CL2018002548A1 - Regulación del metabolismo de ornitina para aumentar el contenido de glicoformas ricas en manosa de proteínas recombinantes.(divisional solicitud 201601786) - Google Patents

Regulación del metabolismo de ornitina para aumentar el contenido de glicoformas ricas en manosa de proteínas recombinantes.(divisional solicitud 201601786)

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Publication number
CL2018002548A1
CL2018002548A1 CL2018002548A CL2018002548A CL2018002548A1 CL 2018002548 A1 CL2018002548 A1 CL 2018002548A1 CL 2018002548 A CL2018002548 A CL 2018002548A CL 2018002548 A CL2018002548 A CL 2018002548A CL 2018002548 A1 CL2018002548 A1 CL 2018002548A1
Authority
CL
Chile
Prior art keywords
cell
inhibitor
ornitine
rich
content
Prior art date
Application number
CL2018002548A
Other languages
English (en)
Inventor
Sohye Kang
Chung-Jr Huang
Hedieh Barkhordarian
Pavel Bondarenko
Zhongqi Zhang
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52273554&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CL2018002548(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of CL2018002548A1 publication Critical patent/CL2018002548A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C12N2500/33—Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/73—Hydrolases (EC 3.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2521/00—Culture process characterised by the use of hydrostatic pressure, flow or shear forces

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Reproductive Health (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

LA INVENCIÓN DIVULGA UN MÉTODO PARA AUMENTAR EL CONTENIDO DE GLICOFORMAS RICAS EN MANOSA DE UNA PROTEÍNA RECOMBINANTE QUE COMPRENDE CULTIVAR UNA CÉLULA HOSPEDERA QUE EXPRESA LA PROTEÍNA RECOMBINANTE EN UN CULTIVO CELULAR QUE COMPRENDE UN COMPUESTO SELECCIONADO DEL GRUPO ORNITINA, ARGININA, UN INHIBIDOR DE ORNITINA AMINOTRANSFERASA, UN INHIBIDOR DE ÓXIDO NÍTRICO SINTASA, Y UN INHIBIDOR DE ARQININA DESCARBOXILASA, DONDE LA PRODUCCIÓN DE ORNITINA EN LA CÉLULA HOSPEDERA DISMINUYE AL AUMENTAR LA ACUMULACIÓN DE ORNITINA CUANDO ES COMPARARLO CON LA CÉLULA HOSPEDERA QUE EXPRESA LA PROTEÍNA RECOMBINANTE CULTIVADA EN UN CULTIVO CELULAR QUE CARECE DE UNO DE LOS COMPUESTOS SELECCIONADOS DEL GRUPO QUE CONSISTE EN ORNITINA, ARGININA, UN INHIBIDOR DE ORNITINA AMINOTRANSFERASA, UN INHIBIDOR DE ÓXIDO NÍTRICO SINTASA Y UN INHIBIDOR DE LA ARGININA DECARBOXILASA. Y LA PROTEÍNA RECOMBINANTE TIENE UN AUMENTO EN EL CONTENIDO DE GLICOFORMAS RICAS EN MANOSA QUE LA PROTEÍNA RECOMBINANTE EXPRESADA EN EL CULTIVO CELULAR QUE CARECE DE UNO DE LOS COMPUESTOS SELECCIONADOS DEL GRUPO QUE CONSISTE EN ORNÍTINA, ARGININA, UN INHIBIDOR DE ORNITINA AMINOTRANSFERASA, UN INHIBIDOR DE ÓXIDO NÍTRICO SINTASA Y UN INHIBIDOR DE LA ARGININA DECARBOXÍLASA.
CL2018002548A 2014-01-13 2018-09-06 Regulación del metabolismo de ornitina para aumentar el contenido de glicoformas ricas en manosa de proteínas recombinantes.(divisional solicitud 201601786) CL2018002548A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201461926481P 2014-01-13 2014-01-13

Publications (1)

Publication Number Publication Date
CL2018002548A1 true CL2018002548A1 (es) 2018-10-26

Family

ID=52273554

Family Applications (3)

Application Number Title Priority Date Filing Date
CL2016001786A CL2016001786A1 (es) 2014-01-13 2016-07-12 Regulación del metabolismo de ornitina para manipular el contenido de glicoformas ricas en manosa de proteínas recombinantes.
CL2018002548A CL2018002548A1 (es) 2014-01-13 2018-09-06 Regulación del metabolismo de ornitina para aumentar el contenido de glicoformas ricas en manosa de proteínas recombinantes.(divisional solicitud 201601786)
CL2022000470A CL2022000470A1 (es) 2014-01-13 2022-02-25 Método para disminuir el contenido de glicoformas ricas en manosa de una proteína recombinante (divisional de la solicitud de patente no. 201601786)

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CL2016001786A CL2016001786A1 (es) 2014-01-13 2016-07-12 Regulación del metabolismo de ornitina para manipular el contenido de glicoformas ricas en manosa de proteínas recombinantes.

Family Applications After (1)

Application Number Title Priority Date Filing Date
CL2022000470A CL2022000470A1 (es) 2014-01-13 2022-02-25 Método para disminuir el contenido de glicoformas ricas en manosa de una proteína recombinante (divisional de la solicitud de patente no. 201601786)

Country Status (14)

Country Link
US (2) US10513723B2 (es)
EP (1) EP3094735A1 (es)
JP (1) JP6732660B2 (es)
KR (1) KR102381791B1 (es)
CN (2) CN112481337B (es)
AU (3) AU2014376225C1 (es)
BR (2) BR112016015797B1 (es)
CA (1) CA2936104C (es)
CL (3) CL2016001786A1 (es)
EA (2) EA039141B1 (es)
IL (1) IL246727B (es)
MX (2) MX376230B (es)
SG (2) SG11201605640UA (es)
WO (1) WO2015105609A1 (es)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR095196A1 (es) 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
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BR112020000127A2 (pt) * 2017-07-06 2020-07-07 Regeneron Pharmaceuticals, Inc. método para selecionar um hidrolisado de soja, e, glicoproteína
RU2672318C1 (ru) * 2017-09-19 2018-11-13 Общество с ограниченной ответственностью "Международный Биотехнологический Центр "Генериум" Способ получения моноклональных антител терапевтического назначения с помощью непрерывного культивирования клеток сно
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Also Published As

Publication number Publication date
US20200071738A1 (en) 2020-03-05
CN106029896A (zh) 2016-10-12
AU2020260443A1 (en) 2020-11-26
EP3094735A1 (en) 2016-11-23
BR122020004385B1 (pt) 2022-10-04
WO2015105609A1 (en) 2015-07-16
MX384153B (es) 2025-03-14
CN112481337A (zh) 2021-03-12
JP6732660B2 (ja) 2020-07-29
AU2014376225B2 (en) 2018-06-07
AU2018226416B2 (en) 2020-08-27
AU2018226416A1 (en) 2018-09-27
KR20160104073A (ko) 2016-09-02
CA2936104C (en) 2022-04-19
AU2014376225A1 (en) 2016-07-21
SG10201806643WA (en) 2018-09-27
AU2020260443B2 (en) 2022-10-27
BR112016015797B1 (pt) 2022-10-11
AU2014376225C1 (en) 2025-06-05
IL246727B (en) 2020-05-31
AU2018226416C1 (en) 2025-11-06
CL2022000470A1 (es) 2022-10-21
EA039141B1 (ru) 2021-12-09
CN106029896B (zh) 2020-10-27
MX376230B (es) 2025-03-07
SG11201605640UA (en) 2016-08-30
CL2016001786A1 (es) 2017-06-02
BR112016015797A2 (es) 2017-10-03
CA2936104A1 (en) 2015-07-16
KR102381791B1 (ko) 2022-03-31
US11254963B2 (en) 2022-02-22
MX2016009084A (es) 2016-10-13
IL246727A0 (en) 2016-08-31
US20160333385A1 (en) 2016-11-17
EA201691426A1 (ru) 2016-11-30
JP2017501749A (ja) 2017-01-19
US10513723B2 (en) 2019-12-24
EA202191909A1 (ru) 2022-01-31
CN112481337B (zh) 2024-06-25

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