CA2539299C - Methode pour introduire un gene dans une plante - Google Patents
Methode pour introduire un gene dans une plante Download PDFInfo
- Publication number
- CA2539299C CA2539299C CA2539299A CA2539299A CA2539299C CA 2539299 C CA2539299 C CA 2539299C CA 2539299 A CA2539299 A CA 2539299A CA 2539299 A CA2539299 A CA 2539299A CA 2539299 C CA2539299 C CA 2539299C
- Authority
- CA
- Canada
- Prior art keywords
- agrobacterium
- pressurization
- plant
- plant material
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- 235000007340 Hordeum vulgare Nutrition 0.000 description 3
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- 101100049353 Hypocrea virens (strain Gv29-8 / FGSC 10586) virC gene Proteins 0.000 description 3
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- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
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- GPRBEKHLDVQUJE-VINNURBNSA-N cefotaxime Chemical compound N([C@@H]1C(N2C(=C(COC(C)=O)CS[C@@H]21)C(O)=O)=O)C(=O)/C(=N/OC)C1=CSC(N)=N1 GPRBEKHLDVQUJE-VINNURBNSA-N 0.000 description 2
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- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 2
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- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 2
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- 229930027917 kanamycin Natural products 0.000 description 2
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- 229930182823 kanamycin A Natural products 0.000 description 2
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 2
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- JXCKZXHCJOVIAV-UHFFFAOYSA-N 6-[(5-bromo-4-chloro-1h-indol-3-yl)oxy]-3,4,5-trihydroxyoxane-2-carboxylic acid;cyclohexanamine Chemical compound [NH3+]C1CCCCC1.O1C(C([O-])=O)C(O)C(O)C(O)C1OC1=CNC2=CC=C(Br)C(Cl)=C12 JXCKZXHCJOVIAV-UHFFFAOYSA-N 0.000 description 1
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 1
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- 244000068988 Glycine max Species 0.000 description 1
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- UOZODPSAJZTQNH-UHFFFAOYSA-N Paromomycin II Natural products NC1C(O)C(O)C(CN)OC1OC1C(O)C(OC2C(C(N)CC(N)C2O)OC2C(C(O)C(O)C(CO)O2)N)OC1CO UOZODPSAJZTQNH-UHFFFAOYSA-N 0.000 description 1
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- 229960001914 paromomycin Drugs 0.000 description 1
- UOZODPSAJZTQNH-LSWIJEOBSA-N paromomycin Chemical compound N[C@@H]1[C@@H](O)[C@H](O)[C@H](CN)O[C@@H]1O[C@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](N)C[C@@H](N)[C@@H]2O)O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)O[C@@H]1CO UOZODPSAJZTQNH-LSWIJEOBSA-N 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
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- 239000005648 plant growth regulator Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
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- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 1
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- 238000012546 transfer Methods 0.000 description 1
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- 230000004222 uncontrolled growth Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4636—Oryza sp. [rice]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4684—Zea mays [maize]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
- A01H6/823—Nicotiana, e.g. tobacco
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Developmental Biology & Embryology (AREA)
- General Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Physiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
L'invention porte sur un procédé visant à introduire un gène dans une plante par l'intermédiaire d'une bactérie du genre Agrobacterium. Ce procédé se caractérise en ce qu'il consiste à traiter une plante sous pression (1) et à infecter ensuite la plante avec Agrobacterium (2).
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2003-293125 | 2003-08-13 | ||
| JP2003293125 | 2003-08-13 | ||
| PCT/JP2004/011237 WO2005017169A1 (fr) | 2003-08-13 | 2004-08-05 | Procede visant a introduire un gene dans une plante |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2539299A1 CA2539299A1 (fr) | 2005-02-24 |
| CA2539299C true CA2539299C (fr) | 2011-11-15 |
Family
ID=34190984
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2539299A Expired - Lifetime CA2539299C (fr) | 2003-08-13 | 2004-08-05 | Methode pour introduire un gene dans une plante |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7812222B2 (fr) |
| EP (1) | EP1662002B1 (fr) |
| JP (1) | JP4532413B2 (fr) |
| KR (1) | KR20060061354A (fr) |
| CN (1) | CN100552034C (fr) |
| AT (1) | ATE485381T1 (fr) |
| AU (1) | AU2004264463B2 (fr) |
| CA (1) | CA2539299C (fr) |
| DE (1) | DE602004029691D1 (fr) |
| WO (1) | WO2005017169A1 (fr) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008001414A1 (fr) | 2006-06-23 | 2008-01-03 | Japan Tobacco Inc. | Vecteur cosmide destiné à transformer une plante et son procédé d'utilisation |
| JPWO2008105509A1 (ja) | 2007-02-28 | 2010-06-03 | 日本たばこ産業株式会社 | 選抜工程を経ないアグロバクテリウム菌による形質転換植物の作成方法 |
| EP2135503B1 (fr) | 2007-02-28 | 2014-01-22 | Japan Tobacco, Inc. | PROCÉDÉ D'AMÉLIORATION DE L'EFFICACITÉ DE LA TRANSFORMATION DANS UNE PLANTE, COMPRENANT UNE ÉTAPE DE CO-CULTURE POUR METTRE EN CULTURE UN TISSU VÉGÉTAL DANS UN MILIEU DE CO-CULTURE COMPRENANT DE L'ACIDE 3,6-DICHLORO-o-ANISIQUE |
| JP2011120478A (ja) * | 2008-03-31 | 2011-06-23 | Japan Tobacco Inc | アグロバクテリウム菌による形質転換植物の作成方法 |
| US9544924B2 (en) * | 2008-11-25 | 2017-01-10 | Lantiq Beteiligungs-GmbH & Co. KG | Ad hoc communication protocol method and apparatus |
| AU2010278126B2 (en) | 2009-07-29 | 2015-01-22 | Kaneka Corporation | A method of gene introduction into triticum plant using agrobacterium, and a method of producing transformed triticum plant |
| BR112013001189A2 (pt) | 2010-07-16 | 2016-05-31 | Philip Morris Products Sa | métodos para produção de proteínas em plantas |
| JP2013212051A (ja) | 2010-07-29 | 2013-10-17 | Japan Tobacco Inc | アグロバクテリウム菌を用いた、オオムギ属植物へ遺伝子導入を行う方法およびオオムギ属植物の形質転換植物の作成方法 |
| KR20140034139A (ko) | 2011-01-17 | 2014-03-19 | 필립모리스 프로덕츠 에스.에이. | 식물에서의 단백질 발현 |
| DE102013020605A1 (de) | 2013-12-15 | 2015-06-18 | Kws Saat Ag | Selektionsmarker-freies rhizobiaceae-vermitteltes verfahren zur herstellung einer transgenen pflanze der gattung triticum |
| WO2017079676A1 (fr) * | 2015-11-04 | 2017-05-11 | Massachusetts Institute Of Technology | Plantes électroluminescentes nanobioniques |
| JP2019129705A (ja) * | 2016-03-31 | 2019-08-08 | 日本たばこ産業株式会社 | 植物に物質を導入する方法 |
| CN106754630B (zh) * | 2016-12-21 | 2019-10-01 | 农业部环境保护科研监测所 | 一种水稻悬浮单细胞培养基及水稻单细胞的制备方法 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ239977A (en) * | 1990-11-14 | 1993-08-26 | Pioneer Hi Bred Int | Transforming plants by the use of agrobacterium |
| DE69334225D1 (de) * | 1992-07-07 | 2008-07-31 | Japan Tobacco Inc | Verfahren zur transformation einer monokotyledon pflanze |
| EP0672752B1 (fr) | 1993-09-03 | 2004-05-26 | Japan Tobacco Inc. | Procede permettant de transformer une monocotyledone avec un scutellum d'embryon immature |
| JPH10117776A (ja) * | 1996-10-22 | 1998-05-12 | Japan Tobacco Inc | インディカイネの形質転換方法 |
| WO1999048355A1 (fr) | 1998-03-20 | 1999-09-30 | Forbio Limited | Procede de transformation |
| JP2002534129A (ja) * | 1999-01-14 | 2002-10-15 | モンサント テクノロジー エルエルシー | ダイズ形質転換方法 |
| WO2002012521A1 (fr) | 1999-06-04 | 2002-02-14 | Japan Tobacco Inc. | Procede permettant d'ameliorer l'efficacite du transfert de genes dans des cellules vegetales |
| JP4428757B2 (ja) * | 1999-06-04 | 2010-03-10 | 日本たばこ産業株式会社 | 植物細胞への遺伝子導入の効率を向上させる方法 |
| JP2000342255A (ja) | 1999-06-04 | 2000-12-12 | Japan Tobacco Inc | 植物細胞への遺伝子導入の効率を向上させる方法 |
| JP4424784B2 (ja) * | 1999-06-04 | 2010-03-03 | 日本たばこ産業株式会社 | 植物細胞への遺伝子導入の効率を向上させる方法 |
| US6492174B1 (en) * | 2000-06-19 | 2002-12-10 | Institute Of Paper Science & Technology | Methods of initiating embryogenic cultures in plants |
| ES2277592T3 (es) | 2000-08-03 | 2007-07-16 | Japan Tobacco Inc. | Metodo para estimular la eficacia de introduccion de genes en celulas vegetales. |
| EP1437409A4 (fr) | 2001-09-19 | 2005-02-16 | Japan Tobacco Inc | Procede pour conferer ou reguler la fertilite d'un cytoplasme a sterilite male de riz bt au moyen d'un gene de restauration de la fertilite, et procede d'estimation de la presence d'un gene de restauration de la fertilite |
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2004
- 2004-08-05 DE DE602004029691T patent/DE602004029691D1/de not_active Expired - Lifetime
- 2004-08-05 US US10/567,866 patent/US7812222B2/en active Active
- 2004-08-05 AU AU2004264463A patent/AU2004264463B2/en not_active Expired
- 2004-08-05 CA CA2539299A patent/CA2539299C/fr not_active Expired - Lifetime
- 2004-08-05 WO PCT/JP2004/011237 patent/WO2005017169A1/fr not_active Ceased
- 2004-08-05 CN CNB2004800298956A patent/CN100552034C/zh not_active Expired - Lifetime
- 2004-08-05 KR KR1020067002825A patent/KR20060061354A/ko not_active Withdrawn
- 2004-08-05 EP EP04771264A patent/EP1662002B1/fr not_active Expired - Lifetime
- 2004-08-05 JP JP2005513158A patent/JP4532413B2/ja not_active Expired - Lifetime
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Also Published As
| Publication number | Publication date |
|---|---|
| CA2539299A1 (fr) | 2005-02-24 |
| CN100552034C (zh) | 2009-10-21 |
| US20070136898A1 (en) | 2007-06-14 |
| JPWO2005017169A1 (ja) | 2007-10-04 |
| AU2004264463A1 (en) | 2005-02-24 |
| ATE485381T1 (de) | 2010-11-15 |
| DE602004029691D1 (de) | 2010-12-02 |
| KR20060061354A (ko) | 2006-06-07 |
| CN1867675A (zh) | 2006-11-22 |
| EP1662002B1 (fr) | 2010-10-20 |
| EP1662002A1 (fr) | 2006-05-31 |
| EP1662002A4 (fr) | 2007-01-31 |
| AU2004264463B2 (en) | 2009-05-28 |
| WO2005017169A1 (fr) | 2005-02-24 |
| US7812222B2 (en) | 2010-10-12 |
| JP4532413B2 (ja) | 2010-08-25 |
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