CU20080242A7 - Método para producir lipasa a partir de una célula de yarrowia lipolytica transformada y preparación que la contiene - Google Patents

Método para producir lipasa a partir de una célula de yarrowia lipolytica transformada y preparación que la contiene

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Publication number
CU20080242A7
CU20080242A7 CU20080242A CU20080242A CU20080242A7 CU 20080242 A7 CU20080242 A7 CU 20080242A7 CU 20080242 A CU20080242 A CU 20080242A CU 20080242 A CU20080242 A CU 20080242A CU 20080242 A7 CU20080242 A7 CU 20080242A7
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CU
Cuba
Prior art keywords
cell
preparation containing
producing lipase
transformed yarrowia
producing
Prior art date
Application number
CU20080242A
Other languages
English (en)
Other versions
CU24009B1 (es
Inventor
Jean-Louis Uribelarrea
Alain Marty
Nicolas Mouz
Yves Leblond
Original Assignee
Mayoly Spindler Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mayoly Spindler Lab filed Critical Mayoly Spindler Lab
Publication of CU20080242A7 publication Critical patent/CU20080242A7/es
Publication of CU24009B1 publication Critical patent/CU24009B1/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • C12N9/20Triglyceride splitting, e.g. by means of lipase
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46Hydrolases (3)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/14Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00Drugs for disorders of the metabolism
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/01Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003Triacylglycerol lipase (3.1.1.3)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Diabetes (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Método de producción de una liposa recombinante ácido-resistente de Yarrowia lipolytica utilizando un medio de cultivo sin cualquier producto de origen animal o mezclas no caracterizadas como la triptona, peptona o lactosuero, así como sus aplicaciones. La cepa recombinante de Yarrowia lipolytica que produce una cantidad excesiva de lipasa Lip2, referida como a YL-LIP2-6C y depositada con la Colección Nacional de Cultivos de Microorganismos (C.N.C.M) bajo el número I-3542, el 15 de diciembre de 2005, y sus usos.
CU2008000242A 2006-06-15 2008-12-15 Método de producción de lipasa recombinante lip2 CU24009B1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/FR2006/001352 WO2007144475A1 (fr) 2006-06-15 2006-06-15 Procede de production de lipase, cellule de yarrowia lipolytica transformee apte a produire ladite lipase et leurs applications

Publications (2)

Publication Number Publication Date
CU20080242A7 true CU20080242A7 (es) 2012-06-21
CU24009B1 CU24009B1 (es) 2014-06-27

Family

ID=37591884

Family Applications (1)

Application Number Title Priority Date Filing Date
CU2008000242A CU24009B1 (es) 2006-06-15 2008-12-15 Método de producción de lipasa recombinante lip2

Country Status (22)

Country Link
US (2) US8334130B2 (es)
EP (1) EP2035556B1 (es)
JP (1) JP5592648B2 (es)
KR (1) KR101497159B1 (es)
CN (1) CN101506359B (es)
AU (1) AU2006344466B2 (es)
BR (1) BRPI0621829B8 (es)
CA (1) CA2657949C (es)
CR (1) CR10515A (es)
CU (1) CU24009B1 (es)
DK (1) DK2035556T3 (es)
EA (1) EA017963B1 (es)
EC (1) ECSP099057A (es)
ES (1) ES2550537T3 (es)
MX (1) MX2008015978A (es)
NO (1) NO342291B1 (es)
NZ (1) NZ573601A (es)
PL (1) PL2035556T3 (es)
PT (1) PT2035556E (es)
SI (1) SI2035556T1 (es)
UA (1) UA97246C2 (es)
WO (1) WO2007144475A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2355754C1 (ru) * 2007-12-25 2009-05-20 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП ГосНИИгенетика) ШТАММ ДРОЖЖЕЙ Yarrowia lipolytica - ПРОДУЦЕНТ ЛИПАЗЫ
EP2327776A1 (en) 2009-11-30 2011-06-01 Institut National De La Recherche Agronomique Method for the production of Very Long Chain Fatty Acids (VLCFA) by fermentation with a recombinant Yarrowia sp
US20130295605A1 (en) * 2010-05-28 2013-11-07 Csir Method for producing a polypeptide in yarrowia lipolytica
CN102533692A (zh) * 2010-12-08 2012-07-04 刘刚 一种酸性条件下具有降解脂肪能力的脂肪酶
CN106754446B (zh) * 2016-10-31 2020-05-19 华中科技大学 一种转化解脂耶氏酵母及其构建方法与应用
EP4501403A3 (en) 2019-08-30 2025-05-14 Société des Produits Nestlé S.A. Engineered lipase variants
FR3111559A1 (fr) * 2020-06-18 2021-12-24 Azurrx Biopharma, Inc. Formulations non porcines et leurs procédés
KR102510688B1 (ko) 2022-06-15 2023-03-17 아스티스(주) 야로위아 리폴리티카 변이균주 및 이를 포함하는 기능성 화장료 조성물
CN117586900B (zh) * 2023-11-23 2024-07-26 中国海洋大学 表达脂肪酶OUC-Sb-lip2的重组解脂耶氏酵母菌及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795771A (en) * 1993-07-01 1998-08-18 Merck & Co., Inc. Culture medium for Saccharomyces cerevisiae
AU2000244105A1 (en) * 2000-04-28 2001-11-12 Laboratoires Mayoly Spindler Cloning and expressing an acid-resistant extracellular lipase of yarrowia lipolytica
EP2298872A3 (en) * 2004-09-30 2011-08-10 Novozymes A/S Polypeptides having lipase activity and polynucleotides encoding same

Also Published As

Publication number Publication date
CN101506359A (zh) 2009-08-12
NO20085119L (no) 2009-03-02
NZ573601A (en) 2011-12-22
BRPI0621829B1 (pt) 2018-04-10
PL2035556T3 (pl) 2015-12-31
EP2035556A1 (fr) 2009-03-18
DK2035556T3 (en) 2015-11-09
US20130052180A1 (en) 2013-02-28
EA200970012A1 (ru) 2009-10-30
BRPI0621829A2 (pt) 2011-12-20
JP5592648B2 (ja) 2014-09-17
JP2009539395A (ja) 2009-11-19
CR10515A (es) 2009-04-21
CU24009B1 (es) 2014-06-27
BRPI0621829B8 (pt) 2021-05-25
PT2035556E (pt) 2015-11-04
CA2657949A1 (fr) 2007-12-21
US8834867B2 (en) 2014-09-16
CA2657949C (fr) 2014-07-15
WO2007144475A1 (fr) 2007-12-21
AU2006344466B2 (en) 2013-08-15
EP2035556B1 (fr) 2015-07-29
UA97246C2 (ru) 2012-01-25
KR20090029808A (ko) 2009-03-23
ECSP099057A (es) 2009-08-28
ES2550537T3 (es) 2015-11-10
KR101497159B1 (ko) 2015-03-03
EA017963B1 (ru) 2013-04-30
SI2035556T1 (sl) 2015-12-31
US20120003716A1 (en) 2012-01-05
HK1129702A1 (zh) 2009-12-04
US8334130B2 (en) 2012-12-18
MX2008015978A (es) 2009-03-26
NO342291B1 (no) 2018-04-30
CN101506359B (zh) 2013-09-04
AU2006344466A1 (en) 2007-12-21

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