2017-09-29 中国人民解放军第三军医大学第附属医院 一种检测甲氨蝶呤的elisa试剂盒及其应用

Also Published As

Publication number Publication date
WO1995016026A1 (fr) 1995-06-15
AU1186695A (en) 1995-06-27
EP0733102A1 (fr) 1996-09-25
JPH09506260A (ja) 1997-06-24

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Dead