ATE409748T1 - Verfahren zur herstellung sekretorischer kex2- derivate - Google Patents
Verfahren zur herstellung sekretorischer kex2- derivateInfo
- Publication number
- ATE409748T1 ATE409748T1 AT97301429T AT97301429T ATE409748T1 AT E409748 T1 ATE409748 T1 AT E409748T1 AT 97301429 T AT97301429 T AT 97301429T AT 97301429 T AT97301429 T AT 97301429T AT E409748 T1 ATE409748 T1 AT E409748T1
- Authority
- AT
- Austria
- Prior art keywords
- derivatives
- kex2 protease
- acid sequence
- amino acid
- terminus
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 101150045458 KEX2 gene Proteins 0.000 title 1
- 230000003248 secreting effect Effects 0.000 title 1
- 101001007681 Candida albicans (strain WO-1) Kexin Proteins 0.000 abstract 4
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Seasonings (AREA)
- Enzymes And Modification Thereof (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7321796 | 1996-03-04 | ||
| JP35258096 | 1996-12-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE409748T1 true ATE409748T1 (de) | 2008-10-15 |
Family
ID=26414373
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT97301429T ATE409748T1 (de) | 1996-03-04 | 1997-03-04 | Verfahren zur herstellung sekretorischer kex2- derivate |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US5885821A (de) |
| EP (1) | EP0794254B1 (de) |
| KR (1) | KR100548653B1 (de) |
| CN (1) | CN1216988C (de) |
| AT (1) | ATE409748T1 (de) |
| AU (1) | AU731201B2 (de) |
| CA (1) | CA2198968C (de) |
| DE (1) | DE69739013D1 (de) |
| ES (1) | ES2312171T3 (de) |
| TW (1) | TW557300B (de) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2198966C (en) * | 1996-03-04 | 2011-06-21 | Yuji Suzuki | Method for cleaving chimeric protein using processing enzyme |
| US6517974B1 (en) * | 1998-01-30 | 2003-02-11 | Canon Kabushiki Kaisha | Lithium secondary battery and method of manufacturing the lithium secondary battery |
| SK782002A3 (en) | 1999-07-21 | 2003-08-05 | Lexigen Pharm Corp | FC fusion proteins for enhancing the immunogenicity of protein and peptide antigens |
| US7138489B2 (en) | 2002-04-11 | 2006-11-21 | Daiichi Asubio Pharma Co., Ltd. | Method for producing a modified peptide |
| WO2004024862A2 (en) * | 2002-09-13 | 2004-03-25 | Wockhardt Limited | Yeast protein expression secretion system |
| TWI353991B (en) * | 2003-05-06 | 2011-12-11 | Syntonix Pharmaceuticals Inc | Immunoglobulin chimeric monomer-dimer hybrids |
| AU2004238263A1 (en) * | 2003-05-06 | 2004-11-25 | Syntonix Pharmaceuticals, Inc. | Inhibition of drug binding to serum albumin |
| PT1624891E (pt) | 2003-05-06 | 2010-01-05 | Syntonix Pharmaceuticals Inc | Proteínas quiméricas de factor de coagulação-fc destinadas ao tratamento da hemofilia |
| US7348004B2 (en) | 2003-05-06 | 2008-03-25 | Syntonix Pharmaceuticals, Inc. | Immunoglobulin chimeric monomer-dimer hybrids |
| EP2831262B1 (de) | 2012-03-29 | 2018-02-14 | Biocon Limited | Sekretion von funktionellem insulinglargin direkt in das kulturmedium durch intrazelluläre überexpression von kex2p |
| EP2964666B1 (de) | 2013-03-06 | 2020-11-18 | GlaxoSmithKline LLC | Wirtszellen und verfahren zur verwendung |
| EA037906B1 (ru) | 2013-03-15 | 2021-06-04 | Биовератив Терапьютикс Инк. | Препараты полипептида фактора ix |
| CN106337042B (zh) * | 2015-07-16 | 2019-08-09 | 上海雅心生物技术有限公司 | 双碱基酶kex2变体及其高效表达方法 |
| CN110982808A (zh) * | 2019-12-27 | 2020-04-10 | 万新医药科技(苏州)有限公司 | Kex2酶的变体及稳定表达的方法 |
| CN114717282A (zh) * | 2021-12-31 | 2022-07-08 | 北京盛拓达生物技术有限公司 | 一种组合物及其应用 |
| CN118086261B (zh) * | 2024-03-27 | 2024-08-13 | 铭诚惠众(江苏)药物研究有限公司 | 一种高稳定性和高酶活的Kex2蛋白酶突变体 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2643968B2 (ja) * | 1988-02-03 | 1997-08-25 | サントリー株式会社 | Ｋｅｘ２エンドプロテアーゼ及びその製造方法 |
| ES2093294T3 (es) * | 1992-02-28 | 1996-12-16 | Suntory Ltd | Nuevo vector que comprende un promotor inducible por el metanol y/o glicerol. |
-
1997
- 1997-03-03 CA CA2198968A patent/CA2198968C/en not_active Expired - Lifetime
- 1997-03-04 KR KR1019970007017A patent/KR100548653B1/ko not_active Expired - Lifetime
- 1997-03-04 ES ES97301429T patent/ES2312171T3/es not_active Expired - Lifetime
- 1997-03-04 EP EP97301429A patent/EP0794254B1/de not_active Expired - Lifetime
- 1997-03-04 US US08/805,918 patent/US5885821A/en not_active Expired - Lifetime
- 1997-03-04 AU AU15037/97A patent/AU731201B2/en not_active Expired
- 1997-03-04 TW TW086102703A patent/TW557300B/zh not_active IP Right Cessation
- 1997-03-04 DE DE69739013T patent/DE69739013D1/de not_active Expired - Lifetime
- 1997-03-04 AT AT97301429T patent/ATE409748T1/de active
- 1997-03-04 CN CN971116792A patent/CN1216988C/zh not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| AU1503797A (en) | 1997-09-11 |
| AU731201B2 (en) | 2001-03-29 |
| EP0794254A2 (de) | 1997-09-10 |
| KR970065558A (ko) | 1997-10-13 |
| EP0794254A3 (de) | 2000-01-05 |
| TW557300B (en) | 2003-10-11 |
| DE69739013D1 (de) | 2008-11-13 |
| EP0794254B1 (de) | 2008-10-01 |
| ES2312171T3 (es) | 2009-02-16 |
| CA2198968A1 (en) | 1997-09-04 |
| CN1216988C (zh) | 2005-08-31 |
| CA2198968C (en) | 2010-02-09 |
| US5885821A (en) | 1999-03-23 |
| CN1168922A (zh) | 1997-12-31 |
| KR100548653B1 (ko) | 2006-07-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE409748T1 (de) | Verfahren zur herstellung sekretorischer kex2- derivate | |
| ATE415469T1 (de) | Verfahren zur gewinnung von heterologen polypeptiden aus bakterienzellen | |
| DE60021318D1 (de) | Verfahren zur Herstellung von natürlich gefalteten Proteinen durch Co-Sekretion von molekularen Chaperonen | |
| ATE310090T1 (de) | Gereinigte coprinus laccasen und nukleinsäuren welche dafür kodieren | |
| DE60231055D1 (de) | Reinigung von peptiden unter verwendung von metall-ion-affinitätschromatographie | |
| CA2180103A1 (en) | Gene encoding adseverin | |
| DE3586773D1 (de) | Verfahren zur beseitigung von n-endaminosaeureresten aus eukaryotpolypeptidanlagen und so erzeugte polypeptide. | |
| EP1688430A3 (de) | Ausgeschiedene und Transmembranpolypeptide und dafür kodierende Nukleinsäure | |
| AU3055495A (en) | Recombinant mersacidin and a method for production | |
| GB9719496D0 (en) | G protien chimeras | |
| EP0953644A4 (de) | Systeme zur massenherstellung von proteinen oder peptiden durch mikroorganismen der gattung humicola | |
| AU7505796A (en) | Hyperthermostable protease gene | |
| EP0139501A3 (de) | Herstellung und Expression von Genen für Thaumatin | |
| DE59108128D1 (de) | Verfahren zur Herstellung von Fremdproteinen in Streptomyceten | |
| DE60328813D1 (de) | Mutanten für die herstellung von d-aminosäuren | |
| ATE122099T1 (de) | Verfahren zur herstellung von c-terminalen alpha- amidierten peptiden. | |
| ATE154609T1 (de) | Nicht-lineare peptide, die hydropatisch komplementär zu bekannten aminosäuresequenzen sind, verfahren zur herstellung und verwendung davon | |
| DK595187D0 (da) | Minactivin og fremgangsmaade til fremstilling deraf | |
| ATE67243T1 (de) | Verfahren zur herstellung von insulinaehnlichem wachstumsfaktor i (igf-i) und plasmid zu dessen herstellung. | |
| ATE196927T1 (de) | Verfahren zur herstellung von proteaseinhibitoren | |
| DK0828004T3 (da) | Proteindisulfidisomerase-gen afledt af stamme af methylotrof gær | |
| EP1241186A3 (de) | Menschliches gas-6 (growth arrest-specific gene 6) Protein und dafür kodierende Nukleinsäure | |
| EP1300417A3 (de) | Sekretierte und transmembrane Polypeptide und dafür kodierende Nukleinsäuren | |
| EP1241180A3 (de) | Menschliches A33-Antigen-ähnliches Protein und dafür kodierende Nukleinsäure | |
| AU2001277717A1 (en) | Novel proteins and dnas thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 0794254 Country of ref document: EP |