CH666048A5 - Composition-supplement pour un milieu de croissance de cellules, procede de sa preparation, composition-finale et utilisation. - Google Patents

Composition-supplement pour un milieu de croissance de cellules, procede de sa preparation, composition-finale et utilisation. Download PDF

Info

Publication number: CH666048A5
Authority: CH; Switzerland
Prior art keywords: supplement; serum; composition according; cells; composition
Prior art date: 1982-09-15

Application number

CH4970/83A

Other languages

English (en)

French (fr)

Inventor

Paul Russell Kelley

Orrie Max Friedman

Camille Josette Gaudreau

Gordon Hisashi Sato

Original Assignee

Collaborative Res Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1982-09-15

Filing date

1983-09-13

Publication date

1988-06-30

1983-09-13 Application filed by Collaborative Res Inc filed Critical Collaborative Res Inc

1988-06-30 Publication of CH666048A5 publication Critical patent/CH666048A5/fr

Links

239000000203 mixture Substances 0.000 title claims description 110
239000013589 supplement Substances 0.000 title claims description 81
230000010261 cell growth Effects 0.000 title claims description 21
239000001963 growth medium Substances 0.000 title claims description 21
238000000034 method Methods 0.000 title claims description 5
230000008569 process Effects 0.000 title description 3
210000002966 serum Anatomy 0.000 claims description 82
210000004027 cell Anatomy 0.000 claims description 51
241001465754 Metazoa Species 0.000 claims description 34
244000309466 calf Species 0.000 claims description 21
RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 claims description 18
MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 claims description 18
JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 claims description 18
SUHOOTKUPISOBE-UHFFFAOYSA-N O-phosphoethanolamine Chemical compound NCCOP(O)(O)=O SUHOOTKUPISOBE-UHFFFAOYSA-N 0.000 claims description 16
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 16
235000002639 sodium chloride Nutrition 0.000 claims description 15
230000012010 growth Effects 0.000 claims description 14
229940024606 amino acid Drugs 0.000 claims description 11
235000001014 amino acid Nutrition 0.000 claims description 11
150000001413 amino acids Chemical class 0.000 claims description 11
229910052500 inorganic mineral Inorganic materials 0.000 claims description 11
239000002609 medium Substances 0.000 claims description 11
235000010755 mineral Nutrition 0.000 claims description 11
239000011707 mineral Substances 0.000 claims description 11
150000003839 salts Chemical class 0.000 claims description 11
229940088594 vitamin Drugs 0.000 claims description 11
229930003231 vitamin Natural products 0.000 claims description 11
235000013343 vitamin Nutrition 0.000 claims description 11
239000011782 vitamin Substances 0.000 claims description 11
229910001868 water Inorganic materials 0.000 claims description 11
AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims description 10
OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 10
DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 claims description 10
102000004338 Transferrin Human genes 0.000 claims description 9
108090000901 Transferrin Proteins 0.000 claims description 9
229960005309 estradiol Drugs 0.000 claims description 9
229960000890 hydrocortisone Drugs 0.000 claims description 9
229960003387 progesterone Drugs 0.000 claims description 9
239000000186 progesterone Substances 0.000 claims description 9
229940000207 selenious acid Drugs 0.000 claims description 9
MCAHWIHFGHIESP-UHFFFAOYSA-N selenous acid Chemical compound O[Se](O)=O MCAHWIHFGHIESP-UHFFFAOYSA-N 0.000 claims description 9
229960003604 testosterone Drugs 0.000 claims description 9
239000012581 transferrin Substances 0.000 claims description 9
VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 claims description 8
102000009024 Epidermal Growth Factor Human genes 0.000 claims description 8
102000004877 Insulin Human genes 0.000 claims description 8
108090001061 Insulin Proteins 0.000 claims description 8
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims description 8
239000012888 bovine serum Substances 0.000 claims description 8
229930182833 estradiol Natural products 0.000 claims description 8
FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 claims description 8
229940125396 insulin Drugs 0.000 claims description 8
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
HNDVDQJCIGZPNO-YFKPBYRVSA-N L-Histidine Natural products OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims description 7
239000003102 growth factor Substances 0.000 claims description 7
-1 transport factors Substances 0.000 claims description 7
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 6
XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims description 6
238000000338 in vitro Methods 0.000 claims description 6
235000015097 nutrients Nutrition 0.000 claims description 6
KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical compound NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 claims description 6
WCDJCWGYYQTMGO-OAHLLOKOSA-N (2S)-2-amino-3-[4-(4-hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]-2,3,3-triiodopropanoic acid Chemical compound IC([C@](N)(C(=O)O)I)(C1=CC(I)=C(C(I)=C1)OC1=CC(I)=C(C(I)=C1)O)I WCDJCWGYYQTMGO-OAHLLOKOSA-N 0.000 claims description 5
239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims description 5
235000019743 Choline chloride Nutrition 0.000 claims description 5
AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims description 5
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims description 5
OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims description 5
DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims description 5
BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 claims description 5
239000003098 androgen Substances 0.000 claims description 5
229960003178 choline chloride Drugs 0.000 claims description 5
SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims description 5
238000011161 development Methods 0.000 claims description 5
239000012153 distilled water Substances 0.000 claims description 5
229940011871 estrogen Drugs 0.000 claims description 5
239000000262 estrogen Substances 0.000 claims description 5
229960000304 folic acid Drugs 0.000 claims description 5
239000011724 folic acid Substances 0.000 claims description 5
235000019152 folic acid Nutrition 0.000 claims description 5
229940088597 hormone Drugs 0.000 claims description 5
239000005556 hormone Substances 0.000 claims description 5
CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 5
229960003531 phenolsulfonphthalein Drugs 0.000 claims description 5
239000000047 product Substances 0.000 claims description 5
229960002477 riboflavin Drugs 0.000 claims description 5
239000002151 riboflavin Substances 0.000 claims description 5
235000019192 riboflavin Nutrition 0.000 claims description 5
CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 5
229940054269 sodium pyruvate Drugs 0.000 claims description 5
229960003495 thiamine Drugs 0.000 claims description 5
DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 5
235000019163 vitamin B12 Nutrition 0.000 claims description 5
239000011715 vitamin B12 Substances 0.000 claims description 5
GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 claims description 4
DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 claims description 4
UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 claims description 4
229930182844 L-isoleucine Natural products 0.000 claims description 4
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 4
UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 4
229930003779 Vitamin B12 Natural products 0.000 claims description 4
229940030486 androgens Drugs 0.000 claims description 4
IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 claims description 4
239000006143 cell culture medium Substances 0.000 claims description 4
AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 claims description 4
229910000397 disodium phosphate Inorganic materials 0.000 claims description 4
229960002885 histidine Drugs 0.000 claims description 4
229960000367 inositol Drugs 0.000 claims description 4
229960000310 isoleucine Drugs 0.000 claims description 4
235000019171 pyridoxine hydrochloride Nutrition 0.000 claims description 4
239000011764 pyridoxine hydrochloride Substances 0.000 claims description 4
229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 4
239000011780 sodium chloride Substances 0.000 claims description 4
229940104230 thymidine Drugs 0.000 claims description 4
229940011671 vitamin b6 Drugs 0.000 claims description 4
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
239000004201 L-cysteine Substances 0.000 claims description 3
235000013878 L-cysteine Nutrition 0.000 claims description 3
OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 claims description 3
101710098940 Pro-epidermal growth factor Proteins 0.000 claims description 3
239000005700 Putrescine Substances 0.000 claims description 3
210000004102 animal cell Anatomy 0.000 claims description 3
239000003246 corticosteroid Substances 0.000 claims description 3
239000008103 glucose Substances 0.000 claims description 3
OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 claims description 3
235000020778 linoleic acid Nutrition 0.000 claims description 3
229960003966 nicotinamide Drugs 0.000 claims description 3
235000005152 nicotinamide Nutrition 0.000 claims description 3
239000011570 nicotinamide Substances 0.000 claims description 3
LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 claims description 3
235000000346 sugar Nutrition 0.000 claims description 3
QDOVMBSZOOJLGR-ZBRNBAAYSA-N (2s)-2-amino-5-(diaminomethylideneamino)pentanoic acid;(2s)-2-aminopropanoic acid Chemical compound C[C@H](N)C(O)=O.OC(=O)[C@@H](N)CCCNC(N)=N QDOVMBSZOOJLGR-ZBRNBAAYSA-N 0.000 claims description 2
SITWEMZOJNKJCH-UHFFFAOYSA-N L-alanine-L-arginine Natural products CC(N)C(=O)NC(C(O)=O)CCCNC(N)=N SITWEMZOJNKJCH-UHFFFAOYSA-N 0.000 claims description 2
239000008346 aqueous phase Substances 0.000 claims description 2
229960003589 arginine hydrochloride Drugs 0.000 claims description 2
239000001110 calcium chloride Substances 0.000 claims description 2
235000011148 calcium chloride Nutrition 0.000 claims description 2
229910001628 calcium chloride Inorganic materials 0.000 claims description 2
229960001334 corticosteroids Drugs 0.000 claims description 2
229940068840 d-biotin Drugs 0.000 claims description 2
238000001914 filtration Methods 0.000 claims description 2
229910001629 magnesium chloride Inorganic materials 0.000 claims description 2
108090000765 processed proteins & peptides Proteins 0.000 claims description 2
230000002062 proliferating effect Effects 0.000 claims description 2
235000017557 sodium bicarbonate Nutrition 0.000 claims description 2
230000003381 solubilizing effect Effects 0.000 claims description 2
229910000368 zinc sulfate Inorganic materials 0.000 claims description 2
JVOFQCYNNDYCGN-HOXSEDPESA-N (2S)-2-amino-3-hydroxypropanoic acid (2S)-2-amino-4-methylsulfanylbutanoic acid (2S)-2-amino-3-phenylpropanoic acid (2S)-pyrrolidine-2-carboxylic acid Chemical compound OC[C@H](N)C(O)=O.OC(=O)[C@@H]1CCCN1.CSCC[C@H](N)C(O)=O.OC(=O)[C@@H](N)CC1=CC=CC=C1 JVOFQCYNNDYCGN-HOXSEDPESA-N 0.000 claims 1
YAZMMUXTWCLNOX-OYIBYFCASA-N (2S,3R)-2-amino-3-hydroxybutanoic acid (2S)-2-amino-3-(4-hydroxyphenyl)propanoic acid (2S)-2-amino-3-(1H-indol-3-yl)propanoic acid (2S)-2-amino-3-methylbutanoic acid Chemical compound CC(C)[C@H](N)C(O)=O.C[C@@H](O)[C@H](N)C(O)=O.OC(=O)[C@@H](N)CC1=CC=C(O)C=C1.C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 YAZMMUXTWCLNOX-OYIBYFCASA-N 0.000 claims 1
XIFDBACLRPLVEQ-BXRBKJIMSA-N (2s)-2-aminobutanedioic acid;(2s)-2,4-diamino-4-oxobutanoic acid Chemical compound OC(=O)[C@@H](N)CC(N)=O.OC(=O)[C@@H](N)CC(O)=O XIFDBACLRPLVEQ-BXRBKJIMSA-N 0.000 claims 1
ZEEYNQNRMIBLMK-DFWYDOINSA-N 2-aminoacetic acid;(2s)-2-aminopentanedioic acid Chemical compound NCC(O)=O.OC(=O)[C@@H](N)CCC(O)=O ZEEYNQNRMIBLMK-DFWYDOINSA-N 0.000 claims 1
229910000366 copper(II) sulfate Inorganic materials 0.000 claims 1
230000000050 nutritive effect Effects 0.000 claims 1
230000000153 supplemental effect Effects 0.000 claims 1
210000001685 thyroid gland Anatomy 0.000 claims 1
239000000306 component Substances 0.000 description 30
238000009472 formulation Methods 0.000 description 21
210000001519 tissue Anatomy 0.000 description 15
239000003104 tissue culture media Substances 0.000 description 13
239000000243 solution Substances 0.000 description 9
MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 8
AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 8
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 8
KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 8
239000000463 material Substances 0.000 description 7
ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 6
COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 6
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 6
210000002950 fibroblast Anatomy 0.000 description 6
238000004113 cell culture Methods 0.000 description 5
CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
FFEARJCKVFRZRR-UHFFFAOYSA-N L-Methionine Natural products CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 description 4
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 4
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 4
BVHLGVCQOALMSV-JEDNCBNOSA-N L-lysine hydrochloride Chemical compound Cl.NCCCC[C@H](N)C(O)=O BVHLGVCQOALMSV-JEDNCBNOSA-N 0.000 description 4
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 4
229930195722 L-methionine Natural products 0.000 description 4
239000004473 Threonine Substances 0.000 description 4
230000000694 effects Effects 0.000 description 4
229960004452 methionine Drugs 0.000 description 4
239000012071 phase Substances 0.000 description 4
235000018102 proteins Nutrition 0.000 description 4
102000004169 proteins and genes Human genes 0.000 description 4
108090000623 proteins and genes Proteins 0.000 description 4
229960001153 serine Drugs 0.000 description 4
229960002898 threonine Drugs 0.000 description 4
229960004441 tyrosine Drugs 0.000 description 4
229960004295 valine Drugs 0.000 description 4
KWTQSFXGGICVPE-WCCKRBBISA-N Arginine hydrochloride Chemical compound Cl.OC(=O)[C@@H](N)CCCN=C(N)N KWTQSFXGGICVPE-WCCKRBBISA-N 0.000 description 3
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 3
ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 3
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 3
229930182816 L-glutamine Natural products 0.000 description 3
239000004395 L-leucine Substances 0.000 description 3
235000019454 L-leucine Nutrition 0.000 description 3
229930182821 L-proline Natural products 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
239000011575 calcium Substances 0.000 description 3
230000007423 decrease Effects 0.000 description 3
230000018109 developmental process Effects 0.000 description 3
XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 3
229960003136 leucine Drugs 0.000 description 3
239000000813 peptide hormone Substances 0.000 description 3
229960005190 phenylalanine Drugs 0.000 description 3
229960002429 proline Drugs 0.000 description 3
230000008467 tissue growth Effects 0.000 description 3
229960004799 tryptophan Drugs 0.000 description 3
NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 2
ALYNCZNDIQEVRV-UHFFFAOYSA-N 4-aminobenzoic acid Chemical compound NC1=CC=C(C(O)=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-N 0.000 description 2
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
108091003079 Bovine Serum Albumin Proteins 0.000 description 2
206010006187 Breast cancer Diseases 0.000 description 2
241000282552 Chlorocebus aethiops Species 0.000 description 2
QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 description 2
QNAYBMKLOCPYGJ-UWTATZPHSA-N L-Alanine Natural products C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 description 2
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
206010035226 Plasma cell myeloma Diseases 0.000 description 2
208000024313 Testicular Neoplasms Diseases 0.000 description 2
241000219995 Wisteria Species 0.000 description 2
239000002253 acid Substances 0.000 description 2
239000000654 additive Substances 0.000 description 2
239000003570 air Substances 0.000 description 2
229960003767 alanine Drugs 0.000 description 2
229960001230 asparagine Drugs 0.000 description 2
230000008901 benefit Effects 0.000 description 2
239000000872 buffer Substances 0.000 description 2
229910002092 carbon dioxide Inorganic materials 0.000 description 2
239000001569 carbon dioxide Substances 0.000 description 2
239000000969 carrier Substances 0.000 description 2
230000001413 cellular effect Effects 0.000 description 2
230000003511 endothelial effect Effects 0.000 description 2
239000012894 fetal calf serum Substances 0.000 description 2
239000013020 final formulation Substances 0.000 description 2
239000012467 final product Substances 0.000 description 2
229960002989 glutamic acid Drugs 0.000 description 2
244000144980 herd Species 0.000 description 2
210000004408 hybridoma Anatomy 0.000 description 2
239000004615 ingredient Substances 0.000 description 2
229910000359 iron(II) sulfate Inorganic materials 0.000 description 2
210000003734 kidney Anatomy 0.000 description 2
210000001161 mammalian embryo Anatomy 0.000 description 2
238000004519 manufacturing process Methods 0.000 description 2
238000002156 mixing Methods 0.000 description 2
201000000050 myeloid neoplasm Diseases 0.000 description 2
208000025189 neoplasm of testis Diseases 0.000 description 2
FCHXJFJNDJXENQ-UHFFFAOYSA-N pyridoxal hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(C=O)=C1O FCHXJFJNDJXENQ-UHFFFAOYSA-N 0.000 description 2
238000011160 research Methods 0.000 description 2
230000007928 solubilization Effects 0.000 description 2
238000005063 solubilization Methods 0.000 description 2
239000011550 stock solution Substances 0.000 description 2
201000003120 testicular cancer Diseases 0.000 description 2
229940036555 thyroid hormone Drugs 0.000 description 2
239000005495 thyroid hormone Substances 0.000 description 2
DNXHEGUUPJUMQT-UHFFFAOYSA-N (+)-estrone Natural products OC1=CC=C2C3CCC(C)(C(CC4)=O)C4C3CCC2=C1 DNXHEGUUPJUMQT-UHFFFAOYSA-N 0.000 description 1
QZNNVYOVQUKYSC-JEDNCBNOSA-N (2s)-2-amino-3-(1h-imidazol-5-yl)propanoic acid;hydron;chloride Chemical compound Cl.OC(=O)[C@@H](N)CC1=CN=CN1 QZNNVYOVQUKYSC-JEDNCBNOSA-N 0.000 description 1
OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
QDGAVODICPCDMU-UHFFFAOYSA-N 2-amino-3-[3-[bis(2-chloroethyl)amino]phenyl]propanoic acid Chemical compound OC(=O)C(N)CC1=CC=CC(N(CCCl)CCCl)=C1 QDGAVODICPCDMU-UHFFFAOYSA-N 0.000 description 1
AYLDUGBOZJEHLW-UHFFFAOYSA-N 4,5-bis(hydroxymethyl)-2-methylpyridin-3-ol;pyridine-3-carboxamide Chemical compound NC(=O)C1=CC=CN=C1.CC1=NC=C(CO)C(CO)=C1O AYLDUGBOZJEHLW-UHFFFAOYSA-N 0.000 description 1
229920001817 Agar Polymers 0.000 description 1
QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
101001011741 Bos taurus Insulin Proteins 0.000 description 1
208000026310 Breast neoplasm Diseases 0.000 description 1
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
241000283707 Capra Species 0.000 description 1
201000009030 Carcinoma Diseases 0.000 description 1
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
102000008186 Collagen Human genes 0.000 description 1
108010035532 Collagen Proteins 0.000 description 1
RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
OMFXVFTZEKFJBZ-UHFFFAOYSA-N Corticosterone Natural products O=C1CCC2(C)C3C(O)CC(C)(C(CC4)C(=O)CO)C4C3CCC2=C1 OMFXVFTZEKFJBZ-UHFFFAOYSA-N 0.000 description 1
241000699800 Cricetinae Species 0.000 description 1
CKLJMWTZIZZHCS-UHFFFAOYSA-N D-OH-Asp Natural products OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 description 1
235000000638 D-biotin Nutrition 0.000 description 1
239000011665 D-biotin Substances 0.000 description 1
101800003838 Epidermal growth factor Proteins 0.000 description 1
DNXHEGUUPJUMQT-CBZIJGRNSA-N Estrone Chemical compound OC1=CC=C2[C@H]3CC[C@](C)(C(CC4)=O)[C@@H]4[C@@H]3CCC2=C1 DNXHEGUUPJUMQT-CBZIJGRNSA-N 0.000 description 1
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
108090000386 Fibroblast Growth Factor 1 Proteins 0.000 description 1
102100031706 Fibroblast growth factor 1 Human genes 0.000 description 1
241000287828 Gallus gallus Species 0.000 description 1
208000032612 Glial tumor Diseases 0.000 description 1
206010018338 Glioma Diseases 0.000 description 1
102000006395 Globulins Human genes 0.000 description 1
108010044091 Globulins Proteins 0.000 description 1
239000004471 Glycine Substances 0.000 description 1
102000018997 Growth Hormone Human genes 0.000 description 1
108010051696 Growth Hormone Proteins 0.000 description 1
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
CKLJMWTZIZZHCS-UWTATZPHSA-N L-Aspartic acid Natural products OC(=O)[C@H](N)CC(O)=O CKLJMWTZIZZHCS-UWTATZPHSA-N 0.000 description 1
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 1
239000004158 L-cystine Substances 0.000 description 1
235000019393 L-cystine Nutrition 0.000 description 1
FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
101150114843 Mgll gene Proteins 0.000 description 1
241001529936 Murinae Species 0.000 description 1
206010028980 Neoplasm Diseases 0.000 description 1
PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
241000283973 Oryctolagus cuniculus Species 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
241001494479 Pecora Species 0.000 description 1
ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
RMKOIUPDIRKDQF-NYIJDJKZSA-N SC[C@H](N)C(O)=O.OC(=O)[C@@H](N)CC(N)=O.OC(=O)[C@@H](N)CC(O)=O Chemical compound SC[C@H](N)C(O)=O.OC(=O)[C@@H](N)CC(N)=O.OC(=O)[C@@H](N)CC(O)=O RMKOIUPDIRKDQF-NYIJDJKZSA-N 0.000 description 1
206010039491 Sarcoma Diseases 0.000 description 1
208000000102 Squamous Cell Carcinoma of Head and Neck Diseases 0.000 description 1
QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
241000700605 Viruses Species 0.000 description 1
FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
MECHNRXZTMCUDQ-UHFFFAOYSA-N Vitamin D2 Natural products C1CCC2(C)C(C(C)C=CC(C)C(C)C)CCC2C1=CC=C1CC(O)CCC1=C MECHNRXZTMCUDQ-UHFFFAOYSA-N 0.000 description 1
DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 description 1
230000009471 action Effects 0.000 description 1
208000009956 adenocarcinoma Diseases 0.000 description 1
239000008272 agar Substances 0.000 description 1
238000013019 agitation Methods 0.000 description 1
FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
235000010323 ascorbic acid Nutrition 0.000 description 1
229960005070 ascorbic acid Drugs 0.000 description 1
239000011668 ascorbic acid Substances 0.000 description 1
229960005261 aspartic acid Drugs 0.000 description 1
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
235000015278 beef Nutrition 0.000 description 1
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
239000012620 biological material Substances 0.000 description 1
238000001574 biopsy Methods 0.000 description 1
229960002685 biotin Drugs 0.000 description 1
235000020958 biotin Nutrition 0.000 description 1
239000011616 biotin Substances 0.000 description 1
IXIBAKNTJSCKJM-BUBXBXGNSA-N bovine insulin Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)C(C)C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 IXIBAKNTJSCKJM-BUBXBXGNSA-N 0.000 description 1
201000008275 breast carcinoma Diseases 0.000 description 1
239000006227 byproduct Substances 0.000 description 1
229910052791 calcium Inorganic materials 0.000 description 1
229910002091 carbon monoxide Inorganic materials 0.000 description 1
230000011712 cell development Effects 0.000 description 1
208000019065 cervical carcinoma Diseases 0.000 description 1
229920001436 collagen Polymers 0.000 description 1
239000000470 constituent Substances 0.000 description 1
238000010924 continuous production Methods 0.000 description 1
229910052802 copper Inorganic materials 0.000 description 1
239000010949 copper Substances 0.000 description 1
OMFXVFTZEKFJBZ-HJTSIMOOSA-N corticosterone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@H](CC4)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 OMFXVFTZEKFJBZ-HJTSIMOOSA-N 0.000 description 1
150000001887 cortisones Chemical class 0.000 description 1
229960003067 cystine Drugs 0.000 description 1
229960003957 dexamethasone Drugs 0.000 description 1
UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 1
238000007865 diluting Methods 0.000 description 1
230000009088 enzymatic function Effects 0.000 description 1
229940116977 epidermal growth factor Drugs 0.000 description 1
229960002061 ergocalciferol Drugs 0.000 description 1
229960003399 estrone Drugs 0.000 description 1
230000006870 function Effects 0.000 description 1
239000007789 gas Substances 0.000 description 1
229960002743 glutamine Drugs 0.000 description 1
239000000122 growth hormone Substances 0.000 description 1
IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 1
238000011835 investigation Methods 0.000 description 1
229910052742 iron Inorganic materials 0.000 description 1
210000003292 kidney cell Anatomy 0.000 description 1
AGBQKNBQESQNJD-UHFFFAOYSA-M lipoate Chemical compound [O-]C(=O)CCCCC1CCSS1 AGBQKNBQESQNJD-UHFFFAOYSA-M 0.000 description 1
235000019136 lipoic acid Nutrition 0.000 description 1
239000011777 magnesium Substances 0.000 description 1
229910052749 magnesium Inorganic materials 0.000 description 1
WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
210000003205 muscle Anatomy 0.000 description 1
210000003098 myoblast Anatomy 0.000 description 1
229960004719 nandrolone Drugs 0.000 description 1
NPAGDVCDWIYMMC-IZPLOLCNSA-N nandrolone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 NPAGDVCDWIYMMC-IZPLOLCNSA-N 0.000 description 1
229960003512 nicotinic acid Drugs 0.000 description 1
235000001968 nicotinic acid Nutrition 0.000 description 1
239000011664 nicotinic acid Substances 0.000 description 1
229910052757 nitrogen Inorganic materials 0.000 description 1
230000008481 normal tissue growth Effects 0.000 description 1
201000002740 oral squamous cell carcinoma Diseases 0.000 description 1
239000001301 oxygen Substances 0.000 description 1
229910052760 oxygen Inorganic materials 0.000 description 1
ICMWWNHDUZJFDW-DHODBPELSA-N oxymetholone Chemical compound C([C@@H]1CC2)C(=O)\C(=C/O)C[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@](C)(O)[C@@]2(C)CC1 ICMWWNHDUZJFDW-DHODBPELSA-N 0.000 description 1
229960005244 oxymetholone Drugs 0.000 description 1
ICMWWNHDUZJFDW-UHFFFAOYSA-N oxymetholone Natural products C1CC2CC(=O)C(=CO)CC2(C)C2C1C1CCC(C)(O)C1(C)CC2 ICMWWNHDUZJFDW-UHFFFAOYSA-N 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
231100000614 poison Toxicity 0.000 description 1
238000011176 pooling Methods 0.000 description 1
239000011591 potassium Substances 0.000 description 1
229910052700 potassium Inorganic materials 0.000 description 1
229960005205 prednisolone Drugs 0.000 description 1
OIGNJSKKLXVSLS-VWUMJDOOSA-N prednisolone Chemical compound O=C1C=C[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 OIGNJSKKLXVSLS-VWUMJDOOSA-N 0.000 description 1
230000001737 promoting effect Effects 0.000 description 1
238000000746 purification Methods 0.000 description 1
238000011084 recovery Methods 0.000 description 1
230000009467 reduction Effects 0.000 description 1
238000000926 separation method Methods 0.000 description 1
239000012090 serum-supplement Substances 0.000 description 1
239000011734 sodium Substances 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
IFGCUJZIWBUILZ-UHFFFAOYSA-N sodium 2-[[2-[[hydroxy-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyphosphoryl]amino]-4-methylpentanoyl]amino]-3-(1H-indol-3-yl)propanoic acid Chemical compound [Na+].C=1NC2=CC=CC=C2C=1CC(C(O)=O)NC(=O)C(CC(C)C)NP(O)(=O)OC1OC(C)C(O)C(O)C1O IFGCUJZIWBUILZ-UHFFFAOYSA-N 0.000 description 1
239000007787 solid Substances 0.000 description 1
230000001954 sterilising effect Effects 0.000 description 1
238000004659 sterilization and disinfection Methods 0.000 description 1
238000003756 stirring Methods 0.000 description 1
239000000126 substance Substances 0.000 description 1
238000006467 substitution reaction Methods 0.000 description 1
239000000758 substrate Substances 0.000 description 1
150000008163 sugars Chemical class 0.000 description 1
239000000725 suspension Substances 0.000 description 1
238000004114 suspension culture Methods 0.000 description 1
238000012360 testing method Methods 0.000 description 1
210000001550 testis Anatomy 0.000 description 1
229960002663 thioctic acid Drugs 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
230000002588 toxic effect Effects 0.000 description 1
239000003440 toxic substance Substances 0.000 description 1
VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 1
229960005486 vaccine Drugs 0.000 description 1
230000035899 viability Effects 0.000 description 1
235000019155 vitamin A Nutrition 0.000 description 1
239000011719 vitamin A Substances 0.000 description 1
MECHNRXZTMCUDQ-RKHKHRCZSA-N vitamin D2 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)/C=C/[C@H](C)C(C)C)=C\C=C1\C[C@@H](O)CCC1=C MECHNRXZTMCUDQ-RKHKHRCZSA-N 0.000 description 1
235000001892 vitamin D2 Nutrition 0.000 description 1
239000011653 vitamin D2 Substances 0.000 description 1
229940045997 vitamin a Drugs 0.000 description 1
150000003751 zinc Chemical class 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
- C12N2501/392—Sexual steroids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/395—Thyroid hormones

Landscapes

Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Health & Medical Sciences (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Cell Biology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

CH4970/83A 1982-09-15 1983-09-13 Composition-supplement pour un milieu de croissance de cellules, procede de sa preparation, composition-finale et utilisation. CH666048A5 (fr)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US41852282A	1982-09-15	1982-09-15

Publications (1)

Publication Number	Publication Date
CH666048A5 true CH666048A5 (fr)	1988-06-30

Family

ID=23658469

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CH4970/83A CH666048A5 (fr)	1982-09-15	1983-09-13	Composition-supplement pour un milieu de croissance de cellules, procede de sa preparation, composition-finale et utilisation.

Country Status (13)

Country	Link
JP (1)	JPH0728728B2 (da)
AU (1)	AU1907783A (da)
BE (1)	BE897760A (da)
CA (1)	CA1221651A (da)
CH (1)	CH666048A5 (da)
DE (1)	DE3333190A1 (da)
DK (1)	DK417483A (da)
FR (1)	FR2532948B1 (da)
GB (1)	GB2127845B (da)
IL (1)	IL69664A0 (da)
IT (1)	IT1217274B (da)
NL (1)	NL8303166A (da)
SE (1)	SE8304795L (da)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4554251A (en) *	1983-11-07	1985-11-19	The Ohio State University Research Foundation	Process and medium for culturing ant venom gland cells
EP0229485A3 (en) *	1986-01-09	1989-02-22	Spectral Bioanalysis Ltd.	Growth-promoting cell culture medium and process for the cultivation of cells employing the same
US4918002A (en) *	1986-08-21	1990-04-17	President And Fellows Of Harvard College	Keratinocyte megacolonies, and assays for growth factors
DE3871206D1 (de) *	1987-03-24	1992-06-25	Grace W R & Co	Basisnaehrmedium fuer eine zellkultur.
ZA882404B (en) *	1987-05-13	1988-09-29	Spectral Bioanalysis, Limited	Growth-suppressing infusion medium,growth-suppressing cell culture medium and processes for the suppression of transformed cell growth
IL94611A (en) *	1989-06-05	1994-12-29	Organogenesis Inc	Medium for cell cultures containing insulin or growth factor similar to insulin, transferrin or iron ion, triiodothyronine or thyroxine and method of use
AU687386B2 (en) *	1993-04-08	1998-02-26	Human Cell Cultures, Inc.	Cell culturing method and medium
EP0812210A4 (en) *	1995-02-23	1998-08-19	Life Medical Sciences Inc	COMPOSITIONS AND METHODS FOR INCREASING HAIR GROWTH AND MAINTAINING THE HAIR COLOR
US5681561A (en) *	1995-06-07	1997-10-28	Life Medical Sciences, Inc.	Compositions and methods for improving autologous fat grafting
JP3553858B2 (ja) *	1999-08-25	2004-08-11	東洋紡績株式会社	血管網類似構造体を有する細胞培養用モジュール
EP3425041A1 (en)	2005-01-05	2019-01-09	Chr. Hansen A/S	Use of compounds involved in biosynthesis of nucleic acids to increase yield of bacterial cultures
US8288366B2 (en) *	2006-06-20	2012-10-16	Chochinov Ronald H	Formulation for hair growth
CN116179473A (zh) *	2023-02-27	2023-05-30	内蒙古奥普赛生物科技有限公司	一种培养Vero细胞的完全培养基及其制备方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IL43343A (en) *	1972-10-24	1976-03-31	Lilly Co Eli	Production of plasminogen activator
USRE30985E (en) *	1978-01-01	1982-06-29		Serum-free cell culture media
JPS5641230A (en) *	1979-09-12	1981-04-17	Teijin Ltd	Preparation of aromatic polyamide film

1983
- 1983-09-05 IL IL69664A patent/IL69664A0/xx unknown
- 1983-09-07 SE SE8304795A patent/SE8304795L/xx not_active Application Discontinuation
- 1983-09-12 GB GB08324369A patent/GB2127845B/en not_active Expired
- 1983-09-13 CH CH4970/83A patent/CH666048A5/fr not_active IP Right Cessation
- 1983-09-13 AU AU19077/83A patent/AU1907783A/en not_active Abandoned
- 1983-09-13 CA CA000436554A patent/CA1221651A/en not_active Expired
- 1983-09-14 NL NL8303166A patent/NL8303166A/nl not_active Application Discontinuation
- 1983-09-14 DE DE19833333190 patent/DE3333190A1/de active Granted
- 1983-09-14 JP JP58168520A patent/JPH0728728B2/ja not_active Expired - Lifetime
- 1983-09-14 IT IT22885/83A patent/IT1217274B/it active
- 1983-09-14 DK DK417483A patent/DK417483A/da not_active Application Discontinuation
- 1983-09-15 FR FR8314715A patent/FR2532948B1/fr not_active Expired
- 1983-09-15 BE BE1/10866A patent/BE897760A/fr not_active IP Right Cessation

Also Published As

Publication number	Publication date
IT1217274B (it)	1990-03-22
SE8304795L (sv)	1984-03-16
DE3333190C2 (da)	1992-11-12
FR2532948B1 (fr)	1988-04-22
JPH0728728B2 (ja)	1995-04-05
JPS5974982A (ja)	1984-04-27
SE8304795D0 (sv)	1983-09-07
DK417483A (da)	1984-03-16
GB2127845B (en)	1986-04-23
IL69664A0 (en)	1983-12-30
BE897760A (fr)	1984-01-02
IT8322885A0 (it)	1983-09-14
GB8324369D0 (en)	1983-10-12
FR2532948A1 (fr)	1984-03-16
AU1907783A (en)	1984-03-22
CA1221651A (en)	1987-05-12
DE3333190A1 (de)	1984-03-22
NL8303166A (nl)	1984-04-02
DK417483D0 (da)	1983-09-14
GB2127845A (en)	1984-04-18

Legal Events

Date	Code	Title	Description
1993-07-15	PUE	Assignment	Owner name: COLLABORATIVE BIOMEDICAL PRODUCTS INC.
2002-05-15	PL	Patent ceased

Publication	Publication Date	Title
CH666048A5 (fr)	1988-06-30	Composition-supplement pour un milieu de croissance de cellules, procede de sa preparation, composition-finale et utilisation.
US5681748A (en)	1997-10-28	Media concentrate technology
JP6367293B2 (ja)	2018-08-01	小ペプチドを含む細胞培養培地
EP0950090B1 (en)	2006-10-11	Serum-free cell culture media
WO1984003710A1 (fr)	1984-09-27	Milieu de culture de cellules animales sans serum et procedes de culture primaire et d'obtention de lignees cellulaires utilisant ce milieu
EP3372671B1 (en)	2021-11-03	Culture medium comprising olgopeptides
WO2005028626A2 (en)	2005-03-31	Cell culture media
WO2018189436A1 (fr)	2018-10-18	Adjuvants dépourvus de cytokines pour milieux de culture cellulaire notamment pour fécondation in vitro, ou pour la culture de follicules, cellules germinales males ou embryons
AU2018231369B2 (en)	2024-08-08	Culture media comprising N-Acyl-X-glutamine dipeptides
Washizu et al.	2000	Optimization of rat hepatocyte culture in citrated human plasma
WO2001046220A2 (en)	2001-06-28	Bioactive oligopeptides
WO2003055990A2 (en)	2003-07-10	Method for culturing and expansion of mammalian undifferentiated epidermal keratinocytes exhibiting stem cell characteristics
CN111492050A (zh)	2020-08-04	用于制备液体培养基的精简型方法
FR2846004A1 (fr)	2004-04-23	Composition pour culture de cellules notamment animales ou de tissus, comprenant du polyethylene glycol
WO2020099225A1 (en)	2020-05-22	Culture medium comprising keto acids
JP2024531754A (ja)	2024-08-29	ジペプチドおよび微量元素を含む組成物
WO2025219232A1 (en)	2025-10-23	Application of small peptides supplemented cell culture media for improved cell performance
Mizrahi et al.	2012	Department of Biotechnology, Israel Institute for Biological Research