CH202461A - Process for the production of an insulin preparation. - Google Patents
Process for the production of an insulin preparation.Info
- Publication number
- CH202461A CH202461A CH202461DA CH202461A CH 202461 A CH202461 A CH 202461A CH 202461D A CH202461D A CH 202461DA CH 202461 A CH202461 A CH 202461A
- Authority
- CH
- Switzerland
- Prior art keywords
- insulin
- production
- reaction
- insulin preparation
- solution
- Prior art date
Links
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title claims description 22
- 102000004877 Insulin Human genes 0.000 title claims description 9
- 108090001061 Insulin Proteins 0.000 title claims description 9
- 229940125396 insulin Drugs 0.000 title claims description 9
- 238000002360 preparation method Methods 0.000 title claims description 6
- 238000000034 method Methods 0.000 title claims description 5
- 238000004519 manufacturing process Methods 0.000 title claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 239000003929 acidic solution Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 2
- 210000001124 body fluid Anatomy 0.000 claims 1
- 239000010839 body fluid Substances 0.000 claims 1
- 238000000354 decomposition reaction Methods 0.000 claims 1
- 210000002381 plasma Anatomy 0.000 claims 1
- 239000000243 solution Substances 0.000 description 5
- 102000007327 Protamines Human genes 0.000 description 3
- 108010007568 Protamines Proteins 0.000 description 3
- 229940048914 protamine Drugs 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000003113 alkalizing effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Endocrinology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Diabetes (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Description
Verfahren zur Herstellung eines Insulinpräparates. Gegenstarud, des vorliegenden Patentes, ist ein Verfahren zur Herstellung eines Insulin- präparates. Das Verfahren ist dadurch ge- kennzeichnet,
dass ein Insulinsalz und Cyclo- pterin in saurer Lösung miteinander in Re- aktion gebracht werden, wodurch Cyelopterin- insulinatgebildet wird.
Bei der D rchführung ,der Reaktion ist es vorteilhaft, die Gewichtsmenge -des Prot- amäns so zu bemessen, dass sie ,etwa 1/9 bis 1/l0 der Gewichtsmenge des Insulinsalzes beträgt.
Zu bemerken ist, @dass die Reaktion, wie sieh zeigte, bereits. bei gewöhnlicher Tem peratur stattfindet und sehr schnell vor sich geht. Dabei wird der saure Rest :des Insulin- salzes abgespalten.
Beispiel: 10 Gewichtsteile Insulin und 1,2 Ge- wichtsteile Cyclopterin werden in saurer Lö sung miteinander in Reaktion .gebracht. Der Säuregral .der Lösung wird grösser gewählt als einen. pH-Wert von aa. 4 entspricht,
und .die Reaktion wird bei gewöhnlicher Tem peratur ausgeführt. Infolge des genannten Säuregrades,der Lösung verbleibt,die bei der Reaktion ;gebildete neue chemische Verbin- ,dung (Cyclopterininsu:
linat) in Lösung, sie Iman aber durch Erhöhung des pH-Wertes ausgefällt werden. Das Produkt ist ein in Wasser- schwer lösliches, weisses und amor- phes Pulver, welches sich beim Erwärmen bei ca.
1,00 bis 120' C zu zersetzen anfängt.
Die gebildete chemische Verbindung Prot- amininsulinat ist im pH-Wertgebiet 7 bis 7,5 sehr Schwer löslich, während im Gegensatz hierzu beide l#eakti.onskomponenten bei diesen pH-Werten leicht löslich sind.
Die ganze Reaktionsmasse besteht nach Beendigung ,der rasch verlaufenden Reaktion aus einer Lösung von Protamininsulinat. Die ses Präparat wird zweckmässig in Ampullen eingefüllt,
die je einen einer gewissen Anzahl internationaler Insulineinheiten entsprechen den Inhalt aufnehmen. Die Lösung sollte nicht als solohe, sondern erst nach Fällung durch Zusatz eines alkalisierenden Stoffes be- nutzt werden.
Die durch Benutzung eines solehen In sulinpräparates entstandene Wirkung in dem menschlichen Organismus wird bedeutend langsamer verlaufen.
und infolgedessen auch bedeutend milder sein als es bei Verwendung von Insulinhydrochlorid der Fall ist., und ,dieser langsamere Wirkungsverlauf bewirkt zugleich, dass die Wiederholung einer Ein spritzung erst nach einer verhältnismässig langen Zeitperiode stattzufinden braucht.
Process for the production of an insulin preparation. Gegenstarud, the present patent, is a process for the production of an insulin preparation. The procedure is characterized by
that an insulin salt and cycloptherin are brought into reaction with one another in acidic solution, whereby cyelopterin insulinate is formed.
When carrying out the reaction, it is advantageous to measure the amount by weight of the protamine so that it is about 1/9 to 1/10 of the amount by weight of the insulin salt.
It should be noted @ that the reaction, as shown, is already. takes place at ordinary temperature and happens very quickly. The acidic residue of the insulin salt is split off.
Example: 10 parts by weight of insulin and 1.2 parts by weight of cyclopterine are brought into reaction with one another in an acidic solution. The acid level of the solution is chosen to be greater than one. pH of aa. 4 corresponds to
and .the reaction is carried out at ordinary temperature. As a result of the above-mentioned degree of acidity, the solution remains, the new chemical compound formed during the reaction (cyclopterininsu:
linat) in solution, but they are precipitated by increasing the pH value. The product is a white and amorphous powder that is sparingly soluble in water, which when heated at approx.
1.00 to 120 ° C begins to decompose.
The chemical compound formed, protamine insulinate, is very sparingly soluble in the pH range from 7 to 7.5, while in contrast to this, both action components are easily soluble at these pH values.
After the rapid reaction has ended, the entire reaction mass consists of a solution of protamine insulinate. This preparation is conveniently filled into ampoules,
which each correspond to a certain number of international insulin units take up the content. The solution should not be used on its own, but only after precipitation by adding an alkalizing substance.
The effect in the human organism resulting from the use of such an insulin preparation will proceed significantly more slowly.
and consequently also be significantly milder than is the case with the use of insulin hydrochloride., and, this slower course of action also means that the repetition of an injection only has to take place after a relatively long period of time.
Claims (1)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK202461X | 1935-04-08 | ||
| CH191962T | 1936-02-03 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CH202461A true CH202461A (en) | 1939-01-15 |
Family
ID=25722217
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CH202461D CH202461A (en) | 1935-04-08 | 1936-02-03 | Process for the production of an insulin preparation. |
Country Status (1)
| Country | Link |
|---|---|
| CH (1) | CH202461A (en) |
-
1936
- 1936-02-03 CH CH202461D patent/CH202461A/en unknown
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