CU22808A1 - Composición y método para la detección y recuento diferenciado y temprano de organismos microscópicos gram-negativos - Google Patents
Composición y método para la detección y recuento diferenciado y temprano de organismos microscópicos gram-negativosInfo
- Publication number
- CU22808A1 CU22808A1 CU20000083A CU20000083A CU22808A1 CU 22808 A1 CU22808 A1 CU 22808A1 CU 20000083 A CU20000083 A CU 20000083A CU 20000083 A CU20000083 A CU 20000083A CU 22808 A1 CU22808 A1 CU 22808A1
- Authority
- CU
- Cuba
- Prior art keywords
- colonies
- composition
- mixture
- detection
- gram
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title abstract 8
- 238000001514 detection method Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 239000000126 substance Substances 0.000 abstract 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 2
- 230000004069 differentiation Effects 0.000 abstract 2
- 244000005700 microbiome Species 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 241000588923 Citrobacter Species 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 241000588748 Klebsiella Species 0.000 abstract 1
- 241000588769 Proteus <enterobacteria> Species 0.000 abstract 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 abstract 1
- 241000607142 Salmonella Species 0.000 abstract 1
- 241000293871 Salmonella enterica subsp. enterica serovar Typhi Species 0.000 abstract 1
- 241001467018 Typhis Species 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
- 229910052757 nitrogen Inorganic materials 0.000 abstract 1
- 230000019612 pigmentation Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/10—Enterobacteria
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
La presente invención se relaciona con la Microbiología y particularmente con una composición y un método empleados para la detección, identificación, diferenciación y recuento temprano de organismos microscópicos, concretamente microorganismos Gram-negativos. La composición de la presente invención se caracteriza por contener una mezcla de sustancias de origen proteico con un contenido de nitrógeno total de 9 a 20% y en una relación entre 2:1 a24: 1 con respecto al contenido de inhibidores de los organismos Gram-positivos, y comprende además una mezcla de sustancias orgánicas e inorgánicas que posibilitan la diferenciación de los mismos, encontrándose esta mezcla en una relación entre 0.5 : 1 a 2 : 1 con respecto a la mezcla de sustancias de origen proteico. La referida composición permite la detección y recuento diferenciado de E. Coli y otros organismos coliformes debido a la coloración azul - verdosa de las colonias de estos microorganismos sobre el fondo naranja del medio; la de Salmonella no typhi por el color rojo del centro de las colonias sobre fondo rosado del medio ; la de Salmonella typhi y Proteus por la transparencia de las colonias; la de Citrobacter y Klebsiella por la coloración violeta de las colonias sobre el fondo de rosado a naranja del medio y la de Pseudomonas aeruginosa por la coloración naranja con centro más oscuro de la colonia, tomando pigmentación verdosa a partir de las 24 horas y presentando fluorescencia verdosa bajo luz ultravioleta.
Priority Applications (13)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CU20000083A CU22808A1 (es) | 2000-04-18 | 2000-04-18 | Composición y método para la detección y recuento diferenciado y temprano de organismos microscópicos gram-negativos |
| EP01925277A EP1196625B1 (en) | 2000-04-18 | 2001-04-17 | Composition and method for detecting and early and differentiated counting of gram-negative microorganisms |
| US10/018,738 US20030044882A1 (en) | 2000-04-18 | 2001-04-17 | Composition and method for detecting and early and differentiated counting of gram-negative microorganisms |
| BR0106067-8A BR0106067A (pt) | 2000-04-18 | 2001-04-17 | Composição e método para detecção e contagem de forma diferenciada e antecipada de microorganismos gram-negativos |
| PCT/CU2001/000002 WO2001081615A1 (es) | 2000-04-18 | 2001-04-17 | Composicion y metodo para la deteccion y el recuento temprano y diferenciado de microorganismos gram-negativos |
| MXPA01012869A MXPA01012869A (es) | 2000-04-18 | 2001-04-17 | Composicion y metodo para la deteccion y el recuentro temprano y diferenciado de microorganismos gram-negativos. |
| DE60122081T DE60122081T8 (de) | 2000-04-18 | 2001-04-17 | Zusammensetzung und verfahren zum nachweis und zum frühen und differenzierten zählen von gramnegativen mikroorganismen |
| CA002377242A CA2377242A1 (en) | 2000-04-18 | 2001-04-17 | Composition and method for detecting and early and differentiated counting of gram-negative microorganisms |
| AT01925277T ATE335837T1 (de) | 2000-04-18 | 2001-04-17 | Zusammensetzung und verfahren zum nachweis und zum frühen und differenzierten zählen von gramnegativen mikroorganismen |
| JP2001578686A JP2003530890A (ja) | 2000-04-18 | 2001-04-17 | グラム陰性微生物の検出および早期区別的菌数測定用の方法および組成物 |
| RU2002100820/13A RU2264466C2 (ru) | 2000-04-18 | 2001-04-17 | Композиция и способ обнаружения и раннего и дифференцированного подсчета грамотрицательных микроорганизмов |
| GT200100060A GT200100060A (es) | 2000-04-18 | 2001-04-17 | Composicion y metodo para la deteccion y el recuento temprano y diferenciado de organismos microscopicos gram-negativos. |
| ARP010101815A AR028016A1 (es) | 2000-04-18 | 2001-04-18 | Composicion y metodo para la detenccion y el recuento temprano y diferenciado de organismos microscopicos gram-negaticos |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CU20000083A CU22808A1 (es) | 2000-04-18 | 2000-04-18 | Composición y método para la detección y recuento diferenciado y temprano de organismos microscópicos gram-negativos |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CU22808A1 true CU22808A1 (es) | 2005-06-24 |
Family
ID=40286955
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CU20000083A CU22808A1 (es) | 2000-04-18 | 2000-04-18 | Composición y método para la detección y recuento diferenciado y temprano de organismos microscópicos gram-negativos |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20030044882A1 (es) |
| EP (1) | EP1196625B1 (es) |
| JP (1) | JP2003530890A (es) |
| AR (1) | AR028016A1 (es) |
| AT (1) | ATE335837T1 (es) |
| BR (1) | BR0106067A (es) |
| CA (1) | CA2377242A1 (es) |
| CU (1) | CU22808A1 (es) |
| DE (1) | DE60122081T8 (es) |
| GT (1) | GT200100060A (es) |
| MX (1) | MXPA01012869A (es) |
| RU (1) | RU2264466C2 (es) |
| WO (1) | WO2001081615A1 (es) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090069743A1 (en) * | 2007-09-11 | 2009-03-12 | Baxter International Inc. | Infusion therapy sensor system |
| AU2011262289B2 (en) * | 2010-06-03 | 2014-03-20 | Basf Se | Detection and enumeration of microorganisms |
| CU24137B1 (es) * | 2012-03-30 | 2015-12-23 | Ct Nac Biopreparados | Método para la detección, recuperación, identificación y enumeración simultánea de microorganismos |
| RU2508399C1 (ru) * | 2012-07-17 | 2014-02-27 | Федеральное бюджетное учреждение науки Государственный научный центр прикладной микробиологии и биотехнологии (ФБУН ГНЦ ПМБ) | СУХАЯ ДИФФЕРЕНЦИАЛЬНО-ДИАГНОСТИЧЕСКАЯ ПИТАТЕЛЬНАЯ СРЕДА ДЛЯ ОБНАРУЖЕНИЯ И УЧЕТА E.coli И КОЛИФОРМНЫХ БАКТЕРИЙ |
| RU2508400C1 (ru) * | 2012-07-17 | 2014-02-27 | Федеральное бюджетное учреждение науки Государственный научный центр прикладной микробиологии и биотехнологии (ФБУН ГНЦ ПМБ) | СУХАЯ ХРОМОГЕННАЯ ПИТАТЕЛЬНАЯ СРЕДА ДЛЯ ОБНАРУЖЕНИЯ КОЛИФОРМНЫХ БАКТЕРИЙ И E.coli (ВАРИАНТЫ) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2649410B2 (fr) * | 1989-04-27 | 1994-08-19 | Eurec | Perfectionnement d'un milieu d'isolement pour l'identification de la bacterie salmonella |
| US5194374A (en) * | 1989-04-27 | 1993-03-16 | Eurec | Isolating medium for identifying the salmonella bacterium |
| US5723308A (en) * | 1993-05-14 | 1998-03-03 | Minnesota Mining And Manufacturing Company | Culture medium for rapid count of coliform bacteria |
| FR2708285B1 (fr) * | 1993-07-28 | 1995-10-20 | Rambach Alain | Procédé d'identification de microorganismes avec un milieu supplémenté en hydrate de carbone. |
| ES2079319B1 (es) * | 1994-05-16 | 1996-07-16 | Aguayo Jose Maria Garcia | Medio de cultivo bacteriologico y procedimiento para su preparacion. |
| FI98379C (fi) * | 1995-03-24 | 1997-06-10 | Orion Yhtymae Oy | Elatusaine ja menetelmä salmonellojen tunnistamiseksi |
-
2000
- 2000-04-18 CU CU20000083A patent/CU22808A1/es unknown
-
2001
- 2001-04-17 WO PCT/CU2001/000002 patent/WO2001081615A1/es not_active Ceased
- 2001-04-17 CA CA002377242A patent/CA2377242A1/en not_active Abandoned
- 2001-04-17 GT GT200100060A patent/GT200100060A/es unknown
- 2001-04-17 DE DE60122081T patent/DE60122081T8/de active Active
- 2001-04-17 AT AT01925277T patent/ATE335837T1/de not_active IP Right Cessation
- 2001-04-17 JP JP2001578686A patent/JP2003530890A/ja active Pending
- 2001-04-17 US US10/018,738 patent/US20030044882A1/en not_active Abandoned
- 2001-04-17 RU RU2002100820/13A patent/RU2264466C2/ru not_active IP Right Cessation
- 2001-04-17 BR BR0106067-8A patent/BR0106067A/pt not_active IP Right Cessation
- 2001-04-17 MX MXPA01012869A patent/MXPA01012869A/es active IP Right Grant
- 2001-04-17 EP EP01925277A patent/EP1196625B1/en not_active Expired - Lifetime
- 2001-04-18 AR ARP010101815A patent/AR028016A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| RU2264466C2 (ru) | 2005-11-20 |
| CA2377242A1 (en) | 2001-11-01 |
| AR028016A1 (es) | 2003-04-23 |
| EP1196625B1 (en) | 2006-08-09 |
| BR0106067A (pt) | 2002-04-30 |
| EP1196625A1 (en) | 2002-04-17 |
| US20030044882A1 (en) | 2003-03-06 |
| JP2003530890A (ja) | 2003-10-21 |
| DE60122081T8 (de) | 2007-10-11 |
| ATE335837T1 (de) | 2006-09-15 |
| DE60122081T2 (de) | 2007-03-08 |
| GT200100060A (es) | 2002-04-25 |
| DE60122081D1 (de) | 2006-09-21 |
| WO2001081615A1 (es) | 2001-11-01 |
| MXPA01012869A (es) | 2002-07-30 |
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