CU22277A1 - Procedimiento para el aislamiento y expresion de un gen codificante para estreptoquinasa,secuencia nucleotidica obtenida,adn recombinantes y microorganismos transformados - Google Patents

Procedimiento para el aislamiento y expresion de un gen codificante para estreptoquinasa,secuencia nucleotidica obtenida,adn recombinantes y microorganismos transformados

Info

Publication number
CU22277A1
CU22277A1 CU9090A CU1990090A CU22277A1 CU 22277 A1 CU22277 A1 CU 22277A1 CU 9090 A CU9090 A CU 9090A CU 1990090 A CU1990090 A CU 1990090A CU 22277 A1 CU22277 A1 CU 22277A1
Authority
CU
Cuba
Prior art keywords
streptokinase
expression
isolation
gene
organisms
Prior art date
Application number
CU9090A
Other languages
English (en)
Inventor
Garcia Mario Pablo Estrada
Felipe Aimee Perez
Chaplen Roger Rubiera
Doce Ricardo Serrano
Marrero Luciano Francisco Hernandez
Collazo Pedro Rodriguez
Ramirez Anaisel Castro
Munoz Emilio Amable Munoz
Martinez Walfrido Bravo
Somavilla Magalys Campos
Fernandez Alicia Pedraza
Original Assignee
Cigb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cigb filed Critical Cigb
Priority to CU9090A priority Critical patent/CU22277A1/es
Priority to ES90201930T priority patent/ES2081909T3/es
Priority to AT90201930T priority patent/ATE130369T1/de
Priority to EP90201930A priority patent/EP0489201B1/en
Priority to DK90201930.6T priority patent/DK0489201T3/da
Priority to DE69023655T priority patent/DE69023655T2/de
Priority to JP02201600A priority patent/JP3127298B2/ja
Priority to US07/703,778 priority patent/US5296366A/en
Priority to AU78101/91A priority patent/AU644657B2/en
Publication of CU22277A1 publication Critical patent/CU22277A1/es
Priority to GR950403496T priority patent/GR3018368T3/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C07K14/3153—Streptokinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Mycology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE RELACIONA CON LA RAMA DE LA BIOTECNOLOGIA Y LA INGENIERIA GENETICA Y EN OBTENIDA POR ESTA VIA PUEDE SER EMPLEADA EN MEDICINA CLINICA COMO AGENTE TERAPEUTICO, EN EL TRATAMIENTO DE ENFERMEDADES COMO BLOQUEOS TROMBOEMBOLICOS, INCLUYENDO TROMBOSIS CORONARIA. PARTICULAR CON UNA NUEVA SECUENCIA NUCLEOTIDICA QUE CODIFICA PARA UNA ESTREPTOQUINASA, ASI COMO LOS ADN RECOMBINANTES OBTENIDOS A PARTIR DE ELLA QUE SON EMPLEADOS PARA LA TRANSFORMACION DE DIFERENTES ORGANISMOS HOSPEDEROS. EL OBJETIVO TECNICO QUE SE PERSIGUE CON LA SOLUCION PROPUESTA, ES PRODUCIR LA PROTEINA EN CANTIDADES COMERCIALMENTE UTILES, POR VIA RECOMBINANTE, CON LA POSIBILIDAD DE EMPLEAR DE FORMA ALTERNATIVA HOSPEDEROS DIFERENTES PARA OBTENER UNA ESTREPTOQUINASA DE GRAN CALIDAD CAPAZ DE SER EMPLEADA COMO AGENTE TROMBOLITICO EN EL TRATAMIENTO DE DIFERENTES ENFERMEDADES. ESTA INVENCION SE BASA EN EL AISLAMIENTO DE UN NUEVO GEN QUE CODIFICA PARA LA ESTREPTOQUINASA PROVENIENTE DE STREPTOCOCCUS EQUISIMILIS DEL GRUPO C (CEPA ATCC-9542) Y SU CLONAJE Y EXPRESION EN HOSPEDEROS PROCARIOTICOS (E.COLI) Y EUCARIOTICOS (PICHIA PASTORIS), POR LO QUE LA MISMA INCLUYE LOS VEHICULOS DE EXPRESION QUE CONTIENEN LAS SEC UENCIAS GENICAS DE DICHO GEN, ASI COMO LOS MICROORGANISMOS TRANSFORMADOS CON ESTOS VECTORES CAPACES DE PRODUCIR ESTREPTOQUINASA. LA PROTEINA\!
CU9090A 1990-05-23 1990-05-23 Procedimiento para el aislamiento y expresion de un gen codificante para estreptoquinasa,secuencia nucleotidica obtenida,adn recombinantes y microorganismos transformados CU22277A1 (es)

Priority Applications (10)

Application Number Priority Date Filing Date Title
CU9090A CU22277A1 (es) 1990-05-23 1990-05-23 Procedimiento para el aislamiento y expresion de un gen codificante para estreptoquinasa,secuencia nucleotidica obtenida,adn recombinantes y microorganismos transformados
ES90201930T ES2081909T3 (es) 1990-05-23 1990-07-17 Metodo para el aislamiento y la expresion de un gen que codifica estreptoquinasa, secuencia de nucleotidos obtenida, adn recombinante y microorganismos transformados.
AT90201930T ATE130369T1 (de) 1990-05-23 1990-07-17 Verfahren zur isolierung und expression eines für streptokinase kodierenden gens, nukleotidsequenz, rekombinante dna und transformierter mikroorganismus.
EP90201930A EP0489201B1 (en) 1990-05-23 1990-07-17 Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant DNA and transformed microorganisms
DK90201930.6T DK0489201T3 (da) 1990-05-23 1990-07-17 Fremgangsmåde til isolation og expression af et gen, som koder for streptokinase, de opnåede nucleot idsekvenser, recombinant DNA og transformerede mikroorganismer
DE69023655T DE69023655T2 (de) 1990-05-23 1990-07-17 Verfahren zur Isolierung und Expression eines für Streptokinase kodierenden Gens, Nukleotidsequenz, rekombinante DNA und transformierter Mikroorganismus.
JP02201600A JP3127298B2 (ja) 1990-05-23 1990-07-31 ストレプトキナーゼをコードする遺伝子の単離および発現方法、得られるヌクレオチド配列、組換体ｄｎａおよび形質転換微生物
US07/703,778 US5296366A (en) 1990-05-23 1991-05-22 Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant DNA and transformed microorgnaisms
AU78101/91A AU644657B2 (en) 1990-05-23 1991-05-31 Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant dna and transformed microorganisms
GR950403496T GR3018368T3 (en) 1990-05-23 1995-12-12 Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant DNA and transformed microorganisms.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU9090A CU22277A1 (es) 1990-05-23 1990-05-23 Procedimiento para el aislamiento y expresion de un gen codificante para estreptoquinasa,secuencia nucleotidica obtenida,adn recombinantes y microorganismos transformados

Publications (1)

Publication Number Publication Date
CU22277A1 true CU22277A1 (es) 1995-01-31

Family

ID=5459333

Family Applications (1)

Application Number Title Priority Date Filing Date
CU9090A CU22277A1 (es) 1990-05-23 1990-05-23 Procedimiento para el aislamiento y expresion de un gen codificante para estreptoquinasa,secuencia nucleotidica obtenida,adn recombinantes y microorganismos transformados

Country Status (10)

Country Link
US (1) US5296366A (es)
EP (1) EP0489201B1 (es)
JP (1) JP3127298B2 (es)
AT (1) ATE130369T1 (es)
AU (1) AU644657B2 (es)
CU (1) CU22277A1 (es)
DE (1) DE69023655T2 (es)
DK (1) DK0489201T3 (es)
ES (1) ES2081909T3 (es)
GR (1) GR3018368T3 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1035193C (zh) * 1994-04-04 1997-06-18 上海医科大学 一种制备重组链激酶的方法
CU22732A1 (es) * 1998-08-14 2002-02-28 Ct Ingenieria Genetica Biotech Mutantes de estreptoquinasa
US7105327B1 (en) 2001-06-15 2006-09-12 Bharat Biotech International, Ltd. Recombinant streptokinase
AU2003295182B9 (en) * 2003-12-23 2009-12-17 Council Of Scientific And Industrial Research Process for producing streptokinase using genetically modified Escherichia coli

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA872534B (en) * 1986-05-08 1987-11-25 Phillips Petroleum Co Yeast production of streptokinase
JPH07110259B2 (ja) * 1988-06-13 1995-11-29 松下電器産業株式会社 自動炊飯器
GB8927722D0 (en) * 1989-12-07 1990-02-07 British Bio Technology Proteins and nucleic acids
JP3304369B2 (ja) * 1991-11-11 2002-07-22 井関農機株式会社 洗米炊飯装置
JPH0819478A (ja) * 1994-07-11 1996-01-23 Iseki & Co Ltd 全自動炊飯装置
JP2000126034A (ja) * 1998-10-28 2000-05-09 Daiwa Seiko Inc 洗米供給方法及び洗米供給装置
JP2001128851A (ja) * 1999-11-08 2001-05-15 Fujimak Corp 自動炊飯機
JP3852339B2 (ja) * 2002-01-18 2006-11-29 井関農機株式会社 洗米炊飯機
JP4258362B2 (ja) * 2003-12-08 2009-04-30 井関農機株式会社 洗米処理装置
KR200402213Y1 (ko) * 2005-09-13 2005-11-29 김성곤 무인 자동 취사장치
JP2008029933A (ja) * 2006-07-27 2008-02-14 Kubota Corp 洗米方法及び洗米機

Also Published As

Publication number Publication date
AU7810191A (en) 1991-11-28
JP3127298B2 (ja) 2001-01-22
AU644657B2 (en) 1993-12-16
DK0489201T3 (da) 1996-03-11
EP0489201B1 (en) 1995-11-15
DE69023655T2 (de) 1996-05-02
ES2081909T3 (es) 1996-03-16
GR3018368T3 (en) 1996-03-31
JPH0430794A (ja) 1992-02-03
US5296366A (en) 1994-03-22
EP0489201A1 (en) 1992-06-10
ATE130369T1 (de) 1995-12-15
DE69023655D1 (de) 1995-12-21

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