CA2228769C - Method of purifying, stabilising or isolating nucleic acids contained in biological matter - Google Patents

Method of purifying, stabilising or isolating nucleic acids contained in biological matter Download PDF

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Publication number
CA2228769C
CA2228769C CA2228769A CA2228769A CA2228769C CA 2228769 C CA2228769 C CA 2228769C CA 2228769 A CA2228769 A CA 2228769A CA 2228769 A CA2228769 A CA 2228769A CA 2228769 C CA2228769 C CA 2228769C
Authority
CA
Canada
Prior art keywords
nucleic acids
nucleic
adsorption matrix
acid
reagent kit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2228769A
Other languages
English (en)
French (fr)
Other versions
CA2228769A1 (en
Inventor
Oliver Muller
Rainer Deuter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max-Planck-Gesellschaft zur Förderung der Wissenschaften eV
Original Assignee
Max-Planck-Gesellschaft zur Förderung der Wissenschaften eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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Publication of CA2228769A1 publication Critical patent/CA2228769A1/en
Application granted granted Critical
Publication of CA2228769C publication Critical patent/CA2228769C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Detergent Compositions (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Processing Of Solid Wastes (AREA)
CA2228769A 1995-08-16 1996-08-14 Method of purifying, stabilising or isolating nucleic acids contained in biological matter Expired - Lifetime CA2228769C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19530132.3 1995-08-16
DE19530132A DE19530132C2 (de) 1995-08-16 1995-08-16 Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien
PCT/EP1996/003595 WO1997007239A1 (de) 1995-08-16 1996-08-14 Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien

Publications (2)

Publication Number Publication Date
CA2228769A1 CA2228769A1 (en) 1997-02-27
CA2228769C true CA2228769C (en) 2011-04-12

Family

ID=7769624

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2228769A Expired - Lifetime CA2228769C (en) 1995-08-16 1996-08-14 Method of purifying, stabilising or isolating nucleic acids contained in biological matter

Country Status (9)

Country Link
US (1) US6084091A (2)
EP (1) EP0851937B1 (2)
JP (1) JP4264133B2 (2)
AT (1) ATE215611T1 (2)
AU (1) AU712331B2 (2)
CA (1) CA2228769C (2)
DE (2) DE19530132C2 (2)
DK (1) DK0851937T3 (2)
WO (1) WO1997007239A1 (2)

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US7981612B2 (en) 1999-12-07 2011-07-19 Mayo Foundation For Medical Education And Research Methods of screening for supracolonic neoplasms based on stool samples containing a nucleic acid marker indicative of a neoplasm

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WO1998058081A1 (en) * 1997-06-16 1998-12-23 Exact Laboratories, Inc. Methods for stool sample preparation
US6268136B1 (en) * 1997-06-16 2001-07-31 Exact Science Corporation Methods for stool sample preparation
US6406857B1 (en) 1997-06-16 2002-06-18 Exact Sciences Corporation Methods for stool sample preparation
US6241980B1 (en) 1997-11-04 2001-06-05 Becton, Dickinson And Company Sample processing method using ion exchange resin
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
EP0939118A1 (en) * 1998-02-20 1999-09-01 Universiteit Maastricht Method for isolating DNA and RNA from faeces
JP2002510747A (ja) 1998-04-08 2002-04-09 メタボリックス，インコーポレイテッド バイオポリマーの分離および精製のための方法
ATE336537T1 (de) 1998-06-09 2006-09-15 Metabolix Inc Verfahren und vorrichtung zur herstellung von amorphen polymersuspensionen
DE19900638C2 (de) * 1999-01-11 2002-12-19 Max Planck Gesellschaft Methode zur Isolierung von DNA aus biologischen Materialien
WO2000050640A1 (en) * 1999-02-25 2000-08-31 Exact Laboratories, Inc. Methods for preserving dna integrity
US6964846B1 (en) 1999-04-09 2005-11-15 Exact Sciences Corporation Methods for detecting nucleic acids indicative of cancer
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6383783B1 (en) 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
US6919174B1 (en) * 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
AU778713B2 (en) * 2000-02-04 2004-12-16 Qiagen Gmbh Nucleic acid isolation from stool samples and other inhibitor-rich biological materials
AU2001238616A1 (en) * 2000-02-23 2001-09-03 Wen Shao Rapid nucleic acid separation, isolation and purification methods
GB0015777D0 (en) * 2000-06-28 2000-08-16 Tayside University Hospitals N DNA Extraction
DE60230788D1 (de) * 2001-11-06 2009-02-26 Cortex Biochem Inc Isolierung und aufreinigung von nukleinsäuren
GB0130821D0 (en) * 2001-12-24 2002-02-06 Tayside University Hospitals N DNA screening
US7776524B2 (en) 2002-02-15 2010-08-17 Genzyme Corporation Methods for analysis of molecular events
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
US20040259101A1 (en) * 2003-06-20 2004-12-23 Shuber Anthony P. Methods for disease screening
WO2005026347A1 (en) * 2003-09-09 2005-03-24 Fuji Photo Film Co., Ltd. Method for isolating and purifying a nucleic acid
EP1670880A4 (en) * 2003-10-02 2007-04-04 Univ Mississippi METHOD FOR PRODUCING BIODIESEL AND OTHER VALUABLE CHEMICALS FROM SLUDGE FROM WASTEWATER TREATMENT PLANTS
US20050130177A1 (en) * 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
US7727710B2 (en) 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface
US7939249B2 (en) 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US20050142571A1 (en) * 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using solid phase material
US20050164260A1 (en) * 2003-12-30 2005-07-28 Sigma-Aldrich Co. Rapid preparation of nucleic acids by enzymatic digestion
US8426126B2 (en) 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
WO2005111244A2 (en) * 2004-05-10 2005-11-24 Exact Sciences Corporation Methods for detecting a mutant nucleic acid
WO2006026654A2 (en) 2004-08-27 2006-03-09 Exact Sciences Corporation Method for detecting a recombinant event
US7195878B2 (en) * 2004-09-30 2007-03-27 Cryo-Genomics Device and method for fecal testing
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
US9777314B2 (en) * 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
JP4819427B2 (ja) 2005-07-15 2011-11-24 株式会社リコー 画像形成装置、画像形成方法、及びプロセスカートリッジ
JP4590344B2 (ja) 2005-11-21 2010-12-01 株式会社リコー 静電潜像担持体及びそれを用いた画像形成装置、プロセスカートリッジ及び画像形成方法
JP2009011247A (ja) * 2007-07-05 2009-01-22 Sumitomo Bakelite Co Ltd 遺伝子の検出方法
US7759112B2 (en) * 2007-10-31 2010-07-20 Akonni Biosystems, Inc. Apparatus, system, and method for purifying nucleic acids
US10125388B2 (en) 2007-10-31 2018-11-13 Akonni Biosystems, Inc. Integrated sample processing system
US20090111193A1 (en) * 2007-10-31 2009-04-30 Cooney Christopher G Sample preparation device
US9428746B2 (en) 2007-10-31 2016-08-30 Akonni Biosystems, Inc. Method and kit for purifying nucleic acids
CA2703884C (en) * 2007-11-05 2015-03-17 Eiken Kagaku Kabushiki Kaisha Method and kit for preparation of sample for use in nucleic acid amplification
JP5599013B2 (ja) * 2008-04-03 2014-10-01 キヤノン株式会社 血液検体からの微生物核酸の抽出方法
CN102105583A (zh) * 2008-07-23 2011-06-22 奥林巴斯株式会社 从粪便试样中回收核酸的方法、核酸分析方法以及粪便试样处理装置
WO2010028382A2 (en) * 2008-09-05 2010-03-11 Mayo Foundation For Medical Education And Research Collecting and processing complex macromolecular mixtures
JPWO2010134245A1 (ja) * 2009-05-20 2012-11-08 オリンパス株式会社 哺乳細胞由来核酸の回収方法、核酸解析方法、及び採便用キット
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
US8986976B2 (en) 2010-01-07 2015-03-24 Bigtec Private Limited Method for isolation of nucleic acids and a kit thereof
JP5743701B2 (ja) * 2011-05-10 2015-07-01 英二 小西 Ｒｎａ検出用試薬およびｒｎａ検出方法
JP5743704B2 (ja) * 2011-05-12 2015-07-01 住友大阪セメント株式会社 Ｒｎａ検出用試薬およびｒｎａ検出方法
US9442046B2 (en) 2011-06-19 2016-09-13 Abogen, Inc. Device for sample collection
WO2013035062A1 (en) * 2011-09-06 2013-03-14 North-West University A method of preparing biological material
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
US9234875B2 (en) 2011-11-04 2016-01-12 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
EP2814597A4 (en) 2012-02-15 2015-10-28 Bio Rad Laboratories ELECTRONIC CONTROL OF PH-VALUE AND ION STRENGTH
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
KR102392407B1 (ko) 2014-03-07 2022-04-29 디엔에이 제노텍 인코퍼레이티드 생물학적 샘플에서 핵산을 안정화하기 위한 조성물 및 방법
EP3347491B1 (en) 2015-09-10 2023-08-23 Life Technologies Corporation Purification of nucleic acid from environmental or biological samples
CN113832141B (zh) * 2021-09-30 2024-03-08 苏州唯善生物科技有限公司 粪便人源基因组dna特异性纯化方法及试剂盒

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7981612B2 (en) 1999-12-07 2011-07-19 Mayo Foundation For Medical Education And Research Methods of screening for supracolonic neoplasms based on stool samples containing a nucleic acid marker indicative of a neoplasm

Also Published As

Publication number Publication date
DK0851937T3 (da) 2002-06-24
EP0851937B1 (de) 2002-04-03
WO1997007239A1 (de) 1997-02-27
JP4264133B2 (ja) 2009-05-13
US6084091A (en) 2000-07-04
DE59609027D1 (de) 2002-05-08
DE19530132C2 (de) 1998-07-16
EP0851937A1 (de) 1998-07-08
AU6821696A (en) 1997-03-12
ATE215611T1 (de) 2002-04-15
AU712331B2 (en) 1999-11-04
JPH11511020A (ja) 1999-09-28
DE19530132A1 (de) 1997-02-20
CA2228769A1 (en) 1997-02-27

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Effective date: 20160815