CU20190083A7 - Método mejorado para la producción de altos niveles de crm197 - Google Patents

Método mejorado para la producción de altos niveles de crm197

Info

Publication number
CU20190083A7
CU20190083A7 CU2019000083A CU20190083A CU20190083A7 CU 20190083 A7 CU20190083 A7 CU 20190083A7 CU 2019000083 A CU2019000083 A CU 2019000083A CU 20190083 A CU20190083 A CU 20190083A CU 20190083 A7 CU20190083 A7 CU 20190083A7
Authority
CU
Cuba
Prior art keywords
sub
improved method
crm197
high level
level production
Prior art date
Application number
CU2019000083A
Other languages
English (en)
Other versions
CU24668B1 (es
Inventor
Deviprasanna Chakka
Mahima Datla
Mandar Shirish Dixit
Narender Dev Mantena
Balamurali Masilamani
Ramesh Venkat Matur
Rajan Sriraman
Satyam Naidu Sureddi
Original Assignee
Biological E Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biological E Ltd filed Critical Biological E Ltd
Publication of CU20190083A7 publication Critical patent/CU20190083A7/es
Publication of CU24668B1 publication Critical patent/CU24668B1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/05—Actinobacteria, e.g. Actinomyces, Streptomyces, Nocardia, Bifidobacterium, Gardnerella, Corynebacterium; Propionibacterium
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/385—Haptens or antigens, bound to carriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Forging (AREA)
  • Manufacture Of Electron Tubes, Discharge Lamp Vessels, Lead-In Wires, And The Like (AREA)
  • Peptides Or Proteins (AREA)

Abstract

<p>La presente invención proporciona un método mejorado para la producción de CRM<sub>197</sub> con alto rendimiento mediante el uso de la cepa de Corynebacterium diphtheria modificada genéticamente que tiene un número incrementado de copias del gen de CRM<sub>197</sub>, en donde el método comprende crecer la cepa en un medio libre de componentes derivados de animales con uno o más aminoácidos.</p>
CU2019000083A 2017-04-22 2018-04-19 Método para la producción de alto nivel de crm197 a partir de una cepa genéticamente modificada de corynebacterium diphtheriae CU24668B1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN201741014335 2017-04-22
PCT/IN2018/050235 WO2018193475A2 (en) 2017-04-22 2018-04-19 An improved method for high level production of crm

Publications (2)

Publication Number Publication Date
CU20190083A7 true CU20190083A7 (es) 2020-10-20
CU24668B1 CU24668B1 (es) 2023-07-12

Family

ID=63678648

Family Applications (1)

Application Number Title Priority Date Filing Date
CU2019000083A CU24668B1 (es) 2017-04-22 2018-04-19 Método para la producción de alto nivel de crm197 a partir de una cepa genéticamente modificada de corynebacterium diphtheriae

Country Status (31)

Country Link
US (1) US11098089B2 (es)
EP (1) EP3612553B1 (es)
JP (1) JP7161493B2 (es)
KR (1) KR102637436B1 (es)
CN (1) CN110741013B (es)
AU (1) AU2018255959B2 (es)
BR (1) BR112019022093A2 (es)
CL (1) CL2019003003A1 (es)
CO (1) CO2019011643A2 (es)
CU (1) CU24668B1 (es)
CY (1) CY1125525T1 (es)
DK (1) DK3612553T3 (es)
EA (1) EA201992527A1 (es)
ES (1) ES2926535T3 (es)
HR (1) HRP20221060T1 (es)
HU (1) HUE059629T2 (es)
IL (1) IL270075B2 (es)
LT (1) LT3612553T (es)
MX (1) MX2019012563A (es)
MY (1) MY194242A (es)
PH (1) PH12019550214A1 (es)
PL (1) PL3612553T3 (es)
PT (1) PT3612553T (es)
RS (1) RS63551B1 (es)
SA (1) SA519410306B1 (es)
SG (1) SG11201909765XA (es)
SI (1) SI3612553T1 (es)
SM (1) SMT202200356T1 (es)
UA (1) UA127497C2 (es)
WO (1) WO2018193475A2 (es)
ZA (1) ZA201906270B (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2021003358A (es) 2018-09-23 2021-05-27 Biological E Ltd Polisacaridos capsulares purificados de streptococcus pneumoniae.
CN113755380A (zh) * 2021-09-23 2021-12-07 艾美卫信生物药业（浙江）有限公司 一种发酵生产白喉毒素crm197蛋白的菌种培养基、配制方法以及菌种复苏培养方法
CN115058474B (zh) * 2022-05-23 2025-02-14 艾美坚持生物制药有限公司 一种提高crm197蛋白产量的发酵培养方法
USD1055994S1 (en) 2023-01-02 2024-12-31 Samsung Electronics Co., Ltd. Refrigerator
CN119716089B (zh) * 2024-12-27 2025-12-09 浙江毓昌生物技术有限公司 一种基于高表达的crm197蛋白特异性中和检测方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE280235T1 (de) 1993-03-05 2004-11-15 Wyeth Corp Plasmid zur herstellung von crm-protein und diphtherie-toxin
DE69838383T2 (de) 1997-05-28 2008-06-05 Novartis Vaccines And Diagnostics S.R.L. Kulturmedium mit sojabohnenextrakt als aminosäuren-quelle und ohne proteinkomplexe tierischen ursprungs
US20040087020A1 (en) 1997-05-28 2004-05-06 Chiron S.P.A. Culture medium with yeast or soy bean extract as amino acid source and no protein complexes of animal origin
GB9904582D0 (en) 1999-02-26 1999-04-21 Nycomed Imaging As Process
US20110097359A1 (en) 2003-12-12 2011-04-28 Tim Lee Production of diphtheria toxin
GB0505996D0 (en) 2005-03-23 2005-04-27 Glaxosmithkline Biolog Sa Fermentation process
AU2010201410B2 (en) 2010-03-30 2015-04-30 Pelican Technology Holdings, Inc. High level expression of recombinant CRM197
GB2495341B (en) 2011-11-11 2013-09-18 Novartis Ag Fermentation methods and their products
FR2992656B1 (fr) * 2012-07-02 2016-10-07 Sanofi Pasteur Procede de production d'antigenes haemophilus influenzae type b
ITMI20130142A1 (it) * 2013-01-31 2014-08-01 Biosynth Srl Vaccini glicoconiugati comprendenti unita' di base di un costrutto molecolare esprimente epitopi multipli incorporati
WO2015134402A1 (en) * 2014-03-03 2015-09-11 Scarab Genomics, Llc Enhanced production of recombinant crm197 in e. coli

Also Published As

Publication number Publication date
CU24668B1 (es) 2023-07-12
CL2019003003A1 (es) 2020-02-14
US11098089B2 (en) 2021-08-24
BR112019022093A2 (pt) 2020-05-05
AU2018255959A1 (en) 2019-11-14
WO2018193475A2 (en) 2018-10-25
HRP20221060T1 (hr) 2022-11-11
IL270075A (es) 2019-12-31
CY1125525T1 (el) 2026-02-25
AU2018255959A8 (en) 2022-02-17
KR102637436B1 (ko) 2024-02-15
CN110741013B (zh) 2023-08-08
EP3612553A2 (en) 2020-02-26
LT3612553T (lt) 2022-09-26
EP3612553B1 (en) 2022-06-08
MY194242A (en) 2022-11-24
ZA201906270B (en) 2020-07-29
JP2020517274A (ja) 2020-06-18
IL270075B1 (en) 2024-02-01
PH12019550214A1 (en) 2020-09-14
SA519410306B1 (ar) 2022-02-02
PT3612553T (pt) 2022-09-08
PL3612553T3 (pl) 2022-11-07
AU2018255959B2 (en) 2022-02-17
SG11201909765XA (en) 2019-11-28
US20200277341A1 (en) 2020-09-03
CO2019011643A2 (es) 2020-01-17
ES2926535T3 (es) 2022-10-26
DK3612553T3 (da) 2022-08-29
EA201992527A1 (ru) 2020-02-28
HUE059629T2 (hu) 2022-12-28
CN110741013A (zh) 2020-01-31
WO2018193475A3 (en) 2018-12-27
SI3612553T1 (sl) 2022-11-30
MX2019012563A (es) 2019-12-02
RS63551B1 (sr) 2022-09-30
IL270075B2 (en) 2024-06-01
JP7161493B2 (ja) 2022-10-26
SMT202200356T1 (it) 2022-11-18
CA3060723A1 (en) 2018-10-25
KR20200010218A (ko) 2020-01-30
NZ758483A (en) 2024-10-25
UA127497C2 (uk) 2023-09-13

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