CN100587066C - 蛋白质及葡萄糖脱氢酶的精制方法 - Google Patents

蛋白质及葡萄糖脱氢酶的精制方法 Download PDF

Info

Publication number
CN100587066C
CN100587066C CN03824734A CN03824734A CN100587066C CN 100587066 C CN100587066 C CN 100587066C CN 03824734 A CN03824734 A CN 03824734A CN 03824734 A CN03824734 A CN 03824734A CN 100587066 C CN100587066 C CN 100587066C
Authority
CN
China
Prior art keywords
phosphate dehydrogenase
hexose phosphate
purification
elutriant
belongs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CN03824734A
Other languages
English (en)
Chinese (zh)
Other versions
CN1751119A (zh
Inventor
山冈秀亮
黑坂啓介
川瀬至道
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arkray Inc
Original Assignee
Arkray Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arkray Inc filed Critical Arkray Inc
Publication of CN1751119A publication Critical patent/CN1751119A/zh
Application granted granted Critical
Publication of CN100587066C publication Critical patent/CN100587066C/zh
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN03824734A 2002-08-30 2003-08-20 蛋白质及葡萄糖脱氢酶的精制方法 Expired - Lifetime CN100587066C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP253742/2002 2002-08-30
JP2002253742 2002-08-30

Publications (2)

Publication Number Publication Date
CN1751119A CN1751119A (zh) 2006-03-22
CN100587066C true CN100587066C (zh) 2010-02-03

Family

ID=31972809

Family Applications (1)

Application Number Title Priority Date Filing Date
CN03824734A Expired - Lifetime CN100587066C (zh) 2002-08-30 2003-08-20 蛋白质及葡萄糖脱氢酶的精制方法

Country Status (7)

Country Link
US (2) US20060094098A1 (de)
EP (1) EP1535997B1 (de)
JP (1) JP4537850B2 (de)
CN (1) CN100587066C (de)
AT (1) ATE528393T1 (de)
AU (1) AU2003262258A1 (de)
WO (1) WO2004022732A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1304838C (zh) 2001-09-14 2007-03-14 爱科来株式会社 浓度测量方法、浓度测量用具以及浓度测量装置
WO2005023111A1 (ja) * 2003-09-02 2005-03-17 Koji Sode グルコースセンサおよびグルコース濃度測定装置
WO2005103248A1 (ja) 2004-04-23 2005-11-03 Arkray, Inc. 変異グルコース脱水素酵素
JP5598810B2 (ja) * 2009-04-10 2014-10-01 国立大学法人三重大学 コイ由来抗菌剤の製造方法及び抗菌処理装置
TWI400128B (zh) * 2009-11-30 2013-07-01 Univ Tamkang 蛋白質純化方法
WO2012051147A1 (en) * 2010-10-11 2012-04-19 Abbott Laboratories Processes for purification of proteins
CA2821711C (en) 2010-12-15 2017-10-10 Baxter International Inc. Eluate collection using conductivity gradient

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5768138A (en) * 1980-10-11 1982-04-26 Oriental Yeast Co Ltd Chromatography adsorbent and refining method of enzyme using this
DE3711881A1 (de) 1987-04-08 1988-10-27 Merck Patent Gmbh Verfahren zur herstellung von glucosedehydrogenase aus bacillus megaterium
US5250415A (en) * 1987-04-08 1993-10-05 Merck Patent Gesellschaft Mit Beschrankter Haftung Process for the preparation of glucose dehydrogenase from Bacillus megaterium
US5939536A (en) * 1990-03-05 1999-08-17 Genzyme Corporation Methods for purifying cystic fibrosis transmembrane conductance regulation
JP4107386B2 (ja) 2000-10-31 2008-06-25 広司 早出 新規グルコース脱水素酵素及び該脱水素酵素の製造方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Purification of the iron-sulfur protein,ubiquinone-bindingprotein,and cytochrome C1 from a single source ofmitochondrial complex III.Yoshiharu Shimomura et al.Analytical Biochemistry,Vol.153. 1986 *

Also Published As

Publication number Publication date
US9206398B2 (en) 2015-12-08
US20120309072A1 (en) 2012-12-06
WO2004022732A1 (ja) 2004-03-18
JPWO2004022732A1 (ja) 2005-12-22
EP1535997A4 (de) 2007-07-11
EP1535997A1 (de) 2005-06-01
EP1535997B1 (de) 2011-10-12
AU2003262258A1 (en) 2004-03-29
US20060094098A1 (en) 2006-05-04
JP4537850B2 (ja) 2010-09-08
CN1751119A (zh) 2006-03-22
AU2003262258A8 (en) 2004-03-29
ATE528393T1 (de) 2011-10-15

Similar Documents

Publication Publication Date Title
Bóka et al. Putrescine biosensor based on putrescine oxidase from Kocuria rosea
Saito et al. Purification of a blue-green algal deoxyribonucleic acid photoreactivating enzyme. An enzyme requiring light as a physical cofactor to perform its catalytic function
JPS5837833B2 (ja) 微生物リポプロテインリパ−ゼの精製方法
Frings et al. Enzymes involved in anaerobic polyethylene glycol degradation by Pelobacter venetianus and Bacteroides strain PG1
JPH0516833B2 (de)
CN100587066C (zh) 蛋白质及葡萄糖脱氢酶的精制方法
CN104726478A (zh) 表达精氨酸脱亚胺酶基因的重组大肠杆菌及其应用
Tal et al. Purification and characterization of d-β-hydroxybutyrate dehydrogenase from Azospirillum brasilense Cd
US4226764A (en) α-Amylase inhibitor from a streptomycete and process for its preparation
Yazbik et al. Fast and efficient purification of chloroperoxidase from C. fumago
CA1318631C (en) Process for production of superoxide dismutase
US11441129B2 (en) Modified gene sequences encoding choline oxidase and a method for preparing betaine using the same
JP2721536B2 (ja) Ｄ―β―ヒドロキシアミノ酸を取得する方法
JPH0998779A (ja) トレハロース合成酵素、その製法及びそれを用いたトレハロースの製造法
CN117467715A (zh) 多步酶法同时制备d-谷氨酸及l-谷胱甘肽的工艺
JPH0319695A (ja) トランス―４―シアノシクロヘキサンカルボン酸アミドの製造法およびそれに用いる酵素
WO1985004408A1 (fr) Antibiotique tan-588, son procede de preparation et nouvelle souche appartenant au genre empedobacter
JPH047677B2 (de)
JP2690779B2 (ja) Ｌ―アスコルビン酸誘導体及びその製造法
JP2588707B2 (ja) サルコシン・オキシダーゼの製造法
JPS62278982A (ja) Ｎ−アセチルノイラミン酸リア−ゼの製造法
JPS6312279A (ja) 新規アルデヒドデヒドロゲナ−ゼ複合体およびその製造法
JPH01132379A (ja) 新規ウレアーゼ及びその製造方法
Kit et al. Patents and literature
JPS62118882A (ja) Ｌ−アルギニンオキシダ−ゼ及びその製造法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CX01 Expiry of patent term
CX01 Expiry of patent term

Granted publication date: 20100203