CA2856143A1 - Dispositifs actionneurs de gouttelettes et procedes employant des perles magnetiques - Google Patents

Dispositifs actionneurs de gouttelettes et procedes employant des perles magnetiques Download PDF

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Publication number
CA2856143A1
CA2856143A1 CA2856143A CA2856143A CA2856143A1 CA 2856143 A1 CA2856143 A1 CA 2856143A1 CA 2856143 A CA2856143 A CA 2856143A CA 2856143 A CA2856143 A CA 2856143A CA 2856143 A1 CA2856143 A1 CA 2856143A1
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Canada
Prior art keywords
droplet
beads
magnet
magnetically responsive
droplets
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Granted
Application number
CA2856143A
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English (en)
Other versions
CA2856143C (fr
Inventor
Allen E. Eckhardt
Vamsee K. Pamula
Michael G. Pollack
Ramakrishna Sista
Vijay Srinivasan
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Advanced Liquid Logic Inc
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Advanced Liquid Logic Inc
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Publication of CA2856143A1 publication Critical patent/CA2856143A1/fr
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
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    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/01—Drops
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44786—Apparatus specially adapted therefor of the magneto-electrophoresis type
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
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    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
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    • B01L2200/14—Process control and prevention of errors
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    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0427—Electrowetting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • G—PHYSICS
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    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G01N2035/1046—Levitated, suspended drops
    • G—PHYSICS
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    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Dispersion Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Electrochemistry (AREA)
  • Fluid Mechanics (AREA)
  • Inorganic Chemistry (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Soft Magnetic Materials (AREA)
  • Micromachines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2856143A 2007-02-09 2008-02-11 Dispositifs actionneurs de gouttelettes et procedes employant des perles magnetiques Active CA2856143C (fr)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US90065307P 2007-02-09 2007-02-09
US60/900,653 2007-02-09
US96973607P 2007-09-04 2007-09-04
US60/969,736 2007-09-04
US98076207P 2007-10-17 2007-10-17
US98077207P 2007-10-17 2007-10-17
US60/980,772 2007-10-17
US60/980,762 2007-10-17
CA2712863A CA2712863C (fr) 2007-02-09 2008-02-11 Dispositifs actionneurs de gouttelettes et procedes employant des perles magnetiques

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2712863A Division CA2712863C (fr) 2007-02-09 2008-02-11 Dispositifs actionneurs de gouttelettes et procedes employant des perles magnetiques

Publications (2)

Publication Number Publication Date
CA2856143A1 true CA2856143A1 (fr) 2008-08-14
CA2856143C CA2856143C (fr) 2016-11-01

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ID=39495992

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CA2712863A Active CA2712863C (fr) 2007-02-09 2008-02-11 Dispositifs actionneurs de gouttelettes et procedes employant des perles magnetiques
CA2856143A Active CA2856143C (fr) 2007-02-09 2008-02-11 Dispositifs actionneurs de gouttelettes et procedes employant des perles magnetiques

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Application Number Title Priority Date Filing Date
CA2712863A Active CA2712863C (fr) 2007-02-09 2008-02-11 Dispositifs actionneurs de gouttelettes et procedes employant des perles magnetiques

Country Status (11)

Country Link
US (4) US9046514B2 (fr)
EP (4) EP2573562A3 (fr)
JP (2) JP5156762B2 (fr)
KR (2) KR101503510B1 (fr)
CN (1) CN101627308B (fr)
AU (1) AU2008212808B2 (fr)
BR (1) BRPI0806831B8 (fr)
CA (2) CA2712863C (fr)
DK (1) DK2111554T3 (fr)
ES (1) ES2423930T3 (fr)
WO (1) WO2008098236A2 (fr)

Families Citing this family (134)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1859330T3 (da) 2005-01-28 2012-10-15 Univ Duke Apparater og fremgangsmåder til håndtering af små dråber på et trykt kredsløbskort
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US8613889B2 (en) 2006-04-13 2013-12-24 Advanced Liquid Logic, Inc. Droplet-based washing
US8637317B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Method of washing beads
WO2010027894A2 (fr) * 2008-08-27 2010-03-11 Advanced Liquid Logic, Inc. Actionneurs de gouttelettes, fluides modifiés et procédés associés
US8637324B2 (en) * 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8658111B2 (en) * 2006-04-18 2014-02-25 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
US7439014B2 (en) * 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
WO2010042637A2 (fr) * 2008-10-07 2010-04-15 Advanced Liquid Logic, Inc. Incubation et lavage de billes sur un actionneur à gouttelettes
WO2009140671A2 (fr) * 2008-05-16 2009-11-19 Advanced Liquid Logic, Inc. Dispositifs et procédés actionneurs de gouttelettes pour manipuler des billes
US8470606B2 (en) 2006-04-18 2013-06-25 Duke University Manipulation of beads in droplets and methods for splitting droplets
WO2009140373A2 (fr) * 2008-05-13 2009-11-19 Advanced Liquid Logic, Inc. Procédés, systèmes et dispositifs associés à un positionneur de gouttelettes
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US8716015B2 (en) 2006-04-18 2014-05-06 Advanced Liquid Logic, Inc. Manipulation of cells on a droplet actuator
WO2009111769A2 (fr) 2008-03-07 2009-09-11 Advanced Liquid Logic, Inc. Réactif et préparation et chargement d’un échantillon sur un dispositif fluidique
WO2008091848A2 (fr) 2007-01-22 2008-07-31 Advanced Liquid Logic, Inc. Chargement de fluide assisté en surface et distribution de gouttelette
ES2423930T3 (es) 2007-02-09 2013-09-25 Advanced Liquid Logic, Inc. Dispositivos actuadores de gotitas y métodos que emplean perlas magnéticas
WO2008101194A2 (fr) 2007-02-15 2008-08-21 Advanced Liquid Logic, Inc. Détection de capacité sur un actuateur goutte
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BRPI0806831A2 (pt) 2014-05-13
JP5833521B2 (ja) 2015-12-16
AU2008212808B2 (en) 2013-09-12
EP2111554B1 (fr) 2013-05-08
ES2423930T3 (es) 2013-09-25
BRPI0806831B8 (pt) 2021-07-27
EP2570812A2 (fr) 2013-03-20
KR20100014917A (ko) 2010-02-11
EP2570812A3 (fr) 2013-12-11
CA2856143C (fr) 2016-11-01
CA2712863A1 (fr) 2008-08-14
AU2008212808A1 (en) 2008-08-14
US20150314293A1 (en) 2015-11-05
KR20140078763A (ko) 2014-06-25
JP5156762B2 (ja) 2013-03-06
WO2008098236A3 (fr) 2009-01-15
US20250155430A1 (en) 2025-05-15
KR101431778B1 (ko) 2014-08-20
US12181467B2 (en) 2024-12-31
EP2573562A3 (fr) 2013-10-30
BRPI0806831B1 (pt) 2019-06-25
CN101627308B (zh) 2013-08-14
US9046514B2 (en) 2015-06-02
US20190324026A1 (en) 2019-10-24
EP2570812B1 (fr) 2018-07-18
EP2570811A2 (fr) 2013-03-20
EP2573562A2 (fr) 2013-03-27
US10379112B2 (en) 2019-08-13
JP2010518403A (ja) 2010-05-27
CN101627308A (zh) 2010-01-13
DK2111554T3 (da) 2013-07-22
JP2013029524A (ja) 2013-02-07
US20100068764A1 (en) 2010-03-18
WO2008098236A2 (fr) 2008-08-14
EP2111554A2 (fr) 2009-10-28
KR101503510B1 (ko) 2015-03-18
EP2570811A3 (fr) 2013-12-11
CA2712863C (fr) 2015-01-06
EP2570811B1 (fr) 2014-11-26

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