CN1216988C - 分泌型Kex衍生物的制造方法 - Google Patents

分泌型Kex衍生物的制造方法 Download PDF

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Publication number
CN1216988C
CN1216988C CN971116792A CN97111679A CN1216988C CN 1216988 C CN1216988 C CN 1216988C CN 971116792 A CN971116792 A CN 971116792A CN 97111679 A CN97111679 A CN 97111679A CN 1216988 C CN1216988 C CN 1216988C
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CN
China
Prior art keywords
kex2
sequence
amino acid
sequence number
yeast
Prior art date
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Expired - Lifetime
Application number
CN971116792A
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English (en)
Chinese (zh)
Other versions
CN1168922A (zh
Inventor
孙田浩二
增田丰文
铃木雄司
薮田雅之
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Asubio Pharma Co Ltd
Original Assignee
Daiichi Suntory Pharma Co Ltd
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Publication date
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Publication of CN1168922A publication Critical patent/CN1168922A/zh
Application granted granted Critical
Publication of CN1216988C publication Critical patent/CN1216988C/zh
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Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Seasonings (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
CN971116792A 1996-03-04 1997-03-04 分泌型Kex衍生物的制造方法 Expired - Lifetime CN1216988C (zh)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
JP73217/96 1996-03-04
JP7321796 1996-03-04
JP73217/1996 1996-03-04
JP35258096 1996-12-16
JP352580/1996 1996-12-16
JP352580/96 1996-12-16

Publications (2)

Publication Number Publication Date
CN1168922A CN1168922A (zh) 1997-12-31
CN1216988C true CN1216988C (zh) 2005-08-31

Family

ID=26414373

Family Applications (1)

Application Number Title Priority Date Filing Date
CN971116792A Expired - Lifetime CN1216988C (zh) 1996-03-04 1997-03-04 分泌型Kex衍生物的制造方法

Country Status (10)

Country Link
US (1) US5885821A (fr)
EP (1) EP0794254B1 (fr)
KR (1) KR100548653B1 (fr)
CN (1) CN1216988C (fr)
AT (1) ATE409748T1 (fr)
AU (1) AU731201B2 (fr)
CA (1) CA2198968C (fr)
DE (1) DE69739013D1 (fr)
ES (1) ES2312171T3 (fr)
TW (1) TW557300B (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2198966C (fr) * 1996-03-04 2011-06-21 Yuji Suzuki Methode de clivage d'une proteine chimere a l'aide d'une enzyme de traitement
US6517974B1 (en) * 1998-01-30 2003-02-11 Canon Kabushiki Kaisha Lithium secondary battery and method of manufacturing the lithium secondary battery
SK782002A3 (en) 1999-07-21 2003-08-05 Lexigen Pharm Corp FC fusion proteins for enhancing the immunogenicity of protein and peptide antigens
CN100491395C (zh) 2002-04-11 2009-05-27 阿斯比奥制药株式会社 修饰肽的制造方法
AU2003255988A1 (en) * 2002-09-13 2004-04-30 Edupuganti B. Raju Yeast protein expression secretion system
PL2298347T3 (pl) 2003-05-06 2016-03-31 Bioverativ Therapeutics Inc Białka chimeryczne czynnika krzepnięcia do leczenia zaburzenia hemostazy
US7348004B2 (en) 2003-05-06 2008-03-25 Syntonix Pharmaceuticals, Inc. Immunoglobulin chimeric monomer-dimer hybrids
TWI353991B (en) * 2003-05-06 2011-12-11 Syntonix Pharmaceuticals Inc Immunoglobulin chimeric monomer-dimer hybrids
AU2004238263A1 (en) * 2003-05-06 2004-11-25 Syntonix Pharmaceuticals, Inc. Inhibition of drug binding to serum albumin
EP2831262B1 (fr) 2012-03-29 2018-02-14 Biocon Limited Sécrétion d'insuline glargine fonctionnelle directement dans le milieu de culture par l'intermédiaire de la surexpression intracellulaire de kex2p
EP2964666B1 (fr) 2013-03-06 2020-11-18 GlaxoSmithKline LLC Cellules hôtes et procédés d'utilisation
WO2014144549A1 (fr) 2013-03-15 2014-09-18 Biogen Idec Ma Inc. Préparations contenant un polypeptide du facteur ix
CN106337042B (zh) * 2015-07-16 2019-08-09 上海雅心生物技术有限公司 双碱基酶kex2变体及其高效表达方法
CN110982808A (zh) * 2019-12-27 2020-04-10 万新医药科技(苏州)有限公司 Kex2酶的变体及稳定表达的方法
CN114717282A (zh) * 2021-12-31 2022-07-08 北京盛拓达生物技术有限公司 一种组合物及其应用
CN118086261B (zh) * 2024-03-27 2024-08-13 铭诚惠众(江苏)药物研究有限公司 一种高稳定性和高酶活的Kex2蛋白酶突变体

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2643968B2 (ja) * 1988-02-03 1997-08-25 サントリー株式会社 Ｋｅｘ２エンドプロテアーゼ及びその製造方法
JP3113435B2 (ja) * 1992-02-28 2000-11-27 サントリー株式会社 メタノールおよび／またはグリセロール添加により誘導可能なプロモーターを有する新規ベクター

Also Published As

Publication number Publication date
KR970065558A (ko) 1997-10-13
TW557300B (en) 2003-10-11
ES2312171T3 (es) 2009-02-16
CA2198968A1 (fr) 1997-09-04
CN1168922A (zh) 1997-12-31
AU1503797A (en) 1997-09-11
EP0794254A3 (fr) 2000-01-05
ATE409748T1 (de) 2008-10-15
EP0794254A2 (fr) 1997-09-10
EP0794254B1 (fr) 2008-10-01
DE69739013D1 (de) 2008-11-13
AU731201B2 (en) 2001-03-29
US5885821A (en) 1999-03-23
KR100548653B1 (ko) 2006-07-14
CA2198968C (fr) 2010-02-09

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Effective date: 20030425

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Owner name: THE FIRST ASHBIAO PHARMACEUTICAL CO., LTD.

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Address after: Osaka Japan

Patentee after: Daiichi Asubio Pharma Co., Ltd.

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Patentee before: Daiichi Suntory Pharma Co., Ltd.

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Owner name: ASTERIX PHARMACEUTICAL CO., LTD.

Free format text: FORMER NAME OR ADDRESS: THE FIRST ASHBIAO PHARMACEUTICAL CO., LTD.

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Patentee after: Asubio Pharma Co., Ltd.

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Patentee before: Daiichi Asubio Pharma Co., Ltd.

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Granted publication date: 20050831