CA2369002A1 - Methods for purifying dna using immobilized capture probes - Google Patents
Methods for purifying dna using immobilized capture probes Download PDFInfo
- Publication number
- CA2369002A1 CA2369002A1 CA002369002A CA2369002A CA2369002A1 CA 2369002 A1 CA2369002 A1 CA 2369002A1 CA 002369002 A CA002369002 A CA 002369002A CA 2369002 A CA2369002 A CA 2369002A CA 2369002 A1 CA2369002 A1 CA 2369002A1
- Authority
- CA
- Canada
- Prior art keywords
- sequencing reaction
- capture probe
- primer extension
- gel
- electrophoretic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/405—Concentrating samples by adsorption or absorption
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1048—General features of the devices using the transfer device for another function
- G01N2035/1053—General features of the devices using the transfer device for another function for separating part of the liquid, e.g. filters, extraction phase
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25946799A | 1999-02-26 | 1999-02-26 | |
| US09/259,467 | 1999-02-26 | ||
| PCT/US2000/004939 WO2000050644A2 (en) | 1999-02-26 | 2000-02-25 | Methods for purifying dna using immobilized capture probes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2369002A1 true CA2369002A1 (en) | 2000-08-31 |
Family
ID=22985081
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002369002A Abandoned CA2369002A1 (en) | 1999-02-26 | 2000-02-25 | Methods for purifying dna using immobilized capture probes |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1163371A2 (de) |
| JP (1) | JP2002537778A (de) |
| AU (1) | AU3708300A (de) |
| CA (1) | CA2369002A1 (de) |
| WO (1) | WO2000050644A2 (de) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE335845T1 (de) | 1997-05-16 | 2006-09-15 | Exact Sciences Corp | Elektrophoretische analyse von molekülen mit immobilisierten sonden |
| WO1999022228A1 (en) * | 1997-10-24 | 1999-05-06 | Northeastern University | A multichannel microscale system for high throughput preparative separation with comprehensive collection and analysis |
| JP4117249B2 (ja) | 2001-10-15 | 2008-07-16 | バイオセプト インコーポレイテッド | マイクロウェルバイオチップ |
| JP2005110503A (ja) * | 2003-10-02 | 2005-04-28 | Arkray Inc | 核酸の精製方法及びデバイス |
| WO2005047881A2 (en) | 2003-11-05 | 2005-05-26 | Exact Sciences Corporation | Repetitive reversed-field affinity electrophoresis and uses therefor |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5639603A (en) * | 1991-09-18 | 1997-06-17 | Affymax Technologies N.V. | Synthesizing and screening molecular diversity |
| CA2105962A1 (en) * | 1992-09-18 | 1994-03-19 | Margaret Patricia Raybuck | Device and method for affinity separation |
| US5876918A (en) * | 1993-03-08 | 1999-03-02 | Hydros, Inc. | Aligned fiber diagnostic chromatography with positive and negative controls |
| US5560811A (en) * | 1995-03-21 | 1996-10-01 | Seurat Analytical Systems Incorporated | Capillary electrophoresis apparatus and method |
-
2000
- 2000-02-25 CA CA002369002A patent/CA2369002A1/en not_active Abandoned
- 2000-02-25 JP JP2000601207A patent/JP2002537778A/ja active Pending
- 2000-02-25 EP EP00915885A patent/EP1163371A2/de not_active Withdrawn
- 2000-02-25 AU AU37083/00A patent/AU3708300A/en not_active Abandoned
- 2000-02-25 WO PCT/US2000/004939 patent/WO2000050644A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2000050644A2 (en) | 2000-08-31 |
| AU3708300A (en) | 2000-09-14 |
| WO2000050644A3 (en) | 2001-04-05 |
| JP2002537778A (ja) | 2002-11-12 |
| EP1163371A2 (de) | 2001-12-19 |
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| JP2003508015A (ja) | アダプター分子を用いる標的分子の電気泳動分析 | |
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| Schneider et al. | Recent Advances in DNA Separations: Plasmid Purification, Rapid Electrophoresis, and Affinity-Based Recovery |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Dead |