CA2308584A1 - Apport cible de gene dans un liposome vecteur - Google Patents

Apport cible de gene dans un liposome vecteur Download PDF

Info

Publication number
CA2308584A1
CA2308584A1 CA002308584A CA2308584A CA2308584A1 CA 2308584 A1 CA2308584 A1 CA 2308584A1 CA 002308584 A CA002308584 A CA 002308584A CA 2308584 A CA2308584 A CA 2308584A CA 2308584 A1 CA2308584 A1 CA 2308584A1
Authority
CA
Canada
Prior art keywords
liposome
nucleic acid
vector
complex
ligand
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002308584A
Other languages
English (en)
Other versions
CA2308584C (fr
Inventor
Esther H. Chang
Liang Xu
Kathleen Pirollo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Georgetown University
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CA2739604A priority Critical patent/CA2739604C/fr
Publication of CA2308584A1 publication Critical patent/CA2308584A1/fr
Application granted granted Critical
Publication of CA2308584C publication Critical patent/CA2308584C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers
    • A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers comprising non-phosphatidyl surfactants as bilayer-forming substances, e.g. cationic lipids or non-phosphatidyl liposomes coated or grafted with polymers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0008—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition
    • A61K48/0025—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition wherein the non-active part clearly interacts with the delivered nucleic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Organic Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biotechnology (AREA)
  • Epidemiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Manufacturing Of Micro-Capsules (AREA)

Abstract

On décrit des complexes (vecteurs) ciblés constitués d'un ligand, d'un liposome et d'une molécule thérapeutique qui sont utilisés pour apporter de manière systémique la molécule thérapeutique à divers types de cellules cibles, y compris à des cellules cancéreuses telles que celles de l'épithélioma spinocellulaire de la tête et du cou, et des tumeurs du sein et de la prostate. Les ligands préférés, le folate et la transferrine, ciblent le complexe de liposome et facilitent la transfection du gène transitoire. L'apport systémique de complexes contenant de l'ADN codant le gène p53 du type sauvage à des hétérogreffes établies de souris a manifestement sensibilisé les tumeurs à la radiothérapie et à la chimiothérapie. La combinaison de la thérapie du gène p53 systémique et de la radiothérapie ou de la chimiothérapie classique a eu pour effet la régression totale des tumeurs et l'inhibition à long terme de la récurrence. Ce système d'apport à spécificité cellulaire a également été utilisé in vivo pour apporter avec succès, par administration intraveineuse, de petites molécules d'ADN (oligonucléotides) qui ont induit une chimiosensibilité et l'inhibition de la croissance des hétérogreffes. D'autres molécules thérapeutiques, y compris des virus non transformés, peuvent être encapsulées dans un complexe et ciblées selon l'invention.
CA2308584A 1997-11-19 1998-11-19 Apport cible de gene dans un liposome vecteur Expired - Fee Related CA2308584C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2739604A CA2739604C (fr) 1997-11-19 1998-11-19 Apport cible de gene dans un liposome vecteur

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US6618897P 1997-11-19 1997-11-19
US60/066,188 1997-11-19
US8317598P 1998-04-27 1998-04-27
US60/083,175 1998-04-27
PCT/US1998/024657 WO1999025320A1 (fr) 1997-11-19 1998-11-19 Apport cible de gene dans un liposome vecteur

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2739604A Division CA2739604C (fr) 1997-11-19 1998-11-19 Apport cible de gene dans un liposome vecteur

Publications (2)

Publication Number Publication Date
CA2308584A1 true CA2308584A1 (fr) 1999-05-27
CA2308584C CA2308584C (fr) 2011-07-19

Family

ID=26746455

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2308584A Expired - Fee Related CA2308584C (fr) 1997-11-19 1998-11-19 Apport cible de gene dans un liposome vecteur

Country Status (12)

Country Link
EP (2) EP1032369B1 (fr)
JP (2) JP2001522871A (fr)
CN (3) CN1191094C (fr)
AT (2) ATE459340T1 (fr)
AU (1) AU759164C (fr)
CA (1) CA2308584C (fr)
DE (2) DE69830249T2 (fr)
DK (1) DK1166775T3 (fr)
ES (1) ES2339421T3 (fr)
HK (1) HK1045641B (fr)
PT (1) PT1166775E (fr)
WO (1) WO1999025320A1 (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8617514B2 (en) 1999-02-22 2013-12-31 Georgetown University Tumor-targeted nanodelivery systems to improve early MRI detection of cancer
JP4799736B2 (ja) 1999-02-22 2011-10-26 ジョージタウン・ユニバーシティ 全身性遺伝子送達のための抗体フラグメント標的化イムノリポソーム
US9034329B2 (en) 1999-02-22 2015-05-19 Georgetown University Preparation of antibody or an antibody fragment-targeted immunoliposomes for systemic administration of therapeutic or diagnostic agents and uses thereof
US9034330B2 (en) 1999-02-22 2015-05-19 Georgetown University Preparation of antibody or an antibody fragment-targeted immunoliposomes for systemic administration of therapeutic or diagnostic agents and uses thereof
US7780882B2 (en) 1999-02-22 2010-08-24 Georgetown University Simplified and improved method for preparing an antibody or an antibody fragment targeted immunoliposome for systemic administration of a therapeutic or diagnostic agent
US7109165B2 (en) * 2001-05-18 2006-09-19 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
WO2004066908A2 (fr) * 2003-01-17 2004-08-12 Sanofi Pasteur Composition comprenant lipides cationiques et un polynucleotide ou un polpeptide et methode pour leur delivrance ciblee
JP2006517032A (ja) * 2003-01-28 2006-07-13 ジョージタウン・ユニバーシティ ある種の癌治療の有効性を評価するための方法
CN1327899C (zh) * 2003-05-10 2007-07-25 彭朝晖 腺病毒载体与p53基因的基因重组体的应用
DK1633327T3 (da) * 2003-06-04 2010-11-15 Univ Georgetown Fremgangsmåde til forbedring af stabilitet og opbevaringslevetid af liposomkomplekser
US8627821B2 (en) 2005-01-10 2014-01-14 Pulmatrix, Inc. Method and device for decreasing contamination
US7858356B2 (en) * 2005-11-24 2010-12-28 Genomidea, Inc. Mutant paramyxovirus and method for production thereof
CN102133404B (zh) * 2011-03-23 2013-01-16 成都诺恩生物科技有限公司 一种肿瘤靶向治疗药物载体、其制备方法及其应用
WO2013093891A1 (fr) 2011-12-22 2013-06-27 Nuvo Research Gmbh Compositions liposomales comprenant des chlorites ou chlorates
US20140120157A1 (en) 2012-09-19 2014-05-01 Georgetown University Targeted liposomes
EP2711000B1 (fr) 2012-09-19 2019-04-03 Georgetown University Liposomes ciblés
CA2901616A1 (fr) 2013-03-14 2014-10-02 Georgetown University Traitement apres l'exposition a un agent neurotoxique
CN109069644B (zh) 2015-10-08 2022-10-28 犹他大学研究基金会 用于预防或治疗癌症的方法和组合物
JP2020500020A (ja) 2016-11-14 2020-01-09 ノバルティス アーゲー 融合誘導性タンパク質ｍｉｎｉｏｎに関連する組成物、方法、および治療上の使用
US12274784B2 (en) 2018-10-12 2025-04-15 Synergene Therapeutics, Inc. Methods for reducing side effects of immunotherapy

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5635380A (en) * 1994-01-18 1997-06-03 Vanderbilt University Enhancement of nucleic acid transfer by coupling virus to nucleic acid via lipids
US5928944A (en) * 1994-02-04 1999-07-27 The United States Of America As Represented By The Department Of Health And Human Services Method of adenoviral-medicated cell transfection
HUT77263A (hu) * 1994-09-13 1998-03-02 Theratech Inc. Kémiai anyagok bejuttatása egy specifikus sejttípus sejtjeinek belsejébe
US20020051767A1 (en) * 1995-09-13 2002-05-02 Yawen L. Chiang Cancer treatment
JPH09110722A (ja) * 1995-10-20 1997-04-28 Toray Ind Inc 抗腫瘍活性物質の腫瘍細胞内導入用イムノリポソーム及びその調製法
AU2112697A (en) * 1996-02-09 1997-08-28 Pi-Wan Cheng Receptor ligand-facilitated delivery of biologically active molecules
JP3956402B2 (ja) * 1996-03-04 2007-08-08 日本油脂株式会社 アンホテリシンｂ運搬体

Also Published As

Publication number Publication date
HK1045641A1 (en) 2002-12-06
EP1166775B1 (fr) 2010-03-03
HK1035490A1 (en) 2001-11-30
AU1464299A (en) 1999-06-07
CN1191094C (zh) 2005-03-02
EP1032369B1 (fr) 2005-05-18
AU759164C (en) 2007-03-29
EP1032369A1 (fr) 2000-09-06
ATE459340T1 (de) 2010-03-15
PT1166775E (pt) 2010-04-16
ES2339421T3 (es) 2010-05-20
CN1616665A (zh) 2005-05-18
CA2308584C (fr) 2011-07-19
JP2010263920A (ja) 2010-11-25
WO1999025320A1 (fr) 1999-05-27
DE69830249D1 (de) 2005-06-23
DE69830249T2 (de) 2006-05-04
HK1078608A1 (en) 2006-03-17
CN1616665B (zh) 2010-10-06
DE69841536D1 (de) 2010-04-15
EP1166775A2 (fr) 2002-01-02
AU759164B2 (en) 2003-04-10
CN1904055A (zh) 2007-01-31
JP2001522871A (ja) 2001-11-20
DK1166775T3 (da) 2010-05-03
CN1286630A (zh) 2001-03-07
ATE295722T1 (de) 2005-06-15
EP1166775A3 (fr) 2003-06-25
HK1045641B (en) 2010-10-29

Similar Documents

Publication Publication Date Title
CA2308584A1 (fr) Apport cible de gene dans un liposome vecteur
Hattori et al. Folate-linked lipid-based nanoparticle for targeted gene delivery
Santana-Armas et al. Strategies for cancer gene-delivery improvement by non-viral vectors
Zhao et al. Tumor-selective targeted delivery of genes and antisense oligodeoxyribonucleotides via the folate receptor
Tam et al. Stabilized plasmid-lipid particles for systemic gene therapy
US6749863B1 (en) Targeted liposome gene delivery
Wareing et al. Cationic liposome mediated transgene expression in the guinea pig cochlea
Monck et al. Stabilized plasmid–lipid particles: pharmacokinetics and plasmid delivery to distal tumors following intravenous injection
US6372250B1 (en) Non-invasive gene targeting to the brain
Vasileva et al. Composition of lipid nanoparticles for targeted delivery: application to mRNA therapeutics
Ko et al. Cationic liposomes loaded with proapoptotic peptide D-(KLAKLAK) 2 and Bcl-2 antisense oligodeoxynucleotide G3139 for enhanced anticancer therapy
EP1033972B1 (fr) Systeme d'administration d'un medicament avec ciblage en deux temps
Chu et al. Lipid nanoparticles as the drug carrier for targeted therapy of hepatic disorders
US7262173B2 (en) Chemosensitizing with liposomes containing oligonucleotides
Wang et al. RETRACTED ARTICLE: Nanoformulation of Apolipoprotein E3-Tagged Liposomal Nanoparticles for the co-Delivery of KRAS-siRNA and Gemcitabine for Pancreatic Cancer Treatment: Wang and Zhang
Hegde et al. Insight into lipid-based nanoplatform-mediated drug and gene delivery in neuro-oncology and their clinical prospects
Bruckheimer et al. In vivo efficacy of folate-targeted lipid–protamine–DNA (LPD-PEG-Folate) complexes in an immunocompetent syngeneic model for breast adenocarcinoma
WO2023230711A1 (fr) Nanoparticule lipidique pour l'administration d'arn
Idani et al. Intra‐tumoral injection of doxorubicin (adriamycin) encapsulated in liposome inhibits tumor growth, prolongs survival time and is not associated with local or systemic side effects
Yang et al. Photoimmunotherapy biomimetic nanoplatform for lung cancer and brain metastasis
Lu et al. Enhancement of p53 gene transfer efficiency in hepatic tumor mediated by transferrin receptor through trans-arterial delivery
EP1131457B1 (fr) Systeme d'apport genique systemique de virus/ligands et de therapie genique
CA2739604C (fr) Apport cible de gene dans un liposome vecteur
Hattori Cancer gene delivery with folate receptor targeting lipid-based nanoparticle
Gregory Folate Receptor-Targeted Liposomes

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20181119