CH498158A - Verfahren zur Anreicherung von L-Asparaginase - Google Patents

Verfahren zur Anreicherung von L-Asparaginase

Info

Publication number: CH498158A
Authority: CH; Switzerland
Prior art keywords: asparaginase; solution; enzyme; dependent; precipitation
Prior art date: 1967-12-27

Application number

CH1829568A

Other languages

German (de)

English (en)

Inventor

Otto Dr Wagner

Klaus Dr Bauer

Wilfried Dr Kaufmann

Erich Dr Rauenbusch

Alfred Dr Arens

Eckart Dr Irion

Original Assignee

Bayer Ag

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1967-12-27

Filing date

1968-12-05

Publication date

1970-10-31

1967-12-27 Priority claimed from DE19671642615 external-priority patent/DE1642615C3/de

1968-04-06 Priority claimed from DE19681767158 external-priority patent/DE1767158A1/de

1968-04-06 Priority claimed from DE19681767157 external-priority patent/DE1767157A1/de

1968-05-06 Priority claimed from DE19681767389 external-priority patent/DE1767389A1/de

1968-07-16 Priority claimed from DE19681792043 external-priority patent/DE1792043A1/de

1968-11-09 Priority claimed from DE19681808042 external-priority patent/DE1808042A1/de

1968-12-05 Application filed by Bayer Ag filed Critical Bayer Ag

1970-10-31 Publication of CH498158A publication Critical patent/CH498158A/de

Links

108010024976 Asparaginase Proteins 0.000 title claims abstract description 98
102100025573 1-alkyl-2-acetylglycerophosphocholine esterase Human genes 0.000 title claims abstract description 96
238000000034 method Methods 0.000 title claims abstract description 80
239000002510 pyrogen Substances 0.000 title claims abstract description 19
239000002202 Polyethylene glycol Substances 0.000 claims abstract description 56
229920001223 polyethylene glycol Polymers 0.000 claims abstract description 56
102000004190 Enzymes Human genes 0.000 claims abstract description 44
108090000790 Enzymes Proteins 0.000 claims abstract description 44
229940088598 enzyme Drugs 0.000 claims abstract description 44
230000000694 effects Effects 0.000 claims abstract description 29
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 28
239000000872 buffer Substances 0.000 claims abstract description 22
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 21
239000004471 Glycine Substances 0.000 claims abstract description 14
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims abstract description 3
BDAGIHXWWSANSR-UHFFFAOYSA-M Formate Chemical compound [O-]C=O BDAGIHXWWSANSR-UHFFFAOYSA-M 0.000 claims abstract description 3
239000000243 solution Substances 0.000 claims description 98
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 43
238000001556 precipitation Methods 0.000 claims description 34
108090000623 proteins and genes Proteins 0.000 claims description 27
102000004169 proteins and genes Human genes 0.000 claims description 26
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
239000002244 precipitate Substances 0.000 claims description 21
239000005695 Ammonium acetate Substances 0.000 claims description 20
229940043376 ammonium acetate Drugs 0.000 claims description 20
USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 claims description 18
235000019257 ammonium acetate Nutrition 0.000 claims description 18
239000000126 substance Substances 0.000 claims description 16
238000002360 preparation method Methods 0.000 claims description 14
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 12
239000004202 carbamide Substances 0.000 claims description 12
239000007864 aqueous solution Substances 0.000 claims description 11
229920001515 polyalkylene glycol Polymers 0.000 claims description 11
239000002904 solvent Substances 0.000 claims description 9
150000001408 amides Chemical class 0.000 claims description 8
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 7
150000001413 amino acids Chemical class 0.000 claims description 7
150000003839 salts Chemical class 0.000 claims description 6
239000000725 suspension Substances 0.000 claims description 6
239000001257 hydrogen Substances 0.000 claims description 5
229910052739 hydrogen Inorganic materials 0.000 claims description 5
DLFVBJFMPXGRIB-UHFFFAOYSA-N Acetamide Chemical compound CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 claims description 4
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 claims description 4
238000004587 chromatography analysis Methods 0.000 claims description 4
239000000470 constituent Substances 0.000 claims description 4
238000004108 freeze drying Methods 0.000 claims description 4
DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 claims description 3
239000012045 crude solution Substances 0.000 claims description 3
238000000502 dialysis Methods 0.000 claims description 3
239000003960 organic solvent Substances 0.000 claims description 3
150000001298 alcohols Chemical class 0.000 claims description 2
230000007935 neutral effect Effects 0.000 claims description 2
229920001451 polypropylene glycol Polymers 0.000 claims description 2
230000001419 dependent effect Effects 0.000 claims 14
229960000789 guanidine hydrochloride Drugs 0.000 claims 1
PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims 1
238000005194 fractionation Methods 0.000 abstract description 13
229920005654 Sephadex Polymers 0.000 abstract description 12
BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 abstract description 5
238000010828 elution Methods 0.000 abstract description 5
241000588724 Escherichia coli Species 0.000 abstract description 3
238000001914 filtration Methods 0.000 abstract description 2
230000000118 anti-neoplastic effect Effects 0.000 abstract 1
239000000427 antigen Substances 0.000 abstract 1
102000036639 antigens Human genes 0.000 abstract 1
108091007433 antigens Proteins 0.000 abstract 1
229940034982 antineoplastic agent Drugs 0.000 abstract 1
238000010438 heat treatment Methods 0.000 abstract 1
238000000746 purification Methods 0.000 abstract 1
230000000630 rising effect Effects 0.000 abstract 1
238000009210 therapy by ultrasound Methods 0.000 abstract 1
229960003272 asparaginase Drugs 0.000 description 21
DCXYFEDJOCDNAF-UHFFFAOYSA-M asparaginate Chemical compound [O-]C(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-M 0.000 description 21
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 15
QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 7
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 7
241001465754 Metazoa Species 0.000 description 7
239000013049 sediment Substances 0.000 description 7
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 6
241000283973 Oryctolagus cuniculus Species 0.000 description 5
239000012153 distilled water Substances 0.000 description 5
KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 4
238000005119 centrifugation Methods 0.000 description 4
235000019441 ethanol Nutrition 0.000 description 4
239000000499 gel Substances 0.000 description 4
238000011046 pyrogen test Methods 0.000 description 4
239000006228 supernatant Substances 0.000 description 4
239000010414 supernatant solution Substances 0.000 description 4
LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
229940024606 amino acid Drugs 0.000 description 3
229910021529 ammonia Inorganic materials 0.000 description 3
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 3
229910052921 ammonium sulfate Inorganic materials 0.000 description 3
235000011130 ammonium sulphate Nutrition 0.000 description 3
229960001230 asparagine Drugs 0.000 description 3
239000013078 crystal Substances 0.000 description 3
238000001035 drying Methods 0.000 description 3
239000012535 impurity Substances 0.000 description 3
238000004519 manufacturing process Methods 0.000 description 3
239000000463 material Substances 0.000 description 3
238000000926 separation method Methods 0.000 description 3
239000007858 starting material Substances 0.000 description 3
JVIPLYCGEZUBIO-UHFFFAOYSA-N 2-(4-fluorophenyl)-1,3-dioxoisoindole-5-carboxylic acid Chemical compound O=C1C2=CC(C(=O)O)=CC=C2C(=O)N1C1=CC=C(F)C=C1 JVIPLYCGEZUBIO-UHFFFAOYSA-N 0.000 description 2
241000894006 Bacteria Species 0.000 description 2
229920001425 Diethylaminoethyl cellulose Polymers 0.000 description 2
ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
241000209149 Zea Species 0.000 description 2
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
235000002017 Zea mays subsp mays Nutrition 0.000 description 2
VZTDIZULWFCMLS-UHFFFAOYSA-N ammonium formate Chemical compound [NH4+].[O-]C=O VZTDIZULWFCMLS-UHFFFAOYSA-N 0.000 description 2
230000001580 bacterial effect Effects 0.000 description 2
OHJMTUPIZMNBFR-UHFFFAOYSA-N biuret Chemical compound NC(=O)NC(N)=O OHJMTUPIZMNBFR-UHFFFAOYSA-N 0.000 description 2
238000004140 cleaning Methods 0.000 description 2
235000005822 corn Nutrition 0.000 description 2
238000002425 crystallisation Methods 0.000 description 2
230000008025 crystallization Effects 0.000 description 2
238000004925 denaturation Methods 0.000 description 2
230000036425 denaturation Effects 0.000 description 2
230000002255 enzymatic effect Effects 0.000 description 2
238000002474 experimental method Methods 0.000 description 2
238000005189 flocculation Methods 0.000 description 2
230000016615 flocculation Effects 0.000 description 2
239000000203 mixture Substances 0.000 description 2
235000015097 nutrients Nutrition 0.000 description 2
230000001376 precipitating effect Effects 0.000 description 2
239000000047 product Substances 0.000 description 2
230000001698 pyrogenic effect Effects 0.000 description 2
238000003756 stirring Methods 0.000 description 2
CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 description 1
208000004998 Abdominal Pain Diseases 0.000 description 1
208000002881 Colic Diseases 0.000 description 1
CKLJMWTZIZZHCS-UHFFFAOYSA-N D-OH-Asp Natural products OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 description 1
GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
229920002491 Diethylaminoethyl-dextran Polymers 0.000 description 1
ZAFNJMIOTHYJRJ-UHFFFAOYSA-N Diisopropyl ether Chemical compound CC(C)OC(C)C ZAFNJMIOTHYJRJ-UHFFFAOYSA-N 0.000 description 1
241000901842 Escherichia coli W Species 0.000 description 1
CKLJMWTZIZZHCS-UWTATZPHSA-N L-Aspartic acid Natural products OC(=O)[C@H](N)CC(O)=O CKLJMWTZIZZHCS-UWTATZPHSA-N 0.000 description 1
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 1
206010028980 Neoplasm Diseases 0.000 description 1
239000012507 Sephadex™ Substances 0.000 description 1
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
239000007983 Tris buffer Substances 0.000 description 1
238000003916 acid precipitation Methods 0.000 description 1
239000011149 active material Substances 0.000 description 1
WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
230000000890 antigenic effect Effects 0.000 description 1
239000012736 aqueous medium Substances 0.000 description 1
229960005261 aspartic acid Drugs 0.000 description 1
238000010923 batch production Methods 0.000 description 1
201000011510 cancer Diseases 0.000 description 1
AQWZKOAGNYYKLQ-UHFFFAOYSA-N carbamimidoylazanium;urea;chloride Chemical compound Cl.NC(N)=N.NC(N)=O AQWZKOAGNYYKLQ-UHFFFAOYSA-N 0.000 description 1
238000001311 chemical methods and process Methods 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
238000002512 chemotherapy Methods 0.000 description 1
238000004040 coloring Methods 0.000 description 1
238000004440 column chromatography Methods 0.000 description 1
239000013065 commercial product Substances 0.000 description 1
238000001816 cooling Methods 0.000 description 1
238000000354 decomposition reaction Methods 0.000 description 1
230000002542 deteriorative effect Effects 0.000 description 1
SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
239000012154 double-distilled water Substances 0.000 description 1
239000003814 drug Substances 0.000 description 1
239000003480 eluent Substances 0.000 description 1
238000000855 fermentation Methods 0.000 description 1
230000004151 fermentation Effects 0.000 description 1
238000005187 foaming Methods 0.000 description 1
229960004198 guanidine Drugs 0.000 description 1
229910052588 hydroxylapatite Inorganic materials 0.000 description 1
238000002329 infrared spectrum Methods 0.000 description 1
238000002347 injection Methods 0.000 description 1
239000007924 injection Substances 0.000 description 1
238000002955 isolation Methods 0.000 description 1
239000007788 liquid Substances 0.000 description 1
229910052943 magnesium sulfate Inorganic materials 0.000 description 1
235000019341 magnesium sulphate Nutrition 0.000 description 1
238000004452 microanalysis Methods 0.000 description 1
XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
229920000151 polyglycol Polymers 0.000 description 1
239000010695 polyglycol Substances 0.000 description 1
WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 1
239000002994 raw material Substances 0.000 description 1
239000012266 salt solution Substances 0.000 description 1
229920006395 saturated elastomer Polymers 0.000 description 1
238000004062 sedimentation Methods 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
150000004760 silicates Chemical class 0.000 description 1
238000005245 sintering Methods 0.000 description 1
239000001540 sodium lactate Substances 0.000 description 1
235000011088 sodium lactate Nutrition 0.000 description 1
229940005581 sodium lactate Drugs 0.000 description 1
239000007787 solid Substances 0.000 description 1
230000003381 solubilizing effect Effects 0.000 description 1
238000001179 sorption measurement Methods 0.000 description 1
230000000087 stabilizing effect Effects 0.000 description 1
239000000057 synthetic resin Substances 0.000 description 1
229920003002 synthetic resin Polymers 0.000 description 1
239000008399 tap water Substances 0.000 description 1
235000020679 tap water Nutrition 0.000 description 1
239000012085 test solution Substances 0.000 description 1
RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
238000002604 ultrasonography Methods 0.000 description 1
238000002211 ultraviolet spectrum Methods 0.000 description 1
239000005418 vegetable material Substances 0.000 description 1
238000005406 washing Methods 0.000 description 1
238000010626 work up procedure Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
- C12N9/82—Asparaginase (3.5.1.1)

Landscapes

Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Enzymes And Modification Thereof (AREA)
Knitting Of Fabric (AREA)
Knitting Machines (AREA)

CH1829568A 1967-12-27 1968-12-05 Verfahren zur Anreicherung von L-Asparaginase CH498158A (de)

Applications Claiming Priority (6)

Application Number	Priority Date	Filing Date	Title
DE19671642615 DE1642615C3 (de)	1967-12-27	1967-12-27	Verfahren zur Anreicherung von L-Asparaginase
DE19681767158 DE1767158A1 (de)	1968-04-06	1968-04-06	Verfahren zur Anreicherung von L-Asparaginase
DE19681767157 DE1767157A1 (de)	1968-04-06	1968-04-06	Verfahren zur Anreicherung von L-Asparaginase
DE19681767389 DE1767389A1 (de)	1968-05-06	1968-05-06	Verfahren zur Abtrennung von Pyrogenen aus Rohpraeparaten von L-Asparaginase
DE19681792043 DE1792043A1 (de)	1968-07-16	1968-07-16	Verfahren zur Herstellung pyrogenfreier L-Asparaginase
DE19681808042 DE1808042A1 (de)	1968-11-09	1968-11-09	Verfahren zur Herstellung pyrogenfreier L-Asparaginase

Publications (1)

Publication Number	Publication Date
CH498158A true CH498158A (de)	1970-10-31

Family

ID=27544076

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CH1829568A CH498158A (de)	1967-12-27	1968-12-05	Verfahren zur Anreicherung von L-Asparaginase

Country Status (10)

Country	Link
AT (1)	AT286212B (fr)
BE (1)	BE726113A (fr)
CH (1)	CH498158A (fr)
CS (1)	CS158632B2 (fr)
DK (1)	DK119351B (fr)
FI (1)	FI46073C (fr)
FR (2)	FR8410M (fr)
NL (1)	NL6818559A (fr)
NO (1)	NO127626B (fr)
SE (1)	SE366321B (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US3597323A (en) *	1968-06-07	1971-08-03	Wadley Res Inst & Blood Bank	Method of purifying l-asparaginase
FR2081581B1 (fr) *	1970-03-23	1973-08-10	Roussel Uclaf

1968
- 1968-12-05 CH CH1829568A patent/CH498158A/de not_active IP Right Cessation
- 1968-12-20 SE SE1765668A patent/SE366321B/xx unknown
- 1968-12-20 FI FI367168A patent/FI46073C/fi active
- 1968-12-23 NO NO515868A patent/NO127626B/no unknown
- 1968-12-23 AT AT1250468A patent/AT286212B/de not_active IP Right Cessation
- 1968-12-23 NL NL6818559A patent/NL6818559A/xx unknown
- 1968-12-23 DK DK632068A patent/DK119351B/da unknown
- 1968-12-27 BE BE726113D patent/BE726113A/xx unknown
- 1968-12-27 FR FR181244A patent/FR8410M/fr not_active Expired
- 1968-12-27 CS CS882168A patent/CS158632B2/cs unknown
- 1968-12-27 FR FR1598881D patent/FR1598881A/fr not_active Expired

Also Published As

Publication number	Publication date
NO127626B (fr)	1973-07-23
BE726113A (fr)	1969-05-29
AT286212B (de)	1970-11-25
FR1598881A (fr)	1970-07-06
FI46073B (fr)	1972-08-31
CS158632B2 (fr)	1974-11-25
SE366321B (fr)	1974-04-22
FI46073C (fi)	1972-12-11
NL6818559A (fr)	1969-07-01
DK119351B (da)	1970-12-21
FR8410M (fr)	1971-03-31

Legal Events

Date	Code	Title	Description
1977-07-15	PL	Patent ceased

Publication	Publication Date	Title
US3389133A (en)	1968-06-18	Process for isolating and purifying soluble ribonucleic acid
DE202015009775U1 (de)	2020-02-05	Abscheidung von 2'-FL aus einer Fermentationsbrühe
DD262673A5 (de)	1988-12-07	Verfahren zur extraktion von lipolhilen proteinen aus zellen der gattung pichia
DE2457090C2 (de)	1984-04-12	Verfahren zur Herstellung von Impfstoffen sowie Antibronchialimpfstoff
DE69315388T2 (de)	1998-05-14	Aus bakteriellen Proteinen bestehender Extrakt, Verfahren zur dessen Herstellung und diesen Extrakt enthaltende pharmazeutische Zusammensetzung
EP0065286B1 (fr)	1986-10-08	Procédé pour la purification ou l'enrichissement de protéines biologiquement actives et moyen approprié à cet effet
DE3806423A1 (de)	1988-09-08	Verfahren zur herstellung von pectin
DE2701890A1 (de)	1978-07-27	Alpha-amylase-inhibitor aus einem streptomyceten und verfahren zu seiner gewinnung
CH498158A (de)	1970-10-31	Verfahren zur Anreicherung von L-Asparaginase
DE2428955C3 (de)	1979-04-05	Verfahren zur Abtrennung der enzymatischen Komponente mit in vivo antikoagulierender und in vitro koagulierender Wirkung aus dem Gift der Schlange Ancistrodon rhodostoma und daraus hergestellte Mittel
DE1966427C3 (de)	1980-03-13	Verfahren zur Herstellung von 6-AminopenicUlansäure
DE1927517A1 (de)	1969-12-11	Verfahren zur Gewinnung und Reinigung von L-Asparaginase
DE2056294A1 (de)	1971-07-15	Verfahren zur Herstellung antiviraler zweistrangiger Ribonucleinsäuren
DE2304780A1 (de)	1973-08-09	Plasminostreptin und verfahren zu seiner herstellung
US3089816A (en)	1963-05-14	Lemacidine and process for its manufacture
DD222632A5 (de)	1985-05-22	Verfahren zur immobilisierung von carboxypeptidase-b-enzym
DE1642615C3 (de)	1977-12-22	Verfahren zur Anreicherung von L-Asparaginase
DE2907190A1 (de)	1980-08-28	1-desoxynojirimycin-herstellung
EP0133308B1 (fr)	1988-02-17	Agent pour le contrôle de l'appétit et son procédé de préparation
DE2154557A1 (de)	1973-05-10	Verfahren zur reinigung und kristallisation von kaudinogease (kallikrein)
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AT225846B (de)	1963-02-11	Verfahren zur Gewinnung reiner, pyrogenfreier Streptokinase