ES2081909T3 - Metodo para el aislamiento y la expresion de un gen que codifica estreptoquinasa, secuencia de nucleotidos obtenida, adn recombinante y microorganismos transformados. - Google Patents
Metodo para el aislamiento y la expresion de un gen que codifica estreptoquinasa, secuencia de nucleotidos obtenida, adn recombinante y microorganismos transformados.Info
- Publication number
- ES2081909T3 ES2081909T3 ES90201930T ES90201930T ES2081909T3 ES 2081909 T3 ES2081909 T3 ES 2081909T3 ES 90201930 T ES90201930 T ES 90201930T ES 90201930 T ES90201930 T ES 90201930T ES 2081909 T3 ES2081909 T3 ES 2081909T3
- Authority
- ES
- Spain
- Prior art keywords
- streptokinase
- codes
- expression
- gene
- isolation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108010023197 Streptokinase Proteins 0.000 title abstract 5
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 229960005202 streptokinase Drugs 0.000 title abstract 5
- 108020004511 Recombinant DNA Proteins 0.000 title abstract 2
- 238000002955 isolation Methods 0.000 title abstract 2
- 244000005700 microbiome Species 0.000 title abstract 2
- 239000002773 nucleotide Substances 0.000 title abstract 2
- 238000000034 method Methods 0.000 title 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 2
- 208000035475 disorder Diseases 0.000 abstract 2
- 239000003814 drug Substances 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 206010011091 Coronary artery thrombosis Diseases 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 241000235058 Komagataella pastoris Species 0.000 abstract 1
- 241000264435 Streptococcus dysgalactiae subsp. equisimilis Species 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 208000002528 coronary thrombosis Diseases 0.000 abstract 1
- 239000003527 fibrinolytic agent Substances 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 238000010353 genetic engineering Methods 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 238000010188 recombinant method Methods 0.000 abstract 1
- 229940124597 therapeutic agent Drugs 0.000 abstract 1
- 230000009424 thromboembolic effect Effects 0.000 abstract 1
- 229960000103 thrombolytic agent Drugs 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
- C07K14/3153—Streptokinase
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
LA PRESENTE INVENCION SE REFIERE AL CAMPO DE LA BIOTECNOLOGIA, E INGENIERIA GENETICA, Y PARTICULARMENTE A UNA NOVISIMA SECUENCIA DE NUCLEOTIDOS QUE CODIFICAN UNA ESTREPTOKINASA, COMO ASI MISMO, EL REASOCIANTE DE DNA, OBTENIDO DE ELLO, UTILIZANDOSE PARA LA TRANSFORMACION DE DIFERENTES ORGANISMOS ENDOGENOS. EL OBJETIVO TECNICO QUE SE PERSIGUE CON LA SOLUCION PROPUESTA, ES LA PRODUCCION DE PROTEINAS EN CANTIDADES CONVENIENTES PARA SU COMERCIALIZACION, MEDIANTE EL METODO DE REASOCIACION, OFRECIENDO LA POSIBILIDAD DEL USO ALTERNATIVO DE DIFERENTES INDIVIDUOS ENDOGENOS, A FIN DE CONSEGUIR ESTREPTOKINASA DE EXCELENTE CALIDAD, SUSCEPTIBLE DE SER UTILIZADA COMO AGENTE TROMBOLITICO EN EL TRATAMIENTO DE DIFERENTES TRASTORNOS. LA PRESENTE INVENCION, ESTA BASADA EN EL AISLAMIENTO DE UN NUEVO GENE QUE CODIFICA ESTREPTOKINASA DEL STREPTOCOCCUS EQUISIMILIS DEL TIPO C, (CADENA ATCC-9542), Y EL CLONISMO Y MANIFESTACION DEL MISMO EN PATRONES (E. COLI), Y EUKARIOTICO (PICHIA PASTORIS), PARA LO CUAL, SE INCLUYEN LOS VEHICULOS DE LOCALIZACION QUE CONTIENEN LAS SECUENCIAS GENETICAS DE DICHO GENE, ASI COMO TAMBIEN, LOS MICROORGANISMOS MUTADOS EN DICHOS VECTORES, CON CAPACIDAD PARA PRODUCIR ESTREPTOKINASA. LA PROTEINA ASI OBTENIDA, PUEDE SER UTILIZADA EN MEDICINA CLINICA, ASI COMO AGENTE TERAPEUTICO EN EL TRATAMIENTO DE DESORDENES, TALES COMO OBSTRUCCION TROMBOEMBOLICA, INCLUYENDO LA TROMBOSIS CORONARIA.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CU9090A CU22277A1 (es) | 1990-05-23 | 1990-05-23 | Procedimiento para el aislamiento y expresion de un gen codificante para estreptoquinasa,secuencia nucleotidica obtenida,adn recombinantes y microorganismos transformados |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2081909T3 true ES2081909T3 (es) | 1996-03-16 |
Family
ID=5459333
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES90201930T Expired - Lifetime ES2081909T3 (es) | 1990-05-23 | 1990-07-17 | Metodo para el aislamiento y la expresion de un gen que codifica estreptoquinasa, secuencia de nucleotidos obtenida, adn recombinante y microorganismos transformados. |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US5296366A (es) |
| EP (1) | EP0489201B1 (es) |
| JP (1) | JP3127298B2 (es) |
| AT (1) | ATE130369T1 (es) |
| AU (1) | AU644657B2 (es) |
| CU (1) | CU22277A1 (es) |
| DE (1) | DE69023655T2 (es) |
| DK (1) | DK0489201T3 (es) |
| ES (1) | ES2081909T3 (es) |
| GR (1) | GR3018368T3 (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1035193C (zh) * | 1994-04-04 | 1997-06-18 | 上海医科大学 | 一种制备重组链激酶的方法 |
| CU22732A1 (es) * | 1998-08-14 | 2002-02-28 | Ct Ingenieria Genetica Biotech | Mutantes de estreptoquinasa |
| US7105327B1 (en) | 2001-06-15 | 2006-09-12 | Bharat Biotech International, Ltd. | Recombinant streptokinase |
| AU2003295182B9 (en) * | 2003-12-23 | 2009-12-17 | Council Of Scientific And Industrial Research | Process for producing streptokinase using genetically modified Escherichia coli |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA872534B (en) * | 1986-05-08 | 1987-11-25 | Phillips Petroleum Co | Yeast production of streptokinase |
| JPH07110259B2 (ja) * | 1988-06-13 | 1995-11-29 | 松下電器産業株式会社 | 自動炊飯器 |
| GB8927722D0 (en) * | 1989-12-07 | 1990-02-07 | British Bio Technology | Proteins and nucleic acids |
| JP3304369B2 (ja) * | 1991-11-11 | 2002-07-22 | 井関農機株式会社 | 洗米炊飯装置 |
| JPH0819478A (ja) * | 1994-07-11 | 1996-01-23 | Iseki & Co Ltd | 全自動炊飯装置 |
| JP2000126034A (ja) * | 1998-10-28 | 2000-05-09 | Daiwa Seiko Inc | 洗米供給方法及び洗米供給装置 |
| JP2001128851A (ja) * | 1999-11-08 | 2001-05-15 | Fujimak Corp | 自動炊飯機 |
| JP3852339B2 (ja) * | 2002-01-18 | 2006-11-29 | 井関農機株式会社 | 洗米炊飯機 |
| JP4258362B2 (ja) * | 2003-12-08 | 2009-04-30 | 井関農機株式会社 | 洗米処理装置 |
| KR200402213Y1 (ko) * | 2005-09-13 | 2005-11-29 | 김성곤 | 무인 자동 취사장치 |
| JP2008029933A (ja) * | 2006-07-27 | 2008-02-14 | Kubota Corp | 洗米方法及び洗米機 |
-
1990
- 1990-05-23 CU CU9090A patent/CU22277A1/es unknown
- 1990-07-17 EP EP90201930A patent/EP0489201B1/en not_active Expired - Lifetime
- 1990-07-17 DK DK90201930.6T patent/DK0489201T3/da not_active Application Discontinuation
- 1990-07-17 DE DE69023655T patent/DE69023655T2/de not_active Expired - Fee Related
- 1990-07-17 AT AT90201930T patent/ATE130369T1/de not_active IP Right Cessation
- 1990-07-17 ES ES90201930T patent/ES2081909T3/es not_active Expired - Lifetime
- 1990-07-31 JP JP02201600A patent/JP3127298B2/ja not_active Expired - Fee Related
-
1991
- 1991-05-22 US US07/703,778 patent/US5296366A/en not_active Expired - Fee Related
- 1991-05-31 AU AU78101/91A patent/AU644657B2/en not_active Ceased
-
1995
- 1995-12-12 GR GR950403496T patent/GR3018368T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE69023655D1 (de) | 1995-12-21 |
| JPH0430794A (ja) | 1992-02-03 |
| US5296366A (en) | 1994-03-22 |
| ATE130369T1 (de) | 1995-12-15 |
| GR3018368T3 (en) | 1996-03-31 |
| EP0489201B1 (en) | 1995-11-15 |
| AU7810191A (en) | 1991-11-28 |
| DE69023655T2 (de) | 1996-05-02 |
| JP3127298B2 (ja) | 2001-01-22 |
| CU22277A1 (es) | 1995-01-31 |
| DK0489201T3 (da) | 1996-03-11 |
| EP0489201A1 (en) | 1992-06-10 |
| AU644657B2 (en) | 1993-12-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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