CN113355357B - 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化 - Google Patents

对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化 Download PDF

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CN113355357B
CN113355357B CN202110502128.8A CN202110502128A CN113355357B CN 113355357 B CN113355357 B CN 113355357B CN 202110502128 A CN202110502128 A CN 202110502128A CN 113355357 B CN113355357 B CN 113355357B
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sequence
cas9
crispr
cas9 protein
protein
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CN113355357A (zh
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F·张
F·蓝
O·沙勒姆
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Massachusetts Institute of Technology
Broad Institute Inc
Harvard University
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Massachusetts Institute of Technology
Broad Institute Inc
Harvard University
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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  • Ophthalmology & Optometry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Epidemiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
CN202110502128.8A 2012-12-12 2013-12-12 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化 Active CN113355357B (zh)

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US201261736527P 2012-12-12 2012-12-12
US61/736,527 2012-12-12
US201361748427P 2013-01-02 2013-01-02
US61/748,427 2013-01-02
US201361758468P 2013-01-30 2013-01-30
US61/758,468 2013-01-30
US201361769046P 2013-02-25 2013-02-25
US61/769,046 2013-02-25
US201361791409P 2013-03-15 2013-03-15
US201361802174P 2013-03-15 2013-03-15
US61/791,409 2013-03-15
US61/802,174 2013-03-15
US201361806375P 2013-03-28 2013-03-28
US61/806,375 2013-03-28
US201361814263P 2013-04-20 2013-04-20
US61/814,263 2013-04-20
US201361819803P 2013-05-06 2013-05-06
US61/819,803 2013-05-06
US201361828130P 2013-05-28 2013-05-28
US61/828,130 2013-05-28
US201361836101P 2013-06-17 2013-06-17
US201361835931P 2013-06-17 2013-06-17
US61/835,931 2013-06-17
US61/836,101 2013-06-17
CN201380072821.XA CN105209621B (zh) 2012-12-12 2013-12-12 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化
PCT/US2013/074691 WO2014093635A1 (en) 2012-12-12 2013-12-12 Engineering and optimization of improved systems, methods and enzyme compositions for sequence manipulation
CN202110502128.8A CN113355357B (zh) 2012-12-12 2013-12-12 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化

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CN201380072821.XA Active CN105209621B (zh) 2012-12-12 2013-12-12 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化

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US (6) US20140186919A1 (2)
EP (1) EP2898075B1 (2)
JP (6) JP6552965B2 (2)
KR (1) KR20150105634A (2)
CN (2) CN113355357B (2)
AU (4) AU2013359212B2 (2)
CA (1) CA2894684A1 (2)
DK (2) DK2898075T3 (2)
ES (2) ES2576126T3 (2)
IL (2) IL300461A (2)
PL (1) PL2898075T3 (2)
PT (1) PT2898075E (2)
SG (3) SG11201504519TA (2)
WO (1) WO2014093635A1 (2)
ZA (1) ZA201504081B (2)

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