CA2230263A1 - Peau reconstituee - Google Patents

Peau reconstituee Download PDF

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Publication number
CA2230263A1
CA2230263A1 CA002230263A CA2230263A CA2230263A1 CA 2230263 A1 CA2230263 A1 CA 2230263A1 CA 002230263 A CA002230263 A CA 002230263A CA 2230263 A CA2230263 A CA 2230263A CA 2230263 A1 CA2230263 A1 CA 2230263A1
Authority
CA
Canada
Prior art keywords
cells
dermis
dermal matrix
transplanted
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002230263A
Other languages
English (en)
Inventor
Stephen A. Livesey
Edward S. Griffey
Jerome Connor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LifeCell Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2230263A1 publication Critical patent/CA2230263A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C12N5/063—Kereatinocyte stem cells; Keratinocyte progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
    • C12N2533/92—Amnion; Decellularised dermis or mucosa

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Dermatology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Cette invention se rapporte à un procédé de production d'une peau composite comportant un derme inoculé directement avec des cellules mammaliennes. Selon ledit procédé, on obtient un derme qui est de préférence un derme acellulaire d'origine humaine ou xénogénique. Les cellules inoculées au derme sont transférées directement ou présélectionnées en fonction de caractéristiques compatibles avec celles d'une cellule souche ou d'une cellule parente. Ces cellules sont d'origine humaine ou xénogénique. Lors de la mise en pratique des réalisations préférées de cette invention, les cellules inoculées au derme ont la possibilité de se propager ex vivo dans une culture pour accroître leur nombre. Dans la réalisation préférée de l'invention, la culture ex vivo permet d'obtenir un accroissement de la population des cellules possédant des caractéristiques de type parent. Dans les réalisations préférées de cette invention, les cellules inoculées au derme sont modifiées génétiquement ex vivo. Dans la réalisation ayant la précellence, le derme à inoculer est d'origine humaine et les cellules sont soit autologues soit allogéniques, de par leur origine, ou une combinaison de ces deux types. Le derme d'origine humaine de cette réalisation préférée peut être utilisé sur l'homme ou servir ex vivo d'essai de laboratoire.
CA002230263A 1995-08-25 1996-08-22 Peau reconstituee Abandoned CA2230263A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US288295P 1995-08-25 1995-08-25
US60/002,882 1995-08-25

Publications (1)

Publication Number Publication Date
CA2230263A1 true CA2230263A1 (fr) 1997-03-06

Family

ID=21702998

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002230263A Abandoned CA2230263A1 (fr) 1995-08-25 1996-08-22 Peau reconstituee

Country Status (5)

Country Link
EP (1) EP0846162A1 (fr)
JP (1) JPH11511975A (fr)
AU (1) AU709010B2 (fr)
CA (1) CA2230263A1 (fr)
WO (1) WO1997008295A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1062322A2 (fr) * 1998-02-24 2000-12-27 Advanced Tissue Sciences, Inc. Matiere de reconstitution de peau chimere et vivante
WO1999064660A1 (fr) † 1998-06-10 1999-12-16 Kao Corporation Compositions d'adoucissants
AU2001284968B2 (en) 2000-08-16 2006-12-21 Duke University Decellularized tissue engineered constructs and tissues
US7262174B2 (en) 2001-05-09 2007-08-28 Geron Corporation Treatment for wounds
US8501396B2 (en) 2001-11-05 2013-08-06 Medgenics Medical Israel Ltd. Dermal micro-organs, methods and apparatuses for producing and using the same
US7468242B2 (en) 2001-11-05 2008-12-23 Medgenics, Inc. Dermal micro organs, methods and apparatuses for producing and using the same
US8088568B2 (en) 2001-11-05 2012-01-03 Medgentics, Inc. Dermal micro-organs, methods and apparatuses for producing and using the same
CA2466342A1 (fr) * 2001-11-09 2003-05-15 Es Cell International Pte Ltd Caracterisation et isolement de sous-ensembles de cellules souches embryonnaires humaines (hes) et de cellules associees a ces dernieres ou derivees de ces dernieres
EP2377403A1 (fr) * 2003-05-01 2011-10-19 Medgenics, Inc. Micro-organe dermique génétiquement modifié exprimant le facteur VIII
WO2008033375A2 (fr) 2006-09-14 2008-03-20 Medgenics Ltd. Formulations de médicament de longue durée
US8454948B2 (en) 2006-09-14 2013-06-04 Medgenics Medical Israel Ltd. Long lasting drug formulations
EP3473260B1 (fr) * 2007-07-10 2020-09-23 LifeCell Corporation Compositions de matrice tissulaire acellulaire pour la réparation tissulaire
JP2013532152A (ja) 2010-06-15 2013-08-15 メドジェニクス・メディカル・イスラエル・リミテッド 長期持続性の医薬製剤
US20170296697A1 (en) * 2014-10-03 2017-10-19 Cytori Therapeutics, Inc. Use of regenerative cells in mitigating burn progression and improving skin graft incorporation and healing

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4888291A (en) * 1987-06-19 1989-12-19 President And Fellows Of Harvard College Human epithelium originating from cell cultures
US5423778A (en) * 1989-12-14 1995-06-13 Elof Eriksson System and method for transplantation of cells
US5292655A (en) * 1990-01-29 1994-03-08 Wille Jr John J Method for the formation of a histologically-complete skin substitute
US5336616A (en) * 1990-09-12 1994-08-09 Lifecell Corporation Method for processing and preserving collagen-based tissues for transplantation

Also Published As

Publication number Publication date
EP0846162A1 (fr) 1998-06-10
AU709010B2 (en) 1999-08-19
AU6856896A (en) 1997-03-19
WO1997008295A1 (fr) 1997-03-06
JPH11511975A (ja) 1999-10-19

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