CA2228769C - Method of purifying, stabilising or isolating nucleic acids contained in biological matter - Google Patents

Method of purifying, stabilising or isolating nucleic acids contained in biological matter Download PDF

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Publication number
CA2228769C
CA2228769C CA2228769A CA2228769A CA2228769C CA 2228769 C CA2228769 C CA 2228769C CA 2228769 A CA2228769 A CA 2228769A CA 2228769 A CA2228769 A CA 2228769A CA 2228769 C CA2228769 C CA 2228769C
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CA
Canada
Prior art keywords
nucleic acids
nucleic
adsorption matrix
acid
reagent kit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2228769A
Other languages
English (en)
French (fr)
Other versions
CA2228769A1 (en
Inventor
Oliver Muller
Rainer Deuter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Original Assignee
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft zur Foerderung der Wissenschaften eV filed Critical Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Publication of CA2228769A1 publication Critical patent/CA2228769A1/en
Application granted granted Critical
Publication of CA2228769C publication Critical patent/CA2228769C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Processing Of Solid Wastes (AREA)
  • Detergent Compositions (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
CA2228769A 1995-08-16 1996-08-14 Method of purifying, stabilising or isolating nucleic acids contained in biological matter Expired - Lifetime CA2228769C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19530132A DE19530132C2 (de) 1995-08-16 1995-08-16 Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien
DE19530132.3 1995-08-16
PCT/EP1996/003595 WO1997007239A1 (de) 1995-08-16 1996-08-14 Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien

Publications (2)

Publication Number Publication Date
CA2228769A1 CA2228769A1 (en) 1997-02-27
CA2228769C true CA2228769C (en) 2011-04-12

Family

ID=7769624

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2228769A Expired - Lifetime CA2228769C (en) 1995-08-16 1996-08-14 Method of purifying, stabilising or isolating nucleic acids contained in biological matter

Country Status (9)

Country Link
US (1) US6084091A (2)
EP (1) EP0851937B1 (2)
JP (1) JP4264133B2 (2)
AT (1) ATE215611T1 (2)
AU (1) AU712331B2 (2)
CA (1) CA2228769C (2)
DE (2) DE19530132C2 (2)
DK (1) DK0851937T3 (2)
WO (1) WO1997007239A1 (2)

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US7981612B2 (en) 1999-12-07 2011-07-19 Mayo Foundation For Medical Education And Research Methods of screening for supracolonic neoplasms based on stool samples containing a nucleic acid marker indicative of a neoplasm

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AU7816898A (en) * 1997-06-16 1999-01-04 Exact Laboratories, Inc. Methods for stool sample preparation
US6406857B1 (en) 1997-06-16 2002-06-18 Exact Sciences Corporation Methods for stool sample preparation
US6268136B1 (en) 1997-06-16 2001-07-31 Exact Science Corporation Methods for stool sample preparation
US6241980B1 (en) * 1997-11-04 2001-06-05 Becton, Dickinson And Company Sample processing method using ion exchange resin
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
EP0939118A1 (en) * 1998-02-20 1999-09-01 Universiteit Maastricht Method for isolating DNA and RNA from faeces
JP2002510747A (ja) 1998-04-08 2002-04-09 メタボリックス，インコーポレイテッド バイオポリマーの分離および精製のための方法
AU746769B2 (en) 1998-06-09 2002-05-02 Metabolix, Inc. Methods and apparatus for the production of amorphous polymer suspensions
DE19900638C2 (de) * 1999-01-11 2002-12-19 Max Planck Gesellschaft Methode zur Isolierung von DNA aus biologischen Materialien
AU752817B2 (en) * 1999-02-25 2002-10-03 Exact Sciences Corporation Methods for preserving DNA integrity
CA2366778C (en) * 1999-04-09 2008-07-22 Exact Sciences Corporation Methods for detecting nucleic acids indicative of cancer
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6383783B1 (en) 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
US6919174B1 (en) * 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
CA2399475C (en) * 2000-02-04 2011-04-12 Qiagen Gmbh Isolation of nucleic acid from stool samples and other biological materials which are rich in inhibitors
AU2001238616A1 (en) * 2000-02-23 2001-09-03 Wen Shao Rapid nucleic acid separation, isolation and purification methods
GB0015777D0 (en) * 2000-06-28 2000-08-16 Tayside University Hospitals N DNA Extraction
CA2465961A1 (en) * 2001-11-06 2003-05-15 Cortex Biochem, Inc. Isolation and purification of nucleic acids
GB0130821D0 (en) * 2001-12-24 2002-02-06 Tayside University Hospitals N DNA screening
WO2003071252A2 (en) 2002-02-15 2003-08-28 Exact Sciences Corporation Methods for analysis of molecular events
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
US20040259101A1 (en) * 2003-06-20 2004-12-23 Shuber Anthony P. Methods for disease screening
WO2005026347A1 (en) * 2003-09-09 2005-03-24 Fuji Photo Film Co., Ltd. Method for isolating and purifying a nucleic acid
JP2007508922A (ja) * 2003-10-02 2007-04-12 ミシシッピ・ステイト・ユニバーシティ 廃水処理プラント汚泥からのバイオディーゼル燃料及び他の有用な化学物質の生成
US20050130177A1 (en) * 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
US20050142571A1 (en) * 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using solid phase material
US7939249B2 (en) 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US7727710B2 (en) 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface
WO2005068662A1 (en) * 2003-12-30 2005-07-28 Sigma-Aldrich Co. Rapid preparation of nucleic acids by enzymatic digestion
US8426126B2 (en) * 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
WO2005111244A2 (en) * 2004-05-10 2005-11-24 Exact Sciences Corporation Methods for detecting a mutant nucleic acid
US7981607B2 (en) 2004-08-27 2011-07-19 Esoterix Genetic Laboratories LLC Method for detecting recombinant event
US7195878B2 (en) * 2004-09-30 2007-03-27 Cryo-Genomics Device and method for fecal testing
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
US9777314B2 (en) * 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
JP4819427B2 (ja) 2005-07-15 2011-11-24 株式会社リコー 画像形成装置、画像形成方法、及びプロセスカートリッジ
JP4590344B2 (ja) 2005-11-21 2010-12-01 株式会社リコー 静電潜像担持体及びそれを用いた画像形成装置、プロセスカートリッジ及び画像形成方法
JP2009011247A (ja) * 2007-07-05 2009-01-22 Sumitomo Bakelite Co Ltd 遺伝子の検出方法
US7759112B2 (en) * 2007-10-31 2010-07-20 Akonni Biosystems, Inc. Apparatus, system, and method for purifying nucleic acids
US20090111193A1 (en) 2007-10-31 2009-04-30 Cooney Christopher G Sample preparation device
US9428746B2 (en) 2007-10-31 2016-08-30 Akonni Biosystems, Inc. Method and kit for purifying nucleic acids
US10125388B2 (en) 2007-10-31 2018-11-13 Akonni Biosystems, Inc. Integrated sample processing system
US9347055B2 (en) 2007-11-05 2016-05-24 Eiken Kagaku Kabushiki Kaisha Method and kit for preparation of sample for use in nucleic acid amplification
JP5599013B2 (ja) * 2008-04-03 2014-10-01 キヤノン株式会社 血液検体からの微生物核酸の抽出方法
CN102105583A (zh) * 2008-07-23 2011-06-22 奥林巴斯株式会社 从粪便试样中回收核酸的方法、核酸分析方法以及粪便试样处理装置
WO2010028382A2 (en) * 2008-09-05 2010-03-11 Mayo Foundation For Medical Education And Research Collecting and processing complex macromolecular mixtures
JPWO2010134245A1 (ja) * 2009-05-20 2012-11-08 オリンパス株式会社 哺乳細胞由来核酸の回収方法、核酸解析方法、及び採便用キット
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
LT2521780T (lt) 2010-01-07 2018-02-12 Bigtec Private Limited Neklerūgščių išskyrimo būdas ir rinkinys
JP5743701B2 (ja) * 2011-05-10 2015-07-01 英二 小西 Ｒｎａ検出用試薬およびｒｎａ検出方法
JP5743704B2 (ja) * 2011-05-12 2015-07-01 住友大阪セメント株式会社 Ｒｎａ検出用試薬およびｒｎａ検出方法
CN106442039B (zh) 2011-06-19 2020-08-07 阿博根公司 用于样品采集的装置、溶液和方法
BR112014005107A2 (pt) * 2011-09-06 2017-04-18 North-West Univ método de preparação de material biológico
US9234875B2 (en) 2011-11-04 2016-01-12 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
US9658195B2 (en) 2012-02-15 2017-05-23 Bio-Rad Laboratories, Inc. Electronic control of pH and ionic strength
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
CA2941764C (en) 2014-03-07 2023-10-24 Dna Genotek Inc. Composition and method for stabilizing nucleic acids in biological samples
WO2017044827A1 (en) 2015-09-10 2017-03-16 Life Technologies Corporation Purification of nucleic acid from environmental or biological samples
CN113832141B (zh) * 2021-09-30 2024-03-08 苏州唯善生物科技有限公司 粪便人源基因组dna特异性纯化方法及试剂盒

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US5334499A (en) * 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7981612B2 (en) 1999-12-07 2011-07-19 Mayo Foundation For Medical Education And Research Methods of screening for supracolonic neoplasms based on stool samples containing a nucleic acid marker indicative of a neoplasm

Also Published As

Publication number Publication date
JPH11511020A (ja) 1999-09-28
AU6821696A (en) 1997-03-12
DE19530132A1 (de) 1997-02-20
DE59609027D1 (de) 2002-05-08
US6084091A (en) 2000-07-04
ATE215611T1 (de) 2002-04-15
JP4264133B2 (ja) 2009-05-13
WO1997007239A1 (de) 1997-02-27
CA2228769A1 (en) 1997-02-27
EP0851937A1 (de) 1998-07-08
EP0851937B1 (de) 2002-04-03
DE19530132C2 (de) 1998-07-16
AU712331B2 (en) 1999-11-04
DK0851937T3 (da) 2002-06-24

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Effective date: 20160815