ATE215611T1 - Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien - Google Patents

Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien

Info

Publication number
ATE215611T1
ATE215611T1 AT96928466T AT96928466T ATE215611T1 AT E215611 T1 ATE215611 T1 AT E215611T1 AT 96928466 T AT96928466 T AT 96928466T AT 96928466 T AT96928466 T AT 96928466T AT E215611 T1 ATE215611 T1 AT E215611T1
Authority
AT
Austria
Prior art keywords
pct
nucleic acids
stabilization
isolation
purification
Prior art date
Application number
AT96928466T
Other languages
German (de)
English (en)
Inventor
Oliver Mueller
Rainer Deuter
Original Assignee
Max Planck Gesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft filed Critical Max Planck Gesellschaft
Application granted granted Critical
Publication of ATE215611T1 publication Critical patent/ATE215611T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Processing Of Solid Wastes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
AT96928466T 1995-08-16 1996-08-14 Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien ATE215611T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19530132A DE19530132C2 (de) 1995-08-16 1995-08-16 Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien
PCT/EP1996/003595 WO1997007239A1 (de) 1995-08-16 1996-08-14 Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien

Publications (1)

Publication Number Publication Date
ATE215611T1 true ATE215611T1 (de) 2002-04-15

Family

ID=7769624

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96928466T ATE215611T1 (de) 1995-08-16 1996-08-14 Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien

Country Status (9)

Country Link
US (1) US6084091A (2)
EP (1) EP0851937B1 (2)
JP (1) JP4264133B2 (2)
AT (1) ATE215611T1 (2)
AU (1) AU712331B2 (2)
CA (1) CA2228769C (2)
DE (2) DE19530132C2 (2)
DK (1) DK0851937T3 (2)
WO (1) WO1997007239A1 (2)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19737917B4 (de) * 1996-08-26 2008-11-06 InViTek Gesellschaft für Biotechnik & Biodesign mbH Verfahren zum Nachweis klinisch relevanter Veränderungen der DNS-Sequenz des Ki-ras-Onkogens, seine Verwendung und Testkit zur Früherkennung von Tumoren
US6406857B1 (en) 1997-06-16 2002-06-18 Exact Sciences Corporation Methods for stool sample preparation
WO1998058081A1 (en) * 1997-06-16 1998-12-23 Exact Laboratories, Inc. Methods for stool sample preparation
US6268136B1 (en) 1997-06-16 2001-07-31 Exact Science Corporation Methods for stool sample preparation
US6241980B1 (en) 1997-11-04 2001-06-05 Becton, Dickinson And Company Sample processing method using ion exchange resin
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
EP0939118A1 (en) * 1998-02-20 1999-09-01 Universiteit Maastricht Method for isolating DNA and RNA from faeces
JP2002510747A (ja) 1998-04-08 2002-04-09 メタボリックス，インコーポレイテッド バイオポリマーの分離および精製のための方法
WO1999064498A1 (en) 1998-06-09 1999-12-16 Metabolix, Inc. Methods and apparatus for the production of amorphous polymer suspensions
DE19900638C2 (de) * 1999-01-11 2002-12-19 Max Planck Gesellschaft Methode zur Isolierung von DNA aus biologischen Materialien
CA2362251C (en) * 1999-02-25 2008-06-17 Exact Sciences Corporation Methods for preserving dna integrity
DE60029092T2 (de) 1999-04-09 2007-02-01 Exact Sciences Corp., Marlborough Verfahren zur detektion von nukleinsäuren, welche auf krebs hinweisen
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6383783B1 (en) 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
DE60043896D1 (de) 1999-12-07 2010-04-08 Exact Sciences Corp Verfahren zum nachweis von lungenneoplasmen in fäkalen proben
JP4776133B2 (ja) * 2000-02-04 2011-09-21 キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング 阻害物質に富む糞便試料及び他の生物学的材料からの核酸の単離
AU2001238616A1 (en) * 2000-02-23 2001-09-03 Wen Shao Rapid nucleic acid separation, isolation and purification methods
GB0015777D0 (en) * 2000-06-28 2000-08-16 Tayside University Hospitals N DNA Extraction
DE60230788D1 (de) * 2001-11-06 2009-02-26 Cortex Biochem Inc Isolierung und aufreinigung von nukleinsäuren
GB0130821D0 (en) * 2001-12-24 2002-02-06 Tayside University Hospitals N DNA screening
WO2003071252A2 (en) 2002-02-15 2003-08-28 Exact Sciences Corporation Methods for analysis of molecular events
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
US20040259101A1 (en) * 2003-06-20 2004-12-23 Shuber Anthony P. Methods for disease screening
EP1664295A4 (en) * 2003-09-09 2007-12-05 Fujifilm Corp PROCESS FOR INSULATING AND PURIFYING NUCLEIC ACID
JP2007508922A (ja) * 2003-10-02 2007-04-12 ミシシッピ・ステイト・ユニバーシティ 廃水処理プラント汚泥からのバイオディーゼル燃料及び他の有用な化学物質の生成
US20050130177A1 (en) * 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
US20050142571A1 (en) * 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using solid phase material
US7939249B2 (en) 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US7727710B2 (en) 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface
WO2005068662A1 (en) * 2003-12-30 2005-07-28 Sigma-Aldrich Co. Rapid preparation of nucleic acids by enzymatic digestion
US8426126B2 (en) * 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
US20080241827A1 (en) * 2004-05-10 2008-10-02 Exact Sciences Corporation Methods For Detecting A Mutant Nucleic Acid
US7981607B2 (en) * 2004-08-27 2011-07-19 Esoterix Genetic Laboratories LLC Method for detecting recombinant event
US7195878B2 (en) * 2004-09-30 2007-03-27 Cryo-Genomics Device and method for fecal testing
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
WO2007044071A2 (en) * 2005-04-21 2007-04-19 Exact Sciences Corporation Analysis of heterogeneous nucleic acid samples
JP4819427B2 (ja) 2005-07-15 2011-11-24 株式会社リコー 画像形成装置、画像形成方法、及びプロセスカートリッジ
JP4590344B2 (ja) 2005-11-21 2010-12-01 株式会社リコー 静電潜像担持体及びそれを用いた画像形成装置、プロセスカートリッジ及び画像形成方法
JP2009011247A (ja) * 2007-07-05 2009-01-22 Sumitomo Bakelite Co Ltd 遺伝子の検出方法
US7759112B2 (en) * 2007-10-31 2010-07-20 Akonni Biosystems, Inc. Apparatus, system, and method for purifying nucleic acids
US10125388B2 (en) 2007-10-31 2018-11-13 Akonni Biosystems, Inc. Integrated sample processing system
US20090111193A1 (en) * 2007-10-31 2009-04-30 Cooney Christopher G Sample preparation device
US9428746B2 (en) 2007-10-31 2016-08-30 Akonni Biosystems, Inc. Method and kit for purifying nucleic acids
JP5290987B2 (ja) * 2007-11-05 2013-09-18 栄研化学株式会社 核酸増幅用サンプルの調製方法及び調製キット
JP5599013B2 (ja) * 2008-04-03 2014-10-01 キヤノン株式会社 血液検体からの微生物核酸の抽出方法
WO2010010914A1 (ja) * 2008-07-23 2010-01-28 オリンパス株式会社 糞便試料からの核酸回収方法、核酸解析方法及び糞便試料処理装置
WO2010028382A2 (en) * 2008-09-05 2010-03-11 Mayo Foundation For Medical Education And Research Collecting and processing complex macromolecular mixtures
JPWO2010134245A1 (ja) * 2009-05-20 2012-11-08 オリンパス株式会社 哺乳細胞由来核酸の回収方法、核酸解析方法、及び採便用キット
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
PL2521780T3 (pl) 2010-01-07 2018-03-30 Bigtec Private Limited Sposób i zestaw do izolowania kwasów nukleinowych
JP5743701B2 (ja) * 2011-05-10 2015-07-01 英二 小西 Ｒｎａ検出用試薬およびｒｎａ検出方法
JP5743704B2 (ja) * 2011-05-12 2015-07-01 住友大阪セメント株式会社 Ｒｎａ検出用試薬およびｒｎａ検出方法
EP3150702B1 (en) 2011-06-19 2021-05-19 DNA Genotek, Inc. Devices, solutions and methods for sample collection
CA2847847A1 (en) * 2011-09-06 2013-03-14 North-West University A method of preparing biological material
WO2013067509A1 (en) * 2011-11-04 2013-05-10 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
WO2013123425A1 (en) 2012-02-15 2013-08-22 Bio-Rad Laboratories, Inc. Electronic control of ph and ionic strength
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
WO2015131291A1 (en) 2014-03-07 2015-09-11 Dna Genotek Inc. Composition and method for stabilizing nucleic acids in biological samples
WO2017044827A1 (en) 2015-09-10 2017-03-16 Life Technologies Corporation Purification of nucleic acid from environmental or biological samples
CN113832141B (zh) * 2021-09-30 2024-03-08 苏州唯善生物科技有限公司 粪便人源基因组dna特异性纯化方法及试剂盒

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0189280A3 (en) * 1985-01-23 1987-01-07 Dekalb-Pfizer Genetics Qualitative testing for nucleic acid
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5334499A (en) * 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
WO1993020235A1 (en) * 1992-04-01 1993-10-14 The Johns Hopkins University School Of Medicine Methods of detecting mammalian nucleic acids isolated from stool specimen and reagents therefor
EP0574267A3 (en) * 1992-06-12 1994-08-24 Gen Probe Inc Preparation of nucleic acid from blood

Also Published As

Publication number Publication date
DE19530132C2 (de) 1998-07-16
JP4264133B2 (ja) 2009-05-13
AU6821696A (en) 1997-03-12
EP0851937B1 (de) 2002-04-03
WO1997007239A1 (de) 1997-02-27
CA2228769C (en) 2011-04-12
DK0851937T3 (da) 2002-06-24
CA2228769A1 (en) 1997-02-27
EP0851937A1 (de) 1998-07-08
AU712331B2 (en) 1999-11-04
DE19530132A1 (de) 1997-02-20
DE59609027D1 (de) 2002-05-08
JPH11511020A (ja) 1999-09-28
US6084091A (en) 2000-07-04

Similar Documents

Publication Publication Date Title
DE59609027D1 (de) Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien
DE50015677D1 (de) Methode zur isolierung von dna aus biologischen materialien
DE69423249D1 (de) Verfahren und zusammensetzung zur verstärkten biologischen reinigung von erdöl
ATE186900T1 (de) Verfahren zur aeroben, weitergehenden biologischen reinigung von abwässern
DE69936946D1 (de) Reinigung von Antikörpern durch Ionenaustauschchromatographie
DE69331665D1 (de) Methode zur behandlung von nukleinsäureproben
DE59404477D1 (de) Verfahren zur abtrennung und reinigung von milchsäure
ATE242780T1 (de) Verfahren zur isolierung von nukleinsäure und vorrichtung hierfür
DE69815038D1 (de) Universales testsystem und verfahren zu seiner verwendung zur identifizierung diverse familien von mikroorganismen
DE59704304D1 (de) Verfahren und vorrichtung zur isolierung von nukleinsäuren
PL374311A1 (en) Methods for purifying protein
DE69406137D1 (de) Bioreaktor zur biologischen aufbereitung von verschmutztem wasser
DE69502313D1 (de) Verfahren zur biologischen Reinigung von Gasströmen
DE59603541D1 (de) Verfahren zur Reinigung von Bisphenol-A
DE69504381D1 (de) Verfahren zur Reinigung von Wasserstoffgas
DE69637579D1 (de) Methode zur Amplifizierung von Nukleinsäure und entsprechende Regenzien dafür
ATE247605T1 (de) Verfahren zum biologischen reinigen von abwasser
DE69801548D1 (de) Verfahren zur Reinigung von 1,4-Butandiol durch Schmelzkristallisation
ATE286487T1 (de) Verfahren zur reinigung von wasser, dafür geeignete bakterien und deren verwendung
DE69524164D1 (de) Verfahren zur Verhinderung des Absetzens von verschmutzenden Wasserorganismen
DE69616385D1 (de) Methode zur reinigung von prozessgasen
DE69833541D1 (de) Biologisches Verfahren und anoxischer und/oder aerobischer Bioreaktor zur Reinigung von flüssigen Abfällen
DE69800340D1 (de) Verfahren zur Reinigung von Naphthalin durch selektive Hydrierung und nachfolgende Trennung
DE69923778D1 (de) Verfahren und anlagen zum behandeln von abwässern aus kleinen gemeinschaften
DE59814359D1 (de) Mikrostrukturierter biosensor, verwendung des biosensors und verfahren zur immobilisierung von biokatalysatoren