CN114225090A - Chitosan-based nanofiber wound dressing with adhesion and antibacterial performance and preparation method thereof - Google Patents
Chitosan-based nanofiber wound dressing with adhesion and antibacterial performance and preparation method thereof Download PDFInfo
- Publication number
- CN114225090A CN114225090A CN202111650654.5A CN202111650654A CN114225090A CN 114225090 A CN114225090 A CN 114225090A CN 202111650654 A CN202111650654 A CN 202111650654A CN 114225090 A CN114225090 A CN 114225090A
- Authority
- CN
- China
- Prior art keywords
- chitosan
- nanofiber
- solution
- wound dressing
- aminolevulinic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/20—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing organic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/24—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4382—Stretched reticular film fibres; Composite fibres; Mixed fibres; Ultrafine fibres; Fibres for artificial leather
- D04H1/43825—Composite fibres
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/236—Glycosaminoglycans, e.g. heparin, hyaluronic acid, chondroitin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/41—Anti-inflammatory agents, e.g. NSAIDs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Materials Engineering (AREA)
- Epidemiology (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Textile Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention provides a chitosan-based nanofiber wound dressing with adhesion and antibacterial performance and a preparation method thereof, wherein the chitosan-based nanofiber wound dressing is core-shell type nanofibers, and a nanofiber membrane with the fiber diameter of 100nm-20 mu m is obtained by electrospinning in a coaxial electrostatic spinning mode. Wherein the polyvinyl alcohol forms the shell of the nanofiber and the chitosan, dopamine hydrochloride and 5-aminolevulinic acid form the core of the nanofiber. Experiments prove that the chitosan-based nanofiber membrane modified by dopamine hydrochloride and 5-aminolevulinic acid has good hemostatic performance and biocompatibility. By adding dopamine hydrochloride and 5-aminolevulinic acid into the chitosan solution, the good water absorption and fibroblasticity of polyvinyl alcohol in the original material and the hemostatic property of chitosan are maintained, and the adhesive property and antibacterial property to biological tissues such as skin, organs and the like are improved, and the wound healing is promoted.
Description
Technical Field
The invention relates to a chitosan-based nanofiber wound dressing with adhesion and antibacterial performance and a preparation method thereof, belonging to the field of biomedical materials.
Background
Traumatic hemorrhage is one of the most common injuries in battlefields and various accident sites, and uncontrolled hemorrhage is the leading cause of death of wounded personnel in the scene. In fact, the casualties resulting from bleeding are mostly avoidable. The bandage widely used at present is combat gauze, but bacterial infection of wounds is also a great factor causing death of wounded persons. Meanwhile, the diabetic patients have serious complications, the wound surface is closed very slowly, the infection rate is high, amputation or limb breakage is caused, the medical cost is high, and the life quality of the patients is poor. In order to improve the survival rate of patients, the wound dressing needs to be upgraded and improved, and the chitosan-based wound dressing is researched more.
Chitosan is a natural high molecular polymer and belongs to aminopolysaccharide. Is the only cationically charged basic polysaccharide found to date. The chitosan has the characteristics of no irritation, no toxicity, antifungal property, good biocompatibility, good oxygen permeability and the like, and can be used for treating various wound surfaces. However, chitosan has poor hydrophilicity, solubility and tissue adhesion, and its antibacterial property against drug-resistant bacteria chitosan is not satisfactory.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the chitosan-based nanofiber wound dressing with adhesion and antibacterial performance and the preparation method thereof. By adopting the method, the excellent hemostatic property of the chitosan is utilized, and the hydrophilicity, the fiber forming property and the mechanical property of the polyvinyl alcohol reinforced material are added. The catechol group in the dopamine interacts with amino or sulfur groups in tissues to show good adhesion with the tissues, so that the dopamine hydrochloride is added into the chitosan solution to enhance the tissue adhesion of the wound dressing.
The 5-aminolevulinic acid is applied to a wound, can be converted into protoporphyrin PpIX in skin tissues, generates active oxygen through laser irradiation to kill bacteria, and meanwhile, the 5-aminolevulinic acid has a good treatment effect on skin inflammation. Therefore, the 5-aminolevulinic acid is added to enhance the antibacterial property of the wound dressing and accelerate the healing of the wound, the membrane structure obtained by the electrostatic spinning mode has better mechanical strength, high internal porosity, large specific surface area and good form adaptability, can be used for irregular form wound surfaces, and has the advantages of green and pollution-free manufacturing process and low price.
A preparation method of chitosan-based nanofiber wound dressing with adhesion and antibacterial performance comprises the following steps:
(1) dissolving chitosan into an acetic acid solution, completely dissolving to obtain a chitosan solution, then adding dopamine hydrochloride and 5-aminolevulinic acid, and uniformly stirring to obtain a 5-aminolevulinic acid-dopamine hydrochloride-chitosan solution;
(2) preparing a polyvinyl alcohol solution by using deionized water;
(3) building an electrostatic spinning device, taking the 5-aminolevulinic acid-dopamine hydrochloride-chitosan solution obtained in the step (1) as a core solution, taking the polyvinyl alcohol solution obtained in the step (2) as a shell solution, respectively filling the two solutions into corresponding syringes, and performing electrospinning in a coaxial electrospinning mode to obtain a nanofiber membrane;
(4) and (3) drying the obtained electrostatic spinning nanofiber membrane for more than 12 hours in vacuum, and disinfecting.
Further, the volume concentration of the acetic acid in the step (1) is 1% V/V.
Further, the concentration of the chitosan in the chitosan solution in the step (1) is 1-3% M/V.
Further, the mass ratio of the chitosan, the dopamine hydrochloride and the 5-aminolevulinic acid added in the step (1) is 80:6: 15.
Further, the concentration of the polyvinyl alcohol in the step (2) is 10% M/V.
Further, the voltage for carrying out coaxial electrospinning by using an electrostatic spinning device in the step (3) is 10-15 kV; the distance between the needle of the electrostatic spinning device and the collecting device is 10-20 cm.
Further, the collecting device in the step (3) is a rotary drum.
Further, the syringe advancing speed in the step (3) is 0.2-0.35 ml/h.
According to the chitosan-based nanofiber wound dressing prepared by the method, the chitosan-based nanofiber wound dressing is core-shell type nanofibers, and a nanofiber membrane is obtained by electrospinning in a coaxial electrostatic spinning mode, wherein polyvinyl alcohol forms a shell of the nanofibers, and chitosan, dopamine hydrochloride and 5-aminolevulinic acid form a core of the nanofibers.
Further, the diameter of the fiber of the nanofiber membrane is 100nm-20 μm.
Advantageous effects
(1) The nanofiber membrane provided by the invention takes chitosan as a main body, dopamine hydrochloride is added, the dopamine can enhance the tissue adhesion of the material, and both the dopamine and the material have good biocompatibility.
(2) The nanofiber membrane provided by the invention takes chitosan as a main body, and 5-aminolevulinic acid is added to enhance the antibacterial property of the wound dressing and accelerate wound healing.
(3) According to the invention, 5-aminolevulinic acid is added into the electrospinning nuclear solution, and the sustained release can be realized by adopting a coaxial electrospinning mode, so that the effect of controlling the drug release is achieved.
(4) By adding dopamine hydrochloride and 5-aminolevulinic acid into the chitosan solution, the good water absorption and fibroblasticity of polyvinyl alcohol in the original material and the hemostatic property of chitosan are maintained, and the adhesive property and antibacterial property to biological tissues such as skin, organs and the like are improved, and the wound healing is promoted.
Drawings
FIG. 1 is an infrared spectrum of a sample of example 2 of the present invention and a sample of example 3.
FIG. 2 is a SEM image of a sample of example 2 of the present invention.
FIG. 3 is a bar graph of the adhesion strength of the experimental sample of the pigskin adhesion of the sample of example 1, example 2 and example 3 of the present invention.
FIG. 4 is a bar graph of bacterial survival for the samples of example 1, example 2, example 3, and example 3 plus laser according to the present invention.
FIG. 5 is a histogram showing the blood coagulation times of the samples of example 1, example 2 and example 3 of the present invention.
FIG. 6 optical photograph of mouse liver adhesion of example 3 sample of the present invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
The invention adopts the following technical scheme for solving the technical problems:
example 1:
1 g of polyvinyl alcohol was dissolved in 10ml of deionized water to obtain a polyvinyl alcohol solution having a concentration of 10% M/V.
And (3) carrying out electrostatic spinning on the polyvinyl alcohol solution, wherein the electrostatic spinning condition is high pressure of 15kv, the receiving distance is 10cm, the propelling speed of an injector is 0.3ml/h, the rotating speed of a roller collector is 500rpm, after the electrospinning is finished, collecting the obtained sample, carrying out vacuum drying for more than 12h, and storing the sample.
Example 2:
1 g of polyvinyl alcohol was dissolved in 10ml of deionized water to obtain a polyvinyl alcohol solution having a concentration of 10% M/V. 0.3 g of chitosan was dissolved in 1% V/V acetic acid solution by volume to give a 3% M/V chitosan solution.
And (3) taking the polyvinyl alcohol solution as a shell solution and the chitosan solution as a core solution, and injecting the solutions into corresponding injectors. And (3) carrying out coaxial electrostatic spinning under the conditions of high pressure of 15kv, receiving distance of 10cm, injector propulsion speed of 0.3ml/h and rotating speed of 500rpm of a roller collector, collecting the obtained sample after the electrospinning is finished, carrying out vacuum drying for more than 12h, and storing the sample.
Example 3:
1 g of polyvinyl alcohol was dissolved in 10ml of deionized water to obtain a polyvinyl alcohol solution having a concentration of 10% M/V. 0.3 g of chitosan is dissolved in 1% V/V acetic acid solution by volume concentration to obtain 3% M/V chitosan solution, 0.02 g of dopamine hydrochloride and 0.05 g of 5-aminolevulinic acid are dissolved in the chitosan solution to obtain 5-aminolevulinic acid-dopamine hydrochloride-chitosan solution. And (3) taking the polyvinyl alcohol solution as a shell solution and the chitosan-dopamine hydrochloride solution as a core solution, and injecting the solutions into corresponding injectors. And (3) carrying out coaxial electrostatic spinning under the conditions of high pressure of 15kv, receiving distance of 10cm, injector propulsion speed of 0.3ml/h and rotating speed of 500rpm of a roller collector, collecting the obtained sample after the electrospinning is finished, carrying out vacuum drying for more than 12h, and storing the sample.
Claims (10)
1. A preparation method of chitosan-based nanofiber wound dressing with adhesion and antibacterial performance is characterized by comprising the following steps:
(1) dissolving chitosan into an acetic acid solution, completely dissolving to obtain a chitosan solution, then adding dopamine hydrochloride and 5-aminolevulinic acid, and uniformly stirring to obtain a 5-aminolevulinic acid-dopamine hydrochloride-chitosan solution;
(2) preparing a polyvinyl alcohol solution by using deionized water;
(3) building an electrostatic spinning device, taking the 5-aminolevulinic acid-dopamine hydrochloride-chitosan solution obtained in the step (1) as a core solution, taking the polyvinyl alcohol solution obtained in the step (2) as a shell solution, respectively filling the two solutions into corresponding syringes, and performing electrospinning in a coaxial electrospinning mode to obtain a nanofiber membrane;
(4) and (3) drying the obtained electrostatic spinning nanofiber membrane for more than 12 hours in vacuum, and disinfecting.
2. The method according to claim 1, wherein the volume concentration of acetic acid in the step (1) is 1% V/V.
3. The method according to claim 1, wherein the concentration of chitosan in the chitosan solution in the step (1) is 1-3% M/V.
4. The preparation method according to claim 1, wherein the mass ratio of the chitosan, the dopamine hydrochloride and the 5-aminolevulinic acid added in the step (1) is 80:6: 15.
5. The method according to claim 1, wherein the concentration of the polyvinyl alcohol in the step (2) is 10% M/V.
6. The preparation method according to claim 1, wherein the voltage for the coaxial electrospinning in the step (3) by using the electrospinning device is 10-15 kV; the distance between the needle of the electrostatic spinning device and the collecting device is 10-20 cm.
7. The method according to claim 6, wherein the collecting device in the step (3) is a rotating drum.
8. The method according to claim 1, wherein the syringe advance speed in the step (3) is 0.2 to 0.35 ml/h.
9. The chitosan-based nanofiber wound dressing prepared according to any one of claims 1 to 8, wherein the chitosan-based nanofiber wound dressing is a core-shell type nanofiber, and a nanofiber membrane is obtained by electrospinning in a coaxial electrospinning manner, wherein polyvinyl alcohol forms a shell of the nanofiber, and chitosan, dopamine hydrochloride and 5-aminolevulinic acid form a core of the nanofiber.
10. A chitosan-based nanofiber wound dressing with adhesive and antibacterial properties, according to claim 9, characterized in that the nanofiber membrane has a fiber diameter of 100nm-20 μ ι η.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111650654.5A CN114225090A (en) | 2021-12-30 | 2021-12-30 | Chitosan-based nanofiber wound dressing with adhesion and antibacterial performance and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111650654.5A CN114225090A (en) | 2021-12-30 | 2021-12-30 | Chitosan-based nanofiber wound dressing with adhesion and antibacterial performance and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114225090A true CN114225090A (en) | 2022-03-25 |
Family
ID=80744647
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111650654.5A Pending CN114225090A (en) | 2021-12-30 | 2021-12-30 | Chitosan-based nanofiber wound dressing with adhesion and antibacterial performance and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114225090A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114849052A (en) * | 2022-04-24 | 2022-08-05 | 浙江理工大学 | Microneedle patch for repairing diabetic skin wounds and preparation method thereof |
| CN115262223A (en) * | 2022-08-23 | 2022-11-01 | 青岛大学 | A kind of polyester/chitosan gel composite fiber membrane and preparation method thereof |
| CN116077350A (en) * | 2022-09-15 | 2023-05-09 | 山东福瑞达生物股份有限公司 | A kind of electrospun composite nanofiber film, preparation method and application |
| CN116942650A (en) * | 2023-08-04 | 2023-10-27 | 宁波见睿新材料有限公司 | New application of 5-aminolevulinic acid in preparation of procoagulant or hemostatic drugs |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103611182A (en) * | 2013-12-10 | 2014-03-05 | 东华大学 | Preparation method of core-shell structure superfine fiber carrier material for medical dressing |
| CN107469140A (en) * | 2017-08-14 | 2017-12-15 | 高鼎精细化工(昆山)有限公司 | A kind of photodynamics antiseptic dressing for having antibacterial functions, preparation method and application |
-
2021
- 2021-12-30 CN CN202111650654.5A patent/CN114225090A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103611182A (en) * | 2013-12-10 | 2014-03-05 | 东华大学 | Preparation method of core-shell structure superfine fiber carrier material for medical dressing |
| CN107469140A (en) * | 2017-08-14 | 2017-12-15 | 高鼎精细化工(昆山)有限公司 | A kind of photodynamics antiseptic dressing for having antibacterial functions, preparation method and application |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114849052A (en) * | 2022-04-24 | 2022-08-05 | 浙江理工大学 | Microneedle patch for repairing diabetic skin wounds and preparation method thereof |
| CN115262223A (en) * | 2022-08-23 | 2022-11-01 | 青岛大学 | A kind of polyester/chitosan gel composite fiber membrane and preparation method thereof |
| CN115262223B (en) * | 2022-08-23 | 2023-12-26 | 青岛大学 | Polyester/chitosan gel composite fiber film and preparation method thereof |
| CN116077350A (en) * | 2022-09-15 | 2023-05-09 | 山东福瑞达生物股份有限公司 | A kind of electrospun composite nanofiber film, preparation method and application |
| CN116942650A (en) * | 2023-08-04 | 2023-10-27 | 宁波见睿新材料有限公司 | New application of 5-aminolevulinic acid in preparation of procoagulant or hemostatic drugs |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN114225090A (en) | Chitosan-based nanofiber wound dressing with adhesion and antibacterial performance and preparation method thereof | |
| TWI445555B (en) | Dressing material containing active constituents of Centella asiatica and application thereof | |
| CN100467680C (en) | Antibacterial silk composite nanofiber material and preparation method thereof | |
| CN111945301B (en) | Membrane for releasing nitric oxide based on near-infrared response, preparation method and application | |
| CN107349471A (en) | A kind of complex tissue repair materials of carried medicine sustained-release and preparation method thereof | |
| CN107320786B (en) | Slow-release anti-infection composite soft tissue repair material and preparation method thereof | |
| CN103480042B (en) | Artificial dura mater spinalis, and preparation method and use method thereof | |
| CN103480027B (en) | Preparation method of bacterial cellulose composite chitosan fiber moist dressing | |
| CN102552964B (en) | Nano silver chitosan composite antibacterial composition, adhesive bandage and preparation method of adhesive bandage | |
| CN114949325A (en) | A kind of preparation method of composite nanofiber membrane for wound dressing and composite nanofiber membrane | |
| CN103041440A (en) | Preparation method of recombinant spider silk protein/silver nano biological wound membrane | |
| CN115845114B (en) | Nanofiber medical wound dressing and preparation method thereof | |
| CN106620813A (en) | Preparation method of medical antibacterial nano dressing | |
| CN112587717A (en) | Metal cation crosslinked alginate/bacterial cellulose composite hydrogel antibacterial dressing | |
| CN104342775A (en) | Method for preparing composite nano-fiber membrane with honey and natural materials on basis of environment-friendly electrospinning technology | |
| CN111617303A (en) | A kind of preparation method of high-strength nanofiber membrane wound dressing | |
| CN117188045A (en) | A multilayer nanofiber membrane, its preparation method and its application in diabetic wound dressings | |
| CN115487337A (en) | Dressing patch for skin repair and preparation method thereof | |
| CN113005633A (en) | Antibacterial nanofiber membrane and preparation method and application thereof | |
| CN1239207C (en) | Artificial biological canula and its making process | |
| CN117569013A (en) | Micro-nano fiber membrane containing collagen fibers and preparation method and application thereof | |
| CN105727358B (en) | Drug-loaded biological high-molecular composite nanometer tunica fibrosa and preparation method thereof | |
| CN116036342A (en) | Nanofiber dressing and preparation method and application thereof | |
| CN116370692A (en) | A kind of restoration material and preparation method thereof | |
| CN112915252B (en) | Chitosan quaternary ammonium salt derivative wound dressing and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220325 |
|
| RJ01 | Rejection of invention patent application after publication |