CA2268745A1 - Promoteurs extraits du lin permettant de manipuler l'expression genique - Google Patents

Promoteurs extraits du lin permettant de manipuler l'expression genique Download PDF

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Publication number
CA2268745A1
CA2268745A1 CA002268745A CA2268745A CA2268745A1 CA 2268745 A1 CA2268745 A1 CA 2268745A1 CA 002268745 A CA002268745 A CA 002268745A CA 2268745 A CA2268745 A CA 2268745A CA 2268745 A1 CA2268745 A1 CA 2268745A1
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CA
Canada
Prior art keywords
seq
sequence
plant
flax
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002268745A
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English (en)
Inventor
Ravinder Kumar Jain
Roberta Gail Thompson
Gordon Grant Rowland
Alan Gordon Mchughen
David Charles Taylor
Samuel Leonard Mackenzie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Council of Canada
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2268745A1 publication Critical patent/CA2268745A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C12N15/8226—Stem-specific, e.g. including tubers, beets
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention porte sur des séquences promoteurs obtenues à partir de lin et servant à modifier du lin ou d'autres plantes en vue de l'expression de gènes endogènes ou étrangers. Lesdits promoteurs sont des séquences SEQ ID NO 3 et SEQ ID NO 4 et proviennent de structures nouvellement découvertes de deux gènes SAD du lin, c.-à-d. les séquences SEQ ID NO 1 et SEQ ID NO 2. Les promoteurs ont été placés dans des plasmides de clonage et déposés à la "American Type Culture Collection" sous les appellations pCDC220 et pCDC214 sous les numéros respectifs de dépôt ATCC 98192 et ATCC 98193. Lesdits promoteurs peuvent être utilisés en conjonction avec des gènes pour modifier les caractéristiques du lin et d'autres plantes. L'invention inclut les gènes SAD eux-mêmes et des séquences d'ADN sensiblement homologues des SEQ ID NO 1, SEQ ID NO 2, SEQ ID NO 3 et SEQ ID NO 4, ainsi que leurs parties importantes.
CA002268745A 1996-10-31 1997-10-30 Promoteurs extraits du lin permettant de manipuler l'expression genique Abandoned CA2268745A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US2941696P 1996-10-31 1996-10-31
US60/029,416 1996-10-31
PCT/CA1997/000812 WO1998018948A1 (fr) 1996-10-31 1997-10-30 Promoteurs extraits du lin permettant de manipuler l'expression genique

Publications (1)

Publication Number Publication Date
CA2268745A1 true CA2268745A1 (fr) 1998-05-07

Family

ID=21848902

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002268745A Abandoned CA2268745A1 (fr) 1996-10-31 1997-10-30 Promoteurs extraits du lin permettant de manipuler l'expression genique

Country Status (4)

Country Link
AU (1) AU4858897A (fr)
CA (1) CA2268745A1 (fr)
GB (1) GB2332908B (fr)
WO (1) WO1998018948A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU782218B2 (en) * 1999-08-27 2005-07-14 Commonwealth Scientific And Industrial Research Organisation Flax seed specific promoters
US6777591B1 (en) * 1999-08-27 2004-08-17 Sembiosys Genetics Inc. Legume-like storage protein promoter isolated from flax and methods of expressing proteins in plant seeds using the promoter
US7642346B2 (en) * 1999-08-27 2010-01-05 Sembiosys Genetics Inc. Flax seed specific promoters
AU2002329522B2 (en) 2001-06-06 2008-05-22 Bioriginal Food & Science Corporation Flax (Linum usitatissimim L.) seed-specific promoters
EP1925671A1 (fr) * 2001-06-06 2008-05-28 Bioriginal Food & Science Corp. Promoteurs spécifiques à la graine lin (Linum usitatissimum L.)
EP1846560A4 (fr) * 2005-02-09 2009-02-11 Bioriginal Food & Science Corp Nouveaux membres de la famille de la desaturase d'acides gras omega-3 et leurs utilisations
CN102127562B (zh) 2009-12-09 2013-01-30 安胜军 一种种子特异型表达载体及其构建方法与应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE241007T1 (de) * 1990-03-16 2003-06-15 Calgene Llc Dnas, die für pflanzliche desaturasen kodieren und deren anwendungen
WO1991018985A1 (fr) * 1990-05-25 1991-12-12 E.I. Du Pont De Nemours And Company Sequence nucleotidique de gene de desaturase de stearoyle-acp de soja
US5475099A (en) * 1990-08-15 1995-12-12 Calgene Inc. Plant fatty acid synthases
NL9002130A (nl) * 1990-09-28 1992-04-16 Stichting Tech Wetenschapp Dna-sequentie die ten minste een deel van een gen coderend voor stearoyl-acp-desaturase omvat, alsmede toepassing van de dna-sequentie in een werkwijze voor het wijzigen van de vetzuurbiosynthese van een plant.
AU673994B2 (en) * 1992-03-13 1996-12-05 Agrigenetics, Inc. Modification of vegetable oil with desaturase
WO1995007357A2 (fr) * 1993-09-04 1995-03-16 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Promoteurs
BR9408435A (pt) * 1993-12-28 1997-08-05 Kirin Brewery Gene para dessaturase de ácido graxo vetor contendo o dito gene planta transformada com o dito gene e processo para criar a dita planta

Also Published As

Publication number Publication date
GB2332908B (en) 2001-03-28
GB9908939D0 (en) 1999-06-16
GB2332908A (en) 1999-07-07
AU4858897A (en) 1998-05-22
WO1998018948A1 (fr) 1998-05-07

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FZDE Discontinued