CH633258A5 - Process for preparing thymosin alpha 1 - Google Patents

Process for preparing thymosin alpha 1 Download PDF

Info

Publication number: CH633258A5
Authority: CH; Switzerland
Prior art keywords: buffer; thymus; thymosin; fraction; column
Prior art date: 1976-10-28

Application number

CH1216177A

Other languages

German (de)

English (en)

Inventor

Allan L Goldstein

Teresa L K Low

Chun-Yen Lai

Su-Sun Wang

Original Assignee

Hoffmann La Roche

Univ Texas

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1976-10-28

Filing date

1977-10-05

Publication date

1982-11-30

1977-02-08 Priority claimed from US05/766,638 external-priority patent/US4079127A/en

1977-10-05 Application filed by Hoffmann La Roche, Univ Texas filed Critical Hoffmann La Roche

1982-11-30 Publication of CH633258A5 publication Critical patent/CH633258A5/de

Links

238000004519 manufacturing process Methods 0.000 title description 2
108010078233 Thymalfasin Proteins 0.000 title 1
102400000800 Thymosin alpha-1 Human genes 0.000 title 1
NZVYCXVTEHPMHE-ZSUJOUNUSA-N thymalfasin Chemical compound CC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O NZVYCXVTEHPMHE-ZSUJOUNUSA-N 0.000 title 1
229960004231 thymalfasin Drugs 0.000 title 1
239000000872 buffer Substances 0.000 claims description 20
108010046075 Thymosin Proteins 0.000 claims description 14
102000007501 Thymosin Human genes 0.000 claims description 14
LCJVIYPJPCBWKS-NXPQJCNCSA-N thymosin Chemical compound SC[C@@H](N)C(=O)N[C@H](CO)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@H](C(C)C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](C(C)C)C(=O)N[C@H](CO)C(=O)N[C@H](CO)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H]([C@@H](C)CC)C(=O)N[C@H]([C@H](C)O)C(=O)N[C@H](C(C)C)C(=O)N[C@H](CCCCN)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CCCCN)C(=O)N[C@H](CCCCN)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](C(C)C)C(=O)N[C@H](C(C)C)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@H](CCC(O)=O)C(O)=O LCJVIYPJPCBWKS-NXPQJCNCSA-N 0.000 claims description 14
210000001541 thymus gland Anatomy 0.000 claims description 12
DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims description 12
238000000034 method Methods 0.000 claims description 10
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 9
108090000623 proteins and genes Proteins 0.000 claims description 9
102000004169 proteins and genes Human genes 0.000 claims description 9
229920005654 Sephadex Polymers 0.000 claims description 8
108700016958 thymosin fraction 5 Proteins 0.000 claims description 7
241000699670 Mus sp. Species 0.000 claims description 6
210000001744 T-lymphocyte Anatomy 0.000 claims description 5
239000007983 Tris buffer Substances 0.000 claims description 5
239000011780 sodium chloride Substances 0.000 claims description 5
239000000243 solution Substances 0.000 claims description 5
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 5
239000008223 sterile water Substances 0.000 claims description 4
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
-1 2-diethylaminoethyl Chemical group 0.000 claims description 3
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
230000024245 cell differentiation Effects 0.000 claims description 3
229960000789 guanidine hydrochloride Drugs 0.000 claims description 3
PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims description 3
239000000203 mixture Substances 0.000 claims description 3
238000002360 preparation method Methods 0.000 claims description 3
235000017281 sodium acetate Nutrition 0.000 claims description 3
239000001632 sodium acetate Substances 0.000 claims description 3
229920002134 Carboxymethyl cellulose Polymers 0.000 claims description 2
229920001425 Diethylaminoethyl cellulose Polymers 0.000 claims description 2
239000001768 carboxy methyl cellulose Substances 0.000 claims description 2
235000010948 carboxy methyl cellulose Nutrition 0.000 claims description 2
239000008112 carboxymethyl-cellulose Substances 0.000 claims description 2
230000003915 cell function Effects 0.000 claims description 2
229920001184 polypeptide Polymers 0.000 claims description 2
108090000765 processed proteins & peptides Proteins 0.000 claims description 2
102000004196 processed proteins & peptides Human genes 0.000 claims description 2
235000018102 proteins Nutrition 0.000 claims 4
206010028980 Neoplasm Diseases 0.000 claims 2
230000018109 developmental process Effects 0.000 claims 2
230000036737 immune function Effects 0.000 claims 2
210000000987 immune system Anatomy 0.000 claims 2
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims 1
102000011632 Caseins Human genes 0.000 claims 1
108010076119 Caseins Proteins 0.000 claims 1
241001465754 Metazoa Species 0.000 claims 1
208000031951 Primary immunodeficiency Diseases 0.000 claims 1
230000032683 aging Effects 0.000 claims 1
206010002022 amyloidosis Diseases 0.000 claims 1
238000004458 analytical method Methods 0.000 claims 1
230000001363 autoimmune Effects 0.000 claims 1
230000031018 biological processes and functions Effects 0.000 claims 1
201000011510 cancer Diseases 0.000 claims 1
239000005018 casein Substances 0.000 claims 1
BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims 1
235000021240 caseins Nutrition 0.000 claims 1
230000011748 cell maturation Effects 0.000 claims 1
238000006243 chemical reaction Methods 0.000 claims 1
230000001419 dependent effect Effects 0.000 claims 1
239000008062 guanidine hydrochloride buffer Substances 0.000 claims 1
230000007124 immune defense Effects 0.000 claims 1
230000002434 immunopotentiative effect Effects 0.000 claims 1
230000001771 impaired effect Effects 0.000 claims 1
230000007246 mechanism Effects 0.000 claims 1
230000008569 process Effects 0.000 claims 1
239000000724 thymus hormone Substances 0.000 claims 1
238000005406 washing Methods 0.000 claims 1
150000003839 salts Chemical class 0.000 description 5
239000002253 acid Substances 0.000 description 4
150000001413 amino acids Chemical group 0.000 description 3
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
239000012507 Sephadex™ Substances 0.000 description 2
230000000694 effects Effects 0.000 description 2
238000000338 in vitro Methods 0.000 description 2
238000002955 isolation Methods 0.000 description 2
239000003226 mitogen Substances 0.000 description 2
238000000746 purification Methods 0.000 description 2
WFIYPADYPQQLNN-UHFFFAOYSA-N 2-[2-(4-bromopyrazol-1-yl)ethyl]isoindole-1,3-dione Chemical compound C1=C(Br)C=NN1CCN1C(=O)C2=CC=CC=C2C1=O WFIYPADYPQQLNN-UHFFFAOYSA-N 0.000 description 1
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
108090000317 Chymotrypsin Proteins 0.000 description 1
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
CPELXLSAUQHCOX-UHFFFAOYSA-N Hydrogen bromide Chemical compound Br CPELXLSAUQHCOX-UHFFFAOYSA-N 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
102000009073 Macrophage Migration-Inhibitory Factors Human genes 0.000 description 1
108010048043 Macrophage Migration-Inhibitory Factors Proteins 0.000 description 1
241000124008 Mammalia Species 0.000 description 1
125000003047 N-acetyl group Chemical group 0.000 description 1
CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
102000007079 Peptide Fragments Human genes 0.000 description 1
108010033276 Peptide Fragments Proteins 0.000 description 1
108090000787 Subtilisin Proteins 0.000 description 1
QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
108090001109 Thermolysin Proteins 0.000 description 1
108090000631 Trypsin Proteins 0.000 description 1
102000004142 Trypsin Human genes 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
229940072107 ascorbate Drugs 0.000 description 1
235000010323 ascorbic acid Nutrition 0.000 description 1
239000011668 ascorbic acid Substances 0.000 description 1
WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 1
238000004166 bioassay Methods 0.000 description 1
230000004071 biological effect Effects 0.000 description 1
230000037396 body weight Effects 0.000 description 1
150000001720 carbohydrates Chemical class 0.000 description 1
235000014633 carbohydrates Nutrition 0.000 description 1
230000015556 catabolic process Effects 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
229960002376 chymotrypsin Drugs 0.000 description 1
238000006731 degradation reaction Methods 0.000 description 1
SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 1
201000010099 disease Diseases 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
230000007515 enzymatic degradation Effects 0.000 description 1
239000012634 fragment Substances 0.000 description 1
238000002523 gelfiltration Methods 0.000 description 1
229960004198 guanidine Drugs 0.000 description 1
238000001727 in vivo Methods 0.000 description 1
238000001990 intravenous administration Methods 0.000 description 1
238000004255 ion exchange chromatography Methods 0.000 description 1
238000001155 isoelectric focusing Methods 0.000 description 1
210000004698 lymphocyte Anatomy 0.000 description 1
229940049920 malate Drugs 0.000 description 1
VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
BJEPYKJPYRNKOW-UHFFFAOYSA-N malic acid Chemical compound OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
239000002773 nucleotide Substances 0.000 description 1
125000003729 nucleotide group Chemical group 0.000 description 1
150000007530 organic bases Chemical class 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
238000000926 separation method Methods 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
239000012588 trypsin Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/57581—Thymosin; Related peptides
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Biophysics (AREA)
Medicinal Chemistry (AREA)
Zoology (AREA)
Biochemistry (AREA)
Toxicology (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Gastroenterology & Hepatology (AREA)
Molecular Biology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Endocrinology (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Peptides Or Proteins (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)

CH1216177A 1976-10-28 1977-10-05 Process for preparing thymosin alpha 1 CH633258A5 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US73663876A	1976-10-28	1976-10-28
US05/766,638 US4079127A (en)	1976-10-28	1977-02-08	Thymosin alpha 1

Publications (1)

Publication Number	Publication Date
CH633258A5 true CH633258A5 (en)	1982-11-30

Family

ID=27113070

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CH1216177A CH633258A5 (en)	1976-10-28	1977-10-05	Process for preparing thymosin alpha 1

Country Status (22)

Country	Link
JP (1)	JPS5411220A (fr)
AR (1)	AR214903A1 (fr)
AT (1)	AT362493B (fr)
AU (1)	AU514996B2 (fr)
CA (1)	CA1101842A (fr)
CH (1)	CH633258A5 (fr)
DE (1)	DE2748213A1 (fr)
DK (1)	DK149094C (fr)
ES (1)	ES463588A1 (fr)
FI (1)	FI56317C (fr)
FR (1)	FR2369248A1 (fr)
GB (1)	GB1590457A (fr)
IL (1)	IL53218A (fr)
IT (1)	IT1195254B (fr)
LU (1)	LU78395A1 (fr)
MC (1)	MC1167A1 (fr)
NL (1)	NL188699C (fr)
NO (1)	NO143346C (fr)
NZ (1)	NZ185519A (fr)
PT (1)	PT67204B (fr)
SE (1)	SE442479B (fr)
YU (1)	YU40314B (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE2919592A1 (de) *	1979-05-15	1981-01-15	Max Planck Gesellschaft	Verfahren zur herstellung von thymosin- alpha 1 und derivaten davon
US4339427A (en) *	1980-04-14	1982-07-13	Hoffmann-La Roche Inc.	Radioimmunoassay of thymosinα
FR2492663B1 (fr) *	1980-10-24	1985-11-08	Vtoroi Mo G	Produit medicamenteux regulateur du t-systeme de l'immunite et son procede de preparation
DE3137231A1 (de) *	1981-09-18	1983-04-14	Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen	Bis-thymosin(alpha)(pfeil abwaerts)1(pfeil abwaerts)-verbindungen
JPH01250018A (ja) *	1988-03-30	1989-10-05	Noble Sangyo Kk	電子計測器用変位検知器

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB1195980A (en) *	1966-08-24	1970-06-24	Univ Yeshiva	Hormone-Like Preparations Derived from Thymus Gland and Methods of Producing the Same.

1977
- 1977-10-05 CH CH1216177A patent/CH633258A5/de not_active IP Right Cessation
- 1977-10-14 AU AU29699/77A patent/AU514996B2/en not_active Expired
- 1977-10-25 IL IL53218A patent/IL53218A/xx unknown
- 1977-10-26 MC MC771268A patent/MC1167A1/fr unknown
- 1977-10-26 CA CA289,510A patent/CA1101842A/fr not_active Expired
- 1977-10-26 FR FR7732259A patent/FR2369248A1/fr active Granted
- 1977-10-26 JP JP12766677A patent/JPS5411220A/ja active Granted
- 1977-10-26 IT IT29020/77A patent/IT1195254B/it active
- 1977-10-26 NZ NZ185519A patent/NZ185519A/xx unknown
- 1977-10-26 SE SE7712071A patent/SE442479B/xx not_active IP Right Cessation
- 1977-10-27 YU YU2578/77A patent/YU40314B/xx unknown
- 1977-10-27 AT AT765877A patent/AT362493B/de not_active IP Right Cessation
- 1977-10-27 AR AR269752A patent/AR214903A1/es active
- 1977-10-27 NL NLAANVRAGE7711814,A patent/NL188699C/xx not_active IP Right Cessation
- 1977-10-27 DE DE19772748213 patent/DE2748213A1/de active Granted
- 1977-10-27 NO NO773681A patent/NO143346C/no unknown
- 1977-10-27 DK DK478277A patent/DK149094C/da not_active IP Right Cessation
- 1977-10-27 LU LU7778395A patent/LU78395A1/xx unknown
- 1977-10-27 ES ES463588A patent/ES463588A1/es not_active Expired
- 1977-10-27 PT PT67204A patent/PT67204B/pt unknown
- 1977-10-28 FI FI773221A patent/FI56317C/fi not_active IP Right Cessation
- 1977-10-28 GB GB45001/77A patent/GB1590457A/en not_active Expired

Also Published As

Publication number	Publication date
LU78395A1 (fr)	1979-02-02
NZ185519A (en)	1980-08-26
NO773681L (no)	1978-05-02
FR2369248B1 (fr)	1981-01-02
SE7712071L (sv)	1978-04-29
DK149094B (da)	1986-01-20
AU2969977A (en)	1979-04-26
AR214903A1 (es)	1979-08-15
FI56317C (fi)	1980-01-10
DK478277A (da)	1978-04-29
DE2748213A1 (de)	1978-05-11
NL7711814A (nl)	1978-05-03
DK149094C (da)	1986-06-16
NL188699C (nl)	1992-09-01
YU40314B (en)	1985-12-31
AU514996B2 (en)	1981-03-12
JPS618805B2 (fr)	1986-03-18
CA1101842A (fr)	1981-05-26
GB1590457A (en)	1981-06-03
NL188699B (nl)	1992-04-01
ES463588A1 (es)	1978-12-16
SE442479B (sv)	1986-01-13
IL53218A0 (en)	1977-12-30
IT1195254B (it)	1988-10-12
NO143346B (no)	1980-10-13
IL53218A (en)	1981-03-31
JPS5411220A (en)	1979-01-27
ATA765877A (de)	1980-10-15
NO143346C (no)	1981-01-21
AT362493B (de)	1981-05-25
DE2748213C2 (fr)	1988-06-30
FI773221A7 (fi)	1978-04-29
PT67204A (en)	1977-11-01
MC1167A1 (fr)	1978-06-02
PT67204B (en)	1979-11-12
YU257877A (en)	1983-01-21
FR2369248A1 (fr)	1978-05-26
FI56317B (fi)	1979-09-28

Legal Events

Date	Code	Title	Description
1990-02-28	PUE	Assignment	Owner name: F. HOFFMANN-LA ROCHE AG
1997-10-31	PL	Patent ceased

Publication	Publication Date	Title
EP0167575B1 (fr)	1993-05-26	Cardiodilatine, une nouvelle hormone peptidique et son procede de fabrication
DE69224858T2 (de)	1998-07-23	Essentiell reines menschliches Parathyroidhormon
DE69418181T2 (de)	1999-12-23	Cytokine dämpfende substanzen
DE69108830T2 (de)	1995-09-14	Neue im schwein vorkommende physiologisch aktive peptide.
DE69029370T3 (de)	2006-02-23	Erythropoietin-Isoformen
DE69028671T2 (de)	1997-02-20	Löslisches extrazellulares Fragment des menschlischen IFN-beta 2/IL-6-Rezeptors, seine Herstellung und diesen Fragment enthaltende pharmazeutische Mischung
DE3586536T3 (de)	2002-05-08	Monoklonaler Antikörper gegen ein Cytotoxin, denselben erzeugendes Hybridoma und Verfahren für die Herstellung des gereinigten Cytotoxin.
Goldwasser et al.	1971	Purification of erythropoietin
DE69212011T2 (de)	1997-01-09	Endothelin Antagoniste
EP0021152B1 (fr)	1984-02-15	Procédé pour la détermination immunologique d'une matière de membrane basale, fragments de membranes basales spécifiques pour ce procédé et procédé pour leur préparation, resp. leur obtention
DE3650088T2 (de)	1995-01-26	Inhibin und dessen reinigung.
DE3935738A1 (de)	1991-05-08	Arzneimittel, enthaltend das humane parathormon-fragment (1-37) als aktiven wirkstoff
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