CN101410523B - 表达系统 - Google Patents

表达系统 Download PDF

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Publication number
CN101410523B
CN101410523B CN2007800112768A CN200780011276A CN101410523B CN 101410523 B CN101410523 B CN 101410523B CN 2007800112768 A CN2007800112768 A CN 2007800112768A CN 200780011276 A CN200780011276 A CN 200780011276A CN 101410523 B CN101410523 B CN 101410523B
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Prior art keywords
plasmid
promotor
operator gene
gene sequence
sequence
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Chinese (zh)
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CN101410523A (zh
Inventor
I·J·霍奇森
C·D·J·伦农
B·V·卡拉
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Fujifilm Diosynth Biotechnologies UK Ltd
Original Assignee
Fujifilm Diosynth Biotechnologies UK Ltd
Avecia Biologics Ltd
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Priority to CN201210056815.2A priority Critical patent/CN102690834B/zh
Priority to CN201810774783.7A priority patent/CN108841848B/zh
Publication of CN101410523A publication Critical patent/CN101410523A/zh
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Publication of CN101410523B publication Critical patent/CN101410523B/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN2007800112768A 2006-02-03 2007-02-01 表达系统 Active CN101410523B (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201210056815.2A CN102690834B (zh) 2006-02-03 2007-02-01 表达系统
CN201810774783.7A CN108841848B (zh) 2006-02-03 2007-02-01 表达系统

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0602173.7A GB0602173D0 (en) 2006-02-03 2006-02-03 Expression system
GB0602173.7 2006-02-03
PCT/GB2007/000351 WO2007088371A2 (en) 2006-02-03 2007-02-01 Expression system

Related Child Applications (2)

Application Number Title Priority Date Filing Date
CN201810774783.7A Division CN108841848B (zh) 2006-02-03 2007-02-01 表达系统
CN201210056815.2A Division CN102690834B (zh) 2006-02-03 2007-02-01 表达系统

Publications (2)

Publication Number Publication Date
CN101410523A CN101410523A (zh) 2009-04-15
CN101410523B true CN101410523B (zh) 2012-05-16

Family

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Family Applications (3)

Application Number Title Priority Date Filing Date
CN2007800112768A Active CN101410523B (zh) 2006-02-03 2007-02-01 表达系统
CN201810774783.7A Active CN108841848B (zh) 2006-02-03 2007-02-01 表达系统
CN201210056815.2A Active CN102690834B (zh) 2006-02-03 2007-02-01 表达系统

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CN201810774783.7A Active CN108841848B (zh) 2006-02-03 2007-02-01 表达系统
CN201210056815.2A Active CN102690834B (zh) 2006-02-03 2007-02-01 表达系统

Country Status (15)

Country Link
US (4) US8530188B2 (de)
EP (6) EP2390334B1 (de)
JP (5) JP5221385B2 (de)
KR (3) KR101492844B1 (de)
CN (3) CN101410523B (de)
CA (2) CA2959915C (de)
DK (4) DK2386644T3 (de)
ES (3) ES2569075T3 (de)
GB (1) GB0602173D0 (de)
HU (1) HUE027350T2 (de)
PL (3) PL1984506T3 (de)
PT (2) PT2386642E (de)
SG (3) SG169388A1 (de)
WO (1) WO2007088371A2 (de)
ZA (2) ZA200806238B (de)

Families Citing this family (19)

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GB0602173D0 (en) * 2006-02-03 2006-03-15 Avecia Ltd Expression system
GB0709061D0 (en) 2007-05-11 2007-06-20 Avecia Biolog Ltd Expression system
GB0810154D0 (en) * 2008-06-04 2008-07-09 Avecia Biolog Ltd Expression vector
GB0905331D0 (en) * 2009-03-27 2009-05-13 Avecia Biolog Ltd Expression process
GB201018664D0 (en) 2010-11-05 2010-12-22 Msd Biolog Uk Ltd Expression process
GB201021795D0 (en) * 2010-12-21 2011-02-02 Msd Biolog Uk Ltd Expression Process
GB201209896D0 (en) 2012-06-01 2012-07-18 Fujifilm Diosynth Biotechnologies Uk Ltd Process
BR112015017994A2 (pt) 2013-01-31 2017-07-11 Glaxo Group Ltd método para produzir uma proteína recombinante, método para clarificar uma coleta microbiana, e, coleta modificada de célula de escherichia coli
JP6545153B2 (ja) * 2013-05-03 2019-07-17 フジフィルム・ダイオシンス・バイオテクノロジーズ ・ユーケイ・リミテッド 発現方法
GB201311837D0 (en) 2013-07-01 2013-08-14 Fujifilm Diosynth Biotechnologies Uk Ltd Expression Process
GB201319930D0 (en) 2013-11-12 2013-12-25 Fujifilm Diosynth Biotechnologies Uk Ltd Expression process
EP3186379B1 (de) * 2014-08-28 2020-04-08 Synthetic Biologics, Inc. Auf e. coli basierende herstellung von beta-lactamase
US9791697B2 (en) 2015-12-11 2017-10-17 Panasonic Intellectual Property Management Co., Ltd. Screen
SG11201808562SA (en) * 2016-03-29 2018-10-30 Geltor Inc Expression of proteins in gram-negative bacteria wherein the ratio of periplasmic volume to cytoplasmic volume is between 0.5:1 and 10:1
WO2017191255A1 (en) 2016-05-06 2017-11-09 Glaxosmithkline Intellectual Property Development Limited Method of producing a recombinant protein
GB201704659D0 (en) * 2017-03-24 2017-05-10 Fujifilm Diosynth Biotechnologies Uk Ltd Expression system
EP4132958A4 (de) * 2020-04-09 2024-05-15 United States Government as Represented by The Department of Veterans Affairs Zusammensetzungen mit rekombinantem epo und verfahren zur verwendung davon
CN115698276A (zh) * 2020-04-15 2023-02-03 阿拉斯加大学安克雷奇分校 用于生产异丁烯的方法和组合物
CA3238296A1 (en) 2021-12-17 2023-06-22 Daniel Mulvihill Sequences and methods for production of recombinant biological molecules in vesicles

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WO2005071089A2 (en) * 2004-01-12 2005-08-04 Vertex Pharmaceuticals Incorporated Dual host expression vector system and screening methods

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US20170240943A1 (en) 2017-08-24
DK2386642T3 (en) 2014-02-17
EP2695943A1 (de) 2014-02-12
EP2386644A3 (de) 2012-11-28
EP2386642A3 (de) 2012-06-13
EP2386643A3 (de) 2012-11-28
DK2695943T3 (en) 2016-04-18
ES2445173T3 (es) 2014-02-28
WO2007088371A2 (en) 2007-08-09
EP2386644B1 (de) 2015-12-16
KR20140090699A (ko) 2014-07-17
JP2013078323A (ja) 2013-05-02
PL2695943T3 (pl) 2016-07-29
PL2386642T3 (pl) 2014-04-30
PT2386642E (pt) 2014-02-12
EP2390334A3 (de) 2012-11-28
CA2637818A1 (en) 2007-08-09
US20090170160A1 (en) 2009-07-02
EP2386642A2 (de) 2011-11-16
ES2569075T3 (es) 2016-05-06
US8530188B2 (en) 2013-09-10
HUE027350T2 (hu) 2016-09-28
EP2390334A2 (de) 2011-11-30
JP5713984B2 (ja) 2015-05-07
EP2390334B1 (de) 2016-08-10
US20140065670A1 (en) 2014-03-06
JP6571707B2 (ja) 2019-09-04
JP2009525038A (ja) 2009-07-09
JP2016136959A (ja) 2016-08-04
ZA201004904B (en) 2011-03-30
ZA200806238B (en) 2010-10-27
EP2695943B1 (de) 2016-01-20
CN108841848A (zh) 2018-11-20
EP2386643A2 (de) 2011-11-16
GB0602173D0 (en) 2006-03-15
WO2007088371A3 (en) 2007-10-04
EP1984506A2 (de) 2008-10-29
PL1984506T3 (pl) 2013-11-29
EP2386644A2 (de) 2011-11-16
DK1984506T3 (da) 2013-09-16
PT1984506E (pt) 2013-09-06
CN102690834B (zh) 2018-09-04
US11098335B2 (en) 2021-08-24
EP2386642B1 (de) 2013-11-13
JP5221385B2 (ja) 2013-06-26
US20210348205A1 (en) 2021-11-11
KR20140119202A (ko) 2014-10-08
CA2959915A1 (en) 2007-08-09
CN102690834A (zh) 2012-09-26
CN108841848B (zh) 2022-03-29
JP6298481B2 (ja) 2018-03-20
EP2386643B1 (de) 2017-04-12
US9677103B2 (en) 2017-06-13
DK2386644T3 (en) 2016-03-14
EP1984506B1 (de) 2013-06-05
JP2015006193A (ja) 2015-01-15
SG169388A1 (en) 2011-03-30
CA2637818C (en) 2017-05-02
KR101624345B1 (ko) 2016-06-07
CN101410523A (zh) 2009-04-15
SG169389A1 (en) 2011-03-30
KR101512776B1 (ko) 2015-04-16
KR101492844B1 (ko) 2015-02-16
KR20080098013A (ko) 2008-11-06
SG169390A1 (en) 2011-03-30
CA2959915C (en) 2022-11-15
JP2017136072A (ja) 2017-08-10
ES2436883T3 (es) 2014-01-07

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