CA2720472C - Procede de criblage de cellules individuelles pour la production d'agents biologiquement actifs - Google Patents

Procede de criblage de cellules individuelles pour la production d'agents biologiquement actifs Download PDF

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Publication number
CA2720472C
CA2720472C CA2720472A CA2720472A CA2720472C CA 2720472 C CA2720472 C CA 2720472C CA 2720472 A CA2720472 A CA 2720472A CA 2720472 A CA2720472 A CA 2720472A CA 2720472 C CA2720472 C CA 2720472C
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cells
protein
wells
interest
antibodies
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CA2720472A1 (fr
Inventor
Chun-Nan Chen
James O. Bowlby
Richard Aleck Jorgensen
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Single Cell Technology Inc
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Single Cell Technology Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B20/00—Methods specially adapted for identifying library members
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
    • Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Cette invention porte d'une manière générale sur des procédés, des dispositifs et des coffrets pour cribler des cellules individuelles pour la production d'un ou plusieurs agents biologiquement actifs d'intérêt, tels qu'une protéine, un acide nucléique ou une protéine et l'acide nucléique codant pour celle-ci.
CA2720472A 2008-04-05 2009-04-03 Procede de criblage de cellules individuelles pour la production d'agents biologiquement actifs Active CA2720472C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US7298508P 2008-04-05 2008-04-05
US61/072,985 2008-04-05
US14157908P 2008-12-30 2008-12-30
US61/141,579 2008-12-30
PCT/US2009/002122 WO2009123762A2 (fr) 2008-04-05 2009-04-03 Procédé de criblage de cellules individuelles pour la production d'agents biologiquement actifs

Publications (2)

Publication Number Publication Date
CA2720472A1 CA2720472A1 (fr) 2009-10-08
CA2720472C true CA2720472C (fr) 2018-01-02

Family

ID=41135895

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2720472A Active CA2720472C (fr) 2008-04-05 2009-04-03 Procede de criblage de cellules individuelles pour la production d'agents biologiquement actifs

Country Status (9)

Country Link
US (4) US9328172B2 (fr)
EP (1) EP2260112A4 (fr)
JP (1) JP5749156B2 (fr)
KR (1) KR101679869B1 (fr)
CN (1) CN102046809A (fr)
AU (1) AU2009232353B2 (fr)
BR (1) BRPI0906544A2 (fr)
CA (1) CA2720472C (fr)
WO (3) WO2009123756A1 (fr)

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Also Published As

Publication number Publication date
US8309317B2 (en) 2012-11-13
US20160201054A1 (en) 2016-07-14
US9328172B2 (en) 2016-05-03
US9873871B2 (en) 2018-01-23
JP5749156B2 (ja) 2015-07-15
US20110190148A1 (en) 2011-08-04
KR101679869B1 (ko) 2016-11-25
WO2009123756A1 (fr) 2009-10-08
AU2009232353B2 (en) 2015-04-30
EP2260112A4 (fr) 2013-10-09
AU2009232353A1 (en) 2009-10-08
CA2720472A1 (fr) 2009-10-08
BRPI0906544A2 (pt) 2019-01-02
US20100035763A1 (en) 2010-02-11
WO2009123748A1 (fr) 2009-10-08
CN102046809A (zh) 2011-05-04
EP2260112A2 (fr) 2010-12-15
US20090280992A1 (en) 2009-11-12
WO2009123762A3 (fr) 2009-12-30
WO2009123762A2 (fr) 2009-10-08
US8309035B2 (en) 2012-11-13
JP2011516064A (ja) 2011-05-26
KR20100131517A (ko) 2010-12-15

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