CA2361678A1 - Methode de modulation de la biosynthese de metabolite dans des cellules recombinees - Google Patents

Methode de modulation de la biosynthese de metabolite dans des cellules recombinees Download PDF

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Publication number
CA2361678A1
CA2361678A1 CA002361678A CA2361678A CA2361678A1 CA 2361678 A1 CA2361678 A1 CA 2361678A1 CA 002361678 A CA002361678 A CA 002361678A CA 2361678 A CA2361678 A CA 2361678A CA 2361678 A1 CA2361678 A1 CA 2361678A1
Authority
CA
Canada
Prior art keywords
plant
cell
expression
transcription factor
metabolite
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002361678A
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English (en)
Inventor
Johan Memelink
Cornelia Theodora Elisabeth Van Der Fits
Franciscus Leonardus Hendrikus Menke
Jan Willem Kijne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universiteit Leiden
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2361678A1 publication Critical patent/CA2361678A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne une méthode permettant de moduler dans une cellule l'expression d'au moins un gène intervenant dans la biosynthèse d'un métabolite ou d'un précurseur de ce dernier. Cette méthode consiste à insérer dans une cellule une séquence de nucléotides codant pour un facteur de transcription qui comprend un domaine de liaison d'ADN AP2, et/ou en modifiant l'expression d'une séquence de nucléotides codant pour un tel facteur de transcription déjà présent dans la cellule. Ladite méthode prouve son utilité dans l'amélioration de la biosynthèse de métabolites secondaires chez les plantes, tels que les alcaloïdes, dont les alcaloïdes indoliques de terpène. Cette invention concerne également une méthode permettant d'améliorer la tolérance au stress chez les plantes à l'aide de tels facteurs de transcription.
CA002361678A 1999-02-05 2000-02-07 Methode de modulation de la biosynthese de metabolite dans des cellules recombinees Abandoned CA2361678A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA199900158 1999-02-05
DKPA199900158 1999-02-05
US11938899P 1999-02-10 1999-02-10
US60/119,388 1999-02-10
PCT/NL2000/000075 WO2000046383A2 (fr) 1999-02-05 2000-02-07 Methode de modulation de la biosynthese de metabolite dans des cellules recombinees

Publications (1)

Publication Number Publication Date
CA2361678A1 true CA2361678A1 (fr) 2000-08-10

Family

ID=26063424

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002361678A Abandoned CA2361678A1 (fr) 1999-02-05 2000-02-07 Methode de modulation de la biosynthese de metabolite dans des cellules recombinees

Country Status (6)

Country Link
EP (1) EP1157119A1 (fr)
JP (1) JP2002535993A (fr)
AU (1) AU779162B2 (fr)
CA (1) CA2361678A1 (fr)
NZ (1) NZ513356A (fr)
WO (1) WO2000046383A2 (fr)

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Also Published As

Publication number Publication date
AU779162B2 (en) 2005-01-06
EP1157119A1 (fr) 2001-11-28
WO2000046383A2 (fr) 2000-08-10
NZ513356A (en) 2004-01-30
WO2000046383A8 (fr) 2001-11-01
JP2002535993A (ja) 2002-10-29
AU2580600A (en) 2000-08-25
WO2000046383A3 (fr) 2001-09-07

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