CZ20023642A3 - Geny virulence, proteiny a jejich použití - Google Patents

Description

Vynález se týká virulentních genů, proteinů a jejich použití. Přesněji, týká se genů a proteinů/peptidů získaných z Neisseria meningitidis a jejich použití při léčbě a screeningu léčiv.

Dosavadní stav techniky

Neisseria meningitidis je Gram-negativní bakteriální patogen, který se podílí na septickém šoku a bakteriální meningitidě. Tato bakterie je hlavní příčinou bakteriální meningitidy v rozvojových zemích a způsobuje rozsáhlou epidemii v Africe a Číně. V UK je Neisseria meningitidis hlavní příčinou smrti v dětství kromě dopravních nehod. Bakterie přirozeně inhibuje nosohltan pacienta a pak proniká do krevního oběhu a touto cestou způsobuje vážné septikemii nebo meningitidu. Ačkoli jsou současné antimikrobiální přípravky účinné při odstraňování bakterie z těla, úmrtnost na menigokokální septikemii je stále značná. Bylo by třeba poskytnout způsob léčby nebo prevence stavů vyvolaných Neisseria meningitidis, například imunizací.

Podstata vynálezu

Vynález vychází z objevu virulentních genů Neisseria meningitidis.

Podle prvního hlediska vynálezu je peptid podle vynálezu kódován operonem zahrnujícím kteroukoli nukleotidovou sekvenci zde uváděných jako SEQ ID NOS.

1, 3, 5, 7, 9, 11,13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91,

93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125,

127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157,

159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189,

191, 193, 195, 197, 199, 201, 203, 205, 207 u N. meningitidis nebo podobné molekuly s alespoň 40% sekvenční podobností nebo shodou na úrovni peptidu nebo nukleotidu u Gram-negativní bakterie nebo jejího funkčního fragmentu pro terapeutické nebo diagnostické použití.

Peptidy mohou mít více léčebných použití pro léčbu infekcí Neisseria včetně použití jako očkovacích látek pro profílaktickou aplikaci.

Podle dalšího hlediska polynukleotid kódující peptid uvedený výše, může být též použitelný pro léčbu a diagnostiku.

Podle třetího hlediska mohou být geny, které kódují peptidy, použity k přípravě oslabených mikroorganismů. Oslabené mikroorganismy většinou obsahují mutaci, která přerušuje expresi jednoho nebo více genů zde uváděných za účelem přípravy kmene, který není virulentní. Tyto mikroorganismy je též možné použít k léčbě nebo diagnostice.

Podle čtvrtého hlediska mohou být peptidy, geny a oslabené mikroorganismy podle vynálezu použity pro léčbu nebo prevenci stavů spojených s infekcí Neisseria nebo Gram-negativními bakteriemi.

Vynález je založen na objevu genů kódujících peptidy, které se podílejí na virulenci. Peptidy a geny podle vynálezu jsou proto použitelné pro přípravu terapeutických látek pro léčbu infekce. Je třeba pochopit, že odkazy týkající se terapie zahrnují též prevenci, například očkování. Ačkoli produkty podle vynálezu jsou primárně určeny pro léčbu infekcí člověka, veterinární aplikace jsou též zahrnuty v rozsahu vynálezu.

Vynález je popsán s odkazem na Neisseria meningitidis. Avšak všechny kmeny Neisseria a mnoho dalších Gram-negativních bakteriálních kmenů pravděpodobně obsahují podobné peptidy nebo proteiny obsahující aminokyselinovou sekvenci shodnou nebo podobnou se sekvencí zde uváděnou. Podobné organismy obsahující peptid zahrnují, ale nejsou omezeny na rody Salmonella, Enterobacter, Klebsiella, Shigella a Yersinia.

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Provedené experimenty identifikují virulentní geny podle vynálezu využívající kmen Β N. meningitidis. Shody byly hledány v databázi kmene A, avšak výhodné jsou proteiny a geny kmene B.

Výhodně, peptidy, které jsou použitelné z různých hledisek podle vynálezu mají větší než 40% podobnost s peptidy zde uváděnými. Výhodněji, peptidy mají vyšší než 60% sekvenční podobnost. Nejlépe, peptidy mají vyšší než 80% sekvenční podobnost, například 95% podobnost. S ohledem na polynukleotidové sekvence zde uváděné, odvozené polynukleotidy, které mohou být použitelné z různých hledisek podle vynálezu, mají větší než 40% shodu s uváděnými sekvencemi. Výhodněji, polynukleotidové sekvence mají větší než 60% sekvenční shodu. Nejlépe, polynukleotidové sekvence mají vyšší než 80% sekvenční shodu, například 95% shodu.

Pojmy „podobnost“ a „shoda“ jsou známy v dosavadním stavu techniky. Použití pojmu „shoda“ se týká sekvenčního srovnání založeném na shodě mezi odpovídajícími shodnými pozicemi ve srovnávaných sekvencích. Pojem „podobnost“ se týká srovnám mezi aminokyselinovými sekvencemi a zahrnuje nejen shodné aminokyseliny v odpovídajících pozicích, ale též funkční podobnost aminokyselin v odpovídajících pozicích. Podobnost mezi polypeptidovými sekvencemi tak kromě sekvenční podobnosti vypovídá o funkční podobnosti.

Míra shody mezi genovou sekvencí a míra shody nebo podobnost mezi aminokyselinovými sekvencemi lze vypočítat pomocí známých metod. Vzhledem k vynálezu běžně dostupné počítačové metody pro stanovení shody a podobnosti zahrnují BLASTP, BLASTIN a FASTA (Atschul a kol., J. Molec. Biol., 1990; 215:403-410), pogram BLASTX dostupný z NCBI a program Gap od Genetic Computer Group, Madison WI. Míry podobnosti a shody takto stanovované se získaly za použití programu Gap s omezením 12 a Gap omezující délkou 4 pro stanovení srovnání aminokyselinové sekvence a Gap omezením 50 a Gap omezující délkou 3 pro srovnání polynukleotidové sekvence.

Získáním kmenové charakteristiky podle vynálezu je možné použít genovou sekvenci pro vyhledání odvozených genů nebo peptidů u jiných mikroorganismů. Toto je možné provést vyhledáváním v existujících databázích, například EMBL nebo GenBank.

Peptidy nebo proteiny podle vynálezu mohou být přečištěné a izolované metodami známými v dosavadním stavu techniky. Přesněji, po identifikaci genové sekvence je možné použít rekombinantní techniky pro expresi genů ve vhodném hostiteli. Je možné odhalit aktivní fragmenty a odvozené molekuly, které mohou být použitelné pro léčbu. Například peptidy nebo jejich aktivní fragmenty mohou být použitelné jako antigenní determinanty v očkovací látce k vyvolání imunní reakce. Mohou být též použitelné při přípravě protilátek, pro pasivní imunizaci nebo diagnostické aplikace. Vhodné protilátky zahrnují monoklonální protilátky nebo jejich fragmenty včetně jednoduchého řetězce fragmentu Fv. Metody přípravy protilátek budou známé odborníkům v tomto oboru.

Aktivní fragmenty peptidů jsou takové, které zachovávají biologickou funkci peptidu. Například, pokud se použijí k vyvolání imunitní reakce, fragment musí mít dostatečnou velikost, aby protilátky vytvořené z fragmentu rozlišily mezi tímto a jinými peptidy bakteriálního mikroorganismu. Fragment má délku běžně alespoň 30 nukleotidů (10 aminokyselin), s výhodou 60 nukleotidů (20 aminokyselin) a nejlépe délku více než 90 nukleotidů (30 aminokyselin).

Je třeba si uvědomit, že kromě odvozených molekul jiných mikroorganismů vynález zahrnuje modifikace provedené u peptidů a polynukleotidů zde uvedených, které významně nemění biologickou funkci. Odborníkům v tomto oboru bude zřejmé, že degenerace genetického kódu může vést k polynukleotidů s minimálními změnami v bázích zde uváděných, které však přesto kódují shodný peptid. Komplementární polynukleotidy jsou též předmětem vynálezu. Konzervativní nahrazení na úrovni aminokyselin jsou též uvažována, to znamená různé kyselé nebo bazické aminokyseliny mohou být nahrazeny bez podstatné ztráty funkce.

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Příprava očkovacích látek založených na oslabených mikroorganismech je známá odborníkům v tomto oboru. Složení očkovací látky může být formulováno vhodnými nosiči nebo adjuvans, například kamencem, pokud je to nezbytné nebo žádané pro dosažení účinné imunizace proti infekci. Příprava formulace očkovací látky je známá odborníkům v tomto oboru. Oslabené mikroorganismy je možné připravit mutací, která porušuje expresi kteréhokoli uvedeného genu. Odborníkovi v tom to oboru jsou známy metody porušení exprese určitých genů. Techniky, které je možné použít, zahrnují inaktivaci inzercí nebo technikami genové delece. Oslabené mikroorganismy podle vynálezu mohou zahrnovat další mutace v jiných genech, například v druhém genu zde uvedeném nebo v samostatném genu důležitém pro růst mikroorganismu, například an aro mutace nebo s ohledem na Salmonelu, v genu umístěném v oblasti SP12 uváděné v WO-A-96/17951.

Oslabené mikroorganismy mohou být též použitelné jako nosné systémy pro přepravu heterologních antigenů, terapeutických proteinů nebo nukleových kyselin (DNA nebo RNA). V tomto provedení jsou oslabené mikroorganismy použitelné jako nosiče heterologního antigenů, proteinu nebo nukleové kyseliny na určité místo in vivo. Zavedením heterologního antigenů, peptidu nebo nukleové kyseliny do oslabeného mikroorganismu je možné provést známými technikami včetně použití rekombinantních konstruktů, například vektorů, které obsahují polynukleotidy, které exprimují heterologní antigen nebo terapeutický protein a obsahují též vhodné sekvence promotoru. Gen, který kóduje heterologní antigen nebo protein, může být případně včleněn do genomu mikroorganismu s endogenními promotory použitými ke kontrole exprese.

Obecněji, jak je známo odborníkům v tomto oboru, pro terapeutické použití je možné vybrat vhodné množství aktivní složky podle vynálezu, stejně jako vhodný nosič nebo excipient a způsob aplikace. Tyto faktory je možné vybrat nebo stanovit podle známých kritérií, jako například podstata/závažnost léčeného stavu, typ a/nebo zdravotní stav pacienta a podobně.

V jiném provedení mohou být produkty podle vynálezu použity pro testování identifikace potenciálního antimikrobiálního léčiva nebo pro detekci virulence. Rutinní testování jsou známá odborníkům v tomto oboru a je možné je upravit použitím produktů podle vynálezu vhodným způsobem. Například, produkty podle vynálezu mohou být cílem z hlediska použití jako potenciální léčiva se schopností inaktivovat nebo vázat cílovou částici charakterizující svoji potenciální antimikrobiální aktivitu.

Různé produkty podle vynálezu je též možné použít u veterinárních aplikací.

Následuje stručný přehled experimentálního postupu použitého pro identifikaci virulentního genu.

Příklady provedení vynálezu

K identifikaci genů u N. meningitidis, které jsou nezbytné pro septickou infekci byla použita značená (Signature-tagged) mutageneze (STM) (Hensel a kol., Science, 1995; 269; 400-403). Individuální mutanty jsou u značené mutageneze specifickými sekvenčními identifikátory, umožňujícími simultánní analýzu velkého počtu mutantů. Je však nezbytné konstruovat knihovny insertních mutantů až po studii N. meningitidis. Mutageneze byla úspěšně dokončena za použití postupu, kdy je DNA Neisseria modifikována in vitro za použití přečištěných komponentů transpozice Tn 10. Poněvadž N. meningitidis hojně využívá exogenní DNA, modifikované alely jsou pak zavedeny do N. meningitidis transformací. Mutanty pak mohu být sledovány na základě jejich schopnosti navozovat systémovou infekci.

Jako donor transpozonu pro mutagenezu in vitro byl použit vektor pSTMl 15 (Sun a kol. Nátuře Medicine, 2000: 6(11): 1269-1273). Do 96 oddělených transpozičních reakcí bylo zahrnuto 96 derivátů pSTMl 15, z nichž každý obsahoval specifickou sekvenční část. Modifikovaná genomová DNA byla opravena a vpravena do hostitele pomocí transformace. Ke zjištění, zda se inzerce Tn 10 objevuje na odlišných místech, bylo prověřeno 40 transformantů pomocí jediné transpoziční reakce analýzou Southern blot. Každá zahrnovala jedinou, odlišnou inzerci Tn 10. Pro stanovení, zda integrace Tn 10 byla stabilní během systémové infekce u mláďat krys, bylo provedeno srovnání hybridizačních vzorů šesti mutantů z krys. Byly získány identické hybridizační vzory před a po infekci.

Podmínky provedeného experimentu byly následující:

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Bakteriální kmen a růst

C311+je ET-5, serologická skupiny B izolátu N. meningitidis od pacienta s invazivní meningokokovou infekcí (Virji a kol., Mol. Microbiol., 1991; 5: 18311841). Kmen N. meningitidis byl pěstován na infuzním médiu pro mozek-srdce obsahující 5% Levinthalův doplněk. Kmeny E.coli byly namnoženy v médiu Luria Bertani. K pevnému médiu byl podle požadavků přidán kanamycin v koncentraci 75 a 50 pg/ml u N.meningitidis a respektive E.coli.

Transpozice in vitro a charakterizace inzerčního místa

K izolaci inzerčního místa pomocí markéru byl použit počátek pACYC184 replikace u pSTM115. Bylo provedeno nukleotidové sekvencování pomocí terminační metody za použití barviva (Perkin Elmer, Norwalk, Connecticut), primerů NG62 (5 '- TTGGTTAATTGGTTGTAACACTGG-3’) (SEQ ID NO. 209) nebo NG99 (5’- ATTCTCATGTTTGACAGCG-3’) (SEQ ID NO. 210). V proteinové databázi byl proveden průzkum homologie (http://www.ncbi.nim.nih.gov/) zahrnující serologické skupiny A a B N. meningitidis a genomové sekvence N. gonorhoeae (http://www.sanger.ac.uk/Projects/N_meningitidis a http://www.tigr.org a respektive http://dnal .chem.ou.edu/gono.html).

Tag amplifikace, klonování a analýza Southern blot

Hybridizace a příprava dot blot se prováděla podle popisu v Hansel a kol., supra, kromě toho, že koncové sekvence byly amplifikovány pomocí primerů NG13 (5’-ATCCTACAACCTCAAGCT-3’) (SEQ ID NO. 211) a NG14 (5’ATCCCATTCTAACCAAGC-3’) (SEQ ID NO. 212) a na membrány byly zafixovány spíše produkty PCR než plazmidová DNA. Oligonukleotidy SI (5’AAGAGATTACGCGCAGACC-3’) (SEQ ID NO. 213) a S2 (5’AATACGCAACCGCCTCTC-3’) (SEQ ID NO. 214) přiléhaly k sekvencím u pSTMl 15 vedle „signálních konců“ a byly použity k amplifikaci produktu o délce 367 párů bází u každého derivátu pSTMl 15. U Southern blot analýzy byla značena kazeta na kanamycinovou rezistanci za použití metody náhodných primerů (NEB) a byla použita jako sonda pro genomovou DNA štěpené C/al.

Zvířecí model:

Pro screening potenciálu pro značenou mutagenezi mutanty narůstaly individuálně 18 h na mikrotitračních destičkách. Bakterie se sklidily, pak rozsuspendovaly v PBS. Krysy Wistar (stáří 5 dnů) se inokulovaly intraperitoneálně 100 μΐ suspenze a pak se 48 hodin sledovaly. Ke stanovení kompetetivního indexu mutantu se množil divoký typ mutantní bakterie 18 h v pevném médiu a pak se sklidil do PBS a krysy se inokulovaly mutantem s buňkami divokého typu v poměru 1:1 v celkovém inokulu 5 χ 10⁶ CFU. Poměr mutantu (kanamycin-rezistantní) a bakterie divokého typu (citlivý na kanamycin) se stanovil přenosem vzorků replikátu do média s přídavkem nebo bez přídavku antibiotika.

Výsledky sledování homologie jsou uvedeny v tabulce 1.

Tabulka 1

SEQ. ID NO.	PROTEIN	č. přístupu	ORGANISMUS
3&4	3‘-dehydrochinát syntáza	NMB0647	N.gonorrhoeae
25 &26	Glykozytransferáza	LS12	N. gonorrhoeae
35 & 36	Polyribonucleotid- nucleotidyltransferáza	NMB0758	N. meningitidis
37 & 38	Fosforibozylformylglycin amidsyntáza	PURL	E.coli
43 & 44	Sikimátdehydrogenáza	AROE	N. meningitidis
47 & 48	Hypotetický 21,7 kD protein	NMB0673	N. meningitidis
53 & 54	Domnělý vazebný faktor buňky	NMB0345	N. meningitidis
57 & 58	Hypotetický protein	HI0633	H. influenzae
61 & 62	NaVí-Ř antiportér	NMRN0538	N. meningitidis
63 & 64	Chorizmátsyntháza	AROC	V. angulliarum
67 & 68	Parachat-indukovatelný protein B	PQ15B	E.coli
71 & 72	5 ‘ -methyltetrahydro- pteroylyltriglutamát- homocysteinmethyl- transferáza	NMB0944	N. meningitidis
79 &80	α-1,2-N-acetylglukos- amintransferáza	RFAK	N. meningitidis
83 & 84	Domnělá RNA methyláza	NMB1348	N. meningitidis
89 &90	L-laktátpermeáza	NMB0543	N. meningitidis
91 & 92	ABC transportér	NMB1240	N. meningitidis

• · · · · ······ ··· ···· * · 9 • · 9 V · « · ·

103 & 104	Pravděpodobný GTP- vazebný protein	HI0393	H.influenzae
109 & 110	Kapsle polysacchande modifikaního proteinu	LIPB	N. meningitidis
113 & 114	Hypotetický 17,8 kD protein	NMB0734	N. meningitidis
115 & 116	E. coli hypotetický protein	YIGC	S.typhimurium
119 & 120	Ribonukleáza III	NMB0686	N. meningitidis
121 & 122	AMPD protein	NMB0686	N. meningitidis
123 &124	5-methyltetra- hydroylyltriglutamát- homocysteinmethyl- transferáza	NMB0944	N. meningitidis
133 &134	Domnělá ATP dependentní RNA helikáza	NMB1422	N. meningitidis
137 & 138	Domnělá RNA methyláza	NMB1348	N. meningitidis
141 &142	Šikimátdehydrogenáza	AROE	N. meningitidis
143 &144	Domnělý vnější membránový protein	OMPU	N. meningitidis
145 & 146	TONB protein	TONB	N. meningitidis
147 & 148	Domnělý apolipoprotein N-acyltransferázy	NMB0713	N. meningitidis
149 & 150	Transponáza	NMB0991	N. meningitidis
153 &154	UTP-glukóza-1- fosfáturidylyltransferáza	NMB0638	N. meningitidis
157 & 158	ADP heptóza.LPS	NMB1527	N. meningitidis

	heptosyltransferáza II
161 & 162	Domnělá lytická mureintransglykozyláza B vážící se na membránu	NMB1279	N. meningitidis
171 &172	Domnělý faktor vážící se na membránu	NMB0345	N. meningitidis
173 &174	P-aminobenzoát- synthetáza	PABB	H.pylori J89
177 & 178	5 ‘ -methyltetrahydro- pteroylyltriglutamáthomo- cysteinmethyltransferáza	NMB0944	N. meningitidis
183 & 184	Konzervativní hypotethický protein	NMB0183	N. meningitidis
185 & 186	E. coli hypotetický protein	YIGC	S.typhi LT2

Pro zbývající sekvence zde nalezené nebyly získány žádné homologní výsledky.

K výrobě polyklonálních protilátek byly použity genové produkty, které byly testovány metodou Elisa u různých kmenů N. meningitidis ke zjištění možnosti jejich použití jako očkovacích látek. Byly použity tyto kmeny:

Neisseria meningitidis (B) Typ 1000

Neisseria meningitidis (B) Typ NGE31

Neisseria meningitidis (B) Typ NGH15

Neisseria meningitidis (B) Typ SW2 107

Neisseria meningitidis (B) Typ NHG38

Neisseria meningitidis (B) Typ NGE28

Neisseria meningitidis (B) Typ 2996

Z těchto údajů je zřejmé, kolik různých kmenů N. meningitidis je rozeznáváno těmito protilátkami.

Bakterie N. meningitidis rostla na čokoládově zbarveném Columbijském agaru s koňskou krví (Oxoid) po dobu 14 hodin při 37 °C v 5% CO2. Buňky se odstranily z agarových ploten a rozsuspendovaly ve 20 ml PBS v 50 ml zkumavce. Suspenze buněk se zahřívala 30 minut při 56 °C pro usmrcení bakterií.

μΐ vzorku bakterií usmrcených teplem N. meningitidis se navrstvilo na čokoládově zbarvený Columbii ský agar s koňskou krví a inkubovalo 18 hodin při 37 °C, 5% CO2. Tímto se usmrtily poslední buňky N. meningitidis. OD₆₂o suspenze se upravilo na 0,1 OD jednotek oproti PBS.

Elisa stanovení s buňkami N. meningitidis usmrcenými teplem

Stanovení Elisa se provádělo za použití buněk N. meningitidis usmrcených teplem podle následujícího protokolu. Elisa destičky se přes noc pokryly buňkami usmrcenými teplem (50 μΐ usmrcených bakterií v PBS v každé jamce 96 jamkové destičky a inkubovaly se při 4 °C).

Postupovalo se podle standardního protokolu Elisa vždy s 1 h inkubací při 37 °C. Použil se PBS/3% blokující roztok BSA, promývací roztok PBS/Tween 0,1%, konjugát sekundární protilátky králičí AP (Sigma) a detekční činidlo Sigma Fast P nitrofenylfosfát (Sigma). Výsledky byly detekovány při 405nm za použití příslušného readeru na mikrodetičky. Použitá séra byla získána sedm dní po první propagaci vakcinace (35.den po první vakcinaci).

Byly testovány protilátky, které se vytvořily proti genovým produktům identifikovaným jako SEQ ID NOS. 8, 102, 140, 158 a 202. V každém případě výsledky ukazují, že antiséra rozlišovala několik různých kmenů N. meningitidis B.

Screening ex vivo/in vitro

Ochrana proti meningokokovým onemocněním u pacientů byla spojována s přítomností bakteriálních protilátek proti N. meningitidis (Goldscheider a kol. J Exp. Med., 1969; 129: 1307-1326). Je též zřejmé, že je dobré použít korelaci mezi detekovatelnou baktericidní aktivitou a ochranou v modelu in vivo (viz Martin, J. Bacteriol., 2000; 83: 27-31). Vytvořené antisérum bylo proto použito k vyhodnocení baktericidní aktivity vytvořených protilátek.

Baktericidní stanovení se prováděla s pre-imunním sérem a odpovídajícím králičím antisérem vytvořeným proti případným antigenům. Bylo použito komerčně dostupné králičí sérum jako zdroj komplementu po pre-screeningu pro eliminaci usmrcení způsobeného pouze komplementem. V případě potřeby se jako pufr použil roztok Dulbecco PBS (Gibco). Kmen MC58 N. meningitidis se množil při 37 °C (5% CO2) 14 hodin před použitím ve stanovení.

200-400 CFU MC58 v 50 μΐ se inkubovalo za přítomnosti komplementu (50 μΐ) se 100 μΐ postupně ředěných roztoků séra inaktivovaného teplem. Vzorky v čase nula se na vrstvily na čokoládově zbarvený Columbijský agar s koňskou krví (Oxoid). Po 60 minutové inkubaci se stanovilo množství přežitých bakterií navrstvením na čokoládově zbarvený Columbijský agar s koňskou krví (Oxoid). Bakteriální aktivita se vyjádřila v procentech bakterií přežívajících 60 minut. Všechny vzorky se testovaly dvakrát a vrstvily se třikrát. Použily se všechny příslušné pozitivní a negativní kontroly. U každého testovaného vzorku byla baktericidní aktivita výrazně vyšší než u pre-imunního séra.

Screening in vivo

Ke stanovení ochranného účinku potenciální vakcíny se dospělé myši imunizovaly rekombinantními proteiny zde označenými jako SEQ ID NOS. 102 a 108 a ochranný účinek se stanovil bakteriální životaschopností. U každé 15. potenciální vakcíny se myš stáří šesti týdnů (šestitýdenní myš Balb/C) naočkovala (podkožně) 25pg antigenu u dvou odlišných případů v intervalu tří týdnů.

.. 4 ί* κjiuí ^r

Týden po ukončnení imunizace byla skupina vystavena homolognímu bakteriálnímu kmeni MC58. Bakterie se inokulovaly intraperitoneálně v objemu 500 μΐ v médiu infuze pro mozek a srdce/0,5 % dextran s kovem v dávce 10 CFU. Předchozí výsledky ukázaly, že kov je nezbytný k vyvolání bakteriálního onemocnění u těchto zvířat. Model byl dříve použit ke zjištění ochranného účinku očkování (Lissoio a kol., Infect. Immujn., 1995; 63: 884-890).

Kontrolní skupiny zahrnovaly zvířata očkovaná pouze adjuvans (negativní kontrola) nebo adjuvans v kombinaci s přečištěným PorA (pozitivní kontrola). PorA je vnější membránový protein exprimovaný výhradně N. meningitidis a je hlavním cílem pro bakteriální protilátky aktivované očkovací látkou membránové dutiny. Monoklonální protilátky proti PorA též pasivně chrání zvířata u modelu krysích mláďat. PorA se však významně liší v závislosti na kmeni, a protože to vede k určité ochraně při aktivaci homologním kmenem, není ideální jako potenciální očkovací látka. Přežití bylo sledováno po aktivaci. Negativní kontroly nepřežily 48 hodin. U případů očkovaných PorA přežilo po 72 hodinách 6. Případy očkovány proteiny SEQ ID NOS. 102 a 108 vykazovaly 5 a 3 přežívající případy.

7/ MM -Šéfa

Claims (20)

1.

Peptid kódovaný operonem obsahujícím kteroukoli z nukleotidových sekvencí, zde uváděných jako SEQ ID NOS. 1, 3, 5, 7, 9, 11, 13, 15, 17,19,

21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99,

101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129,

131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159,

161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189,

191,193,195, 197, 199, 201, 203, 205, 207 u N. meningitidis nebo podobné molekuly s alespoň 40% sekvenční podobností nebo shodou na úrovni peptidu nebo nukleotidu u Gram-negativní bakterie nebo jejího funkčního fragmentu pro terapeutické nebo diagnostické použití.

2. Peptid podle nároku 1, kde sekvenční podobnost nebo identita je alespoň 60%.

3. Peptid podle nároku 1 nebo nároku 2, kde sekvenční podobnost nebo identita je alespoň 90%.

4. Peptid podle nároku 1, obsahující aminokyselinovou sekvenci zde uváděnou jako SEQ ID NOS. 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66_? 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108,

110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138,

140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168,

170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198,

200, 202, 204, 206 a 208.

·· ·· • é · 99 9999 9 9 9 · 9 9 99 • · « 9 9 9 9 9 « » 9 9 ···· · · · · 99 999 99 99

5. Polynukleotid kódující peptid podle kteréhokoli z předcházejících nároků pro terapeutické nebo diagnostické použití.

6. Hostitelský organismus transformovaný k expresi peptidu podle kteréhokoli z nároků 1 až 4.

7. Oslabený mikroorganismus obsahující mutaci, která zabraňuje expresi kterékoli nukleotidové sekvence podle nároku 1.

8. Mikroorganimus podle nároku 7, kde mutace je inzerční inaktivací nebo genovou delecí.

9. Mikroorganimus podle nároku 7 nebo 8, kdy je mikroorganismem Neisseria meningitidis.

10. Mikroorganimus podle kteréhokoli z nároků 7 nebo 8 obsahující druhou mutaci v druhé nukleotidové sekvenci.

11. Mikroorganimus podle kteréhokoli z nároků 7 nebo 8 pro terapeutické a diagnostické použití.

12. Mikroorganimus podle nároků 7 až 11 obsahující heterologní antigen, terapeutický peptid nebo nukleovou kyselinu.

13. Očkovací látka obsahující peptid podle kteréhokoli z nároků 1 až 4 nebo prostředek pro její expresi.

14. Očkovací látka obsahující mikroorganismus podle kteréhokoli z nároků 7 až

12.

9· ···· ·· ·» > ·« <

··*· ····

15. Protilátka vytvořená proti peptidu podle kteréhokoli z nároků 1 až 4.

16. Použití produktu podle kteréhokoli z nároků 1 až 12 pro výrobu léčiva pro použití při léčbě nebo prevenci stavů spojených s infekcí Neisseria nebo Gramnegativní bakterií.

17. Použití podle nároku 16, kde se jedná o stav při meningitidě.

18. Použití podle nároku 16 nebo nároku 17 pro veterinární léčbu.

19. Použití produktu podle kteréhokoli z nároků 1 až 12 pro screening identifikace antimikrobiálního léčiva.

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SEZNAM SEKVENCÍ \ l

• <110> Microscience Limited <i20> Virulentní geny, proteiny a jejich použití <130> REP06436WO <i40> není ještě známo <141> 2001-05-08 <150> 0011108.8 <151> 2000-05-08 <160> 214 <170> Patentln Ver. 2.1 <210> 1 <211> 1674 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1674) <400> 1

atg Met 1 ctg acc tat acc ccg ccc gat gcc cgc ccg ccc gcc aaa acc cac 48 Leu Thr Tyr Thr 5 Pro Pro Asp Ala Arg 10 Pro Pro Ala Lys Thr 15 His gaa aag ccg tgg ctg ttg ctg ttg atg gcg ttt gcc tgg ttg tgg ccc 96 Glu Lys Pro Trp Leu Leu Leu Leu Met Ala Phe Ala Trp Leu Trp Pro 20 25 30 ggc gtg ttt tcc cac gat ttg tgg aat cct gac gaa cct gcc gtc tat 144 Gly Val Phe Ser His Asp Leu Trp Asn Pro Asp Glu Pro Ala Val Tyr 35 40 45 acc gcc gtc gaa gca ctg gca ggc age ccc acc cct ttg gtt gcc cat 192 Thr Ala Val Glu Ala Leu Ala Gly Ser Pro Thr Pro Leu Val Ala His 50 55 60 ctg ttc ggt caa atc gat ttc ggc ata ccg .ccc gtg tat ctt tgg gtt 240 Leu Phe Gly Gin Ile Asp Phe Gly Ile Pro¹ fpro Val Tyr Leu Trp Val 65 70 75 80

WO 01/85772 PCT/GBO1/02003 gcc gcc gcg ttc. aaa cat ttg ctg tcg ccg tgg get gcc gac ccg tat 288 Alat Ala Ala Phe Lys His Leu Leu Ser Pro Trp Ala Ala Asp Pro Tyr 85 90 95 gat gcc gca cgc ttt gcc ggc gtg ttt ttc gcc gtt gtc gga ctg act 336 Asp Ala Ala Arg Phe Ala Gly val Phe Phe Ala Val Val Gly Leu Thr 100 105 110 tcc tgc ggc ttt. gcc ggt ttc aac ttt ttg ggc aga cac cac ggg cgc 384 Ser Cyš Gly Phe Ala Gly Phe Asn Phe Leu Gly Arg His His Gly Arg 115 120 125 agc gtc gtc ctg att ctc atc ggc tgt atc ggg ctg att ccg acc gta 432 Ser Val Val Leu Ile Leu Ile Gly Cys Ile Gly Leu Ile Pro Thr Val 130 135 140 cac ttt ctc aac ccc get gcc gcc gcc ttt gcc gcc gcc gga ctg gtg 480 His Phe Leu Asn Pro Ala Ala Ala Ala Phe Ala Ala Ala Gly Leu Val 145 150 155 160 ctg cac ggt tat tet ttg get cgc cgg cgc gtg att gcc gcc tet ttt 528 Leu His Gly Tyr Ser Leu Ala Arg Arg Arg Val Ile Ala Ala Ser Phe 165 170 175 ctg ctc ggt ácg ggt tgg acg ctg atg tcg ttg gca gca get tat ccg 576 Leu Leu Gly Thr Gly Trp Thr Leu Met Ser Leu Ala Ala Ala Tyr Pro 180 185 190 gcg gca ttt gcc ctg átg ctg ccc ctg ccc gtg ctg atg ttt ttc cgt 624 Ala Ala Phe Ala Leu Met Leu Pro Leu Pro Val Leu Met Phe Phe Arg 195 200 205 ccg tgg caa agc agg cgt ttg atg ttg acg gca gtc gcc tcg ctt gcc 672 Pro Trp Gin Ser Arg Arg Leu Met Leu Thr Ala Val Ala Ser Leu Ala

210 215 220

ttt gcc ctg ccg ctt atg acc gtt tac ccg ctg ctc ttg gca aaa acg Ala Lys Thr 240 Phe Ala Leu 225 Pro Leu Met 230 Thr Val Tyr Pro Leu Leu 235 Leu cag ccc gcg ctg ttc gcg caa tgg ctc gac gat cac gtt ttc ggt acg Gin Pro Ala Leu Phe Ala Gin Trp Leu Asp Asp His Val Phe Gly Thr 245 250 255 ttc ggc ggc gtg cgg cac att cag acg gca ttc agt ttg ttt tac tat Phe Gly Gly Val Arg His Zle Gin Thr Ala Phe Ser Leu Phe Tyr Tyr 260 265 270

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ctg aaa aac ctg ctt tgg ttt gca Trp Phe Ala 280 ttg cct gcg ctg ccg ctg gcg gtt 864 Leu Lys Asn Leu Leu 275 Leu Pro Ala Leu Pro 285 Leu Ala Val tgg acg gtt tgc cgc acg cgc ctg ttt tcg acc gac tgg ggg att ttg 912 Trp Thr Val Cys Arg Thr Arg Leu Phe Ser Thr Asp Trp Gly Ile Leu 290 295 300 ggc gtc gtc tgg atg ctt gcc gtt ttg gtg ctg ctt gcc gtc aat ccg 960 Gly Val Val Trp Met Leu Ala Val Leu Val Leu Leu Ala Val Asn Pro 305 310 315 320 cag cgt ttt cag gat aac ctc gtc tgg ctg ctt ccg ccg ctt gcc ctg 1008 Gin Arg Phe Gin Asp Asn Leu Val Trp Leu Leu Pro Pro Leu Ala Leu 325 330 335 ttc ggc gcg gcg caa ctg gac age ctg aga cgc ggc gcg gcg gcg ttt 1056 Phe Gly Ala Ala Gin Leu Asp Ser Leu Arg Arg Gly-Ala Ala Ala Phe 340 345 350 gtc aac tgg ttc ggc att atg gcg ttc gga ctg ttt gcc gtg ttc ctg 1104 Val Asn Trp Phe Gly Ile Met Ala Phe Gly Leu Phe Ala Val Phe Leu 355 360 365 tgg acg ggc ttt ttc gcc atg aat tac ggc tgg ccc gcc aag ctt gcc 1152 Trp Thr Gly Phe Phe Ala Met Asn Tyr Gly Trp Pro Ala Lys Leu Ala 370 375 380 gaa cgc gcc gcc tat ttc age ccg tat tat gtt cct gat atc gat ccc 1200 Glu Arg Ala Ala Tyr Phe Ser Pro Tyr Tyr Val Pro Asp Ile Asp Pro 385 390 395 400 att ccg atg gcg gtt gcc gta ctg ttc aca ccc ttg tgg ctg tgg gcg 1248 Xle Pro Met Ala Val Ala Val Leu Phe Thr Pro Leu Trp Leu Trp Ala 405 410 415 att acc cgc aaa aac ata cgc ggc agg cag gcg gtt acc aac tgg gcg 1296 Ile Thr Arg Lys Asn Ile Arg Gly Arg Gin Ala Val Thr Asn Trp Ala 420 425 430 gca ggc gtt acc ctg acc tgg gct ttg ctg atg acg ctg ttc ctg ccg 1344 Ala Gly Val Thr Leu Thr Trp Ala Leu Leu Met Thr Leu Phe Leu Pro 435 440 445 tgg ctg gac gcg gcg aaa age cac gcg ccc gtc gtc cgg agt atg gag 1392 Trp Leu Asp Ala Ala Lys Ser His Ala Pro Val Val Arg Ser Met Glu 450 455 460

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gca tcg ctt tcc ccg gaa tta aaa cgg gag ctt tea gac ggc atc gag Ala Sec Leu 465 Sec Pco Glu 470 Leu Lys Arg Glu Leu 475 Ser Asp Gly Ile Glu 480 tgt atc gac ata ggc ggc ggc gac cta cac acg cgg att gtt tgg acg Cys Xle Asp Ile Gly Gly Glý Asp Leu His Thr Arg Ile Val Trp Thr 485 490 495 cag tac ggc aca ttg ccg cac cgc gtc ggc gat gta caa tgc cgc tac Gin Tyc Gly The Leu Pco His Arg Val Gly Asp Val Gin Cys Arg Tyr 500 505 510 cgc atc gtc cgc ttg ccc caa aac gcg gat gcg ccg caa ggc tgg cag Acg Ile Val Acg Leu Pco Gin Asn Ala Asp Ala Pro Gin Gly Trp Gin 515 520 525 acg gtc tgg cag ggt gcg cgc ccg cgc aac aaa gac agt aag ttc gca The Val Tep Gin Gly Ala Arg Pro Acg Asn Lys ASp Ser Lys Phe Ala 530 535 540 ctg ata cgg aaa acc ggg gaa aat ata tta aaa aca aca gat Leu Ť1 Λ 7\ *«« V T.lřO w Cl »» Asn Ti _A T.ite ·“ jr mVi mk T\

545 550 555

1440

1488

1536

1584

1632

1674 <210> 2 <211> 558 <212> PRT <213> Neisseria meningitidis <400> 2

Met 1 Leu Thr Tyr Thr 5 Pro Pro Asp Ala Arg 10 Pro Pro Ala Lys Thr 15 His Glu Lys Pro Trp 20 Leu Leu Leu Leu Met 25 Ala Phe Ala Trp Leu 30 Trp Pro Gly Val Phe 35 Ser His Asp Leu Trp 40 Asn Pro Asp Glu Pro 45 Ala Val Tyr Thr Ala 50 Val Glu Ala Leu Ala 55 Gly Ser Pro Thr Pro 60 Leu Val Ala His Leu 65 Phe Gly Gin Ile Asp 70 Phe Gly Ile Pro Pro 75 Val Tyr Leu Trp Val 80 Ala Ala Ala Phe Lys 85 His Leu Leu Ser Pro 90 Trp Ala Ala Asp Pro 95 Tyr

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Asp Ala Ala Arg 100 Phe Ala Gly Val Phe 105 Phe Ala Val Val Gly 110 Leu Thr Ser Cys Gly 115 Phe Ala Gly Phe Asn 120 Phe Leu Gly Arg His 125 His Gly Arg Ser Val 130 Val Leu Ile Leu Ile 135 Gly Cys Ile Gly Leu 140 Ilfe Pro Thr Val His 145 Phe Leu Asn Pro Ala 150 Ala Ala Ala Phe Ala 155 Ala Ala Gly Leu Val 160 Leu His Gly Tyr Ser 165 Leu Ala Arg Arg Arg 170 Val Ile Ala Ala Ser 175 Phe Leu Leu Gly Thr ISO Gly Trp Thr Leu Met 185 Ser Leu Ala Ala Ala 190 Tyr Pro Ala Ala Phe 195 Ala Leu Met Leu Pro 200 Leu Pro Val Leu Met 205 Phe Phe Arg Pro Trp 210 Gin Ser Arg Arg Leu 215 Met Leu Thr Ala Val 220 Ala Ser Leu Ala Phe 225 Ala Leu Pro Leu Met 230 Thr Val Tyr Pro Leu 235 Leu Leu Ala Lys Thr 240 Gin Pro Ala Leu Phe 245 Ala Gin Trp Leu Asp 250 Asp His Val Phe Gly 255 Thr Phe Gly Gly Val 260 Arg His Ile Gin Thr 265 Ala Phe Ser Leu Phe 270 Tyr Tyr Leu Lys Asn 275 Leu Leu Trp Phe Ala 280 Leu Pro Ala Leu Pro 285 Leu Ala Val Trp Thr 290 Val Cys Arg Thr Arg 295 Leu Phe Ser Thr Asp 300 Trp Gly Ile Leu Gly 305 Val Val Trp Met Leu 310 Ala Val Leu Val Leu 315 Leu Ala Val Asn Pro 320 Gin Arg Phe Gin Asp 325 Asn Leu Val Trp Leu 330 Leu Pro Pro Leu Ala 335 Leu Phe Gly Ala Ala Gin Leu Asp Ser Leu Arg Arg Gly Ala Ala Ala Phe

340 345 350

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Val Asn Trp Phe Gly Ile Met Ala Phe Gly Leu Phe Ala Val Phe Leu 355 360 365 Trp Thr Gly Phe Phe Ala Met Asn Tyr Gly Trp Pro Ala Lys Leu Ala 370 375 380 Glu Arg Ala Ala Tyr Phe Ser Pro Tyr Tyr Val Pro Asp Ile Asp Pro 385 390 395 400 Ile Pro Met Ala Val Ala Val Leu Phe Thr Pro Leu Trp Leu Trp Ala 405 410 415 Ile Thr Arg Lys Asn Ile Arg Gly Arg Gin Ala Val Thr Asn Trp Ala 420 425 430 Ala Gly Val Thr Leu Thr Trp Ala Leu Leu Met Thr Leu Phe Leu Pro 435 440 445 Trp Leu Asp Ala Ala Lys Ser His Ala Pro Val Val Arg Ser Met- Glu 450 455 460 Ala Ser Leu Ser Pro Glu Leu Lys Arg Glu Leu Ser Asp Gly Ile Glu 465 470 475 480 Cys Ile Asp Ile Gly Gly Gly Asp Leu His Thr Arg Ile Val Trp Thr 485 490 495 Gin Tyr Gly Thr Leu Pro His Arg Val Gly Asp Val Gin Cys Arg Tyr 500 505 510 Arg Ile Val Arg Leu Pro Gin Asn Ala Asp Ala Pro Gin Gly Trp Gin 515 520 525 Thr Val Trp Gin Gly Ala Arg Pro Arg Asn Lys Asp Ser Lys Phe Ala 530 535 540 Leu Ile Arg Lys Thr Gly Glu Asn Ile Leu Lys Thr Thr Asp 545 550 555

<210> 3 <211> 1077 <212> DNA <213> Neisseria meningitidis <220>

• · β ·

WO 01/85772 PCT/GB01/02003 <221> CDS <222> (1)..(1077) <400> 3

atg Met 1 aaa aca ctg acc gta cac acc ccg tcc cac age tac ccc atc ttt 48 Lys Thr Leu Thr Val His 5 Thr Pro Ser 10 His Ser Tyr Pro Ile 15 Phe atc ggc aac gga ctg ctg ccg cag gca gga age ctg ctc aaa ccg cat 96 Ile Gly Asn Gly Leu Leu Pro Gin Ala Gly Ser Leu Leu Lys Pro His 20 25 30 ttg ggc aaa cgc gcc gcc atc atc acc aac gaa acc gtc gcc ccg ctc 144 Leu Gly Lys Arg Ala Ala Ile Ile Thr Asn Glu Thr Val Ala Pro Leu 35 40 45 tac ctc ggc acg ctt cag acg gca ttg gat gcg gca ggc gta tcc cat 192 Tyr Leu Gly Thr Leu Gin Thr Ala Leu Asp Ala Ala Gly Val Ser His 50 55 60 ttc age atc atc ctg ccc gac ggc gag gcg cac aaa aac tgg cag acg 240 Phe Ser Ile Ile Leu Pro Asp Gly Glu Ala His Lys Asn Trp Gin Thr 65 70 75 80 ctc aac ctc atc ttt gac ggg ctg atg caa aac cgc gcc gaa cgc aaa 288 Leu Asn Leu Ile Phe Asp Gly Leu Met Gin Asn Arg Ala Glu Arg Lys 85 90 95 acc acg ctc atc gca ctg ggc ggc ggc gtg atc ggc gat atg acg ggt 336 Thr Thr Leu Ile Ala Leu Gly Gly Gly Val Ile Gly Asp Met Thr Gly 100 105 110 ttt gcg gcg get act tat caa cgc ggc gcg cca ttt gtt caa att ccc 384 Phe Ala Ála Ala Thr Tyr Gin Arg Gly Ala Pro Phe Val Gin Ile Pro 115 120 125 act act tta tta agt cag gtc gat tcc tcc gtc ggc ggc aaa acc gcc 432 Thr Thr Leu Leu Ser Gin Val Asp Ser Ser Val Gly Gly Lys Thr Ala 130 135 140 atc aac cac ccg ctc ggc aaa aat atg atc ggc gcg ttc tac cag ccg 480 Ile Asn His Pro Leu Gly Lys Asn Met Ile Gly Ala Phe Tyr Gin Pro 145 150 155 160 cag gcg gtg ctt gcc gac ttg gac acg ctg cac acc ctg ccc gcc cgc 528 Gin Ala Val Leu Ala Asp Leu Asp Thr Leu His Thr Leu Pro Ala Arg 165 170 175

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gaa ctg tcc gcc ggt atg gcg gaa gtc atc aaa tac ggc acg ctc ggc Leu Gly 576 Glu Leu Ser Ala Gly 180 Met Ala Glu Val 185 Ile Lys Tyr Gly Thr 190 gac att agt ttt ttt gaa tgg ctg gaa cag cat atg ccc gaa ctg atg 624 Asp Ile Ser Phe Phe GlU Trp Leu Glu Gin His Met Pro Glu Leu Met 195 200 205 gca ctc gaa cgc gcg ccc ctc atc caa gcc gtg tac cgc tgt tgc caa 672 Ala Leu Glu Arg Ala Pro Leu Ile Gin Ala Val Tyr Arg Cys Cys Gin 210 215 220 atg aag gca gac atc gtc gcc caa gac gaa acc gaa cag ggc ata cgc 720 Met Lys Ala Asp Ile Val Ala Gin Asp GlU Thr Glu Gin Gly Ile Arg 225 230 235 240 gca tgg ctc aac ctc gga cac acc ttc gga cac gcc att gaa acc gag 768 Ala Trp Leu Asn Leu Gly His Thr Phe Gly His Ala Ile Glu Thr Glu 245 250 255 atg ggt tac ggt act tgg ctg cac ggc gag gcc gtc gcc gcc ggc tgc 816 /•T «« /“«Ί \7.X Jř mV «a x xx_r LGu Kis Zil «» Glu Ala Va 1 ala ala 260 265 270 gtg ttg gcg gcg cgt ttg tcc gaa caa ctg ggc aaa atc tcc gcc gca 864 Val Leu Ala Ala Arg Leu Ser Glu Gin Leu Gly Lys Ile Ser Ala Ala 275 280 285 gat acc gcg cgg ctc gcc gcc ctg ctc gaa gčc gcc gga ctg ccg tcc 912 Asp Thr Ala Arg Leu Ala Ala Leu Leu GlU Ala Ala Gly Leu Pro Ser 290 295 300 gcg cca ccc gtg ttt gcc ttt gaa aaa tgg ctg gaa cac atg agc cac 960 Ala Pro Pro Val Phe Ala Phe GlU Lys Trp Leu Glu His Met šer His 305 310 315 320 gat aaa aaa gtc agc ggc ggc atc atg cgc ttt atc ggt ctg aac cgg 1008 Asp Lys Lys Val Ser Gly Gly Ile Met Arg Phe Ile Gly Leu Asn Arg 325 330 335 ctg ggc gaa gcc aac atc acc gaa att acc gac acg gac atc ctc cgc 1056 Leu Gly GlU Ala Asn Ile Thr Glu Ile Thr Asp Thr Asp Ile Leu Arg

340 345 350 cgc acc ctg caa ccg tat ctc Arg Thr Leu Gin Pro Tyr Leu

355

1077 «·

WO 01/85772 PCT/GBOV02003 <210> 4 <211> 359 <212> PRT <213> Neisseria meningitidis <400> 4

Met 1 Lys Thr Leu Thr 5 Val His Thr Pro Ser His Ser Tyr Pro Ile Phe 10 15 Ile Gly Asn Gly 20 Leu Leu Pro Gin Ala Gly Ser 25 Leu Leu Lys Pro His 30 Leu Gly Lys Arg 35 Ala Ala Ile Ile Thr Asn Glu 40 Thr Val Ala Pro Leu 45 Tyr Leu Gly Thr 50 Leu Gin Thr Ala Leu Asp Ala 55 Ala Gly Val Ser His 60 Phe 65 Ser Ile Ile Leu Pro . 70 Asp Gly Glu Ala His 75 Lys Asn Trp Gin Thr 80 Leu Asn Leu Ile Phe 85 Asp Gly Leu Met Gin Asn 90 Arg Ala Glu Arg Lys 95 Thr Thr Leu Ile 100 Ala Leu Gly Gly Gly Val Ile 105 Gly Asp Met Thr Gly 110 Phe Ala Ala Ala 115 Thr Tyr Gin Arg Gly Ala Pro 120 Phe Val Gin Ile Pro 125 Thr Thr Leu Leu 130 Ser Gin Val Asp Ser Ser Val 135 Gly Gly' Lys Thr Ala 140 Ile 145 Asn His Pro Leu Gly 150 Lys Asn Met Ile Gly 155 Ala Phe Tyr Gin Pro 160 Gin Ala Val Leu Ala 165 Asp Leu Asp Thr Leu His 170 Thr Leu Pro Ala Arg 175 Glu Leu Ser Ala 180 Gly Met Ala Glu Val Ile Lys 185 Tyr Gly Thr Leu Gly 190 Asp Ile Ser Phe 195 Phe Glu Trp Leu Glu Gin His 200 Met Pro Glu Leu Met 205 Ala Leu Glu Arg Ala Pro Leu Ile Gin Ala Val Tyr Arg Cys Cys Gin

210 215 220

U^íp.'

WO 01/85772

PCT/GB01/02003

Met Lys Ala Asp Ile Val Ala Gin Asp Glu Thr Glu Gin Gly Ile Arg 225 230 235 240 Ala Trp Leu Asn Leu Gly His Thr Phe Gly His Ala Ile Glu Thr Glu 245 250 255 Met Gly Tyr Gly Thr Trp Leu His Gly Glu Ala Val Ala Ala Gly Cys 260 265 270 Val Leu Ala Ala Arg Leu Ser Glu Gin Leu Gly Lys Ile Ser Ala Ala 275 280 285 Asp Thr Ala Arg Leu Ala Ala Leu Leu Glu Ala Ala Gly Leu Pro Ser 290 295 300 Ala Pro Pro Val Phe Ala Phe Glu Lys Trp Leu Glu His Met Ser His 305 310 315 320 Asp Lys Lys Val Ser Gly Gly Ile Met Arg Phe Ile Gly Leu Asn Arg 325 330 335 Leu Gly Glu Ala Asn Ile Thr Glu Ile Thr Asp Thr Asp Ile Leu Arg 340 345 350 Arg Thr Leu Gin Pro Tyr Leu

355 <210> 5 <211> 1053 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1).. (1053) <400> 5 atg aca cac cat tac CCC aca gac gat att aag att aaa gaa gtt aaa 48 Met Thr His His Tyr Pro Thr Asp Asp Ile Lys Ile Lys Glu Val Lys 1 5 10 15 gag ttg ttg ccg ccg att gcc cat ctt tac gag ctg ccg att tcc aaa 96 Glu Leu Leu Pro Pro Ile Ala His Leu Tyr Glu Leu Pro Ile Ser Lys 20 25 30

·· ·· ·· · ·· ···· • · · · ····. · · · • · · · · · · · * · · · · · * · · · • · ·· · β β β β ········ ·· ··· ·· ··

WO 01/85772 PCT/GBO1/02003 gag gct tcg ggc ttg gtt cac cgc acc cgt cag gaa att tcc gat ttg 144 Glu Ala Ser Gly Leu Val His Arg Thr Arg Gin Glu Xle Ser Asp Leu 35 40 45 gtt cac ggc agg gac aag cgg ctg ttg gtt att atc ggg ccg tgt tcg 192 Val His Gly Arg Asp Lys Arg Leu Leu Val Xle Xle Gly Pro Cys Ser 50 55 60 att cac gat ccg aaa gcg gcg ttg gaa tat gcg gag cgt ttg ttg aaa 240 Xle His Asp Pro Lys Ala Ala Leu Glu Tyr Ala Glu Arg Leu Leu Lys

65 70 75 S0

ctc Leu cgc aag cag tat gaa aac gag ctt ttg att gtg atg cgc gtt tat 288 Arg Lys Gin Tyr 85 Glu Asn Glu Leu Leu 90 Ile Val Met Arg Val 95 Tyr ttc gag aag ccg agg acg acg gtg ggt tgg aaa ggt ttg att aac gac 336 Phe Glu Lys Pro Arg Thr Thr Val Gly Trp Lys Gly Leu Ile Asn Asp 100 105 110 ccg cat ttg gac ggt acg ttt gac atc aat ttc ggt ttg cgt cag gca 384 Pro His Leu Asp Gly Thr Phe Asp Ile Asn Phe Gly Leu Arg Gin Ala 115 120 125 cgc agc ctg ttg ttg tcg ctg aac aat atg ggt atg cct gcc tet acc 432 Arg Ser Leu Leu Leu Ser Leu Asn Asn Met Gly Met Pro Ala Ser Thr 130 135 140 gag ttt ttg gat atg att acg ccg caa tat tat gcg gac ttg att tet 480 Glu Phe Leu Asp Met Ile Thr Pro Gin Tyr Tyr Ala Asp. Leu Ile Ser 145 150 155 160 tgg ggg gca atc ggc gcg cgg acg acc gaa agc caa gtt cac cgc gaa 528 Trp Gly Ala Ile Gly Ala Arg Thr Thr Glu Ser Gin Val His Arg Glu 165 170 175 ttg gca agc ggg ctg tcc tgc ccc gtc ggc ttt aaa aac ggt acg gat 576 Leu Ala Ser Gly Leu Ser Cys Pro Val Gly Phe Lys Asn Gly Thr Asp 180 185 190 ggc aat ttg aag att gcc atc gac gca atc ggt gcg gcg agc cat tcg 624 Gly Asn Leu Lys Ile Ala Xle Asp Ala Ile Gly Ala Ala Ser His Ser 195 200 205 cat cat ttc ctg tet gta acc aag gcc ggg cat tcc gcc att gtc cat 672 His His Phe Leu Ser Val Thr Lys Ala Gly His Ser Ala Ile Val His 210 215 220

7, 7’ _ . -feíi ' -.řiAí.W'·- · . ?. ' .¹¾¼ • · frfrfrfr

WO 01/85772 PCT/GB01/02003 frfr ·· • · · ♦ • « » · β fr •frfrfr····

acc ggc ggc aat cct gac Asp 230 tgt cat gtc att ttg ege ggc gga aaa gag 720 Thr 225 Gly Gly Asn Pro Cys His Val Ile Leu 235 Arg Gly Gly Lys Glu 240 ccg aat tat gat gcg gga cac gtc age gag gcg gcg gaa caa ctg cgt ' 768 Pro Asn Tyr Asp Ala Gly His Val Ser Glu Ala Ala Glu Gin Leu Arg 245 250 255 gcg gca ggc gta acc gac aag ctg atg att gat tgc age cac gcc aac 816 Ala Ala Gly Val Thr Asp Lys Leu Met Ile Asp Cys Ser His Ala Asn 260 265 270 age ege aag gat tac acc ega cag atg gaa gtg gcg caa gac att gcc 864 ser Arg Lys Asp Tyr Thr Arg Gin Met Glu Val Ala Gin Asp Ile Ala 275 280 285 gcc caa ttg gaa cag gac ggc ggc aat att atg ggc gtg atg gtg gaa 912 Ala Gin Leu Glu Gin Asp Gly Gly Asn Ile Met Gly Val Met Val Glu 290 295 300 age cat ttg gtc gaa ggc agg cag gac aag ccg gaa gtg tac ggc aaa 960 Ser His Leu Val Glu Gly Arg Gin Asp Lys Pro Glu Val Tyr Λ1 v —- j T»V« -~j— 305 310 315 320 age att acc gat gcg tgt att ggt tgg gac gcg act gaa gaa ctg ttg 1008 Ser Ile Thr Asp Ala Cys Ile Gly Trp Asp Ala Thr Glu Glu Leu Leu 325 330 335 gca ttg ttg gca ggt gca aac aaa aaa cgt atg gcg ege gcc ggt 1053 Ala Leu Leu Ala Gly Ala Asn Lys Lys Arg Met Ala Arg Ala Gly

340 345 350 <210> 6 <211> 351 <212> PRT <213> Neisseria meningitidis <400> 6

Met Thr His His Tyr Pro ,Thr Asp Asp Ile Lys Ile Lys Glu Val Lys 1 5 10 15 Glu Leu Leu Pro Pro Ile Ala His Leu Tyr Glu Leu Pro Ile Ser Lys 20 25 30 Glu Ala Ser Gly Leu Val His Arg Thr Arg Gin Glu Ile Ser Asp Leu

35 40 45

WO 01/85772 ·· ··

4 β •••fc···· • · ·· • · 4 * · · ··· «· ··· » · · fcfc · • · · · ·· ··

PCT/GB01/02003

Val His 50 Gly Arg Asp Lys Arg 55 Leu Leu Val Ile Ile 60 Gly Pro Cys Ser Ile 65 His Asp Pro Lys Ala 70 Ala Leu Glu Tyr Ala 75 Glu Arg Leu Leu Lys 80 Leu Arg Lys Gin Tyr 85 Glu Asn Glu Leu Leu 90 Ile Val Met Arg Val 95 Tyr Phe Glu Lys Pro 100 Arg Thr Thr Val Gly 105 Trp Lys Gly Leu Ile 110 Asn Asp Pro His Leu 115 Asp Gly Thr Phe Asp 120 Ile Asn Phe Gly Leu 125 Arg Gin Ala Arg Ser 130 Leu Leu Leu Ser Leu 135 Asn Asn Met Gly Met 140 Pro Ala Ser Thr Glu 145 Phe Leu ASp Met Ile 150 Thr Pro Gin Tyr Tyr 155 Ala Asp Leu Ile ser 160 Trp Gly Ala Ile Gly 165 Ala Arg Thr Thr Glu 170 Ser Glň Val His Arg 175 Glu Leu Ala Ser Gly 180 Leu Ser Cys Pro Val 185 Gly Phe Lys Asn Gly 190 Thr Asp Gly Asn Leu 195 Lys Ile Ala Ile Asp 200 Ala Ile Gly Ala Ala 205 Ser His Ser His His 210 Phe Leu Ser Val Thr 215 Lys Ala Gly His Ser 220 Ala Ile Val His Thr 225 Gly Gly Asn Pro Asp 230 Cys His Val Ile Leu 235 Arg Gly Gly Lys Glu 240 Pro Asn Tyr Asp Ala 245 Gly His Val Ser Glu 250 Ala Ala Glu Gin Leu 255 Arg Ala Ala Gly Val 260 Thr Asp Lys Leu Met 265 Ile Asp Cys Ser His 270 Ala Asn Ser Arg Lys 275 Asp Tyr Thr Arg Gin 280 Met Glu Val Ala Gin 285 Asp Ile Ala Ala Gin 290 Leu Glu Gin Asp Gly 295 Gly Asn Ile Met Gly 300 Val Met Val Glu

. Λ»·

WO 01/85772

PCT/GBO1/02003

Ser His Leu Val Glu Gly Arg Gin Asp Lys Pro Glu Val Tyr Gly Lys 305 310 315 320 Ser Ile Thr Asp Ala Cys Ile Gly Trp Asp Ala Thr Glu Glu Leu Leu 325 330 335 Ala Leu Leu Ala Gly Ala Asn Lys Lys Arg Met Ala Arg Ala Gly 340 345 350

<210> 7 <211> 1686 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1686) <400> 7

atg tta caa tcc gaa aat tcg aaa aat 1 T,—«. UdA Z··, — VX11 Ser Glu 5 Asn Ser Lys Asn gct tta cca atc atg att tet acc aac Ala Leu Pro Ile 20 Met Ile Ser Thr Asn 25 gcg aag ccg ctg ttt gaa aac gta tcc Ala Lys Pro 35 Leu Phe Glu Asn Val 40 Ser cgc tac ggt ttg atc ggc gcg aac ggc Arg Tyr 50 Gly Leu Ile Gly Ala 55 Asn Gly aaa atc ctc ggc ggc gat ttg gaa cag Lys 65 Ile Leu Gly Gly Asp 70 Leu Glu Gin gaa aac ggc gtg cgt ttg ggt aaa ttg Glu Asn Gly Val Arg 85 Leu Gly Lys Leu gaa gat atg cgc gtg ctg gac gtg gta Glu Asp Met Arg 100 Val Leu Asp Val Val 105

tta atc tet tgt tea ata aag 48 Leu 10 Xle ser Cys Ser Ile 15 Lys ggc atc acc atg cag ttc ggc 96 Gly Ile Thr Met Gin 30 Phe Gly gtt aaa ttc ggc gaa ggc aac 144 Val Lys Phe Gly 45 Glu Gly Asn tea ggc aaa tcc acc ttc atg 192 Ser Gly Lys 60 Ser Thr Phe Met aca gcc ggc gaa gtg gcg att 240 Thr Ala 75 Gly Glu Val Ala Ile 80 cgc caa gac cag ttt gcc tac 288 Arg 90 Gin Asp Gin Phe Ala 95 Tyr atg atg ggt cac acc gaa atg 336 Met Met Gly His Thr Glu Met

110 • f

WO 01/85772

PCT/GBO1/02003

tgg gcg gcg atg acc gaa cgt gat Glu Arg Asp 120 gcg att tac gcc aat ccc gaa Glu gcc Ala 384 Tep Ala Ala Met Thr 115 Ala Ile Tyr Ala Asn 125 Pro acc gaa gac gac tac atg aaa gcc gca gaa ctg gaa gcc aag ttc gcc 432 Thr Glu Asp Asp Tyr Met Lys Ala Ala Glu Leu Glu Ala Lys Phe Ala 130 135 140 gaa tac gac ggc tac acc gcc gaa gcg cgt gcc gcc gag ttg ttg age 480 Glu Tyr Asp Gly Tyr Thr Ala Glu Ala Arg Ala Ala Glu Leu Leu Ser 145 150 155 160 ggc gtg ggc att tcc gaa gat ttg cac aat gca acc atg gca gaa gtt 528 Gly Val Gly Zle Ser Glu Asp Leu His Asn Ala Thr Met Ala Glu Val 165 170 175 gcc ccg ggc ttc aaa ctg ege gta ttg ctg gcg caa gcc ctg ttc tcc 576 Ala Pro Gly Phe Lys Leu Arg Val Leu Leu Ala Gin Ala Leu Phe Ser 180 185 190 aag ccg gat gta ttg ctc ttg gac gaa ccg acc aat aac ttg gac att 624 Lys Pro Asp Val Leu Τ.ΑΙΪ T.er Glu D*>a Thr Asn rasu T AJCSU Asp Zlé 135 200 205 aat acc atc cgt tgg ttg gaa ggc gtg ttg aac cag tac gac tcc acg 672 Asn Thr Zle Arg Trp Leu Glu Gly Val Leu Asn Gin Tyr Asp Ser Thr 210 215 220 atg att atc atc tcg cac gac ege cac ttc ttg aac gaa gtc tgc acg 720 Met Zle Zle Zle Ser His Asp Arg His Phe Leu Asn Glu Val Cys Thr 225 230 235 240 cat atg gca gat ttg gat tac aac acc atc acc atc tat ccg ggc aac 768 His Met Ala Asp Leu Asp Tyr Asn Thr Ile Thr Ile Tyr Pro Gly Asn 245 250 255 tac gac gac tat atg ctc gcc tcc gcc caa tcg ege gaa ege gcc ctg 816 Tyr Asp Asp Tyr Met Leu Ala Ser Ala Gin Ser Arg Glu Arg Ala Leu 260 - 265 270 aaa gac aat gcc aag gcg aag gaa aaa ttg cag gaa ttg caa gaa ttt 864 Lys Asp Asn Ala Lys Ala Lys Glu Lys Leu Gin Glu Leu Gin Glu Phe 275 280 285 gtg gcg cgg ttc agt gcc aac aaa tcc aaa gcc cgt cag gca acc age 912 Val Ala Arg Phe Ser Ala Asn Lys Ser Lys Ala Arg Gin Ala Thr Ser 290 295 300

β '4 ·

WO 01/85772

PCT/GB01/02003

cgt ctg aaa cag gca gac aaa atc aaa tcg gag atg gtc gag gtc aaa 960 Arg 305 Leu Lys Gin Ala Asp 310 Lys Ile Lys Ser Glu 315 Met Val Glu Val Lys 320 ccc tet acc cgt caa aac ccg tat atc cgt ttt gaa gcc gat gaa aaa 1008 Pro Ser Thr Arg Gin Asn Pro Tyr Ile Arg Phe Glu Ala Asp Glu Lys 325 330 - 335 gcc aag ctg cac cgt cag get gtg gaa gtt gaa aaa ctg gcg aaa' ege 1056 Ala Lys Leu His Arg Gin Ala Val Glu Val Glu Lys Leu Ala Lys Arg 340 345 350 ttt gaa acc cag ttg ttt aaa aac ctg aac ttt atc ctt gaa gcg gga 1104 Phe Glu Thr Gin Leu Phe Lys Asn Leu Asn Phe Ile Leu Glu Ala Gly 355 360 365 caa ege ctc gcc atc atc ggc ccg aac ggc gcg ggc aaa tcc acc ctg 1152 Gin Arg Leu Ala Ile Ile Gly Pro Asn Gly Ala Gly Lys Ser Thr Leu 370 375 380 ctg aaa ctc ttg gcc ggc gcg tac aac ccc gaa tat tca gac ggt ctg 1200 Leu Lys Leu Leu Ala Gly Ala Tyr Asn Pro Glu Tyr Ser Asp Gly Leu 385 390 395 400 ctg ccg gac gaa ggc age atc aaa tgg gcg gaá aaa gcc age gtc ggc 1248 Leu Pro Asp Glu Gly Ser Ile Lys Trp Ala Glu Lys Ala Ser Val Gly 405 410 415 tac tat ccg caa gat cac gaa aac gac ttc gat gtc gat atg gat ttg 1296 Tyr Tyr Pro Gin Asp His Glu Asn Asp Phe Asp Val Asp Met Asp Leu 420 425 430 age gaa tgg atg ege caa tgg ggg cag gac ggc gac gac gaa caa gtc 1344 Ser Glu Trp Met Arg Gin Trp Gly Gin Asp Gly Asp Asp Glu Gin Val 435 440 445 atc ege ggc act ttg ggg cgt ttg ctc ttc ggc agt aac gat gtc gtg 1392 Ile Arg Gly Thr Leu Gly Arg Leu Leu Phe Gly Ser Asn Asp Val Val 450 -455 460 aaa aaa gtg aag gtt ctc tcc ggt ggt gaa aaa ggc cgt atg ctt tac 1440 Lys Lys, Val Lys Val Leu Ser Gly Gly Glu Lys Gly Arg Met Leu Tyr 465 470 475 480 ggc aaa ctg ttg ctg ttg aaa ccc aat gtc tta gtc atg gat gaa ccg 1488 Gly Lys Leu Leu Leu Leu Lys Pro Asn Val Leu Val Met Asp Glu Pro 485 490 495

A16

WO 01/85772

PCT/GB01/02003

acc Thr aac Asn cac atg gac atg gaa age atc gaa tcc ttg aac atg gcg ctg 1536 His Met Asp Met 500 Glu Ser Ile 505 Glu Ser Leu Asn Met Ala 510 Leu gaa aaa tac aac ggc acg ctg att ttc gtc tcc cac gac cgt cag ttc 1584 Glu Lys Tyr Asn Gly Thr Leu Ile Phe Val Ser His Asp Arg Gin Phe 515 520 525 gtt tet tcc ctg gct acc caa atc att gaa ttg gac ggc aaa ggc gga 1632 Val Ser Ser Leu Ala Thr Gin Ile Ile Glu Leu Asp Gly Lys Gly Gly 530 535 540 tat gaa cac tac ttg ggc gat tac gaa age tat ctc gag aaa aaa ggc 1680 Tyr Glu His Tyr Leu Gly Asp Tyr Glu Ser Tyr Leu GlU Lys Lys Gly 545 550 555 560

gta gca 1686

Val Ala <210> 8 <211> 562 <212> PRT <213> Neisseria meningitidis <400> 8

Met 1 Leu Gin Ser Glu 5 Asn Ser Lys Asn Leu 10 Ile Ser Cys Ser Ile 15 Lys Ala Leu Pro Ile 20 Met Ile Ser Thr Asn 25 Gly Ile Thr Met Gin 30 Phe Gly Ala Lys Pro 35 Leu Phe Glu Asn Val 40 Ser Val Lys Phe Gly 45 Glu Gly Asn Arg Tyr 50 Gly Leu Ile Gly Ala 55 Asn Gly Ser Gly Lys 60 Ser Thr Phe Met Lys 65 Ile Leu Gly Gly Asp 70 Leu Glu Gin Thr Ala 75 Gly Glu Val Ala Ile 80 Glu Asn Gly Val Arg 85 Leu Gly Lys Leu Arg 90 Gin Asp Gin Phe Ala 95 Tyr Glu Asp Met Arg 100 Val Leu Asp Val Val 105 Met Met Gly His Thr 110 Glu Met

v * • · fcfc · · t fcfcfcfc • · · · · · · · · · fc fc fcfc · fc fc

WO 01/85772 PCT/GBO1/02003 • · · · · ··· ••••••fcfc ·· ··· ·· ·

Trp Ala Ala 115 Met Thr Glu Arg Asp 120 Ala Ile Tyr Ala Asn 125 Pro Glu Ala Thr Glu 130 Asp Asp Tyr Met Lys 135 Ala Ala Glu Leu Glu 140 Ala Lys Phe Ala Glu 145 Tyr Asp Gly Tyr Thr 150 Ala Glu Ala Arg Ala 155 Ala Glu Leu Leu Ser 160 Gly Val Gly Ile Ser 165 Glu Asp Leu His Asn 170 Ala Thr Met Ala Glu 175 Val Ala Pro Gly Phe 180 Lys Leu Arg Val Leu 185 Leu Ala Gin Ala Leu 190 Phe Ser Lys Pro Asp 195 Val Leu Leu Leu Asp 200 Glu Pro Thr Asn Asn 205 Leu Asp Ile Asn Thr 210 Ile Arg Trp Leu Glu 215 Gly Val Leu Asn Gin 220 Tyr Asp Ser Thr Met 225 Ile Ile Ile Ser His 230 Asp Arg His Phe Leu 235 Asn Glu Val Cys Thr 240 His Met Ala Asp Leu 245 Asp Tyr Asn Thr Ile 250 Thr Ile Tyr Pro Gly 255 Asn Tyr Asp Asp Tyr 260 Met Leu Ala Ser Ala 265 Gin Ser Arg Glu Arg 270 Ala Leu Lys Asp Asn 275 Ala Lys Ala Lys Glu 280 Lys Leu Gin Glu Leu 285 Gin Glu Phe Val Ala 290 Arg Phe Ser Ala Asn 295 Lys Ser Lys Ala Arg 300 Gin Ala Thr Ser Arg 305 Leu Lys Gin Ala Asp 310 Lys Ile Lys Ser Glu 315 Met Val Glu Val Lys 320 Pro Ser Thr Arg Gin 325 Asn .Pro Tyr Ile Arg 330 Phe Glu Ala Asp Glu 335 Lys Ala Lys Leu His 340 Arg Gin Ala Val Glu 345 Val Glu Lys Leu Ala 350 Lys Arg Phe Glu Thr 355 Gin Leu Phe Lys Asn 360 Leu Asn Phe Ile Leu 365 Glu Ala Gly

00 • • ·«· 00 0 0 0 0 000 0 0 00 0 0 • 0 0 0 0 •00 0 00 000 0 WO 01/85772 PCT/GB01/02003 Gin Arg Leu Ala Ile Ile Gly Pro Asn Gly Ala Gly Lys Ser Thr Leu 370 375 380 Leu Lys Leu Leu Ala Gly Ala Tyr Asn Pro Glu Tyr Ser Asp Gly Leu 385 390 395 400 Leu Pro Asp Glu Gly Ser Ile Lys Trp Ala Glu Lys Ala Ser Val Gly 405 410 415 Tyr Tyr Pro Gin Asp His Glu Asn Asp Phe Asp Val Asp Met Asp Leu 420 425 430 Ser Glu Trp Met Arg Gin Trp Gly Gin Asp Gly Asp Asp Glu Gin val 435 440 445 Ile Arg Gly Thr Leu Gly Arg Leu Leu Phe Gly Ser Asn Asp Val Val 450 455 460 Lys Lys Val Lys Val Leu Ser Gly Gly Glu Lys Gly Arg Met Leu Tyr 465 470 475 480 Gly Lys Leu Leu Leu Leu Lys Pro Asn Val Leu Val Met Asp Glu Pro 485 490 495 Thr Asn His Met Asp Met Glu Ser Ile Glu Ser Leu Asn Met Ala Leu 500 505 510 Glu Lys Tyr Asn Gly Thr Leu Ile Phe Val Ser His Asp Arg Gin Phe 515 520 525 Val Ser Ser Leu Ala Thr Gin Ile Ile Glu Leu Asp Gly Lys Gly Gly 530 535 540 Tyr Glu His Tyr Leu Gly Asp Tyr Glu Ser Tyr Leu Glu Lys Lys Gly 545 550 555 560

Val Ala <210> 9 <211> 1632 <212> DNA <213> Neisseria meningitidis <220 <221> CDS

44 44 44 • · < ♦ · » ·· 4 * y • · 4 4 4 44« • · 4 4 · 4 4 · 9 4

4 4 44 4 4 444 • 444 4444 44 444 44 44 •4 444»

WO 01/85772 PCT/GB01/02003 <222> (1)..(1632) <400> 9

atg ata aaa ccg aac ctg agg ccg aag ctc ggc tet tcc gcg ctg att 48 Met 1 Ile Lys Pro Asn 5 Leu Arg Pro Lys Leu 10 Gly Ser Ser Ala Leu 15 Ile gcc ttc ctt tcc ctg tat tcc tcg ctg gta ttg aat tac gcc ttt ttt 96 Ala Phe Leu Ser Leu Tyr Ser Ser Leu Val Leu Asn Tyr Ala Phe Phe 20 25 30 gcc aaa gtt gtc gag ctt cat cct ttt aac ggc acc ggg gcg gat atc 144 Ala Lys Val Val Glu Leu His Pro Phe Asn Gly Thr Gly Ala Asp Ile 35 40 45 ttc ctc tat acg atg ccg gtg gtg ctg ttt ttt tta aat aat ttc gtt 192 Phe Leu Tyr Thr Met Pro Val Val Leu Phe Phe Leu Ser Asn Phe Val 50 55 60 ttt cac gtc att gcc ctg cct ttc gtg cat aag gta ttg att ccg ttg 240 Phe His Val Ile Ala Leu Pro Phe Val His Lys Val Leu Ile Pro Leu 65 70 75 80 ata ttg gtt atc agt gcg gcg gtg tet tac caa gaa ata ttt ttc aat 288 Ile Leu Val Ile Ser Ala Ala Val Ser Tyr Gin Glu Ile Phe Phe Asn 85 90 95 atc tat ttc aac aag tcg atg ttg aat aat gtc ttg caa act acg gct 336 Ile Tyr Phe Asn Lys Ser Met Leu Asn Asn Val Leu Gin Thr Thr Ala 100 105 110 gcc gaa age gcg cgc ctg att acg ccg ggc tat .gtg ctg tgg att gta 384 Ala Glu Ser Ala Arg Leu Ile Thr Pro Gly Tyr val Leu Trp Ile Val 115 120 125 tgt ttg ggc gta ttg ccc gcg ctg gcg tat atc gcc gtc aag gtt aaa 432 Cys Leu Gly Val Leu Pro Ala Leu Ala Tyr Ile Ala Val Lys Val Lys 130 135 140 tac cgc gtt tgg tat aag -gag ctt ttg acg cgc ctt gtg ctt gcc gcc 480 Tyr Arg Val Trp Tyr Lys Glu Leu Leu Thr Arg Leu Val Leu Ala Ala 145 150 155 160 gtt tcc ttt ttg tgc gcg ttg ggc atc gca atg ttg caa tat cag gat 528 Val Ser Phe Leu Cys Ala Leu Gly Ile Ala Met Leu Gin Tyr Gin Asp 165 170 175 tac gcc tcg ttt ttc cgc aac aat aaa tca gta acc cat ctg att gtg 576

WO 01/85772

PCT/GB01/02003

Tyr Ala Ser Phe Phe Arg Asn Asn Lys Ser Val Thr His Leu Ile Val 180 185 190 cca tet aat ttc atc ggc gcg ggc gtg tcg aaa tac aaa gat tgg aag 624 Pro Ser Asn Phe Ile Gly Ala Gly Val Ser Lys Tyr Lys Asp Trp Lys 195 200 205 cgt tcc aat att cct tat acg caa ttg gat atg gcg gtc gtg caa aac 672 Arg Ser Asn Ile Pro Tyr Thr Gin Leu Asp Met Ala Val Val Gin Asn 210 215 220 cgc ccg gcc ggc agc ctg cgc cgt ttc gtg gtg ctg gtc gtg ggc gag 720 Arg Pro Ala Gly Ser Leu Arg Arg Phe Val Val Leu Val Val Gly Glu 225 230 235 240 acc acg cgt gcc gcc aac tgg ggt ttg aac ggt tac agc cgc caa act 768 Thr Thr Arg Λ T — AIa Asn Trp Gly Leu Asn Gly Tyr ser Arg Gin Thr 245 250 255 acg ccg ctg ctt gcc gcg cgc ggc gat gaa att gtc aat ttc ccg cag 816 Thr Pro Leu Leu Ala Ala Arg Gly Asp Glu Ile Val Asn Phe Pro Gin 260 265 270 gtc aga agc tgc ggt aca tcg acc gcg cac tcc ctg ccg tgt atg ttc 864 Val Arg Ser Cys Gly Thr Ser Thr Ala His Ser Leu Pro Cys Met Phe 275 280 285 tca acc ttc gac cgc acg gat tat gac gaa atc aaa gcc gaa cac caa 912 Ser Thr Phe Asp Arg Thr Asp Tyr Asp Glu Ile Lys Ala Glu His Gin 290 295 300 gac aac ctg ctg gac atc gtg cag cgc gcc ggc gtg gaa gtt acc tgg 960 Asp Asn Leu Leu Asp Ile Val Gin Arg Ala Gly Val Glu Val Thr Trp 305 310 315 320 ttg gaa aac gat tcc ggc tgc aag ggc gtg tgc ggc aaa gtg ccg aat 1008 Leu Glu Asn Asp Ser Gly Cys Lys Gly Val Cys Gly Lys Val Pro Asn 325 330 335 acc gac gtt acc tcg ctc aac ctg ccc gaa tac tgc cgc aac ggc gag 1056 Thr Asp Val Thr Ser Leu Asn Leu Pro Glu Tyr Cys Arg Asn Gly Glu 340 345 350 tgc ctc gac aat atc ctg ctg acc aag ttc gac gaa gtc ctc aac aaa 1104 Cys Leu Asp Asn Ile Leu Leu Thr Lys Phe Asp Glu Val Leu Asn Lys 355 360 365 aac gat aaa gac gcg gtt tta atc ctg cat acc atc ggc agc cac ggg 1152

-MÁ:

I

WO 01/85772 PCT/GB01/02003

Asn Asp 370 Lys Asp Ala Val Leu 375 Ile Leu His Thr Ile 380 Gly Ser His Gly ccg acg tat tac gaa cgc tat acc gaa gcc gaa cgc aaa ttc acg ccg 1200 Pro 385 Thr Tyr Tyr Glu Arg 390 Tyr Thr Glu Ala Glu 395 Arg Lys Phe Thr Pro 400 acc tgc gac acc aac gaa atc aac aaa tgc acc cgc gcc acg ctg gtc 1248 Thr Cys Asp Thr Asn 405 Glu Ile Asn Lys Cys 410 Thr Arg Ala Thr Leu 415 Val aac act tac gac aat acg gtt ttg tat gtg gac cag ttt atc gac aag 1296 Asn Thr Tyr Asp 420 Asn Thr Val Leu Tyr 425 val Asp Gin Phe Ile 430 Asp Lys gtt atc cgc aaa ctt gaa aac cgc gac gat ttg gaa age gtg gtg cat 1344 Val Ile Arg 435 Lys Leu Glu Asn Arg 440 Asp Asp Leu Glu Ser 445 Val Val His tat gtt tcc gac cac ggc gaa agt ttg ggc gaa aac ggg atg tac ctg 1392 Tyr Val 450 ser Asp His Gly Glu 455 Ser Leu Gly Glu Asn 460 Gly Met Tyr Leu cac gcc gcg cct tac gcc atc gcg cct tcc ggg cag acg cat atc ccg 1440 His 465 Ala Ala Pro Tyr Ala 470 Ile Ala Pro Ser Gly 475 Gin Thr His Ile Pro 480 atg gtt atg tgg ttt tcc aaa gcc ttc cgc caa cac ggg ggc ata gat 1488 Met Val Met Trp Phe 485 Ser Lys Ala Phe Arg 490 Gin His Gly Gly Ile 495 Asp ttc caa tgc ctc aaa caa aaa gcg gcg gaa aac gaa tat tcg cac gac 1536 Phe Gin Cys Leu 500 Lys Gin Lys Ala Ala 505 Glu Asn Glu Tyr Ser 510 His Asp cac tat ttc age acg gta ttg ggg ctg atg gac att tcc aac age caa 1584 His Tyr Phe 515 Ser Thr Val Leu Gly 520 Leu Met Asp Ile Ser 525 Asn Ser Gin acc tat cgg aag gaa atg gat ata ttg gca gcc tgc cgc cgt ccg cgc 1632 Thr Tyr Arg Lys Glu Met Asp Ile Leu Ala Ala Cys Arg Arg Pro Arg

530 535 540 <210> 10 <211> 544 <212> PRT <213> Neisseria meningitidis

WO 01/85772 <400> 10

Met Xle Lys Pro Asn Leu Arg Pro Lys Leu Gly 15 10

Ala Phe Leu Ser Leu Tyr Ser Ser Leu Val Leu

20 25 30 • · · · · · · · · · · ···· ···· · · • · · · · · · • ···· ···· • · · · · ··· ···· ···· ·· ··· ·· ·

PCT/GB01/02003

Ser Ser Ala Leu Ile 15

Asn Tvr Ala Phe Phe

Ala Lys Val 35 Val Glu Leu His Pro 40 Phe Asn Gly Thr Gly 45 Ala Asp Ile Phe Leu 50 Tyr Thr Met Pro Val 55 Val Leu Phe Phe Leu 60 Ser Asn Phe Val Phe 65 His Val Ile Ala Leu 70 Pro Phe Val His Lys 75 Val Leu Ile Pro Leu 80 Ile' Leu Val Ile Ser 85 Ala Ala Val Ser Tyr 90 Gin Glu Ile Phe Phe 95 Asn Ile Tyr Phe Asn 100 Lys Ser Met Leu Asn 105 Asn Val Leu Gin Thr 110 Thr Ala Ala Glu Ser 115 Ala Arg Leu Ile Thr 120 Pro Gly Tyr Val Leu 125 Trp Ile Val Cys Leu 130 Gly Val Leu Pro Ala 135 Leu Ala Tyr Ile Ala 140 Val Lys Val Lys Tyr ¹⁴⁵ Arg Val Trp Tyr Lys 150 Glu Leu Leu Thr Arg 155 Leu Val Leu Ala Ala 160 Val Ser Phe Leu Cys 165 Ala Leu Gly Ile Ala 170 Met Leu Gin Tyr Gin 175 Asp Tyr Ala Ser Phe 180 Phe Arg Asn Asn Lys 185 Ser Val Thr His Leu 190 Ile Val Pro Ser Asn 195 Phe Ile Gly Ala Gly 200 Val Ser Lys Tyr Lys 205 Asp Trp Lys Arg Ser 210 Asn Ile Pro Tyr Thr 215 Gin Leu Asp Met Ala 220 Val Val Gin Asn Arg 225 Pro Ala Gly Ser Leu 230 Arg Arg Phe Val Val 235 Leu Val Val Gly Glu 240 Thr Thr Arg Ala Ala Asn Trp Gly Leu Asn Gly Tyr ser Arg Gin Thr

WO 01/85772 PCT/GB01/02003

245 250 255

Thr Pro Leu Leu 260 Ala Ala Arg Gly Asp 265 Glu Ile Val Asn Phe 270 Pro Gin Val Arg Ser 275 Cys Gly Thr Ser Thr 280 Ala His Ser Leu Pro 285 Cys Met Phe Ser Thr 290 Phe Asp Arg Thr Asp 295 Tyr Asp Glu Ile Lys 300 Ala Glu His Gin Asp 305 Asn Leu Leu Asp Ile 310 Val Gin Arg Ala Gly 315 Val Glu Val Thr Trp 320 Leu Glu Asn Asp Ser 325 Gly Cys Lys Gly Val 330 Cys Gly Lys Val Pro 335 Asn Thr Asp Val Thr 340 Ser Leu Asn Leu Pro 345 Glu Tyr Cys Arg Asn 350 Gly Glu Cys Leu Asp 355 Asn Ile Leu Leu Thr 360 Lys Phe Asp Glu Val 365 Leu Asn Lys Asn Asp 370 Lys Asp Ala Val Leu 375 Ile Leu His Thr Ile 380 Gly Ser His Gly Pro 385 Thr Tyr Tyr Glu Arg 390 Tyr Thr Glu Ala Glu 395 Arg Lys Phe Thr Pro 400 Thr Cys Asp Thr Asn 405 Glu Ile Asn Lys Cys 410 Thr Arg Ala Thr Leu 415 Val Asn Thr Tyr Asp 420 Asn Thr Val Leu Tyr 425 Val Asp Gin Phe Ile 430 Asp Lys Val Ile Arg 435 Lys Leu Glu Asn Arg 440 Asp Asp Leu Glu Ser 445 Val Val His Tyr Val 450 Ser Asp His Gly Glu -455 Ser Leu Gly Glu Asn 460 Gly Met Tyr Leu His 465 Ala Ala Pro Tyr Ala 470 Ile Ala Pro Ser Gly 475 Gin Thr His Ile Pro 480 Met Val Met Trp Phe 485 Ser Lys Ala Phe Arg 490 Gin His Gly Gly Ile 495 Asp Phe Gin Cys Leu Lys Gin Lys Ala Ala Glu Asn Glu Tyr Ser His Asp

Ji ,

WO 01/85772 PCT/GB01/02003

500 505 510

His Tyr Phe Ser 515 Thr Val Leu Gly Leu Met Asp Ile Ser Asn Ser Gin 520 525 Thr Tyr Arg Lys Glu Met Asp Ile Leu Ala Ala Cys Arg Arg Pro Arg 530 535 540

<210> 11 <211> 1052 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1050) <400> 11 atg aca cac cat tac ccc aca gac gat att aag att aaa gaa gtt aaa 48

Met Thr His His Tyr Pro Thr Asp Asp Ile Lys Ile Lys Glu Val Lys

1 5 10 15 gag ttg ttg ccg ccg att gcc cat ctt tac gag ctg ccg att tcc aaa 96

Glu Leu Leu Pro Pro Ile Ala His Leu Tyr Glu Leu Pro Ile Ser Lys

20 25 30 gag gct tcg ggc ttg gtt cac cgc acc cgt cag gaa att tcc gat ttg 144

Glu Ala Ser Gly Leu Val His Arg Thr Arg Gin Glu Ile Ser Asp Leu

35 40 45 gtt cac ggc agg gac aag cgg ctg ttg gtt att atc ggg ccg tgt tcg 192

Val His Gly Arg Asp Lys Arg Leu Leu Val Ile Ile Gly Pro Cys Ser

50 55 60 att cac gat ccg aaa gcg gcg ttg gaa tat gcg gag cgt ttg ttg aaa 240

Ile His Asp Pro Lys Ala Ala Leu Glu Tyr Ala Glu Arg Leu Leu Lys

65 70 75 80 ctc cgc aag cag tat gaa aac gag ctt ttg att gtg atg cgc gtt tat 288

Leu Arg Lys Gin Tyr Glu Asn Glu Leu Leu Ile Val Met Arg Val Tyr

85 90 95 ttc gag aag ccg agg acg acg gtg ggt tgg aaa ggt ttg att aac gac 336

Phe Glu Lys Pro Arg Thr Thr Val Gly Trp Lys Gly Leu Ile Asn Asp

100 105 110

WO 01/85772

PCT/GB01/02003

ccg cat ttg gac ggt acg ttt gac Thr Phe Asp 120 atc aat ttc ggt ttg cgt cag gca 384 Pro His Leu Asp Gly 115 Ile Asn Phe Gly Leu 125 Arg Gin Ala cgc agc ctg ttg ttg tcg ctg aac aat atg ggt atg cct gcc tet acc 432 Arg Ser Leu Leu Leu Ser Leu Asn Asn Met Gly Met Pro Ala Ser Thr 130 135 140 gag ttt ttg gat atg att acg ccg caa tat tat gcg gac ttg att tet 480 Glu Phe Leu Asp Met Ile Thr Pro Gin Tyr Tyr Ala Asp Leu Ile Ser 145 150 155 160 tgg ggg gca atc ggc gcg cgg acg acc gaa agc caa gtt cac cgc gaa 528 Trp Gly Ala Ile Gly Ala Arg Thr Thr Glu Ser Gin Val His Arg Glu 165 170 175 ttg gca agc ggg ctg tcc tgc ccc gtc ggc ttt aaa aac ggt acg gat 576 Leu Ala ser Gly Leu Ser Cys Pro Val Gly Phe Lys Asn Gly Thr Asp - . .. 180 185 190 ggc aat ttg aag att gcc atc gac gca atc ggt gcg gcg agc cat tcg 624 Gly Asn Leu Lys Ile Ala Ile Asp Ala Ile Gly Ala Ala Ser His Ser 195 Λ ΛΛ W 205 cat cat ttc ctg tet gta acc aag gcc ggg cat tcc gcc att gtc cat 672 His His Phe Leu Ser Val Thr Lys Ala Gly His Ser Ala Ile Val His 210 215 220 acc ggc ggc aat cct gac tgt cat gtc att ttg cgc ggc gga aaa gag 720 Thr Gly Gly Asn Pro Asp Cys His Val Ile Leu Arg Gly Gly Lys Glu 225 230 235 240 ccg aat tat gat gcg gga cac gtc agc gag gcg gcg gaa caa ctg cgt 768 Pro Asn Tyr Asp Ala Gly His Val Ser Glu Ala Ala Glu Gin Leu Arg 245 250 255 gcg gca ggc gta acc gac aag ctg atg att gat tgc agc cac gcc aac 816 Ala Ala Gly Val Thr Asp Lys Leu Met Ile Asp Cys Ser His Ala Asn 260 265 270 agc cgc aag gat tac acc ega cag atg gaa gtg gcg caa gac att gcc 864 Ser Arg Lys Asp Tyr Thr Arg Gin Met Glu Val Ala Gin Asp Ile Ala 275 280 285 gcc caa ttg gaa cag gac ggc ggc aat att atg ggc gtg atg gtg gaa 912 Ala Gin Leu Glu Gin Asp Gly Gly Asn Ile Met Gly val Met Val Glu 290 295 300

·· ·· ·· · ·· ···· • · · · · · · · · · · • · · · · ···

WO 01/85772 PCT/GB01/02003 age cat ttg gtc gaa ggc agg cag gac aag ccg gaa gtg tac ggc aaa 960 Ser His Leu Val 305 Glu Gly Arg Gin Asp Lys Pro Glu Val Tyr Gly Lys 310 315 320 age att acc gat gcg tgt att ggt tgg gac gcg act gaa gaa ctg ttg 1008 Ser Ile Thr Asp Ala Cys Ile Gly Trp Asp Ala Thr Glu Glu Leu Leu 325 330 335 gca ttg ttg gca Ala Leu Leu Ala 340 <210> 12 <211> 350 <212> PRT <213> Neigseria <400> 12 ggt gca aac aaa aaa cgt atg gcg cgc gcc gg 1052 Gly Ala Asn Lys Lys Arg Met Ala Arg Ala 345 350 τηρηί nni hí Ηί β Met Thr His His 1 Tyr Pro Thr Asp Asp Ile Lys Ile Lys Glu Val Lys 5 10 15 Glu Leu Leu Pro 20 Pro Ile Ala His Leu Tyr Glu Leu Pro Ile Ser Lys 25 30 Glu Ala Ser Gly 35 Leu Val His Arg Thr Arg Gin Glu Ile Ser Asp Leu 40 45 Val His Gly Arg 50 Asp Lys Arg Leu Leu Val Ile Ile Gly Pro Cys Ser 55 60 Ile His Asp Pro 65 Lys Ala Ala Leu Glu Tyr Ala Glu Arg Leu Leu Lys 70 75 . 80 Leu Arg Lys Gin Tyr Glu Asn Glu Leu Leu Ile Val Met Arg Val Tyr 85 90 95 Phe Glu Lys Pro 100 Arg Thr Thr Val Gly Trp Lys Gly Leu Ile Asn Asp 105 110 Pro His Leu Asp 115 Gly Thr'phe Asp Ile Asn Phe Gly Leu Arg Gin Ala 120 125 Arg Ser Leu Leu 130 Leu Ser Leu Asn Asn Met Gly Met Pro Ala Ser Thr 135 140 Glu Phe Leu Asp 145 Met Ile Thr Pro Gin Tyr Tyr Ala Asp Leu Ile Ser 150 155 160

WO 01/85772

PCT/GB01/02003

Trp Gly Ala Ile Gly Ala Arg Thr Thr Glu Ser Gin Val His Arg Glu 165 170 175 Leu Ala Ser Gly Leu Ser Cys Pro Val Gly Phe Lys Asn Gly Thr Asp 180 185 190 Gly Asn Leu Lys Ile Ala Ile Asp Ala Ile Gly Ala Ala Ser His Ser 195 200 205 His His Phe Leu Ser Val Thr Lys Ala Gly His Ser Ala Ile Val His 210 215 220 Thr Gly Gly Asn Pro Asp Cys His Val Ile Leu Arg Gly Gly Lys Glu 225 230 235 240 Pro Asn Tyr Asp Ala Gly His Val Ser Glu Ala Ala Glu Gin Leu Arg • 245 250 255 Ala Ala Gly Val Thr Asp Lys Leu Met Ile Asp Cys Ser His Ala Asn 260 265 270 Ser Ti v»<* • —Ί* T..e rn.._ mu „ nxy Gin Met Glu 'val Ala Gin Asp ile Ala 275 280 285 Ala Gin Leu Glu Gin Asp Gly Gly Asn Ile Met Gly Val Met Val Glu 290 295 300 Ser His Leu Val Glu Gly Arg Gin Asp Lys Pro Glu Val Tyr Gly Lys 305 310 315 320 Ser Ile Thr Asp Ala Cys Ile Gly Trp Asp Ala Thr Glu Glu Leu Leu 325 330 335 Ala Leu Leu Ala Gly Ala Asn Lys Lys Arg Met Ala Arg Ala

340

345

350 <210> 13 <211> 432 <212> DNA <213> Neisseria meningitidis <22 0>

<221> CDŠ <222> (1)..(432)

ϊ

99 9999

9 9

WO 01/85772

PCT/GBO1/02003

9999 9999

9 9 9

9 9 9

9 9 9

9 9 9 9 <400> 13

atg gca ttt ggt Gly tcg atg aat tcc ggc gac gat tet ccg atg tcc gac 48 Met 1 Ala Phe Ser Met 5 Asn Ser Gly Asp 10 Asp Ser Pro Met Ser 15 Asp atc aac gtt acg ccg ttg gtg gac gtg atg ctg gtg ttg ctg att gtg 96 Ile Asn Val Thr Pro Leu Val Asp Val Met Leu Val Leu Leu Ile Val 20 25 30 ttt atg att act atg ccg gtg ctg acg cat tcc atc cct ttg gaa ctg 144 Phe Met Ile Thr Met Pro Val Leu Thr His Ser Ile Pro Leu Glu Leu 35 40 45 ccg acc gcg tcc gag cag aca aac aag cag gac aaa cag cct aaa gac 192 Pro Thr Ala Ser Glu Gin Thr Asn Lys Gin Asp Lys Gin Pro Lys Asp 50 55 60 ccc ctg cgc ctg acg att gat gcg aac ggc ggc tat tat gtc ggc ggg 240 Pro Leu Arg Leu Thr Ile Asp Ala Asn Gly Gly Tyr Tyr Val Gly Gly 65 70 75 80 gat tet gca agc aaa gtg gaa atc ggg gaa gtg gaa agc cgt ctg aaa 288 Asp Ser Ala Ser Lys Val Glu Ile Gly Glu Val Glu Ser Arg Leu Lys 85 90 95 gcc gcc aag gag cag aat gaa aac gtg att gtg gcg att gcg gca gac 336 Ala Ala Lys Glu Gin Asn Glu Asn Val Ile val Ala Ile Ala Ala Asp 100 105 110 aag gcg gtg gaa tac gat tat gta aac aaa gct tta gaa gcc gcc cgt 384 Lys Ala Val Glu Tyr Asp Tyr Val Asn Lys Ala Leu Glu Ala Ala Arg 115 120 125 cag gca gga atc acc aaa atc ggt ttt gta acc gaa acc aag gcg caa 432 Gin Ala Gly Ile Thr Lys Ile Gly Phe Val Thr Glu Thr Lys Ala Gin 130 135 140

<210> 14 <211> 144 <212> PRT <213> Neisseria meningitidis <400> 14

Met Ala Phe Gly Ser Met Asn Ser Gly Asp Asp Ser Pro Met Ser Asp 15 10 15

Ile Asn Val Thr Pro Leu Val Asp Val Met Leu Val Leu Leu Ile Val

WO 01/85772 PCT/GB01/02003 ·· ·· ·· · «··· • · · · · · ·· * · · • ♦ · · · · · 9t • ·««· «··« · • · ··« ··«· ·«·· ···· ·· <·· «« ««

20 25 30

Phe Met Ile 35 Thr Met Pro Val Leu 40 Thr His Ser Ile Pro 45 Leu Glu Leu Pro Thr 50 Ala Ser Glu Gin Thr 55 Asn lys Gin Asp Lys 60 Gin Pro Lys Asp Pro 65 Leu Arg Leu Thr Ile 70 Asp Ala Asn Gly Gly 75 Tyr Tyr Val Gly Gly 80 Asp Ser Ala Ser Lys 85 Val Glu Ile Gly Glu 90 Val Glu Ser Arg Leu 95 Lys Ala Ala Lys Glu 100 Gin Asn Glu Asn Val 105 Ile Val Ala Ile Ala 110 Ala Asp Lys Ala Val 115 Glu Tyr Asp Tyr Val 120 Asn Lys Ala Leu Glu 125 Ala Ala Arg Gin Ala 130 Gly Ile Thr Lys Ile 135 Gly Phe Val Thr Glu 140 Thr Lys Ala Gin

<210> 15 <211> 267 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(267) <400> 15 atg ctc aaa caa tcc atc gaa atc atc aac aaa ctc gga ctc cac gcc 48

Met Leu Lys Gin Ser Ile Glu Ile Ile Asn Lys Leu Gly Leu His Ala .1 5 10 15 cgc gcg tcc aac aag ttc ,acc caa acc gcg tcc caa ttc aaa agc gaa 96

Arg Ala Ser Asn Lys Phe Thr Gin Thr Ala Ser Gin Phe Lys Ser Glu

20 25 30 gtc tgg gtt acg aaa aac gac agc cgc gtc aac ggc aaa agc att atg 144

Val Trp Val Thr Lys Asn Asp Ser Arg Val Asn Gly Lys Ser Ile Met

35 40 45 ggg ctg atg atg ctc gcc gcc gcc aag ggt acg gtc atc gaa ctg gag 192

WO 01/85772 Gly Leu Met Met Leu Ala Ala Ala 50 55 acg gac ggc gcg gac gag gcg gaa Thr Asp Gly Ala Asp Glu Ala Glu 65 70 atc aac ggc tac ttc ggc gag ggc Ile Asn Gly Tyr Phe Gly Glu Gly 85 <210> 16 <211> 89 <212> PRT <213> Neisseria meningitidis <400> 16 Met Leu Lys Gin Ser Ile Glu Ile 1 5 Arg Ala Ser Asn Lys Phe Thr Gin 20 Val Trp Val Thr Lys Asn Asp Ser 35 40 Gly Leu Met Met Leu Ala Ala Ala 50 55 Thr Asp Gly Ala Asp Glu Ala Glu 65 70 Ile Asn Gly Tyr Phe Gly Glu Gly 85

• · · · · ·

• • • • 9 • 9 9 9 • 9 9 9 9 9 9 PCT/GBO1/02003 Lys Gly Thr Val Ile Glu Leu GlU 60 gcg atg cgc gcc ctg acc gac tta 240 Ala Met Arg Ala Leu Thr Asp Leu 75 80

gaa 267

Glu

Ile Asn Lys Leu Gly Leu His Ala 10 15

Thr Ala Ser Gin Phe Lys Ser Glu 25 30

Arg Val Asn Gly Lys Ser Xle Met 45

Lys Gly Thr Val Ile Glu Leu Glu 60

Ala Met Arg Ala Leu Thr Asp Leu 75 80

Glu <210> 17 <211> 633 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(633) <400> 17

WO 01/85772 PCT/GBO1/02003

atg Met 1 ccc atg aac ctt ttt Phe caa aac gcc aaa ttc ttc acg acg gta aac Asn 48 Pro Met Asn Leu 5 Gin Asn Ala Lys 10 Phe Phe Thr Thr Val 15 cat ctc aaa gac ctg ccg gac aca ccg ctc gaa att gcc ttt gtc ggc 96 His Leu Lys Asp Leu Pro Asp Thr Pro Leu Glu Ile Ala Phe Val Gly 20 25 30 agg agc aat gcc gga aaa tcc agt gcc atc aat acc ctg acc aac cat 144 Arg Ser Asn Ala Gly Lys Ser Ser Ala Ile Asn Thr Leu Thr Asn His 35 40 45 gtc cgt ctt gcc tac gtt tca aaa aca ccc gga cgg acg cag cat atc 192 Val Arg Leu Ala Tyr Val Ser Lys Thr Pro Gly Arg Thr Gin His Ile 50 55 60 aac ttc ttc gag ctg cag aac ggc aat ttt atg gtc gat ttg ccc ggc 240 Asn Phe Phe Glu Leu Gin Asn Gly Asn Phe Met Val Asp Leu Pro Gly 65 70 75 80 tac ggt tat gcc caa gtc ccc gaa gca gta cgc gca cat tgg gtc aat 288 Tyr Gly Tyr Ala Gin Val Pro Glu Ala Val Arg Ala His Trp Val Asn 85 °c 95 ctg ctc ggc gac tat ctg caa cag cgc aaa cag ctt atc ggg ctg gtt 336 Leu Leu Gly Asp Tyr Leu Gin Gin Arg Lys Gin Leu Ile Gly Leu Val 100 105 110 ttg att atg gat gcc cgc cat cct tta aaa gaa ctc gac atc cgt atg 384 Leu Xle Met Asp Ala Arg His Pro Leu Lys Glu Leu Asp Xle Arg Met 115 120 125 ctg gac ttt ttc cac acg acc ggc aga ccg gtt cac atc ctg ctg tca 432 Leu Asp Phe Phe His Thr Thr Gly Arg Pro Val His Ile Leu Leu Ser 130 135 140 aaa gcc gac aaa tta tcc aaa aac gaa cag ata aaa acc ctg tcc caa 480 Lys Ala Asp Lys Leu Ser Lys Asn Glu Gin Ile Lys Thr Leu Ser Gin 145 150 155 160 gtc aaa aaa ctg ctc aaa cct tat tcc gac agg caa aac atc agc gta 528 Val Lys Lys Leu Leu Lys Pro Tyr Ser Asp Arg Gin Asn Ile Ser Val 165 170 175 cag ctg ttt tcc agc ctg aaa aaa caa ggt att gac gag gcc aac ega 576 Gin Leu Phe Ser Ser Leu Lys Lys Gin Gly Ile Asp Glu Ala Asn Arg 180 185 190

WO 01/85772

PCT/GB01/02003

act gtc gga age tgg ttt gac gca gca gat gcc gcc get tcc tet Thr Val Gly Ser Trp Phe Asp Ala Ala Asp Ala Ala Ala Ser Ser 195 200 205

cca

Pro

624 aag gaa aac 633

Lys Glu Asn

210 <210> 18 <211> 211 <212> PRT <213> Neisseria meningitidis <400> 18

Met 1 Pro Met Asn Leu 5 Phe Gin Asn Ala Lys Phe Phe Thr 10 Thr Val «1 c Asn His Leu Lys Asp Leu Pro Asp Thr Pro Leu Glu Ile Ala Phe Val Gly 20 25 30 Arg Ser Asn Ala Gly Lys Ser Ser Ala Ile Asn Thr Leu Thr Asn His 35 40 45 Val Arg Leu Ala Tyr Val Ser Lys Thr Pro Gly Arg Thr Gin His Ile 50 55 60 Asn Phe Phe Glu Leu Gin Asn Gly Asn Phe Met Val Asp Leu Pro Gly 65 70 75 80 Tyr Gly Tyr Ala Gin Val Pro Glu Ala Val Arg Ala His Trp val Asn 85 90 95 Leu Leu Gly Asp Tyr Leu Gin Gin Arg Lys Gin Leu Ile Gly Leu Val 100 105 110 Leu Ile Met Asp Ala Arg His Pro Leu Lys Glu Leu Asp Ile Arg Met 115 120 125 Leu Asp Phe Phe His Thr Thr Gly Arg Pro Val His Ile Leu Leu Ser 130 135 140 Lys Ala Asp Lys Leu Ser Lys Asn Glu Gin Ile Lys Thr Leu Ser Gin 145 150 155 160 Val Lys Lys Leu Leu Lys Pro Tyr Ser Asp Arg Gin Asn Ile Ser Val

165 170 175

WO 01/85772 PCT/GB01/02003

Gin Leu Phe Ser Ser Leu Lys Lys Gin Gly Ile Asp Glu Ala Asn Arg 180 185 190

Thr Val Gly Ser Trp Phe Asp Ala Ala Asp Ala Ala Ala Ser Ser Pro 195 200 205

Lys Glu Asn 210 <210> 19 <211> 621 <212> DNA <213> Neisseria meningitidis <22u>

<221> CDS <222> (1)..(621) <400> 19

atg aaa ega ttg act tta ttg gcc ttt gtt Val 10 ttg get gcc ggt gcg gtt 48 Met Lys 1 Arg Leu Thr 5 Leu Leu Ala Phe T.art Ala ř»l .. Jf Ί — 15 V al tcc gca tet ccc aaa gca gac gtg gaa aaa ggc aaa cag gtt gcc gca 96 Ser Ala Ser Pro Lys Ala Asp Val Glu Lys Gly Lys Gin Val Ala Ala 20 25 30 acg gtt tgt gcg get tgc cat gca gca gac ggt aac age ggc att gcg 144 Thr Val Cys Ala Ala Cys His Ala Ala Asp Gly Asn Ser Gly Ile Ala 35 40 45 atg tat ccg cgt ttg gcg gca cag cat act get tac atc tat cat caa 192 Met Tyr Pro Arg Leu Ala Ala Gin His Thr Ala Tyr Ile Tyr His Glh 50 55 60 acc atc ggc atc ege gac ggt aaa ege acc cac ggt tcg gca get gtg 240 Thr Ile Gly Ile Arg Asp Gly Lys Arg Thr His Gly Ser Ala Ala val 65 70 - 75 80 atg aaa ccg gtg gta atg aat ttg age gat cag gat att ttg aac gta 288 Met Lys Pro Val Val Met Asn Leu Ser Asp Gin Asp Ile Leu Asn Val 85 90 95 tcc gca ttc tat gcc aaa cag cag ccc aaa tcc ggt gaa gcc aat cct 336 Ser Ala Phe Tyr Ala Lys Gin Gin Pro Lys Ser Gly Glu Ala Asn Pro 100 105 110

WO 01/85772 PCT/GB01/02003 ··

aag gaa aat ccc gaa ttg ggt Gly gcg aaa atc tat cgc ggc ggt ttg agc 384 Lys Glu Asn 115 Pro Glu Leu Ala Lys 120 Ile Tyr Arg Gly 125 Gly Leu Ser gat aaa aaa gtg ccg gcg tgt atg tcc tgc cac ggt ccg agc ggt gcg 432 Asp Lys Lys Val Pro Ala Cys Met Ser Cys His Gly Pro Ser Gly Ala 130 135 140 ggt atg ccg ggg ggc gga agc gaa att cag gct tat ccg cgt ttg ggc 480 Gly Met Pro Gly Gly Gly Ser Glu Ile Gin Ala Tyr Pro Arg Leu Gly 145 150 155 160 ggt cag cat cag gca tat att gtt gaa cag atg aat gcc tac aag tcc 528 Gly Gin His Gin Ala Tyr Xle Val Glu Gin Met Asn Ala Tyr Lys Ser 165 170 175 ggt cag cgt aaa aat acc atc atg gaa gat att gca aac cgt atg tet 576 Gly Gin Arg Lys Asn Thr Ile Met Glu Asp Ile Ala Asn Arg Met Ser 180 185 190 gaa gaa gat ttg aaa gcg gtc gcc aac ttt atc caa ggt ttg cgt 621 Glu Glu Asp Leu Lys Ala Val Ala Ásn Phe Ile Gin Gly Leu Arg

195 200 205 <210> 20 <211> 207 <212> PRT <213> Neisseria meningitidis <400> 20

Met 1 Lys Arg Leu Thr 5 Leu Leu Ala Phe Val 10 Leu Ala Ala Gly Ala 15 Val Ser Ala Ser Pro 20 Lys Ala Asp Val Glu 25 Lys Gly Lys Gin Val 30 Ala Ala Thr Val Cys 35 Ala Ala Cys His Ala 40 Ala Asp Gly Asn Ser 45 Gly Ile Ala Met Tyr 50 Pro Arg Leu Ala Ala 55 Gin His Thr Ala Tyr 60 Ile Tyr His Gin Thr 65 Ile Gly Ile Arg Asp 70 Gly Lys Arg Thr His 75 Gly Ser Ala Ala Val 80 Met Lys Pro Val Val Met Asn Leu Ser Asp Gin Asp Ile Leu Asn Val

WO 01/85772 PCT/GB01/02003

85 90 95 Ser Ala Phe Tyr 100 Ala Lys Gin Gin Pro 105 Lys Ser Gly Glu Ala 110 Asn Pro Lys Glu Asn 115 Pro Glu Leu Gly Ala 120 Lys Ile Tyr Arg Gly 125 Gly Leu Ser Asp Lys 130 Lys Val Pro Ala Cys 135 Met Ser Cys His Gly 140 Pro Ser Gly Ala Gly 145 Met Pro Gly Gly Gly 150 Ser Glu Ile Gin Ala Tyr 155 Pro Arg Leu Gly 160 Gly Gin His Gin Ala 165 Tyr Ile Val Glu Gin 170 Met Asn Ala Tyr Lys 175 Ser Gly Gin Arg Lys 180 Asn Thr Ile Met Glu 185 Asp Ile Ala Asn Arg 190 Met Ser Glu Glu Asp 195 Leu Lys Ala Val Ala 200 Asn Phe Ile Gin Gly 205 Leu Arg

<210> 21 <211> 765 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1,..(765) <400> 21 atg ccg tet gaa gcc ega ttt ttc agg ctt cag acg gca ttt ccc cgt 48

Met Pro Ser Glu Ala Arg Phe Phe Arg Leu Gin Thr Ala Phe Pro Arg

1 5 10 15 ttg att tgc ggt ata atc .cgc ctt tac cca ttg ttt gca aag cac aat 96

Leu Ile Cys Gly Xle Ile Arg Leu Tyr Pro Leu Phe Ala Lys His Asn

20 25 30 atg aca cgc aaa atc ctc gtt acc tcc gcc ctg ccc tat gcc aac ggc 144

Met Thr Arg Lys Ile Leu Val Thr Ser Ala Leu Pro Tyr Ala Asn Gly

35 40 45 agc atc cac ctc ggc cac atg gtc gaa cac atc caa acc gac gtt tgg 192

WO 01/85772

PCT/GB01/02003

Ser Ile His Leu 50 Gly His Met Val Glu His Ile Gin Thr Asp Val Trp 55 60 gtg cgc ttt caa aaa ctg cgc ggc aac gaa tgc cac tac tgc tgc gcc 240 Val Arg Phe Gin Lys Leu Arg Gly Asn Glu Cys His Tyr Cys Cys Ala 65 70 75 80 gac gac acc cac ggc acg ccc gtg atg ctt gcc gcg caa aaa caa ggc 288 Asp Asp Thr His Gly Thr Pro Val Met Leu Ala Ala Glh Lys Gin Gly 85 90 95 atc gcg ccc gaa gac atg att gcc aaa gtg cgc gaa gag cac ctc gcc 336 Ile Ala Pro Glu Asp Met Ile Ala Lys Val Arg Glu Glu His Leu Ala 100 105 110 gac ttt act ggc ttt ttc atc ggt tac gac aat tat tac age acc cat 384 Ώ er\ DK£ Thr r·^-! i» rv.. f ▼ 1 · vxý Tyr Asp Asn Tyr Tyr Ser Thr His 115 . — 120 125 tcc cct gaa aac aaa cag ttt tcc caa gac att tac cgc gcg ctg aaa 432 Ser Pro Glu Asn Lys Gin Phe Ser Gin Asp Ile Tyr Arg Ala Leu Lys 130 135 140 gcc aac ggc aag att gag age cgc gtc atc gag cag ctt ttc gac ccc 480 Ala Asn Gly Lys Ile GlU Ser Arg Val Ile Glu Gin Leu Phe Asp Pro 145 150 155 160 gaa aaa caa atg ttc ctg ccc gac cgc ttc gtt aaa ggc gaa tgc ccg 528 Glu Lys Gin Met Phe Leu Pro Asp Arg Phe Val Lys Gly Glu Cys Pro 165 170 175 aaa tgc cac gcc caa gac caa tac ggc gac aac tgc gaa gtc tgc ggc 576 Lys Cys His Ala Gin Asp Gin Tyr Gly Asp Asn Cys Glu Val Cys Gly 180 185 190 acg acc tat tcc ccg acc gaa ctg att aac ccg tat tcc gcc gtt tcc 624 Thr Thr Tyr Ser Pro Thr Glu Leu Ile Asn Pro Tyr Ser Ala Val Ser 195 200 205 ggc gcg aaa ccc gaa ttg cgc gaa tcc gaa cac ttc ttc ttc aaa ctg 672 Gly Ala Lys Pro Glu Leu Arg Glu Ser Glu His Phe Phe Phe Lys Leu 210 215 220 gga gaa tgc gcc gac ttc ctc aaa gca tgg act tcc ggc aac aac ccg 720 Gly Glu Cys Ala Asp Phe Leu Lys Ala Trp Thr Ser Gly Asn Asn Pro 225 230 235 240 cac gac ggc aag ccc cat ctg caa gcc gaa gcc ctc aac aaa atg 765

WO 01/85772

PCT/GB01/02003

His Asp Gly Lys Pro His Leu Gin Ala Glu Ala Leu Asn Lys Met 245 250 255 <210> 22 <211> 255 <212> PRT <213> Neisseria meningitidis

<400> 22 Met 1 Pro Ser Glu Ala 5 Arg Phe Phe Arg Leu ¹⁰ Gin Thr Ala Phe Pro 15 Arg Leu Ile Cys Gly 20 ile Ile Arg Leu Tyr 25 Pro Leu Phe Ala Lys 30 His Asn Met Thr Arg 35 Lys Ile Leu val Thr 40 ser Ala Leu Pro Tyr 45 Ala Asn Gly Ser Ile 50 His Leu Gly His Met 55 val Glu His Ile Gin 60 Thr Asp Val Trp Val 65 Arg Phe Gin Lys Leu 70 Arg Gly Asn Glu Cys 75 His Tyr Cys Cys Ala 80 Asp Asp Thr His Gly 85 Thr Pro val Met Leu 90 Ala Ala Gin Lys Gin 95 Gly Ile Ala Pro Glu 100 Asp Met Ile Ala Lys 105 Val Arg Glu Glu His 110 Leu Ala Asp Phe Thr 115 Gly Phe Phe Ile Gly 120 Tyr Asp Asn Tyr Tyr 125 Ser Thr His Ser Pro 130 Glu Asn Lys Gin Phe 135 Ser Gin Asp Ile Tyr 140 Arg Ala Leu Lys Ala 145 Asn Gly Lys Ile Glu 150 Ser Arg Val Ile Glu 155 Gin Leu Phe Asp Pro 160 GlU Lys Gin Met Phe 165 Leu Pro Asp Arg Phe 170 Val Lys Gly Glu Cys 175 Pro Lys Cys His Ala 180 Gin Asp Gin Tyr Gly Asp 185 Asn Cys Glu Val 190 Cys Gly Thr Thr Tyr 195 Ser Pro Thr Glu Leu 200 Ile Asn Pro Tyr Ser 205 Ala Val Ser

WO 01/85772 PCT/GB01/02003

Gly Ala Lys Pro Glu Leu Arg Glu 215 Ser Glu His Phe Phe Phe 220 Lys Leu 210 Gly Glu Cys Ala Asp Phe Leu Lys Ala Trp Thr Ser Gly Asn Asn Pro 225 230 235 240 His Asp Gly Lys Pro His Leu Gin Ala Glu Ala Leu Asn Lys Met 245 250 - 255

<210> 23 <211> 1539 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1539) <400> 23

atg aag ttt tta gac cgt gag gca ágc att gcc aag ccg ggt ttc aac 48 Met 1 Lys Phe Leu Asp Arg 5 Glu Ala Ser Ile 10 Ala Lys Pro Gly Phe 15 Asn cgt tgg ctc gta ccg ccc gca gca ttg gcg gta cac ctt gcc atc ggg 96 Arg Trp Leu Val Pro Pro Ala Ala Leu Ala Val His Leu Ala Ile Gly 20 25 30 cag att tac gcc tat tcc gta ttc aac gcg ccg ctg acc aaa ctt atc 144 Gin Ile Tyr Ala Tyr Ser Val Phe Asn Ala Pro Leu Thr Lys Leu Ile 35 40 45 ggc ata acc gaa tcg gcg gcc gga gat tgg aag ctg acg acc gta ggt 192 Gly Ile Thr Glu Ser Ala Ala Gly Asp Trp Lys Leu Thr Thr Val Gly 50 55 60 tgg att ttc agt atc gca ctc gcg atg ctg ggc gcg tcg gcc gcc ctg 240 Trp Ile Phe Ser Ile Ala Leu Ala Met Leu Gly Ala Ser Ala Ala Leu 65 70 75 80 ttc ggt acg tgg atg gaa cgg gta ggg ccg ege aaa gcc ata ttt gcc 288 Phe Gly Thr Trp Met Glu Arg Val Gly Pro Arg Lys Ala Ile Phe Ala 85 90 95 gcc gcc tgc tgt ttc age ctg ggc ttc ttc gta tcc gca ttc ggc gtg 336 Ala Ala Cys Cys Phe Ser Leu Gly Phe Phe val Ser Ala Phe Gly Val

WO 01/85772

PCT/GB01/02003

100 105 110 cgt acg cac aac ctc ttc ctg ctc tat ttg ggc aac ggc gtc atc ggc 384 Arg Thr His Asn Leu Phe Leu Leu Tyr Leu Gly Asn Gly Val Ile Gly 115 120 125 ggc gta ggc ttg ggt ttg ggc tac atc ggg ccg gtt tcc aca ctg atg 432 Gly Val Gly Leu Gly Leu Gly Tyr Ile Gly Pro Val Ser Thr Leu Met 130 135 140 aaa tgg ttt ccc gac aag ccg ggt atg gcg acc ggt ttg gcg att atg 480 Lys Trp Phe Pro Asp Lys Pro Gly Met Ala Thr Gly Leu Ala Ile Met 145 150 155 160 ggt ttc ggt ggc ggc gca atg ctg gcc tcg ccg ctt tcc gta tca ctg 528 Gly Phe Gly Gly Gly Ala Met Leu Ala Ser Pro Leu Ser Val Ser Leu 165 170 175 atg aac acc ttt tca aac gct act tcg gtt ggg gtt gcc gaa acc ttc 576 Met Asn Thr Phe Ser Asn Ala Thr Ser Val Gly Val Ala Glu Thr Phe 180 185 190 ncr ΓΤ+*» ®--- +-+-ΓΤ ---3 rts-rt* 33” — —a ct** 4- ·» M GjCči čivy ttc ggc gcg ttt 624 Ala Val Leu Gly Leu Leu Tyr Leu Ala Leu Met Met Phe Gly Ala Phe 195 200 205 acc atc cgc gta cct gcc gac ggc tgg aaa cct gaa ggc tat acc gcg 672 Thr Ile Arg Val Pro Ala Asp Gly Trp Lys Pro Glu Gly Tyr Thr Ala 210 215 220 ccg aaa acg čaa aac aag ctg gtc age age aat cat gtc aac gtg tcc 720 Pro Lys Thr Gin Asn Lys Leu Val Ser Ser Asn His Val Asn Val Ser 225 230 235 240 caa gcc atg aaa acg ccg cag ttt tgg ctg ttg ttc tgg gta ttg tgc 768 Gin Ala Met Lys Thr Pro Gin Phe Trp Leu Leu Phe Trp Val Leu Cys 245 250 255 ctg aac gta act gcc ggc atc ggc gta ttg ggt cag gca tcc gtg atg 816 Leu Asn Val Thr Ala Gly Jle Gly Val Leu Gly Gin Ala Ser Val Met 260 265 270 att cag gaa ctc ttt tcc gaa acc tcc ata ggc agg cag gcg gca gtc 864 Ile Gin Glu Leu Phe Ser Glu Thr Ser Ile Gly Arg Gin Ala Ala Val 275 280 285 ggc gca ggt gcg gcg gca ggc ttc gtc age ctc ctg age ctg ttt aac 912 Gly Ala Gly Ala Ala Ala Gly Phe Val Ser Leu Leu Ser Leu Phe Asn

···· ····

WO 01/85772 PCT/GBO1/02003 290 295 300

atg ggc gga cgc ttt ttg tgg Trp tcg Ser age gtt tcc gac aaa atc gga cgc 960 Met 305 Gly Gly Arg Phe Leu 310 Ser Val Ser 315 Asp Lys Ile Gly Arg 320 aaa aac acc tac acc atc ttc ttc gta ctc ggt tcg ctg ctg tat ttc 1008 Lys Asn Thr Tyr Thr Ile Phe Phe Val Leu Gly Ser Leu Leu Tyr Phe 325 330 - 335 gcc gtt ccc tcc atc ggc gag ggc ggc age aaa gcc ctg ttc atc atc 1056 Ala Val Pro Ser Ile Gly GlU Gly Gly Ser Lys Ala Leu Phe Ile Ile 340 345 350 ggc ttt tgc gtg att att tcc atg tac ggc ggc gga ttt gcc gcc att 1104 Gly Phe Cys Val Ile Ile Ser Met Tyr Gly Gly Gly Phe Ala Ala Ile 355 360 365 ccc gcc tac ctg aaa gac ctg ttc ggc acc tat cag gtc ggc gcg att 1152 Pro Ala Tyr Leu Lys Asp Leu Phe Gly Thr Tyr Gin Val Gly Ala Ile 370 375 380 cac gga cgc atc ctg ctg gcc tgg tea acc gcc gcc gtc atc ggc ccg 1200 His Gly Arg Ile Leu Leu Ala Trp šer Thr Ala Ala vai Ile Giy Pro 385 390 395 400 gta ctg gtc aat tac ata cgc caa age caa atc gac age ggc ata ccg 1248 Val Leu Val Asn Tyr Ile Arg Gin Ser Gin Ile Asp Ser Gly Ile Pro 405 410 415 gcg gca cag gca tac age gta acc atg tac atc atg gcg ggt ctg ctg 1296 Ala Ala Gin Ala Tyr Ser Val Thr Met Tyr Ile Met Ala Gly Leu Leu 420 425 430 att atc ggt ttg ttg tgc aac ctc gcc gtc aaa tcc gta cac gaa aaa 1344 Ile Ile Gly Leu Leu cys Asn Leu Ala Val Lys Ser Val His Glu Lys 435 440 445 cac cac gaa aaa gac atc aaa acc gcc gca cac age ggc aac ccc gat 1392 His His Glu Lys Asp Ile Lys Thr Ala Ala His Ser Gly Asn Pro Asp 450 455 460 gac gaa acc gcc ata tcc gac gct tac ctt gtg ggc gaa aaa gtt tcc 1440 Asp Glu Thr Ala Ile Ser Asp Ala Tyr Leu Val Gly Glu Lys Val Ser 465 470 475 480 ggc ggc ggc ata tcg gtc tgg tgg cgt tgg gca ttg gcc gtc ata ccg 1488 Gly Gly Gly Ile Ser Val Trp Trp Arg Trp Ala Leu Ala Val Ile Pro

·« ···· ···· ·· ··· ·· ··

WO 01/85772 PCT/GB01/02003

485 490 495

ctc gcc tac ggc gtg gtg atg atc ttt gtc aaa gca ctc gac Ctt ttc 1536 Leu Ala Tyr Gly Val Val Met Ile Phe Val Lys Ala Leu Asp Leu Phe 500 505 510

tcc 1539

Ser <21O> 24 <211> 513 <212> PRT <213> Neisseria meningitidis <400> 24

1M*· 1 Lys Phe Leu Asp 5 Arg Glu Ala ser ile 10 Ala Lys Pro Gly Phe 15 Asn Arg Trp Leu Val 20 Pro Pro Ala Ala Leu 25 Ala Val His Leu Ala 30 Ile Gly Gin Ile Tyr 35 Ala Tyr Ser Val Phe 40 Asn Ala Pro Leu Thr 45 Lys Leu Ile Gly Ile 50 Thr Glu Ser Ala Ala 55 Gly Asp Trp Lys Leu 60 Thr Thr Val Gly Trp 65 Ile Phe Ser Ile Ala 70 Leu Ala Met Leu Gly 75 Ala Ser Ala Ala Leu 80 Phe Gly Thr Trp Met 85 Glu Arg Val Gly Pro 90 Arg Lys Ala Ile Phe 95 Ala Ala Ala Cys Cys 100 Phe Ser Leu Gly Phe 105 Phe Val Ser Ala Phe 110 Gly Val Arg Thr His 115 Asn Leu Phe Leu Leu 120 Tyr Leu Gly Asn Gly 125 Val Ile Gly Gly Val 130 Gly Leu Gly Leu Gly 135 Tyr Ile Gly Pro Val 140 Ser Thr Leu Met Lys 145 Trp Phe Pro Asp Lys 150 Pro Gly Met Ala Thr 155 Gly Leu Ala Ile Met 160 Gly Phe Gly Gly Gly Ala Met Leu Ala Ser Pro Leu Ser Val Ser Leu

165 170 175

·· ··

WO 01/85772 •· ·94· • · ·

PCT/GB01/02003

Met Asn Thr Phe 180 Ser Asn Ala Thr Ser 185 Val Gly Val Ala Glu 190 Thr Phe Ala Val Leu 195 Gly Leu Leu Tyr Leu 200 Ala Leu Met Met Phe 205 Gly Ala Phe Thr Ile 210 Arg Val Pro Ala Asp 215 Gly Trp Lys Pro Glu 220 Gly Tyr Thr Ala Pro 225 Lys Thr Gin Asn Lys 230 Leu Val Ser Ser Asn 235 His Val Asn Val Ser 240 Gin Ala Met Lys Thr 245 Pro Gin Phe Trp Leu 250 Leu Phe Trp Val Leu 255 Cys Leu Asn Val Thr 260 Ala Gly Ile «1 v --J Val 265 Leu Gly Gin Ala Ser 270 Val Met Ile Gin Glu 275 Leu Phe Ser Glu Thr 280 Ser Ile Gly Arg Gin 285 Ala Ala Val Gly Ala 290 Gly Ala Ala Ala Gly -Phe 295 Val Ser Leu Leu 300 Ser Leu Phe Asn Met 305 Gly Gly Arg Phe Leu 310 Trp Ser Ser Val Ser 315 Asp Lys Ile Gly Arg 320 Lys Asn Thr Tyr Thr 325 Ile Phe Phe Val Leu 330 Gly Ser Leu Leu Tyr 335 Phe Ala Val Pro Ser 340 Ile Gly Glu Gly Gly 345 Ser Lys Ala Leu Phe 350 Ile Ile Gly Phe Cys 355 Val Ile Ile Ser Met 360 Tyr Gly Gly Gly Phe 365 Ala Ala Ile Pro Ala 370 Tyr Leu Lys Asp Leu 375 Phe Gly Thr Tyr Gin 380 Val Gly Ala Ile His 385 Gly Arg Ile Leu Leu 390 'Ala Trp Ser Thr Ala 395 Ala Val Ile Gly Pro 400 Val Leu Val Asn Tyr 405 Ile Arg Gin Ser Gin 410 Ile Asp Ser Gly Ile 415 Pro Ala Ala Gin Ala Tyr Ser Val Thr Met Tyr Ile Met Ala Gly Leu Leu

420 425 430

·· ·· »« • · · · · » · • · · · • · · · · β · · · ··»··««· ·· · ·· ··<»

WO 01/85772 PCT/GB01/02003 Ile Ile Gly 435 Leu Leu Cys Asn Leu 440 Ala Val Lys Ser Val 445 His Glu Lys His His 450 Glu Lys Asp Ile Lys 455 Thr Ala Ala His Ser 460 Gly Asn Pro Asp Asp 465 Glu Thr Ala Ile Ser 470 Asp Ala Tyr Leu Val 475 Gly Glu Lys val Ser 480 Gly Gly Gly Ile Ser 485 Val Trp Trp Arg Trp 490 Ala Leu Ala Val Ile 495 Pro Leu Ala Tyr Gly 500 Val Val Met Ile Phe 505 Val Lys Ala Leu Asp 510 Leu Phe

Ser <210> 25 <211> 1038 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1038) <400> 25

ttg cag cct tta gtc age gta ttg att tgc gcc tac aac gta gaa aaa 48 Leu 1 Gin Pro Leu Val 5 Ser Val Leu Ile Cys 10 Ala Ťyr Asn Val Glu 15 Lys tat ttc gcc caa tea tta gcc acc gtc gtg aat cag act tgg cgc aac 96 Tyr Phe Ala Gin Ser Leu Ala Thr val Val Asn Gin Thr Trp Arg Asn 20 25 30 ttg gag att ttg att gtc gat gac ggc tcg aca gac ggt acg ctt gcc 144 Leu Glu Ile Leu Ile Val Asp Asp Gly Ser Thr Asp Gly Thr Leu Ala 35 40 45 att gcc aag gat ttt caa aag cgg gac age cgt atc aaa atc ctt gca 192 Ile Ala Lys Asp Phe Gin Lys Arg Asp Ser Arg Ile Lys Ile Leu Ala 50 55 60 caa get caa aat tcc ggc ctg att ccc tet tta aac atc ggg ctg gac 240

·· ·· • · • · β β ········ »·· • · · • * · • · · · • · · · ·· ··

WO 01/85772 PCT/GB01/02003 Gin Ala Gin Asn Ser Gly Leu Ile Pro Ser Leu Asn Ile Gly Leu Asp 65 70 75 80 gaa ttg gca aag tca gga atg ggg gaa tat att gca cgc acc gat gcc 288 Glu Leu Ala Lys Ser Gly Met Gly Glu Tyr Ile Ala Arg Thr Asp Ala 85 90 95 gac gat att gcc gcc ccc gac tgg att gag aaa atc gtg ggc gag atg 336 Asp Asp Ile Ala Ala Pro Asp Trp Ile Glu Lys Ile Val Gly Glu Met 100 105 110 gaa aaa gac cgc agc atc atc gcg atg ggc gcg tgg ctg gaa gtt ttg 384 Glu Lys Asp Arg Ser Ile Ile Ala Met Gly Ala Trp Leu Glu Val Leu 115 120 125 tcg gaa gaa aag gac ggc aac cgg ctg gcg cgg cat cac agg cac ggc 432 Ser Glu Glu *-»**í* Gly Asn n «.m λχ y T ucu ma Arg His His Arg His Gly 130 135 140 aaa att tgg aaa aag ccg acc cgg cac gaa gat att gcc gac ttt ttc 480 Lys Ile Trp Lys Lys Pro Thr Arg His Glu Asp Ile Ala Asp Phe Phe 145 150 155 160 cct ttc ggc aac ccc ata cac aac aac acg atg att atg cgg cgc agc 528 Pro Phe Gly Asn Pro Ile His Asn Asn Thr Met Ile Met Arg Arg Ser 165 170 175 gtc att gac ggc gga ttg cgt tac aac acc gag cgg gat tgg gcg gaa 576 Val Ile Asp Gly Gly Leu Arg Tyr Asn Thr Glu Arg Asp Trp Ala Glu 180 185 190 gat tac caa ttt tgg tac gat gtc agc aaa ttg ggc agg ctg gct tat 624 Asp Tyr Gin Phe Trp Tyr Asp Val Ser Lys Leu Gly Arg Leu Ala Tyr 195 200 205 tat ccc gaa gcc ttg gtc aaa tac cgc ctt cac gcc aat cag gtt tca 672 Tyr Pro Glu Ala Leu Val Lys Tyr Arg Leu His Ala Asn Gin Val Ser 210 215 220 tcc aaa tac agc atc cgc caa cac gaa atc gcg caa ggc atc caa aaa 720 Ser Lys Tyr Ser Ile Arg Gin His Glu Ile Ala Gin Gly Ile Gin Lys 225 230 235 240 acc gcc aga aac gat ttt ttg cag tet atg ggt ttt aaa acc cgg ttc 768 Thr Ala Arg Asn Asp Phe Leu Gin Ser Met Gly Phe Lys Thr Arg Phe 245 250 255 gac agc ctt gaa tac cgc caa ata aaa gca gta gcg tat gaa ttg ctg 816

WO 01/85772 PCT/GB01/02003

Asp Ser Leu Glu 260 Tyr Arg Gin Ile Lys Ala Val Ala Tyr Glu Leu Leu 265 270 gag aaa cat ttg ccg gaa gaa gat ttt gaa cgc gcc cgc cgg ttt ttg 864 Glu Lys His Leu Pro Glu Glu Asp Phe Glu Arg Ala Arg Arg Phe Leu 275 280 285 tac caa tgc ttc aaa cgg acg gac acg ccg ccc gcc ggp gcg tgg ctg 912 Tyr Gin Cys Phe Lys Arg Thr Asp Thr Pro Pro Ala Gly Ala Trp Leu 290 295 300 gat ttc gcg gca gac ggc aag atg agg cgg ctg ttt acc atg agg caa 960 Asp Phe Ala Ala Asp Gly Lys Met Arg Arg Leu Phe Thr Met Arg Gin 305 310 315 320 tac ttc ggc att ttg cac cgg ctg att aaa aac cgc cgg cag gcg cgg 1008 Tyr Phe Gly Ile Leu His Arg Leu Ile Lys Asn Arg Arg Gin Ala Arg 325 330 335 tcg gat tcg gca ggg aaa gaa cag gag att 1038 Ser Asp Ser Ala Gly Lys Glu Gin Glu Ile 340 345

<21O> 26 <211> 346 <212> PRT <213> Neisseria meningitidis <400> 26

Leu Gin Pro Leu Val Ser Val Leu Ile Cys Ala Tyr Asn Val Glu Lys

1 5 10 15

Tyr Phe Ala Gin Ser Leu Ala Thr Val Val Asn Gin Thr Trp Arg Asn

20 25 30

Leu Glu Ile Leu Ile Val Asp Asp Gly Ser Thr Asp Gly Thr Leu Ala 35 40 45

Ile Ala Lys Asp Phe Gin Lys Arg Asp Ser Arg Ile Lys Ile Leu Ala 50 55 60

Gin Ala Gin Asn Ser Gly Leu Ile Pro Ser Leu Asn Ile Gly Leu Asp 65 70 75 80

Glu Leu Ala Lys Ser Gly Met Gly Glu Tyr Ile Ala Arg Thr Asp Ala 85 90 95

WO 01/85772 PCT/GB01/02003

Asp Asp Ile Ala 100 Ala Pro Asp Trp Ile Glu Lys Ile Val Gly Glu Met 105 110 Glu Lys Asp Arg Ser Ile Ile Ala Met Gly Ala Trp Leu Glu Val Leu 115 120 125 Ser Glu Glu Lys Asp Gly Asn Arg Leu Ala Arg His His Arg His Gly 130 135 140 - Lys Ile Trp Lys Lys Pro Thr Arg His Glu Asp Ile Ala Asp Phe Phe 145 150 155 160 Pro Phe Gly Asn Pro Ile His Asn Asn Thr Met Ile Met Arg Arg Ser 165 170 175 Val Ile Asp Gly Gly Leu Arg Tyr Asn Thr Glu Arg Asp Trp Ala Glu 180 185 190 Asp Tyr Gin Phe Trp Tyr Asp Val Ser Lys Leu Gly Arg Leu Ala Tyr 195 200 205 Tyr Pro Glu Ala Leu Val Lys Tyr Arg Leu His Ala Asn Gin Val Ser 210 215 220 Ser Lys Tyr Ser Ile Arg Gin His Glu Ile Ala Gin Gly Ile Gin Lys 225 230 235 240 Thr Ala Arg Asn Asp Phe Leu Gin Ser Met Gly Phe Lys Thr Arg Phe 245 250 255 Asp Ser Leu Glu Tyr Arg Gin Ile Lys Ala Val Ala Tyr Glu Leu Leu 260 265 270 Glu Lys His Leu Pro Glu Glu Asp Phe Glu Arg Ala Arg Arg Phe Leu 275 280 285 Tyr Gin Cys Phe Lys Arg Thr Asp Thr Pro Pro Ala Gly Ala Trp Leu 290 295 300 Asp Phe Ala Ala Asp Gly Lys Met Arg Arg Leu Phe Thr Met Arg Gin 305 310 315 320 Tyr Phe Gly Ile Leu His Arg Leu Ile Lys Asn Arg Arg Gin Ala Arg 325 330 335 Ser Asp Ser Ala Gly Lys Glu Gin Glu Ile 340 345

® · ··· ···· ···· ···· ·· ··· _ee

WO 01/85772 PCT/GB01/02003 <210> 27 <211> 1856 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1857) <400> 27

atg Met 1 cca gaa tac Tyr cgc tcc aaa acc tcc acc cac ggt cgc aat atg gcg 48 Pro Glu Arg 5 Ser Lys Thr Ser Thr 10 His Gly Arg Asn Met 15 Ala ggc gcg cgc gcg ttg tgg cgc gcc acc ggc gtg atg gaa acc gac ttc 96 Gly Ala Arg Ala Leu Trp Arg Ala Thr Gly Val Met Glu Thr Asp Phe 20 25 30 ggc aaa ccg att atc gcc gtt gcc aac tcg ttc acc caa ttc gtg cca 144 Gly Lys Pro Ile Ile Ala Val Ala Asn Ser Phe Thr Gin Phe val Pro 35 40 45 ggt cat gtc cac ctg cac aat atg ggc cag ctg gtt gcc cgc gaa atc 192 Gly His Val His Leu His Asn Met Gly Gin Leu Val Ala Arg Glu Ile 50 55 60 gaa aaa gcc ggc gcc atc gcc aaa gaa ttc aac acc atc gcc atc gac 240 Glu Lys Ala Gly Ala Ile Ala Lys Glu Phe Asn Thr Ile Ala Ile Asp 65 70 75 80 gac ggc atc gct atg gga cac agc ggt atg ctg tat tcc ctg ccc agc 288 Asp Gly Ile Ala Met Gly His Ser Gly Met Leu Tyr Ser Leu Pro Ser 85 90 95 cgc gat ttg att gcc gac tcc atc gaa tat atg gtc aat gcc cac tgc 336 Arg Asp Leu Ile Ala Asp Ser Ile Glu Tyr Met Val Asn Ala His Cys 100 105 110 gcc gac gcg ctg gtg tgc att tcc aac tgc gac aaa atc acc ccg ggc 384 Ala Asp Ala Leu Val Cys Ile Ser Asn Cys Asp Lys Ile Thr Pro Gly 115 120 125 atg ctg att gcc gcg atg cgc ctg aac atc ccg acc atc ttc gtt tcc 432 Met Leu Ile Ala Ala Met Arg Leu Asn Ile Pro Thr Ile Phe Val Ser 130 135 140

WO 01/85772 • fr frfr • · · · • · • · • . · ··«« frfrfrfr • fr frfrfrfr • · · • frfr fr·· e • ·· · • fr ··

PCT/GB01/02003

ggc ggt ccg atg gaa gca ggt aaa gtt atc ggc gtg gca aac atc cag Gin 160 480 Gly 145 Gly Pro Met Glu Ala 150 Gly Lys Val Ile Gly 155 Val Ala Asn Ile ccc gaa cgc cgt ttg gac ttg att gac gcg atg att gaa tcg gcg gac 528 Pro Glu Arg Arg Leu Asp Leu Ile Asp Ala Met Ile Glu Ser Ala Asp 165 170 175 gac aat atc age aac cgg caa gtt gaa gaa gtc gaa caa aac gcc tgc 576 Asp Asn Ile Ser Asn Arg Gin Vál Glu Glu Val Glu Gin Asn Ala Cys 180 185 190 ccg acc tgc ggc tcg tgt tcg ggt atg ttt acg gca aac tcg atg aac 624 Pro Thr Cys Gly Ser Cys Ser Gly Met Phe Thr Ala Asn Ser Met Asn 195 200 205 tgc ctg acc gaa gca ctc ggg ctg tcc ctg ccg ggc aac ggt tet tac 672 Cys Leu Thr Glu Ala Leu Gly Leu Ser Leu Pro Gly Asn Gly Ser Tyr 210 215 220 ctc 'gcc acc cac gtc ggc cgc aaa gaa ctg ttc ctc gaa gcc ggc cgt 720 Leu Ala Thr His Val Gly Arg Lys Glu Leu Phe Leu Glu Ala Gly Arg 225 230 235 240 atg att .gtc gaa atc acc aaa cgc tat tac gag caa aac gat gaa acc 768 Met Ile Val Glu Ile Thr Lys Arg Tyr Tyr Glu Gin Asn Asp Glu Thr 245 250 255 gtg tta ccg cgc age att gcc acc aaa aaa gcg ttt gaa aac get atg 816 Val Leu Pro Arg Ser Ile Ala Thr Lys Lys Ala Phe Glu Asn Ala Met 260 265 270 acc atg gac att gcc atg ggc ggc age acc aac act att ttg cat tta 864 Thr Met Asp Ile Ala Met Gly Gly Ser Thr Asn Thr Ile Leu His Leu 275 280 285 ctc gcc gtt gcc aac gaa gcg ggc gtg gat ttc aaa atg gca gac atc 912 Leu Ala Val Ala Asn Glu Ala Gly Val Asp Phe Lys Met Ala Asp Ile 290 295 300 gac cgc tta age cgc gtt gtg ccc tgc atc tgc aaa acc gca ccc aac 960 Asp Arg Leu Ser Arg Val Val Pro Cys Ile Cys Lys Thr Ala Pro Asn 305 310 315 320 aac cac gac tac tat atg gaa gac gtg cac ega gcc ggc ggc atc ttc 1008 Asn His Asp Tyr Tyr Met Glu Asp Val His Arg Ala Gly Gly Ile Phe 325 330 335

WO 01/85772 ·· 44 • 4 9 4

4 9

4 4 4

4 9

4444 4444 ·· • ·« ·

9 4 4 • 4 4

4 4 9 « • · · 9 • 44 4·

PCT/GB01/02003

gcc Ala atc ctg aaa gaa ttg gac aaa gcg ggc aag ctg cac acc gac Thr Asp 350 gtg Val 1056 Ile Leu Lys 340 Glu Leu Asp Lys Ala Gly 345 Lys Leu His cac act atc cac gcg ccg acg ctg aaa gac gcg att gaa aaa tgg gac 1104 His Thr Ile His Ala Pro Thr Leu Lys Asp Ala Ile Glu Lys Trp Asp 355 360 365 gta acc aac cct gaa aac acc cac gcc atc gaa cgc ttc aaa gcc gcg 1152 Val Thr Asn Pro Glu Asn Thr His Ala Ile Glu Arg Phe Lys Ala Ala 370 375 380 ccc ggc ggc gta cgc acc acc caa gcg ttc tcg caa aac cgt atg tgg 1200 Pro Gly Gly Val Arg Thr Thr Gin Ala Phe Ser Gin Asn Arg Met Trp 385 390 395 400 aaa acc ctc gac ctc gac cgc gaa aaa ggc tgt atc cgc gac gtg gca 1248 Lys Thr Leu Asp Leu Asp Arg Glu Lys Gly Cys Ile Arg Asp Val Ala 405 410 415 cac gcc tac tcg caa gac ggc ggt ttg gcg gtc ttg ttc ggc aac atc 1296 His Ala Tyr Ser Gin Asp Gly Gly Leu Ala Val Leu Phe Gly Asn Ile 420 425 430 gcc gag cgc ggc tgc gtg gta aaa acc gca ggc gtg gac gag agc atc 1344 Ala Glu Arg Gly Cys Val Val Lys Thr Ala Gly Val Asp Glu Ser Ile 435 440 445 ctc aaa ttc acc ggc cgc gcc cgc gtg ttt gaa agc caa gaa gac gca 1392 Leu Lys Phe Thr Gly Arg Ala Arg Val Phe Glu Ser Gin Glu Asp Ala 450 455 460 gta gaa ggc att ttg ggc aac caa atc gtc gcc ggc gac atc gtc atc 1440 Val Glu Gly Ile Leu Gly Asn Gin Ile Val Ala Gly Asp Ile Val Ile 465 470 475 480 atc cgc tac gaa ggc ccg aaa ggc ggc ccg ggc atg cag gaa atg ctg 1488 Ile Arg Tyr Glu Gly Pro Lys Gly Gly Pro Gly Met Gin Glu Met Leu 485 490 495 tat ccg act tcc tac ctg aaa tet aaa ggc ctc ggc aaa gcc tgc gct 1536 Tyr Pro Thr Ser Tyr Leu Lys Ser Lys Gly Leu Gly Lys Ala Cys Ala 500 505 510 ctc tta acc gac gga cgc ttc tcc ggc ggc aca tea ggt ttg tcc atc 1584 Leu Leu Thr Asp Gly Arg Phe Ser Gly Gly Thr Ser Gly Leu Ser Ile 515 520 525

WO 01/85772 PCT/GB01/02003 gga cac gcc tcg ccc gaa gcg gcg gaa ggt ggc gcg atc ggt ttg gta 1632 Gly His Ala Ser Pro Glu Ala Ala Glu Gly Gly Ala Ile Gly Leu Val 530 535 540 cac gaa ggc gat acc gtc gaa atc gac atc ccc aac cgc agc atc cgc 1680 His Glu Gly Asp Thr Val Glu Ile Asp Ile Pro Asn Arg Ser Ile Arg 545 550 555 560 ctt gtc att tcc gac gaa gaa ctc gcc gcc cgc cgt gcc gaa atg gaa 1728 Leu Val Ile Ser Asp Glu Glu Leu Ala Ala Arg Arg Ala Glu Met Glu 565 570 575 gca cgc ggc agc aaa gcg tgg aaa ccg gaa aac cgc gac cgc tac gtc 1776 Ala Arg Gly Ser Lys Ala Trp Lys Pro Glu Asn Arg Asp Arg Tyr Val 580 585 590 tcc gcc gca tta aga gct tac ggc gcg atg gcg act tcc gcc gac aaa 1824 Ser Ala Ala Leu Arg Ala Tyr Gly Ala Met Ala Thr Ser Ala Asp Lys 595 600 605 ggc gcg gtg cgc gac gta gcg caa atc gaa aga 1857 Gly Ala Val Arg Asp Val Ala Gin Ile Glu Arg 610 615 -

<210> 28 <211> 619 <212> PRT <213> Neisseria meningitidis <400> 28

Met 1 Pro Glu Tyr Arg 5 Ser Lys Thr Ser Thr 10 His Gly Arg Asn Met 15 Ala Gly Ala Arg Ala 20 Leu Trp Arg Ala Thr 25 Gly Val Met Glu Thr 30 Asp Phe Gly Lys Pro 35 Ile Ile Ala Val Ala 40 Asn Ser Phe Thr Gin 45 Phe Val Pro Gly His 50 Val His Leu His Asn 55 Met Gly Gin Leu Val 60 Ala Arg Glu Ile Glu 65 Lys Ala Gly Ala Ile Ala 70 Lys Glu Phe Asn 75 Thr Ile Ala Ile Asp 80 Asp Gly Ile Ala Met Gly His Ser Gly Met Leu Tyr Ser Leu Pro Ser

85 90 95

WO 01/85772

PCT/GB01/02003

Arg Asp Leu Ile 100 Ala Asp Ser Ile Glu 105 Tyr Met Val Asn Ala 110 His Cys Ala Asp Ala 115 Leu Val Cys Ile Ser 120 Asn Cys Asp Lys Ile 125 Thr Pro Gly Met Leu 130 Ile Ala Ala Met Arg 135 Leu Asn Ile Pro Thr 140 Ile Phe val Ser Gly 145 Gly Pro Met Glu Ala 150 Gly Lys Val Ile Gly 155 Val Ala Asn Ile Gin 160 Pro Glu Arg Arg Leu 165 Asp Leu Ile Asp Ala 170 Met Ile Glu Ser Ala 175 Asp Asp Asn Ile Ser 180 Asn Arg Gin Val Glu 185 Glu Val Glu Gin Asn 190 Ala Cys Pro Thr Cys 195 Gly Ser Cys Ser Gly 200 Met Phe Thr Ala Asn 205 Ser Met Asn cys Leu 210 Thr. Glu Ala Leu Gly 215 Leu Ser Leu Pro Gly 220 Asn Gly Ser Tyr Leu 225 Ala Thr His Val Gly Arg 230 Lys Glu Leu Phe 235 Leu Glu Ala Gly Arg 240 Met Ile Val Glu Ile 245 Thr Lys Arg Tyr Tyr 250 Glu Gin Asn Asp Glu 255 Thr Val Leu Pro Arg 260 Ser Ile Ala Thr Lys 265 Lys Ala Phe Glu Asn 270 Ala Met Thr Met Asp 275 Ile Ala Met Gly Gly 280 Ser Thr Asn Thr Ile 285 Leu His Leu Leu Ala 290 Val Ala Asn Glu Ala 295 Gly Val Asp Phe Lys 300 Met Ala Asp Ile Asp 305 Arg Leu Ser Arg Val 310 Val Pro cys Ile Cys 315 Lys Thr Ala Pro Asn 320 Asn His Asp Tyr Tyr 325 Met Glu Asp Val His 330 Arg Ala Gly Gly Ile 335 Phe Ala Ile Leu Lys 340 Glu Leu Asp Lys Ala 345 Gly Lys Leu His Thr 350 Asp Val

WO 01/85772

PCT/GB01/02003

His Thr Ile 355 His Ala Pro Thr Leu 360 Lys Asp Ala Ile Glu 365 Lys Trp Asp Val Thr 370 Asn Pro Glu Asn Thr 375 His Ala Ile Glu Arg 380 Phe Lys Ala Ala Pro 385 Gly Gly Val Arg Thr 390 Thr Gin Ala Phe Ser 395 Gin Asp Arg Met Trp 400 Lys Thr Leu Asp Leu 405 Asp Arg Glu Lys Gly 410 Cys Ile Arg Asp Val 415 Ala His Ala Tyr Ser 420 Gin Asp Gly Gly Leu 425 Ala Val Leu Phe Gly 430 Asn Ile Ala Glu Arg 435 Gly Cys Val Val Lys 440 Thr Ala Gly Val Asp 445 Glu Ser Ile Leu Lys 450 Phe Thr Gly Arg Ala 455 Arg Val Phe Glu Ser 460 Gin Glu Asp Ala Val 465 .. WJ. VA Π .. Ile Leu Gly 470 Asn Gin Ile val Ala 475 Gly Asp Ile Val Ile 480 Ile Arg Tyr Glu Gly 485 Pro Lys Gly Gly Pro 490 Gly Met Gin Glu Met 495 Leu Tyr Pro Thr Ser 500 Tyr Leu Lys Ser Lys 505 Gly Leu Gly Lys Ala 510 Cys Ala Leu Leu Thr 515 Asp Gly Arg Phe Ser 520 Gly Gly Thr Ser Gly 525 Leu Ser Ile Gly His 530 Ala Ser Pro Glu Ala .535 Ala Glu Gly Gly Ala 540 Ile Gly Leu Val His 545 Glu Gly Asp Thr Val 550 Glu Ile Asp Ile Pro 555 Asn Arg Ser Ile Arg 560 Leu Val Ile Ser Asp 565 Glu Glu Leu Ala Ala 570 Arg Arg Ala Glu Met 575 Glu Ala Arg Gly Ser 580 Lys Ala Trp Lys Pro 585 Glu Asn Arg Asp Arg 590 Tyr Val Ser Ala Ala 595 Leu Arg Ala Tyr Gly 600 Ala Met Ala Thr Ser 605 Ala Asp Lys

WO 01/85772 PCT/GBO1/02003

Gly Ala Val Arg Asp Val Ala Gin Ile Glu Arg 610 615 <210> 29 <211> 1725 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1725) <400 29

atg Met 1 cag cta tca ggc gcg caa atc ata gtg cag agt ctc aaa gcc gaa 48 Gin Leu Ser Gly 5 Ala Gin Ile Ile Val 10 Gin Ser Leu Lys Ala 15 Glu ggt gtc gag tac gtt ttc ggt tat ccc ggc ggt gcg gtt atc gaa atc 96 Gly Val Glu Tyr Val Phe Gly Tyr Pro Gly Gly Ala Val Ile Glu Ile 20 25 30 tac gat gcc ctt ttc caa ctc aat aaa ttc aag cac att ctg acc cgt 144 Tyr Asp Ala Leu Phe Gin Leu Asn Lys Phe Lys His Ile Leu Thr Arg 35 40 45 cac gag cag gcg gca gta cac gcg gca gat gcg tat gcg cgc gtc age 192 His Glu Gin Ala Ala Val His Ala Ala Asp Ala Tyr Ala Arg Val Ser 50 55 60 ggt aag gtg ggc gtg gca ttg gtt aca tcc ggc ccg ggc gtt acc aat 240 Gly Lys val Gly Val Ala Leu Val Thr Ser Gly Pro Gly Val Thr Asn 65 70 75 80 gca ctg acc ggt att get act gcc tat acg gat tcg att ccg atg gtg 288 Ala Leu Thr Gly Ile Ala Thr Ala Tyr Thr Asp Ser Ile Pro Met Val 85 90 95 gtc atc age ggg cag gta ggc aat tcc ctg att ggt acg gat gcg ttc 336 val Ile Ser Gly Gin Val Gly Asn Ser Leu Ile Gly Thr Asp Ala Phe 100 105 110 caa gaa gtt gat acg gtg ggt att acc cgt ccg tgc gtc aaa cac aat 384 Gin Glu Val Asp Thr Val Gly Ile Thr Arg Pro Cys Val Lys His Asn

115 120 125 »· ·» · · • · · · · ·

PCT/GB01/02003 atc aat gag ttg gcg gaa acc att aaa aag gcg 432

Ile Asn Glu Leu Ala Glu Thr Ile Lys Lys Ala 135 140 age ggc ega ccg ggg ccc gtg gtg gtt gat gtc 480

WO 01/85772 ttc ctg gtt acg gac Phe Leu Val Thr Asp 130 ttc caa att gcc gca

Phe Gin Ile Ala Ala Ser Gly Arg Pro Gly Pro Val Val Val Asp Val 145 150 155 160

ccg Pro aaa gat gtt acg cag gcg atg Ala Met gcg Ala aaa ttc age tat cct cag gaa 528 Lys Asp Val Thr 165 Gin Lys 170 Phe Ser Tyr Pro Gin 175 Glu gac att ttt atc cgt tcg tat caa ccg gtt gtt caa ggg cat atc ggt 576 Asp Ile Phe Ile Arg Ser Tyr Gin Pro Val Val Gin Gly His Ile Gly 180 185 190 cag att aaa aag gcc gtg cag atg ttg gca tet gcc aaa cgt ccg gtc 624 Gin Ile Lys Lys Ala Val Gin Met Leu Ala Ser Ala Lys Arg Pro Val 195 200 205 gtc tat ttt ggc ggc ggc gtg gtg ttg ggt aat get tet gaa gag ctg 672 Val Tyr Phe Gly Gly Gly Val Val Leu Gly Asn Ala Ser Glu Glu Leu 210 215 *»o n - - acc ega ttt gtc ega atg acg ggt get ccg tgc acg ggt acg ctg atg 720 Thr Arg Phe Val Arg Met Thr Gly Ala Pro Cys Thr Gly Thr Leu Met 225 230 235 240 ggc ttg ggc get tat cct tcc ggc gac ege caa ttc ctc ggt atg ctc 768 Gly Leu Gly Ala Tyr Pro Ser Gly Asp Arg Gin Phe Leu Gly Met Leu 245 250 255 ggt atg cac ggt act tac gag gca aac ctt gcc atg cag aat gcg gat 816 Gly Met His Gly Thr Tyr Glu Ala Asn Leu Ala Met Gin Asn Ala Asp 260 265 270 gtc gtt ctt gcc gta ggt gcg cgt ttt gac gac cgt gtg gtt tcc gta 864 Val Val Leu Ala Val Gly Ala Arg Phe Asp Asp Arg Val Val Ser Val 275 280 285 ccg tcc aaa ttt ttc gag aag gcg aag aag gtt atc cat atc gat gtc 912 Pro Ser Lys Phe Phe Glu Lys Ala Lys Lys Val Ile His Ile Asp Val 290 295 300 gat cct tcc age atc gcc aaa ege gtg aag gcg gac att ccg att gtc 960 Asp Pro Ser Ser Ile Ala Lys Arg Val Lys Ala Asp Ile Pro Ile Val

305 3io 315 320

WO 01/85772

PCT/GB01/02003

ggc gac gtg aaa aac gtt ttg tcc gag atg gtt gcg ctg tgg caa aaa 1008 Gly Asp Val Lys Asn Val 325 Leu Ser Glu Met 330 Val Ala Leu Trp Gin 335 Lys caa gag tcc gtg ccg tet gaa gat get ttg ggc aaa tgg tgg aaa acc 1056 Gin Glu Ser Val Pro Ser Glu Asp Ala Leu Gly Lys Trp Trp Lys Thr 340 345 - 350 ata gag gaa tgg cgt tcc cgc gat tgc ttg tgg ttt gac aac ggc age 1104 Ile Glu Glu Trp Arg Ser Arg Asp Cys Leu Trp Phe Asp Asn Gly Ser 355 360 365 gaa att atc aag cca caa tat gtg att cag aag ctt gcc gag att acc 1152 Glu Ile Ile Lys Pro Gin Tyr Val Ile Gin Lys Leu Ala Glu Ile Thr 370 375 380 ggc aat tcg gca atc atc aca tcg gat gta ggg cag cat caa atg ttt 1200 Gly Asn Ser Ala Ile Ile Thr Ser Asp Val Gly Gin His Gin Met Phe ....... 385 390 395 400 gcg get caa tat tat ccc ttc gaa cgt ccg cgc caa tgg ctc aac tcc 1248 Ala Ala Gin Tyr Tyr Pro Phe Glu ZXrrr ' ~~~ 9 Pro Arrr - — 9 Gin • T Leu As** Ser 405 410 415 ggc ggt ttg ggt acg atg ggc gtt ggt ctg cct tat gcg ' att ggt gca 1296 Gly Gly Leu Gly Thr Met Gly Val Gly Leu Pro Tyr Ala Ile Gly Ala 420 425 430 aaa ctt gcc gcc ccg gat cáa gac gta ttc tgt att acc ggc gac ggc 1344 Lys Leu Ala Ala Pro Asp Gin Asp Val Phe Cys Ile Thr Gly Asp Gly 435 440 445 tcg atc cag atg aac atc caa gag ctg tcc acc tgt ttc caa tat cgg 1392 Ser Ile Gin Met Asn Ile Gin Glu Leu Ser Thr Cys Phe Gin Tyr Arg 450 455 460 att ccg gtt aac gtc att acg ctg aac aac ggt tat ctc ggt atg gta 1440 Ile Pro Val Asn Val Ile Thr Leu Asn Asn Gly Tyr Leu Gly Met Val 465 470 475 480 cgc cag tgg cag gaa ata tat tac ggc gga ega gag tcg gaa acc tat 1488 Arg Gin Trp Gin Glu Ile Tyr Tyr Gly Gly Arg Glu Ser Glu Thr Tyr 485 490 495 ttc gat tet ttg ccc gat ttc gtc aaa ctt gcc gag gca tac ggc cat 1536 Phe Asp Ser Leu Pro Asp Phe Val Lys Leu Ala Glu Ala Tyr Gly His 500 505 510

WO 01/85772

PCT/GBO1/02003

atc Ile ggt atc cgc gtg gac Gly Ile Arg Val Asp 515 aag aag tet gat gtg gaa ggt gcg ttg ttg 1584 Lys Lys 520 Ser Asp Val Glu Gly Ala 525 Leu Leu gaa gca ttg aac caa aaa gac agg ctg gtg ttt atc gac ttc ctg acc 1632 Glu Ala Leu Asn Gin Lys Asp Arg Leu Val Phe Ile Asp Phe Leu Thr 530 535 540 gac cag aaa cag aat gtg atg ccc atg gtc ggc aac ggc aaa ggt ttg 1680 Asp Gin Lys Gin Asn Val Met Pro Met Val Gly Asn Gly Lys Gly Leu 545 550 555 560 gac gaa atg gta ctt ccg ccg cat atg cgt gcg gac gga aag gcg 1725 Asp Glu Met Val Leu Pro Pro His Met Arg Ala Asp Gly Lys Ala

565 570 575 <210> 30 <211> 575 <212> PRT

<213> Neisseria meningitidis <4GGx 30 Met Gin Leu Ser Gly Ala Gin Ile Ile Val Gin Ser Leu Lys Ala Glu 1 5 10 15 Gly Val Glu Tyr Val Phe Gly Tyr Pro Gly Gly Ala Val Ile Glu Ile 20 25 30 Tyr Asp Ala Leu Phe Gin Leu Asn Lys Phe Lys His Ile Leu Thr Arg 35 40 45 His Glu Gin Ala Ala Val His Ala Ala Asp Ala Tyr Ala Arg Val Ser 50 55 60 Gly Lys Val Gly Val Ala Leu Val Thr Ser Gly Pro Gly Val Thr Asn 65 70 75 80 Ala Leu Thr Gly Ile Ala Thr Ala Tyr Thr Asp Ser Ile Pro Met Val 85 90 95 Val Ile Ser Gly Gin Val Gly Asn Ser Leu Ile Gly Thr Asp Ala Phe 100 105 110 Gin Glu Val Asp Thr Val Gly Ile Thr Arg Pro Cys Val Lys His Asn 115 120 125 Phe Leu Val Thr Asp Ile Asn Glu Leu Ala Glu Thr Ile Lys Lys Ala

to ·

WO 01/85772 PCT/GBO1/02003 130 135 140 Phe Gin Ile Ala Ala Ser Gly Arg Pro Gly Pro Val Val Val Asp Val 145 150 155 160 Pro Lys Asp Val Thr Gin Ala Met Ala Lys Phe Ser Tyr Pro Gin Glu 165 170 175 Asp Ile Phe Ile Arg Ser Tyr Gin Pro Val Val Gin Gly His Ile Gly 180 185 190 Gin Ile Lys Lys Ala Val Gin Met Leu Ala Ser Ala Lys Arg Pro Val 195 200 205 Val Tyr Phe Gly Gly Gly Val Val Leu Gly Asn Ala Ser Glu Glu Leu 210 215 220 Thr Arg Phe Val Arg Met Thr Gly Ala Pro Cys Thr Gly Thr Leu Met 225 230 235 240 Gly Leu Gly Ala Tyr Pro Ser Gly Asp Arg Gin Phe Leu Gly Met Leu 245 250 OCR Gly Met His Gly Thr Tyr Glu Ala Asn Leu Ala Met Gin Asn Ala Asp 260 265 270 Val Val Leu Ala Val Gly Ala Arg Phe Asp Asp Arg Val Val Ser Val 275 280 285 Pro Ser Lys Phe Phe Glu Lys Ala Lys Lys Val Ile His Ile Asp Val 290 295 300 Asp Pro Ser Ser Ile Ala Lys Arg Val Lys Ala Asp Ile Pro Ile Val 305 310 315 320 Gly Asp Val Lys Asn Val Leu Ser Glu Met Val Ala Leu Trp Gin Lys 325 330 335 Gin Glu Ser Val Pro Ser Glu Asp Ala Leu Gly Lys Trp Trp Lys Thr 340 345 350 Ile Glu Glu Trp Arg Ser Arg Asp Cys Leu Trp Phe Asp Asn Gly Ser 355 360 365 Glu Ile Ile Lys Pro Gin Tyr Val Ile Gin Lys Leu Ala Glu Ile Thr 370 375 380 Gly Asn Ser Ala Ile Ile Thr Ser Asp Val Gly Gin His Gin Met Phe

WO 01/85772 PCT/GBO1/02003

385 390 395 400 Ala Ala Gin Tyr Tyr Pro Phe Glu Arg Pro Arg Gin Trp Leu Asn Ser 405 410 415 Gly Gly Leu Gly Thr Met Gly Val Gly Leu Pro Tyr Ala Ile Gly Ala 420 425 430 Lys Leu Ala Ala Pro Asp Gin Asp Val Phe Cys Ile Thr Gly Asp Gly 435 440 445 Ser Ile Gin Met Asn Ile Gin Glu Leu Ser Thr Cys Phe Gin Tyr Arg 450 455 460 Ile Pro Val Asn Val Ile Thr Leu Asn Asn Gly Tyr Leu Gly Met Val 465 470 475 480 Arg Gin Trp Gin Glu Ile Tyr Tyr Gly Gly Arg GlU Ser Glu Thr Tyr 485 490 495 Phe Asp Ser Leu Pro Asp Phe val Lys Leu ( Ala Glu Ala Tyr Gly His 500 505 510 Ile Gly Ile Arg Val Asp Lys Lys Ser Asp Val Glu Gly Ala Leu Leu 515 520 525 Glu Ala Leu Asn Gin Lys Asp Arg Leu Val Phe Ile Asp Phe Leu Thr 530 535 540 Asp Gin Lys Gin Asn Val Met Pro Met Val Gly Asn Gly Lys Gly Leu 545 550 555 560 Asp Glu Met Val Leu Pro Pro His Met Arg Ala Asp Gly Lys Ala

565 570 575 <210> 31 <211> 651 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1) .. (651) <400> 31 atg ttt tcc gta ccg cgt tcc ttt ttg ccg ggc gtt ttc gta ctt gcc 48 ·· 44

WO 01/85772 PCT/GB01/02003 Met Phe Ser Val Pro Arg Ser Phe Leu Pro Gly Val Phe Val Leu Ala 1 5 10 15 gcg ctt gcc gcc tgc aaa cct caa gac aac agt gcg gcg caa gtc gct 96 Ala Leu Ala Ala Cys Lys Pro Gin Asp Asn Ser Ala Ala Gin Val Ala 20 25 30 tet tca agt gca tcc gcg tcg gct gcg gaa aat gcg gca aag ccg caa 144 Ser Ser Ser Ala Ser Ala Ser Ala Ala Glu Asn Ala Ala Lys Pro Gin 35 40 45 acg cgc ggt acg gat atg cgt aag gaa gac atc ggc ggc gat ttc acg 192 Thr Arg Gly Thr Asp Met Arg Lys Glu Asp Ile Gly Gly Asp Phe Thr 50 55 60 ctg acc rra ggc gaa ggc vwc ttc aaC ctg age gat ttg aaa ggc 240 Leu Thr ,Asp Gly Glu Gly Lys Pro Phe Asn Leu Ser Asp Leu Lys Gly 65 70 75 80 aag gtc gtg att ctg tet ttc ggc ttt acg cac tgt ccc gat gtc tgc 288 Lys val Val Ile Leu Ser Phe Gly Phe Thr His Cys Pro Asp Val Cys 85 90 - 95 ccg aca gag ctt ttg acg tac age gac acg ttg aag cag ttg ggc ggg 336 Pro Thr Glu Leu Leu Thr Tyr Ser Asp Thr Leu Lys Gin Leu Gly Gly 100 105 110 cag gct aag gac gtg aaa gtg gtg ttc gtc age atc gat ccg gaa cgc 384 Gin Ala Lys Asp Val Lys val Val Phe Val Ser Ile Asp Pro Glu Arg 115 120 125 gac acg cct gaa atc atc ggc aag tat gcc aaa cag ttc aat ccg gac 432 Asp Thr Pro Glu Ile Ile Gly Lys Tyr Ala Lys Gin Phe Asn Pro Asp 130 135 140 ttt atc ggt ctg acg gca acg ggc gac caa aac ctg ccg gtc atc aag 480 Phe Ile Gly Leu Thr Ala Thr Gly Asp Gin Asn Leu Pro Val Ile Lys 145 150 155 160 cag caa tac cgc gtg gtt .tet gcc aaa gtc aat caa aaa gac gac age 528 Gin Gin Tyr Arg Val Val ser Ala Lys Val Asn Gin Lys Asp Asp Ser 165 170 175 gaa aac tat ttg gtc gac cac tet tcc ggt gcg tat ctc atc gac aaa 576 Glu Asn Tyr Leu Val Asp His Ser Ser Gly Ala Tyr Leu Ile Asp Lys 180 185 190 aac ggt gag gtt gcc att ttc tcg cct tac gga age gag ccg gaa acg 624

0· • 0 • 00 0 0 • 8 0 0 « • 0 • 0 0 0 00 0 0 0 • 0000 0 0 0 0 0 0 • 0 0 0 0 0*00 000« 0 0 000 00 0 0

WO 01/85772

Asn Gly Glu Val Ala Ile Phe Ser 200 195 att get gcc gat gta agg acc ctg Ile Ala Ala Asp Val Arg Thr Leu 210 215

PCT/GB01/02003

Pro Tyr Gly Ser Glu Pro Glu Thr 205 ctc 651

Leu <210> 32 <211> 217 <212> PRT <213> Neisseria meningitidis <400> 32

Met Phe Ser Val Pro Arg Ser Phe 1 5

Ala Leu Ala Ala Cys Lys Pro Gin 20

Ser Ser Ser Ala Ser Ala Ser Ala 35 40

Thr Arg Gly Thr Asp Met Arg Lys 50 55

Leu Thr Asp Gly Glu Gly Lys Pro 65 70

Lys Val Val Ile Leu Ser Phe Gly 85

Pro Thr Glu Leu Leu Thr Tyr Ser 100

Gin Ala Lys Asp Val Lys Val Val 115 120

Asp Thr Pro Glu Ile Ile Gly Lys 130 135

Phe Ile Gly Leu Thr Ala Thr Gly 145 150

Gin Gin Tyr Arg Val Val Ser Ala 165

Glu Asn Tyr Leu Val Asp His Ser

Leu Pro 10 Gly Val Phe Val Leu 15 Ala Asp 25 Asn Ser Ala Ala Gin 30 Val Ala Ala Glu Asn Ala Ala 45 Lys Pro Gin Glu Asp Iie Gly 60 Gly Asp Phe Thr Phe Asn Leu 75 Ser Asp Leu Lys Gly 80 Phe Thr 90 His Cys Pro Asp Val 95 Cys Asp 105 Thr Leu Lys Gin Leu 110 Gly Gly Phe Val Ser Ile Asp 125 Pro Glu Arg Tyr Ala Lys Gin 140 Phe Asn Pro Asp Asp Gin Asn 155 Leu Pro Val Ile Lys 160 Lys Val 170 Asn Gin Lys Asp Asp 175 Ser Ser Gly Ala Tyr Leu Ile Asp Lys

·· ·*··

180 185 190

WO 01/85772 • fc ·* • · « · fc' · fc 9 e β • •fcfc ···· ·· fc » 9 · fc

9 9 9

9 9 9

9 9»

9 9 99 9

9 9 · • · ·

9 9 9 • · · · »· fcfc

PCT/GB01/02003

Asn Gly Glu Val Ala Ile Phe Ser Pro Tyr Gly Ser Glu Pro Glu Thr 195 200 205 Ile Ala Ala Asp Val Arg Thr Leu Leu 210 215

<210> 33 <211> 330 <212> DNA <213> Neisseria meningitidis <22G>

<221> CDS <222> (1)..(330) <400> 33

ttg ccg ctg ctt tgt Cys 5 tgt ctt tgg ttt tgg cag cct gcg gcg gtg aaa Leu 1 Pro Leu Leu Cys Leu Trp Phe Trp 10 Gin Pro Ala Ala Val 15 Lys aag ccg ctg aag ctc ccg ctg ctg aag cac ctg ccg ccg aag ctc ccg Lys Pro Leu Lys Leu Pro Leu Leu Lys His Leu Pro Pro Lys Leu Pro 20 25 30 cta ctg aag cac ctg ccg ccg aag ctc ccg ctg ctg aag cac ctg ccg Leu Leu Lys His Leu Pro Pro Lys Leu Pro Leu Leu Lys His Leu Pro 35 40 45 ccg aag ctc ctg cta ctg aag cac ctg ccg ccg aag ctc ccg ctg ctg Pro Lys Leu Leu Leu Leu Lys His Leu Pro Pro Lys Leu Pro Leu Leu 50 55 60 aag ctg ccg cta ccg aag cac ctg ccg ctg aag ctg ccg cta ccg aag Lys Leu Pro Leu Pro Lys His Leu Pro Leu Lys Leu Pro Leu Pro Lys 65 70 75 80 cac ctg ccg ctg aag ctc ctg ctg ccg aag ctg caa aat aag cat ttt His Leu Pro Leu Lys Leu Leu Leu Pro Lys Leu Gin Asn Lys His Phe 85 90 95 ccg ctt gca aaa aag cag gat acg ttc agt atc ctg ctt ttt Pro Leu Ala Lys Lys Gin Asp Thr Phe Ser Ile Leu Leu Phe 100 105 110

144

192

240

288

330

·· ·· • · • • · 9 · · · • · · · • • • · • · • · • • • • · • • · ·'·'·· ···· • ·· • • • · · • · • · • • · • ·

WO 01/85772 PCT/GB01/02003 <210> 34 <211> 110 <212> PRT <213> Neisseria meningitidis <400> 34

Leu 1 Pro Leu Leu Cys 5 Cys Leu Trp Phe Trp 10 Gin Pro Ala Ala Val 15 Lys Lys Pro Leu Lys Leu Pro Leu Leu Lys His Leu Pro Pro Lys Leu Pro 20 25 30 Leu Leu Lys His Leu Pro Pro Lys Leu Pro Leu Leu Lys His Leu Pro 35 40 45 Pro Lys Leu Leu Leu Leu Lys His Leu Pro Pro Lys Leu Pro Leu Leu 50 55 60 Lys Leu Pro Leu Pro Lys His Leu Pro Leu Lys Leu Pro Leu Pro Lys 65 70 75 80 His Leu Pro Lsti T.xre — Τ.Λ1, Lsu XiSXl T ·«« ucu «» K7XAA •n — _ ZYOii Lys His Phe 85 90 95 Pro Leu Ala Lys Lys Gin Asp Thr Phe Ser Ile Leu Leu Phe

100 105 110 <210> 35 <211> 2118 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(2118) <400> 35

atg Met 1 ttc gac aaa cac gtt aag acc ttc caa tac ggt aat cag acc gtt Val 48 Phe Asp Lys His Val 5 Lys Thr Phe Gin Tyr 10 Gly Asn Gin Thr 15 act ttg gaa acc ggc gaa att gcc cgc caa gcc gcc get gcc gtt aaa 96 Thr Leu Glu Thr Gly Glu Ile Ala Arg Gin Ala Ala Ala Ala Val Lys 20 25 30

i

WO 01/85772 PCT/GB01/02003 gtc tet atg ggc gac acc gtg gtt ttg gtt gcc gtt acc acc aac aaa 144 Val Ser Met 35 Gly Asp Thr Val Val 40 Leu Val Ala Val Thr 45 Thr Asn Lys gaa gtg aaa gaa ggc caa gac ttc ttc ccc ctg acc gtc gat tat ttg 192 Glu Val 50 Lys Glu Gly Gin Asp 55 Phe Phe Pro Leu Thr 60 Val Asp Tyr Leu gaa cgc act tac gcc gca ggt aaa atc ccc ggc ggt ttc ttc aaa cgc 240 Glu 65 Arg Thr Tyr Ala Ala 70 Gly Lys Ile Pro Gly 75 Gly Phe Phe Lys Arg 80 gaa ggc aaa caa agc gaa aaa gaa atc ctg acc agc cgt ctg atc gac 288 Glu Gly Lys Gin Ser 85 Glu Lys Glu Ile Leu 90 Thr Ser Arg Leu Ile 95 Asp cgc ccg att cgt ccg ctg ttc ccc gaa ggt ttc tac cac gac atc caa 336 Arg Pro Ile Arg 100 Pro Leu Phe Pro Glu 105 Gly Phe Tyr His Asp 110 Ile Gin atc gta gcg atg gtc gtg tcc gtc gat ccc gaa atc gat tcc gac att 384 Ile Val Ala 115 Met Val Val Ser Val 120 Asp Pro Glu Ile Asp 125 Ser Asp Ile cct gca atg ttg ggc gcg tet gcc gcg ctg gtg ttg agc ggc gta ccg 432 Pro Ala 130 Met Leu Gly Ala Ser 135 Ala Ala Leu Val Leu 140 Ser Gly Val Pro ttt gcc ggt ccg att ggt gcg gca cgc gtg ggt tat ata aac ggc gtg 480 Phe 145 Ala Gly Pro Ile Gly 150 Ala Ala Arg Val Gly 155 Tyr Ile Asn Gly Val 160 tac gtt ttg aac ccg act aaa gcc gaa ttg gcg aaa tcg caa ttg gac 528 Tyr Val Leu Asn Pro 165 Thr Lys Ala Glu Leu 170 Ala Lys Ser Gin Leu 175 Asp ttg gtg gtc gcc ggt act tca aaa gcc gtg ttg atg gtg gaa tcc gaa 576 Leu Val Val Ala 180 Gly Thr Ser Lys Ala 185 Val Leu Met Val Glu 190 Ser Glu gcc aaa atc ctg ccc gaa gac gtg atg ctg ggc gcg gtg gtt tac ggc 624 Ala Lys Ile 195 Leu Pro Glu Asp Val 200 Met Leu Gly Ala Val 205 Val Tyr Gly cac gat caa atg cag gtt gcc atc aat gca atc aat gaa ttt gcc gac 672 His Asp 210 Gin Met Gin Val Ala 215 Ile Asn Ala Ile Asn 220 Glu Phe Ala Asp

WO 01/85772 PCT/GBO1/02003

gaa gtc aat ccg gaa ctt tgg gat tgg aaa gca cct gaa acc aat gag 720 Glu 225 Val Asn Pro Glu Leu 230 Trp Asp Trp Lys Ala 235 Pro Glu Thr Asn Glu 240 gaa ctg gtt gcc aaa gtc cgc ggg att gcc ggc gaa acc att aaa gaa 768 Glu Leu Val Ala Lys Val Arg Gly Ile Ala Gly Glu Thr Ile Lys Glu 245 250 255 gcg ttc aaa atc cgt caa aaa caa gcg cgt tet gcc aaa' ttg gac gaa 816 Ala Phe Lys Ile Arg Gin Lys Gin Ala Arg Ser Ala Lys Leu Asp Glu 260 265 270 get tgg agt gcg gta aaa gaa gcc ttg att acc gaa gaa acc gac acg 864 Ala Trp Ser Ala Val Lys Glu Ala Leu Ile Thr Glu Glu Thr Asp Thr 275 280 285 gca aSC atc aaa ggc att ttc aaa cac ttg gaa gcc gat 912 Leu Ala Ala Asn Glu Ile Lys Gly Ile Phe Lys His Leu Glu Ala Asp 290 295 300 gtc gtc cgc age caa att ttg gac ggc caa ccg cgc atc gac ggc cgc 960 Val Val Arg Ser Gin Ile Leu Asp Gly Gin Pro Arg Ile Asp Gly Arg 305 310 315 320 gac acc cgc acc gtc cgt ccg ctg aac atc caa acc ggc gta ttg ccg 1008 Asp Thr Arg Thr Val Arg Pro Leu Asn Ile Gin Thr Gly Val Leu Pro 325 330 335 cgc acg cac ggt tcc gca ttg ttt acc cgt ggc gaa acc caa gcc ctt 1056 Arg Thr His Gly Ser Ala Leu Phe Thr Arg Gly Glu Thr Gin Ala Leu 340 345 350 gcc gta gcg act ttg ggt act tcg cgc gac gag caa atc atc gac gcg 1104 Ala Val Ala Thr Leu Gly Thr Ser Arg Asp Glu Gin Ile Ile Asp Ala 355 360 365 ctg tcc ggc gaa tac acc gac cgc ttt atg ctg cac tac aac ttt ccg 1152 Leu Ser Gly Glu Tyr Thr Asp Arg Phe Met Leu His Tyr Asn Phe Pro 370 375 380 ccg tac tet acc ggc gaa gtg ggc cgc atg ggc gcg ccg aaa cgc cgt 1200 Pro Tyr Ser Thr Gly Glu val Gly Arg Met Gly Ala Pro Lys Arg Arg 385 390 395 400 gaa atc ggt cac ggt cgt ttg get aaa cgt gca ttg ttg gcc gta ttg 1248 Glu Ile Gly His Gly Arg Leu Ala Lys Arg Ala Leu Leu Ala Val Leu 405 410 415

WO 01/85772 PCT/GB01/02003 • ·

ccg aaa cct gaa gat Asp ttc Phe agc Ser tac acc atg cgc gtg gtc tcc gaa att 1296 Pro Lys Pro Glu 420 Tyr Thr 425 Met Arg Val Val Ser 430 Glu Ile acc gaa tcc aac ggc tet tcc tet atg get tcc gtc tgc ggc ggc tgc 1344 Thr GlU Ser Asn Gly Ser Ser Ser Met Ala Ser Val cys Gly Gly Cys 435 440 445 ttg agc ctg ctg tcc gcc ggc gtg cct ttg aaa gca cac gtt gcc ggt 1392 Leu Ser Leu Leu Ser Ala Gly Val Pro Leu Lys Ala His Val Ala Gly 450 455 460 atc gcg atg ggt ctg att ctg gaa ggc aat aag ttt gcc gtc ctg acc 1440 Ile Ala Met Gly Leu Ile Leu Glu Gly Asn Lys Phe Ala Val Leu Thr 465 470 475 480 gac att ttg ggc gac gaa gac cac ttg ggc gat atg gac ttt aaa gtg 1488 Asp Ile Leu Gly Asp Glu Asp His Leu Gly Asp Met Asp Phe Lys Val 485 490 495 get ggt acg acc gaa ggc gtt acc gcg ctg caa atg gac atc aaa atc 1536 Ala Gly Thr Thr Glu Gly Val Thr Ala Leu Gin Met Asp Ile Lys Ile 500 505 510 caa ggc att acc aaa gag att atg caa atc get ttg gcg cag gcc aaa 1584 Gin Gly Ile Thr Lys Glu Ile Met Gin Ile Ala Leu Ala Gin Ala Lys 515 520 525 gaa gcg cgt ctg cac atc ttg gat cag atg aaa gcc gcc gtt gcg ggc 1632 Glu Ala Arg Leu His Ile Leu Asp Gin Met Lys Ala Ala Val Ala Gly 530 535 540 ccg caa gag ctg tcc gca cac gcg cca cgc ttg ttc acg atg aaa atc 1680 Pro Gin Glu Leu ser Ala His Ala Pro Arg Leu Phe Thr Met Lys Ile 545 550 555 560 aac caa gac aaa atc cgc gaa gtt atc ggt aag ggc ggt gaa acc atc 1728 Asn Gin Asp Lys Ile Arg Glu Val Ile Gly Lys Gly Gly Glu Thr Ile 565 570 575 cgt gcg att acc get gaa acc ggt acg gaa atc aat att gcc gaa gac 1776 Arg Ala Ile Thr Ala Glu Thr Gly Thr Glu Ile Asn Ile Ala Glu Asp 580 585 590 ggt acg att acc att gcc gca acc act caa gaa gcc ggc gat gcg gcg 1824 Gly Thr Ile Thr Ile Ala Ala Thr Thr Gin Glu Ala Gly Asp Ala Ala 595 600 605

• · · ♦ · · • · · · · · · · · • · · · · · • · · · · ··· • · * · · · ···· ···· ·· ···

WO 01/85772 PCT/GB01/02003

aaa aaa cgc atc gag cag att act gcc gaa gtg gaa gtg ggc aaa gtg 1872 Lys Lys 610 Arg Ile Glu Gin Ile 615 Thr Ala Glu Val Glu 620 Val Gly Lys Val tac gaa ggc act gtg gtg aaa atc ctc gac aac aat gtc ggc gcg att 1920 Tyr Glu Gly Thr Val Val Lys Ile Leu Asp Asn Asn Val Gly Ala Ile 625 630 635 640 gtc agt gtg atg ccg ggc aaa gac ggt ttg gta cac atc agc caa atc 1968 Val Ser Val Met Pro Gly Lys Asp Gly Leu Val His Ile Ser Gin Ile 645 650 655 gcc cac gag cgc gta cgc aat gtc ggc gac tac ctg caa gtc ggt cag 2016 Ala His Glu Arg Val Arg Asn Val Gly Asp Tyr Leu Gin Val Gly Gin 660 665 670 gtg gtg aac gtg aaa gca ttg gaa gtg gac gac aga ggc cgt gtc cgt 2064 Val Val Asn Val Lys Ala Leu Glu -Val-Asp Asp Arg Gly Arg Val Arg 675 680 685 ctg tcc atc aaa gcc ctg ctg gac gcg cct gcc cgt gag gaa aat gcc 2112 Leu Ser Ile Lys Ala Leu Leu Asp Ala Pro Ala Arg GlU Glu Asn Ala 690 695 700 gcc gag 2118

Ala Glu 705 <210> 36 <211> 706 <212> PRT

<213> Neisseria meningitidis <400> 36 Met Phe Asp Lys His Val Lys Thr Phe Gin Tyr Gly Asn Gin Thr Val 1 5 10 15 Thr Leu Glu Thr Gly Glu Ile Ala Arg Gin Ala Ala Ala Ala Val Lys 20 25 30 Val Ser Met Gly Asp Thr Val Val Leu Val Ala Val Thr Thr Asn Lys 35 40 45 Glu Val Lys Glu Gly Gin Asp Phe Phe Pro Leu Thr Val Asp Tyr Leu 50 55 60 Glu Arg Thr Tyr Ala Ala Gly Lys Ile Pro Gly Gly Phe Phe Lys Arg

• fc fcfc • · · · fc · • · β fc • fcfcfc fcfcfcfc • fcfcfc fc

WO 01/85772

65 70 75

PCT/GB01/02003

Glu Gly Lys Gin Ser 85 Glu Lys GlU Ile Leu 90 Thr Ser Arg Leu Ile 95 Asp Arg Pro Ile Arg 100 Pro Leu Phe Pro Glu 105 Gly Phe Tyr His Asp 110 Ile Gin Ile Val Ala 115 Met Val Val Ser Val 120 Asp Pro Glu Ile Asp 125 Ser Asp Ile Pro Ala 130 Met Leu Gly Ala Ser 135 Ala Ala Leu Val Leu 140 Ser Gly Val Pro Phe 145 Ala Gly Pro Ile Gly 150 Ala Ala Arg Val Gly 155 Tyr Ile Asn Gly Val 160 Tyr Val Leu Asn Pro 165 Thr Lys Ala Glu Leu 170 Ala Lys Ser Gin Leu 175 Asp Leu Val Val Ala 180 Gly Thr Ser Lys Ala 185 Val Leu Met Val Glu 190 Ser Glu Ala Lys Ile 195 Leu Pro Glu Asp Val 200 Met Leu Gly Ala Val 205 Val Tyr Gly His Asp 210 Gin Met Gin Val Ala 215 Ile Asn Ala Ile Asn 220 Glu Phe Ala Asp Glu 225 Val Asn Pro Glu Leu 230 Trp Asp Trp Lys Ala 235 Pro Glu Thr Asn Glu 240 Glu Leu Val Ala Lys 245 Val Arg Gly Ile Ala 250 Gly Glu Thr Ile Lys 255 Glu Ala Phe Lys Ile 260 Arg Gin Lys Gin Ala 265 Arg Ser Ala Lys Leu 270 Asp Glu Ala Trp Ser 275 Ala Val Lys Glu Ala 280 Leu Ile Thr Glu Glu 285 Thr Asp Thr Leu Ala 290 Ala Asn Glu Ile Lys 295 Gly Ile Phe Lys His 300 Leu Glu Ala Asp Val 305 val Arg Ser Gin Ile 310 Leu Asp Gly Gin Pro 315 Arg Ile Asp Gly Arg 320 Asp Thr Arg Thr Val Arg Pro Leu Asn Ile Gin Thr Gly Val Leu Pro

···· ♦

WO 01/85772 PCT/GB01/02003 ·♦ ♦* • « · · • · « · · β · ♦ ··♦··«·

325 330 335

Arg Thr His Gly 340 Ser Ala Leu Phe Thr 345 Arg Gly Glu Thr Gin 350 Ala Leu Ala Val Ala 355 Thr Leu Gly Thr Ser 360 Arg Asp Glu Gin Ile 365 Ile Asp Ala Leu Ser 370 Gly Glu Tyr Thr Asp 375 Arg Phe Met Leu His 380 Tyr Asn Phe Pro Pro 385 Tyr Ser Thr Gly Glu 390 Val Gly Arg Met Gly 395 Ala Pro Lys Arg Arg 400 Glu Ile Gly His Gly 405 Arg Leu Ala Lys Arg 410 Ala Leu Leu Ala Val 415 Leu Pro Lys Pro Glu -Asp 420 Phe Ser Tyr Thr 425 Met Arg Val Val Ser 430 Glu Ile Thr Glu Ser 435 Asn Gly Ser Ser Ser 440 Met Ala Ser Val Cys 445 Gly Gly Cys Leu Ser 450 Leu Leu Ser Ala Gly 455 Val Pro Leu Lys Ala 460 His Val Ala Gly Ile 465 Ala Met Gly Leu Ile 470 Leu Glu Gly Asn Lys 475 Phe Ala Val Leu Thr 480 Asp Ile Leu Gly Asp 485 Glu Asp His Leu Gly 490 Asp Met Asp Phe Lys 495 Val Ala Gly Thr Thr 500 Glu Gly Val Thr Ala 505 Leu Gin Met Asp Ile 510 Lys Ile Gin Gly Ile 515 Thr Lys Glu Ile Met 520 Gin Ile Ala Leu Ala 525 Gin Ala Lys Glu Ala 530 Arg Leu His Ile Leu 535 Asp Gin Met Lys Ala 540 Ala Val Ala Gly Pro 545 Gin Glu Leu Ser Ala 550 His Ala Pro Arg Leu 555 Phe Thr Met Lys Ile 560 Asn Gin Asp Lys Ile 565 Arg Glu Val Ile Gly 570 Lys Gly Gly Glu Thr 575 Ile Arg Ala Ile Thr Ala Glu Thr Gly Thr Glu Ile Asn Ile Ala Glu Asp

• ·

WO 01/85772 PCT/GB01/02003 580 585 590 Gly Thr Ile Thr Ile Ala Ala Thr Thr Gin Glu Ala Gly Asp Ala Ala 595 600 605 Lys Lys Arg Ile Glu Gin Ile Thr Ala Glu Val Glu Val Gly Lys Val 610 615 620 Tyr Glu Gly Thr Val Val Lys Ile Leu Asp Asn Asn Val Gly Ala Ile 625 630 635 640 Val Ser val Met Pro Gly Lys Asp Gly Leu Val His Ile Ser Gin Ile 645 650 655 Ala His Glu Arg Val Arg Asn Val Gly Asp Tyr Leu Gin Val Gly Gin 660 665 670 Val Val- Asn Val Lys Ala Leu Glu Val Asp Asp Arg Gly Arg Val Arg 675 680 685 Leu Ser Ile Lys Ala Leu Leu Asp Ala Pro Ala Arg Glu Glu Asn Ala 690 695 700 - Ala Glu 705

<210> 37 <211> 3972 <212> DNA <213> Neisseria meningitidis <22 0>

<221> CDS <222> (1)..(3972) <400> 37 gtg age ega att atg tet gtc gtt ttg ccc ttg cgc ggc gtt acc gcc 48

Val Ser Arg Ile Met Ser-Val Val Leu Pro Leu Arg Gly Val Thr Ala

15 10 15 ctt tcc gat ttc cgt gtt gaa aaa ctc ttg caa aaa gcc gcc gca ctc 96

Leu Ser Asp Phe Arg Val Glu Lys Leu Leu Gin Lys Ala Ala Ala Leu

20 25 30 ggt ctg ccc gaa gtc aaa tta age age gaa ttt tgg tat ttc gtc ggc 144

Gly Leu Pro Glu Val Lys Leu Ser Ser Glu Phe Trp Tyr Phe Val Gly

WO 01/85772 PCT/GB01/02003 35 40 45 agc gag aaa gca ctt gat gcc gcg act gtc gaa aaa ctg caa gcc ttg 192 Ser Glu Lys Ala Leu Asp Ala Ala Thr Val Glu Lys Leu Gin Ala Leu 50 55 60 ttg gcg gcg caa agc gtt gaa cáa acg cca aaa gcg cgc gag ggc ttg 240 Leu Ala Ala Gin Ser Val Glu Gin Thr Pro Lys Ala Arg Glu Gly Leu 65 70 75 80 cat ttg ttt ttg gtc acg ccc cgt ttg ggt acg att tcg ccg tgg gct 288 His Leu Phe Leu Val Thr Pro Arg Leu Gly Thr Ile Ser Pro Trp Ala 85 90 95 tcc aag gcg acc aat atc gcg gaa aac tgc ggt ttg gca ggc atc gaa 336 Ser Lys Ala Thr Asn Ile Ala Glu Asn Cys Gly Leu Ala Gly Ile Glu 100 105 110 cgc atc gag cgc ggc atg gcg gtg tgg ctg gaa ggt gcg ctt acc gat 384 Arg Ile Glu Arg Gly Met Ala Val Trp Leu Glu Gly Ala Leu Thr Asp 115 120 125 gaa cag caa cag caa tgg gcg gct ttg ctg cac gac cgc atg act gaa . 432 Glu Gin Gin Gin Gin Trp Ala Ala Leu Leu His Asp Arg Met Thr Glu 130 135 140 tcc gtg ctg ccc gat ttt cag acg gca tcc aaa tta ttc cac cat ctc 480 Ser Val Leu Pro Asp Phe Gin Thr Ala Ser Lys Leu Phe His His Leu 145 150 155 160 gaa tcc gaa acc ttt tcc acc gtc gat gtt ttg ggc ggc ggt aaa gag 528 Glu Ser Glu Thr Phe Ser Thr Val Asp Val Leu Gly Gly Gly Lys Glu 165 170 175 gct ttg gtc aaa gcc aat acc gaa atg ggt ttg gca ctc tcc gcc gac 576 Ala Leu Val Lys Ala Asn Thr Glu Met Gly Leu Ala Leu Ser Ala Asp 180 185 190 gaa atc gat tat ctg gtt gaa aac tat cag gct ttg cag cgc aat ccg 624 Glu Ile Asp Tyr Leu Val Glu Asn Tyr Gin Ala Leu Gin Arg Asn Pro 195 200 205 tet gat gtg gag ctg atg atg ttc gcg cag gca aac agc gaa cac tgc 672 Ser Asp Val Glu Leu Met Met Phe Ala Gin Ala Asn Ser Glu His Cys 210 215 220 cgc cac aaa atc ttc aac gcc gac ttc atc ctc aac ggc gaa aaa cag 720 Arg His Lys Ile Phe Asn Ala Asp Phe Ile Leu Asn Gly Glu Lys Gin

WO 01/85772 PCT/GB01/02003 225 230 235 240

ccg aaa tcg ctc ttc ggt atg ata cgc gac acg cac aac gcg cat ccc Pro 768 Pro Lys Ser Leu Phe Gly 245 Met Ile Arg Asp 250 Thr His Asn Ala His 255 gaa ggc aca gtc gtt gcc tac aaa gac aac tea tcc gtg atc gaa ggc 816 Glu Gly Thr Val Val Ala Tyr Lys Asp Asn Ser Ser Val Ile Glu Gly 260 265 270 gca aaa gtc gaa cgt ttc tat cca aat gcg gca gaa aac caa ggc tac 864 Ala Lys Val Glu Arg Phe Tyr Pro Asn Ala Ala Glu Asn Gin Gly Tyr 275 280 285 cgt ttc cac gag gaa gac acg cat atc atc atg aaa gtg gaa acg cac 912 Arg Phe His Glu Glu Asp Thr His Ile Ile Met Lys Val Glu Thr His 290 295 300 aac cac ccg acc gcc atc gcg ccg ttt gcg ggc gcg gca acg ggc gcg 960 Asn His Pro Thr Ala Ile Ala Pro Phe Ala Gly Ala Ala Thr Gly Ala 305 310 315 320 ggc ggc ýdá atc Cgc gac gaa ggt gcg acg ggc aaa ggt tcg cgt ccg 1008 Gly Gly Glu Ile Arg Asp Glu Gly Ala Thr Gly Lys Gly Ser Arg Pro 325 330 335 aaa gca ggc ttg acc ggc ttt acc gtg tcc aac ctc aac atc ccc ggc 1056 Lys Ala Gly Leu Thr Gly Phe Thr Val Ser Asn Leu Asn Ile Pro Gly 340 345 350 ctc aaa cag ccg tgg gaa caa gat tac ggc aag ccg gaa cat att tcc 1104 Leu Lys Gin Pro Trp Glu Gin Asp Tyr Gly Lys Pro Glu His Ile Ser 355 360 365 tcg ccg ctg gac att atg att gaa ggc ccc atc ggc ggc gcg gcg ttc 1152 Ser Pro Leu Asp Ile Met Ile Glu Gly Pro Ile Gly Giy Ala Ala Phe 370 375 380 aac aac gaa ttc ggc cgc ccc aac ctc ttg ggc tac ttc cgc act ttt 1200 Asn Asn Glu Phe Gly Arg -Pro Asn Leu Leu Gly Tyr Phe Arg Thr Phe 385 390 395 400 gaa gaa aaa ttt gac ggt cag gtt cgc ggc tac cac aaa ccg att atg 1248 Glu Glu Lys Phe Asp Gly Gin Val Arg Gly Tyr His Lys Pro Ile Met 405 410 415 att gcc ggc ggc ttg ggc age att cag gcg cag cag acg cat aaa gac 1296 Ile Ala Gly Gly Leu Gly Ser Ile Gin Ala Gin Gin Thr His Lys Asp

*· ·· · · · · ·· ·

♦ · *· * · · · ·· · • · ·« • · · · · · • · · • · • « · : ι ·. • * ···· ···· • · · • · · · · '» · · · ·· ··

WO 01/85772 PCT/GB01/02003 420 425 430

gaa atc ccc gaa ggc gcg ttg ctg atc caa ctg ggc ggc ccg ggt atg 1344 Glu Ile Pro 435 Glu Gly Ala Leu Leu 440 Ile Gin Leu Gly Gly 445 Pro Gly Met ctt atc ggc ttg ggc ggc ggc gcg gct tet tcg atg gat acc ggc aca 1392 Leu Ile Gly Leu Gly Gly Gly Ala Ala Ser Ser Met Asp Thr Gly Thr 450 455 460 aac gac gcg tet tta gac ttc aac tcc gta caa cgc ggc aac ccc gaa 1440 Asn Asp Ala Ser Leu Asp Phe Asn Ser Val Gin Arg Gly Asn Pro Glu 465 470 475 480 atc gaa cgc cgc gcg cag gaa gtg atc gac cgc tgc tgg cag ctc ggc 1488 Ile Glu Arg Arg Ala Gin Glu Val Ile Asp Arg Cys Trp Gin Leu Gly 485 490 495 gac aaa aac ccg att atc tcc atc cac gac gtt ggc gcg ggc ggc ttg 1536 Asp Lys Asn Pro Ile Ile Ser Ile His Asp Val Gly Ala Gly Gly Leu 500 505 510 tcc aac gcc ttc ccc gaa ctc gtc aáe at ΛΛΓ. » — ~ rrrra '9 3 — r»rrr· ^— 3 — /*4“ a 33 1584 Ser Asn Ala Phe Pro Glu Leu Val Asn Asp Ala Gly Arg Gly Ala Val 515 520 525 ttc aag ctg cgc gaa gtg ccg ctg gaa gaa cac ggt ctc aac ccg ctg 1632 Phe Lys Leu Arg Glu Val Pro Leu Glu Glu His Gly Leu Asn Pro Leu 530 535 540 caa atc tgg tgc aac gaa tcg caa gag cgt tat gta ttg tcg att ttg 1680 Gin Ile Trp Cys Asn Glu Ser Gin Glu Arg Tyr Val Leu Ser Ile Leu 545 550 555 560 gaa aaa gat ttg gac atc ttc cgc tcc atc tgc gaa cgt gaa cgc tgc 1728 Glu Lys Asp Leu Asp Ile Phe Arg Ser Ile Cys Glu Arg Glu Arg Cys 565 570 575 ccg ttt gcg gta gtc ggt acg gcg acc gac gac ggc cat ttg aaa gta 1776 Pro Phe Ala Val Val Gly Thr Ala Thr Asp Asp Gly His Leu Lys Val 580 585 590 cgc gac gat ttg ttc tcc aac aac cct gtc gat ttg ccg ttg aac gtc 1824 Arg Asp Asp Leu Phe Ser Asn Asn Pro Val Asp Leu Pro Leu Asn Val 595 600 605 ttg ctc ggc aaa ccg ccc aaa acc acg cgt acc gac aaa acg gtt gcg 1872 Leu Leu Gly Lys Pro Pro Lys Thr Thr Arg Thr Asp Lys Thr Val Ala

WO 01/85772 PCT/GB01/02003 ·· ·· · ·· ···· ···· · · 9 4 4 4 9

4 4 4 9 9 · 9 · • 9 9 44 · · « 4 · • ' 9 9 4 9 ····

610 615 620 ccg tcc aaa aaa ccg ttt cac gcg ggc gat atc gac att act gaa gcc 1920 Pro Ser Lys Lys Pro Phe His Ala Gly Asp Ile Asp Ile Thr Glu Ala 625 630 635 640 gcc tac cgc gtt ctg cgc ctg cct gcc gta gcc gcc aaa aac ttc ctg 1968 Ala Tyr Arg Val Leu Arg Leu Pro Ala Val Ala Ala Lys Asn Phe Leu 645 650 655 att acc atc ggc gac cgc age gtc ggc ggc atg acc cac cgc gac caa 2016 Ile Thr Ile Gly Asp Arg Ser Val Gly Gly Met Thr His Arg Asp Gin 660 665 670 atg gtc ggc aaa tac caa acc ccc gta gcc gac tgc gcc gtt acc atg 2064 Met Val Gly Lys Tyr Gin Thr Pro Val Ala Asp Cys Ala Val Thr Met 675 680 685 atg ggc ttc aac act tat cgc ggc gaa gcg atg age atg ggc gaa aaa 2112 Met Gly Phe Asn Thr Tyr Arg Gly Glu Ala Met Ser Met Gly Glu Lys 690 695 700 ccg acc gtc gcc ctg ttt gac gcg cct gct tcg ggc aga atg tgc gtc 2160 Pro Thr Val Ala Leu Phe Asp Ala Pro Ala Ser Gly Arg Met Cys Val 705 710 715 720 ggc gaa gcc atc acc aac atc gcg gca gcc aat atc ggc gac atc ggc 2208 Gly Glu Ala Ile Thr Asn Ile Ala Ala Ala Asn Ile Gly Asp Ile Gly 725 730 735 aac atc aaa ctc tcc gcc aac tgg atg gcg gcg tgc ggc aac gaa ggc 2256 Asn Ile Lys Leu Ser Ala Asn Trp Met Ala Ala Cys Gly Asn Glu Gly 740 745 750 gaa gac gaa aaa ctc tac cgc acc gtc gaa gcg gtt tcc aaa gcc tgt 2304 Glu Asp Glu Lys Leu Tyr Arg Thr Val Glu Ala Val Ser Lys Ala Cys 755 760 765 cag gca ttg gat ttg age att ccc gtg ggc aaa gac age ctg tcg atg 2352 Gin Ala Leu Asp Leu Ser -Ile Pro Val Gly Lys Asp Ser Leu Ser Met 770 775 780 aaa acc gtg tgg cag gac ggc gaa gag aaa aaa tcc gtc gtt tcg ccg 2400 Lys Thr Val Trp Gin Asp Gly Glu Glu Lys Lys Ser Val Val Ser Pro 785 790 795 800 ttg age ctg att atc tcc gcg ttc gcg ccg gtt caa gac gta cgc aaa 2448 Leu Ser Leu Ile Ile Ser Ala Phe Ala Pro Val Gin Asp Val Arg Lys

WO 01/85772 PCT/GB01/02003 • to · to · · to · toto · • · · to to · · · • · · · » · · to · · • · ··· · · · · ···· ···· ·· ··· ·· ··

805 810 815 acc gtt acg ccc gaa ttg aaa aac gtc gag gac agc gta ttg ctg ttt 2496 Thr Val Thr Pro Glu Leu Lys Asn val Glu Asp ser Val Leu Leu Phe 820 825 830 gtc gat ttg ggc ttc ggc aaa gcg cgt atg ggc ggc tcg gcg ttt ggt 2544 Val Asp Leu Gly Phe Gly Lys Ala Arg Met Gly Gly Ser Ala Phe Gly 835 840 845 cag gtg tac aac aat atg agc ggc gac gcg ccc gat ttg gac gat aca 2592 Gin Val Tyr Asn Asn Met Ser Gly Asp Ala Pro Asp Leu Asp Asp Thr 850 855 860 agt cgt ctg aaa gcg ttt tac aac gtg att cag cag ctt gtc gcc gaa 2640 Ser Arg Leu Lys Ala Phe Tyr Asn Val Ile Gin Gin Leu Val Ala Glu 865 870 875 880 gac aaa ctc ttg gcg tat cac gac cgc agc gac ggc ggc ttg ttt gcc 2688 Asp Lys Leu Leu Ala Tyr His Asp Arg Ser Asp Gly Gly Leu Phe Ala 885 890 895 gtt ttg gta gaa atg gcg ttt gca ggg cgg tgc ggc ttg gat aha fr»4· 9 — —¹ 273£ Val Leu Val Glu Met Ala Phe Ala Gly Arg Cys Gly Leu Asp Ile Asp 900 905 910 tta aat tta ttg ctt gca caa aca ttt att acc aac cat acc gct ctg 2784 Leu Asn Leu Leu Leu Ala Gin Thr Phe Ile Thr Asn His Thr Ala Leu 915 920 925 tet caa tea ttg cgg act gaa gag gta aaa gcg ttg gct gaa tgg caa 2832 Ser Gin Ser Leu Arg Thr Glu Glu Val Lys Ala Leu Ala Glu Trp Gin 930 935 940 gaa acc att gcc cgc aca tta ttt aat gaa gag ttg ggt gct gtt atc 2880 Glu Thr Ile Ala Arg Thr Leu Phe Asn Glu Glu Leu Gly Ala Val Ile 945 950 955 960 caa gtt aga aaa caa gat gtt gcc gat att atc aat tta ttc tat caa 2928 Gin Val Arg Lys Gin Asp Val Ala Asp Ile Ile Asn Leu Phe Tyr Gin 965 970 975 caa cag ctg cat cat aat gtc ttt gaa atc ggt acg tta act gat gag 2976 Gin Gin Leu His His Asn Val Phe Glu Ile Gly Thr Leu Thr Asp Glu 980 985 990 aac acg tta atc atc cgc gac ggg caa acg cac ctt att tet gac aac 3024 Asn Thr Leu Ile Ile Arg Asp Gly Gin Thr His Leu Ile Ser Asp Asn

• « 0 00 0

WO 01/85772 PCT/GBO1/02003

0« 00 00 0 ♦ 000 000 0 · · 0

0 0 000 0000

995 1000 1005

cta atc aaa Leu Ile Lys 1010 ctg Leu caa caa acc Gin Gin Thr 1015 tgg caa gaa Trp Gin Glu acc agc cat caa atc caa 3072 Thr Ser 1020 His Gin Ile Gin cgc ctg cgc gac aac cct gcc tgc gcc gac agc gag ttt gcc ctg att 3120 Arg Leu Arg Asp Asn Pro Ala Cys Ala Asp Ser Glu Phe Ala Leu Ile 1025 1030 1035 1040 ggc gac aac gga cgc agc gca ttg ttt gcc aac ctg aaa ttc gac gtg 3168 Gly Asp Asn Gly Arg Ser Ala Leu Phe Ala Asn Leu Lys Phe Asp Val 1045 1050 1055 aac gaa gac atc gcc gcg ccg ttt atc aac agc ggc gcg aaa ccc aaa 3216 Asn Glu Asp Ile Ala Ala Pro Phe ^zXle Asn Ser Gly Ala Lys Pro Lys 1060 1065 1070 atc gcc atc ctg cgc gaa cag ggc gta aac ggg caa atc gaa atg gcc 3264 Ile Ala Ile Leu Arg Glu Gin Gly Val Asn Gly Gin Ile Glu Met Ala 1075 1080 1085 gcc gcc ttc acc cgt gcc ggt ttc gat gqc tac gac gtg cat atg tcc 3312 Ala Ala Phe Thr Arg Ala Gly Phe Asp Ala Tyr Asp Val His Met Ser 1090 1095 1100 gac ctg atg gca ggc cgc gtc cac ctt gcc gac ttc aaa atg ctg gcg 3360 Asp Leu Met Ala Gly Arg Val His Leu Ala Asp Phe Lys Met Leu Ala 1105 1110 1115 1120 gcg tgc ggc ggc ttc agc tac ggc gac gta ctc ggc gcg ggc aaa ggc 3408 Ala Cys Gly Gly Phe Ser Tyr Gly Asp Val Leu Gly Ala Gly Lys Gly 1125 1130 1135 tgg gcg aaa tcc atc ctg ttc cac ccc gcc tta cgc gac cag ttc gcc 3456 Trp Ala Lys Ser Ile Leu Phe His Pro Ala Leu Arg Asp Gin Phe Ala 1140 1145 1150 gcc ttc ttc acc gac ccg aac acg ctg aca ttg ggc gtg tgc aac ggc 3504 Ala Phe Phe Thr Asp Pro -Asn Thr Leu Thr Leu Gly Val Cys Asn Gly 1155 1160 1165 tgc cag atg gtc agc aac ctc gcc gaa atc atc ccc ggc acg gca ggc 3552 Cys Gin Met Val Ser Asn Leu Ala Glu Ile Ile Pro Gly Thr Ala Gly 1170 1175 1180 tgg ccg aag ttc aag cgc aac ctg agc gaa cag ttc gaa gcg cgc ctg 3600 Trp Pro Lys Phe Lys Arg Asn Leu Ser Glu Gin Phe Glu Ala Arg Leu

····

WO 01/85772 PCT/GBO1/02003

1185 1190 1195 1200 age atg gtt cac gtc ccc aaa tea gcc tcc ctg att ctg aac gaa atg 3648 Ser Met Val His Val Pro Lys Ser Ala Ser Leu Ile Leu Asn Glu Met 1205 1210 1215 caa ggc tcc age ctg ccc gtc gtc gtc age cac ggc gaa ggc ege gcc 3696 Gin Gly Ser Ser Leu Pro Val Val Val Ser His Gly Glu Gly Arg Ala 1220 1225 1230 gac ttc gcg ctt cac ggc ggc aac att tet gcc gat ttg ggc att gcg 3744 Asp Phe Ala Leu His Gly Gly Asn Ile Ser Ala Asp Leu Gly Ile Ala 1235 1240 1245 ttg caa tat gtg gac gga caa aac caa att acc caa acc tac ccg ctc 3792 Leu Gin Tyr Val Asp Gly Gin Asn Gin Ile Thr Gin Thr Tyr Pro Leu 1250 1255 1260 aac ccc aac ggc tcg ccg caa ggc atc gcc ggc gtg acc aac gcc gac 3840 Asn Pro Asn Gly Ser Pro Gin Gly Ile Ala Gly Val Thr Asn Ala Asp 1265 1270 1275 1280 ggc ege gtt acc atc atg atg ccg /'/'I· waa «/*+· <»♦·» ---- SJVU WAV, ege gcc 3888 Gly Arg Val Thr Xle Met Met Pro His Pro Glu Arg Val Tyr Arg Ala 1285 1290 L295 gca caa atg age tgg aaa ccg gaa gac tgg acg gaa ttg tcc ggc tgg 3936 Ala Gin Met Ser Trp Lys Pro Glu Asp Trp Thr Glu Leu Ser Gly Trp 1300 1305 1310 tac ege ctc ttc gcc ggc gca cgt aaa gcc ttg ggc 3972 Tyr Arg Leu Phe Ala Gly Ala Arg Lys Ala Leu Gly

1315 1320 <210> 38 <211> 1324 <212> PRT <213> Neisseria meningitidis <400> 38

Val Ser Arg Ile Met Ser Val Val Leu Pro Leu Arg Gly Val Thr Ala 1 5 10 15 Leu Ser Asp Phe Arg Val Glu Lys Leu Leu Gin Lys Ala Ala Ala Leu 20 25 30 Gly Leu Pro Glu Val Lys Leu Ser Ser Glu Phe Trp Tyr Phe Val Gly

• fl • · • fl ···· • · • · « fl ··«« • fl fl • · fl · fl * · • · · • · fl ·· WO 01/85772 35 40 45 PCT/GB01/02003 Ser Glu Lys Ala Leu Asp Ala Ala Thr Val 50 55 Glu Lys Leu Gin 60 Ala Leu Leu Ala Ala Gin Ser Val Glu Gin Thr Pro 65 70 Lys Ala Arg Glu 75 Gly Leu 80 His Leu Phe Leu Val Thr Pro Arg Leu Gly 85 90 Thr Ile Ser Pro Trp 95 Ala Ser Lys Ala Thr Asn Ile Ala Glu Asn Cys 100 105 Gly Leu Ala Gly 110 Ile Glu Arg Ile Glu Arg Gly Met Ala Val Trp Leu 115 120 Glu Gly Ala Leu 125 Thr Asp Glu Gin Gin Gin Gin Trp Ala Ala Leu Leu 130 135 His Asp Arg Met 140 Thr Glu Ser Val Leu Pro Asp Phe Gin Thr Ala Ser 145 150 Lys Leu Phe His 155 His Leu 160 Glu Ser Glu Thr Phe Ser Thr Val Asp Val 165 170 Leu Gly Gly Gly Lys 175 Glu Ala Leu Val Lys Ala Asn Thr Glu Met Gly 180 185 Leu Ala Leu Ser 190 Ala Asp Glu Ile Asp Tyr Leu Val Glu Asn Tyr Glii 195 200 Ala Leu Gin Arg 205 Asn Pro Ser Asp Val Glu Leu Met Met Phe Ala Gin 210 215 Ala Asn Ser Glu 220 His Cys Arg His Lys Ile Phe Asn Ala Asp Phe Ile 225 230 Leu Asn Gly Glu 235 Lys Gin 240 Pro Lys Ser Leu Phe Gly Met Ile Arg Asp 245 - 250 Thr His Asn Ala His 255 Pro Glu Gly Thr Val Val Ala Tyr Lys Asp Asn 260 265 Ser Ser Val Ile 270 Glu Gly Ala Lys Val Glu Arg Phe Tyr Pro Asn Ala 275 280 Ala Glu Asn Gin 285 Gly Tyr Arg Phe His Glu Glu Asp Thr His Ile Ile 78 Met Lys Val Glu Thr His

fcfc ·· fcfc · · fcfc fcfc • · · · fcfcfcfc · · • fc ♦ fc α fc · fc

WO 01/85772 PCT/GB01/02003

290 295 300

Asn 305 His Pro Thr Ala Ile .310 Ala Pro Phe Ala Gly 315 Ala Ala Thr Gly Ala 320 Gly Gly Glu Ile Arg 325 Asp Glu Gly Ala Thr 330 Gly Lys Gly Ser Arg 335 Pro Lys Ala Gly Leu 340 Thr Gly Phe Thr Val 345 ser Asn Leu Asn Ile 350 Pro Gly Leu Lys Gin 355 Pro Trp Glu Gin Asp 360 Tyr Gly Lys Pro Glu 365 His Ile Ser Ser Pro 370 Leu Asp Ile Met Ile 375 Glu Gly Pro Ile Gly 380 Gly Ala Ala Phe Asn 385 Asn Glu Phe Gly Arg 390 Pro Asn Leu Leu Gly 395 Tyr Phe Arg Thr Phe 400 Glu Glu Lys Phe Asp 405 Gly Gin Val Arg Gly 410 Tyr His Lys Pro Ile 415 Met Ile Ala Gly Gly 420 Leu Gly Ser Ile Gin 425 Ala Gin Gin Thr His 430 Lys Asp Glu Ile Pro 435 Glu Gly Ala Leu Leu 440 Ile Gin Leu Gly Gly 445 Pro Gly Met Leu Ile 450 Gly Leu Gly Gly Gly 455 Ala Ala Ser Ser Met 460 Asp Thr Gly Thr Asn 465 Asp Ala Ser Leu Asp 470 Phe Asn Ser Val Gin 475 Arg Gly Asn Pro Glu 480 Ile Glu Arg Arg Ala 485 Gin Glu Val Ile Asp Arg 490 Cys Trp Gin Leu 495 Gly Asp Lys Asn Pro 500 Ile Ile Set Ile His 505 Asp Val Gly Ala Gly 510 Gly Leu Ser Asn Ala 515 Phe Pro Glu Leu Val 520 Asn Asp Ala Gly Arg 525 Gly Ala Val Phe Lys 530 Leu Arg Glu Val Pro 535 Leu Glu Glu His Gly 540 Leu Asn Pro Leu Gin Ile Trp Cys Asn Glu Ser Gin Glu Arg Tyr Val Leu Ser Ile Leu

·· • · 9 9 9 9 9 9 99 · 9 9 9 9 9 99 9 9 9 • 9 9 9 99 • 9 9 9 9 9 9 • 9 9 9 9 9 99 9 9999 9 9 ··· 99 9 ·

WO 01/85772 PCT/GB01/02003 545 550 555 560 Glu Lys Asp Leu Asp Ile Phe Arg Ser Ile Cys Glu Arg Glu Arg Cys 565 570 575 Pro Phe Ala Val Val Gly Thr Ala Thr Asp Asp Gly His Leu Lys Val 580 585 590 Arg Asp Asp Leu Phe Ser Asn Asn Pro Val Asp Leu Pro Leu Asn Val 595 600 605 Leu Leu Gly Lys Pro Pro Lys Thr Thr Arg Thr Asp Lys Thr Val Ala 610 615 620 Pro Ser Lys Lys Pro Phe His Ala Gly Asp Ile Asp Ile Thr Glu Ala 625 630 635 640 Ala Tyr Arg Val Leu Arg Leu Pro Ala Val Ala Ala Lys Asn Phe Leu 645 650 655 Ile Thr Ile Gly Asp Arg Ser Val Gly Gly Met Thr His Arg Asp Gin 660 665 670 Met Val Gly Lys Tyr Gin Thr Pro Val Ala Asp Cys Ala Val Thr Met 675 680 685 Met Gly Phe Asn Thr Tyr Arg Gly Glu Ala Met Ser Met Gly Glu Lys 690 695 700 Pro Thr Val Ala Leu Phe Asp Ala Pro Ala Ser Gly Arg Met Cys Val 705 710 715 720 Gly Glu Ala Ile Thr Asn Ile Ala Ala Ala Asn ile Gly Asp Ile Gly 725 730 735 Asn Ile Lys Leu Ser Ala Asn Trp Met Ala Ala Cys Gly Asn Glu Gly 740 745 750 Glu Asp Glu Lys Leu Tyr Arg Thr Val Glu Ala Val Ser Lys Ala Cys 755 . 760 765 Gin Ala Leu Asp Leu Ser Ile Pro Val Gly Lys Asp Ser Leu Ser Met 770 775 780 Lys Thr Val Trp Gin Asp Gly Glu Glu Lys Lys Ser Val Val Ser Pro 785 790 795 800 Leu Ser Leu Ile Ile Ser Ala Phe.Ala Pro Val Gin Asp Val Arg Lys

eo

WO 01/85772 PCT/GB01/02003

• 9 • • 9 • · · 9 9 · 9 9 99 9 9 9 99 9 9 9 9 9 • 9 99 9 • o • 9 9 9 • O 9 9 9 ··· • 9999 99 999 99 99

805 810 815 Thr Val Thr Pro Glu Leu Lys Asn Val 820 825 Glu Asp Ser Val Leu Leu Phe 830 Val Asp Leu 835 Gly Phe Gly Lys Ala Arg 840 Met Gly Gly Ser 845 Ala Phe Gly Gin Val Tyr 850 Asn Asn Met Ser Gly Asp 855 Ala Pro Asp Leu 860 Asp Asp Thr Ser Arg Leu 865 Lys Ala Phe Tyr Asn Val 870 Ile Gin Gin Leu 875 Val Ala Glu 880 Asp Lys Leu Leu Ala Tyr His Asp Arg 885 Ser Asp Gly Gly 890 Leu Phe Ala 895 Val Leu Val Glu Met Ala Phe Ala Gly 900 905 Arg Cys Gly Leu Asp Ile Asp 910 Leu Asn Leu 915 Leu Leu Ala Gin Thr Phe 920 Ile Thr Asn His 925 Thr Ala Leu Ser Gin Ser 930 Leu Arg Thr Glu Glu Val 935 Lys Ala Leu Ala 940 Glu Trp Gin Glu Thr Ile 945 Ala Arg Thr Leu Phe Asn 95.0 Glu Glu Leu Gly 955 Ala Val Ile 960 Gin Val Arg Lys Gin Asp Val Ala Asp 965 Ile Ile Asn Leu 970 Phe Tyr Gin 975 Gin Gin Leu His His Asn Val Phe Glu 980 985 ile Gly Thr Leu Thr Asp Glu 990 Asn Thr Leu 995 Ile Ile Arg Asp Gly Gin 1000 Thr His Leu Ile 1005 Ser Asp Asn Leu Ile Lys 1010 Leu Gin Gin Thr Trp Gin 1015 Glu Thr Ser His 1020 Gin Ile Gin Arg Leu Arg Asp Asn Pro Ala Cys Ala 025 1030 Asp Ser Glu Phe 1035 Ala Leu Ile 1040 Gly Asp Asn Gly Arg Ser Ala Leu Phe Ala Asn Leu Lys 1045 1050 Phe Asp Val 1055 Asn Glu Asp Ile Ala Ala Pro Phe Ile Asn Ser Gly Ala Lys Pro Lys

·· φφ · ·· ···· • φ · ·· · φ · • β φ φ φ ΦΦΦ φ · φ φ · φφφφ φ φ · φ φ φφφφ φφ φφφφ φφ ΦΦΦ φφ φφ

W0 01/85772 PCT/GB01/02003

1060 1065 1070

Xle Ala Ile 1075 Leu Arg Glu Gin Gly Val Asn Gly Gin Ile Glu Met Ala 1080 1085 Ala Ala Phe Thr Arg Ala Gly Phe Asp Ala Tyr Asp Val His Met Ser 1090 1095 1100 Asp Leu Met Ala Gly Arg Val His Leu Ala Asp Phe Lys Met Leu Ala 105 1110 1115 1120 Ala Cys Gly Gly Phe Ser Tyr Gly Asp Val Leu Gly Ala Gly Lys Gly 1125 1130 1135 Trp Ala Lys Ser Ile Leu Phe His Pro Ala Leu Arg Asp Gin Phe Ala 1140 . 1145 1150 Ala Phe Phe Thr Asp Pro Asn Thr Leu Thr Leu Gly Val Cys Asn Gly 1155 1160 1165 Cys Gin Met Val Ser Asn Leu Ala Glu Ile Ile Pro Gly Thr Ala Gly 1170 1175 1180 Trp Pro Lys Phe Lys Arg Asn Leu Ser Glu Gin Phe Glu Ala Arg Leu 185 1190 1.195 1200 Ser Met Val His Val Pro Lys Ser Ala Ser Leu Ile Leu Asn Glu Met 1205 1210 1215 Gin Gly Ser Ser Leu Pro Val Val Val Ser His Gly Glu Gly Arg Ala 1220 1225 1230 Asp Phe Ala Leu His Gly Gly Asn Ile Ser Ala Asp Leu Gly Ile Ala 1235 1240 1245 Leu Gin Tyr Val Asp Gly Gin Asn Gin Ile Thr Gin Thr Tyr Pro Leu 1250 1255 1260 Asn Pro Asn Gly Ser Pro Gin Gly Ile Ala Gly Val Thr Asn Ala Asp 265 1270 , 1275 1280 Gly Arg Val Thr Ile Met Met Pro His Pro Glu Arg Val Tyr Arg Ala 1285 1290 1295 Ala Gin Met Ser Trp Lys Pro Glu Asp Trp Thr Glu Leu Ser Gly Trp

1300 1305 1310

Tyr Arg Leu Phe Ala Gly Ala Arg Lys Ala Leu Gly

WO 01/85772

1315 1320

PCT/GB01/02003 <210> 39 <211> 471 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(471) <400> 39

atg Met 1 tcc cgc aat acc gaa gag ctg caa ggc atc tcg ctt ttg ggt aat 48 Ser Arg Asn Thr Glu 5 Glu Leu Gin Gly 10 Ile Ser Leu Leu Gly 15 Asn cag aaa acc ega tat ccg acc ggc tac gcg cct gaa att ctc gaa gca 96 Gin Lys Thr Arg Tyr Pro Thr Gly Tyr Ala Pro Glu Ile Leu Glu Ala 20 25 30 ttc gac aac aaa cat ccc gac aac gac tat ttc gtc aaa ttc gtc tgc 144 Phe Asp Asn Lys His Pro Asp Asn Asp Tyr Phe Val Lys Phe Val Cys 35 40 45 cca gag ttc acc agc ctc tgc ccc atg acc ggg cag ccc gac ttc gcc 192 Pro Glu Phe Thr Ser Leu Cys Pro Met Thr Gly-Gin Pro Asp 'Phe Ala 50 55 60 acc atc tac atc cgc tac att ccg cac atc aaa atg gtg gaa agc aaa 240 Thr Ile Tyr Ile Arg Tyr Ile Pro His Ile Lys Met Val Glu Ser Lys 65 70 75 80 tcc ctg aaa ctc tac ctc ttc agc ttc cgc aac cac ggc gat ttt cat 288 Ser Leu Lys Leu Tyr Leu Phe Ser Phe Arg Asn His Gly Asp Phe His 85 . 90 95 gaa gac tgc gtc aac atc atc atg aaa gac ctc att gcc ctg atg gat 336 Glu Asp Cys Val Asn Ile Ile Met Lys Asp Leu Ile Ala Leu Met Asp 100 105 110 ccg aaa tac atc gaa gta ttc ggc gag ttc aca ccg cgc ggc ggc atc 384 Pro Lys Tyr Ile Glu Val Phe Gly Glu Phe Thr Pro Arg Gly Gly Ile 115 120 125 gcc att cat cct ttc gcc aat tac ggc aaa gca ggc acc gag ttt gaa 432 Ala Ile His Pro Phe Ala Asn Tyr Gly Lys Ala Gly Thr GlU Phe Glu

44 4444

WO 01/85772 PCT/GB01/02003

130 135 140 gca ttg gcg cgt aaa cgc ctg ttt gag cac gac gca caa 471 Ala Leu Ala Arg Lys Arg Leu Phe Glu His Asp Ala Gin 145 150 155

<210> 40 <211> 157 <212> PRT <213> Neisseria meningitidis <400> 40

Met Ser Arg Asn Thr Glu Glu Leu Gin Gly Ile Ser Leu Leu Gly 15 Asn 1 5 10 Gin Lys Thr Arg Tyr Pro Thr Gly Tyr Ala Pro Glu Ile Leu Glu Ala 20 25 30 Phe Asp Asn Lys His Pro Asp Asn Asp Tyr Phe Val Lys Phe Val Cys 35 40 45 pro Glu Phe Thr Ser Leu Cys Pro Met Thr Gly Gin Pro Asp Phe Ala 50 55 60 Thr Ile Tyr Ile Arg Tyr Ile Pro His Ile Lys Met Val Glu Ser Lys 65 70 75 80 Ser Leu Lys Leu Tyr Leu Phe Ser Phe Arg Asn His Gly Asp Phe His 85 90 95 Glu Asp Cys Val Asn Ile Ile Met Lys Asp Leu Ile Ala Leu Met Asp 100 105 110 Pro Lys Tyr Ile Glu val Phe Gly Glu Phe Thr Pro Arg Gly Gly Ile 115 120 125 Ala Ile His Pro Phe Ala Asn Tyr Gly Lys Ala Gly Thr Glu Phe Glu 130 135 140 Ala Leu Ala Arg Lys Arg Leu Phe Glu His Asp Ala Gin

145 150 155 <210> 41 <211> 504 <212> DNA ·· ····

WO 01/85772 PCT/GBO1/02003 <213> Neisseria meningitidis ₄ <220>

<221> CDS <222> (1)..(504) <400> 41

atg ccc cta Leu cta Leu gac Asp 5 agt ttc Ser Phe aaa Lys gtc gat cac acc cgt atg cat Met His 15 gcc Ala 48 Met 1 Pro Val Asp 10 His Thr Arg ccc gcc gta cgc gtg gcg aaa acc atg acc acg ccc aaa ggc gac acc 96 Pro Ala Val Arg Val Ala Lys Thr Met Thr Thr Pro Lys Gly Asp Thr 20 25 30 att acc gtg ttc gac ctg cgc ttt tgc gtt ccc aat aaa gaa atc ctg 144 Ile Thr Val Phe Asp Leu Arg Phe Cys Val Pro Asn Lys Glu Ile Leu 35 40 45 cct gaa aaa ggc ata cac acg ctg gag cat ttg ttc gca ggt ttt atg 192 Pro Glu Lys Gly Ile His Thr Leu Glu His Leu Phe Ala Gly Phe Met 50 55 60 cgc gac cac t.trr --9 aac γτγτγ· 9 -a — SSLC ΓΤΓΤΖ* gaa atc α y αν· — ^4CL L. U tcc CCy 240 Arg Asp His Leu Asn Gly Asn Gly Val Glu Ile Ile Asp Ile Ser Pro 65 70 75 80 atg ggc tgc cgc acc ggt ttt tat atg agc ctt atc ggc acg cct tcc 288 Met Gly Cys Arg Thr Gly Phe Tyr Met Ser Leu Ile Gly Thr Pro Ser 85 90 95 gaa cag cag gtc gcc gat gcg tgg ctg gct tcg atg cag gat gtt ttg 336 Glu Gin Gin Val Ala Asp Ala Trp Leu Ala Ser Met Gin Asp Val Leu 100 105 110 aat gtc aaa gac caa agc aaa atc ccc gag ttg aac gaa tac caa tgc 384 Asn Val Lys Asp Gin Ser Lys Ile Pro Glu Leu Asn Glu Tyr Gin Cys 115 120 125 ggc act tat caa atg cac tcg ctc gcc gaa gcg cag caa atc gcg caa 432 Gly Thr Tyr Gin Met His ' Ser Leu Ala Glu Ala Gin Gin Ile Ala Gin 130 135 140 aac gtg ttg gcg cgc aaa gtg gcg gtg aac aaa aat gaa gag ctg acg 480 Asn Val Leu Ala Arg Lys Val Ala Val Asn Lys Asn Glu Glu Leu Thr 145 150 155 160 ctg gat gaa ggg ctg ctg aac gcc 504

WO 01/85772 PCT/GB01/02003

Leu Asp Glu Gly Leu Leu Asn Ala 165 <210> 42 <211> 168 <212> PRT <213> Neisseria meningitidis <400> 42

Met Pro Leu Leu Asp Ser Phe Lys Val Asp His Thr Arg Met His Ala 1 5 10 15 Pro Ala Val Arg Val Ala Lys Thr Met Thr Thr Pro Lys Gly Asp Thr 20 - 25 30 Ile Thr Val Phe Asp Leu Arg Phe Cys Val Pro Asn Lys Glu Ile Leu 35 40 45 Pro Glu Lys Gly Ile His Thr Leu Glu His Leu Phe Ala Gly Phe Met 50 55 60 Arg Asp His Leu Asn Gly Asn' Gly Val Glu Ile Ile Asp Ile Ser Pro 65 70 75 80 Met Gly Cys Arg Thr Gly Phe Tyr Met Ser Leu Ile Gly Thr Pro Ser 85 90 95 Glu Gin Gin Val Ala Asp Ala Trp Leu Ala Ser Met Gin Asp Val Leu 100 105 110 Asn Val Lys Asp Gin Ser Lys Ile Pro Glu Leu Asn Glu Tyr Gin Cys 115 120 125 Gly Thr Tyr Gin Met His Ser Leu Ala Glu Ala Gin Gin Ile Ala Gin 130 135 140 Asn Val Leu Ala Arg Lys Val Ala Val Asn Lys Asn Glu Glu Leu Thr 145 150 155 160 Leu Asp Glu Gly Leu Leu· . Asn Ala

165 <210> 43 <211> 813 <212> DNA ·· ·· 49 ·

4 9 9 9 9 9 99

9 9 9 9 9

9 9 9 9 · 9

9 4 -4 4 9

9994 4994 44 444

49 944 9

4 9 9

4 9 9

9 4 · • β 9 9

44 49

WO 01/85772 PCT/GBO1/02003 <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(813) <400> 43

atg act atg cac His gcc ctc ccc cgc tac gcc gtt ttt ggc aac ccc gtc Val 48 Met Thr 1 Met Ala 5 Leu Pro Arg Tyr Ala 10 Val Phe Gly Asn Pro 15 gcc cac agc aaa tcg ccg caa att cat caa caa ttt gcc ctt cag gaa 96 Ala His Ser Lys Ser Pro Gin Ile His Gin Gin Phe Ala Leu Gin Glu 20 25 30 ggc gtt gac att gaa tac gaa cgc att tgc gcc gac atc ggc ggt ttc 144 Gly Val Asp Ile Glu Tyr Glu Arg Ile Cys Ala Asp Ile Gly Gly Phe 35 40 45 gcg cag gcg gtt tcg aca ttt ttt gaa aca ggc ggt tgc ggg gca aac 192 Ala Gin Ala Val Ser Thr Phe Phe Glu Thr Gly Gly Cys Gly Ala Asn 50 55 60 gtt ann rrta 9 ~ — ttc aarf —»3 *> A Λ ~ ~~ 9 -”-3 3—·— 3*^3 3*3 3“^v 3 «3 **** Val Thr Val Pro Phe Lys Gin GlU Ala Phe His Leu Ala Asp Glu His 65 70 75 80 tet gaa cgc gca ttg get gcc ggc gcg gtc aac acg ctg att ttt ctg 288 Ser GlU Arg Ala Leu Ala Ala Gly Ala Val Asn Thr Leu Ile Phe Leu 85 90 95 aaa aac gga aaa ctg cgc ggc gac aat acc gac ggt atc ggt ttg gcc 336 Lys Asn Gly Lys Leu Arg Gly Asp Asn Thr Asp Gly Ile Gly Leu Ala 100 105 110 aac gac atc acg cag gtc aaa aac att gcc atc gaa ggc aaa acc atc 384 Asn Asp Ile Thr Gin Val Lys Asn Ile Ala Ile Glu Gly Lys Thr Ile 115 120 125 ttg ctt ttg ggc gcg ggc ggc gcg gtg cgc ggc gtg att cct gtt ttg 432 Leu Leu Leu Gly Ala Gly _sGly Ala Val Arg Gly val Ile Pro Val Leu 130 135 140 aaa gaa cac cgt cct gcc cgt atc gtc att gcc aac cgt acc cgc gcc 480 Lys GlU His Arg Pro Ala Arg Ile Val Ile Ala Asn Arg Thr Arg Ala 145 150 155 160 aaa gcc gag gaa ttg gcg cag ctt ttc ggc att gaa gcc gtc ccg atg 528

·· ·*·· ·· ·· ·· · • · « ♦. · · ·· • · · · · • » · · · · • · · β e ···· ···· «· ··· • · · • · · • · · β e * β «· ··

WO 01/85772 PCT/GB01/02003 Lys Ala Glu Glu Leu Ala Gin Leu Phe Gly Ile Glu Ala Val Pro Met 165 170 175 gcg gat gtg aac ggc ggt ttt gat atc atc atc aac ggc acg tcg ggc 576 Ala Asp Val Asn Gly Gly Phe Asp Ile Ile Ile Asn Gly Thr Ser Gly 180 185 190 ggt cta aac ggt cag att ccc gat att ccg ccc gat att ttt caa aac 624 Gly Leu Asn Gly Gin Ile Pro Asp Ile Pro Pro Asp Ile Phe Gin Asn 195 200 205 tgc gcg ctt gcc tac gat atg gtg tac ggc tgc gcg gca aaa ccg ttt 672 cys Ala Leu Ala Tyr Asp Met Val Tyr Gly Cys Ala Ala Lys Pro Phe 210 215 220 tta gat ttt gca ega caa tcg ggt gcg aaa aaa act gcc gac gga ctg 720 Leu ZX«r> - Phe Ala Arg Gin Ser Gly Ala Lys Lys rnw v. m ~ ru.a Asp Gly Leu 225 230 235 240 ggt atg cta gtc ggt caa gcg gcg gct tcc tac gcc ctc tgg cgc gga 768 Gly Met Leu Val Gly Gin Ala Ala Ala Ser Tyr Ala Leu Trp Arg Gly 245 250 255 ttt acg ccc gat atc cgc ccc gtt atc gaa tac atg aaa gcc cta 813 Phe Thr Pro Asp Ile Arg Pro Val Ile Glu Tyr Met Lys Ala Leu 260 265 270

<210> 44 <211> 271 <212> PRT <213> Neisseria meningitidis <400> 44

Met Thr Met His Ala Leu Pro Arg Tyr Ala Val Phe Gly Asn Pro Val 1 5 10 15

Ala His Ser Lys Ser Pro Gin Ile His Gin Gin Phe Ala Leu Gin Glu 20 25 30

Gly Val Asp Ile Glu Tyr'Glu Arg Ile Cys Ala Asp Ile Gly Gly Phe 35 40 45

Ala Gin Ala Val Ser Thr Phe Phe Glu Thr Gly Gly Cys Gly Ala Asn 50 55 60

Val Thr Val Pro Phe Lys Gin Glu Ala Phe His Leu Ala Asp Glu His 65 70 75 80 • · • · · · · 9 · · • 4 β 4 · ···· • · · · · · · · ' 4 4 · · 4 · · · 4 4 · · · · · 4 9

W0 01/85772 PCT/GB01/02003 Ser Glu Arg Ala Leu 85 Ala Ala Gly Ala Val 90 Asn Thr Leu Ile Phe 95 Leu Lys Asn Gly Lys 100 Leu Arg Gly Asp Asn 105 Thr Asp Gly Ile Gly 110 Leu Ala Asn Asp Xle 115 Thr Gin Val Lys Asn 120 Ile Ala Ile Glu Gly 125 Lys Thr Ile Leu Leu 130 Leu Gly Ala Gly Gly 135 Ala Val Arg Gly Val 140 Ile Pro Val Leu Lys 145 Glu His Arg Pro Ala 150 Arg Ile Val Ile Ala 155 Asn Arg Thr Arg Ala 160 Lys Ala Glu Glu Leu 165 Ala Gin Leu Phe Gly 170 Ile Glu Ala Val P22C 175 Met Ala Asp Val Asn 180 Gly Gly Phe Asp Ile 185 Ile Ile Asn Gly Thr 190 Ser Gly Gly Leu Asn 195 Gly Gin Ile Pro Asp 200 Ile Pro Pro Asp Ile 205 Phe Gin Asn Cys Ala 210 Leu Ala Tyr Asp Met 215 Val Tyr Gly Cys Ala 220 Ala Lys Pro Phe Leu 225 Asp Phe Ala Arg Gin 230 Ser Gly Ala Lys Lys 235 Thr Ala Asp Gly Leu 240 Gly Met Leu Val Gly 245 Gin Ala Ala Ala Ser 250 Tyr Ala Leu Trp Arg 255 Gly Phe Thr Pro Asp 260 Ile Arg Pro Val Ile 265 Glu Tyr Met Lys Ala 270 Leu

<210> 45 <211> 546 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(546) • <5

WO 01/85772 PCT/GB01/02003 <400> 45 ttg ctt tet cat ttg gat atg aaa ttc gtc agc gac ctt ttg tet gtc 48 Leu Leu Ser His Leu Asp Met Lys Phe Val Ser Asp Leu Leu Ser Val 1 5 10 15 atc ttg ttt ttt gct act tat acc gtt acc aaa aat atg att gcc gct 96 Ile Leu Phe Phe Ala Thr Tyr Thr Val Thr Lys Asn Met Ile Ala Ala 20 25 30 acg gcg gtt gcc ttg gtt gcc ggt gtg gtt cag gcg gct ttt ctg tat 144 Thr Ala Val Ala Leu Val Ala Gly Val Val Gin Ala Ala Phe Leu Tyr 35 40 45 tgg aaa tat aaa aag ctg gat acg atg cag tgg gtc gga ctg gtg ctg 192 Trp Lys Tyr Lys Lys Leu Asp Thr Met Gin Trp Val Gly Leu Val Leu 50 55 60 att gtc gta ttc ggc ggc gca acc att gtt ttg ggc gac agc cgc ttc 240 Ile Val Val Phe Gly Gly Ala Thr Ile Val Leu Gly Asp Ser Arg Phe 65 70 75 80 att atg tgg aag ccg agc gtt ttg ttt tgg ctg ggc gcg ctg ttc ctg 288 Ile Met Trp Lys Pro Ser Val Leu Phe Trp Leu Gly Ala Leu Phe Leu 85 90 95 tgg ggc agc cac ctc gcc ggt aaa aac ggc ttg aag gcg agt atc ggc 336 Trp Gly Ser His Leu Ala Gly Lys Asn Gly Leu Lys Ala Ser Ile Gly 100 105 110 agg gag att cag ctt ccg gat gcc gta tgg gcg aaa ttg acg tat atg 384 Arg Glu Ile Gin Leu Pro Asp Ala Val Trp Ala Lys Leu Thr Tyr Met 115 120 125 tgg gtc ggt ttc ctg att ttt atg ggt atc gcc aac tgg ttt gtg ttt 432 Trp Val Gly Phe Leu Ile Phe Met Gly Ile Ala Asn Trp Phe Val Phe 130 135 140 acc cgg ttc gag tcg caa tgg gtc aac tat aaa atg ttc ggc tcg act 480 Thr Arg Phe Glu Ser Gin Trp Val Asn Tyr Lys Met Phe Gly Ser Thr 145 150 - 155 160 gca ctg atg ctt gtt ttc ttt att att cag ggt att tat ctg agt acc 528 Ala Leu Met Leu Val Phe Phe Ile Ile Gin Gly Ile Tyr Leu Ser Thr 165 170 175 tgt ctg aaa aag gag gat 546 Cys Leu Lys Lys Glu Asp

180 • · · ·

0 · «Β 0 · © · · · ········ ·· »·Ό ·· ··

WO 01/85772 PCT/GB01/02003 <210> 46 <211> 182 <212> PRT <213> Neisseria meningitidis <400> 46

Leu 1 Leu Ser His Leu Asp Met Lys Phe Val Ser Asp Leu Leu Ser Val 5 10 15 Ile Leu Phe Phe Ala Thr Tyr Thr Val Thr Lys Asn Met Ile Ala Ala 20 25 30 Thr Ala Val Ala Leu Val Ala Gly Val Val Gin Ala Ala Phe Leu Tyr 35 40 45 Trp Lys Tyr Lys Lys Leu Asp Thr Met Gin Trp Val Gly Leu Val Leu 50 55 60 Ile Val Val Phe Gly Gly Ala Thr Ile Val Leu Gly Asp Ser Arg Phe 65 70 75 80 Ile Met Trp Lys Prn Ser Val Leu Phe Trn Leu Rl v --- Ale Leu Phe Leu 85 90 95- Trp Gly Ser His Leu Ala Gly Lys Asn Gly Leu Lys Ala Ser Ile Gly 1.00 105 110 Arg Glu Ile Gin Leu Pro Asp Ala Val Trp Ala Lys Leu Thr Tyr Met 115 120 125 Trp Val Gly Phe Leu Ile Phe Met Gly Ile Ala Asn Trp Phe Val Phe 130 135 140 Thr Arg Phe Glu Ser Gin Trp Val Asn Tyr Lys Met Phe Gly Ser Thr 145 150 155 160 Ala Leu Met Leu Val Phe Phe Ile Ile Gin Gly Ile Tyr Leu Ser Thr 165 170 175 Cys Leu Lys Lys Glu Asp

<210> 47 <211> 585

180 «

WO 01/85772 PCT/GBO1/02003 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(585) <400> 47

ttg aat att aaa ctg Leu 5 aaa acc ttg tta ttg ccc ttc gcc acg ctg gca 48 Leu 1 Asn Ile Lys Lys Thr Leu Leu Leu Pro 10 Phe Ala Thr Leu 15 Ala ttg tgc acc aat get ttt gcc gcc ccg ccc age gac gcg tcg ttg gcg 96 Leu Cys Thr Asn Ala Phe Ala Ala Pro Pro Ser Asp Ala Ser Leu Ala 20 25 30 cgt tgg ctg gat acg cag aat ttt gac cgg gat ata gaa aat atg 1 Λ Λ Att Arg Trp Leu Asp Thr Gin Asn Phe Asp Arg Asp Ile Glu Lys Asn Met 35 40 45 att gag ggc ttt aat gcc gga ttt aaa ccg tat gcg gac aaa gcc ctt 192 Ile Glu Gly Phe Asn Ala Gly Phe Lys Pro Tyr Ala Asp Lys Ala Leu 50 55 60 gcc gaa atg ccg gaa gcg aaa aaa gat cag gcg gca gaa gcc ttt aac 240 Ala Glu Met Pro GlU Ala Lys Lys Asp Gin Ala Ala Glu Ala Phe Asn 65 70 75 80 cgt tat cgt gag aat gtt ttg aaa gat ttg att acg ccc gaa gtg aaa 288 Arg Tyr Arg Glu Asn Val Leu Lys Asp Leu Ile Thr Pro Glu Val Lys 85 90 95 cag get gtc cgc aat act tta ttg aag aat gcc cgt gag ata tac acg 336 Gin Ala Val Arg Asn Thr Leu Leu Lys Asn Ala Arg Glu Ile Tyr Thr 100 105 110 caa gaa gaa att gac ggc atg att gcc ttt tac ggt tcg cct gtc ggt 384 Gin Glu Glu Ile Asp Gly Met Ile Ala Phe Tyr Gly Ser Pro Val Gly 115 120 125 cag tcc gtc gtt gcc aaa aat ccg cgc tta atc aag aaa tcg atg agt 432 Gin Ser Val Val Ala Lys Asn Pro Arg Leu Ile Lys Lys Ser Met Ser 130 135 140 gaa ata gcg gta tet tgg act gca ttg tea ggg aaa atc gcg caa cat 480 Glu Ile Ala Val Ser Trp Thr Ala Leu Ser Gly Lys Ile Ala Gin His 145 150 155 160

ί

WO 01/85772

PCT/GB01/02003

cat ctg ccc gag His Leu Pro Glu ttt acg gaa gag ttg Glu Leu Phe 165 Thr Glu aaa aat ccc gat gcg ggc tgt aaa caa Lys Asn Pro Asp Ala Gly Cys Lys Gin 180 185 cat cag aaa His Gin Lys 195

cgg cgc atc atc tgc ggc ggt Arg Arg Ile Ile cys Gly Gly 170 175 gcc gga cag gtt ggg aaa agg Ala Gly Gin Val Gly Lys Arg 190

585 <210> 48 <211> 195 <212> PRT

<213> Neisseria <400> 48 Tngn j_ng c s Leu 1 Asn Ile Lys Leu 5 Lys Thr Leu Leu Leu 10 Pro Phe Ala Thr Leu 15 Ala Leu Cys Thr Asn 20 Ala Phe Ala Ala Pro 25 Pro Ser Asp Ala Ser 30 Leu Ala Arg Trp Leu 35 Asp Thr Gin Asn Phe 40 Asp Arg Asp Ile Glu 45 Lys Asn Met Ile Glu 50 Gly Phe Asn Ala Gly 55 Phe Lys Pro Tyr Ala 60 Asp Lys Ala Leu Ala 65 Glu Met Pro Glu Ala 70 Lys Lys Asp Gin Ala 75 Ala Glu Ala Phe Asn 80 Arg Tyr Arg Glu Asn 85 Val Leu Lys Asp Leu 90 Ile Thr Pro Glu Val 95 Lys Gin Ala Val Arg 100 Asn Thr Leu Leu Lys 105 Asn Ala Arg Glu Ile 110 Tyr Thr Gin Glu Glu 115 Ile Asp Gly Met Ile 120 Ala Phe Tyr Gly Ser 125 Pro Val Gly Gin Ser 130 Val Val Ala Lys Asn 135 Pro Arg Leu Ile Lys 140 Lys Ser Met Ser Glu 145 Ile Ala Val Ser Trp 150 Thr Ala Leu Ser Gly 155 Lys Ile Ala Gin His 160

WO 01/85772 PCT/GB01/02003

His Leu Pro Glu Phe Thr Glu Glu Leu Arg Arg Ile Ile Cys Gly Gly

165 170 ' 175

Lys Asn Pro Asp Ala Gly Cys Lys Gin Ala Gly Gin Val Gly Lys Arg

180 185 190

His Gin Lys 195 <210> 49 <211> 462 <212> DNA <213> Neisseria meningitidis <220> .

<221> CDS <222> (1)..(462) <400> 49

ttg ctt tgc ccg gaa aaa atg tcg ggg atg gcg gga cag tat ccg tac 48 Leu 1 Leu Cys Pro Glu Lys Met 5 Ser Gly Met Ala 10 Gly Gin Tyr Pro Tyr 15 ggc gtc cgg tcg ggt ttg cgg agg aac ggc ttg aaa ctt tgg gat att 96 Gly Val Arg Ser Gly Leu Arg Arg Asn Gly Leu Lys Leu Trp Asp Ile 20 25 30 cat ttt aga atg acc cgt ttt atc gtc gca aga tgc ggt tta ttg ttt 144 His Phe Arg Met Thr Arg Phe Ile Val Ala Arg Cys Gly Leu Leu Phe 35 40 45 gca acc ctt aaa gga aaa acc atg aag aaa atg ttc gtg ctg ttc tgt 192 Ala Thr Leu Lys Gly Lys Thr Met Lys Lys Met Phe Val Leu Phe Cys 50 55 60 atg ctg ttc tcc tgc gcc ttc tcc ctt gcg gcg gta aac atc aat gcg 240 Met Leu Phe Ser Cys Ala Phe Ser Leu Ala Ala Val Asn Ile Asn Ala 65 70- 75 80 get tcg cag cag gag ctg gag gcg ctg ccg ggc ata ggc cct gcg aag 288 Ala Ser Gin Gin Glu Leu Glu Ala Leu Pro Gly Ile Gly Pro Ala Lys 85 90 95 gcg aag gcc att gcg gaa tac cgt gcg caa aac ggt gcg ttc aag tet 336 Ala Lys Ala Ile Ala Glu Tyr Arg Ala Gin Asn Gly Ala Phe Lys Ser

WO 01/85772

·· fr· • · · * • · • · • fr • fr • • · • ·· frfr fr · • · • · ·«·· ···· • ii fr· fr ··· • · · • fr fr

PCT/GB01/02003

100 105 110 gta gac gat ttg acc aag gta aag ggc atc ggc cct gcg gtg ctg gcg 384 Val Asp Asp Leu Thr Lys Val Lys Gly Xle Gly Pro Ala Val Leu Ala 115 120 125 aag ctg aag gat cag get tet gtc ggt gcg ccc gca cca aaa ggc cca 432 Lys Leu Lys Asp Gin Ala Ser Val Gly Ala Pro Ala Pro Lys Gly Pro 130 135 140 get aaa cca gtg ctg ccc gcg gat aaa aaa 462 Ala Lys Pro Val Leu Pro Ala Asp Lys Lys 145 150

<210> 50 <211> 154 <212> PRT

<213> Neisseria meningitidis <400> 50 Leu 1 Leu Cys Pro Glu 5 Lys Met Ser Gly Met 10 Ala Gly Gin Tyr Pro 15 Tyr Gly Val Arg Ser 20 Gly Leu Arg Arg Asn 25 Gly Leu Lys Leu Trp Asp 30 Ile His Phe Arg 35 Met Thr Arg Phe Ile 40 Val Ala Arg Cys Gly 45 Leu Leu Phe Ala Thr 50 Leu Lys Gly Lys Thr 55 Met Lys Lys Met Phe 60 Val Leu Phe Cys Met 65 Leu Phe Ser Cys Ala 70 Phe Ser Leu Ala Ala 75 Val Asn Ile Asn Ala 80 Ala Ser Gin Gin Glu 85 Leu Glu Ala Leu Pro 90 Gly Ile Gly Pro Ala 95 Lys Ala Lys Ala Ile 100 Ala Glu Tyr Arg Ala 105 Gin Asn Gly Ala Phe 110 Lys Ser Val Asp Asp 115 Leu Thr Lys Val Lys 120 Gly Ile Gly Pro Ala 125 Val Leu Ala Lys Leu 130 Lys Asp Gin Ala Ser 135 Val Gly Ala Pro Ala 140 Pro Lys Gly Pro

WO 01/85772 φ ' φ φ φ · e φ · ΰ ·

9 Φ φ φ φ · φ φ · · · I » φ « <

• φ « * φ « Φ

PCT/GBO1/02003

Ala Lys Pro Val Leu Pro Ala Asp Lys Lys

145 150 <210> 51 <211> 969 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(969) <400> 51

atg tcc gcc aag ttc caa caa gaa acc ctc aaa tcc cgt ttc gcg caa 48 Mst 1 Ser 7% T — Λία Lys Phe Gin Gin Glu Thr Leu Lys ser Arg Phe Ala 15 Gin 5 10 gcc aaa gtc ctg gtt gtc ggc gac gtg atg ctc gac cgc tát tgg ttc 96 Ala Lys Val Leu Val Val Gly Asp Val Met Leu Asp Arg Tyr Trp Phe 20 25 30 ggc gat gtg tcc cgt att tcg ccc gaa gcc ccc gtg ccg gtg gcg aaa 144 Gly Asp Val Ser Arg Ile Ser Pro Glu Ala Pro Val Pro Val Ala Lys 35 40 45 atc gga ega atc gac caa cgc gcg ggc gga gcg gca aat gtc gcg cgc 192 Ile Gly Arg Ile Asp Gin Arg Ala Gly Gly Ala Ala Asn val Ala Arg 50 55 60 aac atc gct tcg ctg ggc ggc aaa gta ggg ctg ttg tcg gta acc ggt 240 Asn Ile Ala Ser Leu Gly Gly Lys Val Gly Leu Leu Ser Val Thr Gly 65 70 75 80 aac gac gaa gcc gcc gac gcg ctc gac gcg ctg atg gtg cag gac ggc 288 Asn Asp Glu Ala Ala Asp Ala Leu Asp Ala Leu Met Val Gin Asp Gly 85 90 95 gtc gcc tcc tat ctg atg ,cgc gac aaa caa atc gcc acc acc gtc aaa 336 Val Ala Ser Tyr Leu Meť Arg Asp Lys Gin Ile Ala Thr Thr Val Lys 100 105 110 ctg cgc gtc gtc gcc cgc aac cag cag ctt atc cgc ctt gat ttt gaa 384 Leu Arg Val Val Ala Arg Asn Gin Gin Leu Ile Arg Leu Asp Phe Glu 115 120 125 gaa cat ccc aac cgc gaa gtg ttg gaa caa atc aag cgg aaa tac cgc 432

WO 01/85772

PCT/GB01/02003

Glu His 130 Pro gaa atc ttg Glu 145 Xle Leu ggc ggc ctg Gly Gly Leu ggc aaa acc Gly Lys Thr gcc ggc gca Ala Gly Ala 195 gtc ggc age Val Gly 210 Ser rt cgc Leu 225 Arg Arg gaa ggc atg Glu Gly Met cgc gcc caa Arg Ala Gin gcc' gga atg Ala Gly Met 275 atg tac ctt Met Tyr 290 Leu acg gcg gtt Thr 305 Ala Val tea aca atg

Asn Arg Glu Val 135 Leu Glu Gin Ile Lys 140 Arg Lys Tyr Arg ccc gaa tac gac gca atc att ttt tea gac tac ggc aaa 480 Pro Glu Tyr Asp 150 Ala Ile Ile Phe 155 Ser Asp Tyr Gly Lys 160 tcg cac atc tcc gat atg atc gat tgg gcg aaa. cac gaa 528 Ser His 165 Ile Ser Asp Met Ile no Asp Trp Ala Lys His 175 Glu gta tta atc gac ccc aaa ggc gac gat tac gaa aaa tac 576 Val 180 Leu Ile Asp Pro Lys 185 Gly Asp Asp Tyr Glu 190 Lys Tyr acc Ctg att acg ccc aac cgc gcc gaa ttg aaa gaa gtg 624 Thr Leu Ile Thr Pro 200 Asn Arg Ala Glu Leu 205 Lys Glu Val tgg aaa aac gaa aat gat ttg acc gaa aaa gcc caa aac 672 Trp Lys Asn Glu 215 Asn Asp Leu Thr Glu 220 Lys Ala Gin Asn vaL· — X» vv- gac ttg ČtCC get att tta ctg acc ega age gaa 720 His Leu Asp 230 Leu Thr Ala Ile Leu 235 Leu Thr Arg Ser Glu 240 acc ttg ttc age gaa ggc gaa ccc att tac cag ccc acc 768 Thr Leu 245 Phe Ser Glu Gly Glu 250 Pro Ile Tyr Gin Pro 255 Thr gaa gtt tac gac gta tqc ggt gcg ggc gac acc gtc att 816 Glu 260 Val Tyr Asp Val Ser 265 Gly Ala Gly Asp Thr 270 Val Ile ggc ttg ggt ttg gcg gca ggc tgc acc atg ccc gaa gcc 864 Gly Leu Gly Leu Ala 280 Ala Gly Cys Thr Met 285 Pro Glu Ala gcc aat act gcg gcc ggg gtt gtc gtg gcg aaa ctc ggt 912 Ala Asn Thr, )Ala 295 Ala Gly Val Val Val 300 Ala Lys Leu Gly tgc tcg ttt gcc gaa ttg acc aag gca ttg tea ggg caa 960 Cys Ser Phe 310 Ala Glu Leu Thr Lys 315 Ala Leu Ser Gly Gin 320

969

·· <· ♦ · · « • · · ·· ···· • · ·' • « « · • · • · « * ' · V «· · · · · · 4» • * « · » « · · ♦

WO 01/85772

PCT/GBO1/O2OO3

Ser Thr Met <210> 52 <211> 323 <212> PRT <213> Neisseria meningitidis <400> 52

Met 1 Ser Ala Lys Phe 5 Gin Gin Glu Thr Leu 10 Lys Ser Arg Phe Ala 15 Gin Ala Lys Val Leu 20 Val Val Gly Asp Val 25 Met Leu Asp Arg Tyr 30 Trp Phe Gly Asp Val 35 Ser Arg Ile Ser Pro ’ Λ Λ •jy Glu Ala Pro Val Pro 45 Val Ala Lys Ile Gly Arg 50 Ile Asp Gin Arg 55 Ala Gly Gly Ala Ala 60 Asn Val Ala Arg Asn 65 Ile Ala Ser Leu Gly 70 Gly Lys Val Gly Leu 75 Leu Ser Val Thr Gly 80 Asn Asp Glu Ala Ala 85 Asp Ala Leu Asp Ala 90 Leu Met Val Gin Asp 95 Gly Val Ala Ser Tyr 100 Leu Met Arg Asp Lys 105 Gin Ile Ala Thr Thr 110 Val Lys Leu Arg Val 115 Val Ala Arg Asn Gin 120 Gin Leu Ile Arg Leu 125 Asp Phe Glu Glu His 130 Pro Asn Arg Glu Val 135 Leu Glu Gin Ile Lys 140 Arg Lys Tyr Arg Glu 145 Ile Leu Pro Glu Tyr 150 Asp Ala Ile Ile Phe 155 Ser Asp Tyr Gly Lys 160 Gly Gly Leu Ser His 165 Ile ,Ser Asp Met Ile 170 Asp Trp Ala Lys His 175 Glu Gly Lys Thr Val 180 Leu Ile Asp Pro Lys 185 Gly Asp Asp Tyr Glu 190 Lys Tyr Ala Gly Ala Thr Leu Ile Thr Pro Asn Arg Ala Glu Leu Lys Glu Val

195 200 205 • · · · » · * · · • · ·

WO 01/85772 PCT/GBO1/02003 Val Gly Ser Trp Lys Asn Glu Asn Asp Leu Thr 210 215 Glu Lys Ala Gin Asn 220 Leu Arg Arg His Leu Asp Leu Thr Ala Ile Leu 225 230 235 Leu Thr Arg Ser Glu 240 Glu Gly Met Thr Leu Phe Ser Glu Gly Glu Pro 245 250 Ile Tyr Gin Pro Thr 255 Arg Ala Gin Glu Val Tyr Asp Val Ser Gly Ala 260 265 Gly Asp Thr Val Ile 270 Ala Gly Met Gly Leu Gly Leu Ala Ala Gly Cys 275 280 Thr Met Pro Glu Ala 285 Met Tyr Leu Ala Asn Thr Ala Ala Gly Val Val 290 295 Val Ala Lys Leu Gly 300 Thr Ala Val Cys Ser Phe Ala Glu Leu Thr Lys 305 310 315 Ala Leu Ser Gly Gin 320

Ser Thr Met <210> 53 <211> 864 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(864) <400> 53 atg aaa gca aaa atc ctg act tcc gtt gca ctg ctt gcc tgt tcc ggc 48 Met 1 Lys Ala Lys Ile 5 Leu Thr Ser Val Ala 10 Leu Leu Ala Cys Ser 15 Gly agc ctg ttt gcc caa acg 'ctg gca acc gtc aac ggt cag aaa atc gac 96 Ser Leu Phe Ala 20 Gin Thr Leu Ala Thr 25 Val Asn Gly Gin Lys 30 Ile Asp agt tcc gtc att gat gcg cag gtt gcc gca ttc cgt gcg gaa aac agc 144 Ser Ser Val 35 Ile Asp Ala Gin Val 40 Ala Ala Phe Arg Ala 45 Glu Asn Ser

WO 01/85772 ·· «·

PCT/GB01/02003

cgt gcc gaa gac acg ccg caa ctg cgc caa tcc ctg ctg gaa aac gaa Glu 192 Arg Ala 50 Glu Asp Thr Pro Gin 55 Leu Arg Gin Ser Leu 60 Leu Glu Asn gtg gtc aac acc gtg gtc gca cag gaa gtg aaa cgc ctg aaa ctc gac 240 Val Val 65 Asn Thr Val Val 70 Ala Gin Glu Val Lys Arg 75 Leu Lys Leu Asp 80 cgg tcg gca gag ttt aaa aat gcg ctt gcc aaa ttg cgt gcc gaa gcg 288 Arg Ser Ala Glu Phe 85 Lys Asn Ala Leu Ala 90 Lys Leu Arg Ala Glu 95 Ala aaa aag tcg ggc gac gac aag aaa ccg tcc ttc aaa acc gtt tgg cag 336 Lys Lys Ser Gly 100 Asp Asp Lys Lys Pro Ser 105 Phe Lys Thr Val Trp 110 Gin gcg gta αάά tat ggc ttg aac ggc gag gca tac gcg ctg cat atc gcc 384 Ala Val Lys 115 Tyr Gly Leu Asn Gly Glu Ala 120 Tyr Ala Leu 125 His Ile Ala aaa acc caa ccg gtt tcc gag cag gaa gta aaa gcc gca tat gac aat 432 Lys Thr 130 Gin Pro Val Ser Glu 135 Gin Glu Val Lys Ala 140 Ala Tyr Asp Asn atc age ggt ttt tac aaa ggt acg cag gaa gtc cag ttg ggc gaa atc 480 Ile Ser 145 Gly Phe Tyr Lys 150 Gly Thr Gin Glu Val Gin 155 Leu Gly Glu Ile 160 ctg acc gac aag gaa gaa aat gca aaa aaa gcg gtt gcc gac ttg aag 528 Leu Thr Asp Lys Glu 165 Glu Asn Ala Lys Lys 170 Ala Val Ala Asp Leu 175 Lys gcg aaa aaa ggt ttc gat gcc gtc ttg aaa caa tat tcc ctc aac gac 576 Ala Lys Lys Gly 180 Phe Asp Ala Val Leu Lys 185 Gin Tyr Ser Leu Asn 190 Asp cgt acc aaa cag acc ggt gcg ccg gtc gga tat gtg ccg ctg aaa gat 624 Arg Thr Lys 195 Gin Thr Gly Ala Pro Val Gly 200 Tyr Val Pro 205 Leu Lys Asp ttg gaa cag ggt gtt ccg' ccg ctt tat cag gca att aag gac ttg aaa 672 Leu Glu 210 Gin Gly Val Pro Pro 215 Leu Tyr Gin Ala Ile 220 Lys Asp Leu Lys aaa ggc gaa ttt acg gca acg ccg ctg aaa aac ggc gat ttc tac ggc 720 Lys Gly 225 Glu Phe Thr Ala 230 Thr Pro Leu Lys Asn Gly Asp 235 Phe Tyr Gly 240

100 ® 4

WO 01/85772 PCT/GBO1/02003 gtt tat tat gtc aac gac age cgc gag gta aaa gtg cct tet ttt gat 768 Val Tyr Tyr Val Asn Asp Ser Arg Glu Val Lys Val Pro Ser Phe Asp 245 250 255 gaa atg aaa gga cag att gcg ggc aac ctt cag gcg gaa cgg att gac 816 Glu Met Lys Gly Gin Ile Ala Gly Asn Leu Gin Ala Glu Arg Ile Asp 260 265 270 cgt gcc gtc ggt gca ctg ttg ggc aag gca aac atc aaa cct gca aaa 864 Arg Ala Val Gly Ala Leu Leu Gly Lys Ala Asn Ile Lys Pro Ala Lys 275 280 285 <210> 54 <211> 288 <212> PRT <213> Neisseria meningitidis ' <400> 54 Met Lys Ala Lys Ile Leu Thr Ser Val Ala Leu Leu Ala Cys Ser Gly 1 5 10 15 Ser Leu Phe Ala Gin Thr Leu Ala Thr Val Asn Gly Gin Lys Ile Asp 20 25 30 Ser Ser Val Ile Asp Ala Gin Val Ala Ala Phe Arg Ala Glu Asn Ser 35 40 45 Arg Ala Glu Asp Thr Pro Gin Leu Arg Gin Ser Leu Leu Glu Asn Glu 50 55 60 Val Val Asn Thr Val Val Ala Gin Glu Val Lys Arg Leu Lys Leu Asp 65 70 75 80 Arg Ser Ala Glu Phe Lys Asn Ala Leu Ala Lys Leu Arg Ala Glu Ala 85 90 95 Lys Lys Ser Gly Asp Asp Lys Lys Pro Ser Phe Lys Thr Val Trp Gin 100 105 110 Ala Val Lys Tyr Gly Leu'Asn Gly Glu Ala Tyr Ala Leu His Ile Ala 115 120 125 Lys Thr Gin Pro Val Ser Glu Gin Glu Val Lys Ala Ala Tyr Asp Asn 130 135 140 Ile Ser Gly phe Tyr Lys Gly Thr Gin Glu Val Gin Leu Gly Glu Ile 145 150 155 160

101

fcfc fcfc • fc .· • fc fcfcfcfc fc fcfc · fc fc fcfc •^ř · fc • · • fcfc fc fcfc fc fc'··.· fcfcfcfc • · fcfcfc ·· fcfc

WO 01/85772

PCT/GBO1/02003

Leu Thr Asp Lys Glu Glu Asn Ala Lys Lys Ala Val Ala Asp Leu Lys 165 170 175

Ala Lys Lys Gly Phe Asp Ala Val Leu Lys Gin Tyr Ser Leu Asn Asp 180 185 190

Arg Thr Lys Gin Thr Gly Ala Pro Val Gly Tyr Val Pro Leu Lys Asp 195 200 205

Leu Glu Gin Gly Val Pro Pro Leu Tyr Gin Ala Xle Lys Asp Leu Lys 210 215 220

Lys Gly Glu Phe Thr Ala Thr Pro Leu Lys Asn Gly Asp Phe Tyr Gly

225 230 235 240

Val Tyr Tyr Val Asn Asp Ser Arg Glu val Lys Val Pro Ser Phe Asp

245 250 255

Glu Met Lys Gly Gin Ile Ala Gly Asn Leu Gin Ala Glu Arg Ile Asp 260 265 270

Arg Ala Val Gly Ala Leu Leu Gly Lys Ala Asn Ile tys pro ai = rys 275 280 285 <210> 55 <211> 1257 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1257) <400> 55

atg aaa cag acc gtc ctc aaa aat aac ctg caa aac ctg ctt gaa agc 48 Met Lys Gin Thr Val Leu Lys Asn Asn Leu Gin Asn Leu Leu Glu Ser 1 5 « 10 15 gca gaa aat atc ctg ctg ctt caa ggc cct gtc ggc gat ttt ttt ctg 96 Ala Glu Asn Ile Leu Leu Leu Gin Gly Pro Val Gly Asp Phe Phe Leu 20 25 30 cgc ctt gcc gac tgg ctg act gca aac ggc aaa acc gta cat aaa ttc 144 Arg Leu Ala Asp Trp Leu Thr Ala Asn Gly Lys Thr Val His Lys Phe 35 40 45

102

WO 01/85772 PCT/GB01/02003 • to · to · to to ······ • to · · · · to · · · to * '· . · · * · · · to · · · i ' «i · * to • to toto · · ·· · · ··· <· < to

aac ttt aat gca ggc Gly gac Asp gac tat ttt tat ccg ccc act caa gcg cat 192 Asn Phe 50 Asn Ala Asp 55 Tyr Phe Tyr Pro Pro 60 Thr Gin Ala His acc gtt gtt ttt aac gac aac tac gat gcc ttt cct gag ttt ttg caa 240 Thr Val Val Phe Asn Asp Asn Tyr Asp Ala Phe Pro Glu Phe Leu Gin 65 70 75 80 gaa tac atc act caa cat cac atc cag gcc gtt gtc tgc ttt ggc gac 288 Glu Tyr Ile Thr Gin His His Ile Gin Ala Val Val Cys Phe Gly Asp 85 90 95 aca cgc cct tat cac gtc att gca aaa cgc att gca aac gaa aac caa 336 Thr Arg Pro Tyr His Val Ile Ala Lys Arg Ile Ala Asn Glu Asn Gin 100 105 110 gcc agt ttc tgg gcg ttt gaa gaa ggc tat ttc cgc ccc tac tac atc 384 Ala Ser Phe Trp Ala Phe Glu Glu Gly Tyr Phe Arg Pro Tyr Tyr Ile 115 120 125 acc tta gaa aaa gac ggc gtc aac gca ttt tcc ccg ttg ccg cgc cgt 432 Thr Leu Glu Lys Asp Gly Val Asn Ala Phe Ser Pro Leu Pro Arg Arg 130 135 140 gcc gac ttt ttt ctt gaa caa ttc cct aag ctt gcc cag caa gaa tat 480 Ala Asp Phe Phe Leu Glu Gin Phe Pro Lys Leu Ala Gin Gin Glu Tyr 145 150 155 160 aaa gcg cca acg ccg gta cac ggc ggt ttt acg ccc atg gca aaa aac 528 Lys Ala Pro Thr Pro Val His Gly Gly Phe Thr Pro Met Ala Lys Asn 165 170 175 gct atc cgt tac tat atc gag ttg ttc cgc aat cta cgc aaa tac ccc 576 Ala Ile Arg Tyr Tyr Ile Glu Leu Phe Arg Asn Leu Arg Lys Tyr Pro 180 185 190 gac tac atc cac cac cgc gca ccc aat gcc ggc cat tac ctc aaa ccg 624 Asp Tyr Ile His His Arg Ala Pro Asn Ala Gly His Tyr Leu Lys Pro 195 - 200 205 tgg tcg ctc tcc atc ctc aag cgt ttg aac tac tat att gaa gac atc 672 Trp Ser Leu Ser Ile Leu Lys Arg Leu Asn Tyr Tyr Ile Glu Asp Ile 210 215 220 caa atc gcc aaa cgt gtg gaa gca ggc aaa tac ggc aag ttt ttt att 720 Gin Ile Ala Lys Arg Val Glu Ala Gly Lys Tyr Gly Lys Phe Phe Ile 225 230 235 240

103

PCT/GBO1/02003 » ·« · · ··

WO 01/85772 gtt ccc ttg cag gta ttc aac gac age Val Pro Leu Gin Val Phe Asn Asp Ser 245 ttt ccc age gtc cgc age ttc ctg ctc Phe Pro Ser Val Arg Ser Phe Leu Leu 260 265 gaa cac gcg cct gcc gat acc aac atc Glu His Ala Pro Ala Asp Thř Asn Ile 275 280 gac cgc ggc ttt atc gac tac tgg cgc Asp Arg Gly Phe Ile Asp Tyr Trp Arg 290 295 gaa cac ccc gaa ctc aaa ggc cgt gtg Glu His Pro Glu Leu Lys Gly Arg Val 305 310 ctg ccc gtt ttc ctg cgc cac ggt ctc Leu Pro Val Phe Leu Arg His Gly Leu 325 acc age ggc ctg tcc gga ctg att cac Thr Ser Gly Leu Ser Gly Leu Ile His 340 345 ggc cgt gcc tat tat gat att ccc ggc Gly Arg Ala Tyr Tyr Asp Ile Pro Gly 355 360 gca gaa ttt tgg aat cat ccg aca ccg Ala Glu Phe Trp Asn His Pro Thr Pro 370 375 gcc tac ega atg tac cac ctc aac gtg Ala Tyr Arg Met Tyr His Leu Asn Val 385 390 - tac agt cag gtc ttt ttt cct aac aaa Tyr Ser Gin Val Phe Phe Pro Asn Lys 405 cca aca acc Pro Thr Thr

caa gtc cgt atc cat tgc gac 768 Gin 250 Val Arg Ile His Cys 255 Asp cat gtt ttg agt tca ttt gcc 816 His Val Leu Ser Ser 270 Phe Ala atc atc aag cat cat cct atg 864 Ile Ile Lys His 285 His Pro Met gac att aaa cgc ttt atc aaa 912 Asp Ile Lys 300 Arg Phe Ile Lys att tat gtc cat gat gtc ccc 960 Ile Tyr 315 Val His Asp Val Pro 320 ggc atg gtt acc atc aac age 1008 Gly 330 Met Val Thr Ile Asn 335 aat atg cca gtt aag gtt ctc 1056 Asn Met Pro Val Lys 350 Val Leu att act gac caa aat acc ttg 1104 Ile Thr Asp Gin 365 Asn Thr Leu cct gac aaa gag ctg ttc cat 1152 Pro Asp Lys 380 Glu Leu Phe His acc caa att aac ggc aac ttc 1200 Thr Gin 395 Ile Asn Gly Asn Phe 400 aac aca tcc gac tet tcc aca 1248 Asn 410 Thr Ser Asp Ser Ser 415 Thr

1257

104 ·· 4444

WO 01/85772 PCT/GB01/02Q03

4 4 ·· ·4 • · 4 4 4 4

4 «44

4 4 · · 4

4 4 4 ·

44444444 44 <210> 56 <211> 419 <212> PRT <213> Neisseria meningitidis <400> 56

Met 1 Lys Gin Thr Val 5 Leu Lys Asn Asn Leu Gin Asn Leu Leu Glu Ser 10 15 Ala Glu Asn Xle Leu 20 Leu Leu Gin Gly Pro Val 25 Gly Asp Phe Phe Leu 30 Arg Leu Ala Asp Trp 35 Leu Thr Ala Asn Gly Lys 40 Thr Val His Lys Phe 45 Asn Phe 50 Asn Ais. Gly Asp Asp Tyr Phe Tyr Pro 55 Pro Thr Gin Ala His 60 Thr 65 Val Val Phe Asn Asp 70 Asn Tyr Asp Ala Phe 75 Pro Glu Phe Leu Gin 80 Glu Tyr Ile Thr Gin 85 His His Ile Gin Ala Val 90 Val Cys Phe Glv asn 95 Thr Arg Pro Tyr His 100 val Ile Ala Lys Arg Ile 105 Ala Asn Glu Asn Gin 110 Ala Ser Phe Trp Ala 115 Phe Glu Glu Gly Tyr Phe 120 Arg Pro Tyr Tyr Ile 125 Thr Leu 130 Glu Lys Asp Gly Val Asn Ala Phe Ser 135 Pro Leu Pro Arg Arg 140 Ala 145 Asp Phe Phe Leu Glu 150 Gin Phe Pro Lys Leu 155 Ala Gin Gin Glu Tyr 160 Lys Ala Pro Thr Pro 165 Val His Gly Gly Phe Thr 170 Pro Met Ala Lys Asn 175 Ala Xle Arg Tyr Tyr 180 Ile Glu Leu Phe Arg Asn 185 Leu Arg Lys Tyr Pro 190 Asp Tyr Ile His His 195 Arg Ala Pro Asn Ala Gly 200 His Tyr Leu Lys Pro 205 Trp Ser 210 Leu Ser Ile Leu Lys Arg Leu Asn Tyr 215 Tyr Ile Glu Asp Ile 220

105 ·· 9999 • 9

WO 01/85772 PCT/GB01/02003 ·· ·· 99

9 9 9 9 9 9 9

9 9 · ·

9 9 9··

9 9 9 9 • 999 9999 99 9

Gin 225 Ile Ala Lys Arg Val 230 Glu Ala Gly Lys Tyr 235 Gly Lys Phe Phe Ile 240 Val Pro Leu Gin Val 245 Phe Asn Asp Ser Gin 250 Val Arg Ile His Cys 255 Asp Phe Pro Ser val 260 Arg Ser Phe Leu Leu 265 His Val Leu Ser Ser 270 Phe Ala Glu His Ala 275 Pro Ala Asp Thr Asn 280 Ile Ile Ile Lys His 285 His Pro Met Asp Arg 290 Gly Phe Xle Asp Tyr 295 Trp Arg Asp Ile Lys 300 Arg Phe Ile Lys Glu 305 His Pro Glu Leu Lys 310 Gly Arg Val Ile Tyr 315 Val His Asp Val Pro 320 Leu Pro Val Phe Leu 325 Arg His Gly Leu Gly 330 Met Val Thr Ile Asn 335 Ser Thr Ser Gly T»eu 340 m«» ——j Leu TI « tl( « 345 n naii X- ťiCL Pro Val Lys 350 Val Leu Gly Arg Ala 355 Tyr Tyr Asp Ile Pro 360 Gly Ile Thr Asp Gin 365 Asn Thr Leu Ala Glu 370 Phe Trp Asn His Pro 375 Thr Pro Pro Asp Lys 380 Glu Leu Phe His Ala 385 Tyr Arg Met Tyr His 390 Leu Asn Val Thr Gin 395 Ile Asn Gly Asn Phe 400 Tyr Ser Gin Val Phe Phe Pro Asn Lys Asn Thr Ser Asp Ser Ser Thr

405 410 415

Pro Thr Thr <210> 57 <211> 1407 <212> DNA <213> Neisseria meningitidis <220>

106 ·« ···· ·· ·· ·· • · · · · · · · φ · ·

9 · · · · · · «

4 949 4 944 9 4

4 4 4 4 4 9 4 4 9

4994 4444 ·· 444 44 ··

WO 01/85772 PCT/GB01/02003 <221> CDS <222> (1)..(1407) <400> 57

atg Met 1 acc tcc aca ttc ccc cgc cgc ctc gcc cgc aaa atc cgc caa acc 48 Thr Ser Thr Phe Pro Arg 5 Arg Leu Ala 10 Arg Lys Ile Arg Gin 15 Thr cgc cgc ctg tcg cgc aaa age atc gcc ttt ctg ttc ctt ttg’ gca ggt 96 Arg Arg Leu Ser Arg Lys Ser Ile Ala Phe Leu Phe Leu Leu Ala Gly 20 25 30 tcg gca ctc gtc gcc ctg acc gcg ctg ttt ttt gcc cat ctt gcc gat 144 Ser Ala Leu Val Ala Leu Thr Ala Leu Phe Phe Ala His Leu Ala Asp 35 40 45 ttt gcg ctg gaa ctg aac gcc aáa ctg gtt caa caa tac ccg tgg ttc 192 Phe Ala Leu Glu Leu Asn Ala Lys Leu Val Gin Gin Tyr Pro Trp Phe 50 55 60 gcg tgg gtc gcg ctt cct ttg ggt tta ccg ctt att gcg tgg ctc aca 240 Ala Trp Val Ala Leu Pro Leu Gly Leu Pro Leu Ile Ala Trp Leu Thr 65 70 75 _ ...... 80 cgc aaa ttc gcc ccc ttc acc gcc ggc age ggc atc ccg cag gtc atc 288 Arg Lys Phe Ala Pro Phe Thr Ala Giy Ser Gly Ile Pro Gin Val Ile 85 90 95 gcc tea ctg tcg ctg ccc tac ggc gca cag aaa acg cgg ctg atc cgc 336 Ala Ser Leu Ser Leu Pro Tyr Gly Ala Gin Lys Thr Arg Leu Ile Arg 100 105 110 ctc ggg cag acg ctg ctg aag att ccg cta acc ttt ttg ggt atg ctg 384 Leu Gly Gin Thr Leu Leu Lys Ile Pro Leu Thr Phe Leu Gly Met Leu 115 120 125 ttc ggc gcg tcc atc gga cgc gaa ggt ccg tcc gtg cag gtc ggc gcg 432 Phe Gly Ala Ser Ile Gly Arg Glu Gly Pro Ser Val Gin Val Gly Ala 130 135 140 gca gtg atg ggc gcg tgg 'ggc gcg tgg tgc aag aaa cac ggc ttg gca 480 Ala Val Met Gly Ala Trp Gly Ala Trp Cys Lys Lys His Gly Leu Ala 145 150 155 160 ttc aaa ggg atg cag gaa aac gat ttg atg gcg gcg ggc gcg gcg ggc 528 Phe Lys Gly Met Gin Glu Asn Asp Leu Met Ala Ala Gly Ala Ala Gly 165 170 175

107 ·· ··«* * · ·

WO 01/85772

ggt ttg gca gcc gcg ttc aac gcg ccg Gly Leu Ala Ala 180 Ala Phe Asn Ala Pro 185 att gag gaa ctc ggg cgc ggc atc atg Ile Glu Glu 195 Leu Gly Arg Gly Ile 200 Met ctt ttg ggc gtg ctc gcc tcc ggt ttc Leu Leu 210 Gly Val Leu Ala Ser 215 Gly Phe aac aac ccg tat ttt tcc ggc ttc aac Asn 225 Asn Pro Tyr Phe Ser 230 Gly Phe Asn ttt ctg tgg gtc gca ctg tcc ggc ctg Phe Leu Trp Val Ala 245 Leu Ser Gly Leu ctg ttc gga cgt ttg ctc tat cgc ggt Leu Phe Gly Arg X· Λ Leu Leu Tyr Arg Gly 265 aag ata cgc ggc ttc atc cgc aac cgt Lys Ile Arg 275 Gly Phe Ile Arg Asn 280 Arg atg ggg ctg ctg ctc gcc ctg ctc ggc Met Gly 290 Leu Leu Leu Ala Leu 295 Leu Gly tac ggc acc ggc tac cac gaa gcc gcc Tyr 305 Gly Thr Gly Tyr His 310 Glu Ala Ala gaa gcc ccc ttc gga ctc gcc gcc gcc Glu Ala Pro Phe Gly 325 Leu Ala Ala Ala agc tat tgg gca ggc gtt ccg ggc ggc Ser Tyr Trp Ala 340 Gly Val Pro Gly Gly 345 ata ggc gcg gtt ttg ggc gag cat atc Ile Gly Ala 355 Val Leu Gly Glu His 360 Ile

PCT/GB01/02003 ctg gcg ggc gtg att ttc gcc 576 Leu Ala Gly Val Ile 190 Phe Ala ttg cgc tgg gag agg caa att 624 Leu Arg Trp GlU 205 Arg Gin Ile ata cag gtc gcc att cag ggc 672 Ile Gin Val 220 Ala Ile Gin Gly ggc ggc gta ttg gaa cat atc 720 Gly Gly 235 Val Leu Glu His Ile 240 gtt tgc ggc gcg gcg ggc ggg 768 Val 250 Cys Gly Ala Ala Gly 255 Gly gcg gcg gcg ttt gca ccg cgc 816 Ala Ala Ala Phe Ala 270 Pro Arg ccg ctg ctg ctg gcg gca ctg 864 Pro Leu Leu Leu 285 Ala Ala Leu acg ttc tac caa ggc aaa acc 912 Thr Phe Tyr 300 Gin Gly Lys Thr caa gcc ctg cac ggc atc tac 960 Gin Ala 315 Leu His Gly Ile Tyr 320 aaa tgg ctc gcc acc gta ttc 1008 Lys 330 Trp Leu Ala Thr Val 335 Phe att ttc act ccc tcg ctg acc 1056 Ile Phe Thr Pro Ser 350 Leu Thr gcc gcc atc gcc gac ata tcg 1104 Ala Ala Ile Ala Asp Ile Ser

365

108

WO 01/85772 PCT/GBO1/02003 ·· ·· fl· • fl · · flfl fl fl · · • · · · · • · · fl • flflfl ···· ·· • fl ·*·· • · · • flfl flfl* · • · · · flfl flfl

cag ggt Gin Gly 370 gca aac atc Ala Asn Ile atc gtc ctc atc tgc atg gcg gca ttt ctg gcg 1152 Ile Val 375 Leu Ile Cys Met Ala Ala Phe Leu 380 Ala ggc gcg aca caa tcc ccg att act tcc gcc gtc gtc gtc atg gaa atg 1200 Gly Ala Thr Gin Ser Pro Ile Thr Ser Ala Val Val Val Met Glu Met 385 390 395 400 acg ggc gga caa agc ctg ctg ttt tgg atg ctg att gcč tgc att ttc 1248 Thr Gly Gly Gin Ser Leu Leu Phe Trp Met Leu Ile Ala Cys Ile Phe 405 410 415 gcc tcg cag gtt tcg cgc cag ttt tcg ccg cgt ccg ttc tac cac gca 1296 Ala. Ser Gin Val Ser Arg Gin Phe Ser Pro Arg Pro Phe Tyr His Ala 420 425 430 tcg gga atg Ζ'ΖΎΛ· ttc cgc Cay G€)C gtg ctt caa gaa acc gcc gcc caa 1344 Ser Gly Met Arg Phe Arg Gin Arg Val Leu Gin Glu Thr Ala Ala Gin 435 440 445 acc ggc aat gcg ccc gca aga ccg caa aca gca aac agc aaa acg gga 1392 Thr Gly Asn Ala Pro Ala Arg Pro Gin Thr Ala Asn Ser Lys Thr Gly

450 455 460 atg ccg tet gaa aat 1407

Met Pro Ser Glu Asn

465 <210> 58 <211> 469 <212> PRT <213> Neisseria meningitidis <400> 58

Met 1 Thr Ser Thr Phe 5 Pro Arg Arg Leu Ala 10 Arg Lys Ile Arg Gin 15 Thr Arg Arg Leu Ser 20 Arg Lys Ser Ile Ala 25 Phe Leu Phe Leu Leu 30 Ala Gly Ser Ala Leu 35 Val Ala Leu Thr Ala 40 Leu Phe Phe Ala His 45 Leu Ala Asp Phe Ala 50 Leu Glu Leu Asn Ala 55 Lys Leu Val Gin Gin 60 Tyr Pro Trp Phe Ala Trp Val Ala Leu Pro Leu Gly Leu Pro Leu Ile Ala Trp Leu Thr

109 ·· ·· «· · • · · · » c ·· • · · · · • · · * · t o · · · · ···· ···· ·· ··· ···· ·· • · · • · · • · · • · · · ·· ··

WO 01/85772 65 70 75 Arg Lys Phe Ala Pro Phe Thr Ala Gly Ser Gly 85 90 Ala Ser Leu Ser Leu Pro Tyr Gly Ala Gin Lys 100 105 Leu Gly Gin Thr Leu Leu Lys Ile Pro Leu Thr 115 120 Phe Gly Ala Ser Ile Gly Arg Glu Gly Pro Ser 130 135 Ala Val Met Gly Ala Trp Gly Ala Trp Cys Lys 145 150 155 Phe Lys Gly Met Gin Glu Asn Asp Leu Met Ala 165 170 Gly Leu Ala Ala Ala Phe Asn Ala Pro Leu Ala 180 185 Ile Glu Glu Leu Gly Arg Gly Ile Met Leu Arg 195 200 Leu Leu Gly Val Leu Ala Ser Gly Phe Ile Gin 210 215 Asn Asn Pro Tyr Phe Ser Gly Phe Asn Gly Gly 225 230 235 Phe Leu Trp Val Ala Leu Ser Gly Leu Val Cys 245 250 Leu Phe Gly Arg Leu Leu Tyr Arg Gly Ala Ala 260 265 Lys Ile Arg Gly Phe Ile Arg Asn Arg Pro Leu 275 280 Met Gly Leu Leu Leu Ala Leu Leu Gly Thr Phe 290 295 Tyr Gly Thr Gly Tyr His GlU Ala Ala Gin Ala 305 / 310 315 Glu Ala Pro Phe Gly Leu Ala Ala Ala Lys Trp

Ile

Thr

Phe

Val

140

Lys

Ala

Gly

Trp

Val

220

Val

Gly

Ala

Leu

Tyr

300

Leu

Leu

PCT/GB01/02003

Pro Gin Val Ile 95

Arg Leu Ile Arg 110

Leu Gly Met Leu 125

Gin Val Gly Ala

His Gly Leu Ala 160

Gly Ala Ala Gly 175

Val Ile Phe Ala 190

Glu Arg Gin Ile 205

Ala Ile Gin Gly

Leu Glu His Ile 240

Ala Ala Gly Gly 255

Phe Ala Pro Arg 270

Leu Ala Ala Leu 285

Gin Gly Lys Thr

His Gly Ile Tyr 320

Ala Thr Val Phe

110 . 4. Jkř,

• · · · • · · · • · fr · fr • · • • • • · • • • ·· · • • • • • • • •, · • • • • • • · ·'· · · · · · · fr¹· • · · • · • ·

WO 01/85772 PCT/GBO1/02003 325 330 335

Ser Tyr Trp Ala 340 Gly Val Pro Gly Gly 345 Ile Phe Thr Pro Ser 350 Leu Thr Ile Gly Ala 355 Val Leu Gly Glu His 360 Ile Ala Ala Ile Ala 365 Asp Ile Ser Gin Gly 370 Ala Asn Ile Ile Val 375 Leu Ile Cys Met Ala 380 Alá Phe Leu Ala Gly 385 Ala Thr Gin Ser Pro 390 Ile Thr Ser Ala Val 395 Val Val Met Glu Met 400 Thr Gly Gly Gin Ser 405 Leu Leu Phe Trp Met 410 Leu Ile Ala Cys Ile 415 Phe Ala Ser Gin Val 420 Ser Arg Gin Phe Ser 425 Pro Arg Pro Phe Tyr 430 His Ala Ser Gly Met 435 Arg Phe Arg Gin Arg 440 Val Leu Gin Glu Thr 445 Ala Ala Gin Thr Gly Asn Ala Pro Ala Arg Pro Gin Thr Ala Asn Ser Lys Thr Gly

455

460

450

Met Pro Ser Glu Asn 465 <210> 59 <211> 423 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(423) <400> 59

atg aca caa gaa acc get ttg ggc Met 1 Thr Gin Glu Thr 5 Ala Leu Gly act atg age aaa aag aaa cag aca Thr Met Ser Lys 20 Lys Lys Gin Thr

gca gca ctg aaa tcc gcc gtc caa Ala Ala Leu Lys Ser Ala Val Gin 10 15 gaa atg att gcc gac cac atc tac Glu Met Ile Ala Asp His Ile Tyr 25 30

111

WO 01/85772 PCT/GB01/02003

• · · • · · • · • · · • · · • • 9 β • • • · • • • • • • • • • • • • ♦ • · • · · • · ·»

ggc aaa tat gat Tyr Asp 35 gta ttc aaa ega ttt aaa ccg ttg gca ctc ggt atc 144 Gly Lys Val Phe Lys Arg Phe Lys Pro Leu Ala Leu Gly Ile 40 45 gat cag gat ttg att gcc get ttg ccc caa tac gat gcc gca ctg att 192 Asp Gin Asp Leu Ile Ala Ala Leu Pro Gin Tyr Asp Ala Ala Leu Ile 50 55 ⁶⁰ gca cgc gtc ctc gcc aac cac tgc cgc cgt ccg cgc tat ctg aaa gcc 240 Ala Arg Val Leu Ala Asn His Cys Arg Arg Pro Arg Tyr Leu Lys Ala 65 70 75 80 ttg gcg cgc ggt ggc aaa cgt ttc gat ttg aac aac cgt ttc aaa ggc 288 Leu Ala Arg Gly Gly Lys Arg Phe Asp Leu Asn Asn Arg Phe Lys Gly 85 ⁹⁰ 95 gaa gtt acc ccc gaa gaa cag gca atc gcg caa aac cat cct ttc gtg 336 Glu Val Thr Pro Glu Glu Gin Ala Ile Ala Gin Asn His Pro Phe Val 100 105 110 cag cag get ttg caa cag cag tcc gcc caa get gtc gcc gaa acg ccg 384 Gin Gin Ala Leu Gin Gin Gin Ser Ala Gin Ala Val Ala Glu Thr Pro 115 120 125 tet gtt gaa gcc gaa gca gcc gaa tet tcc aca aca gaa 423 Ser Val Glu Ala Glu Ala Ala Glu Ser Ser Thr Thr Glu 130 135 140

<210> 60 <211> 141 <212> PRT <213> Neisseria meningitidis <400> 60

Met Thr Gin Glu Thr Ala Leu Gly Ala Ala Leu Lys Ser Ala Val Gin 15 10 15

Thr Met Ser Lys Lys Lys Gin Thr Glu Met Ile Ala Asp His Ile Tyr 20 25 30

Gly Lys Tyr Asp Val Phe Lys Arg Phe Lys Pro Leu Ala Leu Gly Ile 35 40 45

Asp Gin Asp Leu Ile Ala Ala Leu Pro Gin Tyr Asp Ala Ala Leu Ile 50 55 60

112 ··· 0 0 0 0 0 0 ·

0 000· 000 • · · · · 0 0 0 0 ·

0 0 ·* · .· *··

WO 01/85772

Ala Arg Val Leu Ala Asn His Cys Arg Arg Pro Arg 65 70 75

Leu Ala Arg Gly Gly Lys Arg Phe Asp Leu Asn Asn 85 90

Glu Val Thr Pro Glu Glu Gin Ala Ile Ala Gin Asn 100 105

Gin Gin Ala Leu Gin Gin Gin Ser Ala Gin Ala Val 115 120

Ser Val Glu Ala Glu Ala Ala Glu Ser Ser Thr Thr 130 135 140

PCT/GBO1/02003

Tyr Leu Lys Ala 80

Arg Phe Lys Gly 95

His Pro Phe Val 110

Ala Glu Thr Pro 125

Glu <210> 61 <211> 1377 <212> DNA <213> Neisseria meningitidis <22 0>

<221> CDS <222> (1)..(1377) <400> 61

atg Met 1 ttc get ttc aaa tcc tta ctc gat atg ccg cgc ggt gag gca ctt 48 Phe Ala Phe Lys Ser 5 Leu Leu Asp Met 10 Pro Arg Gly Glu Ala Leu 15 gcc gtc gtc gtc get ctg att gcc gcg atg ggc tat acc atc att tea 96 Ala Val Val Val Ala Leu Ile Ala Ala Met Gly Tyr Thr Ile Ile Ser 20 25 30 ttg gag tgg ctg ccg cat atg tcc att att gcc gcc atc gtc gtg ctg 144 Leu Glu Trp Leu Pro His Met Šer Ile Ile Ala Ala Ile val Val Leu 35 40 45 att ttg tac ggc ttg gcg cgc ggt ttg aaa tac aac gat atg cag cag 192 Ile Leu Tyr Gly Leu Ala Arg Gly Leu Lys Tyr Asn Asp Met Gin Gin 50 55 60 ggc atg ata ggc gcg ttg aat cag ggt atg ggc gcg att tac ctg ttt 240 Gly Met Ile Gly Ala Leu Asn Gin Gly Met Gly Ala Ile Tyr Leu Phe 65 70 75 80 ttc ttc atc ggg ctg atg gtc agc gcg ctg atg atg agc ggc gcg att 288

113 • · · ·

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Phe Phe Ile Gly Leu 85 Met Val Ser Ala Leu 90 Met Met Ser Gly Ala 95 Ile ccg acg ctg atg tat tac ggt ttc gga ctg att tcc ccg act tat ttt 336 Pro Thr Leu Met 100 Tyr Tyr Gly Phe Gly 105 Leu Ile Ser Pro Thr 110 Tyr Phe tat ttt tcc gcc ttc gcg ctg tgt tcc gtc atc ggc gtg tcc atc ggc 384 Tyr Phe Ser 115 Ala Phe Ala Leu Cys 120 Ser Val Ile Gly Val 125 Ser Ile Gly agc agc ctg acc acc tgc gcc act gtc ggc gtt gcc ttt atg ggt atg 432 Ser Ser 130 Leu Thr Thr Cys Ala 135 Thr Val Gly Val Ala 140 Phe Met Gly Met gcg gcg gcg ttt cag gcc gat atg gcg atg acg gcg ggc gcg att gtt 480 Ala 145 Ala Ala phe Gin Ala 150 Asp Met Ala Met Thr 155 Ala Gly Ala Ile Val 160 tcg ggc gca ttt ttt ggc gac aaa atg tcc ccg ctt tcc gat acg acg 528 Ser Gly Ala Phe Phe 165 Gly Asp Lys Met Ser 170 Pro Leu Ser Asp Thr 175 Thr ggc att tcc gcg tcc att gtc ggt atc gac ctg ttt gaa cac atc aaa 576 Gly Ile Ser Ala 180 Ser Ile Val Gly Ile 185 Asp Leu Phe Glu His 190 Ile Lys aat atg atg tac acc acc att ccc gcg tgg ctc atc agt gcg gca ctg 624 Asn Met Met 195 Tyr Thr Thr Ile Pro 200 Ala Trp Leu Ile Ser 205 Ala Ala Leu atg ctg tgg ctt ctt ccc agc gtc gcc gcg cag gat ttg aac agc gtc 672 Met Leu 210 Trp Leu Leu Pro Ser 215 Val Ala Ala Gin Asp 220 Leu Asn Ser Val gaa tcc ttc cgc agc cag ctt gaa gcc acg gga ttg gtg cac tgc tat 720 Glu 225 Ser Phe Arg Ser Gin 230 Leu Glu Ala Thr Gly 235 Leu Val His Cys Tyr 240 tcg ctg att ccg ttt gcg .ctg ttg gtc gtt ttg gca ttg atg cgc gtc 768 Ser Leu Ile Pro Phe 245 Ala Leu Leu Val Val 250 Leu Ala Leu Met Arg 255 Val aat gcc gtg gtc gct atg ctc ttt acc gtc att gcc gcc gtt gcc gta 816 Asn Ala Val Val 260 Ala Met Leu Phe Thr 265 Val Ile Ala Ala Val 270 Ala Val acg tat ctg cac agc acg ccc gat ctg cgt cag ctc ggc gcg tgg ttt 864

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WO 01/85772 PCT/GB01/02003 ···· β · · · « β · • · · · · ··· • · · · · · · · · · • « · β β β β · β

Thr Tyr Leu His Ser Thr Pro Asp Leu Arg Gin Leu Gly Ala Trp Phe 275 280 285 tac ggc ggc tac aaa ctc gaa ggc gaa gcg ttt aaa gac att gcc aaa 912 Tyr Gly Gly Tyr Lys Leu Glu Gly Glu Ala Phe Lys Asp Ile Ala Lys 290 295 300 ctc att tet cgc ggc ggt ttg gaa agt atg ttt ttc acg cag acc atc 960 Leu Ile Ser Arg Gly Gly Leu Glu Ser Met Phe Phe Thr Gin Thr Ile 305 310 315 320 gtg att ctt ggg atg age ctt ggc ggg ctg ctg ttt gca ctg ggc gcg 1008 Val Ile Leu Gly Met Ser Leu Gly Gly Leu Leu Phe Ala Leu Gly Ala 325 330 335 att cct tcc ctg ctg gat gcc gtc cgc age ttt ttg acg aat gcc ggg 1056 Ile Pro Ser Leu Leu Asp Ala Val Arg Ser Phe Leu Thr Asn Ala Gly 340 345 350 cgt gcc aca ttc age gtt gcc atg act tcg gtc ggg gtt aat ttc ctg 1104 Arg Ala Thr Phe Ser Val Ala Met Thr Ser Val Gly Val Asn Phe Leu 355 360 365 atc ggc gag caa tat ttg agt att ttg ttg tcg ggt gaa acg ttc aaa 1152 Ile Gly Glu Gin Tyr Leu Ser Ile Leu Leu Ser Gly Glu Thr Phe Lys 370 375 380 cct gtt tac gat aag ctc ggt ctg cat tcg cgc aat ctg tcg cgg acg 1200 Pro Val Tyr Asp Lys Leu Gly Leu His Ser Arg Asn Leu Ser Arg Thr 385 390 395 400 ctg gaa gat gcg ggg acg gtc atc aac ccg ctc gta ccg tgg age gta 1248 Leu Glu Asp Ala Gly Thr Val Ile Asn Pro Leu Val Pro Trp Ser Val 405 410 415 tgc ggc gtg ttc atc age cac gcg ctg ggc gtg ccg gtt tgg gaa tat 1296 Cys Gly Val Phe Ile Ser His Ala Leu Gly Val Pro Val, Trp Glu Tyr 420 425 430 ctg ccg tat gcc ttt ttc tgc tat ttg agt ttg gct ttg acc ctg tta 1344 Leu Pro Tyr Ala Phe Phe Cys Tyr Leu Ser Leu Ala Leu Thr Leu Leu 435 440 445 ttc ggt tgg acg ggg ctg act ttg age aaa aaa 1377 Phe Gly Trp Thr Gly Leu Thr Leu Ser Lys Lys 450 455

115 • · · ·

·· ·· • e β · • · • · ·· · · · · · · ·· · ······ 9 β · β · β β • · · · · · • · « · · · · ·· ··· ·· ·· WO 01/85772 PCT/GB01/02003 <210> 62 <211> 459 <212> PRT <213> Neisseria meningitidis <400> 62 Met Phe Ala Phe 1 Lys Ser Leu Leu Asp 5 Met 10 Pro Arg Gly Glu Ala 15 Leu

Ala Val Val Val 20 Ala Leu Ile Ala Ala 25 Met Gly Tyr Thr Ile 30 Ile Ser Leu Glu Trp 35 Leu Pro His Met Ser 40 Ile Ile Ala Ala Ile 45 Val Val Leu Ile Leu 50 Tyr Gly Leu Ala Arg 55 Gly Leu Lys Tyr Asn 60 Asp Met Gin Gin Gly 65 Met Ile Gly Ala Leu 70 Asn Gin Gly Met Gly 75 Ala Ile Tyr Leu Phe 80 Phe Phe Ile Gly Leu 85 Met Val Ser Ala Leu 90 Met Met Ser Gly Ala 95 Ile Pro Thr Leu Met 100 Tyr Tyr Gly Phe Gly 105 Leu Ile Ser Pro Thr 110 Tyr Phe Tyr Phe Ser 115 Ala Phe Ala Leu Cys 120 Ser Val Ile Gly Val 125 Ser Ile Gly Ser Ser 130 Leu Thr Thr Cys Ala 135 Thr Val Gly Val Ala 140 Phe Met Gly Met Ala 145 Ala Ala Phe Gin Ala 150 Asp Met Ala Met Thr 155 Ala Gly Ala Ile val 160 Ser Gly Ala Phe Phe 165 Gly Asp Lys Met Ser 170 Pro Leu Ser Asp Thr 175 Thr Gly Ile Ser Ala 180 Ser Ile .Val Gly Ile 185 Asp Leu Phe Glu His 190 Ile Lys Asn Met Met 195 Tyr Thr Thr Ile Pro 200 Ala Trp Leu Ile Ser 205 Ala Ala Leu Met Leu Trp Leu Leu Pro Ser Val Ala Ala Gin Asp Leu Asn Ser Val

210 215 220

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Glu Ser 225 Phe Arg Ser Gin Leu Glu Ala Thr Gly Leu Val His Cys Tyr 230 235 240 Ser Leu Ile Pro Phe Ala Leu Leu Val Val Leu Ala Leu Met Arg Val 245 250 255 Asn Ala Val Val Ala Met Leu Phe Thr Val Ile Ala Ala Val Ala Val 260 265 270 Thr Tyr Leu His Ser Thr Pro Asp Leu Arg Gin Leu Gly Ala Trp Phe 275 280 285 Tyr Gly Gly Tyr Lys Leu Glu Gly Glu Ala Phe Lys Asp Ile Ala Lys 290 295 300 Leu Ile Ser Arg Gly Gly Leu Glu Ser Met Phe Phe Thr Gin Thr Ile 305 310 315 320 Val Ile Leu Gly Met Ser Leu Gly Gly Leu Leu Phe Ala Leu Gly Ala 325 330 335 Ile Pro Ser Leu Leu Asp Ala Val Arg Ser Phe Leu Thr Asn Ala Gly 340 345 350 Arg Ala Thr Phe Ser Val Ala Met Thr Ser Val Gly Val Asn Phe Leu 355 360 365 Ile Gly Glu Gin Tyr Leu Ser Ile Leu Leu Ser Gly Glu Thr Phe Lys 370 375 380 Pro Val Tyr Asp Lys Leu Gly Leu His Ser Arg Asn Leu Ser Arg Thr 385 390 395 400 Leu Glu Asp Ala Gly Thr Val Ile Asn Pro Leu Val Pro Trp Ser Val 405 410 415 Cys Gly Val Phe Ile Ser His Ala Leu Gly Val Pro Val Trp Glu Tyr 420 425 430 Leu Pro Tyr Ala Phe Phe Cys Tyr Leu Ser Leu Ala Leu Thr Leu Leu 435 440 445 Phe Gly Trp Thr Gly Leu Thr Leu Ser Lys Lys

450 455 <210> 63

117 ·· ·· ·· · ·· ···· ········ · · ·

WO 01/85772 PCT/GB01/02003 <211> 1098 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1098, <400> 63

atg Met 1 gca ggc aac act ttc gga caa ctc ttc acc gtt acc acc ttc ggc 48 Ala Gly Asn Thr 5 Phe Gly Gin Leu Phe 10 Thr Val Thr Thr Phe 15 Gly gaa agc cac ggc gcg ggt ttg ggc tgt atc atc gac ggc tgc ccg ccc 96 Glu Ser His Gly Ala Gly Leu Gly Cys Ile Ile Asp Gly Cys Pro Pro 20 25 30 ggc ttg gaa tta agc gaa gcg gat atc caa ttt gac ctc gac ega cgc 144 Gly Leu Glu Leu Ser Glu Ala Asp Ile Gin Phe Asp Leu Asp Arg Arg 35 40 45 aaa ccc ggc acc agc cgc cac gtt acc caa cgc cgc gaa gcc gac caa 192 Lys Pro Gly Thr Ser Arg His Val Thr Gin Arg Arg Glu Ala Asp Gin 50 55 60 gtc gaa atc ctc tcc ggc gta ttc gaa ggc aaa acc acc ggc acg ccc 240 Val Glu Ile Leu Ser Gly Val Phe Glu Gly Lys Thr Thr Gly Thr Pro 65 70 75 80 atc gcc ctc tta atc cgc aat acc gac cag cgc agc aaa gac tac ggc 288 Ile Ala Leu Leu Ile Arg Asn Thr Asp Gin Arg Ser Lys Asp Tyr Gly 85 90 95 aac atc gcc acc agc ttc cgc ccc ggc cac gcc gac tat acc tat tgg 336 Asn Ile Ala Thr Ser Phe Arg Pro Gly His Ala Asp Tyr Thr Tyr Trp 100 105 110 cac aaa tac ggc acg cgc gac tac cgg ggc ggc ggc agg agt tcc gcc 384 His Lys Tyr Gly Thr Arg Asp Tyr Arg Gly Gly Gly Arg Ser Ser Ala 115 120 125 cgc gaa acc gcc gcc cgc gtt gcc gcc gga gcc gtt gcc aaa aaa tgg 432 Arg Glu Thr Ala Ala Arg Val Ala Ala Gly Ala val Ala Lys Lys Trp 130 135 140 ttg aaa gaa aaa ttc ggc acg gaa atc acc gcc tac gtt acc caa gtc 480 Leu Lys Glu Lys Phe Gly Thr Glu Ile Thr Ala Tyr Val Thr Gin Val 145 150 155 160

118

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ggc gaa aaa gaa atc cgg ttt gaa ggc tgc gaa cac att tcc caa aat 528 Gly Glu Lys Glu Ile Arg 165 Phe Glu Gly Cys 170 Glu His Ile Ser Gin Asn 175 cct ttt ttt gcc gcc aac cat agc caa att gcc gag ctg gaa aac tat 576 Pro Phe Phe Ala Ala Asn His Ser Gin Ile Ala Glu Leu Glu Asn Tyr 180 185 190 atg gac agc gtg cgc aaa tcc ttg gat tcc gtc ggc gcg aag ctg cat 624 Met Asp Ser Val Arg Lys Ser Leu Asp Ser Val Gly Ala Lys Leu His 195 200 205 att gaa gcc gcc aac gtc ccc gtc ggc ttg ggc gaa ccc gta ttc gac 672 Ile Glu Ala Ala Asn val Pro Val Gly Leu Gly Glu Pro Val Phe Asp 210 215 220 cgc ctc gat gcc gaa atc gcc tac gcc atg atg ggc atc aac gcc gtc 720 Arg Leu Asp Ala Glu Ile Ala Tyr Ala Met Met Gly Ile Asn Ala Val 225 230 235 240 aaa ggc gtg gaa atc ggc gca ggt ttt gac agc gta acg caa cgc ggc 768 Lys Gly Val Glu Ile Gly Ala Gly Phe Asp Ser Val Thr Gin Arg Gly 245 250 255 agc gaa cac ggc gac gaa ctc acc ccg caa ggc ttc ctg tcc aac cac 816 Ser Glu His Gly Asp Glu Leu Thr Pro Gin Gly Phe Leu Ser Asn His 260 265 270 tea ggc ggc atc ctc ggc ggc atc agc acc gga caa gac atc cac gtc 864 Ser Gly Gly Ile Leu Gly Gly Ile Ser Thr Gly Gin Asp Ile His Val 275 280 285 aat atc gcc atc aaa ccc acc agc tcc atc gcc acc ccg cgc cgc agt 912 Asn Ile Ala Ile Lys Pro Thr Ser Ser Ile Ala Thr Pro Arg Arg Ser 290 295 300 atc gac atc aac ggc aac ccc atc gaa ctc gcc acg cac ggc agg cac 960 Ile Asp Ile Asn Gly Asn Pro Ile Glu Leu Ala Thr His Gly Arg His 305 310 - 315 320 gac ccc tgc gtc ggc ttg cgc gcc gcg ccg att gca gaa gct atg ctg 1008 Asp Pro Cys Val Gly Leu Arg Ala Ala Pro Ile Ala Glu Ala Met Leu 325 330 335 gca ttg gtt tta atc gac cac gcc ctg cgc cat cgc gcg caa aat gcg 1056 Ala Leu Val Leu Ile Asp His Ala Leu Arg His Arg Ala Gin Asn Ala 340 345 350

119

WO 01/85772 PCT/GB01/02003

gat gtt cag gtt aat acg ccc gac att acc ctt tca aac aaa 1098 Asp Val Gin Val Asn Thr Pro Asp Ile Thr Leu Ser Asn Lys 355 360 365

<210> 64 <211> 366 <212> PRT <213> Neisseria meningitidis <400> 64

Met 1 Ala Gly Asn Thr 5 Phe Gly Gin Leu Phe 10 Thr Val Thr Thr Phe 15 Gly Glu Ser His Gly 20 Ala Gly Leu Gly Cys 25 Ile Ile Asp Gly Cys 30 Pro Pro Gly Leu Glu 35 Leu Ser Glu Ala Asp 40 Ile Gin Phe Asp Leu 45 Asp Arg Arg Lys Pro 50 Gly Thr Ser Arg His 55 Val Thr Gin Arg Arg 60 Glu Ala Asp Gin Val 65 Glu Ile Leu Ser Gly 70 Val Phe Glu Gly Lys 75 Thr Thr Gly Thr Pro 80 Xle Ala Leu Leu Ile 85 Arg Asn Thr Asp Gin 90 Arg Ser Lys Asp Tyr 95 Gly Asn Ile Ala Thr 100 Ser Phe Arg Pro Gly 105 His Ala Asp Tyr Thr 110 Tyr Trp His Lys Tyr 115 Gly Thr Arg Asp Tyr 120 Arg Gly Gly Gly Arg 125 Ser Ser Ala Arg Glu 130 Thr Ala Ala Arg Val 135 Ala Ala Gly Ala Val 140 Ala Lys Lys Trp Leu 145 Lys Glu Lys Phe Gly 150 .Thr Glu Ile Thr Ala 155 Tyr Val Thr Gin Val 160 Gly Glu Lys Glu Ile 165 Arg Phe Glu Gly Cys 170 Glu His Ile Ser Gin 175 Asn Pro Phe Phe Ala Ala Asn His Ser Gin Ile Ala Glu Leu Glu Asn Tyr

180 185 190

120 • · ··· ·

WO 01/85772 PCT/GB01/02003

Met Asp Ser Val 195 Arg Lys Ser Leu Asp Ser Val Gly Ala Lys Leu His 200 205 Ile Glu Ala Ala Asn Val Pro Val Gly Leu Gly Glu Pro Val Phe Asp 210 215 220 Arg Leu Asp Ala Glu Ile Ala Tyr Ala Met Met Gly ile Asn Ala Val 225 230 235 240 Lys Gly Val Glu Ile Gly Ala Gly Phe Asp Ser Val Thr Gin Arg Gly 245 250 255 Ser Glu His Gly Asp Glu Leu Thr Pro Gin Gly Phe Leu Ser Asn His 260 265 270 Ser Gly Gly Ile Leu Gly Gly Ile Ser Thr Gly Gin Asp Ile His Val 275 280 285 Asn Ile Ala Ile Lys Pro Thr Ser Ser Ile Ala Thr Pro Arg Arg Ser 290 295 300 Ile Asp Ile Asn Gly Asn Pro Ile Glu Leu Ala Thr His Gly Arg His 305 310 315 320 Asp Pro Cys Val Gly Leu Arg Ala Ala Pro Ile Ala Glu Ala Met Leu 325 330 335 Ala Leu Val Leu Ile Asp His Ala Leu Arg His Arg Ala Gin Asn Ala 340 345 350 Asp Val Gin Val Asn Thr Pro Asp Ile Thr Leu Ser Asn Lys 355 360 365

<210> 65 <211> 461 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(459) <400> 65 atg caa age ggt ttt aac gct att ttc agg aat gac acc atg cag gtt 48

Met Gin Ser Gly Phe Asn Ala Ile Phe Arg Asn Asp Thr Met Gin Val

1 5 10 15

121

WO 01/85772 PCT/GB01/02003

aca tea aaa tgg ata gac gga atg tgc ttt gtc gga aca acg gaa ggc 96 Thr Ser Lys Trp 20 Ile Asp Gly Met Cys Phe Val 25 Gly Thr Thr 30 Glu Gly ggg cac agc gtc gtt atg gag ggg tcg gcg gca gaa ggt gcg gcc aag 144 Gly His Ser Val Val Met Glu Gly Ser Ala Ala Glu Gly Ala Ala Lys 35 40 45 cgc ggg ccc agc cct ttg gaa atg ctg ctg ttg ggc gtg gcg ggc tgt 192 Arg Gly Pro Ser Pro Leu Glu Met Leu Leu Leu Gly Val Ala Gly Cys 50 55 60 tcg agc atc gat gtg gtg atg att gcc gaa aaa cag cgt cag aaa gtg 240 Ser Ser Ile Asp Val Val Met Ile Ala Glu Lys Gin Arg Gin Lys Val 65 70 75 80 act gac tgc cgt gcg acg gtt acg gcg aaa cgg gcg gac gat gcg ccg 288 Thr Asp Cys Arg Ala Thr Val Thr Ala Lys Arg Ala Asp Asp Ala Pro 85 90 95 cgc gtg ttt acc gaa atc cac atc cat ttc aaa gta ttc ggg cat gat 336 Arg Val Phe Thr Glu Ile His Ile His Phe Lys Val Phe Gly· His Asp 100 105 110 ttg aaa gaa tcg gcc att gag cgc gcc gtt cag atg tet gcc gaa aaa 384 Leu Lys Glu Ser Ala Ile Glu Arg Ala Val Gin Met Ser Ala Glu Lys 115 120 125 tac tgt tcg get tcg att atg ttg ggc aaa gcg gca aag att acc cac 432 Tyr Cys Ser Ala Ser Ile Met Leu Gly Lys Ala Ala Lys Ile Thr His 130 135 140 agt ttt gaa att gcc ggg gca gat áaa ta 461 Ser Phe Glu Ile Ala Gly Ala Asp Lys 145 150

<210> 66 <211> 153 <212> PRT <213> Neisseria meningitidis <400> 66

Met Gin Ser Gly Phe Asn Ala Xle Phe Arg Asn Asp Thr Met Gin Val 1 5 10 15

Thr Ser Lys Trp Ile Asp Gly Met Cys Phe Val Gly Thr Thr Glu Gly

122

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20 25 30

Gly His Ser 35 Val Val Met Glu Gly 40 Ser Ala Ala Glu Gly 45 Ala Ala Lys Arg Gly 50 Pro Ser Pro Leu Glu 55 Met Leu Leu Leu Gly 60 Val Ala Gly Cys Ser 65 Ser Ile Asp Val Val 70 Met Ile Ala Glu Lys 75 Gin Arg Gin Lys Val 80 Thr Asp Cys Arg Ala 85 Thr Val Thr Ala Lys 90 Arg Ala Asp Asp Ala 95 Pro Arg Val Phe Thr 100 Glu Ile His Ile His 105 Phe Lys Val Phe Gly 110 His Asp Leu Lys Glu 115 Ser Ala Ile Glu Arg 120 Ala Val Gin Met Ser 125 Ala Glu Lys Tyr Cys 130 Ser Ala Ser Ile Met 135 Leu Gly Lys Ala Ala 140 Lys Ile Thr His Ser 145 Phe Glu Ile Ala Gly 150 Ala Asp Lys

<210> 67 <211> 1659 <212> DNA <213> Nelsseria meningitidis <220>

<221> CDS <222> (1,..(1659, <400> 67 atg act gac aac agc cct cct cca aac gga cac gcc caa gca cgc gtc 48

Met Thr Asp Asn Ser Pro, Pro Pro. Asn Gly His Ala Gin Ala Arg Val

15 10 15 cgc aaa aac aac acc ttc ctc tet gcc gtc tgg ctg gtt ccg ctg atc 96

Arg Lys Asn Asn Thr Phe Leu Ser Ala Val Trp Leu Val Pro Leu Ile

20 25 30 gcg ctg att gcc ggc ggc tgg ctt tgg gtt aag gaa atc cgc aac agg 144

Ala Leu ile Ala Gly Gly Trp Leu Trp Val Lys Glu Ile Arg Asn Arg

123 φφ ·· ·· φ φ φ φφφ φ • φ φ * φ · φφ φφ φ φ φφφφ φφφ φ φφφφ φφφφ φ φ φ φφφ φφφφ φφφφ φφ·· φφ φφφ φφ φφ

W0 01/85772 PCT/GB01/02003

35 40 45 ggg cct gtg gtt acg ctc ttg atg gac age gcg gaa ggc att gag gtc 192 Gly Pro Val Val Thr Leu Leu Met Asp Ser Ala Glu Gly Ile Glu Val 50 55 60 aac aat acg gtc atc aaa gta ttg age atc gat gtc gga cgc gtt acc 240 Asn Asn Thr Val Ile Lys Val Leu Ser Ile Asp Val Gly Arg Val Thr 65 70 75 80 ega atc aaa ctg cgc gac gac caa aaa ggc gtg gaa gta acc gcc caa 288 Arg Ilé Lys Leu Arg Asp Asp Gin Lys Gly Val Glu Val Thr Ala Gin 85 90 95 ctc aat gcg gac gta tcc ggc ctc atc cgc age gat acc cag ttt tgg 336 Leu Asn Ala Asp Val Ser Gly Leu Ile Arg Ser Asp Thr Gin Phe Trp 100 105 110 gtg gtc aag ccg cgt atc gac caa age ggc gta acc ggt ttg ggt acg 384 Val Val Lys Pro Arg Ile Asp Gin Ser Gly Val Thr Gly Leu Gly Thr 115 120 125 ctg ctt tcg ggt tcg tac atc gcc ttt aca ccc. ggc aaa age gac gag 432 Leu Leu Ser Gly Ser Tyr Ile Ala Phe Thr Pro Gly Lys Ser Asp Glu 130 135 140 gca aaa gac gtg ttc caa gtg cag gac att ccg ccc gtt acc gcc atc 480 Ala Lys Asp Val Phe Gin val Gin Asp Ile Pro Pro Val Thr Ala Ile 145 150 155 160 ggg caa age ggg ctg cgc ttg aat ttg att ggt aaa aac gac cgc' atc 528 Gly Gin ser Gly Leu Arg Leu Asn Leu Ile Gly Lys Asn Asp Arg Ile 165 170 175 ctc aac gtc aac age cct gtt ttg tat gaa aac ttt atg gtc ggg caa 576 Leu Asn Val Asn Ser Pro Val Leu Tyr Glu Asn Phe Met Val Gly Gin 180 185 190 gtc gaa age gcg cat ttc gac ccg tcc gac caa age gtg cat tac acc 624 Val. Glu Ser Ala His Phe Asp Pro Ser Asp Gin Ser Val His Tyr Thr 195 200 205 atc ttc atc caa age ccc aac gac aaa ctg att cat tcc gcc age cgt 672 Ile Phe Ile Gin Ser Pro Asn Asp Lys Leu Ile His Ser Ala Ser Arg 210 215 220 ttc tgg ctg gaa age ggc atc aat atc gaa acc aca ggc age ggc atc 720 Phe Trp Leu Glu Ser Gly Ile Asn Ile Glu Thr Thr Gly Ser Gly Ile

124 ·* ····

WO 01/85772 PCT/GBO1/02003 •« · · ·« • · · · · · • · * · • · · ♦ · • · · · ··· · · · ·· ··

225 230 235 240 aaa ctc aat tcc gcc cct ctg cct gcc ctg ctg tcg ggc gcg att tca 768 Lys Leu Asn Ser Ala Pro Leu Pro Ala Leu Leu Ser Gly Ala Ile Ser 245 250 255 ttt gat tcg ccg aaa acc aaa aac agt aaa aac gtc aaa age gaa gac 816 Phe Asp Ser Pro Lys Thr Lys Asn Ser Lys Asn Val Lys Ser Glu Asp 260 265 270 age ttc acg ctt tac gac age cgc age gaa gtc gcc aac ctg cct gat 864 Ser Phe Thr Leu Tyr Asp Ser Arg Ser Glu Val Ala Asn Leu Pro Asp 275 280 285 gac cgt tcg ctg tac tac acc gcg ttt ttc aaa caa tcc gtg cgc gga 912 Asp Arg Ser Leu Tyr Tyr Thr Ala Phe Phe Lys Gin Ser Val Arg Gly 290 295 300 ctg acc gtc ggt tcg cct gtc gag tac aaa ggg ctg aat gtc ggc gtg 960 Leu Thr Val Gly Ser Pro Val Glu Tyr Lys Gly Leu Asn Val Gly Val 305 310 315 320 gtt tcc gat gtt cct tat ttc gac čgc aac gac age ctg cac ctg ttt 1008 Val Ser Asp Val Pro Tyr Phe Asp Arg Asn Asp Ser Leu His Leu Phe 325 330 335 gaa aac ggc tgg att ccc gta cgc atc cgt att gag cct tcc cgt ttg 1056 Glu Asn Gly Trp Ile Pro Val Arg Ile Arg Ile Glu Pro Ser Arg Leu 340 345 350 gaa atc aat gcc gac gaa caa age aaa gaa cat tgg áaa caa caa ttt 1104 Glu Ile Asn Ala Asp Glu Gin Ser Lys Glu His Trp Lys Gin Gin Phe 355 360 365 cag acg gcc tta aac aaa ggc ctg acc gcc acc » atc tcc age aac aac 1152 Gin Thr Ala Leu Asn Lys Gly Leu Thr Ala Thr Ile Ser Ser Asn Asn 370 375 380 ctg ctg acc ggc age aaa atg att gag ttg aac gat cag cct tcc gcc 1200 Leu Leu Thr Gly Ser Lys Met Ile Glu Leu Asn Asp Gin Pro Ser Ala 385 390 395 400 tcg ccc aag ctg ega ccg cat acc gtt tat gca ggc gat acc gtt atc 1248 Ser Pro Lys Leu Arg Pro His Thr Val Tyr Ala Gly Asp Thr Val Ile 405 410 415 gcg acc cag ggc ggc ggt ttg gac gat ttg cag gtc aaa ttg gcg gat 1296 Ala Thr Gin Gly Gly Gly Leu Asp Asp Leu Gin Val Lys Leu Ala Asp

125 ·· ·* * ·· ··«· • · · · · · ·« « · · « · · · · · « β « ··'·· · · · Λ φ • · * · · · · · · ···*···« ·« ··· ·· ··

WO 01/85772 PCT/GB01/02003

420 425 430 ttg ctg gac aag ttc gac aaa ctg cct tta gat aag acg gtt gcc gaa 1344 Leu Leu Asp Lys Phe Asp Lys Leu Pro Leu Asp Lys Thr Val Ala Glu 435 440 445 ttg aac ggt tcg ctt gcc gag ctc aaa tcc aca ctc aaa tet gcc aat 1392 Leu Asn Gly Ser Leu Ala Glu Leu Lys Ser Thr Leu Lys Ser Ala Asn 450 455 460 gcc gcc cta age tcc atc gac aaa ctg gtc ggc aaa ccg cag aca caa 1440 Ala Ala Leu Ser Sér Ile Asp Lys Leu val Gly Lys Pro Gin Thr Gin 465 470 475 480 aac att ccg aac gaa ctg aac caa acc ctg aaa gag ttg cgc aca acc 1488 Asn Ile Pro Asn Glu Leu Asn Gin Thr Leu Lys Glu Leu Arg Thr Thr 485 490 495 ctg caa ggc gta tcg cct caa tcg cct atc tac ggc gac gta caa aat 1536 Leu Gin Gly Val Ser Pro Gin Ser Pro Ile Tyr Gly Asp Val Gin Asn 500 505 510 acg ctg caa agt ttg gac aaa acc tta aaa gac gtt caa ccc gtc att 1584 Thr Leu Gin Ser Leu Asp Lys Thr Leu Lys Asp Val Gin Pro Val Ile 515 520 525 aac act ttg aaa gaa aaa ccc aac gcg ctg att ttc aac age age age 1632 Asn Thr Leu Lys Glu Lys Pro Asn Ala Leu Ile Phe Asn Ser Ser Ser 530 535 540 aaa gac cct atc ccg aaa gga age ega 1659 Lys Asp Pro Ile Pro Lys Gly Ser Arg

545 550 <210> 68 <211> 553 <212> PRT

<213> Neisseria meningitidis <400> 68 Met Thr Asp Asn Ser Pro Pro Pro Asn Gly His Ala Gin Ala Arg Val 15 10 15 Arg Lys Asn Asn Thr Phe Leu Ser Ala Val Trp Leu Val Pro Leu Ile 20 25 30 Ala Leu Ile Ala Gly Gly Trp Leu Trp Val Lys Glu Ile Arg Asn Arg

126 ·· ·· ·· · • · · · ·' · »« Λ » « • · · · · ··· « ··«· ···· »· ·»·· ···· ···· ·· ··· ·« «·

WO 01/85772

PCT/GBO1/02003

Gly Pro Val Val Thr Leu Leu Met Asp Ser Ala Glu Gly Ile Glu Val 50 55 60

Asn Asn Thr Val Ile Lys Val Leu Ser Ile Asp Val Gly Arg Val Thr

65 70 75 80

Arg Ile Lys Leu Arg Asp Asp Gin Lys Gly Val Glu Val Thr Ala Gin

85 90 95

Leu Asn Ala Asp Val Ser Gly Leu Ile Arg Ser Asp Thr Gin Phe Trp 100 105 110

Val Val Lys Pro Arg Ile Asp Gin Ser Gly Val Thr Gly Leu Gly Thr 115 120 125

Leu Leu Ser Gly Ser Tyr Ile Ala Phe Thr Pro Gly Lys Ser Asp Glu 130 135 140

Ala Lys Asp Val Phe Gin Val Gin Asp Ile Pro Pro Val Thr Ala Ile

145 150 155 160

Gly Gin Ser Gly Leu Arg Leu Asn Leu Ile Gly Lys Asn Asp Arg Ile

165 170 175

Leu Asn Val Asn Ser Pro Val Leu Tyr Glu Asn Phe Met Val Gly Gin 180 185 190

Val Glu Ser Ala His Phe Asp Pro Ser Asp Gin Ser Val His Tyr Thr 195 200 205

Ile Phe Ile Gin Ser Pro Asn Asp Lys Leu Ile His Ser Ala Ser Arg 210 215 220

Phe Trp Leu Glu Ser Gly Ile Asn Ile Glu Thr Thr Gly Ser Gly Ile

225 230 235 240

Lys Leu Asn Ser Ala Pro Leu Pro Ala Leu Leu Ser Gly Ala Ile Ser

245 , 250 255

Phe Asp Ser Pro Lys Thr Lys Asn Ser Lys Asn Val Lys Ser Glu Asp 260 265 270

Ser Phe Thr Leu Tyr Asp ser Arg Ser Glu Val Ala Asn Leu Pro Asp 275 280 285

Asp Arg Ser Leu Tyr Tyr Thr Ala Phe Phe Lys Gin Ser Val Arg Gly

127

Ά-.·. ·. Λ·.

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PCT/GB01/02003

290 295 300 Leu Thr Val Gly Ser Pro Val Glu Tyr Lys Gly Leu Asn Val Gly Val 305 310 315 320 Val Ser Asp Val Pro Tyr Phe Asp Arg Asn Asp Ser Leu His Leu Phe 325 330 335 Glu Asn Gly Trp Ile Pro Val Arg Ile Arg Ile Glu Pro Ser Arg Leu 340 345 350 Glu Ile Asn Ala Asp Glu Gin Ser Lys Glu His Trp Lys Gin .Gin Phe 355 360 365 Gin Thr Ala Leu Asn Lys Gly Leu Thr Ala Thr Ile Ser Ser Asn Asn 370 375 380 Leu Leu Thr Gly Ser Lys Met Ile Glu Leu Asn Asp Gin Pro Ser Ala 385 390 395 400 Ser Pro Lys Leu Arg Pro His Thr Val Tyr Ala Gly Asp Thr Val Ile 405 410 415 Ala Thr Gin Gly Gly Gly Leu Asp Asp Leu Gin Val Lys Leu Ala Asp 420 425 430 Leu Leu Asp Lys Phe Asp Lys Leu Pro Leu Asp Lys Thr Val Ala Glu 435 440 445 Leu Asn Gly ser Leu Ala Glu Leu Lys Ser Thr Leu Lys Ser Ala Asn 450 455 460 Ala Ala Leu Ser Ser Ile Asp Lys Leu Val Gly Lys Pro Gin Thr Gin 465 470 475 480 Asn Ile Pro Asn Glu Leu Asn Gin Thr Leu Lys Glu Leu Arg Thr Thr 485 490 495 Leu Gin Gly Val Ser Pro Gin Ser Pro Ile Tyr Gly Asp Val Gin Asn 500 505 510 Thr Leu Gin Ser Leu Asp Lys Thr Leu Lys Asp Val Gin Pro Val Ile 515 520 525 Asn Thr Leu Lys Glu Lys Pro Asn Ala Leu Ile Phe Asn Ser Ser Ser 530 535 540 Lys Asp Pro Ile Pro Lys Gly Ser Arg

128 • ·

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545 550 <210> 69 <211> 330 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(330) <400> 69

ttg ccg ctg ctt tgt Cys 5 tgt Cys ctt tgg ttt tgg cag Gin cct gcg gcg gtg aaa 48 Leu 1 Pro Leu Leu Leu Trp Phe Trp 10 Pro Ala Ala Val 15 Lys aag ccg ctg aag ctc ccg ctg ctg aag cac ctg ccg ccg aag ctc ccg 96 Lys Pro Leu Lys Leu Pro Leu Leu Lys His Leu Pro Pro Lys Leu Pro 20 25 30 cta ctg aag cac ctg ccg ccg aag ctc ccg ctg ctg aag cac ctg ccg 144 Leu Leu Lys His Leu Pro Pro Lys Leu Pro Leu Leu Lys His Leu Pr.o 35 40 45 ccg aag ctc ctg cta ctg aag cac ctg ccg ccg aag ctc ccg ctg ctg 192 Pro Lys Leu Leu Leu Leu Lys His Leu Pro Pro Lys Leu Pro Leu Leu 50 55 60 aag ctg ccg cta ccg aag cac ctg ccg ctg aag ctg ccg cta ccg aag 240 Lys Leu Pro Leu Pro Lys His Leu Pro Leu Lys Leu Pro Leu Pro Lys 65 70 75 80 cac ctg ccg ctg aag ctc ctg ctg ccg aag ctg caa aat aag cat ttt 288 His Leu Pro Leu Lys Leu Leu Leu Pro Lys Leu Gin Asn tys His Phe 85 90 95 ccg ctt gca aaa aag cag gat acg ttc agt atc ctg ctt ttt 330 Pro Leu Ala Lys Lys Gin .Asp Thr Phe Ser Xle Leu Leu Phe 100 105 110

<210> 70 <211> 110 <212> PRT <213> Neisseria meningitidis

129

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<400> 70 Leu Pro Leu 1 Leu Cys 5 Cys Leu Trp Phe Trp Gin Pro Ala Ala Val Lys 10 15 Lys Pro Leu Lys 20 Leu Pro Leu Leu Lys 25 His Leu Pro Pro Lys Leu Pro 30 Leu Leu Lys 35 His Leu Pro Pro Lys Leu 40 Pro Leu Leu Lys His Leu Pro 45 Pro Lys Leu 50 Leu Leu Leu Lys His Leu 55 Pro Pro Lys 60 Leu Pro Leu Leu Lys Leu Pro 65 Leu Pro Lys 70 His Leu Pro Leu Lys Leu 75 Pro Leu Pro Lys 80 His Leu Pro Leu Lys 85 Leu Leu Leu Pro Lys Leu Gin 90 Asn Lys His Phe 95 Pro Leu Ala Lys 100 Lys Gin Asp Thr Phe 105 Ser Ile Leu Leu Phe 110

<210> 71 <211> 2274 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(2274) <400> 71 atg aca aca tta cat ttc tea ggc ttc ccg cgt gtc ggt gcc ttc cgc 48

Met Thr Thr Leu His Phe Ser Gly Phe Pro Arg Val Gly Ala Phe Arg

15 10 15 gaa ttg aaa ttc gca caa gaa aaa tac tgg cgc aaa gaa atc age gag 96

Glu Leu Lys Phe Ala Gin'Glu Lys Tyr Trp Arg Lys Glu Ile Ser Glu

20 25 30 caa gaa ttg ctg get gtt get aaa gac ttg cgc gag aaa aac tgg aaa 144

Gin Glu Leu Leu Ala Val Ala Lys Asp Leu Arg Glu Lys Asn Trp Lys

35 40 45 cac cag gcc get gcc aac gcc gat tac gtt gcc gta ggc gat ttc act 192

His Gin Ala Ala Ala Asn Ala Asp Tyr Val Ala Val Gly Asp Phe Thr

130

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50 55 60 ttc tac gac cac atc ctc gac ctg caa gtc gcc acc ggc gcg att ccc 240 Phe 65 Tyr Asp His Ile Leu 70 Asp Leu Gin Val Ala 75 Thr Gly Ala Ile Pro 80 gcc cgc ttc ggc ttc gac age caa aac cta tet ttg gaa caa ttc ttc 288 Ala Arg Phe Gly Phe 85 Asp Ser Gin Asn Leu 90 Ser Leu Glu Gin Phe 95 Phe caa ctg gcg cgc ggt aac aaa gac caa ttc get atc gaa atg acc aaa 336 Gin Leu Ala Arg 100 Gly Asn Lys Asp Gin 105 Phe Ala Ile Glu Met 110 Thr Lys tgg ttc gac acc aac tac cac tac ttg gtg cct gaa ttc cac gcc gat 384 Trp Phe Asp 115 Thr Asn Tyr His Tyr 120 Leu Val Pro Glu Phe 125 His Ala Asp acc gaa ttt aaa gcc aat gcc aaa cac tac gtt caa cag ctg caa gaa 432 Thr Glu 130 Phe Lys Ala Asn Ala 135 Lys His Tyr Val Gin 140 Gin Leu Gin Glu gcc caa get ttg ggc ctg aaa gcc aaa ccg acc gtt gtg ggt ccg ttg 480 Ala 145 Gin Ala Leu Gly Leu 150 Lys Ala Lys Pro Thr 155 Val Val Gly Pro Leu 160 act ttc ctg tgg gtc ggt aaa gaa aaa ggc gcc gtt gaa ttc gac cgt 528 Thr Phe Leu Trp Val 165 Gly Lys GlU Lys Gly 170 Ala Val Glu Phe Asp 175 Arg ctg age ctg ttg cct aaa ctg ttg cct gtt tac gtt gaa atc ctg act 576 Leu Ser Leu Leu 180 Pro Lys Leu Leu Pro 185 Val Tyr Val Glu Ile 190 Leu Thr get ttg gtt gaa gcc ggt gcc gag tgg att caa atc gac gag cct get 624 Ala Leu Val 195 Glu Ala Gly Ala Glu 200 Trp Ile Gin Ile Asp 205 Glu Pro Ala ttg act gtt gac ctg cct aaa gaa tgg gta gaa gca tac aaa gac gtt 672 Leu Thr 210 Val Asp Leu Pro, .Lys 215 Glu Trp Val Glu Ala 220 Tyr Lys Asp Val tac gcc act ttg age aaa gta agt gcc aaa atc ctg ctg age act tac 720 Tyr 225 Ala Thr Leu Ser Lys 230 Val Ser Ala Lys Ile 235 Leu Leu Ser Thr Tyr 240 ttc ggt tet gtt gcc gaa cac gcc gca ttg ttg aaa tcc ctg cct gtt 768 Phe Gly Ser Val Ala Glu His Ala Ala Leu Leu Lys Ser Leu Pro Val

131

WO 01/85772 PCT/GBO1/02003 245 250 255

gac Asp ggc ctg cac atc gac ttg Leu gta Val cgc gct cct gaa caa ctg gat gcg 816 Gly Leu His Ile 260 Asp Arg 265 Ala Pro Glu Gin Leu 270 Asp Ala ttc gcc gac tac gac aaa gtt ctg tet gcc ggc gtc att gac ggc cgc 864 Phe Ala Asp 275 Tyr Asp Lys Val Leu 280 Ser Ala Gly Val I^e Asp 285 Gly Arg aat att tgg cgc gcc aac ctg aac aaa gtt ttg gaa act gtt gag ctt 912 Asn Ile 290 Trp Arg Ala Asn Leu 295 Asn Lys Val Leu Glu Thr Val 300 Glu Leu ctg caa gcc aaa ctg ggc gac cgt ttg tgg att tcc age tet tgc tcg 960 Leu 305 Gin Ala Lys Leu Gly Asp Arg 310 Leu Trp Ile 315 Ser Ser Ser Cys Ser 320 ctg ctg cac act ccg ttt gac ttg tea gtt gaa gaa aaa ctg aaa gcc 1008 Leu Leu His Thr Pro 325 Phe Asp Leu Ser Val 330 Glu Glu Lys Leu Lys 335 Ala aac aaa ccc gac ctg tac tet tgg ttg gca ttc acc ctg caa aaa acc 1056 Asn Lys Pro Asp Leu 340 Tyr Ser Trp Leu 345 Ala Phe Thr Leu Gin 350 Lys Thr caa gaa ttg cgc gtc ctg aaa gcc gca ttg aac gaa ggc cgt gat tet 1104 Gin Glu Leu 355 Arg Val Leu Lys Ala 360 Ala Leu Asn Glu Gly Arg 365 Asp Ser gtt gcc gaa gaa ctg gct gcc age caa gct gct gcc gac tcc cgt gcc 1152 Val Ala 370 Glu Glu Leu Ala Ala 375 Ser Gin Ala Ala Ala Asp Ser 380 Arg Ala aac age age gaa atc cac cgt gca gac gtt gcc aaa cgc ctg gcc gat 1200 Asn 385 Ser Ser Glu Ile His 390 Arg Ala Asp Val Ala 395 Lys Arg Leu Ala Asp 400 ttg cct gcc aac gca gac caa cgc aaa tcc cca ttt gcc gac cgt atc 1248 Leu Pro Ala Asn Ala 405 Asp -Gin Arg Lys Ser 410 Pro Phe Ala Asp Arg 415 Ile aaa gcg caa caa gca tgg ttg aac ctg cct ctg ctg ccg acg acc aac 1296 Lys Ala Gin Gin Ala 420 Trp Leu Asn Leu 425 Pro Leu Leu Pro Thr 430 Thr Asn atc ggt tet ttc ccg caa act acc gaa atc cgc caa gca cgc gca gcc 1344 Ile Gly ser Phe Pro Gin Thr Thr Glu Ile Arg Gin Ala Arg Ala Ala

132 • · · · · · ···· ···· 9 9 β « 9 9 9 9 9 9 9 • « · · · · · · · « 0 · ··· · · 9 ·

9999 9999 99 999 99 99

WO 01/85772 PCT/GBO1/02003

435 440 445

ttc aaa aaa ggc gaa Phe Lys Lys Gly Glu 450 ctg Leu tet gcc gcc gat tac Tyr gaa gcc gcg atg aaa 1392 Ser Ala 455 Ala Asp Glu 460 Ala Ala Met Lys aaa gaa atc gcc ttg gtg gtt gaa gag caa gaa aaa ctg gac ttg gac 1440 Lys Glu Ile Ala Leu Val Val Glu Glu Gin Glu Lys Leu Asp Leu Asp 465 470 475 480 gta ctg gta cac ggc gaa gcc gag cgt aac gac atg gtt gaa tac ttc 1488 Val Leu val His Gly Glu Ala Glu Arg Asn Asp Met Val Glu Tyr Phe 485 490 495 ggc gaa ttg ttg agc ggt ttt gca ttc acc caa tat ggc tgg gta caa 1536 Gly Glu Leu Leu Ser Gly Phe Ala Phe Thr Gin Tyr Gly Trp Val Gin 500 505 510 agc tac ggt tet cgc tgc gtt aaa cca ccg att atc ttc ggt gac gta 1584 Ser Tyr Gly Ser Arg Cys Val Lys Pro Pro Ile Ile Phe Gly Asp Val 515 520 525 agc cgt cct gaa gcc atg acc gta gct tgg tet act tac gca caa agc 1632 Ser Arg Pro Glu Ala Met Thr Val Ala Trp Ser Thr Tyr Ala Gin Ser 530 535 540 ctg acc aaa cgc ccg atg aaa ggt atg ttg acc ggc cct gta acc att 1680 Leu Thr Lys Arg Pro Met Lys Gly Met Leu Thr Gly Pro Val Thr Ile 545 550 555 560 ctg caa tgg tet ttc gtc cgc aac gat att cct cgc tet acc gtg tgt 1728 Leu Gin Trp Ser Phe Val Arg Asn Asp Ile Pro Arg Ser Thr Val Cys 565 570 575 aaa caa atc gca ctg gct ctg aac gac gaa gta ttg gat ctg gaa aaa 1776 Lys Gin Ile Ala Leu Ala Leu Asn Asp Glu Val Leu Asp Leu Glu Lys 580 585 590 gca ggc atc aaa gtc atc caa atc gac gaa cct gcc atc cgc gaa ggc 1824 Ala Gly Ile Lys Val Ile, .Gin Ile Asp Glu Pro Ala Ile Arg GlU Gly 595 600 605 ctg cct ttg aaa cgt gcc gat tgg gat gcc tac ctg aac tgg gcc ggc 1872 Leu Pro Leu Lys Arg Ala Asp Trp Asp Ala Tyr Leu Asn Trp Ala Gly 610 615 620 gaa tet ttc cgc ctg tcc tet acc ggt tgc gaa gac agc act caa atc 1920 Glu Ser Phe Arg Leu Ser Ser Thr Gly Cys Glu Asp Ser Thr Gin Ile

133

WO 01/85772 PCT/GBO1/02003

625 630 635 640 cac acc cat atg tgc tac tet gag ttc aac gac atc ctg cct gcg att 1968 His Thr His Met Cys Tyr Ser Glu Phe Asn Asp Ile Leu Pro Ala Ile 645 650 655 gct gca atg gat gcg gac gtg atc acc atc gag act tea cgt tcc gac 2016 Ala Ala Met Asp Ala Asp Val Ile Thr Ile Glu Thr Ser Arg Ser Asp 660 665 670 atg gaa ctc ttg acc gcg ttc ggc gaa ttc aaa tac ccg aac gac· atc 2064 Met Glu Leu Leu Thr Ala Phe Gly Glu Phe Lys Tyr Pro Asn Asp Ile 675 680 685 ggc ccg ggg gtt tac gac atc cac agc ccg cgc gta ccg aca gaa gcc 2112 Gly Pro Gly Val Tyr Asp Xle His Ser Pro Arg Val Pro Thr Glu Ala 690 695 700 gaa gtg gag cac ctg ttg cgc aaa gcc atc gag gtt gta ccg gtt gaa 2160 Glu Val Glu His Leu Leu Arg Lys Ala Ile Glu Val Val Pro Val Glu 705 710 715 720 cgt ctg tgg gtt aac ccg gac tgc ggc ctg aaa aca cgc ggc tgg aaa 2208 Arg Leu Trp Val Asn Pro Asp Cys Gly Leu Lys Thr Arg Gly Trp Lys 725 730 735 gaa act ctg gaa caa ctc caa gtg atg atg aac gta acc cac aaa ttg 2256 Glu Thr Leu Glu Gin Leu Gin Val Met Met Asn Val Thr His Lys Leu 740 745 750 cgt gcc gaa ttg gcg aaa 2274 Arg Ala Glu Leu Ala Lys

755 <210> 72 <211> 758 <212> PRT

<213> Neisseria meningitidis <400> 72 Met Thr Thr Leu His Phe Ser Gly Phe Pro Arg Val Gly Ala Phe Arg 1 5 10 15 Glu Leu Lys phe Ala Gin Glu Lys Tyr Trp Arg Lys Glu Ile Ser Glu 20 25 30 Gin Glu Leu Leu Ala Val Ala Lys Asp Leu Arg Glu Lys Asn Trp Lys

134

WO 01/85772

V ·

44·«···» «fc · · 4 ·

4« 4

4 · 44 4

4 · 4 * · ·

4 4 · · · · 4

9 9 9 9 9 9 9

99 ·9 9 99 9 9

PCT/GBO1/02003

His Gin 50 Ala Ala Ala Asn Ala 55 Asp Tyr Val Ala Val 60 Gly Asp Phe Thr Phe 65 Tyr Asp His Ile Leu 70 Asp Leu Gin Val Ala 75 Thr Gly Ala Ile Pro 80 Ala Arg Phe Gly Phe 85 Asp Ser Gin Asn Leu 90 Ser Leu Glu Gin Phe 95 Phe Gin Leu Ala Arg 100 Gly Asn Lys Asp Gin 105 Phe Ala Ile Glu Met 110 Thr Lys Trp Phe Asp 115 Thr Asn Tyr His Tyr 120 Leu Val Pro Glu Phe 125 His Ala Asp Thr Glu 130 Phe Lys Ala Asn Ala 135 Lys His Tyr Val Gin 140 Gin Leu Gin Glu Ala 145 Gin Ala Leu Gly Leu 150 Lys Ala Lys Pro Thr 155 Val Val Gly Pro Leu 160 Thr Phe Leu Trp Val 165 Gly Lys Glu Lys Gly 170 Ala Val Glu Phe Asp 175 Arg Leu Ser Leu Leu 180 Pro Lys Leu Leu Pro 185 Val Tyr Val Glu Ile 190 Leu Thr Ala Leu Val 195 Glu Ala Gly Ala Glu 200 Trp Ile Gin Ile Asp 205 Glu Pro Ala Leu Thr 210 Val Asp Leu Pro Lys 215 Glu Trp Val Glu Ala 220 Tyr Lys Asp Val Tyr 225 Ala Thr Leu Ser Lys 230 Val Ser Ala Lys Ile 235 Leu Leu Ser Thr Tyr 240 Phe Gly Ser Val Ala 245 Glu His Ala Ala Leu 250 Leu Lys Ser Leu Pro 255 Val Asp Gly Leu His 260 Ile Asp Leu val Arg 265 Ala Pro Glu Gin Leu 270 Asp Ala Phe Ala Asp 275 Tyr Asp Lys Val Leu 280 Ser Ala Gly Val Ile 285 Asp Gly Arg Asn Ile Trp Arg Ala Asn Leu Asn Lys Val Leu Glu Thr Val Glu Leu

135 ·«·« • •flfl flflfl· fl fl

WO 01/85772 PCT/GBO1/02003 290 295 300 Leu Gin Ala Lys Leu Gly Asp Arg Leu Trp Ile Ser Ser Ser Cys Ser 305 310 315 320 Leu Leu His Thr Pro Phe Asp Leu Ser Val Glu Glu Lys Leu Lys Ala 325 330 335 Asn Lys Pro Asp Leu Tyr Ser Trp Leu Ala Phe Thr Leu Gin Lys Thr 340 345 350 Gin Glu Leu Arg Val Leu Lys Ala Ala Leu Asn Glu Gly Arg Asp Ser 355 360 365 Val Ala Glu Glu Leu Ala Ala Ser Gin Ala Ala Ala Asp Ser Arg Ala 370 375 ' 380 Asn Ser Ser Glu Ile His Arg Ala Asp Val Ala Lys Arg Leu Ala Asp 385 390 395 400 Leu Pro Ala Asn Ala Asp Gin Arg Lys Ser Pro Phe Ala Asp Arg Ile 405 410 415 Lys Ala Gin Gin Ala Trp Leu Asn Leu Pro Leu Leu Pro Thr Thr Asn 420 425 430 Ile Gly Ser Phe Pro Gin Thr Thr Glu Ile Arg Gin Ala Arg Ala Ala 435 440 445 Phe Lys Lys Gly Glu Leu Ser Ala Ala Asp Tyr Glu Ala Ala Met Lys 450 455 460 Lys Glu Ile Ala Leu Val Val Glu Glu Gin Glu Lys Leu Asp Leu Asp 465 470 475 480 Val Leu Val His Gly Glu Ala Glu Arg Asn Asp Met Val Glu Tyr Phe 485 490 495 Gly Glu Leu Leu Ser Gly Phe Ala Phe Thr Gin Tyr Gly Trp Val Gin 500 505 510 Ser Tyr Gly Ser Arg Cys Val Lys Pro Pro Ile Ile Phe Gly Asp Val 515 520 525 Ser Arg Pro Glu Ala Met Thr Val Ala Trp Ser Thr Tyr Ala Gin Ser 530 535 540 Leu Thr Lys Arg Pro Met Lys Gly Met Leu Thr Gly Pro Val Thr Ile

136 • ·· · · · · ·

WO 01/85772 PCT/GB01/02003

545 550 555 560

Leu Gin Trp Ser Phe 565 Val Arg Asn Asp Ile 570 Pro Arg Ser Thr Val 575 Cys Lys Gin Ile Ala 580 Leu Ala Leu Asn Asp 585 Glu Val Leu Asp Leu 590 Glu Lys Ala Gly Ile 595 Lys Val Ile Gin Ile 600 Asp Glu Pro Ala Ile 605 Arg Glu Gly Leu Pro 610 Leu Lys Arg Ala Asp 615 Trp Asp Ala Tyr Leu 620 Asn Trp Ala Gly Glu 625 Ser Phe Arg Leu Ser 630 Ser Thr Gly Cys Glu 635 Asp Ser Thr Gin Ile 640 His Thr His Met Cys 645 Tyr Ser Glu Phe Asn 650 Asp Ile Leu Pro Ala 655 Ile Ala Ala Met Asp 660 Ala Asp Val Ile Thr 665 Ile Glu Thr Ser Arg 670 Ser Asp Met Glu Leu 675 Leu Thr Ala Phe Gly 680 Glu Phe Lys Tyr Pro 685 Asn Asp Ile Gly Pro 690 Gly Val Tyr Asp Ile 695 His Ser Pro Arg Val 700 Pro Thr Glu Ala Glu 705 Val Glu His Leu Leu 710 Arg Lys Ala Ile Glu 715 Val Val Pro Val Glu 720 Arg Leu Trp Val Asn 725 Pro Asp cys Gly Leu 730 Lys Thr Arg Gly Trp 735 Lys Glu Thr Leu Glu 740 Gin Leu Gin Val Met 745 Met Asn Val Thr His 750 Lys Leu

Arg Ala Glu Leu Ala Lys 755 <210> 73 <211> 2118 <212> DNA <213> Neisseria meningitidis

137

.. .............

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PCT/GB01/02003 <220>

<221> CDS <222> (1)..(2118) <400> 73

atg Met 1 cgc cgt ttt cta ccg atc gca gcc ata tgc gcc gtc gtc ctg ttg 48 Arg Arg Phe Leu Pro Ile 5 Ala Ala Ile 10 Cys Ala Val Val Leu 15 Leu tac gga ctg acg gcg gca acc ggc agc acc agt tcg ctg gcg gat tat 96 Tyr Gly Leu Thr Ala Ala Thr Gly Ser Thr Ser Ser Leu Ala Asp Tyr 20 25 30 ttc tgg tgg att gtt gcg ttc agc gca atg ctg ctg ctg gtg ttg tcc 144 Phe Trp Trp Ile Val Ala Phe Ser Ala Met Leu Leu Leu Val Leu Ser 35 40 45 gcc gtt ttg gca cgt tat gtc ata ttg ctg ttg aaa gac agg cgc gac 192 Ala Val Leu Ala Arg Tyr Val Ile Leu Leu Leu Lys Asp Arg Arg Asp 50 55 60 ggc gta ttc ggt tcg cag att gcc aaa' cgc ctt tcc ggg atg ttt acg 240 Gly Val Phe Gly Ser Gin Ile Ala Lys Arg Leu Ser Gly Met Phe Thr 65 70 75 80 ctg gtt gcc gta ctg ccc ggc gtg ttt ctg ttc ggc gtt tcc gca cag 288 Leu Val Ala Val Leu Pro Gly val Phe Leu Phe Gly Val Ser Ala Gin 85 90 95 ttt atc aac ggc acg att aat tcg tgg ttc ggc aac gat acc cac gag 336 Phe Ile Asn Gly Thr Ile Asn Ser Trp Phe Gly Asn Asp Thr His Glu 100 105 110 gcg ctt gaa cgc agc ctc aat ttg agc aag tcc gca ttg aat ctg gcg 384 Ala Leu Glu Arg Ser Leu Asn Leu Ser Lys Ser Ala Leu Asn Leu Ala 115 120 125 gca gac aac gcc ctt ggc aac gcc atc ccc gtg cag ata gac’ ctc atc 432 Ala Asp Asn Ala Leu Gly Asn Ala Ile Pro val Gin Ile Asp Leu Ile 130 135 140 ggc gcg gct tcc ctg ccc ggg gat atg ggc agg gtg ctg gaa cat tac 480 Gly Ala Ala Ser Leu Pro Gly Asp Met Gly Arg val Leu Glu His Tyr 145 150 155 160 gcc ggc agc ggt ttt gcc cag ctt gcc ctg tac aat gcc gca agc ggc 528 Ala Gly Ser Gly Phe Ala Gin Leu Ala Leu Tyr Asn Ala Ala Ser Gly 165 170 175

138 • ·

WO 01/85772 PCT/GBO1/02003

aaa Lys atc gaa aaa age atc aac ccg cac aag ctc gat cag ccg ttt Pro Phe 190 cca Pro 576 Ile Glu Lys 180 Ser ile Asn Pro His Lys 185 Leu Asp Gin ggt aag gcg cgt tgg gaa aaa atc caa cag gcg ggt tcg gtc agg gat 624 Gly Lys Ala Arg Trp Glu Lys Ile Gin Gin Ala Gly Ser Val Arg Asp 195 200 205 ttg gaa age ata ggc ggc gta ttg tac gcg cag ggc tgg ctg tcg gca 672 Leu Glu Ser Ile Gly Gly Val Leu Tyr Ala Gin Gly Trp Leu Ser Ala 210 215 220 ggt acg cac aac ggg cgc gat tac gcc ttg ttt ttc cgt cag ccg gtt 720 Gly Thr His Asn Gly Arg Asp Tyr Ala Leu Phe Phe Arg Gin Pro Val 225 230 235 240 ccc aaa ggc gtg gca gag gat gcc gtc tta atc gaa aag gca agg gcg 768 Pro Lys Gly Val Ala Glu Asp Ala Val Leu Ile Glu Lys Ala Arg Ala 245 250 255 aaa tat gct gag ttg agt tac age aaa aaa ggt ttg cag acc ttt ttc 816 Lys Tyr Ala Glu Leu Ser Tyr Ser Lys Lys Gly Leu Gin Thr Phe Phe 260 265 270 ctg gca acc ctg ctg att gcc tcg ctg ctg tcg att ttt ctt gca ctg 864 Leu Ala Thr Leu Leu Ile Ala Ser Leu Leu Ser Ile Phe Leu Ala Leu 275 280 285 gtc atg gca ctg tat ttc gcc cgc cgt ttc gtc gaa ccc gtc cta tcg 912 Val Met Ala Leu Tyr Phe Ala Arg Arg Phe Val Glu Pro Val Leu Ser 290 295 300 ctt gcc gag ggg gcg aag gcg gtg gcg caa ggc gat ttc age cag acg 960 Leu Ala Glu Gly Ala Lys Ala Val Ala Gin Gly Asp Phe Ser Gin Thr 305 310 315 320 cgc ccc gtg ttg cgc aac gac gag ttc gga cgc ttg acc aag ttg ttc 1008 Arg Pro Val Leu Arg Asn Asp Glu Phe Gly Arg Leu Thr Lys Leu Phe 325 330 335 aac cac atg acc gag cag ctt tcc atc gcc aaa gaa gca gac gag cgc 1056 Asn His Met Thr Glu Gin Leu Ser Ile Ala Lys Glu Ala Asp Glu Arg 340 345 350 aac cgc cgg cgc gag gaa gcc gcc aga cat tat ctc gaa tgc gtg ttg 1104 Asn Arg Arg Arg Glu Glu Ala Ala Arg His Tyr Leu Glu Cys Val Leu 355 360 365

139

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gag ggg Glu Gly 370 ctg acc acg ggc gtg gtg gtg ttt gac gaa caa ggc tgt ctg 1152 Leu Thr Thr Gly Val 375 Val Val Phe Asp Glu 380 Gin Gly Cys Leu aaa acc ttc aac aaa gcg gcg gaa cag att ttg ggg atg čcg ctt acc 1200 Lys Thr Phe Asn Lys Ala Ala Glu Gin Ile Leu Gly Met Pro Leu Thr 385 390 395 - 400 ccc ctg tgg ggc agc agc cgg cac ggt tgg cac ggc gtt tcg gcg cag 1248 Pro Leu Trp Gly Ser Ser Arg His Gly Trp His Gly Val Ser Ala Gin 405 410 415 cag tcc ctg ctt gcc gaa gtg ttt gcc gcc atc ggc gcg gcg gca ggt 1296 Gin Ser Leu Leu Ala Glu Val Phe Ala Ala Ile Gly Ala Ala Ala Gly 420 425 430 acg gac aaa ccg gtc cat gtg aaa tat gcc gcg ccg gac gat gcc aaa 1344 Thr Asp Lys Pro Val His Val Lys Tyr Ala Ala Pro Asp Asp Ala Lys 435 440 445 atc ctg ctg ggc aag gca acc gtc ctg ccc gaa gac aac ggc aac ggc 1392 Ile Leu Leu Gly Lys Ala Thr Val Leu Pro Glu Asp Asn Gly Asn Gly 450 455 460 gtg gta atg gtg att gac gac atc acc gtt ttg ata cac gcg caa aaa 1440 Val Val Met val Ile Asp Asp Ile Thr Val Leu Ile His Ala Gin Lys 465 470 475 480 gaa gcc gcg tgg ggc gaa gtg gca aaa cgg ctg gca cac gaa atc cgc 1488 Glu Ala Ala Trp Gly Glu Val Ala Lys Arg Leu Ala His Glu Ile Arg 485 490 495 aat ccg ctc acg ccc atc cag ctt tet gcc gaa cgg ctg gcg tgg aaa 1536 Asn Pro Leu Thr Pro Ile Gin Leu Ser Ala Glu Arg Leu Ala Trp Lys 500 505 510 ttg ggc ggg aag ctg gac gag cag gac gcg caa atc ctg aca cgt tcg 1584 Leu Gly Gly Lys Leu Asp Glu Gin Asp Ala Gin Ile Leu Thr Arg Ser 515 • 520 525 acc gac acc atc atc aaa caá gtg gcg gca tta aaa gaa atg gtc gag 1632 Thr Asp Thr Ile Ile Lys Gin Val Ala Ala Leu Lys Glu Met Val Glu 530 535 540 gca ttc cgc aat tac gcg cgt tcc cct tcg ctc aaa ttg gaa aat cag 1680 Ala Phe Arg Asn Tyr Ala Arg Ser Pro Ser Leu Lys Leu Glu Asn Gin 545 550 555 560

140

WO 01/85772 PCT/GB01/02003

gat Asp ttg aac gcc Ala tta atc ggc gat gtg ttg gca ttg tac gaa gct ggt Gly 1728 Leu Asn Leu 565 Ile Gly Asp Val Leu 570 Ala Leu Tyr Glu Ala 575 ccg tgc cgg ttt gcg gcg gaa ctt gcc ggc gaa ccg ctg atg atg gcg 1776 Pro Cys Arg Phe 580 Ala Ala Glu Leu Ala 585 Gly Glu Pro Leu Met 590 Met Ala gcg gat acg acc gcc atg cgg cag gtg ctg cac aat att ttc aaa aat 1824 Ala Asp Thr 595 Thr Ala Met Arg Gin Val 600 Leu His Asn Ile 605 Phe Lys Asn gcc gcc gaa gcg gcg gaa gaa gcc gat gtg ccc gaa gtc agg gta aaa 1872 Ala Ala 610 Glu Ala Ala Glu Glu Ala Asp 615 Val Pro Glu Val 620 Arg Val Lys tcg gaa gcg ggg cag gac gga cgg att gtc ctg aca gtt tgc gac aac 1920 Ser 625 Glu Ala Gly Gin Asp 630 Gly Arg Ile Val Leu 635 Thr Val Cys Asp Asn 640 ggc aag ggg ttc ggc agg gaa atg ctg cac aat gcc ttc gag ccg tat 1968 Gly Lys Gly Phe Gly Arg 645 Glu Met Leu His 650 Asn Ala Phe Glu Pro 655 Tyr gta acg gac aaa ccg gct gga acg gga ttg gga ctg ccc gtg gtg aaa 2016 Val Thr Asp Lys 660 Pro Ala Gly Thr Gly 665 Leu Gly Leu Pro Val 670 Val Lys aaa atc att gaa gaa cac ggc ggc cgc atc agc ctg agc aat cag gat 2064 Lys Ile Ile 675 Glu Glu His Gly Gly Arg 680 Ile Ser Leu Ser 685 Asn Gin Asp gcg ggc ggc gcg tgt gtc aga atc atc ttg cca aaa acg gta gaa act 2112 Ala Gly 690 Gly Ala Cys Val Arg Ile Ile 695 Leu Pro Lys Thr 700 Val Glu Thr

tat gcg 2118

Tyr Ala

705 <210> 74 <211> 706 <212> PRT <213> Neisseria meningitidis <400 74

141

WO 01/85772

PCT/GB01/02003

Met 1 Arg Arg Phe Leu 5 Pro Ile Ala Ala Ile 10 Cys Ala Val Val Leu 15 Leu Tyr Gly Leu Thr 20 Ala Ala Thr Gly Ser 25 Thr Ser Ser Leu Ala 30 Asp Tyr Phe Trp Trp 35 Ile Val Ala Phe Ser 40 Ala Met Leu Leu Leu 4.5 Val Leu Ser Ala Val 50 Leu Ala Arg Tyr Val 55 Ile Leu Leu Leu Lys 60 Asp Arg Arg Asp Gly 65 Val Phe Gly Ser Gin 70 Ile Ala Lys Arg Leu 75 Ser Gly Met Phe Thr 80 Leu Val Ala Val Leu 85 Pro Gly Val Phe Leu 90 Phe Gly Val Ser Ala 95 Gin Phe Ile Asn Gly 100 Thr Ile Asn Ser Trp 105 Phe Gly Asn Asp Thr 110 His Glu Ala Leu Glu 115 Arg Ser Leu Asn Leu 120 Ser Lys Ser Ala Leu 125 Asn Leu Ala Ala Asp 130 Asn Ala Leu Gly Asn 135 Ala Ile Pro Val Gin 140 Ile Asp Leu Ile Gly 145 Ala Ala Ser Leu Pro 150 Gly Asp Met Gly Arg 155 Val Leu Glu His Tyr 160 Ala Gly Ser Gly Phe 165 Ala Gin Leu Ala Leu 170 Tyr Asn Ala Ala Ser 175 Gly Lys Ile Glu Lys 180 Ser Ile Asn Pro His 185 Lys Leu Asp Gin Pro 190 Phe Pro Gly Lys Ala 195 Arg Trp Glu Lys Ile 200 Gin Gin Ala Gly Ser 205 Val Arg Asp Leu Glu 210 Ser Ile Gly Gly Val 215 Leu Tyr Ala Gin Gly 220 Trp Leu Ser Ala Gly 225 Thr His Asn Gly Arg 230 Asp Tyr Ala Leu Phe 235 Phe Arg Gin Pro Val 240 Pro Lys Gly Val Ala Glu Asp Ala Val Leu Ile Glu Lys Ala Arg Ala

245 250 255

142

WO 01/85772

PCT/GB01/02003

Lys Tyr Ala Glu 260 Leu Ser Tyr Ser Lys 265 Lys Gly Leu Gin Thr 270 Phe Phe Leu Ala Thr 275 Leu Leu Ile Ala Ser 280 Leu Leu Ser Ile Phe 285 Leu Ala Leu Val Met 290 Ala Leu Tyr Phe Ala 295 Arg Arg Phe Val Glu 300 Pro Val Leu Ser Leu 305 Ala Glu Gly Ala Lys 310 Ala Val Ala Gin Gly Asp 315 Phe Ser Gin Thr 320 Arg Pro Val Leu Arg 325 Asn Asp Glu Phe Gly 330 Arg Leu Thr Lys Leu 335 Phe Asn His Met Thr 340 Glu Gin Leu S<sr i Ile ,345 Ala Lys Glu Ala Asp 350 Glu Arg Asn Arg Arg Arg 355 Glu Glu Ala Ala 360 Arg His ' 1 Tyr Leu Glu 365 Cys Val Leu Glu Gly 370 Leu Thr Thr Gly Val 375 Val Val Phe Asp Glu 380 Gin Gly Cys Leu Lys 385 Thr Phe Asn Lys Ala 390 Ala Glu Gin Ile Leu 395 Gly Met Pro Leu Thr 400 Pro Leu Trp Gly Ser 405 Ser Arg His Gly Trp 410 His Gly Val Ser Ala 415 Gin Gin Ser Leu Leu 420 Ala Glu Val Phe Ala 425 Ala Ile Gly Ala Ala 430 Ala Gly Thr Asp Lys 435 Pro Val His Val Lys 440 Tyr Ala Ala Pro Asp 445 Asp Ala Lys Ile Leu 450 Leu Gly Lys Ala Thr 455 Val Leu Pro Glu Asp 460 Asn Gly Asn Gly Val 465 Val Met Val Ile Asp _x Asp 470 Ile Thr Val Leu 475 Ile His Ala Gin Lys 480 Glu Ala Ala Trp Gly 485 Glu Val Ala Lys Arg 490 Leu Ala His Glu Ile 495 Arg Asn Pro Leu Thr 500 Pro Ile Gin Leu Ser 505 Ala Glu Arg Leu Ala 510 Trp Lys

143

WO 01/85772 PCT/GB01/02003

Leu Gly Gly 515 Lys Leu Asp Glu Gin 520 Asp Ala Gin Ile Leu 525 Thr Arg Ser Thr Asp 530 Thr Ile Ile Lys Gin 535 Val Ala Ala Leu Lys 540 Glu Met Val Glu Ala 545 Phe Arg Asn Tyr Ala 550 Arg Ser Pro Ser Leu 555 Lys Leu Glu Asn Gin 560 Asp Leu Asn Ala Leu 565 Ile Gly Asp Val Leu 570 Ala Leu Tyr Glu Ala 575 Gly Pro Cys Arg Phe 580 Ala Ala Glu Leu Ala 585 Gly Glu Pro Leu Met 590 Met Ala Ala Asp Thr 595 Thr Ala Met Arg Gin 600 Val Leu His Asn Ile 605 Phe Lys Asn Ala Ala 610 Glu Ala Ala Glu Glu 615 Ala Asp Val Pro Glu 620 Val Arg Val Lys Ser 625 Glu Ala Gly Gin Asp 630 Gly Arg Ile Val Leu 635 Thr Val Cys Asp Asn 640 Gly Lys Gly Phe Gly Arg 645 Glu Met Leu His 650 Asn Ala Phe Glu Pro 655 Tyr Val Thr Asp Lys 660 Pro Ala Gly Thr Gly 665 Leu Gly Leu Pro Val 670 Val Lys Lys Ile Ile 675 Glu Glu His Gly Gly 680 Arg Ile Ser Leu Ser 685 Asn Gin Asp Ala Gly Gly Ala Cys Val Arg Ile Ile Leu Pro Lys Thr Val Glu Thr

690 695 700

Tyr Ala 705 <210> 75 <211> 1686 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS

144 • 9

WO 01/85772 PCT/GB01/02003 <222> (1)..(1686) <400> 75

atg tta caa tcc gaa aat tcg aaa aat tta atc tet tgt tca ata aag Lys 48 Met 1 Leu Gin Ser Glu 5 Asn Ser Lys Asn Leu 10 Ile Ser Cys Ser Ile 15 gct tta cca atc atg att tet acc aac ggc atc acc atg cag ttc ggc 96 Ala Leu Pro Ile Met Ile Ser Thr Asn Gly Ile Thr Met Gin Phe Gly 20 25 30 gcg aag ccg ctg ttt gaa aac gta tcc gtt aaa ttc ggc gaa ggc aac 144 Ala Lys Pro Leu Phe Glu Asn Val Ser Val Lys Phe Gly Glu Gly Asn 35 40 45 cgc tac ggt ttg atc ggc gcg aac ggc tca ggc aaa tcc acc ttc atg 192 Arg Tyr Gly Leu Ile Gly Ala Asn Gly Ser Gly Lys Ser Thr Phe Met 50 55 60 aaa atc ctc ggc ggc gat ttg gaa cag aca gcc ggc gaa gtg gcg att 240 Lys Ile Leu Gly Gly Asp Leu Glu Gin Thr Ala Gly Glu Val Ala Ile 65 70 75 80 gaa aac ggc gtg cgt ttg ggt aaa ttg cgc caa gac cag ttt gcc tac 288 Glu Asn Gly Val Arg Leu Gly Lys Leu Arg Gin Asp Gin Phe Ala Tyr 85 90 95 gaa gat atg cgc gtg ctg gac gtg gta atg atg ggt cac acc gaa atg 336 Glu Asp Met Arg Val Leu Asp Val Val Met Met Gly His Thr Glu Met 100 105 110 tgg gcg gcg atg acc gaa cgt gat gcg att tac gcc aat ccc gaa gcc 384 Trp Ala Ala Met Thr Glu Arg Asp Ala Ile Tyr Ala Asn Pro Glu Ala 115 120 125 acc gaa gac gac tac atg aaa gcc gca gaa ctg gaa gcc aag ttc gcc 432 Thr Glu Asp Asp Tyr Met Lys Ala Ala GlU Leu Glu Ala Lys Phe Ala 130 135 140 gaa tac gac ggc tac acc, gcc gaa gcg cgt gcc gcc gag ttg ttg agc 480 Glu Tyr Asp Gly Tyr Thr Ala Glu Ala Arg Ala Ala GlU Leu Leu Ser 145 150 155 160 ggc gtg ggc att tcc gaa gat ttg cac aat gca acc atg gca gaa gtt 528 Gly val Gly Ile Ser Glu Asp Leu His Asn Ala Thr Met Ala Glu Val 165 170 175 gcc ccg ggc ttc aaa ctg cgc gta ttg ctg gcg caa gcc ctg ttc tcc 576

145

WO 01/85772

PCT/GB01/02003

Ala Pro Gly Phe 180 Lys Leu Arg Val Leu 185 Leu Ala Gin Ala Leu 190 Phe Ser aag ccg gat gta ttg ctc ttg gac gaa ccg acc aat aac ttg gac att 624 Lys Pro Asp 195 Val Leu Leu Leu Asp 200 Glu Pro Thr Asn Asn 205 Leu Asp Ile aat acc atc cgt tgg ttg gaa ggc gtg ttg aac cag tac gac tcc acg 672 Asn Thr 210 Ile Arg Trp Leu Glu 215 Gly Val Leu Asn Gin 220 Tyr Asp Ser Thr atg att atc atc tcg cac gac cgc cac ttc ttg aac gaa gtc tgc acg 720 Met 225 Ile Ile Ile Ser His 230 Asp Arg His Phe Leu 235 Asn Glu Val Cys Thr 240 cat atg gca gat ttg gat tac aác acc atc acc atc tat ccg ggc aac 7S8 His Met Ala Asp Leu 245 Asp Tyr Asn Thr Ile 250 Thr Ile Tyr Pro Gly 255 Asn tac gac gac tat atg ctc gcc tcc gcc caa tcg cgc gaa cgc gcc ctg 816 Tyr Asp Asp Tyr 260 Met Leu Ala Ser Ala 265 Gin Ser Arg Glu Arg 270 Ala Leu aaa gac aat gcc aag gcg aag gaa aaa ttg cag gaa ttg caa gaa ttt 864 Lys Asp Asn 275 Ala Lys Ala Lys Glu 280 Lys Leu Gin Glu Leu 285 Gin Glu Phe gtg gcg cgg ttc agt gcc aac aaa tcc aaa gcc cgt cag gca acc age 912 Val Ala 290 Arg Phe Ser Ala Asn 295 Lys Ser Lys Ala Arg 300 Gin Ala Thr Ser cgt ctg aaa cag gca gac aaa atc aaa tcg gag atg gtc gag gtc aaa 960 Arg 305 Leu Lys Gin Ala Asp 310 Lys Ile Lys Ser Glu 315 Met Val Glu Val Lys 320 ccc tet acc cgt caa aac ccg tat atc cgt ttt gaa gcc gat gaa aaa 1008 Pro Ser Thr Arg Gin 325 Asn Pro Tyr Ile Arg 330 Phe Glu Ala Asp Glu 335 Lys gcc aag ctg cac cgt cag 'get gtg gaa gtt gaa aaa ctg gcg aaa cgc 1056 Ala Lys Leu His 340 Arg Gin Ala Val Glu 345 Val Glu Lys Leu Ala 350 Lys Arg ttt gaa acc cag ttg ttt aaa aac ctg aac ttt atc ctt gaa gcg gga 1104 Phe Glu Thr 355 Gin Leu Phe Lys Asn 360 Leu Asn Phe Ile Leu 365 Glu Ala Gly caa cgc ctc gcc atc atc ggc ccg aac ggc gcg ggc aaa tcc acc ctg 1152

146

WO 01/85772

PCT/GBO1/02003

Gin Arg 370 Leu Ala Ile Ile Gly 375 Pro Asn Gly Ala Gly Lys 380 Ser Thr Leu ctg aaa ctc ttg gcc ggc gcg tac aac ccc gaa tat tea gac ggt ctg 1200 Leu 385 Lys Leu Leu Ala Gly 390 Ala Tyr Asn Pro Glu 395 Tyr Ser Asp Gly Leu 400 ctg ccg gac gaa ggc age atc aaa tgg gcg gaa aaa gcc age gtc ggc 1248 Leu Pro Asp Glu Gly 405 Ser Ile Lys Trp Ala 410 Glu Lys Alá Ser Val 415 Gly tac tat ccg caa gat cac gaa aac gac ttc gat gtc gat atg gat ttg 1296 Tyr Tyr Pro Gin 420 Asp His Glu Asn Asp 425 Phe Asp val Asp Met 430 Asp Leu age gaa tgg atg cgc caa tgg ggg cag gac ggc gac gac gaa caa gtc 1344 Ser GlU Trp 435 Met Arg Gin Trp Gly 440 Gin Asp Gly Asp Asp 445 Glu Gin Val atc cgc ggc act ttg ggg cgt ttg ctc ttc ggc agt aac gat gtc gtg 1392 Ile Arg 450 Gly Thr Leu Gly Arg 455 Leu Leu Phe Gly Ser 460 Asn Asp Val Val aaa aaa gtg aag gtt ctc tcc ggt ggt gaa aaa ggc cgt atg ctt tac 1440 Lys 465 Lys Val Lys val Leu 470 Ser Gly Gly Glu Lys 475 Gly Arg Met Leu Tyr 480 ggc aaa ctg ttg ctg ttg aaa ccc aat gtc tta gtc atg gat gaa ccg 1488 Gly Lys Leu Leu Leu 485 Leu Lys Pro Asn Val 490 Leu Val Met Asp Glu 495 Pro acc aac cac atg gac atg gaa age atc gaa tcc ttg aac atg gcg ctg 1536 Thr Asn His Met 500 Asp Met Glu Ser Ile 505 Glu Ser Leu Asn Met 510 Ala Leu gaa aaa tac aac ggc acg ctg att ttc gtc tcc cac gac cgt cag ttc 1584 Glu Lys Tyr 515 Asn Gly Thr Leu Ile 520 Phe Val Ser His Asp 525 Arg Gin Phe gtt tet tcc ctg gct acc ,caa atc att gaa ttg gac ggc aaa ggc gga 1632 Val Ser 530 Ser Leu Ala Thr Gin 535 Ile Ile Glu Leu Asp 540 Gly Lys Gly Gly tat gaa cac tac ttg ggc gat tac gaa age tat ctc gag aaa aaa ggc 1680 Tyr 545 Glu His Tyr Leu Gly Asp 550 Tyr Glu Ser Tyr 555 Leu Glu Lys Lys Gly 560

gta gca

1686

147 ·· ·· ·· · ·· ···· ··· · ···· · · · • · · · · · · ·

WO 01/85772 PCT/GBO ί/02003

Val Ala <210> 76 <211> 562 <212> PRT <213> Neisseria meningitidis <400> 76

Met 1 Leu Gin Ser Glu 5 Asn Ser Lys Asn Leu 10 Ile Ser Cys Ser Ile 15 Lys Ala Leu Pro Ile 20 Met Ile Ser Thr Asn 25 Gly Ile Thr Met Gin 30 Phe Gly Ala Lys Pro 35 Leu Phe Glu Asn Val 40 Ser Val Lys Phe Gly 45 Glu Gly Asn Arg Tyr 50 Gly Leu Ile Gly Ala 55 Asn Gly Ser Gly Lys 60 Ser Thr Phe Met Lys 65 Ile Leu Gly Gly Asp 70 Leu Glu Gin Thr Ala 75 Gly Glu Val Ala Ile 80 Glu Asn Gly Val Arg 85 Leu Gly Lys Leu Arg 90 Gin Asp Gin Phe Ala 95 Tyr Glu Asp Met Arg 100 Val Leu Asp Val Val 105 Met Met Gly His Thr 110 Glu Met Trp Ala Ala 115 Met Thr Glu Arg Asp 120 Ala Ile Tyr Ala Asn 125 Pro Glu Ala Thr Glu 130 Asp Asp Tyr Met Lys 135 Ala Ala Glu Leu Glu 140 Ala Lys Phe Ala Glu 145 Tyr Asp Gly Tyr Thr 150 Ala Glu Ala Arg Ala 155 Ala Glu Leu Leu Ser 160 Gly Val Gly Xle Ser 165 Glu 'Asp Leu His Asn 170 Ala Thr Met Ala Glu 175 Val Ala Pro Gly Phe 180 Lys Leu Arg Val Leu 185 Leu Ala Gin Ala Leu 190 Phe Ser Lys Pro Asp 195 Val Leu Leu Leu Asp 200 Glu Pro Thr Asn Asn 205 Leu Asp Ile

148 • «

WO 01/85772 PCT/GB01/02003 Asn Thr Ile Arg Trp Leu Glu Gly 210 215 Val Leu Asn Gin Tyr Asp' Ser Thr 220 Met Ile Ile Ile Ser His Asp Arg 225 230 His Phe Leu Asn Glu Val Cys Thr 235 240 His Met Ala Asp Leu Asp Tyr Asn 245 Thr Ile Thr Ile Tyr Pro Gly Asn 250 255 Tyr Asp Asp Tyr Met Leu Ala Ser 260 Ala Gin Ser Arg Glu Arg Ala Leu 265 270 Lys Asp Asn Ala Lys Ala Lys Glu 275 280 Lys Leu Gin Glu Leu Gin Glu Phe 285 Val Ala Arg Phe Ser Ala Asn Lys 290 295 Ser Lys Ala Arg Gin Ala Thr Ser 300 Arg Leu Lys Gin Ala Asp Lys Ile 305 310 Lys Ser Glu Met Val Glu Val Lys 315 320 Pro Ser Thr Arg Gin Asn Pro Tyr 325 Ile Arg Phe Glu Ala Asp Glu Lys 330 335 Ala Lys Leu His Arg Gin Ala Val 340 Glu Val Glu Lys Leu Ala Lys Arg 345 350 Phe Glu Thr Gin Leu Phe Lys Asn 355 360 Leu Asn Phe Ile Leu Glu Ala Gly 365 Gin Arg Leu Ala Ile Ile Gly Pro 370 375 Asn Gly Ala Gly Lys Ser Thr Leu 380 Leu Lys Leu Leu Ala Gly Ala Tyr 385 390 Asn Pro Glu Tyr Ser Asp Gly Leu 395 400 Leu Pro Asp Glu Gly Ser Ile Lys 405 Trp Ala Glu Lys Ala Ser Val Gly 410 415 Tyr Tyr Pro Gin Asp His Glu Asn 420 Asp Phe Asp Val Asp Met Asp Leu 425 430 Ser Glu Trp Met Arg Gin Trp Gly 435 440 Gin Asp Gly Asp Asp Glu Gin Val 445 Ile Arg Gly Thr Leu Gly Arg Leu 450 455 Leu Phe Gly Ser Asn Asp Val Val 460

149 *· ···· « · · • · · • · · · • · · « ·· ·· ·· ·· • · · ·

WO 01/85772 PCT/GBO1/02003 Lys 465 Lys Val Lys Val Leu 470 Ser Gly Gly Glu Lys 475 Gly Arg Met Leu Tyr 480 Gly Lys Leu Leu Leu 485 Leu Lys Pro Asn Val 490 Leu Val Met Asp Glu 495 Pro Thr Asn His Met 500 Asp Met Glu Ser Ile 505 Glu Ser Leu Asn Met 510 Ala Leu Glu Lys Tyr 515 Asn Gly Thr Leu Ile 520 Phe Val Ser His Asp 525 Arg Gin Phe val Ser 530 Ser Leu Ala Thr Gin 535 Ile Ile Glu Leu Asp 540 Gly Lys Gly Gly Tyr 545 Glu His Tyr Leu Gly Asp 550 Tyr Glu Ser Tyr 555 Leu Glu Lys Lys Gly 560

Val Ala <210> 77 <211> 1773 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1).. (1773) <400> 77

atg Met 1 agt Ser atc gtg ctg Leu 5 cac ggc His Gly gtg Val gcg gcg ggc aaa ggc att gcc gtc Ile Ala Val 15 48 Ile Val Ala Ala 10 Gly Lys Gly ggt tgc gcc cac ctg att gcg cgc ggt acg gag gaa gtg ccg cag tat 96 Gly Cys Ala His Leu Xle Ala Arg Gly Thr Glu Glu Val Pro Gin Tyr 20 25 30 gat gtt gcg gag gcg gac acc gat gcc gaa gcc gaa cgt ttc gat gcc 144 Asp Val Ala Glu Ala Asp Thr Asp Ala Glu Ala Glu Arg Phe Asp Ala 35 40 45 gcc gtc aaa gcc acg cgc aaa gag ttg gaa cag ctc cgc age gcg att 192 Ala Val Lys Ala Thr Arg Lys Glu Leu Glu Gin Leu Arg Ser Ala Ile

50 55 60

150

49 4499

44 ·· ·· • · 4 » · · · • · · · • 9 4 · · • · * · 944 · · · · * *· · • « * • · ·

4 4 4 4

4 4 9 9

44 49

WO 01/85772

PCT/GB01/02003

ccc gaa aac gcc Ala ccg Pro acc gag ttg ggc gcg ttc atc tcg ctg cac ctg 240 Pro Glu 65 Asn Thr Glu Leu 70 Gly Ala Phe 75 Ile Ser Leu His Leu 80 atg ctc ttg acc gat gtt acc ttg tcg cgc gaa ccc gtc gat att tta 288 Met Leu Leu Thr Asp Val Thr Leu Ser Arg Glu Pro Val Asp Ile Leu 85 90 95 agg gaa caa aaa atc aac gcc gag tgg gca ttg aag cag cag agc gac 336 Arg Glu Gin Lys Ile Asn Ala Glu Trp Ala Leu Lys Gin Gin Ser Asp 100 105 110 aaa ctc gcc gcc caa ttc gac aat atg gac gat gcc tat ttg cgc gaa 384 Lys Leu Ala Ala Gin Phe Asp Asn Met Asp Asp Ala Ty_X Leu Arg Glu 115 120 125 cgc aag cag gat atg ctg caa gtc gtc cgc cgc atc cac aac aac ctg 432 Arg Lys Gin Asp Met Leu Gin Val Val Arg Arg Ile His Asn Asn Leu 130 135 140 atc ggg cag ggc aac gag ttg gaa gtt gcc gac aac ctg ttt gac gaa 480 Ile Gly Gin Gly Asn Glu Leu Glu Val Ala Asp Asn Leu Phe Asp Glu 145 150 155 160 acc gtt ctg att gca aac gac ctt tcg ccc gcc gac acg gtt ttg ttt 528 Thr Val Leu Ile Ala Asn Asp Leu Ser Pro Ala Asp Thr Val Leu Phe 165 170 175 aaa gag cag cgc att gcc gcc ttc gtt acc gat gcc ggc ggc ccc acc 576 Lys Glu Gin Arg Ile Ala Ala Phe Val Thr Asp Ala Gly Gly Pro Thr 180 185 190 ggg cat acg gcg att ttg ggc agg agc ttg gac atc ccg tcc gtc gtc 624 Gly His Thr Ala Ile Leu Gly Arg Ser Leu Asp Ile Pro Ser Val Val 195 200 205 ggg ctg cac aac gcg cgc aaa ctg att acc gag ggc gaa acg gtc att 672 Gly Leu His Asn Ala Arg Lys Leu Ile Thr Glu Gly Glu Thr val Ile 210 -215 220 gtg gac ggt atc aac ggc gtg ttg att atc gcg ccg gat gag tcg gtg 720 Val Asp Gly Ile Asn Gly Val Leu Ile Ile Ala Pro Asp Glu Ser Val 225 230 235 240 ttg aac gaa tac cgc cgc cgt gcc cgc gaa tac cgc agc cac aaa cgc 768 Leu Asn Glu Tyr Arg Arg Arg Ala Arg Glu Tyr Arg Ser His Lys Arg

245 250 255

151 ···· ····

WO 01/85772

PCT/GB01/02003

gat ttg aac aag ctc aaa aaa acc gcc Asp Leu Asn Lys 260 Leu Lys Lys Thr Ala 265 tgc atc gag ctt gtg ggc aat ata gaa Cys Ile Glu 275 Leu Val Gly Asn Ile 280 Glu ctg cac aac ctc ggc gca gac ggc atc Leu His 290 Asn Leu Gly Ala Asp 295 Gly Ile ctt tac ctg aac cgc gat acg atg ccg Leu 305 Tyr Leu Asn Arg Asp 310 Thr Met Pro gtg tac age gcg att gtc aaa aaa atg Val Tyr Ser Ala Ile 325 Val Lys Lys Met cgg aca gtc gat ttg ggt gtg gac aaa Arg Thr Val Asp 340 Leu Gly Val Asp Lys 345 aac age acg ccc aac ggc age ctc aac Asn Ser Thr 355 Pro Asn Gly Ser Leu 360 Asn atc cgc ctg tgc ctt gcc gaa ccg gtc Ile Arg 370 Leu Cys Leu Ala Glu 375 Pro Val gcc atc ctc cgc gcc gcc gcc cac ggc Ala 385 Ile Leu Arg Ala Ala 390 Ala His Gly atg att acc tcc gta tcc gaa gtg cgc Met Ile Thr Ser Val 405 Ser Glu Val Arg acc gcg caa cgc cag ctt gcc gaa cgc Thr Ala Gin Arg 420 Gin Leu Ala Glu Arg 425 ggc atc ggc tgt atg att gaa att ccg Gly Ile Gly 435 Cys Met Ile Glu Ile 440 Pro

gcc gcc acc gct gac ggg gtc 816 Ala Ala Thr Ala Asp 270 Gly Val tcc gcc gaa gac gtg aaa ccg 864 Ser Ala Glu Asp 285 Val Lys Pro ggg ctg ttc cgc age gag ttt 912 Gly Leu Phe 300 Arg Ser Glu Phe tet gaa gac gag cag tac gaa 960 Ser Glu 315 Asp Glu Gin Tyr Glu 320 aaa ggc aaa age gta acg ata 1008 Lys 330 Gly Lys Ser Val Thr 335 Ile aac ccg cgc tgg ttc ggg aaa 1056 Asn Pro Arg Trp Phe 350 Gly Lys ccc gcg ctg ggc atg acc ggc 1104 Pro Ala Leu Gly 365 Met Thr Gly atg ttc cgc acc cag atg cgc 1152 Met Phe Arg 380 Thr Gin Met Arg ccc gtg cgg atg atg tgg ccg 1200 Pro Val 395 Arg Met Met Trp Pro 400 cag tgc ctc atc cac ctc gac 1248 Gin 410 cys Leu Ile His Leu 415 Asp ggc gat gcc ttc ggt aaa gtc 1296 Gly Asp Ala Phe Gly 430 Lys Val tet gcc gcg ctg acc gtc ggc 1344 Šer Ala Ala Leu Thr Val Gly

445

152 ········ · ·

WO 01/85772

PCT/GB01/02003

agt att Ser Ile ttg aaa ctg Leu gtc gat ttc atc tcc gtc ggt acc aac gac ctg 1392 Leu Lys Val Asp Phe 455 Ile Ser Val Gly Thr Asn 460 Asp Leu 450 att caa tac atc ttg tcc gtc gat cgc ggc gac gac agc gtc agc cac 1440 Ile Gin Tyr Ile Leu Ser Val Asp Arg Gly Asp Asp Ser Val Ser His 465 470 475 480 ctc tac cag ccc ggc cat ccc gcc gtg ctg aaa atg ctg caa cac gtc 1488 Leu Tyr Gin Pro Gly His Pro Ala Val Leu Lys Met Leu Gin His Val 485 490 495 atc cgt acc gcc aac cgc atg gac aaa gac gta tcc gta tgc ggc gag 1536 Ile Arg Thr Ala Asn Arg Met Asp Lys Asp Val Ser Val Cys Gly Glu 500 505 510 atg gcg ggc gat acc gcg ttt acc cgc gtt tta ttg ggt atg ggg ctg 1584 Met Ala Gly Asp Thr Ala Phe Thr Arg Val Leu Leu Gly Met Gly Leu 515 520 525 cgc cgt ttt tcc atg aac ccc aac aac atc ctg ccc gtc aaa aac atc 1632 Arg Arg Phe Ser Met Asn Pro Asn Asn Ile Leu Pro Val Lys Asn Ile 530 535 540 att ctg cac agc aat gtc gga cag ctc gaa agc gat att gtg aaa gtc 1680 Ile Leu His Ser Asn Val Gly Gin Leu Glu Ser Asp Ile Val Lys Val 545 550 555 560 atc cgc tgc gaa gac gaa gag aag tcg gaa aag ctg atc aaa cag atg 1728 Ile Arg Cys Glu Asp Glu Glu Lys Ser Glu Lys Leu Ile Lys Gin' Met 565 570 575 aac agc gtg tet gtc gag gaa gaa gcc gac ttc aag ggg cgg aaa 1773 Asn Ser Val Ser Val Glu Glu Glu Ala Asp Phe Lys Gly Arg Lys 580 585 590

<210> 78 <211> 591 <212> PRT <213> Neisseria meningitidis <400> 78

Met Ser Ile Val Leu His Gly Val Ala Ala Gly Lys Gly Ile Ala Val

1 5 10 15

Gly Cys Ala His Leu Ile Ala Arg Gly Thr Glu Glu Val Pro Gin Tyr

153

WO 01/85772

PCT/GB01/02003

Asp Val Ala 35 Glu Ala Asp Thr Asp 40 Ala Glu Ala Glu Arg 45 Phe Asp Ala Ala Val Lys Ala Thr Arg Lys Glu Leu Glu Gin Leu Arg Ser Ala Ile 50 55 60 Pro Glu Asn Ala Pro Thr Glu Leu Gly Ala Phe Ile Ser Leu His. Leu 65 70 75 80 Met Leu Leu Thr Asp Val Thr Leu Ser Arg Glu Pro Val Asp Ile Leu 85 90 95 Arg Glu Gin Lys Ile Asn Ala Glu Trp Ala Leu Lys Gin Gin Ser Asp 100 105 110 Lys Leu Ala Ala Gin Phe Asp Asn Met Asp Asp Ala Tyr Leu Arg Glu 115 120 125 Arg Lys Gin Asp Met Leu Gin Val Val Arg Arg Ile His Asn Asn Leu 130 135 140 Ile Gly Gin Gly Asn Glu Leu Glu Val Ala Asp Asn Leu Phe Asp Glu 145 150 155 160 Thr Val Leu Ile Ala Asn Asp Leu Ser Pro Ala Asp Thr Val Leu Phe 165 170 175 Lys Glu Gin Arg Ile Ala Ala Phe Val Thr Asp Ala Gly Gly Pro Thr 180 185 190 Gly His Thr Ala Ile Leu Gly Arg Ser Leu Asp Ile Pro Ser Val Val 195 200 205 Gly Leu His Asn Ala Arg Lys Leu Ile Thr Glu Gly Glu Thr Val Ile 210 215 220 Val Asp Gly Ile Asn Gly Val Leu Ile Ile Ala Pro Asp Glu Ser Val 225 230 235 240 Leu Asn Glu Tyr Arg Arg Arg Ala Arg Glu Tyr Arg Ser His Lys Arg 245 250 255 Asp Leu Asn Lys Leu Lys Lys Thr Ala Ala Ala Thr Ala Asp Gly Val 260 265 270 Cys Ile Glu Leu Val Gly Asn Ile Glu Ser Ala Glu Asp Val Lys Pro

154

• « • • · » fc • · • • » · · · • • • • · • fc • · • · • · • · • • · • • • · « • · · • · • · • · · • · WO 01/85772 275 280 285 PCT/GB01/02003 Leu His Asn 290 Leu Gly Ala Asp Gly Ile Gly Leu Phe 295 300 Arg Ser Glu Phe Leu Tyr Leu 305 Asn Arg Asp Thr Met Pro Ser Glu Asp 310 315 Glu Gin Tyr Glu 320 Val Tyr Ser Ala Ile Val Lys Lys Met Lys Gly Lys 325 330 Ser Val Thr 335 Ile Arg Thr Val Asp Leu Gly Val Asp Lys Asn Pro Arg 340 345 Trp Phe 350 Gly Lys Asn Ser Thr 355 Pro Asn Gly Ser Leu Asn Pro Ala Leu 360 Gly 365 Met Thr Gly Ile Arg Leu 370 cys Leu Ala Glu Pro Val Met Phe Arg 375 380 Thr Gin Met Arg Ala Ile Leu 385 Arg Ala Ala Ala His Gly Pro Val Arg 390 395 Met Met Trp Pro 400 Met Ile Thr Ser Val Ser Glu Val Arg Gin Cys Leu 405 410 Ile His Leu 415 Asp Thr Ala Gin Arg Gin Leu Ala Glu Arg Gly Asp Ala 420 425 Phe Gly 430 Lys Val Gly Ile Gly 435 Cys Met Ile Glu Ile Pro Ser Ala Ala 440 Leu 445 Thr Val Gly Ser Ile Leu 450 Lys Leu Val Asp Phe Ile Ser Val Gly 455 460 Thr Asn Asp Leu Ile Gin Tyr 465 Ile Leu Ser Val Asp Arg Gly Asp Asp 470 . 475 Ser Val Ser His 480 Leu Tyr Gin Pro Gly His Pro Ala Val Leu Lys Met 485 . 490 Leu Gin His 495 Val Ile Arg Thr Ala Asn Arg Met Asp Lys Asp Val Ser 500 505 Val Cys 510 Gly Glu Met Ala Gly 515 Asp Thr Ala Phe Thr Arg Val Leu Leu 520 Gly 525 Met Gly Leu Arg Arg Phe Ser Met Asn Pro Asn Asn Ile Leu Pro 155 Val Lys Asn Ile

WO 01/85772

530

Ile Leu. His 545

Ile Arg cys

Asn Ser Val

PCT/GB01/02003

535 540 Ser Asn Val 550 Gly Gin Leu Glu Ser 555 Asp Ile Val Lys Val 560 Glu Asp 565 Glu Glu Lys Ser Glu 570 Lys Leu Ile Lys Gin 575 Met Ser 580 Val Glu Glu Glu Ala 585 Asp Phe Lys Gly Arg 590 Lys

<210> 79 <211> 1062 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1062) <400> 79

atg gaa Met Glu 1 aaa Lys gaa ttc agg ata tta aat atc gta tcg gcc aag att tgg 48 Glu Phe Arg Ile 5 Leu Asn Ile 10 Val Ser Ala Lys Ile Trp 15 ggt gga ggc gaa caa tat gtc tat gat gtt tea aaa gca ttg ggg ctt 96 Gly Gly Gly Glu Gin Tyr Val Tyr Asp Val Ser Lys Ala Leu Gly Leu 20 25 30 '· cgg gac tgc aca atg ttt acc gcc gtc aat aaa aat aat gaa ttg atg 144 Arg Asp Cys Thr Met Phe Thr Ala Val Asn Lys Asn Asn Glu Leu Met 35 40 45 cac agg ega ttt tcc gaa gtt tet tcc gtt ttc acg aca cgc ctt cac 192 His Arg Arg Phe Ser Glu Val Ser Ser Val Phe Thr Thr Arg Leu His 50 55 60 acg ctc aac ggg ctg ttt tcg ctc tac gca ctt acc cgc ttt atc cgg 240 Thr Leu Asn Gly Leu Phe Ser Leu Tyr Ala Leu Thr Arg Phe Ile Arg 65 70 75 80 aaa aac cgc att tcc cac ctg atg ata cac acc ggc aaa att gcc gcc 288 Lys Asn Arg Ile Ser His Leu Met Ile His Thr Gly Lys Ile Ala Ala 85 90 95 tta tcc ata ctt ttg aaa aaá ctg acc ggg gtg cgc ctg ata ttt gtc 336

156

WO 01/85772

PCT/GB01/02003

Leu Ser Ile Leu Leu Lys Lys Leu Thr Gly Val Arg. Leu Ile Phe Val 100 105 110 aaa cat aat gtt gtc gcc aac aaa act gat ttt tat cac cgc ctg ata 384 Lys His Asn Val Val Ala Asn Lys Thr Asp Phe Tyr His Arg Leu Ile ¹¹⁵ 120 125 cag aaa aac acc gac cgc ttt att tgc gtt tcc cgt ctg gtt tac gat 432 Gin Lys Asn Thr Asp Arg Phe Ile Cys Val Ser Arg Leu Val Tyr Asp 130 135 140 gtg caa acc gcc gac aat ccc ttt aaa gaa aaa tac cgg att gtt cat 480 Val Gin Thr Ala Asp Asn Pro Phe Lys Glu Lys Tyr Arg Ile Val His 145 150 155 160 aac ggt atc gat acc ggc cgt ttc cct ccc cct caa gaa aaa ccc gac 528 Asn Gly Ile Asp Thr Gly Arg Phe Pro Pro Pro Gin Glu Lys Pro Asp 165 - — - 170 175 agc cgt ttt ttt acc gtc gcc tac gcc ggc agg atc agt ccg gaa aaa 576 Ser Arg Phe Phe Thr Val Ala Tyr Ala Gly Arg Ile Ser Pro Glu Lys 180 185 190 gga ttg gaa aac ctg att gaa gcc tgt gtg ata ctg cat cgg aaa tat 624 Gly Leu Glu Asn Leu Ile Glu Ala Cys Val Ile Leu His Arg Lys Tyr 195 200 205 cct caa atc agg ctc aaa ttg gca ggg gac gga cat ccg gat tat atg 672 Pro Gin Ile Arg Leu Lys Leu Ala Gly Asp Gly His Pro Asp Tyr Met 210 215 220 tgt cgc ctg aag cgg gac gta tet get tcg gga gca gaa cca ttt gtt 720 Cys Arg Leu Lys Arg Asp Val Ser Ala Ser Gly Ala Glu Pro Phe Val 225 230 235 240 tet ttt gaa ggg ttt acc gaa aaa ctt get tcg ttt tac cgc caa agc 768 Ser Phe Glu Gly Phe Thr Glu Lys Leu Ala Ser Phe Tyr Arg Gin Ser 245 250 255 gat gtc gtg gtt ttg ccc .agc ctc gtc ccg gag gca ttc ggt ttg tea 816 Asp Val Val Val Leu Pro Ser Leu val Pro Glu Ala Phe Gly Leu Ser 260 265 270 tta tgc gag gcg atg tac tgc ega acg gcg gtg att tcc aat act ttg 864 Leu Cys Glu Ala Met Tyr Cys Arg Thr Ala Val Ile Ser Asn Thr Leu 275 280 285 ggc gcg caa aag gaa att atc gaa cat cat caa tcg ggg att ctg ctg 912

157 • · α · · ·

WO 01/85772

PCT/GB01/02003

Gly Ala Gin Lys Glu Ile Ile Glu His His Gin Ser Gly Ile Leu Leu 290 295 300 gat agg ctg aca cct gaa tet ttg gcg gac gaa atc gaa cgc ctc gtc Asp Arg Leu Thr Pro Glu Ser Leu Ala Asp Glu Ile Glu Arg Leu Val 305 310 315 320 tta aac cct gaa acg aaa aac gca ctg gca acg gca gga cat caa tgc Leu Asn Pro Glu Thr Lys Asn Ala Leu Ala Thr Ala Gly His Gin Cys 325 330 335 gtc gcc aac cgt ttt acc atc aac cat acc gcc gac aaa tta ttg gat Val Ala Asn Arg Phe Thr Ile Asn His Thr Ala Asp Lys Leu Leu Asp 340 345 350 gca ata Ala Ile

960

1008

1056

1062 <210> 80 <211> 354 <212> PRT <213> Nelsseria meningitidis <400> 80

Met Glu 1 Lys Glu Phe 5 Arg Ile Leu Asn Ile 10 Val Ser Ala Lys Ile 15 Trp Gly Gly Gly Glu 20 Gin Tyr Val Tyr Asp 25 Val Ser Lys Ala Leu 30 Gly Leu Arg Asp cys 35 Thr Met Phe Thr Ala 40 Val Asn Lys Asn Asn 45 Glu Leu Met His Arg 50 Arg Phe Ser Glu Val 55 Ser Ser Val Phe Thr 60 Thr Arg Leu His Thr Leu 65 Asn Gly Leu Phe 70 Ser Leu Tyr Ala Leu 75 Thr Arg Phe Ile Arg 80 Lys Asn Arg Ile Ser 85 His Leu Met Ile His 90 Thr Gly Lys Ile Ala 95 Ala Leu Ser Ile Leu 100 Leu Lys Lys Leu Thr 105 Gly Val Arg Leu Ile 110 Phe Val Lys His Asn Val Val Ala Asn Lys Thr Asp Phe Tyr His Arg Leu Ile

115 120 125

158

WO 01/85772

PCT/GB01/02003

Gin Lys Asn Thr Asp Arg Phe Ile Cys 130 135

Val Gin Thr Ala Asp Asn Pro Phe Lys 145 150

Asn Gly Ile Asp Thr Gly Arg Phe Pro 165

Ser Arg Phe Phe Thr Val Ala Tyr Ala 180 185

Gly Leu Glu Asn Leu Ile Glu Ala Cys 195 200

Pro Gin Ile Arg Leu Lys Leu Ala Gly 210 215

Cys Arg Leu Lys Arg Asp Val Ser Ala 225 230

Ser Phe Glu Gly Phe Thr Glu Lys Leu 245

Asp Val Val Val Leu Pro Ser Leu Val 260 265

Leu Cys Glu Ala Met Tyr Cys Arg Thr 275 280

Gly Ala Gin Lys Glu Ile Ile Glu His 290 295

Asp Arg Leu Thr Pro Glu Ser Leu Ala 305 310

Leu Asn Pro Glu Thr Lys Asn Ala Leu 325

Val Ala Asn Arg Phe Thr Ile Asn His 340 345

Ala Ile

Val Ser Arg Leu Val Tyr Asp 140

Glu Lys Tyr Arg Ile Val His 155 160

Pro Pro Gin Glu Lys Pro Asp 170 175

Gly Arg Ile Ser Pro Glu Lys 190 val Ile Leu His Arg Lys Tyr 205

Asp Gly His Pro Asp Tyr Met 220

Ser Gly Ala Glu Pro Phe Val 235 240

Ala Ser Phe Tyr Arg Gin Ser 250 255

Pro Glu Ala Phe Gly Leu Ser 270

Ala Val Ile Ser Asn Thr Leu 285

His Gin Ser Gly Ile Leu Leu 300

Asp Glu Ile Glu Arg Leu Val 315 320

Ala Thr Ala Gly His Gin Cys 330 335

Thr Ala Asp Lys Leu Leu Asp 350

159 ···· ····

WO 01/85772 PCT/GB01/02003 <210> 81 <211> 795 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(795) <400> 81

atg Met 1 gcg gct gtt ttt tet gta tgt gcc tgt ttt atg tgt tcc tgc ctt 48 Ala Ala Val Phe Ser Val 5 Cys Ala Cys 10 Phe Met Cys Ser Cys 15 Leu gtt gtc aaa aat acc gtt atc gga age gga cgc acc aaa atc gcc gtg 96 Val Val Lys Asn Thr Val Ile Gly Ser Gly Arg Thr Lys Ile Ala Val 20 25 30 ccg ctt gtc gcc cgc gat gcc gcc gta ctt tcc gcc gta ctt gac caa 144 Pro Leu Val Ala Arg Asp Ala Ala Val Leu Ser Ala Val Leu Asp Gin 35 40 45 atc aaa aac ctg ccc ttc gat atc gtc gag ttc cgc gcc gat ttt ttg 192 Ile Lys Asn Leu Pro Phe Asp Ile Val Glu Phe Arg Ala Asp Phe Leu 50 55 60 gaa tgc gcg ggc age atc ggc gaa gta ttg cgc cac acg cag acc gtc 240 Glu Cys Ala Gly Ser Ile Gly Glu Val Leu Arg His Thr Gin Thr Val 65 70 75 80 cgc gac gcg cta ccc gac aag ccg ctg ctg ttt acg ttc aga cgg cat 288 Arg Asp Ala Leu Pro Asp Lys Pro Leu Leu Phe Thr Phe Arg Arg His 85 90 95 ggc gaa ggc ggc tcg ttc ccg tgt tcg gac gat tat tat ttt gaa ctg 336 Gly Glu Gly Gly Ser Phe Pro Cys Ser Asp Asp Tyr Tyr Phe Glu Leu 100 105 110 ctc gac gcg ctg atc gaa age cgc ctg ccc gac atc atc gac atc gag 384 Leu Asp Ala Leu Ile Glu 'Ser Arg Leu Pro Asp Ile Ile Asp Ile Glu 115 120 125 ctg ttt tcc ggc gaa acc gcc gtc cgg tgc gcc gtg gca aat gct caa 432 Leu Phe Ser Gly Glu Thr Ala Val Arg Cys Ala Val Ala Asn Ala Gin 130 135 140 aaa aac ggc atc gcc gcc ctg ctc tgc aat cat gag ttt cac cgc acg 480 Lys Asn Gly Ile Ala Ala Leu Leu Cys Asn His Glu Phe His Arg Thr

160 ·· ·« ·· • · · · · ·

WO 01/85772

PCT/GB01/02003

145 150 155 160 ccg ccg caa gaa gaa atc gta tgc cgt ctg aaa cag atg gag gac tgc 528 Pro Pro Gin Glu Glu Ile Val Cys Arg Leu Lys Gin Met Glu Asp Cys 165 170 175 ggc gcg gac atc tgc aaa att gcg gtg atg ccg caa agc gcg gaa gat 576 Gly Ala Asp Ile Cys Lys Ile Ala Val Met Pro Gin Ser Ala Glu Asp 180 185 190 gtg ctg act ttg ctt tcc gcc acg ctc aaa gcg aaa gag ctt gcc gcc 624 Val Leu Thr Leu Leu Ser Ala Thr Leu Lys Ala Lys Glu Leu Ala Ala 195 200 205 aaa ccg att gtt acg atg tcg atg ggg cag acg ggg gcg gtc agc cgg 672 Lys Pro Xle Val Thr Met Ser Met Gly Gin Thr Gly Ala Val Ser Arg 210 215 220 ctt gcc gga cag gtg ttc ggc tea agc atc acg ttc ggt tcg gga acg 720 Leu Ala Gly Gin Val Phe Gly Ser Ser Ile Thr Phe Gly Ser Gly Thr 225 230 235 240 caa aac tcc gcg ccg ggg caa atc ggc gta tcc gcc ctc cgt gcg aca 768 Gin Asn Ser Ala Pro Gly Gin Ile Gly Val Ser Ala Leu Arg Ala Thr 245 250 255 ctc gac tgc ctc gaa aac gga gca gac 795 Leu Asp Cys Leu Glu Asn Gly Ala Asp

260 265 <210> 82 <211> 265 <212> PRT

<213> Neisseria meningitidis <400> 82 Met Ala Ala Val Phe Ser Val Cys Ala Cys Phe Met Cys Ser Cys Leu 15 10 15 Val Val Lys Asn Thr Val Ile Gly Ser Gly Arg Thr Lys Ile Ala Val 20 25 30 Pro Leu Val Ala Arg Asp Ala Ala Val Leu Ser Ala Val Leu Asp Gin 35 40 45 Ile Lys Asn Leu Pro Phe Asp Ile Val Glu Phe Arg Ala Asp Phe Leu

50 55 60

161

WO 01/85772 PCT/GBO1/02003 Glu Cys Ala Gly Ser Ile Gly Glu Val Leu Arg His Thr Gin Thr Val 65 70 75 80 Arg Asp Ala Leu Pro Asp Lys Pro Leu Leu Phe Thr Phe Arg Arg His 85 90 95 Gly Glu Gly Gly Ser Phe Pro Cys Ser Asp Asp Tyr Tyr Phe Glu Leu 100 105 110 Leu Asp Ala Leu ile Glu Ser Arg Leu Pro Asp Ile Ile Asp Ile Glu 115 120 125 Leu Phe Ser Gly Glu Thr Ala Val Arg Cys Ala Val Ala Asn Ala Gin 130 135 140 Lys Asn Gly Ile Ala Ala Leu Leu Cys Asn His Glu Phe His Arg Thr 145 150 155 160 Pro Pro Gin Glu Glu Ile Val Cys Arg Leu Lys Gin Met Glu Asp Cys 165 170 175 Gly Ala Asp Ile Cys Lys Ile Ala Val Met Pro Gin Ser Ala Glu Asp 180 185 190 Val Leu Thr Leu Leu Ser Ala Thr Leu Lys Ala Lys Glu Leu Ala Ala 195 200 205 Lys Pro Ile Val Thr Met Ser Met Gly Gin Thr Gly Ala Vál Ser Arg 210 215 220 Leu Ala Gly Gin Val Phe Gly Ser Ser Ile Thr Phe Gly Ser Gly Thr 225 230 235 240 Gin Asn Ser Ala Pro Gly Gin Ile Gly Val Ser Ala Leu Arg Ala Thr 245 250 255 Leu Asp Cys Leu Glu Asn Gly Ala Asp

260 265 <210> 83 <211> 876 <212> DNA <213> Neisseria meningitidis

<220>

162 • · 9 99 9

99 *9 9« • · f · · v · · * · · • »49» · « « • ···· 9*9» « • · · 9 9 · 9 9 ·

9999 9499 99 ··· 99 «·

WO 01/85772 PCT/GB01/02003 <221> CDS <222> (1)..(876) <400> 83

ttg Leu 1 acc gtc ega acg aaa aag acg gcg cat tat acc cta ttc cat tcc 48 Thr Val Arg Thr Lys Lys 5 Thr Ala His 10 Tyr Thr Leu Phe His 15 Ser gac ega aaa ccg aac atg act act ctc aaa ccc gcc ctg ccc get tat 96 Asp Arg Lys Pro Asn Met Thr Thr Leu Lys Pro Ala Leu Pro Ala Tyr 20 25 30 ctg gac aac atc cgc atc atc ctc acg cgc acc age cat ccc gcc aac 144 Leu Asp Asn Ile Arg Ile Ile Leu Thr Arg Thr Ser His Pro Ala Asn 35 40 45 atc ggc tet gcc gcg cgc gcg atg aaa aca atg ggt ctg cac aaa ctg 192 Ile Gly Ser Ala Ala Arg Ala Met Lys Thr Met Gly Leu His Lys Leu 50 55 60 acc atc gtc gcc cca aat ctg atg gca acg ccg atg acg gaa aac ccg 240 Thr Ile Val Ala Pro Asn Leu Met Ala Thr Pro Met Thr Glu Asn Pro 65 70 75 80 ccc gtg ttt gac ccg gag cat cct caa tcg ttt aaa tta ccg gaa gaa 288 Pro Val Phe Asp Pro Glu His Pro Gin Ser Phe Lys Leu Pro Glu Glu 85 90 95 age ttc atc ctc get tcc ggc gcg gca gac gtt ttg gaa aat gcc acc 336 Ser Phe Ile Leu Ala Ser Gly Ala Ala Asp Val Leu Glu Asn Ala Thr 100 105 110 att gcc get tet ttg gac gaa gcc ctt gcc gac acc acc atc gcc tgc 384 Ile Ala Ala Ser Leu Asp Glu Ala Leu Ala Asp Thr Thr Ile Ala Cys 115 120 125 gcc ctg acc age cgc cgc cgc gaa att act gcg ccg ctg caa acc ccg 432 Ala Leu Thr Ser Arg Arg Arg Glu Ile Thr Ala Pro Leu Gin Thr Pro 130 135 140 cgc gat ttg gta tcc gaa' tta ctg cag acc gca aac ega ggc gag aaa 480 Arg Asp Leu Val Ser Glu Leu Leu Gin Thr Ala Asn Arg Gly Glu Lys 145 150 155 160 gtg gca ctg gtt ttc ggc aac gag act ttc ggc ttg age atc gaa gaa 528 Val Ala Leu Val Phe Gly Asn Glu Thr Phe Gly Leu Ser Ile Glu Glu 165 170 175

163 φφ φφ φφ '* φ φ · « « • · φ · φφφφ φ φ > φ φ φφφφ φφφφ φφ φφφφ φ φφ φφ « φ φ * φ φ » φφφφ φ • φ φ φ φ φφφ φφ φφ

W0 01/85772

gtc caa gcc tgc aac ega ctg atg acc Val Gin Ala Cys 180 Asn Arg Leu Met Thr 185 ttc tcg ctc aac ctc gcc caa gcc gtg Phe Ser Leu 195 Asn Leu Ala Gin Ala 200 Val ttc age caa acc ggt tcg ccc atg acc Phe Ser 210 Gin Thr Gly Ser Pro 215 Met Thr get gcg acc cac gag caa atc aaa ggc Ala 225 Ala Thr His Glu Gin 230 Ile Lys Gly gtg atg aac gac atc ggc ttt ttc aac Val Met Asn Asp Ile 245 Gly Phe Phe Asn atg cgc cgt atg cag age ctg ttc gga Met Arg Arg Met 260 Gin Ser Leu Phe Gly 265 gac atc gat atc ctg cgc ggt ttt ttc Asp Xle Asp 275 Xle Leu Arg Gly Phe 280 Phe

PCT/GBO1/02003 atc aac ggc aat ccc gac tat 576 Ile Asn Gly Asn Pro 190 Asp Tyr cag gtc gtg tgc tac gaa atc 624 Gin Val Val Cys 205 Tyr Glu Ile cat ctt caa caa gaa gac cac 672 His Leu Gin 220 Gin Glu Asp His atg gtc gcc cac atg gaa age 720 Met Val 235 Ala His Met Glu Ser 240 cgc cgc aac ggc gag cgt ctg 768 Arg Arg 250 Asn Gly Glu Arg 255 Leu cgc gcc aac acg caa acc gaa 816 Arg Ala Asn Thr Gin 270 Thr Glu aat acc gtc age cat cgt atc 864 Asn Thr Val Ser His Arg Ile

285

cat aaa aaa gac 876 His Lys 290 Lys Asp

<210> 84 <211> 292 <212> PRT <213> Neisseria meningitidis <400> 84 Leu Thr Val Arg Thr Lys,'Lys Thr Ala His Tyr Thr Leu Phe His Ser 1 5 10 15 Asp Arg Lys Pro Asn Met Thr Thr Leu Lys Pro Ala Leu Pro Ala Tyr 20 25 30 Leu Asp Asn Ile Arg Ile Ile Leu Thr Arg Thr Ser His Pro Ala Asn 35 40 45

164

• ’· • · • fc • • · » · • • » · · · • • • • • • · • fcfc • · • · • · · · • · • · • ♦ • « • « WO 01/85772 PCT/GBO1/02003 Ile Gly Ser Ala Ala Arg Ala Met Lys Thr Met Gly Leu His Lys Leu 50 55 60 Thr Ile Val Ala Pro Asn Leu Met Ala Thr Pro Met Thr Glu Asn Pro 65 70 75 80 Pro Val Phe Asp Pro Glu His Pro Gin Ser Phe Lys Leu Pro Glu Glu 85 90 95 Ser Phe Ile Leu Ala Ser Gly Ala Ala Asp Val Leu Glu Asn Ala Thr 100 105 110 Ile Ala Ala Ser Leu Asp Glu Ala Leu Ala Asp Thr Thr Ile Ala Cys 115 120 125 Ala Leu Thr Ser Arg Arg Arg Glu Ile Thr Ala Pro Leu Gin Thr Pro 130 135 140 Arg Asp Leu Val Ser Glu Leu Leu Gin Thr Ala Asn Arg Gly Glu Lys 145 150 155 160 Val Ala Leu Val Phe Gly Asn Glu Thr Phe Gly Leu Ser Ile Glu Glu 165 170 175 Val Gin Ala Cys Asn Arg Leu Met Thr Ile Asn Gly Asn Pro Asp Tyr 180 185 190 Phe Ser Leu Asn Leu Ala Gin Ala Val Gin Val Val Cys Tyr Glu Ile 195 200 205 Phe Ser Gin Thr Gly Ser Pro Met Thr His Leu Gin Gin Glu Asp His 210 215 220 Ala Ala Thr His Glu Gin Ile Lys Gly Met Val Ala His Met Glu Ser 225 230 235 240 Val Met Asn Asp Ile Gly Phe Phe Asn Arg Arg Asn Gly Glu Arg Leu 245 250 255 Met Arg Arg Met Gin Ser Leu Phe Gly Arg Ala Asn Thr Gin Thr Glu 260 265 270 Asp Ile Asp Ile Leu Arg Gly Phe Phe Asn Thr Val Ser His Arg Ile 275 280 285 His Lys Lys Asp

290

155

WO 01/85772

PCT/GBO1/02003 <2l0> 85 <211> 1545 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1545) <400> 85

gtg cgt ctc aat cat ttc ata atg ata gcg att att ata tat gtg att Ile 48 Val 1 Arg Leu Asn His 5 Phe Ile Met Ile Ala 10 Ile Ile Ile Tyr Val 15 tcc cct gca aac aag ccg gcc cgc cgc cac age gtt ccc act tat ccg 96 Ser Pro Ala Asn- Lys Pro Ala Arg Arg His Ser Val Pro Thr Tyr Pro 20 25 30 gct ttg cct tat aat tgc ttt ttt tat gta aca gat tta cct atg aat 144 Ala Leu Pro Tyr Asn Cys Phe Phe Tyr Val Thr Asp Leu Pro Met Asn 35 40 45 ttc ccc aaa aca gcg gcc tcc ctg ctg ctg ctt ctc gcc tcc ctc gcc 192 Phe Pro Lys Thr Ala Ala Ser Leu Leu Leu Leu Leu Ala Ser Leu Ala 50 55 60 gca cac gcg ctc gat aca ggt cgc att ccg caa aac gaa atc gcc gta 240 Ala His Ala Leu Asp Thr Gly Arg Ile Pro Gin Asn Glu Ile Ala Val 65 70 75 80 tat gtc caa gag ctt gac age gga aaa gtc atc att gac cac cgc tcg 288 Tyr Val Gin Glu Leu Asp Ser Gly Lys Val Ile Ile Asp His Arg Ser 85 90 95 gat gtc ccc gtc aac ccc gcc tcc aca atg aaa ctc gtt acc gcg ttt 336 Asp Val Pro Val Asn Pro Ala Ser Thr Met· Lys Leu Val Thr Ala Phe 100 105 110 gcc gcc ttc aaa acc ttc ggc age aat tac cgc tgg gcg acc gag ttt 384 Ala Ala Phe Lys Thr Phe Gly Ser Asn Tyr Arg Trp Ala Thr Glu Phe 115 120 125 aaa age aac ggt acg gta aac gac ggc acg ctt gac gga aac ctg tat 432 Lys Ser Asn Gly Thr Val Asn Asp Gly Thr Leu Asp Gly Asn Leu Tyr 130 135 140

166

β » · e e β • • · 9 9 9 • · β 9 9 • 9 9 9 9 9 9 9 • · • · • · • · WO 01/85772 PCT/GBO1/02003 tgg gcg ggc age ggc gac ccc gtt ttc aat cag gaa aac ctg ctt gcc 480 Trp Ala Gly Ser Gly Asp Pro Val Phe Asn Gin Glu Asn Leu Leu Ala 145 150 155 160 gtc caa cgc cag ttg cgc gaa caa ggc ata cgc aat atc acg gga cac 528 Val Gin Arg Gin Leu Arg Glu Gin Gly Ile Arg Asn Ile Thr Gly His 165 170 175 ctg atg ctc gac cac age ctg tgg ggc gaa gtc ggc age CCC gac gat 576 Leu Meť Leu Asp His Ser Leu Trp Gly Glu Val Gly Ser Pro Asp Asp 180 185 190 ttc gaa gcc gac age ggt tcg ccg ttt atg acg ccc CCC aat cca act 624 Phe Glu Ala Asp Ser Gly Ser Pro Phe Met Thr Pro Pro Asn Pro Thr 195 200 205 atg ctg tet gcc ggt atg gtt atg gtg cgc gcc gaa cgc aat gcc gcc 672 Met Leu Ser Ala Gly Met Val Met Val Arg Ala Glu Arg Asn Ala Ala 210 215 220 gac agt acc gac atc ctc acc gat ccg cct ttg ccg cat att ttc gcc 720 Asp Ser Thr Asp Ile Leu Thr Asp Pro Pro Leu Pro His Ile Phe Ala 225 230 235 240 caa aac aac ttg aaa att acc gcc tcc caa get gcc tgc cct tcg atc 768 Gin Asn Asn Leu Lys Ile Thr Ala Ser Gin Ala Ala Cys Pro Ser Ile 245 250 255 aaa aaa ctg atg cgt gca tet ttt tcg gac aat acg ctg aaa ttg cgc 816 Lys Lys Leu Met Arg Ala Ser Phe Ser Asp Asn Thr Leu Lys Leu Arg 260 265 270 ggc aat att ccc gag age tgt ttg ggc aag cct gtc ggt gtc cgg atg 864 Gly Asn Ile Pro Glu Ser Cys Leu Gly Lys Pro Val Gly Val Arg Met 275 280 285 ttc gcg ctt gac gaa ctg atc cgg caa agt ttt acc aac cac tgg ctg 912 Phe Ala Leu Asp Glu Leu Ile Arg Gin Ser Phe Thr Asn His Trp Leu 290 295 300 ctc ggc ggc gga cgg att' tea gac ggc atc ggc ata tcc gac acg ccg 960 Leu Gly Gly Gly Arg Ile Ser Asp Gly Ile Gly Ile Ser Asp Thr Pro 305 310 315 320 gaa ggc gcg cag acg ctt gcc gtt gca cac tea aag ccg atg aag gaa 1008 Glu Gly Ala Gin Thr Leu Ala Val Ala His Ser Lys Pro Met Lys Glu 325 330 335

167

WO 01/85772

PCT/GB01/02003

att ttg acg gac atg aac aag cgt Lys Arg tcg gac aat cta att gcg cgt tcc 1056 Ile Leu Thr Asp Met Asn 340 Ser 345 Asp Asn Leu Ile Ala Arg 350 Ser gtc ttc ctc aaa ctc ggc ggc gac ggc aaa ctg ccc gcc gtt tcc gaa 1104 val Phe Leu Lyš Leu Gly Gly Asp Gly Lys Leu Pro Ala Val Ser Glu 355 360 365 cag gca gcg tet gcc gtc cgg cgt gaa ctt gcc gtg tcg ggc atc gat 1152 Gin Ala Ala Ser Ala Val Arg Arg Glu Leu Ala Val Ser Gly Ile Asp 370 375 380 gtt gcg gat ttg gtt ttg gaa aac ggt tca ggt ctg tcc aga aaa gaa 1200 Val Ala Asp Leu Val Leu Glu Asn Gly Ser Gly Leu Ser Arg Lys Glu 385 390 395 400 agg gta acg gcg aga atg atg gcg caa atg ttg gaa acg get tat ttc 1248 Arg Val Thr Ala Arg Met Met Ala Gin Met Leu Glu Thr Ala Tyr Phe 405 410 415 age ccg ttt gca caa gat ttc atc gat acg ctg ccc atc gcc ggc aca 1296 Ser Pro Phe Ala Gin Asp Phe Ile Asp Thr Leu Pro Ile Ala Gly Thr 420 425 430 gac ggg act tta cgc aac cgc ttc aaa caa age ggc ggg ctg ttg cgc 1344 Asp Gly Thr Leu Arg Asn Arg Phe Lys Gin Ser Gly Gly Leu Leu Arg 435 440 445 tta aaa acc ggc acg ctc aac aat gtc cgc gcc ctt gca ggt tat tgg 1392 Leu Lys Thr Gly Thr Leu Asn Asn Val Arg Ala Leu Ala Gly Tyr Trp 450 455 460 ctg ggc gac aaa ccg atg gcg gtg gtc gtc atc atc aac age ggc cgc 1440 Leu Gly Asp Lys Pro Met Ala Val Val Val Ile Ile Asn Ser Gly Arg 465 470 475 480 gcc gtt tcc ctg ctg ccc gac ttg gac aac ttc gtt gcc aac aac atc 1488 Ala Val Ser Leu Leu Pro Asp Leu Asp Asn Phe Val Ala Asn Asn Ile 485 490 495 atc tcc ggc ggc gat ggc tgg ctg gat gcg aaa ctg atg tgc aaa gaa 1536 Ile Ser Gly Gly Asp Gly Trp Leu Asp Ala Lys Leu Met Cys Lys Glu 500 505 510

cgc ega gcc 1545

Arg Arg Ala

515

168

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PCT/GBO1/02003 <210> 86 <211> 515 <212> PRT <213> Neisseria meningitidis <400> 86

Val 1 Arg Leu Asn His 5 Phe Ile Met Ile Ala 10 Ile Ile Ile Tyr Val 15 Ile Ser Pro Ala Asn 20 Lys Pro Ala Arg Arg 25 His Ser Val Pro Thr 30 Tyr Pro Ala Leu Pro 35 Tyr Asn Cys Phe Phe 40 Tyr Val Thr Asp Leu 45 Pro Met Asn Phe Pro 50 Lys Thr Ala Ala Ser 55 Leu Leu Leu Leu Leu 60 Ala Ser Leu Ala Ala 65 His Ala Leu Asp Thr 70 Gly Arg Ile Pro Gin 75 Asn Glu Ile Ala Val 80 Tyr Val Gin Glu Leu 85 Asp Ser Gly Lys Val 90 Ile Ile Asp His Arg 95 Ser Asp Val Pro Val 100 Asn Pro Ala Ser Thr 105 Met Lys Leu Val Thr 110 Ala Phe Ala Ala Phe 115 Lys Thr Phe Gly Ser 120 Asn Tyr Arg Trp Ala 125 Thr Glu Phe Lys Ser 130 Asn Gly Thr Val Asn 135 Asp Gly Thr Leu Asp 140 Gly Asn Leu Tyr Trp 145 Ala Gly Ser Gly Asp 150 Pro Val Phe Asn Gin 155 Glu Asn Leu Leu Ala 160 Val Gin Arg Gin Leu 165 Arg Glu Gin Gly Ile 170 Arg Asn Ile Thr Gly 175 His Leu Met Leu Asp 180 His Ser Leu Trp Gly 185 Glu Val Gly Ser Pro 190 Asp Asp Phe Glu Ala 195 Asp Ser Gly Ser Pro 200 Phe Met Thr Pro Pro 205 Asn Pro Thr Met Leu Ser Ala Gly Met Val Met Val Arg Ala Glu Arg Asn Ala Ala

210 215 220

169

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Asp 225 Ser Thr Asp Ile Leu 230 Thr Asp Pro Pro Leu 235 Pro His Ile Phe Ala 240 Gin Asn Asn Leu Lys 245 Ile Thr Ala Ser Gin 250 Ala Ala Cys Pro Ser 255 Zle Lys Lys Leu Met 260 Arg Ala Ser Phe Ser 265 Asp Asn Thr Leu Lys 270 Leu Arg Gly Asn Ile 275 Pro Glu Ser Cys Leu 280 Gly Lys Pro Val Gly 285 Val Arg Met Phe Ala 290 Leu Asp Glu Leu Ile 295 Arg Gin Ser Phe Thr 300 Asn His Trp Leu Leu 305 Gly Gly Gly Arg Ile 310 Ser Asp Gly Ile Gly 315 Ile Ser Asp Thr Pro 320 Glu Gly Ala Gin Thr 325 Leu Ala Val Ala His 330 Ser Lys Pro Met Lys 335 Glu Ile Leu Thr Asp 340 Met Asn Lys Arg Ser 3-45 Asp Asn Leu Ile Ala 350 Arg Ser Val Phe Leu 355 Lys Leu Gly Gly Asp 360 Gly Lys Leu Pro Ala 365 Val Ser Glu Gin Ala 370 Ala Ser Ala Val Arg 375 Arg Glu Leu Ala Val 380 Ser Gly Ile Asp Val 385 Ala Asp Leu Val Leu 390 Glu Asn Gly Ser Gly 395 Leu Ser Arg Lys Glu 400 Arg Val Thr Ala Arg 405 Met Met Ala Gin Met 410 Leu Glu Thr Ala Tyr 415 Phe Ser Pro Phe Ala 420 Gin Asp Phe Ile Asp 425 Thr Leu Pro Ile Ala 430 Gly Thr Asp Gly Thr 435 Leu Arg Asn Arg Phe 440 Lys Gin Ser Gly Gly 445 Leu Leu Arg Leu Lys 450 Thr Gly Thr Leu Asn 455 Asn Val Arg Ala Leu 460 Ala Gly Tyr Trp Leu Gly Asp Lys Pro Met Ala Val Val Val Ile Ile Asn Ser Gly Arg

465

470

475

480

170

WO 01/85772 PCT/GB01/02003

Ala Val Ser Leu Leu Pro Asp Leu Asp Asn Phe Val Ala Asn Asn Ile 485 490 495

Ile Ser Gly Gly Asp Gly Trp Leu Asp Ala Lys Leu Met Cys Lys Glu 500 505 510

Arg Arg Ala 515 <210> 87 <211> 840 <212> DNA <213> Neisseria meningitidis ;

<220>

<221> CDS <222> (1)..(840) <400> 87

atg gat Met Asp 1 aaa gaa ega att tta acc ccc gca gtc gtg ttt tcc gtc gca 48 Lys Glu Arg 5 Ile Leu Thr Pro Ala 10 Val Val Phe Ser Val 15 Ala ctg ctg cat ttg gca atg gtg gca ttg ctc tgg cag gcg cac aag ctg 96 Leu Leu His Leu Ala Met Val Ala Leu Leu Trp Gin Ala His Lys Leu 20 25 30 ccc gtg ata gag tca ggc aat gtt att gaa ttt gtc gat ttg ggc gat 144 Pro Val Ile Glu Ser Gly Asn Val Ile Glu Phe Val Asp Leu Gly Asp 35 40 45 ttt ggc gga ggg gac ggc gca ccc gaa ggt gca ggc gcg cct gcc gcg 192 Phe Gly Gly Gly Asp Gly Ala Pro Glu Gly Ala Gly Ala Pro Ala Ala 50 55 60 ccc gaa ccg caa ccc gtg ccc gag ccg ccc aaa cct gtc gag ccg ccc 240 Pro Glu Pro Gin Pro Val ;Pro Glu Pro Pro Lys Pro Val Glu Pro Pro 65 70 75 80 aag ccg gtt ttg aag ccg gtg gtt acg aaa aag gcg gat gcg gat att 288 Lys Pro Val Leu Lys Pro Val Val Thr Lys Lys Ala Asp Ala Asp Ile 85 90 95 cag cag cct aag gaa gag ccg aaa cct gaa gaa aag ccg aaa ccc gaa 336 Gin Gin Pro Lys Glu Glu Pro Lys Pro Glu Glu Lys Pro Lys Pro Glu

171

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100 105 110

gaa Glu aaa ccg aaa cca gag cct aag ccg gaa gcg aag cct gtc ccg aaa 384 Lys Pro 115 Lys Pro Glu pro Lys 120 Pro Glu Ala Lys Pro 125 Val Pro Lys ccg gcg gaa aaa ccg gtc gag aag ccg tet gaa aaa cct gcc gaa cat 432 Pro Ala Glu Lys Pro Val Glu Lys Pro Ser Glu Lys Pro Ala Glu His 130 135 140 ccc ggc aat gct tet gcc aaa gca gac agc gag cag ggc aat ggg gaa 480 Pro Gly Asn Ala Ser Ala Lys Ala Asp Ser GlU Gin Gly Asn Gly Glu 145 150 155 160 gat aag gga acc ggt atc aaa gga gac gga acg ggg cgc gga gaa ggc 528 Asp Lys Gly Thr Gly Ile Lys GÍy Asp Gly Thr Gly Arg Gly Glu Gly 165 170 175 agc ggt aaa ggt agc ggc ggt gtc aaa ggc gaa cac ggg gaa gga gcc 576 Ser Gly Lys Gly Ser Gly Gly Val Lys Gly Glu His Gly Glu Gly Ala 180 185 190 ggc agc agc aaa ggc aat cct tta cgc gcc aac ggc agc att ccg cgc 624 Gly Ser Ser Lys Gly Asn Pro Leu Arg Ala Asn Gly Ser Ile Pro Arg 195 200 205 ccg gct tat ccc acg ctt tet atg gag aat gat gag cag ggt acg gtt 672 Pro Ala Tyr Pro Thr Leu Ser Met Glu Asn Asp Glu Gin Gly Thr Val 210 215 220 gtt ttg agc gtt ttg gtt tet ccg ggc ggt cat gtt gag tcc gtt aaa 720 Val Leu Ser Val Leu Val Ser Pro Gly Gly His Val Glu Ser Val Lys 225 230 235 240 atc gtg aaa agc agt ggt ttt tcc cgg ttg gac aat gcg gca cgc aag 768 Ile Val Lys Ser Ser Gly Phe Ser Arg Leu Asp Asn Ala Ala Arg Lys 245 250 255 gcg gcg caa aac ggg cat ttt caa gcc aat gcc tgg acg gag ttt aaa 816 Ala Ala Gin Asn Gly His- Phe Gin Ala Asn Ala Trp Thr Glu Phe Lys 260 265 270 gtc ccc gtc aag ttt gaa ttg aat 840 Val Pro Val Lys Phe Glu Leu Asn 275 280

<210> 88

172 ·♦ ·· ·· · ···

WO 01/85772 PCT/GB01/02003 <211> 280 <212> PRT <213> Neisseria meningitidis <400> 88

Met Asp Lys Glu Arg Ile Leu Thr Pro Ala Val Val Phe Ser Val Ala 1 5 10 15 Leu Leu His Leu Ala Met Val Ala Leu Leu Trp Gin Ala His Lys Leu 20 25 30 Pro Val Ile Glu Ser Gly Asn Val Ile Glu Phe Val Asp Leu Gly Asp 35 40 45 Phe Gly Gly Gly Asp Gly Ala Pro Glu Gly Ala Gly Ala Pro Ala Ala 50 55 · 60 Pro Glu Pro Gin Pro Val Pro Glu Pro Pro Lys Pro Val Glu Pro Pro 65 70 75 80 Lys Pro Val Leu Lys Pro Val Val Thr Lys Lys Ala Asp Ala Asp Ile 85 90 95 Gin Gin Pro Lys Glu Glu Pro Lys Pro Glu Glu Lys Pro Lys Pro Glu 100 105 110 Glu Lys Pro Lys Pro Glu Pro Lys Pro Glu Ala Lys Pro Val Pro Lys 115 120 125 Pro Ala Glu Lys Pro Val Glu Lys Pro Ser Glu Lys Pro Ala Glu His 130 135 140 Pro Gly Asn Ala Ser Ala Lys Ala Asp Ser Glu Gin Gly Asn Gly Glu 145 150 155 160 Asp Lys Gly Thr Gly Ile Lys Gly Asp Gly Thr Gly Arg Gly Glu Gly 165 170 175 Ser Gly Lys Gly Ser Gly Gly Val Lys Gly Glu His Gly Glu Gly Ala 180 185 190 Gly Ser Ser Lys Gly Asn Pro Leu Arg Ala Asn Gly Ser Ile Pro Arg 195 200 205 Pro Ala Tyr Pro Thr Leu Ser Met Glu Asn Asp Glu Gin Gly Thr Val 210 215 220 Val Leu Ser Val Leu Val Ser Pro Gly Gly His Val Glu Ser Val Lys

173

00 00 0«

0 · e 0 β · « • 000

0 0 0 0 0

0 0 0 0 00000000 0 0 0

00 0 00 0

0 0 0

0 0«

0 0 0

0 0 0 0

00 00

WO 01/85772

PCT/GB01/02003

225 230 235 240 Ile Val Lys Ser Ser Gly Phe Ser Arg Leu Asp Asn Ala Ala Arg Lys 245 250 255 Ala Ala Gin Asn Gly His Phe Gin Ala Asn Ala Trp Thr Glu Phe Lys 260 265 270 Val Pro val Lys Phe Glu Leu Asn

275

280 <210> 89 <211> 1584 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1584, <400> 89 atg gca ctt ttc ctc age ata ttc ccc atc gtc ctg ctg att tgg ctg 48

Met Ala Leu Phe Leu Ser Ile Phe Pro Ile Val Leu Leu Ile Trp Leu

1 5 10 15 atg gtg aaa aaa aac agt atg ccc tcc tac gtc gcg ctg ccg att acc 96

Met Val Lys Lys Asn Ser Met Pro Ser Tyr Val Ala Leu Pro Ile Thr

20 25 30 gca gtg ctg att tac gcc atc aaa ctt ttc tac ttc gac gat gcg ggc 144

Ala Val Leu Ile Tyr Ala Ile Lys Leu Phe Tyr Phe Asp Asp Ala Gly

35 40 45 atg ctg ctc aac gcc acc gcc gct tcc ggc ctc gtc aaa acg ctc acg 192

Met Leu Leu Asn Ala Thr Ala Alá Ser Gly Leu Val Lys Thr Leu Thr

50 55 60 ccg att acc gtg att ttc'ggc gcg att atg ttc aac cgt atg atg gaa 240

Pro Ile Thr Val Ile Phe Gly Ala Ile Met Phe Asn Arg Met Met Glu

65 70 75 80 acc acg ggc tgc atc gat gtc atc cgc aaa tgg ctg gcg acc atc age 288

Thr Thr Gly Cys Ile Asp Val Ile Arg Lys Trp Leu Ala Thr Ile Ser

85 90 95 ccc aac ccc gta gcg caa ctg atg att atc ggc tgg gct ttt gcc ttt 336

174

WO 01/85772 PCT/GB01/02003

·· u* 99 4 44 9944 • · · · 9 9 ·· e · 4 • 9 9 9 • · • 4 4 4 4 4 « 4 4 « • 9 4 9 9 • • '9 • · 44 ··· 99 «·

Pro Asn Pro Val 100 Ala Gin Leu Met Ile 105 Ile Gly Trp Ala Phe 110 Ala Phe atg att gaa ggc gca tcc ggc ttc ggc acg cct gcc gcg att gcc gcg 384 Met Ile Glu 115 Gly Ala Ser Gly Phe 120 Gly Thr Pro Ala Ala 125 Ile Ala Ala ccg att ctg atg agc ttg ggc ttc aac ccg ttg aaa gtg gcg att ttc 432 Pro Ile 130 Leu Met Ser Leu Gly 135 Phe Asn Pro Leu Lys 140 Val Ala Ile Phe act ttg gtg atg aac tcc gtc ccc gtc tet ttc ggc gcg gtc ggt acg 480 Thr 145 Leu Val Met Asn Ser 150 Val Pro Val Ser Phe 155 Gly Ala Val Gly Thr 160 ccg acc tgg ttc ggt ttc gca ccg ctg aac ctg agt gcc gaa gac atc 528 Pro Thr Trp Phe Gly 165 Phe Ala Pro Leu Asn 170 Leu Ser Ala Glu Asp 175 Ile ctc gcc atc ggc agg cag acc ggc gta atg cac ttc ttc gca ggt ttc 576 Leu Ala Ile Gly 180 Arg Gin Thr Gly Val 185 Met His Phe Phe Ala 190 Gly Phe gtc atc ccc gtc atc ggc ttg ggc ttc atc gta cct tgg tet gaa atc 624 Val Ile Pro 195 Val Ile Gly Leu Gly 200 Phe Ile Val Pro Trp 205 Ser Glu Ile cgc aaa aac ttg ggc ttc gtc gcc att gcc gtc ttc tcc tgc acc att 672 Arg Lys 210 Asn Leu Gly Phe Val 215 Ala Ile Ala Val Phe 220 Ser Cys Thr Ile cct tat gtc gca ttg gcg atg gtc aac gaa gaa ttc ccg tcg ctc gtc 720 Pro 225 Tyr Val Ala Leu Ala 230 Met Val Asn Glu Glu 235 Phe Pro Ser Leu Val 240 gcc ggc gcg atc ggc ctg atg gtg tcc gta ttc gcc gcc aac caa ggc 768 Ala Gly Ala Ile Gly 245 Leu Met Val Ser Val 250 Phe Ala Ala Asn Gin 255 Gly tgg ggc ttg agc aaa gac -cac gcc aaa gac ccg aat gcc gaa aaa gtg 816 Trp Gly Leu Ser 260 Lys Asp His Ala Lys 265 Asp Pro Asn Ala Glu 270 Lys Val ccg ttc gcc caa gtc gcc aaa gca ctc gcc cct ttg ggt atg ctg atc 864 Pro Phe Ala 275 Gin Val Ala Lys Ala 280 Leu Ala Pro Leu Gly 285 Met Leu Ile ggc atg ctg gtg gtt acg cgc atc aaa cag ctc ggc atc aaa ggc att 912

175

WO 01/85772 PCT/GBO1/02003 • · · · · ······ e β · to 0·«· ·· · • ·· ·· « · · • ··«· · · · · · β to · · to · to to to • to·· ···· ·· ··· ·· ··

Gly Met 290 Leu Val Val Thr Arg 295 Ile Lys Gin Leu Gly 300 Ile Lys Gly Ile ttg acc agc aaa gaa gaa tgg ttc agc ttc caa ctg ccg ttt gat ttg 960 Leu 305 Thr Ser Lys Glu Glu 310 Trp Phe Ser Phe Gin 315 Leu Pro Phe Asp Leu 320 tcc aaa atc acc gtc agc gac tcc ctg acg att act ttc ggc aat att 1008 Ser Lys Xle Thr Val 325 Ser Asp Ser Leu Thr 330 Ile Thr Phe Gly Asn 335 Ile ttc gga caa gat gtc agc gcg tet tac caa acg ctg tac gtc ccg gct 1056 Phe Gly Gin Asp 340 Val Ser Ala Ser Tyr 345 Gin Thr Leu Tyr Val 350 Pro Ala tgg att ccg ttt gtg ctg acc gtt tgg att tgc atc ctg ctg tat aaa 1104 Trp Xle Pro 355 Phe Val Leu Thr Val 360 Trp Ile Cys Ile Leu 365 Leu Tyr Lys acc aaa ttc aaa gat gcc tgg acg att tat tcc gta act ttc aat caa 1152 Thr Lys 370 Phe Lys Asp Ala Trp 375 Thr Ile Tyr Ser Val 380 Thr Phe Asn Gin acc aaa aaa ccg ctg ctt gcc ctg atg ggc gcg ctg att atg gtt cag 1200 Thr 385 Lys Lys Pro Leu Leu 390 Ala Leu Met Gly Ala 395 Leu Ile Met Val Gin 400 ctg atg ctg gtc ggc ggc gac aat tcg atg gtg aaa atc atc ggt aag 1248 Leu Met Leu Val Gly 405 Gly Asp Asn Ser Met 410 Val Lys Ile Ile Gly 415 Lys gaa ttt gcc gca atg gcg ggc gaa cac tgg gtt tat ttc tcg ccg tat 1296 Glu Phe Ala Ala 420 Met Ala Gly Glu His 425 Trp Val Tyr Phe Ser 430 Pro Tyr ctg ggc gcg atc ggt gcg ttc ttc tcc ggt tcc aac acc gtg tcc aac 1344 Leu Gly Ala 435 Ile Gly Ala Phe Phe 440 Ser Gly Ser Asn Thr 445 Val Ser Asn ctg acc ttc ggc ccg att' 'cag cag caa atc gcc ttg gat acc ggc ctg 1392 Leu Thr 450 Phe Gly Pro Ile Gin 455 Gin Gin Ile Ala Leu 460 Asp Thr Gly Leu tcc gtt acc ctg att ctg gcg ttg cag tcc gtc ggc ggc gcg atg ggc 1440 Ser 465 Val Thr Leu Ile Leu 470 Ala Leu Gin Ser Val 475 Gly Gly Ala Met Gly 480 aat atg gtg tgc ctc aac aac atc atc gcc gta tgt acc gta ttg gat 1488

176

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Asn Met Val Cys Leu 485 Asn Asn Ile gtg aaa aat tcc gaa ggt gcg att Val Lys Asn Ser 500 Glu Gly Ala Ile gcg att tac ggc gtg att gcc gtc Ala Ile Tyr 515 Gly Val Ile Ala Val 520

Ile Ala 490 Val Cys Thr Val Leu 495 Asp atc aag aaa acc gtt atc ccg atg Ile 505 Lys Lys Thr Val Ile 510 Pro Met gtc gcg gca atg att ttc ttc ctc Val Ala Ala Met Ile 525 Phe Phe Leu

1536

1584 <210> 90 <211> 528 <212> PRT

<213> Neisseria meningitidis ' <400> 90 Met Ala Leu Phe Leu Ser Ile Phe Pro Ile Val Leu Leu Ile Trp Leu 1 5 10 15 Met Val Lys Lys Asn Ser Met Pro Ser Tyr Val Ala Leu Pro Ile Thr 20 25 30 Ala Val Leu Ile Tyr Ala Ile Lys Leu Phe Tyr Phe Asp Asp Ala Gly 35 40 45 Met Leu Leu Asn Ala Thr Ala Ala Ser Gly Leu Val Lys Thr Leu Thr 50 55 60 Pro Ile Thr Val Ile Phe Gly Ala Ile Met Phe Asn Arg Met Met Glu ⁶⁵ 70 75 80 Thr Thr Gly Cys Ile Asp Val Ile Arg Lys Trp Leu Ala Thr Ile Ser 85 90 95 Pro Asn Pro Val Ala Gin Leu Met Ile Ile Gly Trp Ala Phe Ala Phe 100 105 110 Met Ile Glu Gly Ala Ser* Gly Phe Gly Thr Pro Ala Ala Ile Ala Ala 115 120 125 Pro Ile Leu Met Ser Leu Gly Phe Asn Pro Leu Lys Val Ala Ile Phe 130 135 140 Thr Leu Val Met Asn Ser Val Pro Val Ser Phe Gly Ala Val Gly Thr 145 150 155 160

177 ·,« ·· ·· · ·· ···· ···· · · · · · · · • · · · · ···

9 · · · · 4 · · » ···· ···· ·· ··· 94 *·

WO 01/85772 PCT/GB01/02003

Pro Thr Trp Phe Gly 165 Phe Ala Pro Leu Asn 170 Leu Ser Ala Glu Asp 175 Ile Leu Ala Ile Gly 180 Arg Gin Thr Gly Val 185 Met His Phe Phe Ala 190 Gly Phe Val Xle Pro 195 Val Ile Gly Leu Glý 200 Phe Ile Val Pro Trp 205 Ser Glu Ile Arg Lys 210 Asn Leu Gly Phe Val 215 Ala Ile Ala Val Phe 220 Ser Cys Thr Ile Pro 225 Tyr Val Ala Leu Ala 230 Met Val Asn Glu Glu 235 Phe Pro Ser Leu Val 240 Ala Gly Ala Ile Gly 245 Leu Met vál Ser Val 250 Phe Ala Ala Asn Gin 255 Gly Trp Gly Leu Ser 260 Lys Asp His Ala Lys 265 Asp Pro Asn Ala Glu 270 Lys Val Pro Phe Ala 275 Gin Val Ala Lys Ala 280 Leu Ala Pro Leu Gly 285 Met Leu Ile Gly Met 290 Leu Val Val Thr Arg 295 Ile Lys Gin Leu Gly 300 Ile Lys Gly Ile Leu 305 Thr Ser Lys Glu Glu 310 Trp Phe Ser Phe Gin 315 Leu Pro Phe Asp Leu 320 Ser Lys Ile Thr Val 325 Ser Asp Ser Leu Thr 330 Ile Thr Phe Gly Asn 335 Ile Phe Gly Gin Asp 340 Val Ser Ala Ser Tyr 345 Gin Thr Leu Tyr Val 350 Pro Ala Trp Ile Pro 355 Phe Val Leu Thr Val 360 Trp Ile Cys Ile Leu 365 Leu Tyr Lys Thr Lys 370 Phe Lys Asp Ala Trp 375 Thr Ile Tyr Ser Val 380 Thr Phe Asn Gin Thr 385 Lys Lys Pro Leu Leu 390 Ala Leu Met Gly Ala 395 Leu Ile Met Val Gin 400 Leu Met Leu Val Gly 405 Gly Asp Asn Ser Met 410 Val Lys Ile Ile Gly 415 Lys

178

WO 01/85772 PCT/GB01/02003

Glu Phe Ala Ala Met Ala Gly Glu His Trp Val Tyr Phe Ser Pro Tyr 420 425 430 Leu Gly Ala Ile Gly Ala Phe Phe Ser Gly Ser Asn Thr Val Ser Asn 435 440 - 445 Leu Thr Phe Gly Pro Ile Gin Gin Gin Ile Ala Leu Asp Thr Gly Leu 450 455 460 Ser Val Thr Leu Ile Leu Ala Leu Gin Ser Val Gly Gly Ala Met Gly 465 470 475 480 Asn Met Val Cys Leu Asn Asn Ile Ile Ala Val Cys Thr Val Leu Asp 485 490 495 Val Lys Asn Ser Glu Gly Ala Ile Ile Lys Lvs Thr Val Ile Pro Met 500 505 510 Ala Ile Tyr Gly Val Ile Ala Val Val Ala Ala Met Ile Phe Phe Leu 515 520 525

<210> 91 <211> 1686 <212> DNA <213> Neisseria meningitidis <22 0>

<221> CDS <222> (1) . . (1686) <400> 91 atg tta caa tcc gaa aat tcg aaa aat tta atc tet tgt tea ata aag 48

Met Leu Gin Ser Glu Asn Ser Lys Asn Leu Ile Ser Cys Ser Ile Lys

1 5 10 15 gct tta cca atc atg att tet acc aac ggc atc acc atg cag ttc ggc 96

Ala Leu Pro Ile Met Ile Ser Thr Asn Gly Ile Thr Met Gin Phe Gly

20 25 30 gcg aag ccg ctg ttt gaa aac gta tcc gtt aaa ttc ggc gaa ggc aac 144

Ala Lys Pro Leu Phe Glu Asn Val Ser Val Lys Phe Gly Glu Gly Asn

35 40 45 cgc tac ggt ttg atc ggc gcg aac ggc tea ggc aaa tcc acc ttc atg 192

Arg Tyr Gly Leu Ile Gly Ala Asn Gly Ser Gly Lys Ser Thr Phe Met

50 55 60

179

• 0 «-· ♦ · 0 00 0000 « β β β 0 0 0 0 β 0 0 0 0 0 0 0 ί» • 0 ··» «0 0«

WO 01/85772

PCT/GB01/02003

aaa atc ctc ggc ggc gat ttg gaa cag aca gcc ggc gaa gtg gcg att 240 Lys 65 Ile Leu Gly Gly Asp 70 Leu Glu Gin Thr Ala 75 Gly Glu Val Ala Ile 80 gaa aac ggc gtg cgt ttg ggt aaa ttg cgc caa gac cag ttt gcc tac 288 Glu Asn Gly Val Arg Leu Gly Lys Leu Arg Gin Asp Gin Phe Ala Tyr 85 90 95 gaa gat atg cgc gtg ctg gac gtg gta atg atg ggt cac acc gaa atg 336 Glu Asp Met Arg Val Leu Asp Val Val Met Met Gly His Thr Glu Met 100 105 110 tgg gcg gcg atg acc gaa cgt gat gcg att tac gcc aat ccc gaa gcc 384 Trp Ala Ala Met Thr Glu Arg Asp Ala Ile Tyr Ala Asn Pro Glu Ala 115 120 125 acc gaa gac gac tac atg aaa gcc gca gaa ctg gaa gcc aag ttc gcc 432 Thr Glu Asp Asp Tyr Met Lys Ala Ala Glu Leu Glu Ala Lys Phe Ala 130 135 140 gaa tac gac ggc tac acc gcc gaa gcg cgt gcc gcc gag ttg ttg agc 480 Glu Tyr Asp Gly Tyr Thr Ala Glu Ala Arg Ala Ala Glu Leu Leu Ser 145 150 155 160 ggc gtg ggc att tcc gaa gat ttg cac aat gca acc atg gca gaa gtt 528 Gly Val Gly Ile Ser Glu Asp Leu His Asn Ala Thr Met Ala Glu Val 165 170 175 gcc ccg ggc ttc aaa ctg cgc gta ttg ctg gcg caa gcc ctg ttc tcc 576 Ala Pro Gly Phe Lys Leu Arg Val Leu Leu Ala Gin Ala Leu Phe Ser 180 185 190 aag ccg gat gta ttg ctc ttg gac gaa ccg acc aat aac ttg gac att 624 Lys Pro Asp Val Leu Leu Leu Asp Glu Pro Thr Asn Asn Leu Asp Ile 195 200 205 aat acc atc cgt tgg ttg gaa ggc gtg ttg aac cag tac gac tcc acg 672 Asn Thr Ile Arg Trp Leu Glu Gly Val Leu Asn Gin Tyr Asp Ser Thr 210 215 220 atg att atc atc tcg cac gac cgc cac ttc ttg aac gaa gtc tgc acg 720 Met Ile Ile Ile Ser His Asp Arg His Phe Leu Asn 'Glu Val Cys Thr 225 230 235 240 cat atg gca gat ttg gat tac aac acc atc acc atc tat ccg ggc aac 768 His Met Ala Asp Leu Asp Tyr Asn Thr Ile Thr Ile Tyr Pro Gly Asn 245 250 255

180

WO 01/85772

PCT/GB01/02003

tac gac gac tat atg Met ctc Leu gcc Ala tcc gcc caa tcg cgc gaa cgc gcc ctg 816 Tyr Asp Asp Tyr 260 Ser Ala 265 Gin Ser Arg Glu Arg 270 Ala Leu aaa gac aat gcc aag gcg aag gaa aaa ttg cag gaa ttg caa gaa ttt 864 Lys Asp Asn Ala Lys Ala Lys Glu Lys Leu Gin Glu Leu Gin Glu Phe 275 280 285 gtg gcg cgg ttc agt gcc aac aaa tcc aaa gcc cgt cag gca acc agc 912 Val Ala Arg Phe Ser Ala Asn Lys Ser Lys Ala Arg Gin Ala Thr Ser 290 295 300 cgt ctg aaa cag gca gac aaa atc aaa tcg gag atg gtc gag gtc aaa 960 Arg Leu Lys Gin Ala Asp Lys Ile Lys Ser Glu Met Val Glu Val Lys 305 310 315 320 ccc tet acc cgt caa aac ccg tat atc cgt ttt gaa gcc gat gaa aaa 1008 Pro Ser Thr Arg Gin Asn Pro Tyr Ile Arg Phe Glu Ala Asp Glu Lys 325 330 335 gcc aag ctg cac cgt cag gct gtg gaa gtt gaa aaa ctg gcg aaa cgc 1056 Ala Lys Leu His Arg Gin Ala Val Glu Val GlU Lys Leu Ala Lys Arg 340 345 350 ttt gaa acc cag ttg ttt aaa aac ctg aac ttt atc ctt gaa gcg gga 1104 Phe Glu Thr Gin Leu Phe Lys Asn Leu Asn Phe Ile Leu Glu Ala Gly 355 360 365 caa cgc ctc gcc atc atc ggc ccg aac gg<= gcg ggc aaa tcc acc ctg 1152 Gin Arg Leu Ala Ile Ile Gly Pro Asn Gly Ala Gly Lys Ser Thr Leu 370 375 380 ctg aaa ctc ttg gcc ggc gcg tac aac ccc gaa tat tca gac ggt ctg 1200 Leu Lys Leu Leu Ala Gly Ala Tyr Asn Pro Glu Tyr Ser Asp Gly Leu 385 390 395 400 ctg ccg gac gaa ggc agc atc aaa tgg gcg gaa aaa gcc agc gtc ggc 1248 Leu Pro Asp Glu Gly Ser Ile Lys Trp Ala Glu Lys Ala Ser Val Gly 405 410 415 tac tat ccg caa gat cac gaa aac gac ttc gat gtc gat atg gat ttg 1296 Tyr Tyr Pro Gin Asp His Glu Asn Asp Phe Asp Val Asp Met Asp Leu 420 425 430 agc gaa tgg atg cgc caa tgg ggg cag gac ggc gac gac gaa caa gtc 1344 Ser Glu Trp Met Arg Gin Trp Gly Gin Asp Gly Asp Asp Glu Gin Val 435 440 445

181

WO 01/85772 PCT/GB01/02003 ·'·

atc cgc ggc act ttg ggg cgt ttg ctc ttc ggc agt aac gat gtc gtg 1392 Ile Arg 450 Gly Thr Leu Gly Arg 455 Leu Leu Phe Gly Ser 460 Asn Asp Val Val aaa aaa gtg aag gtt ctc tcc ggt ggt gaa aaa ggc cgt atg ctt tac 1440 Lys Lys Val Lys Val Leu Ser Gly Gly Glu Lys Gly Arg Met Leu Tyr 465 470 475 480 ggc aaa ctg ttg ctg ttg aaa ccc aat gtc tta gtc atg gat gaa ccg 1488 Gly Lys Leu Leu Leu Leu Lys Pro Asn Val Leu Val Met Asp Glu Pro 485 490 495 acc aac cac atg gac atg gaa agc atc gaa tcc ttg aac atg gcg ctg 1536 Thr Asn His Met Asp Met Glu Ser Ile Glu Ser Leu Asn Met Ala Leu 500 505 510 gaa aaa tac aac ggc acg ctg att ttc gtc tcc cac gac cgt cag ttc 1584 Glu Lys Tyr Asn Gly Thr Leu Ile Phe Val Ser His Asp Arg Gin Phe 515 520 525 gtt tet tcc ctg gct acc caa atc att gaa ttg gac ggc aaa ggc gga 1632 Val Ser Ser Leu Ala Thr Gin Ile Ile GlU Leu Asp Gly Lys Gly Gly 530 535 540 tat gaa cac tac ttg ggc gat tac gaa agc tat ctc gag aaa aaa ggc 1680 Tyr Glu His Tyr Leu Gly Asp Tyr Glu Ser Tyr Leu Glu Lys Lys Gly 545 550 555 560

gta gca 1686

Val Ala <210> 92 <211> 562 <212> PRT <213> Neisseria meningitidis <400> 92

Met Leu Gin Ser Glu Asň Ser Lys Asn Leu Ile Ser Cys Ser Ile Lys 1 5 10 15 Ala Leu Pro Ile Met Ile Ser Thr Asn Gly Ile Thr Met Gin Phe Gly 20 25 30 Ala Lys Pro Leu Phe Glu Asn Val Ser Val Lys Phe Gly Glu Gly Asn

35 40 45

182

WO 01/85772 PCT/GB01/02003 Arg Tyr Gly Leu Ile Gly Ala Asn 50 55 Gly Ser Gly Lys Ser Thr Phe Met 60 Lys Ile Leu Gly Gly Asp Leu Glu 65 70 Gin Thr Ala Gly Glu Val Ala Ile 75 80 Glu Asn Gly Val Arg Leu Gly Lys 85 Leu Arg Gin Asp Gin Phe Ala Tyr 90 95 Glu Asp Met Arg Val Leu Asp Val 100 Val Met Met Gly His Thr Glu Met 105 110 Trp Ala Ala Met Thr Glu Arg Asp 115 120 Ala Ile Tyr Ala Asn Pro Glu Ala 125 Thr Glu Asp Asp Tyr Met Lys Ala 130 135 Ala Glu Leu Glu Ala Lys Phe Ala 140 Glu Tyr Asp Gly Tyr Thr Ala Glu 145 150 Ala Arg Ala Ala Glu Leu Leu Ser 155 160 Gly Val Gly Ile Ser Glu Asp Leu 165 His Asn Ala Thr Met Ala Glu Val 170 175 Ala Pro Gly Phe Lys Leu Arg Val 180 Leu Leu Ala Gin Ala Leu Phe Ser 185 190 Lys Pro Asp Val Leu Leu Leu Asp 195 200 Glu Pro Thr Asn Asn Leu Asp Ile 205 Asn Thr Ile Arg Trp Leu Glu Gly 210 215 Val Leu Asn Gin Tyr Asp Ser Thr 220 Met Ile Ile Ile Ser His Asp Arg 225 230 His Phe Leu Asn Glu Val Cys Thr 235 240 His Met Ala Asp Leu Asp Tyr Asn 245 Thr Ile Thr Ile Tyr Pro Gly Asn 250 255 Tyr Asp Asp Tyr Met Leu*Ala Ser 260 Ala Gin Ser Arg Glu Arg Ala Leu 265 270 Lys Asp Asn Ala Lys Ala Lys Glu 275 280 Lys Leu Gin Glu Leu Gin Glu Phe 285 Val Ala Arg Phe Ser Ala Asn Lys 290 295 Ser Lys Ala Arg Gin Ala Thr Ser 300

183

' · • • · • · • « • · · • * · · · • • · • ·· ···· 4 4 · 4 44 494 WO 01/85772 PCT/GB01/02003 Arg Leu Lys Gin Ala Asp Lys Ile Lys Ser Glu Met Val Glu Val Lys 305 310 315 320 Pro Ser Thr Arg Gin Asn Pro Tyr Ile Arg Phe Glu Ala Asp Glu Lys 325 330 335 Ala Lys Leu His Arg Gin Ala Val Glu Val Glu Lys Leu Ala Lys Arg 340 345 350 Phe Glu Thr Gin Leu Phe Lys Asn Leu Asn Phe Ile Leu Glu Ala Gly 355 360 365 Gin Arg Leu Ala Ile Ile Gly Pro Asn Gly Ala Gly Lys Ser Thr Leu 370 375 380 Leu Lys Leu Leu Ala Gly Ala Tyr Asn Pro Glu Tyr Ser Asp Gly Leu 385 390 395 400 Leu Pro Asp Glu Gly Ser Ile Lys Trp Ala Glu Lys Ala Ser Val Gly 405 410 415 Tyr Tyr Pro Gin Asp His Glu Asn Asp Phe Asp Val Asp Met Asp Leu 420 425 430 Ser Glu Trp Met Arg Gin Trp Gly Gin Asp Gly Asp Asp Glu Gin Val 435 440 445 Xle Arg Gly Thr Leu Gly Arg Leu Leu Phe Gly Ser Asn Asp Val Val 450 455 460 Lys Lys Val Lys Val Leu Ser Gly Gly Glu Lys Gly Arg Met Leu Tyr 465 470 475 480 Gly Lys Leu Leu Leu Leu Lys Pro Asn Val Leu Val Met Asp Glu Pro 485 490 495 Thr Asn His Met Asp Met Glu Ser Ile Glu Ser Leu Asn Met Ala Leu 500 505 510 Glu Lys Tyr Asn Gly Thr Leu Ile Phe Val Ser His Asp Arg Gin Phe 515 520 525 Val Ser Ser Leu Ala Thr Gin Ile Ile Glu Leu Asp Gly Lys Gly Gly 530 535 540 Tyr Glu His Tyr Leu Gly Asp Tyr Glu Ser Tyr Leu Glu Lys Lys Gly 545 550 555 560

184 • ··· ···· • »·

WO 01/85772 PCT/GBO1/O2OO3

Val Ala <210> 93 <211> 1126 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1125) <400> 93

atg Met 1 aac acc tcg caa cgc Arg aac cgc ctc gtc age cgc tgg ctc aac tec 48 Asn Thr Ser Gin 5 Asn Arg Leu Val 10 Ser Arg Trp Leu Asn 15 Ser tac gaa cgc tac cgc tac cgc cgc ctc atc cac gcc gtc cgg ctc ggc 96 Tyr Glu Arg Tyr Arg Tyr Arg Arg Leu Ile His Ala Val Arg Leu Gly 20 25 30 ggg gcc gtc ctg ttc gcc acc gcc tcc gcc cgg ctg ctc cac ctc caa 144 Gly Ala Val Leu Phe Ala Thr Ala Ser Ala Arg Leu Leu His Leu Gin 35 40 45 cac ggc gag tgg ata ggg atg acc gtc ttc gtc gtc ctc ggc atg ctc 192 His Gly Glu Trp Ile Gly Met Thr Val Phe Val Val Leu Gly Met Leu 50 55 60 cag ttt caa ggg gcg att tac tcc aag gcg gtg gaa cgt atg ctc ggc 240 Gin Phe Gin Gly Ala Ile Tyr Ser Lys Ala Val Glu Arg Met Leu Gly 65 70 75 80 acg gtc atc ggg ctg ggc gcg ggt ttg ggc gtt tta tgg ctg aac cag 288 Thr Val Ile Gly Leu Gly Ala Gly Leu Gly Val Leu Trp Leu Asn Gin 85 90 95 cat tat ttc cac ggc aacj ctc ctc ttc tac ctc acc gtc ggc acg gca 336 His Tyr Phe His Gly Asn Leu Leu Phe Tyr Leu Thr Val Gly Thr Ala 100 105 110 age gca ctg gcc ggc tgg gcg gcg gtc ggc aaa aac ggc tac gtc cct 384 Ser Ala Leu Ala Gly Trp Ala Ala Val Gly Lys Asn Gly Tyr Val Pro 115 120 125 atg ctg gcg ggg ctg acg atg tgc atg ctc atc ggc gac aac ggc age 432

185 *1

WO 01/85772 PCT/GB01/02003 Met Leu 130 Ala Gly Leu Thr Met 135 Cys Met Leu Ile Gly Asp 140 Asn Gly Ser gaa tgg ttc gac age ggc ctg atg cgc gcg atg aac gtc ctc atc ggc 480 Glu 145 Trp Phe Asp Ser Gly 150 Leu Met Arg Ala Met 155 Asn Val Leu Ile Gly 160 gcg gcc atc gcc atc gcc gcc gcc aaa ctg ctg ccg ctg aaa tcc aca 528 Ala Ala Ile Ala Ile 165 Ala Ala Ala Lys Leu 170 Leu Pro Leu Lys Ser 175 Thr ctg atg tgg cgt ttc atg ctt gcc gac aac ctg acc gac tgc age aaa 576 Leu Met Trp Arg 180 Phe Met Leu Ala Asp 185 Asn Leu Thr Asp Cys 190 Ser Lys atg att gcc gaa atc age aac ggc agg cgc atg acc cgc gaa cgc ctc 624 Met Ile Ala 195 Glu Ile Ser Asn Gly 200 Arg Arg Met Thr Arg 205 Glu Arg Leu gaa gag aac atg gcg aaa atg cgc caa atc aac gca cgc atg gtc aaa 672 Glu Glu 210 Asn Met Ala Lys Met 215 Arg Gin Ile Asn Ala 220 Arg Met Val Lys age cgc age cac ctc gcc gcc aca tcg ggc gaa age cgc atc age ccc 720 Ser 225 Arg Ser l His Leu Ala 230 Ala Thr Ser Gly Glu 235 Ser Arg Ile Ser Pro 240 gcc atg atg gaa gcc atg cag cac gcc cac cgt aaa att gtc aac acc 768 Ala Met Met Glu Ala 245 Met Gin His Ala His 250 Arg Lys Ile Val Asn 255 Thr acc gag ctg ctc ctg acc acc gcc gcc aag ctg caa tet ccc aaa ctc 816 Thr Glu Leu Leu 260 Leu Thr Thr Ala Ala 265 Lys Leu Gin Ser Pro 270 Lys Leu aac ggc age gaa atc cgg ctg ctt gac cgc cac ttc aca Ctg ctc caa 864 Asn Gly Ser 275 Glu Ile Arg Leu Leu 280 Asp Arg His Phe Thr 285 Leu Leu Gin acc gac ctg caa caa acc gtc gcc ctt atc aac ggc aga cac gcc cgc 912 Thr Asp 290 Leu Gin Gin Thr Val 295 Ala Leu Ile Asn Gly Arg 300 His Ala Arg cgc atc cgc atc gac acc gcc atc aac ccc gaa ctg gaa gcc ctc gcc 960 Arg 305 Ile Arg Ile Asp Thr 310 Ala Ile Asn Pro Glu 315 Leu Glu Ala Leu Ala 320 gaa cac ctc cac tac caa tgg cag ggc ttc ctc tgg ctc age acc aat 1008

-l·

186 • · 4 « ·«·· » • · · · 4 · ·

WO 01/85772 PCT/GB01/02003

Glu His Leu His Tyr Gin Trp Gin Gly Phe Leu Trp Leu Ser Thr Asn 325 330 335 atg cgt cag gaa att tcc gcc ctc gtc atc ctg ctg caa cgc acc cgc 1056 Met Arg Gin Glu Xle Ser Ala Leu Val Ile Leu Leu Gin Arg Thr Arg 340 345 350 cgc aaa tgg ctg gat gcc cac gaa cgc caa cac ctg cgc caa age ctg 1104 Arg Lys Trp Leu Asp Ala His Glu Arg Gin His Leu Arg Gin Ser Leu 355 360 365 ctt gaa aca cgg gaa cac agt t 1126 Leu Glu Thr Arg Glu His Ser 370 375

<210> 94 <211> 375 <212> PRT <213> Neisseria meningitidis <400> 94

Met 1 Asn Thr Ser Gin 5 Arg Asn Arg Leu Val 10 Ser Arg Trp Leu Asn 15 Ser Tyr Glu Arg Tyr 20 Arg Tyr Arg Arg Leu 25 Ile His Ala Val Arg 30 Leu Gly Gly Ala Val 35 Leu Phe Ala Thr Ala 40 Ser Ala Arg Leu Leu 45 His Leu Gin His Gly 50 Glu Trp Ile Gly Met 55 Thr Val Phe Val Val 60 Leu Gly Met Leu Gin 65 Phe Gin Gly Ala Ile 70 Tyr Ser Lys Ala Val 75 Glu Arg Met Leu Gly 80 Thr Val Ile Gly Leu 85 Gly Ala Gly Leu Gly 90 Val Leu Trp Leu Asn 95 Gin His Tyr Phe His 100 Gly Asn Leu Leu Phe 105 Tyr Leu Thr Val Gly 110 Thr Ala Ser Ala Leu 115 Ala Gly Trp Ala Ala 120 Val Gly Lys Asn Gly 125 Tyr Val Pro Met Leu Ala Gly Leu Thr Met Cys Met Leu Ile Gly Asp Asn Gly Ser

130 135 140

187 « · · • · · ··

WO Ot/85772 PCT/GB01/02003 Glu Trp Phe Asp Ser Gly Leu 145 150 Met Arg Ala Met Asn Val Leu Ile Gly 155 160 Ala Ala Ile Ala Ile Ala Ala 165 Ala Lys Leu Leu Pro Leu Lys Ser Thr 170 175 Leu Met Trp Arg Phe Met Leu 180 Ala Asp Asn Leu Thr Asp Cys Ser Lys 185 190 Met Ile Ala Glu Ile Ser Asn 195 Gly Arg Arg Met Thr Arg Glu Arg Leu 200 205 Glu Glu Asn Met Ala Lys Met 210 215 Arg Gin Ile Asn Ala Arg Met Val Lys. 220 Ser Arg Ser His Leu Ala Ala 225 230 Thr Ser Gly Glu Ser Arg Ile Ser Pro 235 240 Ala Met Met Glu Ala Met Gin 245 His Ala His Arg Lys Ile Val Asn Thr 250 255 Thr Glu Leu Leu Leu Thr Thr 260 Ala Ala Lys Leu Gin Ser Pro Lys Leu 265 270 Asn Gly Ser Glu Ile Arg Leu ’ 275 Leu Asp Arg His Phe Thr Leu Leu Gin 280 285 Thr Asp Leu Gin Gin. Thr Val 290 295 Ala Leu Ile Asn Gly Arg His Ala Arg 300 Arg Ile Arg Ile Asp Thr Ala 305 310 Ile Asn Pro Glu Leu Glu Ala Leu Ala 315 320 Glu His Leu His Tyr Gin Trp 325 Gin Gly Phe Leu Trp Leu Ser Thr Asn 330 335 Met Arg Gin Glu Ile Ser Ala 340 Leu Val Ile Leu Leu Gin Arg Thr Arg 345 350 Arg Lys Trp Leu Asp Ala His 355 Leu Glu Thr Arg Glu His Ser 370 375 Glu Arg Gin His Leu Arg Gin Ser Leu 360 365

188 • # · 9 9 9 ® ® < · • · · » · 9 9 9

WO 01/85772 PCT/GBO1/O20O3 <210> 95 <211> 1725· <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1725) <400> 95

atg Met 1 cag cta tea ggc gcg caa atc Gin Ile ata Ile gtg cag agt ctc aaa gcc gaa 48 Gin Leu Ser Gly 5 Ala Val 10 Gin Ser Leu Lys Ala 15 Glu ggt gtc gag tac gtt ttc ggt tat ccc ggc ggt gcg gtt atc gaa atc 96 Gly Val Glu Tyr Val Phe Gly Tyr Pro Gly Gly Ala Val Ile Glu Ile 20 25 30 tac gat gcc ctt ttc caa ctc aat aaa ttc aag cac att ctg acc cgt 144 Tyr Asp Ala Leu Phe Gin Leu Asn Lys Phe Lys His Ile Leu Thr Arg 35 40 45 cac gag cag gcg gca gta cac gcg gca gat gcg tat gcg cgc gtc age 192 His Glu Gin Ala Ala Val His Ala Ala Asp Ala Tyr Ala Arg Val Ser 50 55 60 ggt aag gtg ggc gtg gca ttg gtt aca tcc ggc ccg ggc gtt acc aat 240 Gly Lys Val Gly Val Ala Leu Val Thr Ser Gly Pro Gly Val Thr Asn 65 70 75 80 gca ctg acc ggt att get act gcc tat acg gat tcg att ccg atg gtg 288 Ala Leu Thr Gly Ile Ala Thr Ala Tyr Thr Asp Ser Ile Pro Met Val 85 90 95 gtc atc age ggg cag gta ggc aat tcc ctg att ggt acg gat gcg ttc 336 Val Ile Ser Gly Gin val Gly Asn Ser Leu Ile Gly Thr Asp Ala Phe 100 1Ů5 110 caa gaa gtt gat acg gtg ggt att acc cgt ccg tgc gtc aaa cac aat 384 Gin Glu Val Asp Thr Val_: .Gly Ile Thr Arg Pro Cys Val Lys His Asn 115 120 125 ttc ctg gtt acg gac atc aat gag ttg gcg gaa acc att aaa aag gcg 432 Phe Leu Val Thr Asp Ile Asn Glu Leu Ala Glu Thr Ile Lys Lys Ala 130 135 140 ttc caa att gcc gca age ggc ega ccg ggg ccc gtg gtg gtt gat gtc 480 Phe Gin Ile Ala Ala Ser Gly Arg Pro Gly Pro Val Val Val Asp Val

189

WO 01/85772 PCT/GB01/02003

·« • 9 ·♦ 94 4 4 4 9 9 4 4 9 9 4 4 9444 4 9 4 4 9 9 4 4 4 9 4 9 4 4 4 · 4 4 · ···· 49 9 444 4 44 4 4 4 4 4

145 150 155 160 ccg aaa gat gtt acg cag gcg atg gcg aaa ttc age tat cct cag gaa 528 Pro Lys Asp Val Thr Gin Ala Met Ala Lys Phe Ser Tyr Pro Gin Glu 165 170 175 gac att ttt atc cgt tcg tat caa ccg gtt gtt caa ggg cat atc ggt 576 Asp Ile Phe Ile Arg Ser Tyr Gin Pro Val Val Gin Gly His Ile Giy 180 ¹⁸⁵ 190 cag att aaa aag gcc gtg cag atg ttg gca tet gcc aaa cgt ccg gtc 624 Gin Ile Lys Lys Ala val Gin Met Leu Ala Ser Ala Lys Arg Pro Val 195 200 205 gtc tat ttt ggc ggc ggc gtg gtg ttg ggt aat get tet gaa gag ctg 672 Val Tyr Phe Gly Gly Gly Val Val Leu Gly Asn Ala Ser Glu Glu Leu 210 215 220 acc ega ttt gtc ega atg acg ggt get ccg tgc acg ggt acg ctg atg 720 Thr Arg Phe Val Arg Met Thr Gly Ala Pro Cys Thr Gly Thr Leu Met 225 230 235 240 ggc ttg ggc get tat cct tcc ggc gac cgc caa ttc ctc ggt atg ctc 768 Gly Leu Gly Ala Tyr Pro Ser Gly Asp Arg Gin Phe Leu Gly Met Leu 245 250 255 ggt atg cac ggt act tac gag gca aac ctt gcc atg cag aat gcg gat 816 Gly Met His Gly Thr Tyr Glu Ala Asn Leu Ala Met Gin Asn Ala Asp 260 265 270 gtc gtt ctt gcc gta ggt gcg cgt ttt gac gac cgt gtg gtt tcc gta 864 Val Val Leu Ala Val Gly Ala Arg Phe Asp Asp Arg Val Val Ser Val 275 280 285 ccg tcc aaa ttt ttc gag aag. gcg aag aag gtt atc cat atc gat gtc 912 Pro Ser Lys Phe Phe Glu Lys Ala Lys Lys Val Ile His Ile Asp Val 290 295 300 gat cct tcc age atc gcc aaa cgc gtg aag gcg gac att ccg att gtc 960 Asp Pro Ser Ser Ile Ala 'Lys Arg Val Lys Ala Asp Ile Pro Ile Val 305 310 315 320 ggc gac gtg aaa aac gtt ttg tcc gag atg gtt gcg ctg tgg caa aaa 1008 Gly Asp Val Lys Asn Val Leu Ser Glu Met Val Ala Leu Trp Gin Lys 325 330 335 caa gag tcc gtg ccg tet gaa gat get ttg ggc aaa tgg tgg aaa acc 1056 Gin Glu Ser Val Pro Ser Glu Asp Ala Leu Gly Lys Trp Trp Lys Thr

190 fcfc fcfc fcfc · fcfcfcfc e © ee β • fcfc·· · • fcfcfc fc fcfcfc • · fcfcfc fc • fc

WO 01/85772 PCT/GBO1/02003 340 345 350 ata gag gaa tgg cgt tcc cgc gat tgc ttg tgg ttt gac aac ggc agc 1104 Ile Glu Glu Trp Arg Ser Arg Asp Cys Leu Trp Phe Asp Asn Gly Ser 355 360 365 gaa att atc aag cca caa tat gtg att cag aag ctt gcc gag att acc 1152 Glu Ile Ile Lys Pro Gin Tyr Val Ile Gin Lys Leu Ala Glu Ile Thr 370 375 380 ggc aat tcg gca atc atc aca tcg gat gta ggg cag cat caa atg ttt 1200 Gly Asn Ser Ala Ile Ile Thr Ser Asp Val Gly Gin His Gin Met Phe 385 390 395 400 gcg gct caa tat tat ccc ttc gaa cgt ccg cgc caa tgg ctc aac tcc 1248 Ala Ala Gin Tyr Tyr Pro Phe Glu Arg Pro Arg Gin Trp Leu Asn Ser 405 410 415 ggc ggt ttg ggt acg atg ggc gtt ggt ctg cct tat gcg att ggt gca 1296 Gly Gly Leu Gly Thr Met Gly Val Gly Leu Pro Tyr Ala Ile Gly Ala 420 425 430 aaa ctt gcc gcc ccg gat caa gac gta ttc tgt att acc ggc gac ggc 1344 Lys Leu Ala Ala Pro Asp Gin Asp val Phe Cys Ile Thr Gly Asp Gly 435 440 445 tcg atc cag atg aac atc caa gag ctg tcc acc tgt ttc caa tat cgg 1392 Ser Ile Gin Met Asn Ile Gin Glu Leu Ser Thr Cys Phe Gin Tyr Arg 450 455 460 att ccg gtt aac gtc att acg ctg aac aac ggt tat ctc ggt atg gta 1440 Ile Pro Val Asn Val Ile Thr Leu Asn Asn Gly Tyr Leu Gly Met Val 465 470 475 480 cgc cag tgg cag gaa ata tat tac ggc gga ega gag tcg gaa acc tat 1488 Arg Gin Trp Gin Glu Ile Tyr Tyr Gly Gly Arg Glu Ser Glu Thr Tyr 485 490 495 ttc gat tet ttg ccc gat ttc gtc aaa ctt gcc gag gca tac ggc cat 1536 Phe Asp Ser Leu Pro Asp Phe Val Lys Leu Ala Glu Ala Tyr Gly His 500 s 505 510 atc ggt atc cgc gtg gac aag aag tet gat gtg gaa ggt gcg ttg ttg 1584 Ile Gly Ile Arg Val Asp Lys Lys Ser Asp Val Glu Gly Ala Leu Leu 515 520 525 gaa gca ttg aac caa aaa gac agg ctg gtg ttt atc gac ttc ctg acc 1632 Glu Ala Leu Asn Gin Lys Asp Arg Leu Val Phe Ile Asp Phe Leu Thr

191

WO 01/85772

PCT/GB01/02003

530 gac cag aaa cag aat gtg Asp Gin Lys Gin Asn Val 545 550 gac gaa atg gta ctt ccg Asp Glu Met Val Leu Pro 565

535 540 atg ccc atg gtc ggc aac ggc aaa ggt ttg Met Pro Met Val Gly Asn Gly Lys Gly Leu 555 560 ccg cat atg cgt gcg gac gga aag gcg Pro His Met Arg Ala Asp Gly Lys Ala

1725

570

575 <210> 96 <211> 575 <212> PRT <213> Neisseria meningitidis <400> 96

Met 1 Gin Leu Ser Gly 5 Ala Gin Ile Ile Val 10 Gin Ser Leu Lys Ala 15 Glu Gly Val Glu Tyr 20 Val Phe Gly Tyr Pro 25 Gly Gly Ala Val Ile 30 Glu Ile Tyr Asp Ala 35 Leu Phe Gin Leu Asn 40 Lys Phe Lys His Ile 45 Leu Thr Arg His Glu 50 Gin Ala Ala Val His 55 Ala Ala Asp Ala Tyr 60 Ala Arg Val Ser Gly 65 Lys Val Gly Val Ala 70 Leu Val Thr Ser Gly 75 Pro Gly Val Thr Asn 80 Ala Leu Thr Gly Ile 85 Ala Thr Ala Tyr Thr 90 Asp Ser Ile Pro Met 95 Val Val Ile Ser Gly 100 Gin Val Gly Asn Ser 105 Leu Ile Gly Thr Asp 110 Ala Phe Gin Glu Val 115 Asp Thr Val Gly Ile 120 Thr Arg Pro Cys Val 125 Lys His Asn Phe Leu 130 Val Thr Asp Ile Asn 135 Glu Leu Ala Glu Thr 140 Ile Lys Lys Ala Phe 145 Gin Ile Ala Ala Ser 150 Gly Arg Pro Gly Pro 155 Val Val Val Asp Val 160 Pro Lys Asp Val Thr Gin Ala Met Ala Lys Phe Ser Tyr Pro Gin Glu

192

WO 01/85772 PCT/GB01/02003

• • • ·· • · • ·« * • • • • · • • ·· e 9 9999 9 9 • ♦ • • • • 9 9 ·· ··«· • · ··· • · 9 9

165 170 175 Asp Ile Phe Ile Arg Ser Tyr Gin Pro Val Val Gin Gly His Ile Gly 180 185 190 Gin Ile Lys Lys Ala Val Gin Met Leu Ala Ser Ala Lys Arg Pro Val 195 200 205 Val Tyr Phe Gly Gly Gly Val Val Leu Gly Asn Ala Ser Glu Glu Leu 210 215 220 Thr Arg Phe Val Arg Met Thr Gly Ala Pro Cys Thr Gly Thr Leu Met 225 230 235 240 Gly Leu Gly Ala Tyr Pro Ser Gly Asp Arg Gin Phe Leu Gly Met Leu 245 250 255 Gly Met His Gly Thr Tyr Glu Ala Asn Leu Ala Met Gin Asn Ala Asp 260 265 270 Val Val Leu Ala Val Gly Ala Arg Phe Asp Asp Arg Val Val Ser Val 275 280 285 Pro Ser Lys Phe Phe Glu Lys Ala Lys Lys Val Ile His Ile Asp Val 290 295 300 Asp Pro Ser Ser Ile Ala Lys Arg Val Lys Ala Asp Ile Pro Ile Val 305 310 315 320 Gly Asp val Lys Asn Val Leu ser Glu Met Val Ala Leu Trp Gin Lys 325 330 335 Gin Glu Ser Val Pro Ser Glu Asp Ala Leu Gly Lys Trp Trp Lys Thr 340 345 350 Ile Glu Glu Trp Arg Ser Arg Asp Cys Leu Trp Phe Ašp Asn Gly Ser 355 360 365 Glu Ile Ile Lys Pro Gin Tyr Val Ile Gin Lys Leu Ala Glu Ile Thr 370 Ί i375 380 Gly Asn Ser Ala Ile Ile Thr Ser Asp Val Gly Gin His Gin Met Phe 385 390 395 400 Ala Ala Gin Tyr Tyr Pro Phe Glu Arg Pro Arg Gin Trp Leu Asn Ser 405 410 415 Gly Gly Leu Gly Thr Met Gly Val Gly Leu Pro Tyr Ala Ile Gly Ala

193

·· • · 4 • S ·«*· ·· 1 · β • ·· · • β · · • β · • · .· ·· ··» WO 01/85772 PCT/GB01/02003 420 . 425 430 Lys Leu Ala Ala Pro Asp Gin Asp Val Phe Cys Ile Thr Gly Asp Gly 435 440 445 Ser Ile Gin Met Asn Ile Gin Glu Leu Ser Thr Cys Phe Gin Tyr Arg 450 455 460 Ile Pro Val Asn Val Ile Thr Leu Asn Asn Gly Tyr Leu Gly Met Val 465 470 475 480 Arg Gin Trp Gin Glu Ile Tyr Tyr Gly Gly Arg Glu Ser Glu Thr Tyr 485 490 495 Phe Asp Ser Leu Pro Asp Phe Val Lys Leu Ala Glu Ala Tyr Gly His 500 . 505 510 Ile Gly Ile Arg Val Asp Lys Lys Ser Asp Val Glu Gly Ala Leu Leu 515 520 525 Glu Ala Leu Asn Gin Lys Asp Arg Leu Val Phe Ile Asp Phe Leu Thr 530 535 540 Asp Gin Lys Gin Asn Val Met Pro Met Val Gly Asn Gly Lys Gly Leu 545 550 555 560 Asp Glu Met Val Leu Pro Pro His Met Arg Ala Asp Gly Lys Ala 565 570 575

<210> 97 <211> 570 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(570) <400> 97

atg Met 1 gac aat cac Asp Asn His gcc Ala 5 gaa gca cac tgg caa aac ggc tgg ctt caa agc Leu Gin Ser 15 48 Glu Ala His Trp Gin 10 Asn Gly Trp ata cgc cat acc ccg tcg ccc aat ttc agc ccg agg gaa acg ggg gaa 96 Ile Arg His Thr Pro Ser Pro Asn Phe Ser Pro Arg Glu Thr Gly Glu 20 25 30

194 •fl ·· ·· • · · · · · • · · · • · · · · · · · · ···· ···· ·· • · ·· ····

WO 01/85772 PCT/GB01/02003

acg gtt tcc Thr Val Ser ctg Leu atc Ile gtg Val ttg cac aac Asn att tea ctg ccg ccg ttc gaa Glu 144 Leu His 40 Ile Ser Leu Pro 45 Pro Phe 35 tac ggc acg gat gct gtg gaa aag ctg ttt gcc aac cgg ctc gac ccc 192 Tyr Gly Thr Asp Ala Val Glu Lys Leu Phe Ala Asn Arg Leu Asp Pro 50 55 60 aac gga cat ccg ttc ttc age ctg ata cac act ttg cgc gta tcc age 240 Asn Gly His Pro Phe Phe Ser Leu Ile His Thr Leu Arg Val Ser Ser 65 70 75 80 cat ttc tta atc aaa cgc gac ggc aaa acg gtg cag ttc gta tea tgc 288 His Phe Leu Ile Lys Arg Asp Gly Lys Thr Val Gin Phe Val ser Cys 85 90 95 ggc gat atg gcg tac cac gcg ggc gta tcc tcg ttt cgc gga cgg gaa 336 Gly Asp Met Ala Tyr His Ala Gly Val Ser Ser Phe Arg Gly Arg Glu 100 105 110 aaa tgc aac gca ttt tcc atc ggc atc gaa ttg gaa ggc tgc gat ttc 384 Lys Cys Asn Ala Phe Ser Ile Gly Ile Glu Leu Glu Gly Cys Asp Phe 115 120 125 gaa ccc ttt acc gaa gcg caa tac cgt tcg ctc gaa aca ttg ttg gaa 432 Glu Pro Phe Thr Glu Ala Gin Tyr Arg Ser Leu Glu Thr Leu Leu Glu 130 135 140 gca ctc tgc cgc cgc tac ccc gtt acc gca gta acc gga cat cag gac 480 Ala Leu Cys Arg Arg Tyr Pro Val Thr Ala Val Thr Gly His Gin Asp 145 150 155 160 atc gcg ccc ggc cgc aaa acc gac ccc ggc cac ttt ttc gac tgg cgg 528 Ile Ala Pro Gly Arg Lys Thr Asp Pro Gly His Phe Phe Asp Trp Arg 165 170 175 cgg ata cgg gag aaa ggg ttt ccc gta gac aga aat gcc gtc 570 Arg Ile Arg Glu Lys Gly Phe Pro Val Asp Arg Asn Ala Val 180 185 190

<210> 98 <211> 190 <212> PRT <213> Neisseria meningitidis <400> 98

195

WO 01/85772 PCT/GBO1/02003

·· ·· • · · • · · · · · • · · · • · · · • · · 9 · • * · • · · · ···· ···· • e · · · » · · ·

Met Asp Asn His Ala Glu Ala His Trp Gin Asn Gly Trp Leu Gin Ser 1 5 10 15 Xle Arg His Thr Pro Ser Pro Asn Phe Ser Pro Arg Glu Thr Gly Glu 20 25 30 Thr Val Ser Leu Ile Val Leu His Asn Ile Ser Leu Pro Pro Phe Glu 35 • 40 45 Tyr Gly Thr Asp Ala Val Glu Lys Leu Phe Ala Asn Arg Leu Asp Pro 50 55 60. Asn Gly His Pro Phe Phe Ser Leu Ile His Thr Leu Arg Val Ser Ser 65 70 75 80 His Phe Leu Ile Lys Arg Asp GÍy Lys Thr Val Gin Phe Val Ser Cys 85 90 95 Gly Asp Met Ala Tyr His Ala Gly Val Ser Ser Phe Arg Gly Arg Glu 100 105 110 Lys Cys Asn Ala Phe Ser Ile Gly Ile Glu Leu Glu Gly Cys Asp Phe 115 120 125 Glu Pro Phe Thr Glu Ala Gin Tyr Arg Ser Leu Glu Thr Leu Leu Glu 130 135 140 Ala Leu Cys Arg Arg Tyr Pro Val Thr Ala Val Thr Gly His Gin Asp 145 150 155 160 Ile Ala Pro Gly Arg Lys Thr Asp Pro Gly His Phe Phe Asp Trp Arg 165 170 175 Arg Ile Arg Glu Lys Gly Phe Pro Val Asp Arg Asn Ala Val 180 185 190

<210> 99 <211> 1515 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1515) <400> 99

196 • · · ·

WO 01/85772 PCT/GB01/02003 • · ··· ···· ··· ···· ·· ··· ·· ··

atg ttg tat ttc aga tac ggt ttt ttg gtt gtt tgg tgt gcg gca ggt Gly 48 Met Leu Tyr Phe Arg Tyr Gly Phe Leu Val 10 Val Trp Cys Ala Ala 15 1 5 gtt tet gcc gcc tat ggg gcg gat gcg ccc gcg att ttg gat gac aag 96 Val Ser Ala Ala Tyr Gly Ala Asp Ala Pro Ala Ile Leu Asp Asp Lys 20 25 30 gca ttg ttg cag gtg cag cgg tcg gtg tcg gat aag tgg gcg gaa tcg 144 Ala Leu Leu Gin Val Gin Arg Ser Val Ser Asp Lys Trp Ala Glu Ser 35 40 45 gat tgg aaa gtt gac aat gat gcc ccg cgc gtg gtt gac ggg gat ttt 192 Asp Trp Lys Val Asp Asn Asp Ala Pro Arg Val Val Asp Gly Asp Phe 50 55 60 ttg ttg gcg cat ccg aaa atg ttg gaa cat agt ttg cgc gac gtg ctc 240 Leu Leu Ala His Pro Lys Met Leu Glu His Ser Leu Arg Asp Val Leu 65 70 75 80 aac ggc aat cag gcg gat ttg atc get tcg ttg gcg gat ttg tat gcc 288 Asn Gly Asn Gin Ala Asp Leu Ile Ala Ser Leu Ala Asp Leu Tyr Ala 85 90 95 aag ctg ccg gat tat gac gcg gtt ttg tac ggc agg gcg cgg get ttg 336 Lys Leu Pro Asp Tyr Asp Ala Val Leu Tyr Gly Arg Ala Arg Ala Leu 100 105 110 ctg gcg aaa ttg gcg gga· agg ccg gcg gag gcg gtg gcg cgg tat cgg 384 Leu Ala Lys Leu Ala Gly Arg Pro Ala Glu Ala Val Ala Arg Tyr Arg 115 120 125 gaa ctg cac ggg gaa aat gcg gca gac gag cgg att ttg ctg gat ttg 432 Glu Leu His Gly Glu Asn Ala Ala Asp Glu Arg Ile Leu Leu Asp Leu 130 135 140 gcg gcg gcg gag ttt gac gat ttc cgg ctg aag tcg gca gaa agg cat 480 Ala Ala Ala Glu Phe Asp Asp Phe Arg Leu Lys Ser Ala Glu Arg His 145 150 155 160 ttt gcc gag gcg gaa aaa ttg gat ttg ccg gcg ccg gtt ttg gaa aat 528 Phe Ala Glu Ala Glu Lys Leu Asp Leu Pro Ala Pro Val Leu Glu Asn 165 170 175 gtg ggg cgt ttt cgg aaa aaa gcg gag ggg ctg acg ggc tgg cgt ttt 576 Val Gly Arg Phe Arg Lys Lys Ala Glu Gly Leu Thr Gly Trp Arg Phe 180 185 190

197 • · · · .·’'·! i

WO 01/85772

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tcg ggc ggc atc agt ccg gcg gtc aat aga aat gcc aat aat gcc gcg 624 Ser Gly Gly Ile Ser Pro Ala Val Asn Arg Asn Ala Asn Asn Ala Ala 195 200 205 ccg cag tat tgc cgg caa aac gga ggc cgg cag ata tgc agt gtc agc 672 Pro Gin Tyr Cys Arg Gin Asn Gly Gly Arg Gin Ile Cys Ser Val Ser 210 215 220 cgg gcg gag cgg gcg gca ggc ttg aat tat gaa atc gag gcg gaa aaa 720 Arg Ala Glu Arg Ala Ala Gly Leu Asn Tyr Glu Ile Glu Ala Glu Lys 225 230 235 240 ctg acg gcg ttg gca gat aat cat tat ttg ttg ttc cgt tcc aat atc 768 Leu Thr Ala Leu Ala Asp Asn His Tyr Leu Leu Phe Arg Ser Asn Ile 245 250 255 ggc ggc acg agc tat tat ttc agt aaa aaa tea gct tat gac gac ggg 816 Gly Gly Thr Ser Tyr Tyr Phe Ser Lys Lys Ser Ala Tyr Asp Asp Gly 260 265 270 ttc ggc aga gcg tat ttg ggt tgg cag tat aaa aat gca cgg cag acg 864 Phe Gly Arg Ala Tyr Leu Gly Trp Gin Tyr Lys Asn Ala Arg Gin Thr 275 280 285 gcg ggg att ttg ccg ttt tat cag gtg cag ttg tcg ggc agc gac ggc 912 Ala Gly Ile Leu Pro Phe Tyr Gin Val Gin Leu Ser Gly Ser Asp Gly 290 295 300 ttt gat gcg aaa aca aaa cgg gta aac aac cgc cgc ctg ccg ccg tat 960 Phe Asp Ala Lys Thr Lys Arg Val Asn Asn Arg Arg Leu Pro Pro Tyr 305 310 315 320 atg ctg gcg cac gga gtc ggc gtg cag ttg tcc cat act tac cgc cca 1008 Met Leu Ala His Gly Val Gly Val Gin Leu Ser His Thr Tyr Arg Pro 325 330 335 aac ccg gga tgg caa ttt tcg gtc gcg ctg gaa cat tac cgc caa cgc 1056 Asn Pro Gly Trp Gin Phe Ser Val Ala Leu Glu His Tyr Arg Gin Arg 340 345 350 tac cgc gaa cag gat agg gcg gaa tac aat aac ggt cgg cag gac ggg 1104 Tyr Arg Glu Gin Asp Arg Ala Glu Tyr Asn Asn Gly Arg Gin Asp Gly 355 360 365 ttt tat gtt tcg tcg gca aaa cgt ttg ggc gaa tcg gca act gtg ttc 1152 Phe Tyr Val Ser Ser Ala Lys Arg Leu Gly Glu Ser Ala Thr Val Phe

370

375

380

198 ·· · · ···· • ·

WO 01/85772 PCT/GB01/02003

ggc ggc tgg cag ttt gtg cgg ttt gtg ccg aaa cgc gaa acg gtg ggc 1200 Gly 385 Gly Trp Gin Phe Val 390 Arg Phe Val Pro Lys 395 Arg Glu Thr Val Gly 400 ggc gcg gtc aat aat gcc gcc tac cgg cgc aac ggt gtt tat gcc ggc 1248 Gly Ala Val Asn Asn Ala Ala Tyr Arg Arg Asn Gly Val Tyr Ala Gly 405 410 415 tgg gcg cag gag tgg cgg cag ttg ggc ggt ttg aac agt cgg gtt tcc 1296 Trp Ala Gin Glu Trp Arg Gin Leu Gly Gly Leu Asn Ser Arg Val Ser 420 425 430 gcg tet tat gcc cgc cgc aac tat aag ggc gtt gcg get ttc tcg aca 1344 Ala Ser Tyr Ala Arg Arg Asn Tyr Lys Gly Val Ala Ala Phe Ser Thr 435 440 445 gag gcg caa cgc aac cgc gaa tgg aat gtc tcg ctg get ttg agc cac 1392 Glu Ala Gin Arg Asn Arg Glu Trp Asn Val Ser Leu Ala Leu Ser His 450 455 460 gac aag ttg tcg tac aaa ggt atc gtg ccc gcg ttg aat tat cgt ttc 1440 Asp Lys Leu Ser Tyr Lys Gly Ile Val Pro Ala Leu Asn Tyr Arg Phe 465 470 475 480 ggc agg acg gaa agt aat gtg ccg tat gcg aaa cgc cgc aac agc gag 1488 Gly Arg Thr Glu Ser Asn Val Pro Tyr Ala Lys Arg Arg Asn Ser Glu 485 490 495 gtg ttt gtg tcg gcg gat tgg cgg ttt 1515 Val Phe Val Ser Ala Asp Trp Arg Phe 500 505

<210> 100 <211> 505 <212> PRT <213> Neisseria meningitidis <400> 100

Met Leu Tyr Phe Arg Tyr; Gly Phe Leu Val Val Trp Cys Ala Ala Gly 1 5 10 15 Val Ser Ala Ala Tyr Gly Ala Asp Ala Pro Ala Ile Leu Asp Asp Lys 20 25 30 Ala Leu Leu Gin Val Gin Arg Ser Val Ser Asp Lys Trp Ala Glu Ser

35 40 45

199

. · · «'· • · <· ·· • · · · β e • · • ft · • • · · • · • '9 4 • • · < · • '· · • ' · ···· ··«· • ·'· ♦ · • ·

WO 01/85772 PCT/GBO1/02003 Asp Trp Lys Val Asp Asn Asp Ala Pro Arg Val Val Asp Gly Asp Phe 50 55 60 Leu Leu Ala His Pro Lys Met Leu Glu His Ser Leu Arg Asp 65 70 75 Val Leu 80 Asn Gly Asn Gin Ala Asp Leu Ile Ala Ser Leu Ala Asp Leu 85 90 Tyr Ala 95 Lys Leu Pro Asp Tyr Asp Ala Val Leu Tyr Gly Arg Ala Arg 100 105 110 Ala Leu Leu Ala Lys Leu Ala Gly AJjg Pro Ala Glu Ala Val Ala Arg 115 120 125 Tyr Arg Glu Leu His Gly Glu Asn Ala Ala Asp Glu Arg Ile Leu Leu 130 135 140 Asp Leu Ala Ala Ala Glu Phe Asp Asp Phe Arg Leu Lys Ser Ala Glu 145 150 155 Arg His 160 Phe Ala Glu Ala Glu Lys Leu Asp Leu Pro Ala Pro Val Leu 165 170 Glu Asn 175 Val Gly Arg Phe Arg Lys Lys Ala Glu Gly Leu Thr Gly Trp 180 185 190 Arg Phe Ser Gly Gly Ile Ser Pro Ala Val Asn Arg Asn Ala Asn Asn 195 200 205 Ala Ala Pro Gin Tyr Cys Arg Gin Asn Gly Gly Arg Gin Ile Cys Ser 210 215 220 Val Ser Arg Ala Glu Arg Ala Ala Gly Leu Asn Tyr Glu Ile Glu Ala 225 230 235 Glu Lys 240 Leu Thr Ala Leu Ala Asp Asn His Tyr Leu Leu Phe Arg Ser 245 250 Asn Ile 255 Gly Gly Thr Ser Tyr Tyr 'Phe Ser Lys Lys Ser Ala Tyr Asp 260 265 270 Asp Gly Phe Gly Arg Ala Tyr Leu Gly Trp Gin Tyr Lys Asn Ala Arg 275 280 285 Gin Thr Ala Gly Ile Leu Pro Phe Tyr Gin Val Gin Leu Ser Gly Ser 290 295 300 Asp Gly

200 ·· ···» • » ·

WO 01/85772 PCT/GBO1/02003 Phe Asp Ala Lys Thr Lys Arg Val Asn Asn Arg Arg Leu Pro 305 310 315 Pro Tyr 320 Met Leu Ala His Gly Val Gly Val Gin Leu Ser His Thr Tyr 325 330 Arg Pro 335 Asn Pro Gly Trp Gin Phe Ser Val Ala Leu Glu His Tyr Arg 340 345 350 Gin Arg Tyr Arg Glu Gin Asp Arg Ala Glu Tyr Asn Asn Gly Arg Gin 355 360 365 Asp Gly Phe Tyr Val Ser Ser Ala Lys Arg Leu Gly Glu Ser Ala Thr 370 375 380 Val Phe Gly Gly Trp Gin Phe Val Arg Phe Val Pro Lys Arg Glu Thr 385 390 395 Val Gly 400 Gly Ala Val Asn Asn Ala Ala Tyr Arg Arg Asn Gly Val Tyr 405 410 Ala Gly 415 Trp Ala Gin Glu Trp Arg Gin Leu Gly Gly Leu Asn Ser Arg 420 425 430 Val Ser Ala Ser Tyr Ala Arg Arg Asn Tyr Lys Gly Val Ala Ala Phe 435 440 445 Ser Thr Glu Ala Gin Arg Asn Arg Glu Trp Asn Val Ser Leu Ala Leu 450 455 460 Ser His Asp Lys Leu Ser Tyr Lys Gly Ile Val Pro Ala Leu Asn Tyr Arg Phe 465 470 475 480 Gly Arg Thr Glu Ser Asn Val Pro Tyr Ala Lys Arg Arg Asn 485 490 Val Phe Val Ser Ala Asp Trp Arg Phe Ser Glu 495

500 505 <210> 101 <211> 1476 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS

201

WO 01/85772 PCT/GBO1/02003 <222> (1)..(1476) <400> 101

atg aaa tac aaa gac ctg cgc gac ttc atc gcc atg ctc gag cag cag 48 Met 1 Lys Tyr Lys Asp 5 Leu Arg Asp Phe Ile 10 Ala Met Leu Glu Gin 15 Gin ggc aaa ctc aaa cgc atc gcg cac ccc gtt tcc ccg cat ttg gaa atg 96 Gly Lys Leu Lys Arg Ile Ala His Pro Val Ser Pro His Leu Glu Met 20 25 30 acc gaa atc gcc gac cgc gtg ctg cgc gcc gaa ggg ccg gcg ttg ttg 144 Thr Glu Ile Ala Asp Arg Val Leu Arg Ala Glu Gly Pro Ala Leu Leu 35 40 45 ttt gaa cac cca gtt aag ccc gac ggt acg cgc tat gat tat ccc gtg 192 Phe Glu His Pro Val Lys Pro Asp Gly Thr Arg Tyr Asp Tyr Pro Val 50 55 60 ttg gca aac ctg ttc ggc acg ccc gaa cgt gtg gcg atg ggc atg ggc 240 Leu Ala Asn Leu Phe Gly Thr Pro Glu Arg Val Ala Met Gly Met Gly 65 70 75 80 gcg gac agc gtg tcc aag ctg cgc gaa atc ggg cag acg ctg gcg tat 288 Ala Asp Ser Val Ser Lys Leu Arg Glu Ile Gly Gin Thr Leu Ala Tyr 85 90 95 ttg aaa gaa ccc gaa ccg ccc aaa ggc att aaa gac gcg ttt tcc aaa 336 Leu Lys Glu Pro Glu Pro Pro Lys Gly Ile Lys Asp Ala Phe Ser Lys 100 105 110 ctg ccg ctc ttg aaa gac att tgg agc atg gcg ccg aac gtg gtg aaa 384 Leu Pro Leu Leu Lys Asp Ile Trp Ser Met Ala Pro Asn val Val Lys 115 120 125 aat gcg ccg tgt cag gaa atc gta tgg gaa ggc gaa gac gtt gat ttg 432 Asn Ala Pro Cys Gin Glu Ile Val Trp Glu Gly Glu Asp Val Asp Leu 130 135 140 tat caa ctt ccg att cag' 'cat tgc tgg ccg gaa gac gtt gcg Ccg ctg 480 Tyr Gin Leu Pro Ile Gin His Cys Trp Pro Glu Asp Val Ala Pro Leu 145 150 155 160 gta acg tgg ggc ttg acc gtc acg cgc ggg ccg cac aaa aaa cgc caa 528 Val Thr Trp Gly Leu Thr Val Thr Arg Gly Pro His Lys Lys Arg Gin 165 170 175 aat ctc ggc att tac cgc caa caa tta atc ggc ata aac aag ctg att 576

202

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Asn Leu Gly Ile Tyr Arg Gin Gin Leu Ile Gly Ile Asn Lys Leu Ile 180 185 190 atg cgc tgg ctg tcg cat cgc ggc ggc gcg ctg gat tat cag gaa ttc 624 Met Arg Trp Leu Ser His Arg Gly Gly Ala Leu Asp Tyr Gin Glu Phe 195 200 205 cgc aaa ctc aat ccc gat acg ccg tat ccc gtc gcc gtc gtg ctc ggc 672 Arg Lys Leu Asn Pro Asp Thr Pro Tyr Pro Val Ala Val Val Leu Gly 210 215 220 tgc gac cct gcc acc att ttg ggc gcg gta acg ccc gtt ccc gat acc 720 Cys Asp Pro Ala Thr Ile Leu Gly Ala Val Thr Pro Val Pro Asp Thr 225 230 235 240 ttg agc gaa tac cag ttt gcc gga ctg ctg cgc ggt tcg cgg acg gaa 768 Leu Ser Glu Tyr Gin Phe Ala Gly Leu Leu Arg Gly Ser Arg Thr Glu 245 250 255 ctg gtg aaa tgt atc ggc aac gat ttg caa gtg cct gcc cgc gca gaa 816 Leu Val Lys Cys Ile Gly Asn Asp Leu Gin Val Pro Ala Arg Ala Glu 260 265 270 atc gtg ttg gaa ggc gtc atc cat cca aac gaa acc gcg ttg gaa ggt 864 Ile Val' Leu Glu Gly Val Ile His Pro Asn Glu Thr Ala Leu Glu Gly 275 280 285 cca tac ggc gac cac acg ggc tat tac aac gag cag gac cat ttc ccc 912 Pro Tyr Gly Asp His Thr Gly Tyr Tyr Asn Glu Gin Asp His Phe Pro 290 295 300 gtg ttt acg gtc gaa cgc atc acc atg cgc gaa aac ccg att tac cac 960 Val Phe Thr Val Glu Arg Ile Thr Met Arg Glu Asn Pro Ile Tyr His 305 310 315 320 tet acc tac aca ggc aaa ccg ccc gat gaa ccc gcc gtt ttg ggc gtg 1008 Ser Thr Tyr Thr Gly Lys Pro Pro Asp Glu Pro Ala Val Leu Gly Val 325 330 335 gcg ttg aac gaa gtg ttc \gta ccg ctt ttg caa aag cag ttc ccc gaa 1056 Ala Leu Asn Glu Val Phe Val Pro Leu Leu Gin Lys Gin Phe Pro Glu 340 345 350 atc acc gat ttt tac ctg ccg ccc gaa ggc tgc tcc tac cgc atg gcg 1104 Ile Thr Asp Phe Tyr Leu Pro Pro Glu Gly Cys Ser Tyr Arg Met Ala 355 360 365 gtg gtg agc atg aaa aaa cag tac gcc gga cac gcc aag cgc gtg atg 1152

203

WO 01/85772 PCT/GB01/02003

«4 ·» • *· 4444 • • • • · • 4 • 4 • • • • 4 • 4 4 • • • • 4 4 • 4 4 ·*· • • · · • • 4 • ·· 44 • 4

Val Val 370 Ser Met Lys Lys Gin Tyr Ala Gly His Ala Lys Arg Val Met 375 380 atg ggc tgc tgg tcg ttc ctg cgc cag ttt atg tac acc aaa ttc atc 1200 Met Gly Cys Trp Ser Phe Leu Arg Gin Phe Met Tyr Thr Lys Phe Ile 385 390 395 400 att gtg gtg gat gac gat gtg gat gtg cgc gac tgg aaa gaa gtc atc 1248 Ile Val Val Asp Asp Asp Val Asp Val Arg Asp Trp Lys Glu Val Ile 405 410 415 tgg gcg gta acc acg cgc atg gac ccc gtg cgc gat acc gtt ttg atg 1296 Trp Ala Val Thr Thr Arg Met Asp Pro Val Arg Asp Thr Val Leu Met 420 425 430 gaa aac acg ccc atc gac tac ctc gac ttc gcc age ccc gtc age gga 1344 Glu Asn Thr Pro Ile Asp Tyr Leu Asp Phe Ala Ser Pro Val Ser Gly 435 440 445 ctt ggc ggc aaa atg ggt ttg gat gcg acc aac aag tgg ccg ggc gaa 1392 Leu Gly Gly Lys Met Gly Leu Asp Ala Thr Asn Lys Trp Pro Gly Glu 450 455 460 acc gac cgc gaa tgg gga cgg gtg att aaa aaa gac cct gcg gtt acg 1440 Thr Asp Arg Glu Trp Gly Arg Val Ile Lys Lys Asp Pro Ala Val Thr 465 470 475 480 gct aag att gat gag att tgg gag gaa ttg ggg ttg 1476 Ala Lys Ile Asp Glu Ile Trp Glu Glu Leu Gly Leu 485 490

<210> 102 <211> 492 <212> PRT <213> Neisseria meningitidis <400> 102

Met 1 Lys Tyr Lys Asp 5 Leu Arg Asp Phe Ile 10 Ala Met Leu Glu Gin 15 Gin Gly Lys Leu Lys 20 Arg Ile Ala His Pro 25 Val Ser Pro His Leu 30 Glu Met Thr Glu Ile 35 Ala Asp Arg Val Leu 40 Arg Ala Glu Gly Pro 45 Ala Leu Leu Phe Glu His Pro Val Lys Pro Asp Gly Thr Arg Tyr Asp Tyr Pro Val

204

WO 01/85772 PCT/GB01/02003

« · e> ·> 94 e « • · • 4 9 4 94 9 4 4 9 4 999 9 • β • 0 0 4 • • 4 4 4 • 4 • · ··· • • · V 4 4 9 49 4 44 • ·

50 55 60 Leu Ala Asn Leu Phe Gly Thr Pro Glu Arg Val Ala Met Gly Met Gly 65 70 75 80 Ala Asp Ser Val Ser Lys Leu Arg Glu Ile Gly Gin Thr Leu Ala Tyr 85 90 95 Leu Lys Glu Pro Glu Pro Pro Lys Gly Ile Lys Asp Ala Phe Ser Lys 100 105 110 Leu Pro Leu Leu Lys Asp Ile Trp Ser Met Ala Pro Asn Val val Lys 115 120 125 Asn Ala Pro Cys Gin Glu Ile Val Trp Glu Gly Glu Asp Val Asp Leu 130 135 140 Tyr Gin Leu Pro Ile Gin His Cys Trp Pro Glu Asp Val Ala Pro Leu 145 150 155 160 Val Thr Trp Gly Leu Thr Val Thr Arg Gly Pro His Lys Lys Arg Gin 165 170 175 Asn Leu Gly Ile Tyr Arg Gin Gin Leu Ile Gly Ile Asn Lys Leu Ile 180 185 190 Met Arg Trp Leu Ser His Arg Gly Gly Ala Leu Asp Tyr Gin Glu Phe 195 200 205 Arg Lys Leu Asn Pro Asp Thr Pro Tyr Pro Val Ala Val Val Leu Gly 210 215 220 Cys Asp Pro Ala Thr Ile Leu Gly Ala Val Thr Pro Val Pro Asp Thr 225 230 235 240 Leu Ser Glu Tyr Gin Phe Ala Gly Leu Leu Arg Gly Ser Arg Thr Glu 245 250 255 Leu Val Lys Cys Ile Gly Asn Asp Leu Gin Val Pro Ala Arg Ala Glu 260 265 270 Ile Val Leu Glu Gly Val Ile His Pro Asn Glu Thr Ala Leu Glu Gly 275 280 285 Pro Tyr Gly Asp His Thr Gly Tyr Tyr Asn Glu Gin Asp His Phe Pro 290 295 300 Val Phe Thr Val Glu Arg Ile Thr Met Arg GlU Asn Pro Ile Tyr His

205

V /· .. „ * r :

· · • 4 9494 4444 ·« > ·« • · ·« «·

WO 01/85772 PCT/GB01/02003

305 310 315 320

Ser Thr Tyr Thr Gly Lys Pro Pro Asp Glu Pro Ala Val Leu Gly Val 325 330 335 Ala Leu Asn Glu Val Phe Val Pro Leu Leu Gin Lys Gin Phe Pro Glu 340 345 350 Ile Thr Asp Phe Tyr Leu Pro Pro Glu Gly Cys Ser Tyr Arg Met Ala 355 360 365 Val Val Ser Met Lys Lys Gin Tyr Ala Gly His Ala Lys Arg Val Met 370 375 380 Met Gly Cys Trp Ser Phe Leu Arg Gin Phe Met Tyr Thr Lys Phe Ile 385 390 395 400 Ile Val Val Asp Asp Asp Val Asp Val Arg Asp Trp Lys Glu Val Ile 405 410 415 Trp Ala Val Thr Thr Arg Met Asp Pro Val Arg Asp Thr Val Leu Met 420 425 430 Glu Asn Thr Pro Ile Asp Tyr Leu Asp Phe Ala Ser Pro Val Ser Gly 435 440 445 Leu Gly Gly Lys Met Gly Leu Asp Ala Thr Asn Lys Trp Pro Gly Glu 450 455 460 Thr Asp Arg Glu Trp Gly Arg Val Ile Lys Lys Asp Pro Ala Val Thr 465 470 475 480 Ala Lys Ile Asp Glu Ile Trp Glu Glu Leu Gly Leu

485 490 <210> 103 <211> 1089 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1089) <400> 103 atg agt ttg aaa tgc ggc atc gtc ggt ttg ccc aac gtc ggc aaa tcc 48

206

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PCT/GBO1/02003

Met Ser Leu Lys Cys Gly Xle Val Gly Leu Pro Asn Val Gly Lys Ser 1 5 10 15 acc ctt ttt aac gcg ctg acc caa tcg ggt atc gaa gcg gca aac tat 96 Thr Leu Phe Asn Ala Leu Thr Gin Ser Gly Ile Glu Ala Ala Asn Tyr 20 25 30 cct ttc tgt acc atc gaa ccc aac gtc ggc atc gtc gaa gtc ccc gat 144 Pro Phe Cys Thr Ile Glu Pro Asn Val Gly Ile Val Glu Val Pro. Asp 35 40 45 ccg cgt atg gcc gaa ttg gca aaa atc gtc aat ccg caa aaa atg cag 192 Pro Arg Met Ala Glu Leu Ala Lys Ile Val Asn Pro Gin Lys Met Gin 50 55 60 cct gcc atc gtc gaa ttt gtc gát att gcc ggt ttg gtt gca ggc gcg 240 Pro Ala Ile Val Glu Phe Val Asp Ile Ala Gly Leu Val Ala Gly Ala 65 70 75 80 age aaa ggc gag ggc ttg ggc aac cag ttc ctt gcc aac atc cgc gaa 288 Ser Lys Gly Glu Gly Leu Gly Asn Gin Phe Leu Ala Asn Ile Arg Glu 85 90 95 acc gat gcg att gtg aat gtc gtg cgc tgc ttt gac gac gac aac atc 336 Thr Asp Ala Xle val Asn Val Val Arg Cys Phe Asp Asp Asp Asn Ile 100 105 110 gtc cac gtt gca ggc cgc gtc gat ccg att gcc gac att gaa acc atc 384 Val His Val Ala Gly Arg Val Asp Pro Ile Ala Asp Ile Glu Thr Ile 115 120 125 ggc aca gag ttg gca ctt gcc gac ctg gca agt gtc gaa aaa gcc atc 432 Gly Thr Glu Leu Ala Leu Ala Asp Leu Ala Ser Val Glu Lys Ala Ile 130 135 140 gtc cgc gaa gaa aaa cgc gcc cgc tca ggc gac aaa gac gcg caa aag 480 Val Arg Glu Glu Lys Arg Ala Arg Ser Gly Asp Lys Asp Ala Gin Lys 145 150 155 160 ctg gtc gat ttg tgc aaa ;aaa ctg ctg ccg cat ctg gac gaa ggc aaa 528 Leu Val Asp Leu Cys Lys Lys Leu Leu Pro His Leu Asp Glu Gly Lys 165 170 175 ccc gtg cgt tcc ttc ggt ttg gac gcg gaa gaa cgc gcg atg ctc aaa 576 Pro Val Arg Ser Phe Gly Leu Asp Ala Glu Glu Arg Ala Met Leu Lys 180 185 190 ccg ctg ttc ctg ctg acc gcc aaa ccg gcg atg tat gtg ggc aac gtc 624

207

WO 01/85772 PCT/GBO1/02003 Pro Leu Phe Leu Leu Thr Ala Lys Pro Ala Met Tyr Val Gly Asn Val 195 200 205 gcc gaa gac ggt ttt gaa aac aat ccg cac ctc gac cgc ctg aaa gaa 672 Ala Glu Asp Gly Phe Glu Asn Asn Pro His Leu Asp Arg Leu Lys Glu 210 215 220 ttg gcg gca aaa gaa aac gcc ccc gtc gtc gcc gtt tgc gcc gcg atg 720 Leu Ala Ala Lys Glu Asn Ala Pro Val Val Ala Val Cys Ala Ala Met 225 230 235 240 gag age gaa att gcc gaa ttg gaa gac gac gaa aaa gcc gag ttc ctc 768 Glu Ser Glu Ile Ala Glu Leu Glu Asp Asp Glu Lys Ala Glu Phe Leu 245 250 255 gcc gaa atg ggc ttg gaa gaa ccg ggc ctg aac cgc ctg att cgt gcc 816 Ala Glu Met Gly Leu Glu Glu Pro Gly Leu Asn Arg Leu Ile Arg Ala

260 265 270

ggt tac gac ctc ttg ggg Gly ctg caa acc tat ttc acc gcc ggt gtg aaa 864 Gly Tyr Asp Leu Leu Leu Gin Thr Tyr Phe Thr Ala Gly Val Lys 275 280 285 gaa gtc cgc gcg tgg acg ata cac aaa ggc gat acc gcc ccg caa gcc 912 Glu Val Arg Ala Trp Thr Ile His Lys Gly Asp Thr Ala Pro Gin Ala 290 295 300 gcc ggc gtg att cac acc gac ttt gag cgc ggc ttc atc cgc gcc caa 960 Ala Gly Val Ile His Thr Asp Phe Glu Arg Gly Phe Ile Arg Ala Gin 305 310 315 320 gtc att tet tac gac gac ttt gtc tcg ctc ggc ggc gaa gcc aaa gcc 1008 Val Ile Ser Tyr Asp Asp Phe Val Ser Leu Gly Gly Glu Ala Lys Ala 325 330 335 aaa gaa gcc ggc aaa atg cgt gtg gaa ggt aag gaa tac gtc gtg cag 1056 Lys Glu Ala Gly Lys Met Arg Val Glu Gly Lys Glu Tyr Val Val Gin 340 345 350 gac ggc gat gta atg cac ttt ttg ttt aac gtg 1089 Asp Gly Asp Val Met His Phe Leu Phe Asn Val 355 360

<210> 104 <211> 363 <212> PRT <213> Neisseria meningitidis

208 ··

WO 01/85772

PCT/GBO1/02003 <400> 104

Met 1 Ser Leu Lys Cys 5 Gly Ile Val Gly Leu 10 Pro Asn Val Gly Lys 15 Ser Thr Leu Phe Asn 20 Ala Leu Thr Gin Ser 25 Gly Ile Glu Ala Ala 30 Asn Tyr Pro Phe Cys 35 Thr Ile Glu Pro Asn 40 Val Gly Ile Val Glu 45 Val Pro Asp Pro Arg 50 Met Ala Glu Leu Ala 55 Lys Ile Val Asn Pro 60 Gin Lys Met Gin Pro 65 Ala Ile Val Glu Phe 70 Val Asp Ile Ala Gly 75 Leu Val Ala Gly Ala 80 Ser Lys Gly Glu Gly 85 Leu Gly Asn Gin Phe 90 Leu Ala Asn Ile Arg 95 Glu Thr Asp Ala Xle 100 Val Asn Val Val Arg 105 Cys Phe Asp Asp Asp 110 Asn Ile Val His Val 115 Ala Gly Arg Val Asp 120 Pro Ile Ala Asp Ile 125 Glu Thr Ile Gly Thr 130 Glu Leu Ala Leu Ala 135 Asp Leu Ala Ser Val 140 Glu Lys Ala Ile Val 145 Arg Glu Glu Lys Arg 150 Ala Arg Ser Gly Asp 155 Lys Asp Ala Gin Lys 160 Leu Val Asp Leu Cys 165 Lys Lys Leu Leu Pro 170 His Leu Asp Glu Gly 175 Lys Pro Val Arg Ser 180 Phe Gly Leu Asp Ala 185 Glu Glu Arg Ala Met 190 Leu Lys Pro Leu Phe 195 Leu Leu Thr Ala Lys 200 Pro Ala Met Tyr Val 205 Gly Asn Val Ala Glu 210 Asp Gly Phe Glu Asn 215 Asn Pro His Leu Asp 220 Arg Leu Lys Glu Leu 225 Ala Ala Lys Glu Asn 230 Ala Pro Val Val Ala 235 Val Cys Ala Ala Met 240 GlU Ser Glu Ile Ala Glu Leu Glu Asp Asp Glu Lys Ala Glu Phe Leu

209

WO 01/85772

PCT/GB01/02003 • · ···· · ·

245 250 255 Ala Glu Met Gly Leu Glu Glu Pro Gly Leu Asn Arg Leu Ile Arg Ala 260 265 270 Gly Tyr Asp Leu Leu Gly Leu Gin Thr Tyr Phe Thr Ala Gly Val Lys 275 280 285 Glu Val Arg Ala Trp Thr Ile His Lys Gly Asp Thr Ala Pro Gin Ala 290 295 300 Ala Gly Val Ile His Thr Asp Phe Glu Arg Gly Phe Ile Arg Ala Gin 305 310 315 320 Val Ile Ser Tyr Asp Asp Phe Val Ser Leu Gly Gly Glu Ala Lys Ala 325 330 335 Lys Glu Ala Gly Lys Met Arg Val Glu Gly Lys Glu Tyr Val Val Gin 340 345 350 Asp Gly Asp Val Met His Phe Leu Phe Asn Val 355 360 • <210> 105 <211> 1177 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1).. (1176) <400> 105 atg gca aaa atg atg aaa tgg gcg gct gtt gcg gcg gtc gcg gcg gca 48 Met Ala Lys Met Met Lys Trp Ala Ala Val Ala Ala Val Ala Ala Ala 1 5 10 15 gcg gtt tgg ggc gga tgg tet tat ctg aag ccc gag ccg cag gct gct 96 Ala Val Trp Gly Gly Trp Ser Tyr Leu Lys Pro Glu Pro Gin Ala Ala 20 25 30 tat att acg gaa acg gtc agg cgc ggc gac atc agc cgg acg gtt tet 144 Tyr Ile Thr Glu Thr Val Arg Arg Gly Asp Ile Ser Arg Thr Val Ser 35 40 45 gca aca ggg gag att tcg ccg tcc aac ctg gta tcg gtc ggc gcg cag 192

210 ·· ·«>··

WO 01/85772

PCT/GB01/02003

Ala Thr Gly Glu Ile Ser Pro Ser Asn Leu Val Ser Val Gly Ala Gin 50 55 60 gca tcg ggg cag att aag aaa ctt tat gtc aaa ctc ggg caa cag gtt 240 Ala Ser Gly Gin Ile Lys Lys Leu Tyr Val Lys Leu Gly Gin Gin Val 65 70 75 80 aaa aag ggc gat ttg att gcg gaa atc aat tcg acc tcg cag acc aat 288 Lys Lys Gly Asp Leu Ile Ala Glu Ile Asn Ser Thr Ser Gin Thr Asn 85 90 95 acg ctc aat acg gaa aaa tcc aaa ttg gaa acg tat cag gcg aag ctg 336 Thr Leu Asn Thr Glu Lys Ser Lys Leu Glu Thr Tyr Gin Ala Lys Leu 100 105 110 gtg tcg gca cag att gca ttg ggc agc gcg gag aag aaa tat aag cgt 384 Val Ser Ala Gin Ile Ala Leu Gly Ser Ala Glu Lys Lys Tyr Lys Arg 115 120 -- 125 cag gcg gcg ttg tgg aag gat gat gcg acc gct aaa gaa gat ttg gaa 432 Gin Ala Ala Leu Trp Lys Asp Asp Ala Thr Ala Lys Glu Asp Leu Glu 130 135 140 agc gca cag gat gcg ctt gcc gcc gcc aaa gcc aat gtt gcc gag ctg 480 Ser Ala Gin Asp Ala Leu Ala Ala Ala Lys Ala Asn Val Ala Glu Leu 145 150 155 160 aag gct cta atc aga cag agc aaa att tcc atc aat acc gcc gag tcg 528 Lys Ala Leu Ile Arg Gin Ser Lys Ile Ser Ile Asn Thr Ala Glu Ser 165 170 175 gaa ttg ggc tac acg cgc att acc gca acg atg gac ggc acg gtg gtg 576 Glu Leu Gly Tyr Thr Arg Ile Thr Ala Thr Met Asp Gly Thr Val Val 180 185 190 gcg att ctc gtg gaa gag ggg cag act gtg aac gcg gcg cag tet acg 624 Ala Ile Leu Val Glu Glu Gly Gin Thr Val Asn Ala Ala Gin Ser Thr 195 200 205 ccg acg att gtc caa ttg 'gcg aat ctg gat atg atg ttg aac aaa atg 672 Pro Thr Ile Val Gin Leu Ala Asn Leu Asp Met Met Leu Asn Lys Met 210 215 220 cag att gcc gag ggc gat att acc aag gtg aag gcg ggg cag gat att 720 Gin Ile Ala Glu Gly Asp Ile Thr Lys Val Lys Ala Gly Gin Asp Ile 225 230 235 240 tcg ttt acg att ttg tcc gaa ccg gat acg ccg att aag gcg aag ctc 768

211 • i β β ·· ··

WO 01/85772 PCT/GB01/02003 Ser Phe Thr Ile Leu Ser Glu Pro Asp Thr Pro Ile Lys Ala Lys Leu 245 250 255 gac agc gtc gac ccc ggg ctg acc acg atg tcg tcg ggc ggc tac aac 816 Asp Ser Val Asp Pro Gly Leu Thr Thr Met Ser Ser Gly Gly Tyr Asn 260 265 270 agc agt acg gat acg gct tcc aat gcg gtc tac tat tat gcc cgt tcg 864 Ser Ser Thr Asp Thr Ala Ser Asn Ala Val Tyr Tyr Tyr Ala Arg Ser 275 280 285 ttt gtg ccg aat ccg gac ggc aaa ctc gcc acg ggg atg acg acg cag 912 Phe Val Pro Asn Pro Asp Gly Lys Leu Ala Thr Gly Met Thr Thr Gin 290 295 300 aat acg gtt gaa atc gac ggt gtg aaa aat gtg ctg att att ccg tcg 960 Asn Thr Val Glu Ile Asp Gly Val Lys Asn val Leu Ile Ile Pro Ser 305 310 315 320 ctg acc gtg aaa aat cgc ggc ggc agg gcg ttt gtg cgc gtg ttg ggt 1008 Leu Thr Val Lys Asn Arg Gly Gly Arg Ala Phe Val Arg Val Leu Gly 325 330 335 gca gac ggc aag gcg gcg gaa cgc gaa atc cgg acc ggt atg aga gac 1056 Ala Asp Gly Lys Ala Ala Glu Arg Glu Ile Arg Thr Gly Met Arg Asp

340 345 350

agt atg aat acc gaa gta aaa agc ggg ttg aaa gag ggg gac aaa gtg 1104 Ser Met Asn 355 Thr Glu Val Lys Ser Gly Leu 360 Lys Glu Gly 365 Asp Lys Val gtc atc tcc gaa ata acc gcc gcc gag cag cag gaa agc ggc gaa cgc 1152 Val Ile Ser Glu Ile Thr Ala Ala Glu Gin Gin. Glu Ser Gly Glu Arg 370 375 380

gcc cta ggc ggc ccg ccg cgc ega t 1177 Ala Leu Gly Gly Pro Pro Arg Arg 385 390

<210> 106 <211> 392 <212> PRT <213> Neisseria meningitidis <400> 106

Met Ala Lys Met Met Lys Trp Ala Ala Val Ala Ala Val Ala Ala Ala 15 10 15

212 fcfc • fc fcfcfc fcfc fcfc fc fcfc · fc · fc fcfc » · fc fc fcfcfc fc' fc · • · fcfcfc ··· • fcfcfc fcfcfcfc fcfc fcfcfc fcfc fcfc

WO 01/85772 PCT/GB01/02003 Ala Val Trp Gly 20 Gly Trp Ser Tyr Leu 25 Lys Pro Glu Pro Gin . 30 Ala Ala Tyr Ile Thr 35 GlU Thr Val Arg Arg 40 Gly Asp Ile Ser Arg 45 Thr Val Ser Ala Thr 50 Gly Glu Ile Ser Pro 55 Ser Asn Leu Val Ser 60 Val Gly Ala Gin Ala 65 Ser Gly Gin Ile Lys 70 Lys Leu Tyr Val Lys 75 Leu Gly Gin Gin Val 80 Lys Lys Gly Asp Leu 85 Ile Ala Glu Ile Asn 90 Ser Thr Ser Gin Thr 95 Asn Thr Leu Asn Thr 100 Glu Lys Ser Lys Leu 105 Glu Thr Tyr Gin Ala 110 Lys Leu Val Ser Ala 115 Gin Ile Ala Leu Gly 120 Ser Ala Glu Lys Lys 125 Tyr Lys Arg Gin Ala 130 Ala Leu Trp Lys Asp 135 Asp Ala Thr Ala Lys 140 Glu Asp Leu Glu Ser 145 Ala Gin Asp Ala Leu 150 Ala Ala Ala Lys Ala 155 Asn Val Ala Glu Leu 160 Lys Ala Leu Ile Arg 165 Gin Ser Lys Ile Ser 170 Ile Asn Thr Ala Glu 175 Ser Glu Leu Gly Tyr 180 Thr Arg Ile Thr Ala 185 Thr Met Asp Gly Thr 190 Val Val Ala Ile Leu 195 Val Glu Glu Gly Gin 200 Thr Val Asn Ala Ala 205 Gin Ser Thr Pro Thr 210 Ile Val Gin Leu Ala 215 Asn Leu Asp Met Met 220 Leu Asn Lys Met Gin 225 Ile Ala Glu Gly Asp 230 Ile Thr Lys Val Lys 235 Ala Gly Gin Asp Ile 240 Ser Phe Thr Ile Leu 245 Ser Glu Pro Asp Thr 250 Pro Ile Lys Ala Lys 255 Leu Asp Ser Val Asp 260 Pro Gly Leu Thr Thr 265 Met Ser Ser Gly Gly 270 Tyr Asn

213 •4 ·· ·· · ·· 4444

4 9 4 4 9 4 44 9 4 9

4 9 4 9 4 4 ·4

4 4 9 4 4 9 4 4 9 9

4 9 4 4 » 4 9 9 4

WO 01/85772

PCT/GBO1/02003

Ser Ser Thr 275 Asp Thr Ala Ser Asn 280 Ala Val Tyr Tyr Tyr 285 Ala Arg Ser Phe Val 290 Pro Asn Pro Asp Gly 295 Lys Leu Ala Thr Gly 300 Met Thr Thr Gin Asn 305 Thr Val Glu Ile Asp 310 Gly Val Lys Asn Val 315 Leu Ile Ile Pro Ser 320 Leu Thr Val Lys Asn 325 Arg Gly Gly Arg Ala 330 Phe Val Arg Val Leu 335 Gly Ala Asp Gly Lys 340 Ala Ala Glu Arg Glu 345 ne Arg Thr Gly Met 350 Arg Asp Ser Met Asn 355 Thr Glu Val Lys Ser 360 Gly Leu Lys Glu Gly Asp 365 Lys Val Val Ile 370 Ser Glu Ile Thr Ala 375 Ala Glu Gin GÍn Glu 380 Ser Gly Glu Arg Ala 385 Leu Gly Gly Pro Pro 390 Arg Arg

<210> 107 <211> 1185 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1185)

<400> 107 atg aca gag gac gaa cgt ttc gcg Met 1 Thr Glu Asp Glu 5 Arg Phe Ala tat atc ggc gcg gaa agt ttc ctg Tyr Ile Gly Ala 20 Glu Ser Phe Leu gcg caa aat gcc ctg tcc gca ccg Ala Gin Asn 35 Ala Leu Ser Ala Pro 40

tgg ctg caa ttg gcg ttt acg ccc Pro 48 Trp Leu Gin Leu Ala Phe Thr .15 10 ctg ctg atg cgc cgt ttc ggc age 96 Leu Leu Met Arg Arg Phe Gly Ser 25 30 gcg gaa cag gtg gcg gca ctg ata 144 Ala Glu Gin Val Ala Ala Leu Ile

214

99 ···· ♦ · ·· ·· « • · · 9 9 9 99 ♦ 9 · · · • · · · ♦ · • « 9 9 9 •9999999 99 9 99

WO 01/85772

PCT/GBO1/02003

cgg cac aaa cag gcg ctt gag get tgg cgc aat gcg gaa aaa cgc get 192 Arg His 50 Lys Gin Ala Leu Glu 55 Ala Trp Arg Asn Ala 60 Glu Lys Arg Ala ctg gcg cgg cag gcg gca gaa gcg gcg ttg gaa tgg gaa atg cgg gac 240 Leu Ala Arg Gin Ala Ala Glu Ala Ala Leu Glu Trp Glu Met Arg Asp 65 70 75 80 gga tgc cgc ctg atg ctg ctt cag gat gaa gat ttc ccc gaa atg ctg 288 Gly Cys Arg Leu Met Leu Leu Gin Asp Glu Asp Phe Pro Glu Met Leu 85 90 95 acg cag ggg ctg acc gcg ccg ccg gtt ttg ttt ttg cgc ggc aac gtg 336 Thr Gin Gly Leu Thr Ala Pro Pro Val Leu Phe Leu Arg Gly Asn Val 100 105 110 cgg ctg ctg cac aag cct tcc gcc gcc atc gtc ggc age cgc cat gcc 384 Arg Leu Leu His Lys Pro Ser Ala Ala Ile Val Gly Ser Arg His Ala 115 120 125 acg ccg cag gcg atg cgg att gcc aaa gat ttc ggc aag tcg ttg ggc 432 Thr Pro Gin Ala Met Arg Ile Ala Lys Asp Phe Gly Lys Ser Leu Gly 130 135 140 ggg caa aat atc ccc gtt gtg tcg ggt atg get tcc ggt atc gat act 480 Gly Gin Asn Ile Pro Val Val Ser Gly Met Ala Ser Gly Ile Asp Thr 145 150 155 160 gcc gcc cat cag ggc gca ctg gag gca gaa ggc ggc acc atc gcc gtg 528 Ala Ala His Gin Gly Ala Leu Glu Ala Glu Gly Gly Thr Ile Ala Val 165 170 175 tgg ggg acg ggt ata gac cgc att tac ccg ccg tcc aat aaa aac ctt 576 Trp Gly Thr Gly Ile Asp Arg Ile Tyr Pro Pro Ser Asn Lys Asn Leu 180 185 190 gcc tat gaa atc gcc gaa aaa gga ttg att gtc age gag ttt ccc atc 624 Ala Tyr Glu Ile Ala Glu Lys Gly Leu Ile Val Ser Glu Phe Pro Ile 195 200 205 ggt acg cgc ccg tat gcc ggc aat ttt ccg cgc cgc aac cgc ctg att 672 Gly Thr Arg Pro Tyr Ala Gly Asn Phe Pro Arg Arg Asn Arg Leu Ile 210 215 220 gcc gcc ctg tcg caa gta acg ctg gtg gtt gaa gcc gcc ttg gaa tcc 720 Ala Ala Leu Ser Gin Val Thr Leu Val Val Glu Ala Ala Leu Glu Ser 225 230 235 240

215 ·· ·· • · « e • · • · • · ···· ····

99 9999

9 9 9

9 9 9

9 9 9

9 9 9 9 ·· 99

WO 01/85772 PCT/GB01/02003 • β β· • · ·

9 9 9

9 9 9

99 999

ggt Gly tcg ctg att acc gcc ggc ctg Gly Leu gcg Ala gca gaa atg ggg cgc gaa gtg 768 Ser Leu Ile Thr 245 Ala Ala 250 Glu Met Gly Arg Glu Val 255 atg gcg gta ccc ggt tcg ata gac aat ccg cac agc aaa ggc tgc cac 816 Met Ala Val Pro Gly Ser Ile Asp Asn Pro His Ser Lys Gly Cys His 260 265 270 aaa ctg att aaa gac ggc gca aaa ttg gtg gaa tgc ctg gac gac atc 864 Lys Leu Ile Lys Asp Gly Ala Lys Leu Val Glu Cys Leu Asp Asp Ile 275 280 285 ctg aac gaa tgc ccg ggg cta ttg caa aat acg ggt gct tea tea tat 912 Leu Asn Glu Cys Pro Gly Leu Leu Gin Asn Thr Gly Ala Ser Ser Tyr 290 295 300 tet ata aat aag gat acg ccc gac acg gga cgc cgc gcc gtt cag acg 960 Ser Ile Asn Lys Asp Thr Pro Asp Thr Gly Arg Arg Ala Val Gin Thr 305 310 315 320 gca tac gcc ccg ccg cct gcc gca aaa atg ccg tet gaa gcg gcg gca 1008 Ala Tyr Ala Pro Pro Pro Ala Ala Lys Met Pro Ser Glu Ala Ala Ala 325 330 335 ggc ggc aca gcc gtc ggc ggc ata ctg gat aaa atg ggt ttc gac ccc 1056 Gly Gly Thr Ala Val Gly Gly Ile Leu Asp Lys Met Gly Phe Asp Pro 340 345 350 atc cat ccc gac gtg ctt gcc gga cag ttg gct atg cct gcc gca gat 1104 Ile His Pro Asp val Leu Ala Gly Gin Leu Ala Met Pro Ala Ala Asp 355 360 365 ttg tat gcc gca ctg ttg gaa ttg gaa ttg gac ggc agc gtt gcc gca 1152 Leu Tyr Ala Ala Leu Leu Glu Leu Glu Leu Asp Gly Ser Val Ala Ala 370 375 380 atg ccc agc ggc aga tac cag cgt atc ega act 1185 Met Pro Ser Gly Arg Tyr. . Gin Arg Ile Arg Thr

385 390 ' 395 <210> 108 <211> 395 <212> PRT <213> Neisseria meningitidis <400> 108

216 ο β e e e β·· · · · • · ·· · · ? « • ··*· ···· · • · · · · · · · ·

WO 01/85772 PCT/GB01/02003

Met 1 Thr Glu Asp Glu 5 Arg Phe Ala Trp Leu 10 Gin Leu . Ala Phe Thr 15 Pro Tyr Xle Gly Ala 20 Glu Ser Phe Leu Leu 25 Leu Met Arg Arg Phe 30 Gly Ser Ala Gin Asn 35 Ala Leu Ser Ala Pro 40 Ala Glu Gin Val Ala 45 Ala Leu Ile Arg His 50 Lys Gin Ala Leu Glu 55 Ala Trp Arg Asn Ala 60 Glu Lys Arg Ala Leu 65 Ala Arg Gin Ala Ala 70 Glu Ala Ala Leu Glu 75 Trp Glu Met Arg Asp 80 Gly Cys Arg Leu Met 85 Leu Leu Gin Asp Glu 90 Asp Phe Pro Glu Met 95 Leu Thr Gin Gly Leu 100 Thr Ala Pro Pro Val 105 Leu Phe Leu Arg Gly 110 Asn Val Arg Leu Leu 115 His Lys Pro Ser Ala 120 Ala Ile Val Gly Ser 125 Arg His Ala Thr Pro 130 Gin Ala Met Arg Ile 135 Ala Lys Asp Phe Gly 140 Lys Ser Leu Gly Gly 145 Gin Asn Xle Pro Val 150 Val Ser Gly Met Ala 155 Ser Gly Ile Asp Thr 160 Ala Ala His Gin Gly 165 Ala Leu Glu Ala Glu 170 Gly Gly Thr Ile Ala 175 Val Trp Gly Thr Gly 180 Ile Asp Arg Ile Tyr 185 Pro Pro Ser Asn Lys 190 Asn Leu Ala Tyr Glu 195 Xle Ala Glu Lys Gly 200 Leu Ile Val Ser Glu 205 Phe Pro Ile Gly Thr 210 Arg Pro Tyr Ala '-Gly 215 Asn Phe Pro Arg Arg 220 Asn Arg Leu Ile Ala 225 Ala Leu Ser Gin Val 230 Thr Leu Val Val Glu 235 Ala Ala Leu Glu Ser 240 Gly Ser Leu Ile Thr 245 Ala Gly Leu . Ala Ala 250 Glu Met Gly Arg Glu 255 Val

217 • · β β β 9 9 4 4 4 4 9

4 · 9 · 4 · '·♦ • · β 4 · 9 9

4 4 9 9 ' 9 ··· · ······· ·· 499 44 44

WO 01/85772 PCT/GB01/02003

Met Ala Val Pro Gly Ser Ile Asp Asn Pro His Ser Lys Gly Cys His 260 265 270 Lys Leu Ile- Lys Asp Gly Ala Lys Leu Val Glu Cys Leu Asp Asp Ile 275 280 285 Leu Asn Glu Cys Pro Gly Leu Leu Gin Asn Thr Gly Ala Ser Ser Tyr 290 295 300 Ser Ile Asn Lys Asp Thr Pro Asp Thr Gly Arg Arg Ala Val Gin Thr 305 310 315 320 Ala Tyr Ala Pro Pro Pro Ala Ala Lys Met Pro Ser Glu Ala Ala Ala 325 330 335 Gly Gly Thr Ala Val Gly Gly Ile Leu Asp Lys Met Gly Phe Asp Pro 340 345 350 Ile His Pro Asp Val Leu Ala Gly Gin Leu Ala Met Pro Ala Ala Asp 355 360 365 Leu Tyr Ala Ala Leu Leu Glu Leu Glu Leu Asp Gly Ser Val Ala Ala 370 375 380 Met Pro Ser Gly Arg Tyr Gin Arg Ile Arg Thr 385 390 395

<210> 109 <211> 1257 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1257) <400> 109 atg aaa cag acc gtc ctc -aaa aat aac ctg caa aac ctg ctt gaa age 48

Met Lys Gin Thr Val Leu Lys Asn Asn Leu Gin Asn Leu Leu Glu Ser

15 10 15 gca gaa aat atc ctg ctg ctt caa ggc cct gtc ggc gat ttt ttt ctg 96

Ala Glu Asn Ile Leu Leu Leu Gin Gly Pro Val Gly Asp Phe Phe Leu

20 25 30 cgc ctt gcc gac tgg ctg act gca aac ggc aaa acc gta cat aaa ttc 144

218 'i.

• ·

• • • · · • • • · · · • • • . · • · • · • · • ·υ ·< WO 01/85772 PCT/GB01/02003 Arg Leu Ala Asp Trp Leu Thr Ala Asn Gly Lys Thr Val His Lys Phe 35 40 45 aac ttt aat gca ggc gac gac tat ttt tat ccg ccc act caa gcg cat 192 Asn Phe Asn Ala Gly Asp Asp Tyr Phe Tyr Pro Pro Thr Gin Ala His 50 55 60 acc gtt gtt ttt aac gac aac tac gat gcc ttt cct gag ttt ttg caa 240 Thr Val Val Phe Asn Asp Asn Tyr Asp Ala Phe Pro Glu Phe Leu Gin 65 70 75 80 gaa tac atc act caa cat cac atc cag gcc gtt gtc tgc ttt ggc gac 288 Glu Tyr Ile Thr Gin His His Ile Gin Ala Val Val Cys Phe Gly Asp 85 90 95 aca cgc cct tat cac gtc att gfca aaa cgc att gca aac gaa aac caa 336 Thr Arg Pro Tyr His Val Ile Ala Lys Arg Ile Ala Asn Glu Asn Gin 100 105 110 gcc agt ttc tgg gcg ttt gaa gaa ggc tat ttc cgc ccc tac tac atc 384 Ala Ser Phe Trp Ala Phe Glu Glu Gly Tyr Phe Arg Pro Tyr Tyr Ile 115 120 125 acc tta gaa aaa gac ggc gtc aac gca ttt tcc ccg ttg ccg cgc cgt 432 Thr Leu Glu Lys Asp Gly Val Asn Ala Phe Ser Pro Leu Pro Arg Arg 130 135 140 gcc gac ttt ttt ctt gaa caa ttc cct aag ctt gcc cag caa gaa tat 480 Ala Asp Phe Phe Leu Glu Gin Phe Pro Lys Leu Ala Gin Gin Glu Tyr 145 150 155 160 aaa gcg cca acg ccg gta cac ggc ggt ttt acg ccc atg gca aaa aac 528 Lys Ala Pro Thr Pro Val His Gly Gly Phe Thr Pro Met Ala Lys Asn 165 170 175 get atc cgt tac tat atc gag ttg ttc cgc aat cta cgc aaa tac ccc 576 Ala Ile Arg Tyr Tyr Ile Glu Leu Phe Arg Asn Leu Arg Lys Tyr Pro 180 185 190 gac tac atc cac cac cgc '.gca ccc aat gcc ggc cat tac ctc aaa ccg 624 Asp Tyr Ile His His Arg Ala Pro Asn Ala Gly His Tyr Leu Lys Pro 195 200 205 tgg tcg ctc tcc atc ctc aag cgt ttg aac tac tat att gaa gac atc 672 Trp Ser Leu Ser Ile Leu Lys Arg Leu Asn Tyr Tyr Ile Glu Asp Ile 210 215 220 caa atc gcc aaa cgt gtg gaa gca ggc aaa tac ggc aag ttt ttt att 720

219 ·· * * • · β· · to · · ·

Íte · ·.-·♦ • · ·

WO 01/85772

PCT/GB01/02003

Gin 225 Ile Ala Lys Arg Val 230 Glu Ala Gly gtt ccc ttg cag gta ttc aac gac agc Val Pro Leu Gin Val 245 Phe Asn Asp Ser ttt ccc agc gtc cgc agc ttc ctg ctc Phe Pro Ser Val 260 Arg Ser Phe Leu Leu 265 gaa cac gcg cct gcc gat acc aac atc Glu His Ala 275 Pro Ala Asp Thr Asn 280 Ile gac cgc ggc ttt atc gac tac tgg cgc Asp Arg 290 Gly Phe Ile Asp Tyr 295 Trp Arg gaa cac ccc gaa ctc aaa ggc cgt gtg Glu 305 His Pro Glu Leu Lys 310 Gly Arg Val ctg ccc gtt ttc ctg cgc cac ggt ctc Leu Pro Val Phe Leu 325 Arg His Gly Leu acc agc ggc ctg tcc gga ctg att cac Thr Ser Gly Leu 340 Ser Gly Leu Ile His 345 ggc cgt gcc tat tat gat att ccc ggc Gly Arg Ala 355 Tyr Tyr Asp Ile Pro 360 Gly gca gaa ttt tgg aat cat ccg aca ccg Ala Glu 370 Phe Trp Asn His Pro 375 Thr Pro gcc tac ega atg tac cac' ctc aac gtg Ala 385 Tyr Arg Met Tyr His 390 Leu Asn Val tac agt cag gtc ttt ttt cct aac aaa Tyr Ser Gin Val Phe Phe Pro Asn Lys

405

Lys Tyr 235 Gly Lys Phe Phe Ile 240 caa gtc cgt atc cat tgc gac 768 Gin 250 Val Arg Ile His Cys 255 Asp cat gtt ttg agt tea ttt gcc 816 His Val Leu Ser Ser 270 Phe Ala atc atc aag cat cat cct atg 864 Ile Ile Lys His 285 His Pro Met gac att aaa cgc ttt atc aaa 912 Asp Ile Lys 300 Arg Phe Ile Lys att tat gtc cat gat gtc ccc 960 Ile Tyr 315 Val His Asp Val Pro 320 ggc atg gtt acc atc aac agc 1008 Gly 330 Met Val Thr Ile Asn 335 Ser aat atg cca gtt aag gtt ctc 1056 Asn Met Pro Val Lys 350 Val Leu att act gac caa aat acc ttg 1104 Ile Thr Asp Gin 365 Asn Thr Leu cct gac aaa gag ctg ttc cat 1152 Pro Asp Lys 380 Glu Leu Phe His acc caa att aac ggc aac ttc 1200 Thr Gin 395 Ile Asn Gly Asn Phe 400 aac aca tcc gac tet tcc aca 1248 Asn 410 Thr Ser Asp Ser Ser 415 Thr

1257 cca aca acc

220

WO 01/85772 PCT/GB01/02003

Pro Thr Thr .· <210> 110 <211> 419 <212> PRT <213> Neisseria meningitidis <400> 110

Met 1 Lys Gin Thr Val 5 Leu Lys Asn Asn Leu 10 Gin Asn Leu Leu Glu 15 Ser Ala Glu Asn Ile 20 Leu Leu Leu Gin Gly 25 Pro Val Gly Asp Phe 30 Phe Leu Arg Leu T -¾ 35 Asp Trp Leu Thr Ala 40 Asn Gly Lys Thr Val 45 His Lys Phe Asn Phe 50 Asn Ala Gly Asp Asp 55 Tyr Phe Tyr Pro Pro 60 Thr Gin Ala His Thr 65 Val Val Phe Asn Asp 70 Asn Tyr Asp Ala Phe 75 Pro Glu Phe Leu Gin 80 Glu Tyr Ile Thr Gin 85 His His Ile Gin Ala 90 Val Val Cys Phe Gly Asp 95 Thr Arg Pro Tyr 100 His Val Ile Ala Lys 105 Arg Ile Ala Asn Glu 110 Asn Gin Ala Ser Phe 115 Trp Ala Phe Glu Glu 120 Gly Tyr Phe Arg Pro 125 Tyr Tyr Ile Thr Leu 130 Glu Lys Asp Gly Val 135 Asn Ala Phe Ser Pro 140 Leu Pro Arg Arg Ala 145 Asp Phe Phe Leu Glu 150 Gin Phe Pro Lys Leu 155 Ala Gin Gin Glu Tyr 160 Lys Ala Pro Thr Pro 165 Val -His Gly Gly Phe 170 Thr Pro Met Ala Lys 175 Asn Ala Ile Arg Tyr 180 Tyr Ile Glu Leu Phe 185 Arg Asn Leu Arg Lys 190 Tyr Pro Asp Tyr Ile His His Arg Ala Pro Asn Ala Gly His Tyr Leu Lys Pro

195 200 205

221 »·

WO 01/85772 PCT/GBOV02003

Trp Ser 210 Leu Ser Ile Leu Lys 215 Arg Leu Asn Tyr Tyr 220 Ile Glu Asp Ile Gin 225 Ile Ala Lys Arg Val 230 Glu Ala Gly Lys Tyr 235 Gly Lys Phe Phe Ile 240 Val Pro Leu Gin Val 245 Phe Asn Asp Ser Gin 250 Val Arg Ile His cys 255 Asp Phe Pro Ser Val 260 Arg Ser Phe Leu Leu 265 His Val Leu Ser Ser 270 Phe Ala Glu His Ala 275 Pro Ala Asp Thr Asn 280 Ile Ile Ile Lys His 285 His Pro Met Asp Arg 290 Gly Phe Ile Asp Tyr 295 Trp Arg Asp Ile Lys 300 Arg Phe Ile Lys Glu 305 His Pro Glu Leu Lys 310 Gly Arg Val Ile Tyr 315 Val His Asp Val Pro 320 Leu Prů Val Phe Leu 325 Arg His Gly Leu Gly 330 Met Val Thr Ile Asn 335 Ser Thr Ser Gly Leu 340 Ser Gly Leu Ile His 345 Asn Met Pro Val Lys 350 Val Leu Gly Arg Ala 355 Tyr Tyr Asp Ile Pro 360 Gly Ile Thr Asp Gin 365 Asn Thr Leu Ala Glu 370 Phe Trp Asn His Pro 375 Thr Pro Pro Asp Lys 380 Glu Leu Phe His Ala 385 Tyr Arg Met Tyr His 390 Leu Asn Val Thr Gin 395 Ile Asn Gly Asn Phe 400 Tyr Ser Gin Val Phe 405 Phe Pro Asn Lys Asn 410 Thr Ser Asp Ser Ser 415 Thr

Pro Thr Thr <210> 111 <211> 1674 <212> DNA <213> Neisseria meningitidis

222

WO 01/85772 PCT/GB01/02003 <220>

<221> CDS <222> (1)..(1674) <400> 111

atg agt ttc aaa Lys acc gat gcc gaa atc gcc caa tcc tcc acc atg cgc 48 Met Ser 1 Phe Thr 5 Asp Ala Glu Ile Ala 10 Gin Ser Ser Thr Met Arg 15 ccg att ggc gaa att gcc gcc aag ctg ggt ttg aac gtt gac aac att 96 Pro Xle Gly Glu 20 Ile Ala Ala Lys Leu 25 Gly Leu Asn val Asp Asn Ile 30 gag cct tac ggt cat tac aaa gcc aaa atc aat cct gcc gaa gcg ttc 144 Glu Pro Tyr 35 Gly His Tyr Lys Ala 40 Lys Ile Asn Pro Ala 45 Glu Ala Phe aaa ctg ccg caa aaa cag ggc agg ctg att ttg gtt acc gcc atc aac 192 Lys Leu 50 Pro Gin Lys Gin Gly Arg 55 Leu Ile Leu Val 60 Thr Ala Ile Asn ccg act ccg gcg ggc gaa ggt aaa acc acc gta acc atc ggt ttg gcg 240 Pro Thr 65 Pro Ala Gly Glu 70 Gly Lys Thr Thr Val 75 Thr Ile Gly Leu Ala 80 gac gca ttg cgc cat atc ggc aaa gac tet gtg att gct ttg cgc gag 288 Asp Ala Leu Arg His 85 Ile Gly Lys Asp Ser 90 Val Ile Ala Leu Arg Glu 95 cct tet ttg ggt ccg gtg ttc ggc gtg aaa ggc ggc gcg gca ggc ggc 336 Pro Ser Leu Gly 100 Pro Val Phe Gly Val 105 Lys Gly Gly Ala Ala Gly Gly 110 ggc tat gcc caa gtt ttg ccg atg gaa gac atc aac ctg cac ttc acc 384 Gly Tyr Ala 115 Gin Val Leu Pro Met 120 Glu Asp Ile Asn Leu 125 His Phe Thr gga gat ttt cac gcc atc ggt gcg gca aat aat ctg ctt gcc gcg atg 432 Gly Asp 130 Phe His Ala Ile -Gly Ala 135 Ala Asn Asn Leu 140 Leu Ala Ala Met ctc gac aac cat atc tac caa ggc aac gag ttg aac atc gac ccc aaa 480 Leu Asp 145 Asn His Ile Tyr 150 Gin Gly Asn Glu Leu 155 Asn Ile Asp Pro Lys 160 cgc gtg ctg tgg cgg cgc gtg gtc gat atg aac gac cgc cag ttg cgc 528 Arg Val Leu Trp Arg Arg Val Val Asp Met Asn Asp Arg Gin Leu Arg

223

·· «0 ·· • ·> · · · 9 9 9 9 • · 9 9 • 9 · · • • 9 9 9.9 *'· · · 9 „•-•l 0» • · • • · 99 9 • · • ·

WO 01/85772 PCT/GB01/02003

165 170 175 aac atc atc gac ggc atg ggc aag cct gtt gac ggc gtg atg cgt cct 576 Asn Ile Ile Asp 180 Gly Met Gly Lys Pro 185 Val Asp Gly Val Met 190 Arg Pro gac ggt ttc gat att acc gtt get tcc gaa gtg atg gcg gta ttc tgt 624 Asp Gly Phe 195 Asp Ile Thr Val Ala 200 Ser Glu Val Met Ala 205 Val Phe Cys ctt gcc aaa gac atc age gat ttg aaa gag cgt ttg ggc aac atc ctt 672 Leu Ala 210 Lys Asp Ile Ser Asp 215 Leu Lys Glu Arg Leu 220 Gly Asn Ile Leu gtc gcc tac gcc aaa gac ggc age ccc gtt tac gcc aaa gat ttg aaa 720 Val 225 Ala Tyr Ala Lys Asp 230 Gly Sér Pro Val Tyr 235 Ala Lys Asp Leu Lys 240 gcg aat ggc gcg atg gcg gca ttg ctt aaa gat gcg att aag ccc aac 768 Ala Asn Gly Ala Met 245 Ala Ala Leu Leu Lys 250 Asp Ala Ile Lys Pro 255 Asn ttg gtg caa acc atc gaa ggc acg ccc gcc ttc gta cac ggc ggc ccg 816 Leu Val Gin Thr 260 Ile Glu Gly Thr Pro 265 Ala Phe Val His Gly 270 Gly Pro ttc gcc aac atc gcc cac ggc tgc aac tcc gta acc gca acc cgt ctg 864 Phe Ala Asn 275 Ile Ala His Gly Cys 280 Asn Ser Val Thr Ala 285 Thr Arg Leu gcg aaa cac ctt gcc gat tac gcc gta acc gaa gca ggc ttc ggc gcg 912 Ala Lys 290 His Leu Ala Asp Tyr 295 Ala Val Thr Glu Ala 300 Gly Phe Gly Ala gac ttg ggc gcg gaa aaa ttc tgc gac atc aaa tgc cgc ctt gcc ggt 960 Asp 305 Leu Gly Ala Glu Lys 310 Phe Cys Asp Ile Lys 315 Cys Arg Leu Ala Gly 320 ttg aaa cct gat gcg get. gtt gtc gtg gcg act gtc cgc gcg ttg aaa 1008 Leu Lys Pro Asp Ala 325 Ala Val Val Val Ala 330 Thr Val Arg Ala Leu 335 Lys tat aac ggc ggc gtg gaa cgc gcc aac ctc ggc gaa gaa aat tta gac 1056 Tyr Asn Gly Gly 340 Val Glu Arg Ala Asn 345 Leu Gly Glu Glu Asn 350 Leu Asp get ttg gaa aaa ggt ttg ccc aac ctg ctg aaa cac att tcc aac ctg 1104 Ala Leu Glu Lys Gly Leu Pro Asn Leu Leu Lys His Ile Ser Asn Leu

224

PCT/GBO1/02003

WO 01/85772

355 360 aaa aac gta ttc gga ctg ccc gtc gtc gtt gcg ctc Lys Asn Val Phe Gly Leu Pro Val Val Val Ala Leu 370 375 380 tcc gac tcc gat gcc gag ttg gcg atg att gaa aaa

Ser Asp Ser Asp Ala Glu Leu Ala Met Ile Glu Lys

385 390 395 cac ggc gtt gaa gtt tcc ctg acc gaa gtg tgg ggc

His Gly Val Glu Val Ser Leu Thr Glu Val Trp Gly Lys Gly Gly Ala

405 410 415

365 aac cgc ttc gtg 1152 Asn Arg Phe val gcc tgt gcc gaa 1200 Ala Cys Ala Glu 400 aaa ggt ggt gcg 1248

ggc ggc gcg gat ttg gcg cgc aaa gtc gtc aac gcc JkJLa att gaa agt caa 1296 Gly Gly Ala Asp 420 Leu Ala Arg Lýs Val 425 val Ile Glu 430 Ser Gin acc aat aac ttc ggt ttc gcc tac gat gtc gag ttg ggc atc aaa gac 1344 Thr Asn Asn Phe Gly Phe Ala Tyr Asp Val Glu Leu Gly Ile Lys Asp 435 440 445 aaa atc cgt gcg att gcc caa aaa gtg tac ggc gcg gaa gat gtt gat 1392 Lys Ile Arg Ala Ile Ala Gin Lys val Tyr Gly Ala Glu Asp Val Asp 450 455 460 ttc age gcg gaa gcg tet gcc gaa atc gct tca ctg gaa aaa ctg ggc 1440 Phe Ser Ala Glu Ala Ser Ala Glu Ile Ala Ser Leu Glu Lys Leu Gly 465 470 475 480 ttg gac aaa atg ccg atc tgc atg gcg aaa acc caa tac tet ttg age 1488 Leu Asp Lys Met Pro Ile Cys Met Ala Lys Thr Gin Tyr Ser Leu Ser 485 490 495 gac aac gcc aaa ctg ttg ggc tgc ccc gaa gac ttc cgc atc gcc gtg 1536 Asp Asn Ala Lys Leu Leu Gly Cys Pro Glu Asp Phe Arg Ile Ala Val 500 505 510 cgc ggc atc acc gtt tcc gca ggc gca ggt ttc atc gtc gcc ctg tgc 1584 Arg Gly Ile Thr Val Ser 'Ala Gly Ala Gly Phe Ile Val Ala Leu Cys 515 520 525 ggc aac atg atg aaa atg ccc ggc ctg ccc aaa gtt ccg gct gcc gag 1632 Gly Asn Met Met Lys Met Pro Gly Leu Pro Lys Val Pro Ala Ala’ Glu 530 535 540 aaa atc gat gtg gac gca gaa ggc gtg att cac ggc ttg ttc 1674 Lys Ile Asp Val Asp Ala Glu Gly Val Ile His Gly Leu Phe

225 • ·· · • ···· ··· • · * ·._· *«_,*·-· · · • · · · · * »· · ···· ···· ·· «·· ·· ··

WO 01/85772 PCT/GB01/02003

545 550 555 <210> 112 <211> 558 <212> PRT <213> Neisseria meningitidis <400> 112

Met 1 Ser Phe Lys Thr 5 Asp Ala Glu Ile Ala 10 Gin Ser Ser Thr Met 15 Arg Pro Ile Gly Glu 20 Ile Ala Ala Lys Leu 25 Gly Leu Asn Val Asp 30 Asn Ile Glu Pro Tyr 35 Gly His Tyr Lys Ala 40 Lys Ile Asn Pro Ala 45 Glu Ala Phe Lys Leu 50 Pro Gin Lys Gin Gly 55 Arg Leu Ile Leu Val 60 Thr Ala Ile Asn Pro 65 Thr Pro Ala Gly Glu 70 Gly Lys Thr Thr Val 75 Thr Ile Gly Leu Ala 80 Asp Ala Leu Arg His 85 Ile Gly Lys Asp Ser 90 Val Ile Ala Leu Arg 95 Glu Pro Ser Leu Gly 100 Pro Val Phe Gly Val 105 Lys Gly Gly Ala Ala 110 Gly Gly Gly Tyr Ala 115 Gin Val Leu Pro Met 120 Glu Asp Ile Asn Leu 125 His Phe Thr Gly Asp 130 Phe His Ala Ile Gly 135 Ala Ala Asn Asn Leu 140 Leu Ala Ala Met Leu 145 Asp Asn His Ile Tyr 150 Gin Gly Asn Glu Leu 155 Asn Ile Asp Pro Lys 160 Arg Val Leu Trp Arg 165 Arg Val Val Asp Met 170 Asn Asp Arg Gin Leu 175 Arg Asn Ile Ile Asp 180 Gly Met Gly Lys Pro 185 Val Asp Gly Val Met 190 Arg Pro Asp Gly Phe Asp Ile Thr Val Ala Ser Glu Val Met Ala Val Phe Cys

195 200 205

226 • 9 ·· ·9 · ·· ···· • 9 · 9 · · ·9 · 9 9 • 9999 9··

9 9· · 9 »99· '9 • 9 *· 99 999 9 WO 01/85772 PCT/GBO1/02003 Leu Ala Lys Asp Ile Ser Asp Leu 210 215 Lys Glu Arg Leu Gly 220 Asn Ile Leu Val 225 Ala Tyr Ala Lys Asp Gly Ser 230 Pro Val Tyr Ala Lys 235 Asp Leu Lys 240 Ala Asn Gly Ala Met Ala Ala Leu 245 Leu Lys Asp Ala Ile 250 Lys Pro 255 Asn Leu Val Gin Thr Ile Glu Gly Thr 260 Pro Ala Phe Val His 265 Gly 270 Gly Pro Phe Ala Asn Ile Ala His Gly Cys 275 280 Asn Ser Val Thr Ala 285 Thr Arg Leu Ala Lys His Leu Ala Asp Tyr Ala 290 295 Val Thr Glu Ala Gly 300 Phe Gly Ala Asp 305 Leu Gly Ala Glu Lys Phe Cys 310 Asp Ile Lys Cys Arg 315 Leu Ala Gly 320 Leu Lys Pro Asp Ala Ala Val Val 325 Val Ala Thr Val Arg 330 Ala Leu 335 Lys Tyr Asn Gly Gly Val Glu Arg Ala 340 Asn Leu Gly Glu Glu 345 Asn 350 Leu Asp Ala Leu Glu Lys Gly Leu Pro Asn 355 360 Leu Leu Lys His Ile 365 Ser Asn Leu Lys Asn Val Phe Gly Leu Pro Val 370 375 Val Val Ala Leu Asn 380 Arg Phe Val Ser 385 Asp Ser Asp Ala Glu Leu Ala 390 Met Ile Glu Lys Ala 395 Cys Ala Glu 400 His Gly Val Glu Val Ser Leu Thr 405 Glu Val Trp Gly Lys 410 Gly Gly 415 Ala Gly Gly Ala Asp Leu Ala Arg Lys 420 Val Val Asn Ala Ile 425 Glu 430 Ser Gin Thr Asn Asn Phe Gly Phe Ala Tyr 435 440 Asp Val Glu Leu Gly 445 Ile Lys Asp Lys Ile Arg Ala Ile Ala Gin Lys 450 455 Val Tyr Gly Ala Glu 460 Asp Val Asp

227 • a

WO 01/85772 PCT/GB01/02003

Phe Ser Ala Glu Ala Ser Ala Glu Ile Ala Ser Leu Glu Lys Leu Gly 465 470 475 480 Leu Asp Lys Met Pro Ile Cys Met Ala Lys Thr Gin Tyr Ser Leu Ser 485 490 495 Asp Asn Ala Lys Leu Leu Gly Cys Pro Glu Asp Phe Arg Ile Ala Val 500 505 510 Arg Gly Ile Thr Val Ser Ala Gly Ala Gly Phe Ile Val Ala Leu cys 515 520 525 Gly Asn Met Met Lys Met Pro Gly Leu Pro Lys Val Pro Ala Ala Glu 530 535 540 Lys Ile Asp Val Asp Ala Glu Gly Val Ile His Gly Leu Phe 545 550 555

<210> 113 <211> 475 <212> DNA <213> Neisseria meningitidis' <220>

<221> CDS <222> (1)..(474) <400> 113 atg gaa cac cta ttt gag gaa tgg ttg ccc gac ctg ccc gcc gac gtt 48

Met Glu His Leu Phe Glu Glu Trp Leu Pro Asp Leu Pro Ala Asp Val

15 10 15 tca gac ggc atc ggc ctg ccg atg agc cgg tta ttg aaa gcc cgg tcg 96

Ser Asp Gly Xle Gly Leu Pro Met Ser Arg Leu Leu Lys Ala Arg Ser

20 25 30 ctg act gcc gca ttg cgc gcc ttg ccg cat ctg ttt tcg gta gaa ctg 144

Leu Thr Ala Ala Leu Arg Ala Leu Pro His Leu Phe Ser Val Glu Leu

35 40 45 ctg aaa ctg ggc gaa ttg gaa acg gaa tgc gga ggg cgg ctg gtg cgc 192

Leu Lys Leu Gly Glu Leu Glu Thr Glu Cys Gly Gly Arg Leu Val Arg

50 55 60 gaa gtt ttg ttg aag ctg gac ggt atc cct gtt gtc gcg gca agg agc 240

Glu Val Leu Leu Lys Leu Asp Gly Ile Pro Val Val Ala Ala Arg Ser

228 ·· ···· • 9 ·

9 9 9 · _L

WO 01/85772 PCT/GB01/02003

65 70 75 80

gaa Glu tgc cgt atc ggt Gly 85 tcg gcg ttt tgg caa aac att ttg gac tgc ggc 288 Cys Arg Ile Ser Ala Phe Trp Gin Asn 90 Ile Leu Asp Cys 95 Gly acg cgt cct ttg ggc gaa cgt ctg ttt caa gcc gat ttg gaa ggg gcg 336 Thr Arg Pro Leu Gly Glu Arg Leu Phe Gin Ala Asp Leu Glu Gly Ala 100 105 110 cgt tcg gcg ttt gag ttt gcc gtt ttc ggc gaa gga tgc gga cgg tac 384 Arg Ser Ala Phe Glu Phe Ala Val Phe Gly Glu Gly Cys Gly Arg Tyr 115 120 125 ttt gcc gct cgg cgt tcg cgg ttt tcc cat cac ggc gag gaa atg ctg 432 Phe Ala Ala Arg Arg Ser Arg Phe Ser His His Gly Glu Glu Met Leu 130 135 140 ctg acc gag tat ttt ctg ccc gaa ctg aaa cgc ttt atc gga t 475 Leu Thr Glu Tyr Phe Leu Pro Glu Leu Lys Arg Phe Ile Gly

145 150 155 <210> 114 <211> 158 <212> PRT <213> Neisseria meningitidis <400> 114

Met 1 Glu His Leu Phe 5 Glu Glu Trp Leu Pro 10 Asp Leu Pro Ala Asp 15 Val Ser Asp Gly Ile 20 Gly Leu Pro Met Ser 25 Arg Leu Leu Lys Ala 30 Arg Ser Leu Thr Ala 35 Ala Leu Arg Ala Leu 40 Pro His Leu Phe Ser 45 Val Glu Leu Leu Lys 50 Leu Gly Glu Leu Glu - 55 Thr Glu cys Gly Gly Arg 60 Leu Val Arg Glu 65 Val Leu Leu Lys Leu 70 Asp Gly Ile Pro Val 75 Val Ala Ala Arg Ser 80 Glu Cys Arg Ile Gly 85 Ser Ala Phe Trp Gin 90 Asn Ile Leu Asp Cys 95 Gly Thr Arg Pro Leu Gly Glu Arg Leu Phe Gin Ala Asp Leu Glu Gly Ala

229

V «

WO 01/85772

100 Arg Ser Ala 115 Phe Glu Phe Phe Ala 130 Ala Arg Arg Ser Leu 145 Thr Glu Tyr Phe Leu 150

105

Ala Val Phe Gly Glu Gly 120

Arg Phe Ser His His Gly 135 140

Pro Glu Leu Lys Arg Phe 155

PCT/GBO1/02003

110

Cys Gly Arg Tyr 125

Glu Glu Met Leu

Ile Gly <210> 115 <211> 1476 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1476) <400> 115

atg aaa Met Lys 1 tac Tyr aaa gac ctg cgc gac ttc atc gcc atg ctc gag cag cag 48 Lys Asp Leu Arg 5 Asp Phe Ile 10 Ala Met Leu Glu Gin 15 Gin ggc aaa ctc aaa cgc atc gcg cac ccc gtt tcc ccg cat ttg gaa atg 96 Gly Lys Leu Lys Arg Ile Ala His Pro Val Ser Pro His Leu Glu Met 20 25 30 acc gaa atc gcc gac cgc gtg ctg cgc gcc gaa ggg ccg gcg ttg ttg 144 Thr Glu Ile Ala Asp Arg Val Leu Arg Ala Glu Gly Pro Ala Leu Leu 35 40 45 ttt gaa cac cca gtt aag ccc gac ggt acg cgc tat gat tat ccc gtg 192 Phe Glu His Pro Val Lys Pro Asp Gly Thr Arg Tyr Asp Tyr Pro Val 50 55 60 ttg gca aac ctg ttc ggc acg ccc gaa cgt gtg gcg atg ggc atg ggc 240 Leu Ala Asn Leu Phe Gly Thr Pro Glu Arg Val Ala Met Gly Met Gly 65 70 75 80 gcg gac age gtg tcc aag ctg cgc gaa atc ggg cag acg ctg gcg tat 288 Ala Asp Ser Val Ser Lys Leu Arg Glu Ile Gly Gin Thr Leu Ala Tyr 85 90 95 ttg aaa gaa CCC gaa ccg ccc aaa ggc att aaa gac gcg ttt tcc aaa 336

230

WO 01/85772

PCT/GB01/02003

Leu Lys Glu Pro 100 Glu Pro Pro Lys Gly 105 Ile Lys Asp Ala Phe 110 Ser Lys ctg ccg ctc ttg aaa gac att tgg agc atg gcg ccg aac gtg gtg aaa 384 Leu Pro Leu 115 Leu Lys Asp Ile Trp 120 Ser Met Ala Pro Asn 125 Val Val Lys aat gcg ccg tgt cag gaa atc gta tgg gaa ggc gaa gac gtt gat ttg 432 Asn Ala 130 Pro Cys Gin Glu Ile 135 Val Trp Glu Gly Glu 140 Asp Val Asp Leu tat caa ctt ccg att cag cat tgc tgg ccg gaa gac gtt gcg ccg ctg 480 Tyr 145 Gin Leu Pro Ile Gin 150 His Cys Trp Pro Glu 155 Asp Val Ala Pro Leu 160 gta acg tgg ggc ttg acc gtc ačg cgc ggg ccg cac aaa aaa cgc caa 528 Val Thr Trp Gly Leu 165 Thr Val Thr Arg Gly 170 Pro His Lys Lys Arg 175 Gin aat ctc ggc att tac cgc caa caa tta atc ggc ata aac aag ctg att 576 Asn Leu Gly Ile ISO Tyr Arg Gin Gin Leu 185 Ile Gly Ile Asn Lys 190 Leu Ile atg cgc tgg ctg tcg cat cgc ggc ggc gcg ctg gat tat cag gaa ttc 624 Met Arg Trp 195 Leu Ser His Arg Gly 200 Gly Ala Leu Asp Tyr 205 Gin Glu Phe cgc aaa ctc aat ccc gat acg ccg tat ccc gtc gcc gtc gtg ctc ggc 672 Arg Lys 210 Leu Asn Pro Asp Thr 215 Pro Tyr Pro Val Ala 220 Val Val Leu Gly tgc gac cct gcc acc att ttg ggc gcg gta acg ccc gtt ccc gat acc 720 Cys 225 Asp Pro Ala Thr Ile 230 Leu Gly Ala Val Thr 235 Pro Val Pro Asp Thr 240 ttg agc gaa tac cag ttt gcc gga ctg ctg cgc ggt tcg cgg acg gaa 768 Leu Ser Glu Tyr Gin 245 Phe Ala Gly Leu Leu 250 Arg Gly Ser Arg Thr 255 Glu ctg gtg aaa tgt atc ggc 'aac gat ttg caa gtg cct gcc cgc gca gaa 816 Leu Val Lys Cys 260 Ile Gly Asn Asp Leu 265 Gin Val Pro Ala Arg 270 Ala Glu atc gtg ttg gaa ggc gtc atc cat cca aac gaa acc gcg ttg gaa ggt 864 Ile Val Leu 275 Glu Gly Val Ile His 280 Pro Asn Glu Thr Ala 285 Leu Glu Gly cca tac ggc gac cac acg ggc tat tac aac gag cag gac cat ttc ccc 912

231

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9 99 9 9 999

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9 9 9 9 9

9999 9999 99 999

PCT/GBO1/02003

Pro Tyr Gly Asp His 290 Thr Gly Tyr Tyr Asn Glu Gin Asp His Phe Pro 295 300 gtg ttt acg gtc gaa cgc atc acc atg cgc gaa aac ccg att tac cac 960 Val Phe Thr Val Glu Arg Ile Thr Met Arg Glu Asn Pro Ile Tyr His 305 310 315 320 tet acc tac aca ggc aaa ccg ccc gat gaa ccc gcc gtt ttg ggc gtg 1008 Ser Thr Tyr Thr Gly Lys Pro Pro Asp Glu Pro Ala Val Leu Gly Val 325 330 335 gcg ttg aac gaa gtg ttc gta ccg ctt ttg caa aag cag ttc ccc gaa 1056 Ala Leu Asn Glu Val Phé val Pro Leu Leu Gin Lys Gin Phe Pro Glu 340 345 350 atc acc gat ttt tac ctg ccg cčc gaa ggc tgc tcc tac cgc atg gcg 1104 Ile Thr Asp Phe Tyr Leu Pro Pro Glu Gly Cys Ser Tyr Arg Met Ala 355 360 365 gtg gtg agc atg aaa aaa cag tac gcc gga cac gcc aag cgc gtg atg 1152 Val Val Ser Met Lys Lys Gin Tyr Ala Gly His Ala Lys Arg Val Met 370 375 380 atg ggc tgc tgg tcg ttc ctg cgc cag ttt atg tac acc aaa ttc atc 1200 Met Gly Cys Trp Ser Phe Leu Arg Gin Phe Met Tyr Thr Lys Phe Ile 385 390 395 400 att gtg gtg gat gac gat gtg gat gtg cgc gac tgg aaa gaa gtc atc 1248 Ile Val Val Asp Asp Asp Val Asp val Arg Asp Trp Lys Glu Val Ile 405 410 415 tgg gcg gta acc acg cgc atg gac ccc gtg cgc gat acc gtt ttg. atg 1296 Trp Ala Val Thr Thr Arg Met Asp Pro Val Arg Asp Thr Val Leu Met 420 425 430 gaa aac acg ccc atc gac tac ctc gac ttc gcc agc ccc gtc agc gga 1344 Glu Asn Thr Pro Ile Asp Tyr Leu Asp Phe Ala Ser Pro Val Ser Gly 435 440 445 ctt ggc ggc aaa atg ggt ttg gat gcg acc aac aag tgg ccg ggc gaa 1392 Leu Gly Gly Lys Met Gly Leu Asp Ala Thr Asn Lys Trp Pro Gly Glu 450 455 460 acc gac cgc gaa tgg gga cgg gtg att aaa aaa gac cct gcg gtt acg 1440 Thr Asp Arg Glu Trp Gly Arg Val Ile Lys Lys Asp Pro Ala Val Thr 465 470 475 480 get aag att gat gag att tgg gag gaa ttg ggg ttg 1476

232 ·· ·· ·♦ · ····♦· • · · * · ♦ ·· · · · • 4 · · » · 4 4

4 «··· » · · · * • 4 4 · ·' -« · · φ ···« ···· ·· ··· ·· 44

WO 01/85772 PCT/GB01/02003

Ala Lys Ile Asp Glu Ile Trp Glu Glu Leu Gly Leu 485 490 <210> 116 <211> 492 <212> PRT <213> Neisseria meningitidis <400> 116

Met 1 Lys Tyr Lys Asp 5 Leu Arg Asp Phe Ile 10 Ala Met Leu Glu Gin 15 Gin Gly Lys Leu Lys 20 Arg Ile Ala His Pro 25 Val Ser Pro His Leu 30 Glu Met Thr Glu Ile 35 Ala Asp Arg Val Leu 40 Arg Ala Glu Gly Pro 45 Ala Leu Leu Phe. Glu 50 His Pro Val Lys Pro 55 Asp Gly Thr Arg Tyr 60 Asp Tyr Pro Val Leu 65 Ala Asn Leu Phe Gly 70 Thr Pro Glu Arg Val 75 Ala Met Gly Met Gly 80 Ala Asp Ser Val Ser 85 Lys Leu Arg Glu Ile 90 Gly Gin Thr Leu Ala 95 Tyr Leu Lys Glu Pro 100 Glu Pro Pro Lys Gly 105 Ile Lys Asp Ala Phe 110 Ser Lys Leu Pro Leu 115 Leu Lys Asp Ile Trp 120 Ser Met Ala Pro Asn 125 Val Val Lys Asn Ala 130 Pro Cys Gin Glu Ile 135 Val Trp Glu Gly Glu 140 Asp Val Asp Leu Tyr 145 Gin Leu Pro Ile Gin 150 His Cys Trp Pro Glu 155 Asp Val Ala Pro Leu 160 Val Thr Trp Gly Leu 165 Thr Val Thr Arg Gly 170 Pro His Lys Lys Arg 175 Gin Asn Leu Gly Ile 180 Tyr Arg Gin Gin Leu 185 Ile Gly Ile Asn Lys 190 Leu Ile Met Arg Trp Leu Ser His Arg Gly Gly Ala Leu Asp Tyr Gin Glu Phe

195 200 205

233 ·· ·«·« ·· <*· • · « • · β « • · ··· ···«

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PCT/GB01/02003

Arg Lys Leu Asn Pro Asp Thr Pro Tyr Pro Val Ala Val Val Leu Gly 210 215 220 Cys Asp Pro Ala Thr Ile Leu Gly Ala Val Thr Pro Val Pro Asp Thr 225 230 235 240 Leu Ser Glu Tyr Gin Phe Ala Gly Leu Leu Arg Gly Ser Arg Thr Glu 245 250 255 Leu Val Lys Cys Ile Gly Asn Asp Leu Gin Val Pro Ala Arg Ala Glu 260 265 270 Ile Val Leu Glu Gly Val Ile His Pro Asn Glu Thr Ala Leu Glu Gly 275 280 285 Pro Tyr Gly Asp His Thr Gly Tyr Tyr Asn Glu Gin Asp His Phe Pro 290 295 300 Val Phe Thr Val Glu Arg Ile Thr Met Arg Glu Asn Pro Ile Tyr His 305 310 315 320 Ser Thr Tyr Thr Gly Lys Pro Pro Asp Glu Pro Ala Val Leu Gly Val 325 330 335 Ala Leu Asn Glu Val Phe Val Pro Leu Leu Gin Lys Gin Phe Pro Glu 340 345 350 Ile Thr Asp Phe Tyr Leu Pro Pro Glu Gly Cys Ser Tyr Arg Met Ala 355 360 365 Val Val Ser Met Lys Lys Gin Tyr Ala Gly His Ala Lys Arg Val Met 370 375 380 Met Gly Cys Trp Ser Phe Leu Arg Gin Phe Met Tyr Thr Lys Phe Ile 385 390 395 400 Ile Val Val Asp Asp Asp Val Asp Val Arg Asp Trp Lys Glu Val Ile 405 410 415 Trp Ala Val Thr Thr Arg Met Asp Pro Val Arg Asp Thr Val Leu Met 420 425 430 Glu Asn Thr Pro Ile Asp Tyr Leu Asp Phe Ala Ser Pro Val Ser Gly 435 440 445 Leu Gly Gly Lys Met Gly Leu Asp Ala Thr Asn Lys Trp Pro Gly Glu

450 455 460

234

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..λ-·:.··/· ,0.:10-0^··· • · · r · · ·· · · · • fc · « · ··· • fcfc·· · Λ · fc · fc e · fc.

• fcfcfc fcfc·· ·· fcfcd

PCT/GB01/02003

Thr Asp Arg Glu Trp Gly Arg Val Ile Lys Lys Asp Pro Ala Val Thr 465 470 475 480

Ala Lys Ile Asp Glu Ile Trp Glu Glu Leu Gly Leu 485 490 <210> 117 <211> 1515 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1515) <400> 117

atg ttg tat ttc aga tac ggt ttt Phe ttg gtt gtt tgg tgt gcg gca ggt Leu Val Val Trp Cys Ala Ala Gly 48 Met Leu 1 Tyr Phe Arg 5 Tyr Gly ¹⁰ 15 gtt tet gcc gcc tat ggg gcg gat gcg ccc gcg att ttg gat gac aag 96 Val Ser Ala Ala 20 Tyr Gly Ala Asp Ala Pro 25 Ala Ile Leu Asp Asp Lys 30 gca ttg ttg cag gtg cag cgg tcg gtg tcg gat aag tgg gcg gaa tcg 144 Ala Leu Leu 35 Gin Val Gin Arg Ser 40 Val Ser Asp Lys Trp Ala Glu Ser 45 gat tgg aaa gtt gac aat gat gcc ccg cgc gtg gtt gac ggg gat ttt 192 Asp Trp 50 Lys Val Asp Asn Asp 55 Ala Pro Arg Val Val Asp Gly Asp Phe ⁶⁰ ttg ttg gcg cat ccg aaa atg ttg gaa cat agt ttg cgc gac gtg ctc 240 Leu Leu 65 Ala His Pro Lys 70 Met Leu Glu His Ser Leu Arg Asp Val Leu 75 80 aac ggc aat cag gcg gat '-ttg atc gct tcg ttg gcg gat ttg tat gcc 288 Asn Gly Asn Gin Ala 85 Asp Leu Ile Ala Ser 90 Leu Ala Asp Leu Tyr Ala 95 aag ctg ccg gat tat gac gcg gtt ttg tac ggc agg gcg cgg gct ttg 336 Lys Leu Pro Asp 100 Tyr Asp Ala Val Leu Tyr 105 Gly Arg Ala Arg Ala Leu 110 ctg gcg aaa ttg gcg gga agg ccg gcg gag gcg gtg gcg cgg tat cgg 384

235 ·· ·· • · · « ·*» ·!»·«

Κ · · ···

WO 01/85772 PCT/GB01/02003 ·♦·· ·*··

Leu Ala Lys 115 Leu Ala Gly Arg Pro 120 Ala Glu Ala Val Ala Arg 125 Tyr Arg gaa ctg cac ggg gaa aat gcg gca gac gag cgg att ttg ctg gat ttg 432 Glu Leu 130 His Gly Glu Asn Ala 135 Ala Asp Glu Arg Ile 140 Leu Leu Asp Leu gcg gcg gcg gag ttt gac gat ttc cgg ctg aag tcg gca gaa agg cat 480 Ala 145 Ala Ala Glu Phe Asp 150 Asp Phe Arg Leu Lys 155 Ser Ala Glu Arg His 160 ttt gcc gag gcg gaa aaa ttg gat ttg ccg gcg ccg gtt ttg gaa aat 528 Phe Ala Glu Ala Glu 165 Lys Leu Asp Leu Pro 170 Ala Pro Val Leu Glu 175 Asn gtg ggg cgt ttt cgg aaa aaa gcg gag ggg ctg acg ggc tgg cgt ttt 576 Val Gly Arg Phe 180 Arg Lys Lys Ala Glu 185 Gly Leu Thr Gly Trp 190 Arg Phe tcg ggc ggc atc agt ccg gcg gtc aat aga aat gcc aat aat gcc gcg 624 Ser Gly Gly 195 Ile Ser Pro Ala Val 200 Asn Arg Asn Ala Asn 205 Asn Ala Ala ccg cag tat tgc cgg caa aac gga ggc cgg cag ata tgc agt gtc agc 672 Pro Gin 210 Tyr Cys Arg Gin Asn 215 Gly Gly Arg Gin Ile 220 Cys Ser val Ser cgg gcg gag cgg gcg gca ggc ttg aat tat gaa atc gag gcg gaa aaa 720 Arg 225 Ala Glu Arg Ala Ala 230 Gly Leu Asn Tyr Glu 235 Ile Glu Ala GlU' Lys 240 ctg acg gcg ttg gca gat aat cat tat ttg ttg ttc cgt tcc aat atc 768 Leu Thr Ala Leu Ala 245 Asp Asn His Tyr Leu 250 Leu Phe Arg Ser Asn 255 Ile ggc ggc acg agc tat tat ttc agt aaa aaa tea gct tat gac gac ggg 816 Gly Gly Thr Ser 260 Tyr Tyr Phe Ser Lys 265 Lys Ser Ala Tyr Asp 270 Asp Gly ttc ggc aga gcg tat ttg ggt tgg cag tat aaa aat gca cgg cag acg 864 Phe Gly Arg 275 Ala Tyr Leu Gly Trp 280 Gin Tyr Lys Asn Ala 285 Arg Gin Thr gcg ggg att ttg ccg ttt tat cag gtg cag ttg tcg ggc agc gac ggc 912 Ala Gly 290 Ile Leu Pro Phe Tyr 295 Gin Val Gin Leu Ser 300 Gly Ser Asp Gly ttt gat gcg aaa aca aaa cgg gta aac aac cgc cgc ctg ccg ccg tat 960

236

WO 01/85772

PCT/GBO1/02003

Phe Asp Ala Lys Thr Lys Arg Val Asn Asn Arg Arg Leu Pro Pro Tyr 320 305 310 315 atg ctg gcg cac gga gtc ggc gtg cag ttg tcc cat act tac cgc cca 1008 Met Leu Ala His Gly Val Gly Val Gin Leu Ser His Thr Tyr Arg Pro 325 330 335 aac ccg gga tgg caa ttt tcg gtc gcg ctg gaa cat tac cgc caa ego 1056 Asn Pro Gly Trp Gin Phe Ser Val Ala Leu Glu His Tyr Arg Gin Arg 340 345 350 tac cgc gaa cag gat agg gcg gaa tac aat aac ggt cgg cag gac ggg 1104 Tyr Arg Glu Gin Asp Arg Ala Glu Tyr Asn Asn Gly Arg Gin Asp Gly 355 360 365 ttt tat gtt tcg tcg gca aaa cgt ttg ggc gaa tcg gca act gtg ttc 1152 Phe Tyr Val Ser Ser Ala Lys Arg Leu Gly Glu Ser Ala Thr Val Phe 370 375 380 ggc ggc tgg cag ttt gtg cgg ttt gtg ccg aaa cgc gaa acg gtg ggc 1200 Gly Gly Trp Gin Phe Val Arg Phe Val Pro Lys Arg Glu Thr Val Gly 385 390 395 400 ggc gcg gtc aat aat gcc gcc tac cgg cgc aac ggt gtt tat gcc ggc 1248 Gly Ala Val Asn Asn Ala Ala Tyr Arg Arg Asn Gly Val Tyr Ala Gly 405 410 415 tgg gcg cag gag tgg cgg cag ttg ggc ggt ttg aac agt cgg gtt tcc 1296 Trp Ala Gin Glu Trp Arg Gin Leu Gly Gly Leu Asn Ser Arg Val Ser 420 425 430 gcg tet tat gcc cgc cgc aac tat aag ggc gtt gcg gct ttc tcg aca 1344 Ala Ser Tyr Ala Arg Arg Asn Tyr Lys Gly Val Ala Ala Phe Ser Thr 435 440 445 gag gcg caa cgc aac cgc gaa tgg aat gtc tcg ctg gct ttg age cac 1392 Glu Ala Gin Arg Asn Arg Glu Trp Asn Val Ser Leu Ala Leu Ser His 450 455 460 gac aag ttg tcg tac aaa 'ggt atc gtg ccc gcg ttg aat tat cgt ttc 1440 Asp Lys Leu Ser Tyr Lys Gly Ile Val Pro Ala Leu Asn Tyr Arg Phe 465 470 475 480 ggc agg acg gaa agt aat gtg ccg tat gcg aaa cgc cgc aac age gag 1488 Gly Arg Thr Glu Ser Asn Val Pro Tyr Ala Lys Arg Arg Asn Ser Glu 485 490 495 gtg ttt gtg tcg gcg gat tgg cgg ttt 1515

237

WO 01/85772

PCT/GB01/02003

Val Phe Val Ser Ala Asp Trp Arg Phe 500 505 <210> 118 <211> 505 <212> PRT <213> Neisseria meningitidis <400> 118 Met 1 Leu Tyr Phe Arg Tyr Gly Phe 5 Leu Val Val Trp Cys Ala Ala Gly 10 15 Val Ser Ala Ala Tyr Gly Ala Asp 20 Ala Pro Ala Ile Leu Asp Asp Lys 25 30 Ala Leu Leu Gin Val Gin Arg Ser 35 40 Val Ser Asp Lys Trp Ala Glu Ser 45 Asp Trp Lys Val Asp Asn Asp Ala 50 55 Pro Arg Val Val Asp Gly Asp Phe 60 Leu 65 Leu Ala His Pro Lys Met Leu 70 Glu His Ser Leu Arg Asp Val Leu 75 80 Asn Gly Asn Gin Ala Asp Leu Ile 85 Ala Ser Leu Ala Asp Leu Tyr Ala 90 95 Lys Leu Pro Asp Tyr Asp Ala Val 100 Leu Tyr Gly Arg Ala Arg Ala Leu 105 110 Leu Ala Lys Leu Ala Gly Arg Pro 115 120 Ala Glu Ala Val Ala Arg Tyr Arg 125 Glu Leu His Gly Glu Asn Ala Ala 130 135 Asp Glu Arg Ile Leu Leu Asp Leu 140 Ala 145 Ala Ala Glu Phe Asp Asp Phe 150. Arg Leu Lys Ser Ala Glu Arg His 155 160 Phe Ala Glu Ala Glu Lys Leu Asp 165 Leu Pro Ala Pro Val Leu Glu Asn 170 175 Val Gly Arg Phe Arg Lys Lys Ala 180 Glu Gly Leu Thr Gly Trp Arg Phe 185 190 Ser Gly Gly Ile ser Pro Ala Val 195 200 Asn Arg Asn Ala Asn Asn Ala Ala 205

238 » · · · · • · · · · • 9 · ·

WO 01/85772

PCT/GBO1/02003

Pro Gin 210 Tyr Cys Arg Gin Asn 215 Gly Gly Arg Gin Ile 220 Cys Ser Val Ser Arg Ala Glu Arg Ala Ala Gly Leu Asn Tyr Glu Ile Glu Ala Glu Lys 225 230 235 240 Leu Thr Ala Leu Ala Asp Asn His Tyr Leu Leu Phe Arg Ser Asn Ile 245 250 255 Gly Gly Thr Ser Tyr Tyr Phe Ser Lys Lys Ser Ala Tyr Asp Asp Gly 260 265 270 Phe Gly Arg Ala Tyr Leu Gly Trp Gin Tyr Lys Asn Ala Arg Gin Thr 275 280 285 Ala Gly Ile Leu Pro Phe Tyr Gin Val Gin Leu Ser Gly Ser Asp Gly 290 295 300 Phe Asp Ala Lys Thr Lys Arg Val Asn Asn Arg Arg Leu Pro Pro Tyr 305 310 315 320 Met Leu Ala His Gly Val Gly Val Gin Leu Ser His Thr Tyr Arg Pro 325 330 335 Asn Pro Gly Trp Gin Phe Ser Val Ala Leu Glu His Tyr Arg Gin Arg 340 345 350 Tyr Arg Glu Gin Asp Arg Ala Glu Tyr Asn Asn Gly Arg Gin Asp Gly 355 360 365 Phe Tyr Val Ser Ser Ala Lys Arg Leu Gly Glu Ser Ala Thr val Phe 370 375 380 Gly Gly Trp Gin Phe Val Arg Phe Val Pro Lys Arg Glu Thr Val Gly 385 390 395 400 Gly Ala Val Asn Asn Ala Ala Tyr Arg Arg Asn Gly Val Tyr Ala Gly 405 410 415 Trp Ala Gin Glu Trp Arg Gin Leu Gly Gly Leu Asn Ser Arg Val Ser 420 425 430 Ala Ser Tyr Ala Arg Arg Asn Tyr Lys Gly Val Ala Ala Phe Ser Thr 435 440 445 Glu Ala Gin Arg Asn Arg Glu Trp Asn Val Ser Leu Ala Leu Ser His

450

455

460

239

WO 01/85772

PCT/GB01/02003

Asp 465 Lys Leu Ser Tyr Lys 470 Gly Xle Val Pro Ala 475 Leu Asn Tyr Arg Phe 480 Gly Arg Thr Glu Ser Asn Val Pro Tyr Ala Lys Arg Arg Asn Ser Glu 485 490 495 Val Phe Val Ser Ala Asp Trp Arg Phe

500

505 <210> 119 <211> 756 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(756) <400> 119

atg gaa acg cat tcg ggc tgt ttt ttg cgg aag acg gta atg aaa gac 48 Met Glu Thr His Ser Gly Cys Phe Leu Arg Lys Thr Val Met Lys Asp 1 5 10 15 gat gtt ttg aaa cag cag gca cac gcg gcg ata cag aag aaa ctg ggc 96 Asp Val Leu Lys Gin Gin Ala His Ala Ala Ile Gin Lys Lys Leu Gly 20 25 30 tac gcg ttc cgc gat att tcg ctt ttg cgg cag gct ttg acg cac agg 144 Tyr Ala Phe Arg Asp Ile Ser Leu Leu Arg Gin Ala Leu Thr His Arg 35 40 45 agc cat cat gcg aag cac aac gag cgg ttc gag ttt gtc ggc gat tcg 192 Ser His His Ala Lys His Asn Glu Arg Phe Glu Phe Val Gly Asp Ser 50 55 60 att ttg aat tat acg gtg gcg cgg atg ctg ttt gac gcg ttt ccg aag 240 Ile Leu Asn Tyr Thr Val' Ala Arg Met Leu Phe Asp Ala Phe Pro Lys 65 70 75 80 ttg acc gag ggc gag ttg tcg cgg ttg cgg gca agt ctg gtc aat gag 288 Leu Thr Glu Gly Glu Leu Ser Arg Leu Arg Ala Ser Leu Val Asn Glu 85 90 95 ggc gtg ctg gcg gaa atg gcg gcg gaa atg aat gtc ggc gac ggc ctg 336 Gly Val Leu Ala Glu Met Ala Ala Glu Met Asn Val Gly Asp Gly Leu

240 • ·

WO 01/85772 PCT/GBO1/02003

100 105 110 tac ttg ggg gcg ggc gag ttg aag agc ggc ggc ttc aga cgg cct tcg 384 Tyr Leu Gly Ala Gly Glu Leu Lys Ser Gly Gly Phe Arg Arg Pro Ser 115 120 125 ata ctg gcg gac gcg atg gag gcg atg ttt gcg gcg gtc agc ttc gat 432 Ile Leu Ala Asp Ala Met Glu Ala Met Phe Ala Ala Val Ser Phe Asp 130 135 140 gcg gat ttc aac acg gcg gaa aag gtg gtg cgc cat ttg ttt gca gaa 480 Ala Asp Phe Asn Thr Ala Glu Lys Val Val Arg His Leu Phe Ala Glu 145 150 155 160 cgc gtc cgg ega gtc gat ttc caa aat cag gca aaa gac ggc aaa act 528 Arg val Arg Arg Val Asp Phe Gin Asn Gin Ala Lys Asp Gly Lys Thr 165 170 175 gct ttg cag gag gcg ttg cag gcg cgc cgt ttc gcc ttg ccg aaa tac 576 Ala Leu Gin GlU Ala Leu Gin Ala Arg Arg Phe Ala Leu Pro Lys Tyr 180 185 190 cgt atc gaa gag caa atc ggc cat gcc aac gac agt atg ttt gtc att 624 Arg Ile Glu Glu Gin Ile Gly His Ala Asn Asp Ser Met Phe Val Ile 195 200 205 tcc tgc gat ttg ggc gaa ctg ggt ttc gtg tgc cgt gcc aaa ggg acg 672 Ser Cys Asp Leu Gly Glu Leu Gly Phe Val Cys Arg Ala Lys Gly Thr 210 215 220 agc cgc aag gcg gcg gag cag gaa gcg gcg aaa gag gct ttg aaa tgg 720 Ser Arg Lys Ala, Ala Glu Gin Glu Ala Ala Lys Glu Ala Leu Lys Trp 225 230 235 240 ctg gaa gag aag ctg ccg ctg aag aag aaa aag aaa 756 Leu Glu Glu Lys Leu Pro Leu Lys Lys Lys Lys Lys

245 250 <210> 120 , <211> 252 <212> PRT <213> Neisseria meningitidis <400> 120

Met Glu Thr His Ser Gly Cys Phe Leu Arg Lys Thr Val Met Lys Asp 15 10 15

241

• • • • · • WO 01/85772 PCT/GBO1/02003 Asp Val Leu Lys Gin Gin Ala His Ala Ala Ile Gin Lys Lys Leu Gly 20 25 30 Tyr Ala Phe Arg Asp Ile Ser Leu Leu Arg Gin Ala Leu Thr His Arg 35 40 45 Ser His His Ala Lys His Asn Glu Arg Phe Glu Phe Val Gly Asp Ser ⁵⁰ 55 60 Ile Leu Asn Tyr Thr Val Ala Arg Met Leu Phe Asp Ala Phe Pro Lys 65 70 75 80 Leu Thr Glu Gly Glu Leu Ser Arg Leu Arg Ala Ser Leu Val Asn Glu 85 90 95 Gly Val Leu Ala Glu Met Ala Ala Glu Met Asn Val Gly Asp Gly Leu 100 105 110 Tyr Leu Gly Ala Gly Glu Leu Lys Ser Gly Gly Phe Arg Arg Pro Ser 115 120 125 Ile Leu Ala Asp Ala Met Glu Ala Met Phe Ala Ala Val Ser Phe Asp 130 135 140 Ala Asp Phe Asn Thr Ala Glu Lys Val Val Arg His Leu Phe Ala Glu 145 150 155 160 Arg Val Arg Arg Val Asp Phe Gin Asn Gin Ala Lys Asp Gly Lys Thr 165 170 175 Ala Leu Gin Glu Ala Leu Gin Ala Arg Arg Phe Ala Leu Pro Lys Tyr 180 185 190 Arg Ile Glu Glu Gin Ile Gly His Ala Asn Asp Ser Met Phe Val Ile 195 200 - 205 Ser Cys Asp Leu Gly Glu Leu Gly Phe Val Cys Arg Ala Lys Gly Thr 210 215 220 Ser Arg Lys Ala Ala Glu Gin Glu Ala Ala Lys Glu Ala Leu Lys Trp 225 230 235 240 Leu Glu Glu Lys Leu Pro Leu Lys Lys Lys Lys Lys

245 250 <210> 121

242

WO 01/85772

PCT/GBO1/02003 <211> 570 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(570) <400> 121

atg gac aat cac gcc gaa gca cac tgg caa aac ggc tgg ctt caa age 48 Met 1 Asp Asn His Ala 5 Glu Ala His Trp Gin Asn Gly Trp Leu Gin Ser 10 ¹⁵ ata cgc cat acc ccg tcg ccc aat ttc age ccg agg gaa acg ggg gaa 96 Ile Arg His Thr Pro Ser Pro Asn Phe Ser Pro Arg Glu Thr Gly Glu 20 25 30 acg gtt tcc ctg atc gtg ttg cac aac att tea ctg ccg ccg ttc gaa 144 Thr Val Ser Leu Ile Val Leu His Asn Ile Ser Leu Pro Pro Phe Glu 35 40 45 tac ggc acg gat get gtg gaa aag ctg ttt gcc aac cgg ctc gac ccc 192 Tyr Gly Thr Asp Ala Val Glu Lys Leu Phe Ala Asn Arg Leu Asp Pro 50 55 60 aac gga cat ccg ttc ttc age ctg ata cac act ttg cgc gta tcc age 240 Asn Gly His Pro Phe Phe Ser Leu Ile His Thr Leu Arg Val Ser Ser 65 70 75 80 cat ttc tta atc aaa cgc gac ggc aaa acg gtg cag ttc gta tea tgc 288 His Phe Leu Ile Lys Arg Asp Gly Lys Thr Val Gin Phe Val Ser Cys 85 90 95 ggc gat atg gcg tac cac gcg ggc gta tcc tcg ttt cgc gga cgg gaa 336 Gly Asp Met Ala Tyr His Ala Gly Val Ser Ser Phe Arg Gly Arg Glu 100 105 110 aaa tgc aac gca ttt tcc atc ggc atc gaa ttg gaa ggc tgc gat ttc 384 Lys Cys Asn Ala Phe Ser Ile Gly Ile Glu Leu Glu Gly Cys Asp Phe 115 120 125 gaa ccc ttt acc gaa gcg caa tac cgt tcg ctc gaa aca ttg ttg gaa 432 Glu Pro Phe Thr Glu Ala Gin Tyr Arg Ser Leu Glu Thr Leu Leu Glu 130 135 140 gca ctc tgc cgc cgc tac ccc gtt acc gca gta acc gga cat cag gac 480 Ala Leu Cys Arg Arg Tyr Pro val Thr Ala Val Thr Gly His Gin Asp 145 150 155 160

243

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atc gcg ccc ggc cgc Ile Ala Pro Gly Arg aaa Lys acc gac Thr Asp ccc ggc cac ttt ttc gac tgg cgg Pro Gly His 170 Phe Phe Asp Trp 175 Arg 165 cgg ata cgg gag aaa ggg ttt CCC gta gac aga aat gcc gtc Arg Ile Arg Glu Lys Gly Phe Pro Val Asp Arg Asn Ala Val 180 185 190

<210> 122 <211> 190 <212> PRT <213> Neisseria meningitidis <400> 122

Met 1 Asp Asn His Ala 5 Glu Ala His Trp Gin 10 Asn Gly Trp Leu Gin 15 Ser Ile Arg His Thr 20 Pro Ser Pro Asn Phe 25 Ser Pro Arg Glu Thr 30 Gly Glu Thr Val Ser 35 Leu Ile Val Leu His 40 Asn Ile Ser Leu Pro 45 Pro Phe Glu Tyr Gly 50 Thr Asp Ala Val Glu 55 Lys Leu Phe Ala Asn 60 Arg Leu Asp Pro Asn 65 Gly His Pro Phe Phe 70 Ser Leu Ile His Thr 75 Leu Arg Val Ser Ser 80 His Phe Leu Ile Lys 85 Arg Asp Gly Lys Thr 90 Val Gin Phe Val Ser 95 Cys Gly Asp Met Ala 100 Tyr His Ala Gly Val 105 Ser Ser Phe Arg Gly 110 Arg Glu Lys Cys Asn 115 Ala Phe Ser Ile Gly 120 Ile Glu Leu Glu Gly 125 Cys Asp Phe Glu Pro 130 Phe Thr Glu Ala Gin 135 Tyr Arg Ser Leu Glu 140 Thr Leu Leu Glu Ala 145 Leu Cys Arg Arg Tyr 150 Pro Val Thr Ala Val 155 Thr Gly His Gin Asp 160 Ile Ala Pro Gly Arg 165 Lys Thr Asp Pro Gly 170 His Phe Phe Asp Trp 175 Arg

244 ·· 44 44

44 ·44 4

WO 01/85772 PCT/GB01/02003

Arg Ile Arg Glu Lys Gly Phe Pro Val Asp Arg Asn Ala Val 180 185 190 <210> 123 <211> 2274 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1) . .(2274) <400> 123

atg Met 1 aca aca tta cat Leu His 5 ttc Phe tca ggc ttc ccg cgt gtc ggt gcc ttc cgc 48 Thr Thr Ser Gly Phe Pro Arg 10 Val Gly Ala Phe 15 Arg gaa ttg aaa ttc gca caa gaa aaa tac tgg cgc aaa gaa atc age gag 96 Glu Leu Lys Phe Ala Gin Glu Lys Tyr Trp Arg Lys Glu Ile Ser Glu 20 25 30 caa gaa ttg ctg gct gtt gct aaa gac ttg cgc gag aaa aac tgg aaa 144 Gin Glu Leu Leu Ala Val Ala Lys Asp Leu Arg Glu Lys Asn Trp Lys 35 40 45 cac cag gcc gct gcc aac gcc gat tac gtt gcc gta ggc gat ttc act 192 His Gin Ala Ala Ala Asn Ala Asp Tyr Val Ala Val Gly Asp Phe Thr 50 55 60 ttc tac gac cac atc ctc gac ctg caa gtc gcc acc ggc gcg att ccc 240 Phe Tyr Asp His Ile Leu Asp Leu Gin Val Ala Thr Gly Ala Ile Pro 65 70 75 80 gcc cgc ttc ggc ttc gac age caa aac cta tet ttg gaa caa ttc ttc 288 Ala Arg Phe Gly Phe Asp Ser Gin Asn Leu Ser Leu Glu Gin Phe Phe 85 90 95 caa ctg gcg cgc ggt aac aaa gac caa ttc gct atc gaa atg acc aaa 336 Gin Leu Ala Arg Gly Asn Lys Asp Gin Phe Ala Ile Glu Met Thr Lys 100 105 110 tgg ttc gac acc aac tac cac tac ttg gtg cct gaa ttc cac gcc gat 384 Trp Phe Asp Thr Asn Tyr His Tyr Leu Val Pro Glu Phe His Ala Asp 115 120 125

245 ·· ·r· ·

94«· «··· · • ·«·· «·· • ·«·· ···· · • · ··· · · · · ··«· ···· *« ·«· _Φ·

WO 01/85772 PCT/GBO1/02003 acc gaa ttt aaa gcc aat gcc aaa cac tac gtt caa cag ctg caa gaa 432 Thr Glu 130 Phe Lys Ala Asn Ala 135 Lys His Tyr Val Gin 140 Gin Leu Gin Glu gcc caa get ttg ggc ctg aaa gcc aaa ccg acc gtt gtg ggt ccg ttg 480 Ala 145 Gin Ala Leu Gly Leu 150 Lys Ala Lys Pro Thr 155 Val Val Gly Pro Leu 160 act ttc ctg tgg gtc ggt aaa gaa aaa ggc gcc gtt gaa ttc gac cgt 528 Thr Phe Leu Trp Val 165 Gly Lys Glu Lys Gly 170 Ala Val Glu Phe Asp 175 Arg ctg age ctg ttg cct aaa ctg ttg cct gtt tac gtt gaa atc ctg act 576 Leu Ser Leu Leu 180 Pro Lys Leu Leu Pro 185 Val Tyr Val Glu Ile 190 Leu Thr get ttg gtt gaa gcc ggt gcc gag tgg att caa atc gac gag cct get 624 Ala Leu Val 195 Glu Ala Gly Ala Glu 200 Trp Ile Gin Ile Asp 205 Glu Pro Ala ttg act gtt gac ctg cct aaa gaa tgg gta gaa gca tac aaa gac gtt 672 Leu Thr 210 Val Asp Leu Pro Lys 215 Glu Trp Val Glu Ala 220 Tyr Lys Asp Val tac gcc act ttg age aaa gta agt gcc aaa atc ctg ctg age act tac 720 Tyr 225 Ala Thr Leu Ser Lys 230 Val Ser Ala Lys Ile 235 Leu Leu Ser Thr Tyr 240 ttc ggt tet gtt gcc gaa cac gcc gca ttg ttg aaa tcc ctg cct gtt 768 Phe Gly Ser Val Ala 245 Glu His Ala Ala Leu 250 Leu Lys Ser Leu Pro 255 Val gac ggc ctg cac atc gac ttg gta cgc get cct gaa caa ctg gat gcg 816 Asp Gly Leu His 260 Ile Asp Leu Val Arg 265 Ala Pro Glu Gin Leu 270 Asp Ala ttc gcc gac tac gac aaa gtt ctg tet gcc ggc gtc att gac ggc cgc 864 Phe Ala Asp 275 Tyr Asp Lys Val Leu 280 Ser Ala Gly Val Ile 285 Asp Gly Arg aat att tgg cgc gcc aac ctg aac aaa gtt ttg gaa act gtt gag ctt 912 Asn Ile 290 Trp Arg Ala Asn Leu 295 Asn Lys Val Leu Glu 300 Thr Val Glu Leu ctg caa gcc aaa ctg ggc gac cgt ttg tgg att tcc age tet tgc tcg 960 Leu 305 Gin Ala Lys Leu Gly 310 Asp Arg Leu Trp Ile 315 Ser Ser Ser Cys Ser 320

246

·· • « >4 • ♦ · • 4« 4 ·· • 4 44· • • 4 4 « « 4 4 • • • B 4 4 4 • 4 · • ·*· ·· ··· 4 4 ··

WO 01/85772

PCT/GB01/02003 i

ctg ctg cac act ccg ttt gac ttg tca gtt gaa gaa aaa ctg aaa gcc 1008 Leu Leu His The Pro Phe Asp Leu Ser Val 330 Glu Glu Lys Leu Lys Ala 335 325 aac aaa ccc gac ctg tac tet tgg ttg gca ttc acc ctg caa aaa acc 1056 Asn Lys Pro Asp Leu Tyr Ser Trp Leu Ala Phe Thr Leu Gin Lys Thr 340 345 350 caa gaa ttg cgc gtc ctg aaa gcc gca ttg aac gaa ggc cgt gat tet 1104 Gin Glu Leu Arg Val Leu Lys Ala Ala Leu Asn Glu Gly Arg Asp Ser 355 360 365 gtt gcc gaa gaa ctg get gcc age caa get get gcc gac tcc cgt gcc 1152 Val Ala Glu Glu Leu Ala Ala Ser Gin Ala Ala Ala Asp Ser Arg Ala 370 375 380 aac age age gaa atc cac cgt gca gac gtt gcc aaa cgc ctg gcc gat 1200 Asn Ser Ser Glu Ile His Arg Ala Asp Val Ala Lys Arg Leu Ala Asp 385 390 395 400 ttg cct gcc aac gca gac caa cgc aaa tcc cca ttt gcc gac cgt atc 1248 Leu Pro Ala Asn Ala Asp Gin Arg Lys Ser Pro Phe Ala Asp Arg Ile 405 410 415 aaa gcg caa caa gca tgg ttg aac ctg cct ctg ctg ccg acg acc aac 1296 Lys Ala Gin Gin Ala Trp Leu Asn Leu Pro Leu Leu Pro Thr Thr Asn 420 425 430 atc ggt tet ttc ccg caa act acc gaa atc cgc caa gca cgc gca gcc 1344 Ile Gly Ser Phe Pro Gin Thr Thr Glu Ile Arg Gin Ala Arg Ala Ala 435 440 445 ttc aaa aaa ggc gaa ctg tet gcc gcc gat tac gaa gcc gcg atg aaa 1392 Phe Lys Lys Gly Glu Leu Ser Ala Ala Asp Tyr Glu Ala Ala Met Lys 450 455 460 aaa gaa atc gcc ttg gtg gtt gaa gag caa gaa aaa ctg gac ttg gac 1440 Lys Glu Ile Ala Leu Val Val Glu Glu Gin Glu Lys Leu Ašp Leu Asp 465 470 475 480 gta ctg gta cac ggc gaa gcc gag cgt aac gac atg gtt gaa tac ttc 1488 Val Leu Val His Gly Glu Ala Glu Arg Asn Asp Met Val Glu Tyr Phe 485 490 495 ggc gaa ttg ttg age ggt ttt gca ttc acc caa tat ggc tgg gta caa 1536 Gly Glu Leu Leu Ser Gly Phe Ala Phe Thr Gin Tyr Gly Trp Val Gin 500 505 510

247

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agc tac ggt tet cgc tgc gtt aaa Cys Val Lys 520 cca ccg att atc ttc ggt gac gta Asp Val 1584 Ser Tyr Gly Ser Arg 515 Pro Pro Ile Ile Phe •525 Gly agc cgt cct gaa gcc atg acc gta gct tgg tet act tac gca caa agc 1632 Ser Arg Pro Glu Ala Met Thr Val Ala Trp Ser Thr Tyr Ala Gin Ser 530 535 540 ctg acc aaa cgc ccg atg aaa ggt atg ttg acc ggc cct gta acc att 1680 Leu Thr Lys Arg Pro Met Lys Gly Met Leu Thr Gly Pro Val Thr Ile 545 550 555 560 ctg caa tgg tet ttc gtc cgc aac gat att cct cgc tet acc gtg tgt 1728 Leu Gin Trp Ser Phe Val Arg Asn Asp Ile Pro Arg Ser Thr Val Cys 565 570 575 aaa caa atc gca ctg gct ctg aac gac gaa gta ttg gat ctg gaa aaa 1776 Lys Gin Xle Ala Leu Ala Leu Asn Asp Glu Val Leu Asp Leu Glu Lys 580 585 590 gca ggc atc aaa gtc atc caa atc gac gaa cct gcc atc cgc gaa ggc 1824 Ala Gly Xle Lys Val Ile Gin Ile Asp Glu Pro Ala Ile Arg Glu Gly 595 600 605 ctg cct ttg aaa cgt gcc gat tgg gat gcc tac ctg aac tgg gcc ggc 1872 Leu Pro Leu Lys Arg Ala Asp Trp Asp Ala Tyr Leu Asn Trp Ala Gly 610 • 615 620 gaa tet ttc cgc ctg tcc tet acc ggt tgc gaa gac agc act caa atc 1920 Glu Ser Phe Arg Leu Ser Ser Thr Gly Cys Glu Asp Ser Thr Gin Ile 625 630 635 640 cac acc cat atg tgc tac tet gag ttc aac gac atc ctg cct gcg att 1968 His Thr His Met Cys Tyr Ser Glu Phe Asn Asp Ile Leu Pro Ala Ile 645 650 655 gct gca atg gat gcg gac gtg atc acc atc gag act tca cgt tcc gac 2016 Ala Ala Met Asp Ala Asp Val Ile Thr Ile Glu Thr Ser Arg Ser Asp 660 - 665 670 atg gaa ctc ttg acc gcg ttc ggc gaa ttc aaa tac ccg aac gac atc 2064 Met Glu Leu Leu Thr Ala Phe Gly Glu Phe Lys Tyr Pro Asn Asp Ile 675 680 685 ggc ccg ggg gtt tac gac atc cac agc ccg cgc gta ccg aca gaa gcc 2112 Gly Pro Gly Val Tyr Asp Ile His Ser Pro Arg Val Pro Thr Glu Ala 690 695 700 •

248

WO 01/85772

gaa gtg gag cac ctg ttg Glu 705 Val Glu His Leu Leu 710 cgt ctg tgg gtt aac ccg Arg Leu Trp Val Asn 725 Pro gaa act ctg gaa caa ctc Glu Thr Leu Glu Gin Leu

740

cgc aaa gcc atc gag gtt Arg Lys Ala Xle Glu 715 Val gac tgc ggc ctg aaa aca Asp Cys Gly Leu 730 Lys Thr caa gtg atg atg aac gta Gin Val Met 745 Met Asn Val

PCT/GBO1/02003 gta ccg gtt gaa 2160 Val Pro Val Glu 720 cgc ggc tgg aaa 2208 Arg Gly Trp 735 Lys acc cac aaa ttg 2256 Thr His Lys Leu

750

cgt gcc gaa ttg gcg aaa 2274 Arg Ala Glu 755 Leu Ala Lys

<210> 124 <211> 758 <212> PRT <213> Neisseria meningitidis <400> 124

Met 1 Thr Thr Leu His Phe Ser Gly Phe Pro Arg Val Gly Ala Phe Arg 5 10 15 Glu Leu Lys Phe Ala Gin Glu Lys Tyr Trp Arg Lys Glu Ile Ser Glu 20 25 30 Gin Glu Leu Leu Ala Val Ala Lys Asp Leu Arg Glu Lys Asn Trp Lys 35 40 45 His Gin Ala Ala Ala Asn Ala Asp Tyr Val Ala Val Gly Asp Phe Thr 50 55 60 Phe Tyr Asp His Xle Leu Asp Leu Gin Val Ala Thr Gly Ala Ile Pro 65 70 75 80 Ala Arg Phe Gly Phe Asp Ser Gin Asn Leu Ser Leu Glu Gin Phe Phe 85 90 95 Gin Leu Ala Arg Gly Asn Lys Asp Gin Phe Ala Ile Glu Met Thr Lys 100 105 110 Trp Phe Asp Thr Asn Tyr His Tyr Leu Val Pro Glu Phe His Ala Asp 115 120 125 Thr Glu Phe Lys Ala Asn Ala Lys His Tyr Val Gin Gin Leu Gin Glu

249

WO 01/85772

130

Ala Gin Ala 145

The Phe Leu

Leu Sec Leu

Ala Leu Val 195

Leu Thr Val 210

Tyr Ala Thr 225

Phe Gly Ser

Asp Gly Leu

Phe Ala Asp 275

Asn Ile Trp 290

Leu Gin Ala 305

Leu Leu His

Asn Lys Pro

Gin Glu Leu 355

Val Ala Glu 370

Asn Ser Ser

PCT/GB01/02003

135 140 Leu Gly Leu Lys Ala Lys Pro Thr Val Val Gly Pro Leu 150 155 160 Trp Val Gly Lys Glu Lys Gly Ala Val Glu Phe Asp Arg 165 170 175 Leu Pro Lys Leu Leu Pro Val Tyr Val Glu Ile Leu Thr 180 185 190 Glu Ala Gly Ala Glu Trp Ile Gin Ile Asp Glu Pro Ala 200 205 Asp Leu Pro Lys Glu Trp Val Glu Ala Tyr Lys Asp Val 215 ' 220 Leu Ser Lys Val Ser Ala Lys Ile Leu Leu Ser Thr- Tyr 230 235 240 Val Ala Glu His Ala Ala Leu Leu Lys Ser Leu Pro Val 245 250 255 His Ile Asp Leu Val Arg Ala Pro Glu Gin Leu Asp Ala 260 265 270 Tyr Asp Lys Val Leu Ser Ala Gly Val Ile Asp Gly Arg 280 285 Arg Ala Asn Leu Asn Lys Val Leu Glu Thr Val Glu Leu 295 300 Lys Leu Gly Asp Arg Leu Trp Ile Ser Ser Ser Cys Ser 310 315 320 Thr Pro Phe Asp Leu Ser Val Glu Glu Lys Leu Lys Ala 325 330 335 Asp Leu Tyr Ser Trp Leu Ala Phe Thr Leu Gin Lys Thr 340 '' 345 350 Arg Val Leu Lys Ala Ala Leu Asn Glu Gly Arg Asp Ser 360 365 Glu Leu Ala Ala Ser Gin Ala Ala Ala Asp Ser Arg Ala 375 380 Glu Ile His Arg Ala Asp Val Ala Lys Arg Leu Ala Asp

250

9 9

WO 01/85772 PCT/GB01/02003 385 390 395 400

Leu Pro Ala Asn Ala 405 Asp Gin Arg Lys Ser 410 Pro Phe Ala Asp Arg 415 Ile Lys Ala Gin Gin 420 Ala Trp Leu Asn Leu 425 Pro Leu Leu Pro Thr 430 Thr Asn Xle Gly Ser 435 Phe Pro Gin Thr. Thr 440 Glu Ile Arg Gin Ala 445 Arg Ala Ala Phe Lys 450 Lys Gly Glu Leu Ser 455 Ala Ala Asp Tyr Glu 460 Ala Ala Met Lys Lys 465 Glu Ile Ala Leu Val 470 Val Glu Glu Gin Glu 475 Lys Leu Asp Leu Asp 480 Val Leu Val His Gly 485 Glu Ala Glu Arg Asn 490 Asp Met Val Glu Tyr 495 Phe Gly Glu Leu Leu 500 ser Gly Phe Ala Phe 505 Thr Gin Tyr Gly Trp 510 Val Gin Ser Tyr Gly 515 Ser Arg Cys Val Lys 520 Pro Pro Ile Ile Phe 525 Gly Asp Val Ser Arg 530 Pro Glu Ala Met Thr 535 Val Ala Trp Ser Thr 540 Tyr Ala Gin Ser Leu 545 Thr Lys Arg Pro Met 550 Lys Gly Met Leu Thr 555 Gly Pro Val Thr Ile 560 Leu Gin Trp Ser Phe 565 Val Arg Asn Asp Ile 570 Pro Arg Ser Thr Val 575 Cys Lys Gin Ile Ala 580 Leu Ala Leu Asn Asp 585 Glu Val Leu Asp Leu 590 Glu Lys Ala Gly Ile 595 Lys Val Ile Gin Ile 600 Asp Glu Pro Ala Ile 605 Arg Glu Gly Leu Pro 610 Leu Lys Arg Ala Asp 615 Trp Asp Ala Tyr Leu 620 Asn Trp Ala Gly Glu 625 Ser Phe Arg Leu Ser 630 Ser Thr Gly Cys Glu 635 Asp Ser Thr Gin Ile 640 His Thr His Met Cys Tyr Ser Glu Phe Asn Asp Ile Leu Pro Ala Ile

251

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PCT/GBO1/02003

645 650 655 Ala Ala Met Asp 660 Ala Asp Val Ile Thr 665 Ile Glu Thr Ser Arg 670 Ser Asp Met Glu Leu 675 Leu Thr Ala Phe Gly 680 Glu Phe Lys Tyr Pro 685 Asn Asp Ile Gly Pro 690 Gly Val Tyr Asp Ile 695 His Ser Pro Arg Val 700 Pro Thr Glu Ala Glu 705 Val Glu His Leu Leu 710 Arg Lys Ala Ile Glu 715 Val Val Pro Val Glu 720 Arg Leu Trp Val Asn 725 Pro Asp Cys Gly Leu 730 Lys Thr Arg Gly Trp 735 Lys Glu Thr Leu Glu 740 Gin Leu Gin Val Met 745 Met Asn Val Thr His 750 Lys Leu Arg Ala Glu 755 Leu Ala Lys

<210> 125 <211> 1137 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1137) <400> 125

atg agc cgt tta tgg ttt ttt gcc gca aaa aac att ata atc cgc ctt 48 Met Ser Arg Leu Trp Phe Phe Ala Ala Lys Asn Ile Ile Ile Arg Leu 1 5 10 15 att tac cta ttg ccc aag 'gag aca caa atg gca ctc gta tcc atg cgc 96 Ile Tyr Leu Leu Pro Lys Glu Thr Gin Met Ala Leu Val Ser Met Arg 20 25 30 caa ctg ctt gat cat get gcc gaa aac agc tac ggc ctg ccc gcg ttc 144 Gin Leu Leu Asp His Ala Ala Glu Asn Ser Tyr Gly Leu Pro Ala Phe 35 40 45 aac gtc aac aac ctc gaa caa atg cgc gcc att atg gaa gcc gcc gac 192

252 tax

WO 01/85772

PCT/GBO1/02003

Asn Val ⁵⁰ Asn Asn Leu Glu Gin 55 Met Arg Ala Ile Met 60 Glu Ala Ala Asp caa gtc aac gcg ccc gtc atc gta cag gcg age gca ggt gcg cgc aaa 240 Gin 65 Val Asn Ala Pro Val 70 Ile Val Gin Ala Ser 75 Ala Gly Ala Arg Lys 80 tac gcg ggc gcg ccg ttt ttg cgc cac ctg att ttg gcg get gtc gaa 288 Tyr Ala Gly Ala Pro 85 Phe Leu Arg His Leu 90 Ile Leu Ala Ala Val 95 Glu gaa ttt ccg cac atc ccč gtc gtg atg cac caa gac cac ggc gca tcg 336 Glu Phe Pro His 100 Ile Pro Val Val Met 105 His Gin Asp His Gly 110 Ala Ser ccc gac gtg tgc caa cgc tcc atc caa ctg ggc ttt tcc tcc gtg atg 384 Pro Asp Val 115 Cys Gin Arg Ser Ile 120 Gin Leu Gly Phe Ser 125 Ser Val Met atg gac ggc tcg ctg atg gaa gac ggc aaa acc cct tet tet tat gaa 432 Met Asp 130 Gly Ser Leu Met Glu 135 Asp Gly Lys Thr Pro 140 Ser Ser Tyr Glu tac aac gtc aac gcc acc cgt acc gtg gtt aat ttc tcc cac gcc tgc 480 Tyr 145 Asn Val Asn Ala Thr 150 Arg Thr Val Val Asn 155 Phe Ser His Ala Cys 160 ggc gta tcc gtt gaa ggc gaa atc ggc gta ttg ggc aac ctc gaa act 528 Gly Val Ser Val Glu 165 Gly Glu Ile Gly Val 170 Leu Gly Asn Leu Glu 175 Thr ggc gaa gcc ggc gaa gaa gac ggt gta ggc gca gtg ggc aaa ctt tcc 576 Gly Glu Ala Gly 180 GlU Glu Asp Gly Val 185 Gly Ala Val Gly Lys 190 Leu Ser cac gac caa atg ctc acc age gtc gaa gat gcc gtg cgt ttc gtt aaa 624 His Asp Gin 195 Met Leu Thr Ser Val 2Ó0 Glu Asp Ala Val Arg 205 Phe val Lys gat acc ggc gtt gac gca \ttg gcg att gcc gtc ggc acc age cac ggc 672 Asp Thr 210 Gly Val Asp Ala Leu 215 Ala Ile Ala Val Gly 220 Thr Ser His Gly gcg tac aaa ttc acc cgt ccg ccc aca ggc gac gtg ttg cgt atc gac 720 Ala 225 Tyr Lys Phe Thr Arg 230 Pro Pro Thr Gly Asp 235 Val Leu Arg Ile Asp 240 cgc atc aaa gaa atc cac caa gcc ctg ccc aat aca cac atc gtg atg 768

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Arg Ile Lys Glu Ile 245 His Gin Ala Leu Pro Asn Thr His Ile Val Met 250 255 cac ggc tcc age tcc gtt ccg caa gaa tgg ctg aaa gtc atc aac gaa 816 His Gly Ser Ser Ser Val Pro Gin Glu Trp Leu Lys Val Ile Asn Glu ²⁶⁰ 265 270 tac ggc ggc aat atc ggc gaa acc tac ggc gtg ccg gtt gaa gaa atc 864 Tyr Gly Gly Asn Ile Gly Glu Thr Tyr Gly Val Pro Val Glu Glu Ile 275 280 285 gtc gaa ggc atc aaa cac ggc gtg cgt aaa gtc aac atc gat acc gac 912 Val Glu Gly Ile Lys His Gly Val Arg Lys Val Asn Ile Asp Thr Asp 290 295 300 ttg cgc ctt gct tcc acc ggc gcg gta cgc cgc tac ctt gcc gaa aac 960 Leu Arg Leu Ala Ser Thr Gly Ala Val Arg Arg Tyr Leu Ala Glu Asn 305 310 315 320 ccg tcc gac ttc gat ccg cgc aaa tat ttg age aaa acc att gaa gcg 1008 Pro Ser Asp Phe Asp Pro Arg Lys Tyr Leu Ser Lys Thr Ile Glu Ala 325 330 335 atg aag caa atc tgc ctc gac cgc tac ctc gcg ttc ggt tgc gaa ggt 1056 Met Lys Gin Ile Cys Leu Asp Arg Tyr Leu Ala Phe Gly Cys Glu Gly 340 345 350 cag gca ggc aaa atc aaa ccg gtt tcc ttg gaa aaa atg gca aac cgt 1104 Gin Ala Gly Lys Ile Lys Pro Val Ser Leu Glu Lys Met Ala Asn Arg 355 360 365 tat gcc aag ggc gaa ttg aac caa atc gtc aaa 1137 Tyr Ala Lys Gly Glu Leu Asn Gin Ile Val Lys 370 375

<210> 126 <211> 379 <212> PRT <213> Neisseria meningitidis <400> 126 Met Ser Arg Leu Trp Phe Phe Ala Ala Lys Asn Ile Ile Ile Arg Leu 15 10 15

Ile Tyr Leu Leu Pro Lys Glu Thr Gin Met Ala Leu Val Ser Met Arg 20 25 30

254

κ.

f • · · · ·

WO 01/85772

Gin Leu Leu Asp 35

Asn Val Asn Asn 50

Gin Val Asn Ala 65

Tyr Ala Gly Ala

PCT/GB01/02003

Glu Phe Pro His 100

Pro Asp Val Cys 115

Met Asp Gly Ser 130

Tyr Asn Val Asn 145

Gly Val Ser Val

Gly Glu Ala Gly 180

His Asp Gin Met 195

Asp Thr Gly Val 210

Ala Tyr Lys Phe 225

Arg Ile Lys Glu

His Gly Ser Ser 260

Tyr Gly Gly Asn 275

His Ala Ala Glu 40 Asn Ser Tyr Gly Leu 45 Pro Ala Phe Leu Glu Gin 55 Met Arg Ala Ile Met 60 Glu Ala Ala Asp Pro Val 70 Ile Val Gin Ala Ser 75 Ala Gly Ala Arg Lys 80 Pro 85 Phe Leu Arg His Leu 90 Ile Leu Ala Ala Val 95 Glu Ile Pro Val Val Met 105 His Gin Asp His Gly 110 Ala Ser Gin Arg Ser Ile 120 Gin Leu Gly Phe Ser 125 Ser Val Met Leu Met Glu 135 Asp Gly Lys Thr Pro 140 Ser Ser Tyr Glu Ala Thr 150 Arg Thr Val Val Asn 155 Phe Ser His Ala Cys 160 Glu 165 Gly Glu Ile Gly Val 170 Leu Gly Asn Leu Glu 175 Thr Glu Glu Asp Gly Val 185 Gly Ala Val Gly Lys 190 Leu Ser Leu Thr Ser Val 200 Glu Asp Ala Val Arg 205 Phe Val Lys Asp Ala Leu 215 Ala Ile Ala Val Gly 220 Thr Ser His Gly Thr Arg 230 Pro Pro Thr Gly Asp 235 Val Leu Arg Ile Asp 240 Ile 245 His _xGln Ala Leu Pro 250 Asn Thr His Ile Val 255 Met Ser Val Pro Gin Glu 265 Trp Leu Lys Val Ile 270 Asn Glu Ile Gly Glu Thr Tyr Gly Val Pro Val Glu Glu Ile

280 285

255 • ' • ·

WO 01/85772

Val Glu Gly Ile Lys His Gly Val Arg Lys Val Asn Ile 290 295 300

Leu Arg Leu Ala Ser Thr Gly Ala Val Arg Arg Tyr Leu 305 310 315

Pro Ser Asp Phe Asp Pro Arg Lys Tyr Leu Ser Lys Thr 325 330

Met Lys Gin Ile Cys Leu Asp Arg Tyr Leu Ala Phe Gly 340 345

Gin Ala Gly Lys Ile Lys Pro Val Ser Leu Glu Lys Met 355 360 365

Tyr Ala Lys Gly Glu Leu Asn Gin Ile Val Lys 370 375

PCT/GBO1/02003

Asp Thr Asp

Ala Glu Asn 320

Ile Glu Ala 335

Cys Glu Gly 350

Ala Asn Arg <210> 127 <211> 444 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(444) <400> 127

gtg gat gct gca gca Ala 5 acg Thr ctc Leu ctg aca tac aaa tgc ccg gcg gaa aac 48 Val Asp Ala 1 Ala Leu Thr Tyr 10 Lys Cys Pro Ala Glu 15 Asn cgc cca aac aca aaa aaa att ggt aat ttt tcc tat tea agg cta caa 96 Arg Pro Asn Thr Lys Lys Ile Gly Asn Phe Ser Tyr Ser Arg Leu Gin 20 25 30 ttc gac acg cac agg gca tet gcc ega ttt cac aat cat aat agc gga 144 Phe Asp Thr His Arg Ala Ser Ala Arg Phe His Asn His Asn Ser Gly 35 40 45 gtt aaa aat atg aca gct aaa gga caa atg ttg caa gat ccc ttc ctg 192 Val Lys Asn Met Thr Ala Lys Gly Gin Met Leu Gin Asp Pro Phe Leu 50 55 60 aac gcc ctg cgt aaa gag cat gtt ccg gtt tcg att tac tta gtt aac 240 Asn Ala Leu Arg Lys Glu His Val Pro Val Ser Ile Tyr Leu Val Asn

256

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65 70 75 80 ggt atc aaa ttg caa ggt cag gtt gag tet ttc gat caa tac gtt gtt 288 Gly Ile Lys Leu Gin Gly Gin Val Glu Ser Phe Asp Gin Tyr Val Val 85 90 95 ctc ctg aga aac act tcc gtc acc caa atg gtt tac aaa cac gcc att 336 Leu Leu Arg Asn Thr Ser Val Thr Gin Met Val Tyr Lys His Ala Ile 100 105 110 tcc acc atc gta ccg gca cgc tcc gtc aac cta caa cat gaa aac aga 384 Ser Thr Ile Val Pro Ala Arg Ser Val Asn Leu Gin His Glu Asn Arg 115 120 125 ccc caa gcc gca ccg act tcg acc ctc gtc caa gtg gaa acc gtc cag 432 Pro Gin Ala Ala Pro Thr Ser Thr Leu Val Gin Val Glu Thr Val Gin 130 135 140 cag cct gcc gaa 444 Gin Pro Ala Glu

145 <210> 128 <211> 148 <212> PRT

<213> Neisseria meningitidis <400> 128 Val Asp Ala Ala Ala Thr Leu Leu Thr Tyr Lys Cys Pro Ala Glu Asn 1 5 10 15 Arg Pro Asn Thr Lys Lys Ile Gly Asn Phe Ser Tyr Ser Arg Leu Gin 20 25 30 Phe Asp Thr His Arg Ala Ser Ala Arg Phe His Asn His Asn Ser Gly 35 40 45 Val Lys Asn Met Thr Ala Lys Gly Gin Met Leu Gin Asp Pro Phe Leu 50 . 55 60 Asn Ala Leu Arg Lys Glu His Val Pro Val Ser Ile Tyr Leu Val Asn 65 70 75 80 Gly Ile Lys Leu Gin Gly Gin Val Glu Ser Phe Asp Gin Tyr Val Val 85 90 95 Leu Leu Arg Asn Thr Ser Val Thr Gin Met Val Tyr Lys His Ala Ile

257 • ·

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100 105 110

Ser Thr Ile Val Pro Ala Arg Ser Val Asn Leu Gin His Glu Asn Arg 115 120 125 Pro Gin Ala Ala Pro Thr Ser Thr Leu Val Gin Val Glu Thr Val Gin 130 135 140 Gin Pro Ala Glu 145

<210> 129 <211> 1308 <212> DNA <213> Neisseria meningitidis <22 0>

<221> CDS <222> (1)..(1308) <400> 129 atg tac get aaa aaa ggc ggt ttg gga ctg gtt aaa age cgc cgt ttc 48

Met Tyr Ala Lys Lys Gly Gly Leu Gly Leu Val Lys Ser Arg Arg Phe

15 10 15 gca cct ctt ttc get acg cag ttt ctc ggc gcg ttc aac gac aat gtg 96

Ala Pro Leu Phe Ala Thr Gin Phe Leu Gly Ala Phe Asn Asp Asn Val

20 25 30 ttc aaa acc gcg ctg ttt gtg atg att ggg ttt tac ggt ttg ggg caa 144

Phe Lys Thr Ala Leu Phe Val Met Ile Gly Phe Tyr Gly Leu Gly Gin

35 40 45 aac ggc ttc ctg cct gcc gga cag atg ttg aac ttg ggc gcg ttg ctg 192

Asn Gly Phe Leu Pro Ala Gly Gin Met Leu Asn Leu Gly Ala Leu Leu

50 55 60 ttt att ttg ccg tat ttc 'ctg ttt tcc tcg ctg tcg ggg cag ttg ggt 240

Phe Ile Leu Pro Tyr Phe Leu Phe Ser Ser Leu Ser Gly Gin Leu Gly

65 70 75 80 aac aaa ttc gac aag gcc gtt ttg gcg cgt tgg gcc aag gtg ctg gaa 288

Asn Lys Phe Asp Lys Ala Val Leu Ala Arg Trp Ala Lys Val Leu Glu

85 90 95 atg atc att atg gcg gtg gcg gca tac ggg ttt tat atc cgg tet gcc 336

258 • ·

WO 01/85772 PCT/GB01/02003

Met Ile Ile Met 100 Ala Val Ala Ala Tyr 105 Gly Phe Tyr Ile Arg 110 Ser Ala ccg ctg ctt ttg gcg tgt ctg ttt tgc atg ggc gcg caa tcg acg ctg 384 Pro Leu Leu 115 Leu Ala Cys Leu Phe 120 Cys Met Gly Ala Gin 125 Ser Thr Leu ttc ggg ccg ctg aaa tac gcc atc ctg ccc gat tat ctc gac gac aaa 432 Phe Gly 130 Pro Leu Lys Tyr Ala 135 Ile Leu Pro Asp Tyr 140 Leu Asp Asp Lys gag ttg atg atg ggc aac agc ctg att gaa tcg ggt acg ttt gtc gcc 480 Glu 145 Leu Met Met Gly Asn 150 Ser Leu Ile Glu Ser 155 Gly Thr Phe Val Ala 160 atc ctg ttc ggt cag ata ctg ggg act gcg gtg gca ggt gta ccg cct 528 Ile Leu Phe Gly Gin 165 Ile Leu Gly Thr Ala 170 Val Ala Gly Val Pro 175 Pro tat att gtc ggg ata ctg gtt ttg ctg gtc gcc gta gga ggc acg gtc 576 Tyr Ile Val Gly 180 Ile Leu Val Leu Leu 185 Val Ala Val Gly Gly 190 Thr Val ggc agc ctg ttt atg ccg tcc gta ccc •gcc aag gct gcc gat aca caa 624 Gly Ser Leu 195 Phe Met Pro Ser Val 200 Pro Ala Lys Ala Ala 205 Asp Thr Gin att gag tgg aat att gtc cgg ggt aca aaa tcc ctg ctg cgt gaa acg 672 Ile Glu 210 Trp Asn Ile Val Arg 215 Gly Thr Lys Ser Leu 220 Leu Arg Glu Thr gtg cgg cac aag ccc gtt ttt acc gcc att atc ggt att tcg tgg ttt 720 Val 225 Arg His Lys Pro Val 230 Phe Thr Ala Ile Ile 235 Gly Ile Ser Trp Phe 240 tgg ttt gtc ggc gcg gtt tat acc acg caa ctg ccg acc ttt acc caa 768 Trp Phe Val Gly Ala 245 Val Tyr Thr Thr Gin 250 Leu Pro Thr Phe Thr 255 Gin atc cat cta ggc ggc aac. • gac aat gtt ttc aac ctg atg ctt gcc ctg 816 Ile His Leu Gly 260 Gly Asn Asp Asn Val 265 Phe Asn Leu Met Leu 270 Ala Leu ttt tcc atc ggt att gcc gcc ggt tcg gta ctg tgt gcc aag ttc agc 864 Phe Ser Ile 275 Gly Ile Ala Ala Gly 280 ser Val Leu Cys Ala 285 Lys Phe Ser agg gaa cgg ctg agg ttg gct tgg gta acg gtt ggt gcg ttg ggt ttg 912

259

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Arg Glu 290 Arg Leu Arg Leu Ala 295 Trp Val Thr Val Gly 300 Ala Leu Gly Leu acg gtt tgc ggc ttg gtt ttg gtg tgg ctg acg cac gga cac cgt ttt 960 Thr 305 Val Cys Gly Leu Val 310 Leu Val Trp Leu Thr 315 His Gly His Arg Phe 320 gaa ggg ctg aac ggc att ttt tgg ttt tta tcg caa gga tgg gca tat 1008 Glu Gly Leu Asn Gly 325 Ile Phe Trp Phe Leu 330 Ser Gin Gly Trp Ala 335 Tyr ccc gtg atg gcg gtg atg acg ctg atc ggc ttt ttc ggc gga ttt ttc 1056 Pro Val Met Ala 340 Val Met Thr Leu Ile 345 Gly Phe Phe Gly Gly 350 Phe Phe tcc gtt ccg ctc tat acc tgg ctg caa acc gcc agt age gag act ttc 1104 Ser Val Pro 355 Leu Tyr Thr Trp Leu 360 Gin Thr Ala Ser Ser 365 Glu Thr Phe cgc gcc cgc gcc gtt gcc gcc aac aat atc gtt aac ggt att ttt atg 1152 Arg Ala 370 Arg Ala Val Ala Ala 375 Asn Asn Ile Val Asn 380 Gly Ile Phe Met gtt tcc get gcc gtt ttg age gcg gtg ttg ctg ttt ttg ttt gac age 1200 Val 385 Ser Ala Ala Val Leu 390 Ser Ala Val Leu Leu 395 Phe Leu Phe Asp Ser 400 att tcc ttg ttg tat ctg att gtc get ttg ggc aat att ccg ttg tcg 1248 Ile Ser Leu Leu Tyr 405 Leu Ile Val Ala Leu 410 Gly Asn Ile Pro Leu 415 Ser gta ttt ttg att aag cgc gaa agg cgg ttt tta ggc gcg gcg gca atc 1296 Val Phe Leu Ile 420 Lys Arg Glu Arg Arg 425 Phe Leu Gly Ala Ala 430 Ala Ile

agg aaa aaa cct 1308

Arg Lys Lys Pro

435 <210> 130 <211> 436 <212> PRT <213> Neisseria meningitidis <400> 130

Met Tyr Ala Lys Lys Gly Gly Leu Gly Leu Val Lys Ser Arg Arg Phe 15 10 15

260 ·· ·· • » · ' · • · ·· • « · • « ·· »·ύ·

WO 01/85772 PCT/GB01/02003 ···· ····

Ala Pro Leu Phe 20 Ala Thr Gin Phe Leu 25 Gly Ala Phe Asn Asp 30 Asn Val Phe Lys Thr 35 Ala Leu Phe Val Met 40 Ile Gly Phe Tyr Gly 45 Leu Gly Gin Asn Gly 50 Phe Leu Pro Ala Gly 55 Gin Met Leu Asn Leu 60 Gly Ala Leu Leu Phe 65 Ile Leu Pro Tyr Phe 70 Leu Phe Ser Ser Leu 75 Ser Gly Gin Leu Gly 80 Asn Lys Phe Asp Lys 85 Ala Val Leu Ala Arg 90 Trp Ala Lys Val Leu 95 Glu Met Ile Ile Met 100 Ala Val Ala Ala Tyr 105 Gly Phe Tyr Ile Arg 110 Ser Ala Pro Leu Leu 115 Leu Ala Cys Leu Phe 120 Cys Met Gly Ala Gin 125 Ser Thr Leu Phe Gly 130 Pro Leu Lys Tyr Ala 135 Ile Leu Pro Asp Tyr 140 Leu Asp Asp Lys Glu 145 Leu Met Met Gly Asn 150 Ser Leu Ile Glu Ser 155 Gly Thr Phe Val Ala 160 Ile Leu Phe Gly Gin 165 Ile Leu Gly Thr Ala 170 Val Ala Gly Val Pro 175 Pro Tyr Ile Val Gly 1Θ0 Ile Leu Val Leu Leu 185 Val Ala Val Gly Gly 190 Thr Val Gly Ser Leu 195 Phe Met Pro Ser Val 200 Pro Ala Lys Ala Ala 205 Asp Thr Gin Ile Glu 210 Trp Asn Ile Val Arg 215 Gly Thr Lys Ser Leu 220 Leu Arg Glu Thr Val 225 Arg His Lys Pro Val 230 Phe Thr Ala Ile Ile 235 Gly Ile Ser Trp Phe 240 Trp Phe Val Gly Ala 245 val Tyr Thr Thr Gin 250 Leu Pro Thr Phe Thr 255 Gin Ile His Leu Gly 260 Gly Asn Asp Asn Val 265 Phe Asn Leu Met Leu 270 Ala Leu

251

9· ·· ·* • 4 · · · · • · · · β · · · · • · · · ·«·· ···· ··

4« ····

WO 01/85772 PCT/GBO1/02003

Phe Ser Ile 275 Gly Ile Ala Ala Gly 280 Ser Val Leu Cys Ala 285 Lys Phe Ser Arg Glu 290 Arg Leu Arg Leu Ala 295 Trp Val Thr Val Gly 300 Ala Leu Gly Leu Thr 305 Val Cys Gly Leu Val 310 Leu Val Trp Leu Thr 315 His Gly His Arg Phe 320 Glu Gly Leu Asn Gly 325 Ile Phe Trp Phe Leu 330 Ser Gin Gly Trp Ala 335 Tyr Pro Val Met Ala 340 Val Met Thr Leu Ile 345 Gly Phe Phe Gly Gly 350 Phe Phe Ser Val Pro 355 Leu Tyr Thr Trp Leu 360 Gin Thr Ala Ser Ser 365 Glu Thr Phe Arg Ala 370 Arg Ala Val Ala Ala 375 Asn Asn Ile Val Asn 380 Gly Ile Phe Met Val 385 Ser Ala Ala Val Leu 390 Ser Ala Val Leu Leu 395 Phe Leu Phe Asp Ser 400 Ile Ser Leu Leu Tyr 405 Leu Ile Val Ala Leu 410 Gly Asn Ile Pro Leu 415 Ser Val Phe Leu Ile Lys Arg Glu Arg Arg Phe Leu Gly Ala Ala Ala Ile

420 425 430

Arg Lys Lys Pro 435 <210> 131 <211> 876 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(876) <400> 131 atg aat tac gca aaa gaa atc aat gcg tta aat aac age ctt tcc gat 48

Met Asn Tyr Ala Lys Glu Ile Asn Ala Leu Asn Asn Ser Leu Ser Asp

262

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1 5 10 15 ttg aaa ggc gac atc aac gtt tcg ttt gaa ttt ttt cca ccg aaa aac 96 Leu Lys Gly Asp Ile Asn Val Ser Phe Glu Phe Phe Pro Pro Lys Asn 20 25 30 gag caa atg gaa acg atg ctg tgg gat tcc atc cac cgt ctg caa acc 144 Glu Gin Met Glu Thr Met Leu Trp Asp Ser Ile His Arg Leu Gin Thr 35 40 45

ctg cat Leu His ccc aag ttc gta tcc gta acc tac ggc gca aac tcc ggc gaa 192 Pro Lys Phe Val Ser Val Thr 55 Tyr Gly Ala 60 Asn Ser Gly Glu 50 cgc gac cgc acg cac ggc atc gtc aaa cgc atc aaa cag gaa acc ggc 240 Arg Asp Arg Thr His Gly Ile Val Lys Arg Ile Lys Gin Glu Thr Gly 65 70 75 80 ttg gaa gca gca ccg cac ctg acc ggc atc gac gca tcc ccc gac gaa 288 Leu Glu Ala Ala Pro His Leu Thr Gly Ile Asp Ala Ser Pro Asp Glu 85 90 95 ttg cgc caa atc gcc aaa gac tat tgg gac age ggc atc cgc cgc att 336 Leu Arg Gin Ile Ala Lys Asp Tyr Trp Asp Ser Gly Ile Arg Arg Ile 100 105 110 gtc gcc ctg cgt ggc gac gag ccg ccc ggt tat gag aaa aaa ccg ttt 384 Val Ala Leu Arg Gly Asp Glu Pro Pro Gly Tyr Glu Lys Lys Pro Phe 115 120 125 tac gcc gaa gac ttg gtt aag cta tta cgc tcc gtc gcc gac ttc gac 432 Tyr Ala Glu Asp Leu Val Lys Leu Leu Arg Ser Val Ala Asp Phe Asp 130 135 140 atc tet gtg gcg gca tat ccc gaa gtg cat ccc gaa gcc aaa tcc gca 480 Ile Ser Val Ala Ala Tyr Pro Glu Val His Pro Glu Ala Lys Ser Ala 145 150 155 160 caa gcc gat ctg att aat ctg aag cgc aaa atc gat gcg ggt gca aac 528 Gin Ala Asp Leu Ile Asn v Leu Lys Arg Lys Ile Asp Ala Gly Ala Asn 165 170 175 cac gtc atc acc caa ttt ttc ttt gac gta gaa cgc tac ctg cgc ttc 576 His Val Ile Thr Gin Phe Phe Phe Asp Val Glu Arg Tyr Leu Arg Phe 180 185 190 cgc gac cgc tgc gtg atg ttg ggt atc gat gtg gaa atc gtc cct ggt 624 Arg Asp Arg Cys Val Met Leu Gly Ile Asp Val Glu Ile Val Pro Gly

263

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195 200 att ttg cct gtt acc aac ttc aag Xle Leu Pro Val Thr Asn Phe Lys 210 215 acc aac gtc aaa atc cca agc tgg Thr Asn Val Lys Ile Pro Ser Trp 225 230 gac gac gac caa ggc acg cgc aac Asp Asp Asp Gin Gly Thr Arg Asn 245 gat atg gtc aaa gtc ctg tcc cgc Asp Met Val Lys Val Leu Ser Árg 260 tac acg ctc aac cgc agc gag ctg Tyr Thr Leu Asn Arg Ser Glu Leu 275 280 ggc gtg cgc cct Gly val Arg Pro 290

205 PCT/GB01/02003 cag ctc ggc aaa atg gcg caa gta 672 Gin Leu Gly Lys 220 Met Ala Gin Val ctg tcg caa atg tat gaa ggt ttg 720 Leu Ser Gin 235 Met Tyr Glu Gly Leu 240 ctc gtc gcc gcc agt atc gcc atc 768 Leu Val 250 Ala Ala Ser Ile Ala 255 Ile gaa ggc gtg aaa gat ttc cac ttc 816 Glu 265 Gly val Lys Asp Phe 270 His Phe act tac gcc atc tgc cat att tta 864 Thr Tyr Ala Ile Cys 285 His Ile Leu 876

<210> 132 <211> 292 <212> PRT

<213> Neisseria <400> 132 meningitidis Met 1 Asn Tyr Ala Lys 5 Glu Ile Asn Ala Leu 10 Asn Asn Ser Leu Ser 15 Asp Leu Lys Gly Asp 20 Ile Asn Val Ser Phe 25 Glu Phe Phe Pro Pro 30 Lys Asn Glu Gin Met Glu 35 Thr Meť Leu Trp 40 Asp Ser Ile His Arg 45 Leu Gin Thr Leu His 50 Pro Lys Phe Val Ser 55 Val Thr Tyr Gly Ala 60 Asn Ser Gly Glu Arg 65 Asp Arg Thr His Gly 70 Ile Val Lys Arg Ile 75 Lys Gin Glu Thr Gly 80

264

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• · • · • • · • • • • • ·· • * 9 • · 9 • · • • • • • · · · • • • • • '· • • • • · • · · · • · · • • · ··· • · • ·

Leu Glu Ala Ala Pro 85 His Leu Thr Gly Ile 90 Asp Ala Ser Pro Asp 95 Glu Leu Arg Gin Ile 100 Ala Lys Asp Tyr Trp 105 Asp Ser Gly Ile Arg 110 Arg Ile Val Ala Leu 115 Arg Gly Asp Glu Pro 120 Pro Gly Tyr Glu Lys 125 Lys Pro Phe Tyr Ala 130 Glu Asp Leu Val Lys 135 Leu Leu Arg Ser Val 140 Ala Asp Phe Asp Ile 145 Ser Val Ala Ala Tyr 150 Pro Glu Val His Pro 155 Glu Ala Lys Ser Ala 160 Gin Ala Asp Leu Ile 165 Asn Leu Lys Arg Lys 170 Ile Asp Ala Gly Ala 175 Asn His Val Ile Thr 180 Gin Phe Phe Phe Asp 185 Val Glu Arg Tyr Leu 190 Arg Phe Arg Asp Arg 195 Cys Val Met Leu Gly 200 Ile Asp Val Glu Ile 205 Val Pro Gly Ile Leu 210 Pro Val Thr Asn Phe 215 Lys Gin Leu Gly Lys 220 Met Ala Gin Val Thr 225 Asn Val Lys Ile Pro 230 Ser Trp Leu Ser Gin 235 Met Tyr Glu Gly Leu 240 Asp Asp Asp Gin Gly 245 Thr Arg Asn Leu Val 250 Ala Ala Ser Ile Ala 255 Ile Asp Met Val Lys 260 Val Leu Ser Arg Glu 265 Gly Val Lys Asp Phe 270 His Phe Tyr Thr Leu Asn Arg Ser Glu Leu Thr Tyr Ala Ile Cys His Ile Leu

275 280 285

Gly Val Arg Pro 290 <210> 133 <211> 1371 <212> DNA <213> Neisseria meningitidis

265

WO 01/85772 PCT/GB01/02003 <220>

<221> CDS <222> (1) . .(1371) <400> 133

atg Met 1 agt aat cca ttt tcc tet tta ggt ttg ggt acg gaa ctc gtt tcc 48 Ser Asn Pro Phe 5 Ser Ser Leu Gly Leu Gly 10 Thr Glu Leu Val 15 Ser gca ctg acc gcg caa ggt tac gaa aac ccg aca ccc atc caa gcc gcc 96 Ala Leu Thr Ala Gin Gly Tyr Glu Asn Pro Thr Pro Ile Gin Ala Ala 20 25 30 gcc att ccc aaa gca ctc gcc ggt cat gat ttg cta gcc gcc gcg caa 144 Ala Ile Pro Lys Ala Leu Ala Gly His Asp Leu Leu Ala Ala Ala Gin 35 40 45 acc ggc aca ggc aaa acc gcc gcc ttt atg ctg ccc agt ctg gaa cgc 192 Thr Gly Thr Gly Lys Thr Ala Ala Phe Met Leu Pro Ser Leu Glu Arg 50 55 60 ctc aaa cgt tac gcc acc gcc agc acc tcg ccc gcg atg cac ccc gtg 240 Leu Lys Arg Tyr Ala Thr Ala Ser Thr Ser Pro Ala Met His Pro Val 65 70 75 80 cgt atg ctc gtc ctc ácc ccc acg cgc gaa ctt gcc gac caa atc gac 288 Arg Met Leu Val Leu Thr Pro Thr Arg Glu Leu Ala Asp Gin Ile Asp 85 90 95 caa aac gtg cag ggc tac atc aaa aac ctg ccg ctg cgc cac acc •gtc 336 Gin Asn Val Gin Gly Tyr Ile Lys Asn Leu Pro Leu Arg His Thr Val 100 105 110 ttg ttc ggc ggt atg aat atg gac aaa cag acc gcc gac ctg cgt gcc 384 Leu Phe Gly Gly Met Asn Met Asp Lys Gin Thr Ala Asp Leu Arg Ala 115 120 125 ggc tgc gaa atc gtc gtc gcc acc gtc gga cgg ctg ctc gac cac gtg 432 Gly Cys Glu Ile Val Val' Ala Thr Val Gly Arg Leu Leu Asp His Val 130 135 140 aaa cag aaa aac atc cat ttg aac aaa gtc gaa atc gtc gtt ttg gac 480 Lys Gin Lys Asn Ile His Leu Asn Lys Val Glu Ile Val Val Leu Asp 145 150 155 160 gaa gcc gac cgt atg ctg gat atg ggt ttt atc gac gac atc cgc aaa 528 Glu Ala Asp Arg Met Leu Asp Met Gly Phe Ile Asp Asp Ile Arg Lys

266

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165 170 175 atc atg cag atg ctg ccc cgc caa cgc caa acc ctg ctc ttt tcc Ile Met Gin Met Leu Pro Arg Gin Arg Gin Thr Leu Leu Phe Ser 180 185 190 acc ttc tcc gcc ccg ata cgc aaa ctg gcg caa gac ttc atg aac Thr Phe Ser Ala Pro Ile Arg Lys Leu Ala Gin Asp Phe Met Asn 195 200 205 ccc gaa acc gtc gaa gtc gcc gcg caa gac acc acc aac gcc aac Pro Glu Thr Val Glu Val Ala Ala Gin Asp Thr Thr Asn Ala Asn 210 215 220 gag cag cac atc atc gcc gtc gat acc att cag aag cgc aac ctg Glu Gin His Ile Ile Ala Val Asp Thr Ile Gin Lys Arg Asn Leu 225 230 235 gaa cgg ctg att gtc gat ttg cat atg aac cag gtc atc gtg ttc Glu Arg Leu Ile Val Asp Leu His Met Asn Gin Val Ile Val Phe 245 250 255 aaa acc aaa caa agc gtc gac cgc gta acg cgc gaa ctg gtg cgc Lys Thr Lys Gin Ser Val Asp Arg Val Thr Arg Glu Leu Val Arg 260 265 270 aac ctg tcc gca cag gcg ata cac ggc gac cgt tcc caa caa agc Asn Leu Ser Ala Gin Ala Ile His Gly Asp Arg Ser Gin Gin Ser 275 280 285 ctc gaa aca ctc aac gcc ttc aaa gac ggc aac ctg cgc gtc ctc Leu Glu Thr Leu Asn Ala Phe Lys Asp Gly Asn Leu Arg Val Leu 290 295 300 gcc acc gat atc gcc gcg cgg ggg ctg gat att gcc gaa ctg ccc Ala Thr Asp Ile Ala Ala Arg Gly Leu Asp Ile Ala Glu Leu Pro 305 310 315 gtc atc aat tac gaa atg ccc gcc cag ccc gaa gac tac gtc cac Val Ile Asn Tyr Glu Met. .Pro Ala Gin Pro Glu Asp Tyr Val His 325 330 335 atc ggg cgc acg ggg cgc gcg ggc gcg gac ggc gtg gcg att tcc Ile Gly Arg Thr Gly Arg Ala Gly Ala Asp Gly Val Ala Ile Ser 340 345 350 atg gac gaa tcc gaa cag aaa atg ttt gaa tcc att aaa gag ctg Met Asp Glu Ser GlU Gin Lys Met Phe Glu Ser Ile Lys Glu Leu

gcc 576 Ala gcg 624 Ala gtc 672 Val ctc 720

Leu

240 tgc 768 Cys cgc 816 Arg cgg 864 Arg gtc 912 Val ttc 960

Phe

320 cgc 1008 Arg ctg 1056 Leu acc 1104 Thr

267

WO 01/85772 PCT/GB01/02003 355 360 365 ggc aac aag ctg ctc atc gag cgc atc gag ggc ttc gag ccg caa tgg 1152 Gly Asn Lys Leu Leu Ile Glu Arg Ile Glu Gly Phe Glu Pro Gin’ Trp 370 375 380 tgg gaa cag ggc ggc gca aaa ccg gaa aaa ccc gaa atg cgc gaa cca 1200 Trp Glu Gin Gly Gly Ala Lys Pro Glu Lys Pro Glu Met Arg Glu Pro 385 390 395 400 aga caa cgc aac cgc tac gaa tcc gcc aaa gcg caa cgc gaa aaa aac 1248 Arg Gin Arg Asn Arg Tyr Glu Ser Ala Lys Ala Gin Arg Glu Lys Asn 405 410 415 acc cgg ccg gaa aat gcg gca aac gát gcg ggc gcg get tgc gga aaa 1296 Thr Arg Pro Glu Asn Ala Ala Asn Asp Ala Gly Ala Ala Cys Gly Lys 420 425 430 att gcc gga cgc agc cgc ega agc cgc cgg gaa cac cgg acg tgc gcc 1344 Ile Ala Gly Arg Ser Arg Arg Ser Arg Arg Glu His Arg Thr Cys Ala 435 440 445 ctg ctc caa ccg cgt tac ggc gta aaa 1371 Leu Leu Gin Pro Arg Tyr Gly Val Lys 450 455 <210> 134 <211> 457 <212> PRT <213> Neisseria meningitidis <400> 134 Met Ser Asn Pro Phe Ser Ser Leu Gly Leu Gly Thr Glu Leu Val.Ser 1 5 10 15 Ala Leu Thr Ala Gin Gly Tyr Glu Asn Pro Thr Pro Ile Gin Ala Ala 20 25 30 Ala Ile Pro Lys Ala Leu'Ala Gly His Asp Leu Leu Ala Ala Ala Gin 35 40 45 Thr Gly Thr Gly Lys Thr Ala Ala Phe Met Leu Pro Ser Leu Glu Arg 50 55 60 Leu Lys Arg Tyr Ala Thr Ala Ser Thr Ser Pro Ala Met His Pro Val 65 70 75 80

268 •······· • ·

WO 01/85772 - PCT/GB01/02003 Arg Met Leu Val Leu 85 Thr Pro Thr Arg Glu 90 Leu Ala Asp Gin Ile 95 Asp Gin Asn Val Gin 100 Gly Tyr Ile Lys Asn 105 Leu Pro Leu Arg His 110 Thr Val Leu Phe Gly 115 Gly Met Asn Met Asp 120 Lys Gin Thr Ala Asp 125 Leu Arg Ala Gly Cys 130 Glu Ile Val Val Ala 135 Thr Val Gly Arg Leu 140 Leu Asp His Val Lys 145 Gin Lys Asn Ile His 150 Leu Asn Lys Val Glu 155 Ile Val Val Leu Asp 160 Glu Ala Asp Arg Met 165 Leu Asp Met Gly Phe 170 Ile Asp Asp Ile Arg 175 Lys Ile Met Gin Met 180 Leu Pro Arg Gin Arg 185 Gin Thr Leu Leu Phe 190 Ser Ala Thr Phe Ser ¹⁹⁵ Ala Pro Ile Arg Lys 200 Leu Ala Gin Asp Phe 205 Met Asn Ala Pro Glu 210 Thr Val Glu Val Ala 215 Ala Gin Asp Thr Thr 220 Asn Ala Asn Val Glu 225 Gin His Ile Ile Ala 230 Val Asp Thr Ile Gin 235 Lys Arg Asn Leu Leu 240 Glu Arg Leu Ile Val 245 Asp Leu His Met Asn 250 Gin Val Ile Val Phe 255 Cys Lys Thr Lys Gin 260 Ser Val Asp Arg Val 265 Thr Arg Glu Leu Val 270 Arg Arg Asn Leu Ser 275 Ala Gin Ala Ile His 280 Gly Asp Arg Ser Gin 285 Gin Ser Arg Leu Glu 290 Thr Leu Asn Ala sPhe 295 Lys Asp Gly Asn Leu 300 Arg Val Leu Val Ala 305 Thr Asp Ile Ala Ala 310 Arg Gly Leu Asp Ile 315 Ala Glu Leu Pro Phe 320 Val Ile Asn Tyr Glu 325 Met Pro Ala Gin Pro 330 Glu Asp Tyr Val His 335 Arg

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Ile Gly Arg Thr 340 Gly Arg Ala Gly Ala 345 Asp Gly Val Ala Ile 350 Ser Leu Met Asp Glu 355 Ser Glu Gin Lys Met 360 Phe Glu Ser Ile Lys 365 Glu Leu Thr Gly Asn 370 Lys Leu Leu Ile Glu 375 Arg Ile Glu Gly Phe 380 Glu Pro Gin Trp Trp 385 Glu Gin Gly Gly Ala 390 Lys Pro Glu Lys Pro 395 Glu Met Arg Glu Pro 400 Arg Gin Arg Asn Arg 405 Tyr Glu Ser Ala Lys 410 Ala Gin Arg Glu Lys 415 Asn Thr Arg Pro Glu 420 Asn Ala Ala Asn Asp 425 Ala Gly Ala Ala Cys 430 Gly Lys Ile Ala Gly Arg 435 Ser Arg Arg Ser 440 Arg Arg Glu His Arg 445 Thr Cys Ala Leu Leu 450 Gin Pro Arg Tyr Gly 455 Val Lys

<210> 135 <211> 1374 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1374) <400> 135 atg aaa cac atc cac att atc ggt atc ggc ggc acg ttt atg ggt ggg

Met Lys His Ile His Ile Ile Gly Ile Gly Gly Thr Phe Met Gly Gly

15 10 15 att gcc gcc att gcc aaa gaa gca ggg ttt gaa gtc agc ggt tgc gat

Ile Ala Ala Ile Ala Lys Glu Ala Gly Phe Glu Val Ser Gly Cys Asp

20 25 30 gcg aag atg tat ccg ccg atg agc acc cag ctc gaa gcc ttg ggc ata

Ala Lys Met Tyr Pro Pro Met Ser Thr Gin Leu Glu Ala Leu Gly Ile

35 40 45

144

270 • · ········ ·· ·

WO 01/85772

PCT/GB01/02003

ggc gtg tat gaa ggc ttc gac acc gcg cag ttg gac. gaa ttt aaa gcc Ala 192 Gly Val 50 Tyr Glu Gly Phe Asp 55 Thr Ala Gin Leu Asp Glu 60 Phe Lys gac gtt tac gtt atc ggc aat gtc gcc aag cgc ggg atg gat gtg gtt 240 Asp Val Tyr Val Ile Gly Asn Val Ala Lys Arg Gly Met Asp Val Val 65 70 75 80 gaa gcg att ttg aac cgt ggg ctg cct tat att tcc ggc ccg caa tgg 288 Glu Ala Ile Leu Asn Arg Gly Leu Pro Tyr Ile Ser Gly Pro Gin Trp 85 90 95 ctg gct gaa aac gtg ctg cac cat cat tgg gta ctc ggc gtg gcg ggg 336 Leu Ala Glu Asn Val Leu His His His Trp Val Leu Gly Val Ala Gly 100 105 110 acg cac ggc aaa acg acc acc gcg tet atg ctc gcg tgg gtt ttg gaa 384 Thr His Gly Lys Thr Thr Thr Ala Ser Met Leu Ala Trp Val Leu Glu 115 120 125 tat gcc gga ctc gca ccg ggc ttc ctt atc ggc ggc gta ccg gaa aac 432 Tyr Ala Gly Leu Ala Pro Gly Phe Leu Ile Gly Gly Val Pro Glu Asn 130 135 140 ttc age gtt tcc gcc cgc ctg ccg caa acg ccg cgc caa gac ccg aac 480 Phe Ser Val Ser Ala Arg Leu Pro Gin Thr Pro Arg Gin Asp Pro Asn 145 150 155 160 age caa tcg ccg ttt ttc gtc att gaa gcc gac gaa tac gac acc gcg 528 Ser Gin ser Pro Phe Phe Val ile Glu Ala Asp Glu Tyr Asp Thr Ala 165 170 175 ttt ttc gac aaa cgc tcc aaa ttc gtg cat tac cgt ccg cgt acc gcc 576 Phe Phe Asp Lys Arg Ser Lys Phe Val His Tyr Arg Pro Arg Thr Ala 180 185 190 gtg ttg aac aat ctg gaa ttc gac cac gcc gac atc ttc gcc gat ttg 624 Val Leu Asn Asn Leu Glu Phe Asp His Ala Asp Ile Phe Ala Asp Leu 195 200 205 ggc gcg ata cag acc cag ttc cac cac ctc gtg cgt acc gtg ccg tet 672 Gly Ala Ile Gin Thr Gin Phe His His Leu Val Arg Thr Val Pro Ser 210 215 220 gaa ggc ctc atc gtc tgc aac gga cgg cag caa age ctg caa gac act 720 Glu Gly Leu Ile Val Cys Asn Gly Arg Gin Gin Ser Leu Gin Asp Thr 225 230 235 240

271

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ttg gac aaa ggc tgc tgg acg ccg gtg gaa aaa ttc ggc acg gaa cac 768 Leu Asp Lys Gly Cys Trp Thr Pro Val Glu 250 Lys Phe Gly Thr Glu 255 His 245 ggc tgg cag gcc ggc gaa gcc aat gcc gat ggc tcg ttc gac gtg ttg 816 Gly Trp Gin Ala Gly Glu Ala Asn Ala Asp Gly Ser Phe Asp val Leu 260 265 270 ctt gac ggc aaa aaa gcc gga cac gtc get tgg agt ttg atg ggc gga 864 Leu Asp Gly Lys Lys Ala Gly His Val Ala Trp Ser Leu Met Gly Gly 275 280 285 cac aac cgc atg aac gcg ctc gcc gtc atc gcc gcc gcg cgt cat gcc 912 His Asn Arg Met Asn Ala Leu Ala Val Ile Ala Ala Ala Arg His Ala 290 295 300 gga gtc gac att cag acg gcc tgc gaa gcc ttg age acg ttt aaa aac 960 Gly Val Asp Ile Gin Thr Ala Cys Glu Ala Leu Ser Thr Phe Lys Asn 305 310 315 320 gtc aaa cgc cgc atg gaa atc aaa ggc acg gca aac ggt atc acc gtt 1008 Val Lys Arg Arg Met Glu Ile Lys Gly Thr Ala Asn Gly Ile Thr Val 325 330 335 tac gac gac ttc gcc cac cat ccg acc get atc gaa acc acg att caa 1056 Tyr Asp Asp Phe Ala His His Pro Thr Ala Ile Glu Thr Thr Ile Gin 340 345 350 ggt ttg cgc cag cgc gtc ggc ggc gcg cgc atc ctc gcc gtc ctc gaa 1104 Gly Leu Arg Gin Arg Val Gly Gly Ala Arg Ile Leu Ala Val Leu Glu 355 360 365 ccg cgt tcc aat acg atg aag ctg ggt acg atg aaa gcc gcc ctg ccc 1152 Pro Arg Ser Asn Thr Met Lys Leu Gly Thr Met Lys Ala Ala Leu Pro 370 375 380 gca age ctc aaa gaa gcc gac caa gtg ttc tgc tac gcc ggc ggc gcg 1200 Ala Ser Leu Lys Glu Ala Asp Gin Val Phe Cys Tyr Ala Gly Gly Ala 385 390 395 400 gac tgg gac gtt gcc gaa gcc ctc gcg cct ttg ggc ggc agg ctg cac 1248 Asp Trp Asp Val Ala Glu Ala Leu Ala Pro Leu Gly Gly Arg Leu His 405 410 415 gtc ggc aaa gac ttc gat gcc ttc gtt gcc gaa atc gtg aaa aac gcc 1296 Val Gly Lys Asp Phe Asp Ala Phe Val Ala Glu Ile Val Lys Asn Ala 420 425 430

272 • 4

WO 01/85772

gaa Glu gca ggc gac cat att ttg gtg atg Ala Gly Asp 435 His Ile Leu Val Met 440 ata cac acc aaa ctg ctg gac gct ttg Ile His Thr Lys Leu Leu Asp Ala Leu 450 455

PCT/GBO1/02003 age aac ggc ggt ttc ggc gga 1344 Ser Asn Gly Gly Phe Gly Gly

445 aga 1374

Arg <210> 136 <211> 458 <212> PRT

<213> Neisseria <400> 136 meningitidis Met 1 Lys His Ile His 5 Ile Ile Gly Ile Gly 10 Gly Thr Phe Met Gly 15 Gly Ile Ala Ala Ile 20 Ala Lys Glu Ala Gly 25 Phe Glu Val Ser Gly 30 Cys Asp Ala Lys Met 35 Tyr Pro Pro Met Ser 40 Thr Gin Leu Glu Ala 45 Leu Gly Ile Gly Val 50 Tyr Glu Gly Phe Asp 55 Thr Ala Gin Leu Asp 60 Glu Phe Lys Ala Asp 65 Val Tyr Val Ile Gly 70 Asn Val Ala Lys Arg 75 Gly Met Asp Val Val 80 Glu Ala Ile Leu Asn 85 Arg Gly Leu Pro Tyr 90 Ile Ser Gly Pro Gin 95 Trp Leu Ala Glu Asn 100 Val Leu His His His 105 Trp Val Leu Gly Val 110 Ala Gly Thr His Gly 115 Lys Thr Thr Thr Ala 120 Ser Met Leu Ala Trp 125 Val Leu Glu Tyr Ala 130 Gly Leu Ala Pro _xGly 135 Phe Leu Ile Gly Gly 140 Val Pro Glu Asn Phe 145 Ser Val Ser Ala Arg 150 Leu Pro Gin Thr Pro 155 Arg Gin Asp Pro Asn 160 Ser Gin Ser Pro Phe 165 Phe Val Ile Glu Ala 170 Asp Glu Tyr Asp Thr 175 Ala

273

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Phe Phe Asp Lys 180 Arg Ser Lys Phe Val 185 His Tyr Arg Pro Arg 190 Thr Ala Val Leu Asn 195 Asn Leu Glu Phe Asp 200 His Ala Asp Ile Phe 205 Ala Asp Leu Gly Ala .210 Ile Gin Thr Gin Phe 215 His His Leu Val Arg 220 Thr Val Pro Ser Glu 225 Gly Leu Ile Val Cys 230 Asn Gly Arg Gin Gin 235 Ser Leu Gin Asp Thr 240 Leu Asp Lys Gly Cys 245 Trp Thr Pró Val Glu 250 Lys Phe Gly Thr Glu 255 His Gly Trp Gin Ala 260 Gly Glu Ala Ásn Ala 265 Asp Gly Ser Phe Asp 270 Val Leu Leu Asp Gly 275 Lys Lys Ala Gly His 280 Val Ala Trp Ser Leu 285 Met Gly Gly His Asn 290 Arg Met Asn Ala Leu 295 Ala Val Ile Ala Ala 300 Ala Arg His Ala Gly 305 Val Asp Ile Gin Thr 310 Ala Cys Glu Ala Leu 315 Ser Thr Phe Lys Asn 320 Val Lys Arg Arg Met 325 Glu Ile Lys Gly Thr 330 Ala Asn Gly Ile Thr 335 Val Tyr Asp Asp Phe 340 Ala His His Pro Thr 345 Ala Ile Glu Thr Thr 350 Ile Gin Gly Leu Arg 355 Gin Arg Val Gly Gly 360 Ala Arg Ile Leu Ala 365 Val Leu Glu Pro Arg 370 Ser Asn Thr Met Lys 375 Leu Gly Thr Met Lys 380 Ala Ala Leu Pro Ala 385 Ser Leu Lys Glu Ala 390 'Asp Gin Val Phe Cys 395 Tyr Ala Gly Gly Ala 400 Asp Trp Asp Val Ala 405 Glu Ala Leu Ala Pro 410 Leu Gly Gly Arg Leu 415 His Val Gly Lys Asp 420 Phe Asp Ala Phe Val 425 Ala Glu Ile Val Lys 430 Asn Ala

274

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Glu Ala Gly Asp His Ile Leu Val Met Ser Asn Gly Gly Phe Gly Gly 435 440 445

Ile His Thr Lys Leu Leu Asp Ala Leu Arg 450 455 <210> 137 <211> 876 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(876) <400> 137

ttg Leu 1 acc gtc Thr Val ega acg aaa aag acg gcg cat tat acc cta ttc cat tcc 48 Arg Thr 5 Lys Lys Thr Ala His 10 Tyr Thr Leu Phe His 15 Ser gac ega aaa ccg aac atg act act čte aaa ccc gcc ctg ccc get tat 96 Asp Arg Lys Pro Asn Met Thr Thr Leu Lys Pro Ala Leu Pro Ala Tyr 20 25 30 ctg gac aac atc cgc atc atc ctc acg cgc acc age cat ccc gcc aac 144 Leu Asp Asn Ile Arg Ile Ile Leu Thr Arg Thr Ser His Pro Ala Asn 35 40 45 atc ggc tet gcc gcg cgc gcg atg aaa aca atg ggt ctg cac aaa ctg 192 Ile Gly Ser Ala Ala Arg Ala Met Lys Thr Met Gly Leu His Lys Leu 50 55 60 acc atc gtc gcc cca aat ctg atg gca acg ccg atg acg gaa aac ccg 240 Thr Ile Val Ala Pro Asn Leu Met Ala Thr Pro Met Thr Glu Asn Pro 65 70 75 80 ccc gtg ttt gac ccg gag cat cct caa tcg ttt aaa tta ccg gaa gaa 288 Pro Val Phe Asp Pro Glu -His Pro Gin Ser Phe Lys Leu Pro Glu Glu 85 90 95 age ttc atc ctc get tcc ggc gcg gca gac gtt ttg gaa aat gcc acc 336 Ser Phe Ile Leu Ala Ser Gly Ala Ala Asp Val Leu Glu Asn Ala Thr 100 105 110 att gcc get tet ttg gac gaa gcc ctt gcc gac acc acc atc gcc tgc 384 Ile Ala Ala Ser Leu Asp Glu Ala Leu Ala Asp Thr Thr Ile Ala Cys

275

00 ·· 00 0 ·· 0000 0 0 0 0 «··· 0 0 0 « 0 0 · · 000 0 0 0 00 0 0 0 · 0

0 0 0· 0 000.0

0000 0000 00 000 00 00

WO 01/85772 PCT/GB01/02003

115 120 125 gcc ctg acc agc cgc cgc cgc gaa att act gcg ccg ctg caa acc ccg 432 Ala Leu 130 Thr Ser Arg Arg Arg 135 Glu Ile Thr Ala Pro 140 Leu Gin Thr Pro cgc gat ttg gta tcc gaa tta ctg cag acc gca aac ega ggc gag aaa 480 Arg 145 Asp Leu Val Ser Glu 150 Leu Leu Gin Thr Ala 155 Asn Arg Gly Glu Lys 160 gtg gca ctg gtt ttc ggc aac gag act ttc ggc ttg agc atc gaa gaa 528 Val Ala Leu Val Phe 165 Gly Asn Glu Thr Phe 170 Gly Leu Ser Ile Glu 175 Glu gtc caa gcc tgc aac ega ctg atg acc atc aac ggc aat ccc gac tat 576 Val Gin Ala Cys 180 Asn Arg Leu Met Thr 185 Ile Asn Gly Asn Pro 190 Asp Tyr ttc tcg ctc aac ctc gcc caa gcc gtg cag gtc gtg tgc tac gaa atc 624 Phe Ser Leu 195 Asn Leu Ala Gin Ala 200 Val Gin Val Val Cys 205 Tyr Glu Ile ttc agc caa acc ggt tcg ccc atg acc cat ctt caa caa gaa gac cac 672 Phe Ser 210 Gin Thr Gly Ser Pro 215 Met Thr His Leu Gin 220 Gin Glu Asp His get gcg acc cac gag caa atc aaa ggc atg gtc gcc cac atg gaa agc 720 Ala 225 Ala Thr His Glu Gin 230 Ile Lys Gly Met Val 235 Ala His Met Glu Ser 240 gtg atg aac gac atc ggc ttt ttc aac cgc cgc aac ggc gag cgt ctg 768 Val Met Asn Asp Ile 245 Gly Phe Phe Asn Arg 250 Arg Asn Gly Glu Arg 255 Leu atg cgc cgt atg cag agc ctg ttc gga cgc gcc aac acg caa acc gaa 816 Met Arg Arg Met 260 Gin Sér Leu Phe Gly 265 Arg Ala Asn Thr Gin 270 Thr Glu gac atc gat atc ctg cgc ggt ttt ttc aat acc gtc agc cat cgt atc 864 Asp Ile Asp 275 Ile Leu Arg' -Gly Phe 280 Phe Asn Thr Val Ser 285 His Arg Ile

cat aaa aaa gac 876

His Lys Lys Asp

290 <210> 138

276 ·· ··

I · · « • β ····

WO 01/85772 PCT/GB01/02003 <211> 292 <212> PRT <213> Neisseria meningitidis <400> 138

Leu Thr Val Arg Thr Lys Lys Thr Ala His Tyr Thr Leu Phe His Ser 1 5 10 15 Asp Arg Lys Pro Asn Met Thr Thr Leu Lys Pro Ala Leu Pro Ala Tyr 20 25 30 Leu Asp Asn Ile Arg Ile Ile Leu Thr Arg Thr Ser His Pro Ala Asn 35 40 45 Ile Gly Ser Ala Ala Arg Ala Met Lys Thr Met Gly Leu His Lys Leu 50 55 60 Thr Ile Val Ala Pro Asn Leu Met Ala Thr Pro Met Thr Glu Asn Pro 65 70 75 80 Pro Val Phe Asp Pro Glu His Pro Gin Ser Phe Lys Leu Pro Glu Glu 85 90 95 Ser Phe Ile Leu Ala Ser Gly Ala Ala Asp .Val Leu Glu Asn Ala Thr 100 105 110 Ile Ala Ala Ser Leu Asp Glu Ala Leu Ala Asp Thr Thr Ile Ala Cys 115 120 125 Ala Leu Thr Ser Arg Arg Arg Glu Ile Thr Ala Pro Leu Gin Thr Pro 130 135 140 Arg Asp Leu Val Ser Glu Leu Leu Gin Thr Ala Asn Arg Gly Glu Lys 145 150 155 160 Val Ala Leu Val Phe Gly Asn Glu Thr Phe Gly Leu Ser Ile Glu Glu 165 170 175 Val Gin Ala Cys Asn Arg Leu Met Thr Ile Asn Gly Asn Pro Asp Tyr 180 185 190 Phe Ser Leu Asn Leu Ala Gin Ala Val Gin Val Val Cys Tyr Glu Ile 195 200 205 Phe Ser Gin Thr Gly Ser Pro Met Thr His Leu Gin Gin Glu Asp His 210 215 220 Ala Ala Thr His Glu Gin Ile Lys Gly Met Val Ala His Met Glu Ser

277 ·· ·«·· ·· ·· ·· • » « · · Λ · * · · • · · 0 · ♦ · · · ···· ·♦·· ·« « • V · • · ·

4 4 « • · · ·

0· ··

WO 01/85772 PCT/GB01/02003

225 230 235 240 Val Met Asn Asp Ile 245 Gly Phe Phe Asn Arg 250 Arg Asn Gly Glu Arg 255 Leu Met Arg Arg Met 260 Gin Ser Leu Phe Gly Arg 265 Ala Asn Thr Gin 270 Thr Glu Asp Ile Asp 275 Ile Leu Arg Gly Phe 280 Phe Asn Thr Val Ser 285 His Arg Ile His Lys Lys Asp

290 <210> 139 <211> 708 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(708, <400> 139

ttg cgg cag ctt gga tgt ttg att Leu Ile ggt ttt ttc agt gtt gga atc att 48 Leu 1 Arg Gin Leu Gly 5 Cys Gly Phe 10 Phe Ser Val Gly Ile 15 Ile atg aat ttg aaa tta gtg ttt gaa tcg ggc gat ccc gtc ctg atc ggt 96 Met Asn Leu Lys Leu Val Phe Glu Ser Gly Asp Pro Val Leu Ile Gly 20 25 30 gtg ttt gtg ttg atg ctg ttg atg agt atc gta acg tgg tgt ttg gtt 144 Val Phe Val Leu Met Leu Leu Met Ser Ile Val Thr Trp Cys Leu Val 35 40 45 gtc ttg cgc tgc atc aag ctg tat cgg gcg cgc aaa ggg aat gcc gcc 192 Val Leu Arg Cys Ile Lys -Leu Tyr Arg Ala Arg Lys Gly Asn Ala Ala 50 55 60 gtc aaa cgg cat atg cgc gat act ttg tcg ctg aac gac gcg gtc gaa 240 Val Lys Arg His Met Arg Asp Thr Leu Ser Leu Asn Asp Ala Val Glu 65 70 75 80 aaa gtg cgc gcc gtc gat gcg cct ttg tcc aaa ctg gcg caa gag gca 288 Lys val Arg Ala Val Asp Ala Pro Leu Ser Lys Leu Ala Gin Glu Ala

278

WO 01/85772 PCT/GB»1/<I2OO3

85 90 95 ttg cag tet tac cgc aac tac cgc ega aac gaa gcg tcc gaa ctg gcg 336 Leu Gin Ser Tyr Arg Asn Tyr Arg Arg Asn Glu Ala Ser Glu Leu Ala 100 105 110 cag gct ttg ccg ttg aac gag tat ttg gtc att caa atc cgc aac agt 384 Gin Ala Leu Pro Leu Asn Glu Tyr Leu Val Ile Gin Ile Arg Asn Ser 115 120 125 atg gcg cag att atg cgc cgg ttt gat tac ggg atg acc gcg ctt gcc 432 Met Ala Gin Ile Met Arg Arg Phe Asp Tyr Gly Met Thr Ala Leu Ala 130 135 140 tcc atc ggc gcg acc gcg ccg ttt atc ggg ttg ttc ggc acg gtt tgg 480 Ser Ile Gly Ala Thr Ala Pro Phe Ile Gly Leu Phe Gly Thr Val Trp 145 150 155 160 ggg att tac cac gcc ctg atc aat atc ggg caa agc ggg cag atg agt 528 Gly Ile Tyr His Ala Leu Ile Asn Ile Gly Gin Ser Gly Gin Met Ser 165 170 175 att gcg gcg gtt gcc ggc ccg att ggc gag gca ctg gtg gcg acg gcg 576 Ile Ala Ala Val Ala Gly Pro Ile Gly Glu Ala Leu Val Ala Thr Ala 180 185 190 gcg ggt ttg ttc gtg gcg att ccg gcg gtg ttg gca tac aac ttc ctc 624 Ala Gly Leu Phe Val Ala Ile Pro Ala val Leu Ala Tyr Asn Phe Leu 195 200 205 aat cgc ggc aca aaa ata ctg acc cag gat ttg gat gcg atg gcg cac 672 Asn Arg Gly Thr Lys Ile Leu Thr Gin Asp Leu Asp Ala Met Ala His

210 215 220

gat ttg cac gtc cgc ctg ctt aat caa aag gat agc 708 Asp Leu His Val Arg Leu Leu Asn Gin Lys Asp Ser 225 230 235

<210> 140 <211> 236 <212> PRT <213> Neisseria meningitidis <400> 140

Leu Arg Gin Leu Gly Cys Leu Ile Gly Phe Phe Ser Val Gly Ile Ile 1 5 10 .15

279 • ·

WO 01/85772 PCT/GBO1/02003

Met Asn Leu Lys Leu Val Phe Glu Ser Gly Asp Pro Val Leu Ile Gly 20 25 30 Val Phe Val Leu Met Leu Leu Met Ser Ile Val Thr Trp Cys Leu Val 35 40 45 Val Leu Arg Cys Ile Lys Leu Tyr Arg Ala Arg Lys Gly Asn Ala Ala 50 55 60 Val Lys Arg His Met Arg Asp Thr Leu Ser Leu Asn Asp Ala Val Glu 65 70 75 80 Lys Val Arg Ala Val Asp Ala Pro Leu Ser Lys Leu Ala Gin Glu Ala 85 90 95 Leu Gin Ser Tyr Arg Asn Tyr Arg Arg Asn Glu Ala Ser Glu Leu Ala 100 105 110 Gin Ala Leu Pro Leu Asn Glu Tyr Leu Val Ile Gin Ile Arg Asn Ser 115 120 125 Met Ala Gin Ile Met Arg Arg Phe Asp Tyr Gly Met Thr Ala Leu Ala 130 135 140 Ser Ile Gly Ala Thr Ala Pro Phe Ile Gly Leu Phe Gly Thr Val Trp 145 150 155 160 Gly Ile Tyr His Ala Leu Ile Asn Ile Gly Gin Ser Gly Gin Met Ser 165 170 175 Ile Ala Ala Val Ala Gly Pro Ile Gly Glu Ala Leu Val Ala Thr Ala 180 185 190 Ala Gly Leu Phe Val Ala Ile Pro Ala val Leu Ala Tyr Asn Phe Leu 195 200 205 Asn Arg Gly Thr Lys Ile Leu Thr Gin Asp Leu Asp Ala Met Ala His 210 215 220 Asp Leu His Val Arg Leu' Leu Asn Gin Lys Asp Ser 225 230 235

<210> 141 <211> 812 <212> DNA <213> Neisseria meningitidis

280 i

WO 01/85772 PCT/GBO1/02003 <22 0>

<221> CDS <222> (1)..(810) <400> 141

atg act atg cac His gcc ctc ccc cgc tac gcc gtt ttt ggc aac ccc gtc 48 Met Thr 1 Met Ala 5 Leu Pro Arg Tyr Ala 10 Val Phe Gly Asn Pro 15 Val gcc cac age aaa tcg ccg caa att cat caa caa ttt gcc ctt cag gaa 96 Ala His Ser Lys Ser Pro Gin Ile His Gin Gin Phe Ala Leu Gin Glu 20 25 30 ggc gtt gac att gaa tac gaa cgc att tgc gcc gac atc ggc ggt ttc 144 Gly Val Asp Ile Glu Tyr Glu Arg Ile Cys Ala Asp Ile Gly Gly Phe 35 40 45 gcg cag gcg gtt tcg aca ttt ttt gaa aca ggc ggt tgc ggg gca aac 192 Ala Gin Ala Val Ser Thr Phe Phe Glu Thr Gly Gly Cys Gly Ala Asn 50 55 60 gtt acc gta ccg ttc aag cag gaa gcg ttt cat ctg gcg gac gag cat 240 val Thr Val Pro Phe Lys Gin Glu Ala Phe His Leu Ala Asp Glu His 65 70 75 80 tet gaa cgc gca ttg get gcc ggc gcg gtc aac acg ctg att ttt ctg 283 Ser Glu Arg Ala Leu Ala Ala Gly Ala Val Asn Thr Leu Ile Phe Leu 85 90 95 aaa aac gga aaa ctg cgc ggc gac aat acc gac ggt atc ggt ttg gcc 336 Lys Asn Gly Lys Leu Arg Gly Asp Asn Thr Asp Gly Ile Gly Leu Ala 100 105 110 aac gac atc acg cag gtc aaa aac att gcc atc gaa ggc aaa acc atc 384 Asn Asp Ile Thr Gin Val Lys Asn Ile Ala Ile Glu Gly lys Thr Ile 115 120 125 ttg ctt ttg ggc gcg ggc ggc gcg gtg cgc ggc gtg att cct gtt ttg 432 Leu Leu Leu Gly Ala Gly Gly Ala Val Arg Gly Val Ile Pro Val Leu 130 '135 140 aaa gaa cac cgt cct gcc cgt atc gtc att gcc aac cgt acc cgc gcc 480 Lys Glu His Arg Pro Ala Arg Ile Val Ile Ala Asn Arg Thr Arg Ala 145 150 155 160 aaa gcc gag gaa ttg gcg cag ctt ttc ggc att gaa gcc gtc ccg atg 528 Lys Ala Glu Glu Leu Ala Gin Leu Phe Gly Ile Glu Ala Val Pro Met

281 • · · ·· · · · · • · · · · · • · · · · • ···· · · · · ·

WO 01/85772 PCT/GBO1/02003

165 170 175 gcg gat gtg aac ggc ggt ttt gat atc atc atc aac ggc acg tcg ggc 576 Ala Asp Val Asn Gly Gly Phe Asp Ile Ile Ile Asn Gly Thr Ser Gly 180 185 190 ggt cta aac ggt cag att ccc gat att ccg ccc gat att ttt caa aac 624 Gly Leu Asn Gly Gin Ile Pro Asp Ile Pro Pro Asp Ile Phe Gin Asn 195 200 205 tgc gcg ctt gcc tac gat atg gtg tac ggc tgc gcg gca aaa ccg ttt 672 Cys Ala Leu Ala Tyr Asp Met Val Tyr Gly Cys Ala Ala Lys Pro Phe 210 215 220 tta gat ttt gca ega caa tcg ggt gcg aaa aaa act gcc gac gga ctg 720 Leu Asp Phe Ala Arg Gin Ser Gly Ala Lys Lys Thr Ala Asp Gly Leu 225 230 235 240 ggt atg cta gtc ggt caa gcg gcg gct tcc tac gcc ctc tgg cgc gga 768 Gly Met Leu Val Gly Gin Ala Ala Ala Ser Tyr Ala Leu Trp Arg Gly 245 250 255 ttt acg ccc gat atc cgc ccc gtt atc gaa tac atg aaa gcc ct 812 Phe Thr Pro Asp Ile Arg Pro Val Ile Glu Tyr Met Lys Ala 260 265 270

<210> .142 <211> 270 <212> PRT <213> Neisseria meningitidis <400> 142

Met 1 Thr Met His Ala 5 Leu Pro Arg Tyr Ala 10 Val Phe Gly Asn Pro 15 Val Ala His Ser Lys 20 Ser Pro Gin Ile His 25 Gin Gin Phe Ala Leu 30 Gin Glu Gly Val Asp 35 Ile Glu Tyr- Glu Arg 40 Ile Cys Ala Asp Ile 45 Gly Gly Phe Ala Gin 50 Ala Val Ser Thr Phe 55 Phe Glu Thr Gly Gly 60 Cys Gly Ala Asn Val Thr Val Pro Phe Lys Gin Glu Ala Phe His Leu Ala Asp Glu His

65 70 75 80

282

WO 01/85772

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Ser Glu Arg Ala Leu Ala Ala Gly Ala Val Asn Thr Leu Ile Phe Leu 85 90 95 Lys Asn Gly Lys Leu Arg Gly Asp Asn Thr Asp Gly Ile Gly Leu Ala 100 105 110 Asn Asp Ile Thr Gin Val Lys Asn Ile Ala Ile Glu Gly Lys Thr Ile 115 120 125 Leu Leu Leu Gly Ala Gly Gly Ala Val Arg Gly Val Ile Pro Val Leu 130 135 140 Lys Glu His Arg Pro Ala Arg Ile Val Ile Ala Asn Arg Thr Arg Ala 145 150 155 160 Lys Ala Glu Glu Leu Ala Gin Leu Phe Gly Ile Glu Ala Val Pro Met 165 170 175 Ala Asp Val Asn Gly Gly Phe Asp Ile Ile Ile Asn Gly Thr Ser Gly 180 185 190 Gly Leu Asn Gly Gin Ile Pro Asp Ile Pro Pro Asp Ile Phe Gin Asn 195 200 205 Cys Ala Leu Ala Tyr Asp Met Val Tyr Gly Cys Ala Ala Lys Pro Phe 210 215 220 Leu Asp Phe Ala Arg Gin Ser Gly Ala Lys Lys Thr Ala Asp Gly Leu 225 230 235 240 Gly Met Leu Val Gly Gin Ala Ala Ala Ser Tyr Ala Leu Trp Arg Gly 245 250 255 Phe Thr Pro Asp Ile Arg Pro Val Ile Glu Tyr Met Lys Ala

260 265 270 <210> 143 <211> 1515 <212> DNA ' <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1515) <400> 143

283

WO 01/85772

PCT/GBO1/02003

atg ttg tat ttc aga tac ggt ttt ttg gtt gtt tgg tgt gcg gca ggt Gly 48 Met Leu Tyr Phe Arg Tyr Gly Phe Leu Val 10 Val Trp Cys Ala Ala 15 1 5 gtt tet gcc gcc tat ggg gcg gat gcg ccc gcg att ttg gat gaa aag 96 Val Ser Ala Ala Tyr Gly Ala Asp Ala Pro Ala Ile Leu Asp Asp Lys 20 25 30 gca ttg ttg cag gtg cag cgg tcg gtg tcg gat aag tgg gcg gaa tcg 144 Ala Leu Leu Gin Val Gin Arg Ser Val Ser Asp Lys Trp Ala Glu Ser 35 40 45 gat tgg aaa gtt gac aat gat gcc ccg cgc gtg gtt gac ggg gat ttt 192 Asp Trp Lys Val Asp Asn Asp Ala Pro Arg Val Val Asp Gly Asp Phe 50 55 60 ttg ttg gcg cat ccg aaa atg ttg gaa cat agt ttg cgc gac gtg ctc 240 Leu Leu Ala His Pro Lys Met Leu Glu His Ser Leu Arg Asp Val Leu 65 70 75 80 aac ggc aat cag gcg gat ttg atc gct tcg ttg gcg gat ttg tat gcc 288 Asn Gly Asn Gin Ala Asp Leu Ile Ala Ser Leu Ala Asp Leu Tyr Ala 85 90 95 aag ctg ccg gat tat gac gcg gtt ttg tac ggc agg gcg cgg gct ttg 336 Lys Leu Pro Asp Tyr Asp Ala Val Leu Tyr Gly Arg Ala Arg Ala Leu 100 105 110 ctg gcg aaa ttg gcg gga agg ccg gcg gag gcg gtg gcg cgg tat cgg 384 Leu Ala Lys Leu Ala Gly Arg Pro Ala GlU Ala Val Ala Arg Tyr Arg 115 120 125 gaa ctg cac ggg gaa aat gcg gca gac gag cgg att ttg ctg gat ttg 432 Glu Leu His Gly Glu Asn Ala Ala Asp Glu Arg Ile Leu Leu Asp Leu 130 135 140 gcg gcg gcg gag ttt gac gat ttc cgg ctg aag tcg gca gaa agg cat 480 Ala Ala Ala Glu Phe Asp Asp Phe Arg Leu Lys Ser Ala Glu Arg His 145 150 155 160 ttt gcc gag gcg gaa aaa ttg gat ttg ccg gcg ccg gtt ttg gaa aat 528 Phe Ala Glu Ala Glu Lys Leu Asp Leu Pro Ala Pro Val Leu Glu Asn 165 170 175 gtg ggg cgt ttt cgg aaa aaa gcg gag ggg ctg acg ggc tgg cgt ttt 576 Val Gly Arg Phe Arg Lys Lys Ala Glu Gly Leu Thr Gly Trp Arg Phe 180 185 190

284

WO 01/85772

PCT/GB01/02003

tcg ggc ggc atc agt ccg gcg gtc aat aga aat gcc aat aat gcc gcg 624 Ser Gly Gly Ile Ser Pro Ala Val Asn Arg Asn Ala Asn Asn Ala Ala 195 200 205 ccg cag tat tgc cgg caa aac gga ggc cgg cag ata tgc agt gtc age 672 Pro Gin Tyr Cys Arg Gin Asn Gly Gly Arg Gin Ile Cys Ser Val Ser 210 215 220 cgg gcg gag cgg gcg gca ggc ttg aat tat gaa atc gag gcg gaa aaa 720 Arg Ala Glu Arg Ala Ala Gly Leu Asn Tyr Glu Ile Glu Ala Glu Lys 225 230 235 240 ctg acg gcg ttg gca gat aat cat tat ttg ttg ttc cgt tcc aat atc 768 Leu Thr Ala Leu Ala Asp Asn His Tyr Leu Leu Phe Arg Ser Asn Ile 245 250 255 ggc ggc acg age tat tat ttc agt aaa aaa tea get tat gac gac ggg 816 Gly Gly Thr Ser Tyr Tyr Phe Ser Lys Lys Ser Ala Tyr Asp Asp Gly 260 265 270 ttc ggc aga gcg tat ttg ggt tgg cag tat aaa aat gca cgg cag acg 864 Phe Gly Arg Ala Tyr Leu Gly Trp Gin Tyr Lys Asn Ala Arg Gin Thr 275 280 285 gcg ggg att ttg ccg ttt tat cag gtg cag ttg tcg ggc age gac ggc 912 Ala Gly Ile Leu Pro Phe Tyr Gin Val Gin Leu Ser Gly Ser Asp Gly 290 295 300 ttt gat gcg aaa aca aaa cgg gta aac aac cgc cgc ctg ccg ccg tat 960 Phe Asp Ala Lys Thr Lys Arg Val Asn Asn Arg Arg Leu Pro Pro Tyr 305 310 315 320 atg ctg gcg cac gga gtc ggc gtg cag ttg tcc cat act tac cgc cca 1008 Met Leu Ala His Gly Val Gly Val Gin Leu Ser His Thr Tyr Arg Pro 325 330 335 aac ccg gga tgg caa ttt tcg gtc gcg ctg gaa cat tac cgc caa cgc 1056 Asn Pro Gly Trp Gin Phe Ser Val Ala Leu Glu His Tyr Arg Gin Arg 340 345 350 tac cgc gaa cag gat agg «gcg gaa tac aat aac ggt cgg cag gac ggg 1104 Tyr Arg Glu Gin Asp Arg Ala Glu Tyr Asn Asn Gly Arg Gin Asp Gly 355 360 365 ttt tat gtt tcg tcg gca aaa cgt ttg ggc gaa tcg gca act gtg ttc 1152 Phe Tyr Val Ser Ser Ala Lys Arg Leu Gly Glu ser Ala Thr Val Phe

370

375

380

285

WO 01/85772 PCT/GB01/02003

ggc Gly 385 ggc tgg cag ttt gtg cgg ttt gtg ccg aaa cgc gaa acg gtg ggc 1200 Gly Trp Gin Phe Val 390 Arg Phé Val Pro Lys 395 Arg Glu Thr Val Gly 400 ggc gcg gtc aat aat gcc gcc tac cgg cgc aac ggt gtt tat gcc ggc 1248 Gly Ala Val Asn Asn Ala Ala Tyr Arg Arg Asn Gly Val Tyr Ala Gly 405 410 415 tgg gcg cag gag tgg cgg cag ttg ggc ggt ttg aac agt cgg gtt tcc 1296 Trp Ala Gin Glu Trp Arg Gin Leu Gly Gly Leu Asn Ser Arg Val Ser 420 425 430 gcg tet tat gcc cgc cgc aac tat aag ggc gtt gcg gct ttc tcg aca 1344 Ala Ser Tyr Ala Arg Arg Asn Tyr Lys Gly Val Ala Ala Phe Ser Thr 435 440 445 gag gcg caa cgc aac cgc gaa tgg aat gtc tcg ctg gct ttg age cac 1392 Glu Ala Gin Arg Asn Arg Glu Trp Asn Val Ser Leu Ala Leu Ser His 450 455 460 gac aag ttg tcg tac aaa ggt atc gtg ccc gcg ttg aat tat cgt ttc 1440 Asp Lys Leu Ser Tyr Lys Gly Ile Val Pro Ala Leu Asn Tyr Arg Phe 465 470 475 480 ggc agg acg gaa agt aat gtg ccg tat gcg aaa cgc cgc aac age gag 1488 Gly Arg Thr Glu Ser Asn Val Pro Tyr Ala Lys Arg Arg Asn Ser Glu 485 490 495 gtg ttt gtg tcg gcg gat tgg cgg ttt 1515 Val Phe Val Ser Ala Asp Trp Arg Phe 500 505

<210> 144 <211> 505 <212> PRT <213> Neisseria meningitidis <400> 144

Met Leu Tyr Phe 1 Arg Tyr 5 Gly Phe Leu Val Val Trp Cys Ala Ala Gly 10 15 Val Ser Ala Ala Tyr Gly Ala Asp Ala Pro Ala Ile Leu Asp Asp Lys 20 25 30 Ala Leu Leu Gin Val Gin Arg Ser Val Ser Asp Lys Trp Ala Glu Ser

35 40 45

286

WO 01/85772 PCT/GB01/02003 Asp Trp Lys Val Asp Asn 50 Asp Ala Pro Arg Val Val. Asp Gly Asp Phe 55 60 Leu Leu Ala His Pro Lys 65 70 Met Leu Glu His Ser Leu Arg Asp Val Leu 75 80 Asn Gly Asn Gin Ala Asp 85 Leu Ile Ala Ser Leu Ala Asp Leu Tyr Ala 90 95 Lys Leu Pro Asp Tyr Asp 100 Ala Val Leu Tyr Gly Arg Ala Arg Ala Leu 105 110 Leu Ala Lys Leu Ala Gly Arg Pro Ala Glu Ala Val Ala Arg Tyr Arg 115 120 125 Glu Leu His Gly Glu Asn 130 Ala Ala Asp Glu Arg Ile Leu Leu Asp Leu 135 140 Ala Ala Ala Glu Phe Asp 145 150 Asp Phe Arg Leu Lys Ser Ala Glu Arg His 155 160 Phe Ala Glu Ala Glu Lys 165 Leu Asp Leu Pro Ala Pro Val Leu Glu Asn 170 175 Val Gly Arg Phe Arg Lys 180 Lys Ala Glu Gly Leu Thr Gly Trp Arg Phe 185 190 Ser Gly Gly Xle Ser Pro 195 Ala Val Asn Arg Asn Ala Asn Asn Ala Ala 200 205 Pro Gin Tyr Cys Arg Gin 210 Asn Gly Gly Arg Gin Ile Cys Ser Val Ser 215 220 Arg Ala Glu Arg Ala Ala 225 230 Gly Leu Asn Tyr Glu Ile Glu Ala Glu Lys 235 240 Leu Thr Ala Leu Ala Asp 245 Asn His Tyr Leu Leu Phe Arg Ser Asn Ile 250 255 Gly Gly Thr Ser Tyr Tyr 260 Phe Ser Lys Lys Ser Ala Tyr Asp Asp Gly 265 270 Phe Gly Arg Ala Tyr Leu 275 Gly Trp Gin Tyr Lys Asn Ala Arg Gin Thr 280 285 Ala Gly Ile Leu Pro Phe 290 Tyr Gin Val Gin Leu Ser Gly Ser Asp Gly 295 300

287

• 9 ·· 9« 9 • 9 9 9 9 • · · 9 • · 9 9 9 9 • 9 • · 9 · • • 9 9 9 9 9 9 9 • 999 9999 99 9 9 9 99 9 9

WO 01/85772 PCT/GB01/02003 Phe Asp Ala Lys Thr Lys Arg Val Asn Asn Arg Arg Leu Pro 305 310 315 Pro Tyr 320 Met Leu Ala His Gly Val Gly Val Gin Leu Ser His Thr Tyr 325 330 Arg Pro 335 Asn Pro Gly Trp Gin Phe Ser Val Ala Leu Glu His Tyr Arg 340 345 350 Gin Arg Tyr Arg Glu Gin Asp Arg Ala Glu Tyr Asn Asn Gly Arg Gin 355 360 365 Asp Gly Phe Tyr Val Ser Ser Ala Lys Arg Leu Gly Glu Ser Ala Thr 370 375 380 Val Phe Gly Gly Trp Gin Phe Val Arg Phe Val Pro Lys Arg Glu Thr 385 390 395 Val Gly 400 Gly Ala Val Asn Asn Ala Ala Tyr Arg Arg Asn Gly Val Tyr 405 410 Ala Gly 415 Trp Ala Gin Glu Trp Arg Gin Leu Gly Gly Leu Asn Ser Arg 420 425 430 Val Ser Ala Ser Tyr Ala Arg Arg Asn Tyr Lys Gly Val Ala Ala Phe 435 440 445 Ser Thr Glu Ala Gin Arg Asn Arg Glu Trp Asn Val Ser Leu Ala Leu 450 455 460 Ser His Asp Lys Leu Ser Tyr Lys Gly Ile Val Pro Ala Leu Asn Tyr 465 470 475 Arg Phe 480 Gly Arg Thr Glu Ser Asn Val Pro Tyr Ala Lys Arg Arg Asn 485 490 Val Phe Val Ser Ala Asp Trp Arg Phe Ser Glu 495

500 505 <210> 145 <211> 840 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS

288 ·· ·»«· «« ·· * • · · · · β ·· • · · · · • · · · · · • · · · · ········ ·· ··Λ • · · · ·· ··

WO 01/85772 PCT/GB01/02003 <222> (1)..(840) <400> 145

atg gat aaa gaa ega att tta acc ccc gca gtc gtg ttt tcc gtc gca 48 Met 1 Asp Lys Glu Arg 5 Ile Leu Thr Pro Ala 10 Val Val Phe Ser Val 15 Ala ctg ctg cat ttg gca atg gtg gca ttg ctc tgg cag gcg cac aag ctg 96 Leu Leu His Leu Ala 20 Met Val Ala Leu Leu 25 Trp Gin Ala His 30 Lys Leu ccc gtg ata gag tca ggc aat gtt att gaa ttt gtc gat ttg ggc gat 144 Pro Val Ile 35 Glu Ser Gly Asn Val Ile Glu 40 Phe Val Asp 45 Leu Gly Asp ttt ggc gga ggg gac ggc gca ccc gaa ggt gca ggc gcg cct gcc gcg 192 Phe Gly 50 Gly Gly Asp Gly Ala 55 Pro Glu Gly Ala Gly 60 Ala Pro Ala Ala ccc gaa ccg caa ccc gtg ccc gag ccg ccc aaa cct gtc gag ccg ccc 240 Pro 65 Glu Pro Gin Pro Val Pro 70 Glu Pro Pro Lys 75 Pro Val Glu Pro Pro 30 aag ccg gtt ttg aag ccg gtg gtt acg aaa aag gcg gat gcg gat att 288 Lys Pro Val Leu Lys 85 Pro Val Val Thr Lys 90 Lys Ala Asp Ala Asp 95 Ile cag cag cct aag gaa gag ccg aaa cct gaa gaa aag ccg aaa ccc gaa 336 Gin Gin Pro Lys Glu 100 Glu Pro Lys Pro Glu 105 Glu Lys Pro Lys 110 Pro Glu gaa aaa ccg aaa cca gag cct aag ccg gaa gcg aag cct gtc ccg aaa 384 Glu Lys Pro 115 Lys Pro Glu Pro Lys Pro Glu 120 Ala Lys Pro 125 Val Pro Lys ccg gcg gaa aaa ccg gtc gag aag ccg tet gaa aaa cct gcc gaa cat 432 Pro Ala 130 Glu Lys Pro Val Glu 135 Lys Pro Ser Glu Lys 140 Pro Ala Glu His ccc ggc aat gct tet gcc aaa gca gac agc gag cag ggc aat ggg gaa 480 Pro 145 Gly Ásn Ala Ser Ala Lys 150 Ala Asp Ser Glu 155 Gin Gly Asn Gly Glu 160 gat aag gga acc ggt atc aaa gga gac gga acg ggg cgc gga gaa ggc 528 Asp Lys Gly Thr Gly 165 Ile Lys Gly Asp Gly 170 Thr Gly Arg Gly Glu 175 Gly agc ggt aaa ggt agc ggc ggt gtc aaa ggc gaa cac ggg gaa gga gcc 576

289

WO 01/85772

PCT/GB01/02003

Ser Gly Lys Gly 180 Ser Gly Gly Val Lys Gly Glu His Gly Glu Gly Ala 185 190 ggc agc agc aaa ggc aat cct tta cgc gcc aac ggc agc att ccg cgc Gly Ser Ser Lys Gly Asn Pro Leu Arg Ala Asn Gly Ser Ile Pro Arg 195 200 205 ccg gct tat ccc acg ctt tet atg gag aat gat gag cag ggt acg gtt Pro Ala Tyr Pro Thr Leu Ser Met Glu Asn Asp Glu Gin Gly Thr Val 210 215 220 gtt ttg agc gtt ttg gtt tet ccg ggc ggt cat gtt gag tcc gtt aaa val Leu Ser val Leu Val Ser Pro Gly Gly His Val Glu Ser Val Lys 225 230 235 240 atc gtg aaa agc agt ggt ttt tqc cgg ttg gac aat gcg gca cgc aag Ile Val Lys Ser Ser Gly Phe Šer Arg Leu Asp Asn Ala Ala Arg Lys 245 250 2S5 gcg gcg caa aac ggg cat ttt caa gcc aat gcc tgg acg gag ttt aaa Ala Ala Gin Asn Gly His Phe Gin Ala Asn Ala Trp Thr Glu Phe Lys 260 265 270 gtc ccc gtc aag ttt gaa ttg aat Val Pro Val Lys Phe Glu Leu Asn 275 280

672

720

768

816

840

624 <210> 146 <211> 280 <212> PRT

<213> Neisseria meningitidis <400> 146 Met Asp Lys Glu Arg Ile Leu Thr Pro Ala Val Val Phe Ser Val Ala 1 5 10 15 Leu Leu His Leu Ala Met Val Ala Leu Leu Trp Gin Ala His Lys Leu 20 25 30 Pro Val Ile Glu Ser Gly Asn Val Ile Glu Phe Val Asp Leu Gly Asp 35 40 45 Phe Gly Gly Gly Asp Gly Ala Pro Glu Gly Ala Gly Ala Pro Ala Ala 50 55 60 Pro Glu Pro Gin Pro Val Pro Glu Pro Pro Lys Pro Val Glu Pro Pro 65 70 75 80

290

9 · ·· ·· • • · • · · • • · · • • · • · 9 • • 9 • • ♦ 9 9 • 9 9 · • fr 9 • • • fr ··· · ··· • • · 99 9 ·.· • ·

WO 01/85772 PCT/GB01/02003

Lys Pro Val Leu Lys Pro Val Val Thr Lys Lys Ala Asp Ala Asp Ile 85 90 95 Gin Gin Pro Lys Glu Glu Pro Lys Pro Glu Glu Lys Pro Lys Pro Glu 100 105 110 Glu Lys Pro Lys Pro Glu Pro Lys Pro Glu Ala Lys Pro Val Pro Lys 115 120 125 Pro Ala Glu Lys Pro Val Glu Lys Pro Ser Glu Lys Pro Ala Glu His 130 135 140 Pro Gly Asn Ala Ser Ala Lys Ala Asp Ser Glu Gin Gly Asn Gly Glu 145 150 155 160 Asp Lys Gly Thr Gly Ile Lys Gly Asp Gly Thr Gly Arg Gly Glu Gly 165 170 175 Ser Gly Lys Gly Ser Gly Gly Val Lys Gly Glu His Gly Glu Gly Ala 180 185 190 Gly Ser Ser Lys Gly Asn Pro Leu Arg Ala Asn Gly Ser Ile Pro Arg 195 200 205 Pro Ala Tyr Pro Thr Leu Ser Met Glu Asn Asp Glu Gin Gly Thr Val 210 215 220 Val Leu Ser Val Leu Val Ser Pro Gly Gly His Val Glu Ser Val Lys 225 230 235 240 Ile Val Lys Ser Ser Gly Phe Ser Arg Leu Asp Asn Ala Ala Arg Lys 245 250 255 Ala Ala Gin Asn Gly His Phe Gin Ala Asn Ala Trp Thr Glu Phe Lys 260 265 270 Val Pro Val Lys Phe Glu Leu Asn

275 280 <210> 147 <211> 1572 <212> DNA <213> Neisseria meningitidis <220>

291

WO 01/85772 PCT/GB01/02003 <221> CDS <222> (1)..(1572) <400> 147

atg ttc aga cgg Arg tat ctt ccg aac aga cag atg aat atg gtt tcc aaa 48 Met Phe 1 Arg Tyr 5 Leu Pro Asn Arg Gin 10 Met Asn Met Val Ser 15 Lys ctg gac aaa tac tgg cag cac ccc gcc ctc tac tgg cct ttg ctc atc 96 Leu Asp Lys Tyr Trp Gin His Pro Ala Leu Tyr Trp Pro Leu Leu Ile 20 25 30 ctt ttt gcc gcc gcc acc ccc ttt acc ttc gca ccc tac tac cac ttt 144 Leu Phe Ala Ala Ala Thr Pro Phe Thr Phe Ala Pro Tyr Tyr His Phe ³⁵ 40 45 tgg ctg atg ccc ttg att ttc ggt gcc ttc gtc cgc ctc atc gaa Ctg 192 Trp Leu Met Pro Leu Ile Phe Gly Ala Phe Val Arg Leu Ile Glu Leu 50 55 60 cgt ccg cgt ttt get gtc tet tcc gcc tac ctg ttc ggc ctg acc gca 240 Arg Pro Arg Phe Ala Val Ser Ser Ala Tyr Leu Phe Gly Leu Thr Ala 65 70 75 80 tac acg aca cag ttc tac tgg ata cac acc gcc ctg cac gac gtt tcc 288 Tyr Thr Thr Gin Phe Tyr Trp Ile His Thr Ala Leu His Asp Val Ser 85 90 95 ggc ctg ccc gac ctc tat gcc gta ccg ctg acc ttc cta ctc ccc gcc 336 Gly Leu Pro Asp Leu Tyr Ala Val Pro Leu Thr Phe Leu Leu Pro Ala 100 105 110 tac ctt gcc ctt tat ccg gca ctg tgt ttc tgg ctg tgg aaa aaa ttt 384 Tyr Leu Ala Leu Tyr Pro Ala Leu cys Phe Trp Leu Trp Lys Lys Phe 115 120 125 acc ctg cct cgg ggc ata aaa atc ggt ttg gta ctg ccc atc ctg tgg 432 Thr Leu Pro Arg Gly Ile Lys Ile Gly Leu Val Leu Pro Ile Leu Trp 130 135 140 aca ctg acc gag ttt gcc cgc gaa cgt ttc ctg acc gga ttc ggc tgg 480 Thr Leu Thr Glu Phe Ala Arg Glu Arg Phe Leu Thr Gly Phe Gly Trp 145 150 155 160 ggc gca atc ggc tac tcc caa atc acc ccg gac agc ccg ctc gcc ggc 528 Gly Ala Ile Gly Tyr Ser Gin Ile Thr Pro Asp Ser Pro Leu Ala Gly

165 170 175

292 • · · · · · · · · ···· ···# ·> c·· »· ··

WO 01/85772 PCT/GB01/02003

ttt gcc cca ttt ggc ggc atc cac atg gtt aca ctg gca acc gcc ttt 576 Phe Ala Pro Phe 180 Gly Gly Ile His Met Val 185 Thr Leu Ala Thr 190 Ala Phe ctc ggt gtc tgg ctg gtt ttg gcg agt gat aac acc gca cgt tcg ggc 624 Leu Gly Val Trp Leu Val Leu Ala Ser Asp Asn Thr Ala Arg Ser Gly 195 200 205 aaa cgc ctg ctt cca att atc ctg att gcc gcc ctg ctt gcc gca ggc 672 Lys Arg Leu Leu Pro Ile Ile Leu Ile Ala Ala Leu Leu Ala Ala Gly 210 215 220 tac acc gcc ega caa acc gac ttc acc cgc ccc gac ggc agc cgc agc 720 Tyr Thr Ala Arg Gin Thr Asp Phe Thr Arg Pro Asp Gly Ser Arg Ser 225 230 235 240 acc gtc gcc ctg ctt caa ggc aac atc gac caa acc ctc aaa tgg cgt < 768 Thr Val Ala Leu Leu Gin Gly Asn Ile Asp Gin Thr Leu Lys Trp Arg 245 250 255 gaa gac caa gtc atc ccg acc ata cag aaa tat tac gaa cag gtc ggc 816 Glu Asp Gin Val Ile Pro Thr Ile Gin Lys Tyr Tyr Glu Gin Val Gly 260 265 270 aaa acc act gcc gac atc gtc atc ctg ccc gaa acc gcc atc ccc gtc 864 Lys Thr Thr Ala Asp Ile Val Ile Leu Pro Glu Thr Ala Ile Pro Val 275 280 285 atg cgc caa aac ctg ccg gaa aac ata ctg gca aaa ttt gcc gaa cag 912 Met Arg Gin Asn Leu Pro Glu Asn Ile Leu Ala Lys Phe Ala Glu Gin 290 295 300 gcg caa aac aac ggc agc gcg ctc gcc gtc ggc atc agc caa tac act 960 Ala Gin Asn Asn Gly Ser Ala Leu Ala Val Gly Ile Ser Gin Tyr Thr 305 310 315 320 tcg gac ggc aac ggt tac gaa aac gcc gtc atc aac ctg acc ggt tat 1008 Ser Asp Gly Asn Gly Tyr Glu Asn Ala Val Ile Asn Leu Thr Gly Tyr 325 330 335 cag gaa aac aat cag gac ggc atc ccc tac tac gcc aaa aac cac ctc 1056 Gin Glu Asn Asn Gin Asp Gly Ile Pro Tyr Tyr Ala Lys Asn His Leu

340 345 350

gtc CCC ttc ggc gaa tac aaa ccg ctg CCC ttc ctg acc acg ccg ctt 1104 Val Pro Phe Gly Glu Tyr Lys Pro Leu Pro Phe Leu Thr Thr Pro Leu 355 360 365

293

WO 01/85772 PCT/GBO1/02003

tac aaa atg atg aat atg ccc ctt tcc gac ttc cgc aaa ggc ggc ggt Asn Met Pro Leu Ser Asp Phe Arg Lys Gly Gly Gly 1152 Tyr Lys Met Met 370 375 380 aag caa tcc gcc ctg ctg atg aaa aac caa aaa atc gcc ttc aac atc 1200 Lys Gin Ser Ala Leu Leu Met Lys Asn Gin Lys Ile Ala Phe Asn Ile 385 390 395 400 tgt tac gaa gac gga ttc ggc gac gaa ctg att gcc gcc gcc aaa gat 1248 Cys Tyr Glu Asp Gly Phe Gly Asp Glu Leu Ile Ala Ala Ala Lys Asp 405 410 415 gcc aca ctg ctt gcc aat gcc age aat atg gcg tgg tac gga aaa tcc 1296 Ala Thr Leu Leu Ala Asn Ala Ser Asn Met Ala Trp Tyr Gly Lys Ser 420 425 430 aac gcc atg tac cag cac ctc caa caa tcg cag gcg cgg gct atg gaa 1344 Asn Ala Met Tyr Gin His Leu Gin Gin Ser Gin Ala Arg Ala Met Glu 435 440 445 ctc gga cgc tat atg gtc cgt gcc acc aac acc ggc gca acc gcc atc 1392 Leu Gly Arg Tyr Met Val Arg Ala Thr Asn Thr Gly Ala Thr Ala Ile 450 455 460 atc tcc ccc aaa ggc aac atc atc gcc caa gcc caa ccc gat acg gaa 1440 Xle Ser Pro Lys Gly Asn Ile Ile Ala Gin Ala Gin Pro Asp Thr Glu 465 470 475 480 acc gta ttg gaa gga cac atc aaa ggc tat gtc ggc gaa acg ccc tat 1488 Thr Val Leu Glu Gly His Ile Lys Gly Tyr Val Gly Glu Thr Pro- Tyr 485 490 495 atg aaa acc ggc agt tca tgg tgg ttg atg ggc ata ttg acc cta gcc 1536 Met Lys Thr Gly Ser Ser Trp Trp Leu Met Gly Ile Leu Thr Leu Ala 500 505 510 gca ctg att ctt ttc atc ttc ega aac aaa gaa cac 1572 Ala Leu Ile Leu Phe Ile Phe Arg Asn Lys Glu His 515 520

<210> 148 <211> 524 <212> PRT <213> Neisseria meningitidis <400> 148

Met Phe Arg Arg Tyr Leu Pro Asn Arg Gin Met Asn Met Val Ser Lys

294

WO 01/85772

1 5

PCT/GB01/02003

10 15

Leu Asp Lys Tyr 20 Trp Gin His Pro Ala 25 Leu Tyr Trp Pro Leu 30 Leu Ile Leu Phe Ala 35 Ala Ala Thr Pro Phe 40 Thr Phe Ala Pro Tyr 45 Tyr His Phe Trp Leu 50 Met Pro Leu Ile Phe 55 Gly Ala Phe Val Arg 60 Leu Ile Glu Leu Arg 65 Pro Arg Phe Ala Val 70 Ser Ser Ala Tyr Leu 75 Phe Gly Leu Thr Ala 80 Tyr Thr Thr Gin Phe 85 Tyr Trp Ile His Thr 90 Ala Leu His Asp Val 95 Ser Gly Leu Pro Asp 100 Leu Tyr Ala Val Pro 105 Leu Thr Phe Leu Leu 110 Pro Ala Tyr Leu Ala 115 Leu Tyr Pro Ala Leu 120 Cys Phe Trp Leu Trp 125 Lys Lys Phe Thr Leu 130 Pro Arg Gly Ile Lys 135 Ile Gly Leu Val Leu 140 Pro Ile Leu Trp Thr 145 Leu Thr Glu Phe Ala 150 Arg Glu Arg Phe Leu 155 Thr Gly Phe Gly Trp 160 Gly Ala Xle Gly Tyr 165 Ser Gin Ile Thr Pro 170 Asp Ser Pro Leu Ala 175 Gly Phe Ala Pro Phe 180 Gly Gly Ile His Met 185 Val Thr Leu Ala Thr 190 Ala Phe Leu Gly Val 195 Trp Leu Val Leu Ala 200 Ser Asp Asn Thr Ala 205 Arg Ser Gly Lys Arg 210 Leu Leu Pro Ile Ile 215 Leu Ile Ala Ala Leu 220 Leu Ala Ala Gly Tyr 225 Thr Ala Arg Gin Thr 230 Asp Phe Thr Arg Pro 235 Asp Gly Ser Arg Ser 240 Thr Val Ala Leu Leu 245 Gin Gly Asn Ile Asp 250 Gin Thr Leu Lys Trp 255 Arg Glu Asp Gin Val Ile Pro Thr Ile Gin Lys Tyr Tyr Glu Gin Val Gly

295

WO 01/85772

PCT/GB01/02003

260 265 270

Lys Thr Thr 275 Ala Asp Ile Val Ile Leu Pro Glu Thr Ala Ile Pro Val 280 285 Met Arg Gin Asn Leu Pro Glu Asn Ile Leu Ala Lys Phe Ala Glu Gin 290 295 300 Ala Gin Asn Asn Gly Ser Ala Leu Ala Val Gly Ile Ser Gin Tyr Thr 305 310 315 320 Ser Asp Gly Asn Gly Tyr Glu Asn Ala Val Ile Asn Leu Thr Gly Tyr 325 330 • 335 Gin Glu Asn Asn Gin Asp Gly Ile Pro Tyr Tyr Ala Lys Asn His Leu 340 345 350 Val Pro Phe Gly Glu Tyr Lys Pro Leu Pro Phe Leu Thr Thr Pro Leu 355 360 365 Tyr Lys Met Met Asn Met Pro Leu Ser Asp Phe Arg Lys Gly Gly Gly 370 375 380 Lys Gin Ser Ala Leu Leu Met Lys Asn Gin Lys Ile Ala Phe Asn Ile 385 390 395 400 Cys Tyr Glu Asp Gly Phe Gly Asp Glu Leu Ile Ala Ala Ala Lys Asp 405 410 415 Ala Thr Leu Leu Ala Asn Ala Ser Asn Met Ala Trp Tyr Gly Lys Ser 420 425 430 Asn Ala Met Tyr Gin His Leu Gin Gin Ser Gin Ala Arg Ala Met Glu 435 440 445 Leu Gly Arg Tyr Met Val Arg Ala Thr Asn Thr Gly Ala Thr Ala Ile 450 455 460 Ile Ser Pro Lys Gly Asn Ile Ile Ala Gin Ala Gin Pro Asp Thr Glu 465 470. 475 480 Thr Val Leu Glu Gly His Ile Lys Gly Tyr Val Gly Glu Thr Pro Tyr 485 490 495 Met Lys Thr Gly Ser Ser Trp Trp Leu Met Gly Ile Leu Thr Leu Ala 500 505 510 Ala Leu Ile Leu Phe Ile Phe Arg Asn Lys Glu His

296

WO 01/85772 PCT/GB01/02003

515 520 <210> 149 <211> 558 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1),.(558) <400> 149

ttg Leu 1 gat ctc tac aaa ctc ggt tac aaa caa cat acc cgt acc gat gcg 48 Asp Leu Tyr Lys Leu 5 Gly Tyr Lys Gin 10 His Thr Arg Thr Asp 15 Ala gaa ggc tat atc gag aaa ctg cac att acc ccc gcc aat gcc cat gag 96 Glu Gly Tyr Ile Glu Lys Leu His Ile Thr Pro Ala Asn Ala His Glu 20 25 30 tgc aaa cac ctg ccg ccg ttg ttg gaa gga ctg ccc aaa ggt acg acc 144 čys Lys His Leu Pro Pro Leu Leu Glu Gly Leu Pro Lys Gly Thr Thr 35 40 45 gtc tat gcc gac aaa ggc tac gac agt gcg gaa aac cgg caa cat ctg 192 Val Tyr Ala Asp Lys Gly Tyr Asp Ser Ala Glu Asn Arg Gin His Leu 50 55 60 gaa gaa cat cag ttg ttg gac ggc att atg cgc aaa gcc tgc cgc aac 240 Glu Glu His Gin Leu Leu Asp Gly Ile Met Arg Lys Ala Cys Arg Asn 65 70 75 80 cgt ccg ctg tcg gaa acg caa acc aaa cgc aac cgg tat ttg tcg aag 288 Arg Pro Leu Ser Glu Thr Gin Thr Lys Arg Asn Arg Tyr Leu Ser Lys 85 90 95 acc cgt tat agt gga tta aat tta aat cag gac aag gcg acg aag ccg 336 Thr Arg Tyr Ser Gly Leu Asn Leu Asn Gin Asp Lys Ala Thr Lys Pro 100 105 110 cag aca gta caa ata gta cgg caa ggc gag gca acg ccg tac tgg ttt 384 Gin Thr Val Gin Ile Val Arg Gin Gly Glu Ala Thr Pro Tyr Trp Phe 115 120 125 aaa ttt aat cca cta tat gtg gtc gaa cag age ttc ggt acg ctg cac 432 Lys Phe Asn Pro Leu Tyr Val Val Glu Gin Ser Phe Gly Thr Leu His

297 • · σ··* ··♦» • ι»

WO 01/85772

PCT/GB01/02003

130 135 140' cgt aaa ttc cgc tat gcg cgg gca gcc tat ttc gga ctg att aaa gtg Arg Lys Phe Arg Tyr Ala Arg Ala Ala Tyr Phe Gly Leu Ile Lys Val 145 150 155 160 agt gcg caa age cat ctg aag gcg atg tgt ttg aac ctg ttg aaa gcc Ser Ala Gin Ser His Leu Lys Ala Met Cys Leu Asn Leu Leu Lys Ala 165 170 175 gcc aac aag cta agt gcg ccc get gcc gcc Ala Asn Lys Leu Ser Ala Pro Ala Ala Ala 180 185

480

528

558 <210> 150 <211> 186 <212> PRT

<213> Neisseria <400> 150 meningitidis Leu 1 Asp Leu Tyr Lys 5 Leu Gly Tyr Lys Gin 10 His Thr Arg Thr Asp 15 Ala Glu Gly Tyr Ile 20 Glu Lys Leu His Ile 25 Thr Pro Ala Asn Ala 30 His Glu Cys Lys His 35 Leu Pro Pro Leu Leu 40 Glu Gly Leu Pro Lys 45 Gly Thr Thr Val Tyr 50 Ala Asp Lys Gly Tyr 55 Asp Ser Ala Glu Asn 60 Arg Gin His Leu Glu 65 Glu His Gin Leu Leu Asp 70 Gly Ile Met Arg 75 Lys Ala Cys Arg Asn 80 Arg Pro Leu Ser Glu 85 Thr Gin Thr Lys Arg 90 Asn Arg Tyr Leu Ser 95 Lys Thr Arg Tyr Ser 100 Gly Leu -Asn Leu Asn 105 Gin Asp Lys Ala Thr 110 Lys Pro Gin Thr Val 115 Gin Ile Val Arg Gin 120 Gly Glu Ala Thr Pro 125 Tyr Trp Phe Lys Phe 130 Asn Pro Leu Tyr Val 135 Val Glu Gin Ser Phe 140 Gly Thr Leu His

298 * y '9 9

9 9 * 9

I'· 9 ’* 9

99·* 9 9 9*

PCT/GB01/02003

Val

160

Ala . 9 · ·· 9 9

9 9 9 9 9 9

WO 01/85772

Arg Lys Phe Arg Tyr Ala Arg Ala Ala Tyr Phe Gly Leu Ile Lys 145 150 155

Ser Ala Gin Ser His Leu Lys Ala Met Cys Leu Asn Leu Leu Lys 165 170 175

Ala Asn Lys Leu Ser Ala Pro Ala Ala Ala 180 185 <210> 151 <211> 1332 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> {1)..(1332) <400> 151

atg act gac ctg aac acc ctg ttt gcc aac ctc aaa caa cgc aat ccc Ala Asn Leu Lys Gin Arg Asn Pro 48 Met Thr 1 Asp Leu Asn 5 Thr Leu Phe 10 15 aat cag gag ccg ttc cat cag gcg gtt gaa gaa gtc ttc atg agt ctc 96 Asn Gin Glu Pro Phe His Gin Ala Val Glu Glu Val Phe Met Ser Leu 20 25 30 gat ccg ttt ttg gca aaa aat ccg aaa tac acc cag caa agc ctg ctg 144 Asp Pro Phe Leu Ala Lys Asn Pro Lys Tyr Thr Gin Gin Ser Leu Leu 35 40 45 gaa cgc atc gtc gaa ccc gaa cgc gtc gtg atg ttc cgc gta acc tgg 192 Glu Arg Ile Val Glu Pro Glu Arg Val Val Met Phe Arg Val Thr Trp 50 55 60 cag gac gat aaa ggg caa gtc caa gtc aac cgg ggc tac cgc gtg caa 240 Gin Asp Asp Lys Gly Gin Val Gin Val Asn Arg Gly Tyr Arg Val Gin 65 70 75 80 atg agt tcc gcc atc ggt cct tac aaa ggc ggc ctg cgc ttc cat ccg 288 Met Ser Ser Ala Ile Gly Pro Tyr Lys Gly Gly Leu Arg Phe His Pro 85 90 95 acc gtc gat ttg ggc gta ttg aaa ttc ctc get ttt gaa caa gtg ttc 336 Thr Val Asp Leu Gly Val Leu Lys Phe Leu Ala Phe Glu Gin Val Phe 100 105 110

299 • · · fc 9

9 ·'

WO 01/85772

PCT/GBO í/02003

aaa aac gcc ttg acc acc ctg cct Thr Leu Pro 120 atg ggc ggc ggc aaa ggc ggt tcc 384 Lys Asn Ala Leu Thr 115 Met Gly Gly Gly Lys 125 Gly Gly Ser gac ttc gac ccc aaa ggc aaa tcc gat gcc gaa gta atg cgc ttc tgc 432 Asp Phe Asp Pro Lys Gly Lys Ser Asp Ala Glu Val Met Arg Phe Cys 130 135 140 caa gcc ttt atg acc gaa ctc tac cgc cac atc ggc gcg gac acc gat 480 Gin Ala Phe Met Thr Glu Leu Tyr Arg His Ile Gly Ala Asp Thr Asp 145 150 155 160 gtt ccg gcc ggc gac atc ggc gta ggc ggg cgc gaa atc ggc tac ctg 528 Val Pro Ala Gly Asp Ile Gly Val Gly Gly Arg Glu Ile Gly Tyr Leu 165 170 175 ttc gga caa tac aaa aaa atc cgc aac gag ttt tet tcc gtc ctg acc 576 Phe Gly Gin Tyr Lys Lys Ile Arg Asn GlU Phe Ser Ser Val Leu Thr 180 185 190 ggc aaa ggt ttg gaa tgg ggc ggc agc ctc atc cgt ccc gaa gcg acc 624 Gly Lys Gly Leu Glu Trp Gly Gly Šer Leu Ile Arg Pro Glu Ala Thr 195 200 205 ggc tac ggc tgc gtc tat ttc gcc caa gcg atg ctg caa acc cgc aac 672 Gly Tyr Gly Cys Val Tyr Phe Ala Gin Ala Met Leu Gin Thr Arg Asn 210 215 220 gat agt ttt gaa ggc aaa cgc gtc ctg att tcc ggt tcc ggc aac gtg 720 Asp Ser Phe Glu Gly Lys Arg Val Leu Ile Ser Gly Ser Gly Asn Val 225 230 235 240 gcg caa tac gcc gcc gaa aaa gcc atc caa ctg ggc gcg aaa gtg ctg 768 Ala Gin Tyr Ala Ala Glu Lys Ala Ile Gin Leu Gly Ala Lys Val Leu 245 250 255 acc gtt tcc gac tcc aac ggc ttc gtc ctc ttc ccc gac agc ggt atg 816 Thr Val Ser Asp Ser Asn Gly Phe Val Leu Phe Pro Asp Ser Gly Met 260 - 265 270 agc gaa gcg caa ctc gcc gcc ttg atc gaa ttg aaa gaa gtc cgc cgc 864 Ser Glu Ala Gin Leu Ala Ala Leu Ile Glu Leu Lys Glu Val Arg Arg 275 280 285 gaa cgg gtt gcc acc tac gcc aaa gag caa ggt ctg caa tac ttt gaa 912 Glu Arg Val Ala Thr Tyr Ala Lys Glu Gin Gly Leu Gin Tyr Phe Glu 290 295 300

300 • · · · · · ·· · · «

9 · · · · 9 · 9 • 9 9 9 (ί 9 » 9 9 «

9 9 9 9 .· '9 9 9 »

9999 9999 · » 99'9 · fc

WO 01/85772 PCT/GB01/02003

aac Asn 305 caa aaa Gin Lys ccg tgg ggc gtc gcc gcc gaa atc gcc ctg ccc tgc gcg 960 Pro Trp Gly 310 Val Ala Ala Glu Ile 315 Ala Leu Pro Cys Ala 320 acc cag aac gaa ttg gac gaa gaa gcc gcc aaa acc ctg ttg gca aac 1008 Thr Gin Asn GlU Leu Asp Glu Glu Ala Ala Lys Thr Leu Leu Ala Asn 325 330 335 ggc tgc tac gtc gtt gcc gaa ggt gcg aat atg ccg tcg act ttg ggc 1056 Gly Cys Tyr Val Val Ala Glu Gly Ala Asn Met Pro Ser Thr Leu Gly 340 345 350 gcg gtc gag caa ttt atc aaa gcc ggc atc ctc tac gcc ccg ggc aaa 1104 Ala Val Glu Gin Phe Ile Lys Ala Gly Ile Leu Tyr Ala Pro Gly Lys 355 360 365 gcc tec aac gcc ggc ggc gta gca act tca ggt ttg gaa atg age caa 1152 Ala Ser Asn Ala Gly Gly Val Ala Thr Ser Gly Leu Glu Met Ser Gin 370 375 380 aac gcc atc cgc ctg tet tgg act cgt gaa gaa gtc gac caa cgc ctg 1200 Asn Ala Ile Arg Leu Ser Trp Thr Arg Glu Glu Val Asp Gin Arg Leu 385 390 395 400 ttc ggc atc atg caa age atc cac gaa tcc tgt ctg aaa tac ggc aaa 1248 Phe Gly Ile Met Gin Ser Ile His Glu Ser Cys Leu Lys Tyr Gly Lys 405 410 415 gtc ggc gac aca gta aac tac gtc aat ggt gcg aac att gcc ggt ttc 1296 Val Gly Asp Thr Val Asn Tyr Val Asn Gly Ala Asn Ile Ala Gly Phe 420 425 430 gtc aaa gtt gcc gat gcg atg ctg gcg caa ggc ttc 1332 val Lys Val Ala Asp Ala Met Leu Ala Gin Gly Phe 435 440

<210> 152 <211> 444 <212> PRT <213> Neisseria meningitidis <400> 152

Met Thr Asp Leu Asn Thr Leu Phe Ala Asn Leu Lys Gin Arg Asn Pro

1 5 10 15

Asn Gin Glu Pro Phe His Gin Ala Val Glu Glu Val Phe Met Ser Leu

301 • · · ♦ · * ·· · · « • · · · · « · · • .· · · '· · ·· · · ···· ···· ·.· ··· ·»

WO 01/85772 PCT/GB01/02003

20 25 30 Asp Pro Phe Leu Ala Lys Asn Pro Lys Tyr Thr Gin Gin Ser Leu Leu 35 40 45 Glu Arg Ile Val Glu Pro Glu Arg Val Val Met Phe Arg Val Thr Trp 50 55 60 Gin Asp Asp Lys Gly Gin Val Gin Val Asn Arg Gly Tyr Arg Val Gin 65 70 75 80 Met Ser Ser Ala Ile Gly Pro Tyr Lys Gly Gly Leu Arg Phe His Pro 85 90 95 Thr Val Asp Leu Gly Val Leu Lys Phe Leu Ala Phe Glu Gin Val Phe 100 105 110 Lys Asn Ala Leu Thr Thr Leu Pro Met Gly Gly Gly Lys Gly Gly Ser 115 120 125 Asp Phe Asp Pro Lys Gly Lys Ser Asp Ala Glu Val Met Arg Phe Cys 130 135 140 Gin Ala Phe Met Thr Glu Leu Tyr Arg His Ile Gly Ala Asp Thr Asp 145 150 155 160 Val Pro Ala Gly Asp Ile Gly Val Gly Gly Arg Glu Ile Gly Tyr Leu 165 170 175 Phe Gly Gin Tyr Lys Lys Ile Arg Asn Glu Phe Ser Ser Val Leu Thr 180 185 190 Gly Lys Gly Leu Glu Trp Gly Gly Ser Leu Ile Arg Pro Glu Ala Thr 195 200 205 Gly Tyr Gly Cys Val Tyr Phe Ala Gin Ala Met Leu Gin Thr Arg Asn 210 215 220 Asp Ser Phe Glu Gly Lys Arg Val Leu Ile Ser Gly Ser Gly Asn Val 225 230 - 235 240 Ala Gin Tyr Ala Ala Glu Lys Ala Ile Gin Leu Gly Ala Lys Val Leu 245 250 255 Thr Val Ser Asp Ser Asn Gly Phe Val Leu Phe Pro Asp Ser Gly Met 260 265 270 Ser Glu Ala Gin Leu Ala Ala Leu Ile Glu Leu Lys Glu Val Arg Arg

302 • 9 ·· ·φ · ·· ···· • · · · « « ·^Γ • · · 9 ** · · · · '

9 9. · 4

WOOV85772 PCT/GBO1/02003

275 280 285

Glu Arg 290 Val Ala Thr Tyr Ala 295 Lys Glu Gin Gly Leu 300 Gin Tyr Phe Glu Asn 305 Gin Lys Pro Trp Gly 310 Val Ala Ala Glu Ile 315 Ala Leu Pro Cys Ala 320 Thr Gin Asn Glu Leu 325 Asp Glu Glu Ala Ala 330 Lys Thr Leu Leu Ala 335 Asn Gly Cys Tyr Val 340 Val Ala Glu Gly Ala 345 Asn Met Pro Ser Thr 350 Leu Gly Ala Val Glu 355 Gin Phe Ile Lys Ala 360 Gly Ile Leu Tyr Ala 365 Pro Gly Lys Ala Ser 370 Asn Ala Gly Gly Val 375 Ala Thr Ser Gly Leu 380 Glu Met Ser Gin Asn 385 Ala Xle Arg Leu Ser 390 Trp Thr Arg Glu Glu 395 Val Asp Gin Arg Leu 400 Phe Gly Ile Met Gin 405 Ser Ile His Glu Ser 410 Cys Leu Lys Tyr Gly 415 Lys Val Gly Asp Thr 420 Val Asn Tyr Val Asn 425 Gly Ala Asn Ile Ala 430 Gly Phe Val Lys Val 435 Ala Asp Ala Met Leu 440 Ala Gin Gly Phe

<210> 153 <211> 867 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(867) <400> 153

atg aaa ccc ata cgg aaa gcc gtc ttc ccc gtc gca ggg atg gga aca 48 Met Lys Pro Ile Arg Lys Ala Val Phe Pro Val Ala Gly Met Gly Thr 1 5 10 15

303 ·· ·Φ Φφ 9 φΦ φφφφ

Φ Φ · · 9 9 99 φ · 9

9 9 9 '9 '9 9 · Φ ·' ΦΦΦ φ Φ * φ · φ

Φ Φ « Φ ·Φ Φ 'Φ Φ' φ

ΦΦΦΦ ΦΦΦΦ ΦΦ ΦΦΦ Φ» Φφ

WO 01/85772 PCT/GB01/02003

cgc Arg ttc ctg ccc gcc acc aag Lys gca agc ccg aaa gaa atg ctg ccc atc 96 Phe Leu Pro 20 Ala Thr Ala Ser Pro 25 Lys Glu Met Leu 30 Pro Ile gtc gac aag ccg ctg atc caa tac gcc gta gaa gaa gcc gtg gaa gcc 144 Val Asp Lys Pro Leu Ile Gin Tyr Ala Val Glu Glu Ala Val Glu Ala 35 40 45 ggc tgc acg gaa atg gtg ttt gtt acc gga cgc aac aaa cgc agc atc 192 Gly Cys Thr Glu Met Val Phe Val Thr Gly Arg Asn Lys Arg Ser Ile 50 55 60 gaa gac cat ttc gac aag gca tac gaa ctc gaa acc gag ttg gaa atg 240 Glu Asp His Phe Asp Lys Ala Tyr Glu Leu GlU Thr Glu Leu Glu Met 65 70 75 80 cgc cat aaa gac aaa ttg ttg gaa cac gtc cgc aac atc ctg ccg ccg 288 Arg His Lys Asp Lys Leu Leu Glu His Val Arg Asn Ile Leu Pro Pro 85 90 95 aac att acc tgc ctc tac atc cgt cag gcg gaa gca ctg ggc ttg gga - 336 Asn Ile Thr Cys Leu Tyr Ile Arg Gin Ala Glu Ala Leu Gly Leu Gly 100 105 110 cac gcc gtc ttg tgc gcc cgc gcc gcc atc ggc gac gaa ccc ttt gcc 384 His Ala Val Leu Cys Ala Arg Ala Ala Ile Gly Asp Glu Pro Phe Ala 115 120 125 gtg att ttg gct gac gat tta atc gat gcc caa aaa ggc gcg ctc aaa 432 Val Ile Leu Ala Asp Asp Leu Ile Asp Ala Gin Lys Gly Ala Leu Lys 130 135 140 caa atg gtc gaa gtg tac gaa cgc agc ggc aac agc att ttg ggc gta 480 Gin Met Val Glu Val Tyr Glu Arg Ser Gly Asn Ser Ile Leu Gly Val 145 150 155 160 gaa act gtc gaa ccg tcg caa acc ggc tea tac ggc atc gtc gaa acc 528 Glu Thr Val Glu Pro Ser Gin Thr Gly Ser Tyr Gly Ile Val Glu Thr

165 gaa cag ctc aaa cag ttc caa cgc 170 175 att acc ggc att gtc gaa aaa Lys ccc Pro 576 Glu Gin Leu Lys Gin Phe Gin Arg Ile 185 Thr Gly Ile Val Glu 190 180 aag ccc gaa gac gcg CCC tcc' aac ctt gcc gtc gtc gga cgc tac atc 624 Lys Pro Glu Asp Ala Pro Ser Asn Leu Ala Val Val Gly Arg Tyr Ile

195 200 205

304 ·· ’· · ·· ♦ ·φ< φφφφ φ φ φ · φ · ·· φ φ · • φφφ · · *φ φ · · · φ · '« φ • φ '· · · ^ν· '· φ φφφφ ···'< φ.φ φφφ φφ

ΦΦ

W0 01/85772 PCT/GB01/02003

ctt acc ccg cgc att ttc gac tta ctg acc gga ctg ccg cgc ggc gcg Leu Thr 210 Pro Arg Ile Phe Asp 215 Leu Leu Thr Gly Leu 220 Pro Arg Gly Ala ggc aac gaa atc cag ctt aca gac ggc atc gcc aag ctg ctc gat cac Gly 225 Asn Glu Ile Gin Leu 230 Thr Asp Gly Ile Ala 235 Lys Leu Leu Asp His 240 gaa ttt gtc cta gcg cac ccc ttt gaa ggc acg cgc tac gac tgc ggc Glu Phe Val Leu Ala 245 His Pro Phe Glu Gly 250 Thr Arg Tyr Asp Cys 255 Gly age aaa ctg ggc tat ctg gaa gcc acc gtc gcc tac ggc ctg aaa cac Ser Lys Leu Gly 260 Tyr Leu Glu Ala Thr 265 Val Ala Tyr Gly Leu 270 Lys His ccc gaa acc ggc gaa ccc ttc cgc cgc ctt ttg gaa aaa tac cgt acc Pro Glu Thr Gly Glu Pro Phe Arg Arg Leu Leu Glu Lys Tyr Arg Thr

275 280 285 gaa 867

Glu <210> 154 <211> 289 <212> PRT

<213> Neisseria meningitidis <400> 154 Met 1 Lys Pro Zle Arg 5 Lys Ala Val Phe Pro 10 Val Ala Gly Met Gly 15 Thr Arg Phe Leu Pro 20 Ala Thr Lyš Ala Ser 25 Pro Lys Glu Met Leu 30 Pro Ile Val Asp Lys 35 Pro Leu Ile Gin Tyr 40 Ala Val Glu Glu Ala 45 Val Glu Ala Gly Cys 50 Thr Glu Met val Phe ' 55 Val Thr Gly Arg Asn 60 Lys Arg Ser Ile Glu 65 Asp His Phe Asp Lys Ala 70 Tyr Glu Leu Glu 75 Thr Glu Leu Glu Met 80 Arg His Lys Asp Lys 85 Leu Leu Glu His Val 90 Arg Asn Ile Leu Pro 95 Pro

305

r·* ·» 9» .9 *· · 9 9 • · 9 9 9 9 ·« 'fc 9 • 9 9 9 9 • 9 -9 · · '· • · 99 9 9 9 9 99 • · 9 9 9 9 9 •

WO «1/85772 PCT/GB01/02003

Asn Ile Thr Cys 100 Leu Tyr Ile Arg Gin 105 Ala Glu Ala Leu Gly 110 Leu Gly His Ala Val 115 Leu Cys Ala Arg Ala 120 Ala Ile Gly Asp Glu 125 Pro Phe Ala Val Ile 130 Leu Ala Asp Asp Leu 135 Ile Asp Ala Gin Lys 140 Gly Ala Leu Lys Gin 145 Met Val Glu Val Tyr 150 Glu Arg Ser Gly Asn 155 Ser Ile Leu Gly Val 160 Glu Thr Val Glu Pro Ser 165 Gin Thr Gly Ser 170 Tyr Gly Ile Val Glu 175 Thr Glu Gin Leu Lys 180 Gin Phe Gin Arg Ile 185 Thr Gly Ile Val Glu 190 Lys Pro Lys Pro Glu 195 Asp Ala Pro Ser Asn 200 Leu Ala Val Val Gly 205 Arg Tyr Ile Leu Thr 210 Pro Arg Ile Phe Asp 215 Leu Leu Thr Gly Leu 220 Pro Arg Gly Ala Gly 225 Asn Glu Ile Gin Leu 230 Thr Asp Gly Ile Ala 235 Lys Leu Leu Asp His 240 Glu Phe Val Leu Ala His 245 Pro Phe Glu Gly 250 Thr Arg Tyr Asp Cys 255 Gly Ser Lys Leu Gly 260 Tyr Leu Glu Ala Thr 265 Val Ala Tyr Gly Leu 270 Lys His Pro Glu Thr Gly Glu Pro Phe Arg Arg Leu Leu Glu Lys Tyr Arg Thr

275 280 285

Glu <210> 155 <211> 876 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS

306

WO 01/85772 PCT/GB01/02003 <222> (1)..(876) <400> 155

ttg acc gtc ega Arg acg Thr 5 aaa aag acg gcg cat tat acc cta ttc cat tcc 48 Leu 1 Thr Val Lys Lys Thr Ala His 10 Tyr Thr Leu Phe His 15 Ser gac ega aaa ccg aac atg act act ctc aaa ccc gcc ctg ccc get tat 96 Asp Arg Lys Pro Asn Met Thr Thr Leu Lys Pro Ala Leu Pro Ala Tyr 20 25 30 ctg gac aac atc cgc atc atc ctc acg cgc acc age cat ccc gcc aac 144 Leu Asp Asn Ile Arg Ile Ile Leu Thr Arg Thr Ser His Pro Ala Asn 35 40 ⁴⁵ atc ggc tet gcc gcg cgc gcg atg aaa aca atg ggt ctg cac aaa ctg 192 Ile Gly Ser Ala Ala Arg Ala Met Lys Thr Met Gly Leu His Lys Leu 50 55 60 acc atc gtc gcc cca aat ctg atg gca acg ccg atg acg gaa aac ccg 240 Thr Ile Val Ala Pro Asn Leu Met Ala Thr Pro Met Thr Glu Asn Pro 65 70 75 80 ccc gtg ttt gac ccg gag cat cct caa tcg ttt aaa tta ccg gaa gaa 288 Pro Val Phe Asp Pro Glu His Pro Gin Ser Phe Lys Leu Pro Glu Glu 85 90 95 age ttc atc ctc get tcc ggc gcg gca gac gtt ttg gaa aat gcc acc 336 Ser Phe Ile Leu Ala Ser Gly Ala Ala Asp Val Leu Glu Asn Ala Thr 100 105 110 att gcc get tet ttg gac gaa gcc ctt gcc gac acc acc atc gcc tgc 384 Ile Ala Ala Ser Leu Asp Glu Ala Leu Ala Asp Thr Thr Ile Ala Cys 115 120 125 gcc ctg acc age cgc cgc cgc gaa att act gcg ccg ctg caa acc ccg 432 Ala Leu Thr Ser Arg Arg Arg Glu Ile Thr Ala Pro Leu Gin Thr Pro 130 135 140 cgc gat ttg gta tcc gaa tta ctg cag acc gca aac ega ggc gag aaa 480 Arg Asp Leu Val Ser Glu -Leu Leu Gin Thr Ala Asn Arg Gly Glu Lys 145 150 155 160 gtg gca ctg gtt ttc ggc aac gag act ttc ggc ttg age atc gaa gaa 528 Val Ala Leu Val Phe Gly Asn Glu Thr Phe Gly Leu Ser Ile Glu Glu 165 170 175 gtc caa gcc tgc aac ega ctg atg acc atc aac ggc aat ccc gac tat 576

307 ·· ·· ·* • · ·, · » « • « · · • « ♦ · ·

WO 01/85772 Val Gin Ala Cys 180 Asn Arg Leu Met Thr 185 Ile ttc tcg ctc aac ctc gcc caa gcc gtg cag Phe Ser Leu 195 Asn Leu Ala Gin Ala 200 Val Gin ttc agc caa acc ggt tcg ccc atg acc cat Phe Ser 210 Gin Thr Gly Ser Pro 215 Met Thr His gct gcg acc cac gag caa atc aaa ggc atg Ala 225 Ala Thr His Glu Gin 230 Ile Lys Gly Met gtg atg aac gac atc ggc ttt ttc aac cgc Val Met Asn Asp Ile 245 Gly Phe Phe Asn Arg 250 atg cgc cgt atg cag agc Ctg ttc gga cgc Met Arg Arg Met 260 Gin Ser Leu Phe Gly 265 Arg gac atc gat atc ctg cgc ggt ttt ttc aat Asp Xle Asp 275 Ile Leu Arg Gly Phe 280 Phe Asn

cat aaa aaa gac His Lys Lys Asp

290

PCT/GB01/02003 Asn Gly Asn Pro 190 Asp Tyr gtc gtg tgc tac gaa atc 624 Val Val Cys 205 Tyr Glu Ile ctt caa caa gaa gac cac 672 Leu Gin 220 Gin Glu Asp His gtc gcc cac atg gaa agc 720 Val 235 Ala His Met Glu Ser 240 cgc aac ggc gag cgt ctg 768 Arg Asn Gly Glu Arg 255 Leu gcc aac acg caa acc gaa 816 Ala Asn Thr Gin 270 Thr Glu acc gtc agc cat cgt atc 864 Thr Val Ser 285 His Arg Zle 876.

<210> 156 <211> 292 <212> PRT <213> Neisseria meningitidis <400> 156

Leu 1 Thr Val Arg Thr 5 Lys Lys Thr Ala His 10 Tyr Thr Leu Phe His 15 Ser Asp Arg Lys Pro 20 Asn Met Thr Thr Leu 25 Lys Pro Ala Leu Pro 30 Ala Tyr Leu Asp Asn 35 Ile Arg Ile Ile Leu 40 Thr Arg Thr Ser His 45 Pro Ala Asn Ile Gly Ser Ala Ala Arg Ala Met Lys Thr Met Gly Leu His Lys Leu

308

• · • • ··· • ·. • • • • · · · • 0 • • • 0 0 0 • · • · 9 9 ·« • 99 9 9 9 99 9 0· ·· • « • · • · · • · · • · · WO 01/85772 PCT/GB01/02003 50 55 60 Thr Ile Val Ala Pro Asn Leu Met Ala Thr Pro Met Thr Glu Asn Pro 65 70 75 80 Pro Val Phe Asp Pro Glu His Pro Gin Ser Phe Lys Leu Pro Glu Glu 85 90 95 Ser Phe Ile Leu Ala Ser Gly Ala Ala Asp Val Leu Glu Asn Ala Thr 100 105 110 Ile Ala Ala Ser Leu Asp Glu Ala Leu Ala Asp Thr Thr Ile Ala Cys 115 120 125 Ala Leu Thr Ser Arg Arg Arg Glu Ile Thr Ala Pro Leu Gin Thr Pro 130 135 140 Arg Asp Leu Val Ser Glu Leu Leu Gin Thr Ala Asn Arg Gly Glu Lys 145 150 155 160 Val Ala Leu Val Phe Gly Asn Glu Thr Phe Gly Leu Ser Ile Glu Glu 165 170 175 Val Gin Ala Cys Asn Arg Leu Met Thr Ile Asn Gly Asn Pro Asp Tyr 180 185 190 Phe Ser Leu Asn Leu Ala Gin Ala Val Gin Val Val Cys Tyr Glu Ile 195 200 205 Phe Ser Gin Thr Gly Ser Pro Met Thr His Leu Gin Gin Glu Asp His 210 ' 215 220 Ala Ala Thr His Glu Gin Ile Lys Gly Met Val Ala His Met Glu Ser 225 230 235 240 Val Met Asn Asp Ile Gly Phe Phe Asn Arg Arg Asn Gly Glu Arg Leu 245 250 255 Met Arg Arg Met Gin Ser Leu Phe Gly Arg Ala Asn Thr Gin Thr Glu 260 265 270 Asp Ile Asp Ile Leu Arg Gly Phe Phe Asn Thr Val Ser His Arg Ile 275 280 285 His Lys Lys Asp

290

309 ···· • ·

WO 01/85772

PCT/GB01/02003 <210> 157 <211> 1008 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1008) <400> 157

atg tcc atc aaa atc ctg att Leu Ile att tcc ccc agt tgg ata ggc gac tgc Cys 48 Met 1 Ser Ile Lys Ile 5 Ile Ser Pro 10 Ser Trp Ile Gly Asp 15 gtg atg acc cag ccc ttg ttc cgc cgt ttg aag gaa ctt cac ccc ggt 96 Val Met Thr Gin Pro Leu Phe Arg Arg Leu Lys Glu Leu His Pro Gly 20 25 30 tgc acg att gat gtg ttc gca ccg aag tgg tcg atg gcg gtg ttť gag 144 Cys Thr Ile Asp Val Phe Ala Pro Lys Trp Ser Met Ala Val Phe Glu 35 40 45 cgt atg ccg gaa gtg aat gag att ctt gaa aat ccg ttc gga cac ggt 192 Arg Met Pro Glu Val Asn Glu Ile Leu Glu Asn Pro Phe Gly His Gly 50 55 60 gcg ttg gag ttg aaa cgc cgt tgg cgg gtc ggt agg gat ttg ggg cgg 240 Ala Leu Glu Leu Lys Arg Arg Trp Arg val Gly Arg Asp Leu Gly Arg 65 70 75 80 cgc gga tac gat cag gtt atc gtg ttg ccc ggt tet ttg aaa tcg gca 288 Arg Gly Tyr Asp Gin Val Ile Val Leu Pro Gly ser Leu Lys Ser Ala 85 90 95 atc atc gcg ctg gcg aca ggt atc ggt aaa agg acg ggt tat gtc ggt 336 Ile Ile Ala Leu Ala Thr Gly Ile Gly Lys Arg Thr Gly Tyr Val Gly 100 105 110 gaa agc cgt tat ttt ctg -ttg aac gat ata cgc agg ctg gat aag gaa 384 Glu Ser Arg Tyr Phe Leu Leu Asn Asp Ile Arg Arg Leu Asp Lys Glu 115 120 125 cgt ctg cct ttg atg gtg gac aga tat acg gct ctc gcg cat ccg agc 432 Arg Leu Pro Leu Met Val Asp Arg Tyr Thr Ala Leu Ala His Pro Ser 130 135 140 cag gag gat ttt gac ggg cat tcg gga ttc ccc gag ttt tcc att gat 480

310 *· • · · · φ · * · ·.» · • · ···· · · • · · ·. '·· · ···· · · « · φ

WO 01/85772 PCT/GB01/02003

Gin 145 Glu Asp Phe Asp Gly 150 His Ser Gly Phe Pro 155 Glu Phe Ser Ile Asp 160 gaa cgg cgg cgg gaa att tet gtc gaa acc ttt ggc ttg gat att gga 528 Glu Arg Arg Arg Glu 165 Ile Ser Val Glu Thr 170 Phe Gly Leu Asp Ile 175 Gly aag cct gtt ctg get ttt tgt ccg ggt gcg gaa ttc ggg ccg gca aag 576 Lys Pro Val Leu 180 Ala Phe Cys Pro Gly 185 Ala Glu Phe Gly Pro 190 Ala Lys cgt tgg ccg aca agg tat ttt gcc gag ttg ggc aaa cat tat tcg gcg 624 Arg Trp Pro 195 Thr Arg Tyr Phe Ala 200 Glu Leu Gly Lys His 205 Tyr Ser Ala gcg ggt tgg cag gtt tgg ctg ttc ggt tcg caa aaa gat gat gaa att 672 Ala Gly 210 Trp Gin Val Trp Leu 215 Phe Gly Ser Gin Lys 220 Asp Asp Glu Ile gcc gag gaa atc aac tgc ctt tea gac ggt atg tgt gtc aat ttg tgc 720 Ala 225 Glu Glu Ile Asn Cys 230 Leu Ser Asp Gly Met 235 Cys Val Asn Leu Cys 240 ggc aaa acc gat ttg tcg cag gca atg gat ttg ctg tcg ttg gcg gac 768 Gly Lys Thr Asp Leu 245 Ser Gin Ala Met Asp 250 Leu Leu Ser Leu Ala 255 Asp acg gtc gtg tgc aac gac age ggg ctg atg cac ttg gcg gcg gca ttg 816 Thr Val Val Cys 260 Asn Asp Ser Gly Leu 265 Met His Leu Ala Ala 270 Ala Leu ggc agg aag gtg gtg gcg gtt tac ggt tet tcc age ccg acg cat acg 864 Gly Arg Lys 275 Val Val Ala Val Tyr 280 Gly Ser Ser Ser Pro 285 Thr His Thr ccg cct ttg age gac agg gcg aaa atc gtc age ctg cac ttg gaa tgt 912 Pro Pro 290 Leu Ser Asp Arg Ala 295 Lys Ile Val Ser Leu 300 His Leu Glu Cys tcg ccc tgt ttt aag cgg, gaa tgt ccg ctg ggg cat acc gac tgc ctc 960 Ser 305 Pro Cys Phe Lys Arg' 310 Glu Cys Pro Leu Gly 315 His Thr Asp Cys Leu 320 aac agg ctg tat ccc gag aag att gtg cag gcg gtt gaa gag gcg gta 1008 Asn Arg Leu Tyr Pro 325 Glu Lys Ile Val Gin 330 Ala Val Glu Glu Ala 335 Val

311 ·· ···· ·· ··»« ·*·«

WO 01/85772 PCT/GBO1/02003 <210> 158 <211> 336 <212> PRT <213> Neisseria meningitidis <400> 158

Met 1 Ser Xle Lys Xle Leu Xle Xle Ser Pro Ser Trp Xle Gly Asp Cys 5 10 15 Val Met Thr Gin 20 Pro Leu Phe Arg Arg Leu 25 Lys Glu Leu His Pro 30 Gly Cys Thr Xle 35 Asp Val Phe Ala Pro Lys Trp 40 Ser Met Ala Val Phe 45 Glu Arg Met 50 Pro Glu Val Asn Glu Xle Leu Glu 55 Asn Pro Phe Gly His 60 Gly Ala 65 Leu Glu Leu Lys Arg Arg Trp Arg Val 70 Gly Arg Asp Leu Gly 75 Arg 80 Arg Gly Tyr Asp Gin 85 Val Xle Val Leu Pro 90 Gly Ser Leu Lys Ser 95 Ala Xle Xle Ala Leu 100 Ala Thr Gly Xle Gly Lys 105 Arg Thr Gly Tyr Val 110 Gly Glu Ser Arg 115 Tyr Phe Leu Leu Asn Asp Ile 120 Arg Arg Leu Asp Lys 125 Glu Arg Leu 130 Pro Leu Met Val Asp Arg Tyr Thr 135 Ala Leu Ala His Pro 140 Ser Gin 145 Glu Asp Phe Asp Gly His Ser Gly Phe 150 Pro Glu Phe Ser Ile 155 Asp 160 Glu Arg Arg Arg Glu 165 Ile Ser Val Glu Thr 170 Phe Gly Leu Asp Ile 175 Gly Lys Pro Val Leu 180 Ala Phe,'Cys Pro Gly Ala 185 Glu Phe Gly Pro Ala 190 Lys Arg Trp Pro 195 Thr Arg Tyr Phe Ala Glu Leu 200 Gly Lys His Tyr Ser 205 Ala Ala Gly Trp Gin Val Trp Leu Phe Gly Ser Gin Lys Asp Asp Glu Ile

210 215 220

312 ·· ·· ·· * ·» ···· • · · · · · ·· ·· · • · · · · ··· • · · · · ···» · • · · · · · 0 e _e ···· ··*· ·· «·· ·· ··

PCT/GBO1/02003

WO 01/85772

Ala Glu Glu Xle Asn Cys Leu Ser Asp Gly Met Cys Val Asn Leu Cys 225 230 235 240 Gly Lys Thr Asp Leu Ser Gin Ala Met Asp Leu Leu Ser Leu Ala Asp 245 250 255 Thr Val Val Cys Asn Asp Ser Gly Leu Met His Leu Ala Ala Ala Leu 260 265 270 Gly Arg Lys Val Val Ala Val Tyr Gly Ser Ser Ser Pro Thr His Thr 275 280 285 Pro Pro Leu Ser Asp Arg Ala Lyš Ile Val Ser Leu His Leu Glu Cys 290 295 300 Ser Pro Cys Phe Lys Arg Glu cys Pro Leu Gly His Thr Asp Cys Leu 305 310 315 320 Asn Arg Leu Tyr Pro Glu Lys Ile Val Gin Ala Val Glu Glu Ala Val

325

330

335 <210> 159 <211> 381 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(381, <400> 159

atg gaa cct tcc tcc tac gcg gca gaa aaa aaa gga aaa ggc ggc atc 48 Met Glu Pro Ser Ser Tyr Ala Ala Glu Lys Lys Gly Lys Gly Gly Ile 1 5 10 15 agg cgc gtc atc aac gca ttc ggc tat tcg ata gac ggc atc gcc gcc 96 Arg Arg Val Ile Asn Ala Phe Gly Tyr Ser Ile Asp Gly Ile Ala Ala 20 25 30 gcc tac cgt tac gaa gcg gca ttc cgt cag gtt ttg tgg ctg aac gcg 144 Ala Tyr Arg Tyr Glu Ala Ala Phe Arg Gin Val Leu Trp Leu Asn Ala 35 40 45 ctg ctg gtg tgc gcg gca ttt ttt tgg gtt tcc gaa aag tcc ctc cgc 192 Leu Leu Val Cys Ala Ala Phe Phe Trp Val Ser Glu Lys Ser Leu Arg

313

WO 01/85772

PCT/GB01/02003

ctg ccg ttg att atc gcg tet ttt gtg tcg gtc att gtc gaa ctg ttc 240 Leu 65 Pro Leu Ile Ile Ala 70 Ser Phe Val Ser Val 75 Ile Val Glu Leu Phe 80 aac act gcc gtc gaa gcc gcc gtc gat cat act tcg act gaa aaa cac 288 Asn Thr Ala Val Glu Ala Ala Val Asp His Thr Ser Thr Glu Lys His 85 90 95 gag ctg gct aaa cgc gcc aaa gac gca ggt tet gct gca caa ttg ttc 336 Glu Leu Ala Lys Arg Ala Lys Asp Ala Gly Ser Ala Ala Gin Leu Phe 100 105 110 gcg atg ctg atg tta gcg gcg gtt tgg ctg tcc gcc ctg ttc ggg 381 Ala Met Leu Met Leu Ala Ala Val Trp Leu Ser Ala Leu Phe Gly

115 120 125 <210> 160 <211> 127 <212> PRT

<213> Neisseria meningitidis <400> 160 Met Glu Pro Ser Ser Tyr Ala Ala Glu Lys Lys Gly Lys Gly Gly Ile 1 5 10 15 Arg Arg Val Ile Asn Ala Phe Gly Tyr Ser Ile Asp Gly Ile Ala Ala 20 25 30 Ala Tyr Arg Tyr Glu Ala Ala Phe Arg Gin Val Leu Trp Leu Asn Ala 35 40 45 Leu Leu Val Cys Ala Ala Phe Phe Trp Val Ser Glu Lys Ser Leu Arg 50 55 60 Leu Pro Leu Ile Ile Ala Ser Phe Val Ser Val Ile Val Glu Leu Phe 65 70 75 80 Asn Thr Ala Val Glu Ala .Ala Val Asp His Thr Ser Thr Glu Lys His 85 90 95 Glu Leu Ala Lys Arg Ala Lys Asp Ala Gly Ser Ala Ala Gin Leu Phe 100 105 110 Ala Met Leu Met Leu Ala Ala Val Trp Leu Ser Ala Leu Phe Gly

115 120 125

314

99 9999

9 9

9 · · 9 · • · · ·

WO 01/85772 PCT/GB01/02003 <210> 161 <211> 990 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(990) <400> 161

atg Met 1 aaa aaa gaa Glu age cgc ccc gcg ttt gac gcg gca gcc gta ttt gac 48 Lys Lys Ser 5 Arg Pro Ala Phe Asp 10 Ala Ala Ala Val Phe 15 Asp gcg gca gcc gta ccg gta tcc gac age ggg ttt gcc gcc aat gca aat 96 Ala Ala Ala Val Pro Val Ser Asp Ser Gly Phe Ala Ala Asn Ala Asn 20 25 30 gtc cgc cgt ttt gtg gac gat gaa gtc ggg aaa ggg gat ttt tcc cgg 144 Val Arg Arg Phe val Asp Asp Glu Val Gly Lys Gly Asp Phe Ser Arg 35 40 45 gcg gaa tgg cag gat ttt ttt gac aaa gcg gct tac aag gcg gac atc 192 Ala Glu Trp Gin Asp Phe Phe Asp Lys Ala Ala Tyr Lys Ala Asp Ile 50 55 60 gtc aag att atg cac cgc ccc tcc aca tcg cgt ccg tgg tat gtg ttc 240 Val Lys Ile Met His Arg Pro Ser Thr Ser Arg Pro Trp Tyr Val Phe 65 70 75 80 cgc acg gga aat tcg ggc aag gcg aaa ttt cgc ggc gcg cgc cgg ttt 288 Arg Thr Gly Asn Ser Gly Lys Ala Lys Phe Arg Gly Ala Arg Arg Phe 85 90 95 tat gcg gaa aac cgc gcg ctt atc gat gat gtg gcg caa aaa tac ggc 336 Tyr Ala Glu Asn Arg Ala Leu Ile Asp Asp Val Ala Gin Lys Tyr Gly 100 105 110 gtg cct gcc gaa ctt atc -gtg gcg gtt atc ggg att gaa acg aat tac 384 Val Pro Ala Glu Leu Ile Val Ala Val Ile Gly Ile Glu Thr Asn Tyr 115 120 125 ggc aaa aat acg ggc agt ttc cgt gtg gcg gac gca ttg gcg acc tta 432 Gly Lys Asn Thr Gly Ser Phe Arg Val Ala Asp Ala Leu Ala Thr Leu 130 135 140

315

WO 01/85772

PCT/GBO1/02003

ggc ttt gat tac ccc cgc cgc gcc ggg ttt Phe ttc caa aaa gaa ttg gtc Val 160 480 Gly 145 Phe Asp Tyr Pro Arg Arg Ala 150 Gly Phe 155 Gin Lys Glu Leu gag ctt tta aag ctg gca aaa gaa gaa ggc ggc gat gtt ttc gcc ttt 528 Glu Leu Leu Lys Leu Ala Lys Glu Glu Gly Gly Asp Val Phe Ala Phe 165 170 175 aaa ggc age tat gcg ggc gca atg ggg atg ccg caa ttt atg cct tcg 576 Lys Gly Ser Tyr Ala Gly Ala Met Gly Met Pro Gin Phe Met Pro Ser 180 185 190 age tac cgg aaa tgg gcg gtg gat tat gac ggg gac gga cat cgg gac 624 Ser Tyr Arg Lys Trp Ala Val Asp Tyr Asp Gly Asp Gly His Arg Asp 195 200 205 ata tgg ggc aat gtt ggc gat gtc gcg gca tcg att gcc aat tat atg 672 Ile Trp Gly Asn Val Gly Asp Val Ala Ala Ser Ile Ala Asn Tyr Met 210 215 220 aag cag cac ggt tgg cgc acg ggc ggg aaa ata ctg gtg tet gca aca 720 Lys Gin His Gly Trp Arg Thr Gly Gly Lys Ile Leu Val Ser Ala Thr 225 230 235 240 ttg gcg ccg ggt gcg gat gtt cag gca atc att ggc gaa aaa acc gcc 768 Leu Ala Pro Gly Ala Asp Val Gin Ala Ile Ile Gly Glu Lys Thr Ala 245 250 255 ctg acg cgg acg gtg gcg gat ttg aag gcg tac ggc atc atc ccc ggc 816 Leu Thr Arg Thr Val Ala Asp Leu Lys Ala Tyr Gly Ile Ile Pro Gly 260 265 270 gaa gag ctt gcc gat gat gaa aag gcg gtt ttg ttc aaa ctg gaa acc 864 Glu Glu Leu Ala Asp Asp Glu Lys Ala Val Leu Phe Lys Leu Glu Thr 275 280 285 gca ccc ggc gtg ttt gaa tat tat ttg ggc ttg aac aat ttt tat acg 912 Ala Pro Gly Val Phe Glu Tyr Tyr Leu Gly Leu Asn Asn Phe Tyr Thr 290 295 300 gta tgg cag tac aat cac agt cgg atg tat gta acg gcg gtc agg gac 960 Val Trp Gin Tyr Asn His Ser Arg Met Tyr Val Thr Ala Val Arg Asp 305 310 315 320 att gcc aat tcg ctt ggc ggc ccg gga ttg 990 Ile Ala Asn Ser Leu Gly Gly Pro Gly Leu 325 330

316 tta s · * «

WO 01/85772 PCT/GB01/02003 <210> 162 <211> 330 <212> PRT <213> Neisseria meningitidis <400> 162

Met 1 Lys Lys Glu Ser 5 Arg Pro Ala Phe Asp Ala Ala Ala Val Phe Asp 10 15 Ala Ala Ala Val Pro Val Ser Asp Ser Gly Phe Ala Ala Asn Ala Asn 20 25 30 Val Arg Arg Phe Val Asp Asp Glu Val Gly Lys Gly Asp Phe Ser Arg 35 40 45 Ala Glu Trp Gin Asp Phe Phe Asp Lys Ala Ala Tyr Lys Ala Asp Ile 50 55 60 Val Lys Ile Met His Arg Pro Ser Thr Ser Arg Pro Trp Tyr Val Phe 65 70 75 80 Arg Thr Gly Asn Ser Gly Lys Ala Lys Phe Arg Gly Ala Arg Arg Phe 85 90 95 Tyr Ala Glu Asn Arg Ala Leu Ile Asp Asp Val Ala Gin Lys Tyr Gly 100 105 110 Val Pro Ala Glu Leu Ile Val Ala Val Ile Gly Ile Glu Thr Asn Tyr 115 120 125 Gly Lys Asn Thr Gly Ser Phe Arg Val Ala Asp Ala Leu Ala Thr Leu 130 135 140 Gly Phe Asp Tyr Pro Arg Arg Ala Gly Phe Phe Gin Lys Glu Leu Val 145 150 155 160 Glu Leu Leu Lys Leu Ala Lys Glu Glu Gly Gly Asp Val Phe Ala Phe 165 170 ¹⁷⁵ Lys Gly Ser Tyr Ala Gly-Ala Met Gly Met Pro Gin Phe Met Pro Ser 180 185 190 Ser Tyr Arg Lys Trp Ala Val Asp Tyr Asp Gly Asp Gly His Arg Asp 195 200 205 Ile Trp Gly Asn Val Gly Asp Val Ala Ala Ser Ile Ala Asn Tyr Met

210 215 220

317

WO 01/85772

PCT/GBO1/O2OO3

Lys 225 Gin His Gly Trp Arg Thr 230 Gly Gly Lys Ile Leu Val Ser Ala Thr 235 240 Leu Ala Pro Gly Ala Asp Val Gin Ala Ile Ile Gly Glu Lys Thr Ala 245 250 255 Leu Thr Arg Thr Val Ala Asp Leu Lys Ala Tyr Gly Ile Ile Pro Gly 260 265 270 Glu Glu Leu Ala Asp Asp Glu Lys Ala Val Leu Phe Lys Leu Glu Thr 275 280 285 Ala Pro Gly Val Phe Glu Tyr Tyr Leu Gly Leu Asn Asn Phe Tyr Thr 290 295 300 Val Trp Gin Tyr Asn His Ser Arg Met Tyr Val Thr Ala Val Arg Asp 305 310 315 320 Ile Ala Asn Ser Leu Gly Gly Pro Gly Leu 325 330

<210> 163 <211> 1773 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1773) <400> 163 atg agt atc gtg ctg cac ggc gtg gcg gcg ggc aaa ggc att gcc gtc

Met Ser Xle Val Leu His Gly Val Ala Ala Gly Lys Gly Xle Ala Val

15 10 15 ggt tgc gcc cac ctg att gcg cgc ggt acg gag gaa gtg ccg cag tat

Gly Cys Ala His Leu Ile .Ala Arg Gly Thr Glu Glu Val Pro Gin Tyr

20 ' 25 30 gat gtt gcg gag gcg gac acc gat gcc gaa gcc gaa cgt ttc gat gcc

Asp Val Ala Glu Ala Asp Thr Asp Ala Glu Ala Glu Arg Phe Asp Ala

35 40 45 gcc gtc aaa gcc acg cgc aaa gag ttg gaa cag ctc cgc agc gcg att

Ala Val Lys Ala Thr Arg Lys Glu Leu Glu Gin Leu Arg Ser Ala Ile

144

192

318 • · · · · ··· · · · • ···· ··· • · · · · · · · · · • · ··· ···· ··«· *··· ·· ··· ·· ··

WO 01/85772 50 55 60 PCT/GB01/02003 ccc gaa aac gcc ccg acc gag ttg ggc gcg ttc atc tcg ctg cac ctg 240 Pro Glu Asn Ala Pro Thr Glu Leu Gly Ala Phe Ile Ser Leu His Leu 65 70 75 80 atg ctc ttg acc gat gtt acc ttg tcg cgc gaa ccc gtc gat att tta 288 Met Leu Leu Thr Asp Val Thr Leu Ser Arg Glu Pro Val Asp Ile Leu 85 90 95 agg gaa caa aaa atc aac gcc gag tgg gca ttg aag cag cag age gac 336 Arg Glu Gin Lys Ile Asn Ala Glu Trp Ala Leu Lys Gin Gin Ser Asp 100 105 110 aaa ctc gcc gcc caa ttc gac aat atg gac gat gcc tat ttg cgc gaa 384 Lys Leu Ala Ala Gin Phe Asp Asn Met Asp Asp Ala Tyr Leu Arg Glu 115 120 125 cgc aag cag gat atg ctg caa gtc gtc cgc cgc atc cac aac aac ctg 432 Arg Lys Gin Asp Met Leu Gin Val Val Arg Arg Ile His Asn Asn Leu 130 135 140 atc ggg cag ggc aac gag ttg gaa gtt gcc gac aac ctg ttt gac gaa 480 Ile Gly Gin Gly Asn Glu Leu Glu Val Ala Asp Asn Leu Phe Asp Glu 145 150 155 160 acc gtt ctg att gca aac gac ctt tcg ccc gcc gac acg gtt ttg ttt 528 Thr Val Leu Ile Ala Asn Asp Leu Ser Pro Ala Asp Thr Val Leu Phe 165 170 175 aaa gag cag cgc att gcc gcc ttc gtt acc gat gcc ggc ggc ccc acc 576 Lys Glu Gin Arg Ile Ala Ala Phe Val Thr Asp Ala Gly Gly Pro Thr 180 185 190 ggg cat acg gcg att ttg ggc agg age ttg gac atc ccg tcc gtc gtc 624 Gly His Thr Ala Ile Leu Gly Arg Ser Leu Asp Ile Pro Ser Val Val 195 200 205 ggg ctg cac aac gcg cgc aaa ctg att acc gag ggc gaa acg gtc att 672 Gly Leu His Asn Ala Arg Lys Leu Ile Thr Glu Gly Glu Thr Val Ile 210 '215 220 gtg gac ggt atc aac ggc gtg ttg att atc gcg ccg gat gag tcg gtg 720 Val Asp Gly Ile Asn Gly Val Leu Ile Ile Ala Pro Asp Glu Ser Val 225 230 235 240 ttg aac gaa tac cgc cgc cgt gcc cgc gaa tac cgc age cac aaa cgc 768 Leu Asn Glu Tyr Arg Arg Arg Ala Arg Glu Tyr Arg Ser His Lys Arg

319 β · e · · 9 φ e

WO 01/85772 PCT/GB01/02003 245 .250 255 gat ttg aac aag ctc aaa aaa acc gcc gcc gcc acc get gac ggg gtc 816 Asp Leu Asn Lys Leu Lys Lys Thr Ala Ala Ala Thr Ala Asp Gly Val 260 265 270 tgc atc gag ctt gtg ggc aat ata gaa tcc gcc gaa gac gtg aaa ccg 864 Cys Ile Glu Leu Val Gly Asn Ile Glu Ser Ala Glu Asp Val Lys Pro 275 280 285 ctg cac aac ctc ggc gca gac ggc atc ggg ctg ttc cgc age gag ttt 912 Leu His Asn Leu Gly Ala Asp Gly Ile Gly Leu Phe Arg Ser Glu· Phe 290 295 300 ctt tac ctg aac cgc gat acg atg ccg tet gaa gac gag cag tac gaa 960 Leu Tyr Leu Asn Arg Asp Thr Met Pro Ser Glu Asp Glu Gin Tyr Glu 305 310 315 320 gtg tac age gcg att gtc aaa aaa atg aaa ggc aaa age gta acg ata 1008 Val Tyr Ser Ala Ile Val Lys Lys Met Lys Gly Lys Ser Val Thr Ile 325 330 335 cgg aca gtc gat ttg ggt gtg gac aaa aac ccg cgc tgg ttc ggg aaa 1056 Arg Thr Val Asp Leu Gly Val Asp Lys Asn Pro Arg Trp Phe Gly Lys 340 345 350 aac age acg ccc aac ggc age ctc aac ccc gcg ctg ggc atg acc ggc 1104 Asn Ser Thr Pro Asn Gly Ser Leu Asn Pro Ala Leu Gly Met Thr Gly 355 360 365 atc cgc ctg tgc ctt gcc gaa ccg gtc atg ttc cgc acc cag atg cgc 1152 Ile Arg Leu Cys Leu Ala Glu Pro Val Met Phe Arg Thr Gin Met Arg 370 375 380 gcc atc ctc cgc gcc gcc gcc cac ggc ccc gtg cgg atg atg tgg ccg 1200 Ala Ile Leu Arg Ala Ala Ala His Gly Pro Val Arg Met Met Trp Pro 385 390 395 400 atg att acc tcc gta tcc gaa gtg cgc cag tgc ctc atc cac ctc gac 1248 Met Ile Thr Ser Val Ser ,Glu Val Arg Gin Cys Leu Ile His Leu Asp 405 410 415 acc gcg caa cgc cag ctt gcc gaa cgc ggc gat gcc ttc ggt aaa gtc 1296 Thr Ala Gin Arg Gin Leu Ala Glu Arg Gly Asp Ala Phe Gly Lys Val 420 425 430 ggc atc ggc tgt atg att gaá att ccg tet gcc gcg ctg acc gtc ggc 1344 Gly Ile Gly Cys Met Ile Glu Ile Pro Ser Ala Ala Leu Thr Val Gly

320 ·· «« • · · · ·

WO 01/85772 PCT/GB01/02003

435 440 445 agt att ttg aaa ctg gtc gat ttc atc tcc gtc ggt acc aac gac ctg 1392 Ser Ile Leu Lys Leu Val Asp Phe Ile Ser Val Gly Thr Asn Asp Leu 450 455 460 att caa tac atc ttg tcc gtc gat cgc ggc gac gac agc gtc agc cac 1440 Ile Gin Tyr Ile Leu Ser Val Asp Arg Gly Asp Asp Ser Val Ser His 465 470 475 480 ctc tac cag ccc ggc cat ccc gcc gtg ctg aaa atg ctg caa cac gtc 1488 Leu Tyr Gin Pro Gly His Pro Ala Val Leu Lys Met Leu Gin His Val 485 490 495 atc cgt acc gcc aac cgc atg gac aaa gac gta tcc gta tgc ggc gag 1536 Ile Arg Thr Ala Asn Arg Met Asp Lys Asp Val Ser Val Cys Gly Glu 500 505 510 atg gcg ggc gat acc gcg ttt acc cgc gtt tta ttg ggt atg ggg ctg 1584 Met Ala Gly Asp Thr Ala Phe Thr Arg Val Leu Leu Gly Met Gly Leu 515 520 525 cgc cgt ttt tcc atg aac ccc aac aac atc ctg ccc gtc aaa aac atc 1632 Arg Arg Phe Ser Met Asn Pro Asn Asn Ile Leu Pro Val Lys Asn Ile 530 535 540 att ctg cac agc aat gtc gga cag ctc gaa agc gat att gtg aaa gtc 1680 Ile Leu His Ser Asn Val Gly Gin Leu Glu Ser Asp Ile Val Lys Val 545 550 555 560 atc cgc tgc gaa gac gaa gag aag tcg gaa aag ctg atc aaa cag atg 1728 Ile Arg Cys Glu Asp Glu Glu Lys Ser Glu Lys Leu Ile Lys Gin Met 565 570 575 aac agc gtg tet gtc gag gaa gaa gcc gac ttc aag ggg cgg aaa 1773 Asn Ser Val Ser Val GlU Glu Glu Ala Asp Phe Lys Gly Arg Lys

580 585 590 <210> 164 <211> 591 <212> PRT <213> Neisseria meningitidis <400> 164

Met Ser Ile Val Leu His Gly Val Ala Ala Gly Lys Gly Ile Ala Val 15 10 15

321

• fc ·· • fcfc · • · • • • fc fc' • • • · • ·· fcfcfcfc • • • • • • • fc • • • · • • · · • • • · • · ' · ·

WO 01/85772 PCT/GB01/02003 Gly Cys Ala His 20 Leu Ile Ala Arg Gly 25 Thr Glu GlU Val Pro 30 Gin Tyr Asp Val Ala 35 Glu Ala Asp Thr Asp 40 Ala Glu Ala Glu Arg 45 Phe Asp Ala Ala Val ⁵⁰ Lys Ala Thr Arg Lys 55 Glu Leu Glu Gin Leu 60 Arg Ser Ala Ile Pro 65 Glu Asn Ala Pro Thr 70 Glu Leu Gly Ala Phe 75 Ile Ser Leu His Leu 80 Met Leu Leu Thr Asp 85 Val Thr Leu Ser Arg 90 Glu Pro Val Asp Ue 95 Leu Arg Glu Gin Lys 100 Ue Asn Ala Glu Trp 105 Ala Leu Lys Gin Gin 110 Ser Asp Lys Leu Ala 115 Ala Gin Phe Asp Asn 120 Met Asp Asp Ala Tyr 125 Leu Arg Glu Arg Lys 130 Gin Asp Met Leu Gin 135 Val Val Arg Arg Ile 140 His Asn Asn Leu Ue 145 Gly Gin Gly Asn Glu 150 Leu Glu Val Ala Asp 155 Asn Leu Phe Asp Glu 160 Thr Val Leu Ile Ala 165 Asn Asp Leu Ser Pro 170 Ala Asp Thr Val Leu 175 Phe Lys Glu Gin Arg 180 Ile Ala Ala Phe Val 185 Thr Asp Ala Gly Gly 190 Pro Thr Gly His Thr 195 Ala Ile Leu Gly Arg 200 Ser Leu Asp Ile Pro 205 Ser Val Val Gly Leu 210 His Asn Ala Arg Lys 215 Leu Ile Thr Glu Gly 220 Glu Thr Val Ile Val 225 Asp Gly Ile Asn Gly 230' -Val Leu Ue Ile Ala 235 Pro Asp Glu Ser Val 240 Leu Asn Glu Tyr Arg 245 Arg Arg Ala Arg Glu 250 Tyr Arg Ser His Lys 255 Arg Asp Leu Asn Lys 260 Leu Lys Lys Thr Ala 265 Ala Ala Thr Ala Asp 270 Gly Val

322 ·· ·· ·« • « · · · ·

WO 01/85772 PCT/GBO1/02003 Cys Ile Glu 275 Leu Val Gly Asn Ile 280 Glu Ser Ala Glu Asp 285 Val Lys Pro Leu His 290 Asn Leu Gly Ala Asp 295 Gly Ile Gly Leu Phe 300 Arg Ser Glu Phe Leu 305 Tyr Leu Asn Arg Asp 310 Thr Met Pro Ser Glu 315 Asp Glu Gin Tyr Glu 320 Val Tyr Ser Ala Ile 325 Val Lys Lys Met Lys 330 Gly Lys Ser Val Thr 335 Ile Arg Thr Val Asp 340 Leu Gly Val Asp Lys 345 Asn Pro Arg Trp Phe 350 Gly Lys Asn Ser Thr 355 Pro Asn Gly Ser Leu 360 Asn Pro Ala Leu Gly 365 Met Thr Gly Ile Arg 370 Leu Cys Leu Ala Glu 375 Pro Val Met Phe Arg 380 Thr Gin Met Arg Ala 385 Ile Leu Arg Ala Ala 390 Ala His Gly Pro Val 395 Arg Met Met Trp Pro 400 Met Ile Thr Ser Val 405 Ser Glu Val Arg Gin 410 Cys Leu Ile His Leu 415 Asp Thr Ala Gin Arg 420 Gin Leu Ala Glu Arg 425 Gly Asp Ala Phe Gly 430 Lys Val Gly Ile Gly 435 Cys Met Ile Glu Ile 440 Pro Ser Ala Ala Leu 445 Thr Val Gly Ser Ile 450 Leu Lys Leu Val Asp 455 Phe Ile Ser Val Gly 460 Thr Asn Asp Leu Ile 465 Gin Tyr Ile Leu Ser 470 Val Asp Arg Gly Asp 475 Asp Ser Val Ser His 480 Leu Tyr Gin Pro Gly 485 His ,Pro Ala Val Leu 490 Lys Met Leu Gin His 495 Val Ile Arg Thr Ala 500 Asn Arg Met Asp Lys 505 Asp Val Ser Val Cys 510 Gly Glu Met Ala Gly 515 Asp Thr Ala Phe Thr 520 Arg Val Leu Leu Gly 525 Met Gly Leu

323 fc· ·· ·· • · · fc 8 fc • · · * • fc fc · fc • · » · • fcfcfc ···· fcfc • fc ·*·· fc

WO 01/85772

Arg Arg Phe 530

Ile Leu His 545

Ile Arg Cys

Asn Ser Val

Ser Met Asn Pro 535 Asn Asn Ile Leu Ser Asn Val 550 Gly Gin Leu Glu Ser 555 Glu Asp 565 Glu Glu Lys Ser Glu 570 Lys Ser 580 Val Glu Glu Glu Ala 585 Asp Phe

PCT/GB01/02003

Pro Val Lys Asn Ile 540

Asp Ile Val Lys Val 560

Leu Ile Lys Gin Met 575

Lys Gly Arg Lys 590 <210> 165 <211> 381 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(381) <400> 165 atg gaa cct tcc tcc tac gcg gca gaa aaa aaa gga aaa ggc ggc atc 48

Met Glu Pro Ser Ser Tyr Ala Ala Glu Lys Lys Gly Lys Gly Gly Ile

15 10 15 agg cgc gtc atc aac gca ttc ggc tat tcg ata gac ggc atc gcc gcc 96

Arg Arg Val Ile Asn Ala Phe Gly Tyr Ser Ile Asp Gly Ile Ala Ala

20 25 30 gcc tac cgt tac gaa gcg gca ttc cgt cag gtt ttg tgg ctg aac gcg 144

Ala Tyr Arg Tyr Glu Ala Ala Phe Arg Gin Val Leu Trp Leu Asn Ala

35 40 45 ctg ctg gtg tgc gcg gca ttt ttt tgg gtt tcc gaa aag tcc ctc cgc 192

Leu Leu Val cys Ala Ala Phe Phe Trp Val Ser Glu Lys Ser Leu Arg

50 55 60 ctg ccg ttg att atc gcg' tet ttt gtg tcg gtc att gtc gaa ctg ttc 240

Leu Pro Leu Ile Ile Ala Ser Phe Val Ser Val Ile Val Glu Leu Phe

65 70 75 80 aac act gcc gtc gaa gcc gcc gtc gat cat act tcg act gaa aaa cac 288

Asn Thr Ala Val Glu Ala Ala Val Asp His Thr Ser Thr Glu Lys His

85 90 95

324

WO 01/85772

PCT/GBO1/02003

gag ctg get aaa Glu Leu Ala Lys cgc gcc aaa gac gca ggt tet get gca caa ttg ttc Asp Ala Gly Ser Ala Ala Gin Leu Phe Arg Ala Lys 100 105 110 gcg atg ctg atg tta gcg gcg gtt tgg ctg tcc gcc ctg ttc ggg Ala Met Leu Met Leu Ala Ala Val Trp Leu Ser Ala Leu Phe Gly

115 120 125 <210> 166 <211> 127 <212> PRT

<213> Neisseria meningitidis <400> 166 Met 1 Glu Pro Ser Ser 5 Tyr Ala Ala Glu Lys 10 Lys Gly Lys Gly Gly 15 Ile Arg Arg Val Ile 20 Asn Ala Phe Gly Tyr 25 Ser Ile Asp Gly Ile 30 Ala Ala Ala Tyr Arg 35 Tyr Glu Ala Ala Phe 40 Arg Gin Val Leu Trp 45 Leu Asn Ala Leu Leu 50 Val Cys Ala Ala Phe 55 Phe Trp Val Ser Glu 60 Lys Ser Leu Arg Leu 65 Pro Leu Ile Ile Ala 70 Ser Phe Val Ser Val 75 Ile Val Glu Leu Phe 80 Asn Thr Ala Val Glu 85 Ala Ala Val Asp His 90 Thr Ser Thr Glu Lys 95 His Glu Leu Ala Lys 100 Arg Ala Lys Asp Ala 105 Gly Ser Ala Ala Gin 110 Leu Phe Ala Met Leu 115 Met Leu Ala Ala Val 120 Trp Leu Ser Ala Leu 125 Phe Gly

<210> 167 <211> 1257 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS

325 • · · · ·· · ···· • · · fc fc··· ·· • · · · · · ·

WO 01/85772 PCT/GB01/02003 <222> (1)..(1257, <400> 167

atg agt atg gca ctt gcc caa aaa ctt gcc gcc gac agc att gcg gcg 48 Met 1 Ser Met Ala Leu 5 Ala Gin Lys Leu Ala 10 Ala Asp Ser Ile Ala Ala 15 gtt gcc gaa gga cgc aac ctt cag gac gtg ttg gcg caa atc cgc acc 96 val Ala Glu Gly Arg Asn Leu Gin Asp Val Leu Ala Gin Ile Arg Thr 20 25 30 gcg cat ccc gac ctt acg gcg cag gaa aac ggc gcg ttg cag gac atc 144 Ala His Pro Asp Leu Thr Ala Gin Glu Asn Gly Ala Leu Gin Asp Ile 35 40 45 gcc tac ggc tgc cag cgt tat ttg ggc agt ttg aaa cat atg ctc gcg 192 Ala Tyr Gly Cys Gin Arg Tyr Leu Gly Ser Leu Lys His Met Leu Ala 50 55 60 cag atg ctg aaa aag ccg att ggc aat ccg cag ctc gaa agt ctg ctt 240 Gin Met Leu Lys Lys Pro Ile Gly Asn Pro Gin Leu Glu Ser Leu Leu 65 70 75 80 ttg gcg gcg ttg tac caa ctg cat tac acg cgc aac gcg ccg cat gct 288 Leu Ala Ala Leu Tyr Gin. Leu His Tyr Thr Arg Asn Ala Pro His Ala ⁸⁵ 90 95 gtg gtc aac gaa gct gtt gaa agc atc gcc aaa atc gga cgc ggg cag 336 Val Val Asn Glu Ala Val Glu Ser Ile Ala Lys Ile Gly Arg Gly Gin 100 105 110 tac cgt tcg ttt gcc aac gcg att ttg cgc cgc ttt ttg cgc gaa cgc 384 Tyr Arg Ser Phe Ala Asn Ala Ile Leu Arg Arg Phe Leu Arg Glu Arg 115 120 125 gac aag ctt gcg gct tcc tgc aaa aaa gac gat gtg gcg aaa cac aac 432 Asp Lys Leu Ala Ala Ser Cys Lys Lys Asp Asp Val Ala Lys His Asn 130 135 140 ctg ccg ctg tgg tgg gtg •gct tac ttg aaa aac cat tat ccg aaa cac 480 Leu Pro Leu Trp Trp Val Ala Tyr Leu Lys Asn His Tyr Pro Lys His 145 150 155 160 tgg cac aac atc gcc gcc gcg ctg caa tcc cat ccg ccg atg acg ttg 528 Trp His Asn Ile Ala Ala Ala Leu Gin Ser His Pro Pro Met Thr Leu 165 170 175 cgc gtc aac cgc ega cac ggc aac gcc gaa agc tat ttg gaa aaa ctg 576

326

• · • · • · • · • · · • * · · • · · · · · • · β • • • · · • · · · • · · · • · · · • · · · · • · · ·

WO 01/85772 PCT/GBO1/O2OO3

Arg Val Asn Arg Arg 180 His Gly Asn Ala 185 Glu Ser Tyr Leu Glu 190 Lys Leu get gcg gaa ggc att gcg gca aag gcg ttg gac gaa tat gcg gtt acg 624 Ala Ala Glu 195 Gly Ile Ala Ala Lys 200 Ala Leu Asp Glu Tyr 205 Ala Val Thr ttg gaa gaa gcc gtg ccg gtg aac cgc ctg ccc ggt ttt tea gac ggc 672 Leu Glu 210 Glu Ala Val Pro Val 215 Asn Arg Leu Pro Gly 220 Phe Sér Asp Gly att gtt tcg gta cag gac ttc ggc gcg cag cag gcg gcg tat ctc ctt 720 Xle 225 Val Ser val Gin Asp 230 Phe Gly Ala Gin Gin 235 Ala Ala Tyr Leu Leu 240 aac ccg aaa gac ggc gaa cgg att ttg gac gcg tgc gcc gcg ccg ggc 768 Asn Pro Lys Asp Gly 245 Glu Arg Ile Leu Asp 250 Ala Cys Ala Ala Pro 255 Gly ggc aag acg ggg cat atc ttg gaa ctg gcg gat tgc cgc gtt acc gcc 816 Gly Lys Thr Gly 260 His Ile Leu Glu Leu 265 Ala Asp Cys Arg Val 270 Thr Ala ttg gac att gat gca ggc cgt ctg aag cgt gtg gag gac aat atc gcc 864 Leu Asp Ile 275 Asp Ala Gly Ařg Leu 280 Lys Arg Val Glu Asp 285 Asn Ile Ala cgt ttg ggc ttt cag acg gca tcg gcg gcg tgt gcc gat gcg cgg gat 912 Arg Leu 290 Gly Phe Gin Thr Ala 295 Ser Ala Ala Cys Ala 300 Asp Ala Arg Asp ttg gcg gcg tgg tat gat gga aag cct ttc gac aca atc ctt gcc gac 960 Leu 305 Ala Ala Trp Tyr Asp 310 Gly Lys Pro Phe Asp 315 Thr Ile Leu Ala Asp 320 gtg cca tgc acc get tcc ggc gtg gcg cgg cgc aat ccc gac gtg aaa 1008 Val Pro Cys Thr Ala 325 Ser Gly Val Ala Arg 330 Arg Asn Pro Asp Val 335 Lys tgg ctg cgc cgt ccg acc gac gcg ctc aaa acc gcc cgc cag cag gaa 1056 Trp Leu Arg Arg 340 Pro Thr' Asp Ala Leu 345 Lys Thr Ala Arg Gin 350 Gin Glu gcc ctg ctg gac gca ctt tgg cag acg ctg aaa cag ggc ggc aga atg 1104 Ala Leu Leu 355 Asp Ala Leu Trp Gin 360 Thr Leu Lys Gin Gly 365 Gly Arg Met ctg ctt gcc acc tgt tcc gtg ttc gtc gaa gaa aac gac ggg caa ttg 1152

:ý

327

• · • • • • · • · • • • • · • • • • · • • · • • • • · · · • * • • » • • • • • · • · · • • · · · ·· ·· · • 0 • ·

WO 01/85772 PCT/GB01/02003

Leu Leu 370 Ala Thr Cys Ser Val Phe Val Glu Glu Asn Asp Gly Gin Leu 375 380 caa aaa ttc ctc aac cgc cat gcc gat gcc gaa ccg atc gaa tcg cgg 1200 Gin Lys Phe Leu Asn Arg His Ala Asp Ala Glu Pro Ile Glu Ser Arg 385 390 395 400 gtg ctt tta ccg aac aaa cac caa gat ggc ttt tat tac gcg ctt att 1248 Val Leu Leu Pro Asn Lys His Gin Asp Gly Phe Tyr Tyr Ala Leu Ile 405 410 415

caa aag cat 1257

Gin Lys His <210> 168 <211> 419 <212> PRT <213> Neisseria meningitidis <400> 168

Met 1 Ser Met Ala Leu 5 Ala Gin Lys Leu Ala 10 Ala Asp Ser Ile Ala 15 Ala Val Ala Glu Gly 20 Arg Asn Leu Gin Asp 25 Val Leu Ala Gin Ile 30 Arg Thr Ala His Pro 35 Asp Leu Thr Ala Gin 40 Glu Asn Gly Ala Leu 45 Gin Asp Ile Ala Tyr 50 Gly Cys Gin Arg Tyr 55 Leu Gly Ser Leu Lys 60 His Met Leu. Ala Gin 65 Met Leu Lys Lys Pro 70 Ile Gly Asn Pro Gin 75 Leu Glu Ser Leu Leu 80 Leu Ala Ala Leu Tyr 85 Gin Leu His Tyr Thr 90 Arg Asn Ala Pro His 95 Ala Val Val Asn Glu 100 Ala Val -Glu Ser Ile 105 Ala Lys Ile Gly Arg 110 Gly Gin Tyr Arg Ser 115 Phe Ala Asn Ala Ile 120 Leu Arg Arg Phe Leu 125 Arg Glu Arg Asp Lys 130 Leu Ala Ala Ser Cys 135 Lys Lys Asp Asp Val 140 Ala Lys His Asn

328

PCT/GBO1/02003

WO 01/85772

Leu Pro Leu 145 Trp Trp Val Ala Tyr Leu Lys Asn His Tyr Pro Lys His 150 155 160 Trp His Asn Ile Ala Ala Ala Leu Gin Ser His Pro Pro Met Thr Leu 165 170 175 Arg Val Asn Arg Arg His Gly Asn Ala Glu Ser Tyr Leu Glu Lys Leu 180 185 190 Ala Ala Glu Gly Ile Ala Ala Lys Ala Leu Asp Glu Tyr Ala Val Thr 195 200 205 Leu Glu Glu Ala Val Pro Val Asn Arg Leu Pro Gly Phe Ser Asp Gly 210 215 220 Ile Val Ser Val Gin Asp Phe Gly Ala Gin Gin Ala Ala Tyr Leu Leu 225 230 235 240 Asn Pro Lys Asp Gly Glu Arg Ile Leu Asp Ala Cys Ala Ala Pro Gly 245 250 255 Gly Lys Thr Gly His Ile Leu Glu Leu Ala Asp Cys Arg Val Thr Ala 260 265 270 Leu Asp Ile Asp Ala Gly Arg Leu Lys Arg Val Glu Asp Asn Ile Ala 275 280 285 Arg Leu Gly Phe Gin Thr Ala Ser Ala Ala Cys Ala Asp Ala Arg Asp 290 295 300 - Leu Ala Ala Trp Tyr Asp Gly Lys Pro Phe Asp Thr Ile Leu Ala Asp 305 310 315 320 Val Pro Cys Thr Ala Ser Gly Val Ala Arg Arg Asn Pro Asp Val Lys 325 330 335 Trp Leu Arg Arg Pro Thr Asp Ala Leu Lys Thr Ala Arg Gin Gin Glu 340 345 350 Ala Leu Leu Asp Ala Leu_xTrp Gin Thr Leu Lys Gin Gly Gly Arg Met 355 360 365 Leu Leu Ala Thr Cys Ser Val Phe Val Glu Glu Asn Asp Gly Gin Leu 370 375 380 Gin Lys Phe Leu Asn Arg His Ala Asp Ala Glu Pro Ile Glu Ser Arg

385

390

395

400

329

WO 01/85772 PCT/GB01/02003

Val Leu Leu Pro Asn Lys His Gin Asp Gly Phe Tyr Tyr Ala Leu Ile 405 410 415

Gin Lys His <210> 169 <211> 804 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1,..(804) <400> 169

atg Met 1 aat atg aaa aaa tgg att gcc gcc gcc ctt gcc tgt tcc gcg ctc 48 Asn Met Lys Lys 5 Trp Ile Ala Ala Ala 10 Leu Ala Cys Ser Ala 15 Leu gcg ctg tet gcc tgc ggc ggt cag ggt aaa gat gcc gcc gcg ccc gcc 96 Ala Leu Ser Ala Cys Gly Gly Gin Gly Lys Asp Ala Ala Ala Pro Ala 20 25 30 gca aat ccc gac aaa gtg tac cgc gtg gct tcc aac gcc gag ttt gcc 144 Ala Asn Pro Asp Lys Val Tyr Arg Val Ala Ser Asn Ala Glu Phe Ala 35 40 45 ccc ttt gaa tet tta gac tcg aaa ggc aat gtc gaa ggt ttc gat gtg 192 Pro Phe Glu Ser Leu Asp Ser Lys Gly Asn Val Glu Gly Phe Asp Val 50 55 60 gat ttg atg aac gcg atg gcg aag gcg ggc aat ttt aaa atc gaa ttc 240 Asp Leu Met Asn Ala Met Ala Lys Ala Gly Asn Phe Lys Ile Glu Phe 65 70 75 80 aaa cac cag ccg tgg gac age ctt ttc ccc gcc ttg aac aac ggc gat 288 Lys His Gin Pro Trp Asp .Ser Leu Phe Pro Ala Leu Asn Asn Gly Asp 85 90 95 gcg gac gtt gtg atg tcg ggc gta acc att acc gac gac cgc aaa cag 336 Ala Asp Val Val Met Ser Gly Val Thr Ile Thr Asp Asp Arg Lys Gin 100 105 110 tet atg gac ttc age gac ccg tat ttt gaa atc acc caa gtc gtc ctc 384 Ser Met Asp Phe ser Asp Pro Tyr Phe Glu Ile Thr Gin val val Leu

330 • ·

WO 01/85772 PCT/GB01/02003

115 120 125 gtt ccg aaa ggc aaa aaa ata tet tet tcc gaa gat ttg aaa aac atg 432 Val Pro 130 Lys Gly Lys Lys Ile 135 Ser Ser Ser Glu Asp 140 Leu Lys Asn Met aac aaa gtc ggc gtg gta acc ggc tac acg ggc gat ttc tcc gta tcc 480 Asn 145 Lys Val Gly val Val 150 Thr Gly Tyr Thr Gly Asp 155 Phe Ser Val Ser 160 aaa ctc ttg ggc aac gac aac ccg aaa atc gcg cgc ttt gaa aac gtt 528 Lys Leu Leu Gly Asn 165 Asp Asn Pro Lys Ile 170 Ala Arg Phe Glu Asn 175 Val ccc ctg att atc aaa gaa ctg gaa aac ggc ggc ttg gat tcc gtg gtc 576 Pro Leu Ile Ile 180 Lys Glu Leu Glu Asn 185 Gly Gly Leu Asp Ser 190 Val Val agc gac agc gca gtc atc gcc aat tat gtg aaa aac aat ccg acc aaa 624 Ser Asp Ser 195 Ala Val Ile Ala Asn 200 Tyr Val Lys Asn Asn 205 Pro Thr Lys ggg atg gac ttc gtt acc ctg ccc gac ttc acc acc gaa cac tac ggc 672 Gly Met 210 Asp Phe Val Thr Leu 215 Pro Asp Phe Thr Thr 220 Glu His Tyr Gly atc .gcg gta cgc aaa ggc gac gaa gca acc gtc aaa atg ctg aac gat 720 Ile 225 Ala Val Arg Lys Gly 230 Asp Glu Ala Thr Val 235 Lys Met Leu Asn Asp 240 gcg ttg aaa aaa gta cgc gaa agc ggc gaa tac gac aaa atc tac gcc 768 Ala Leu Lys Lys Val 245 Arg Glu Ser Gly Glu 250 Tyr Asp Lys Ile Tyr 255 Ala

aaa tat ttt gca aaa gaa gac gga cag gcc gca aaa 804 Lys Tyr Phe Ala Lys Glu Asp Gly Gin Ala Ala Lys 260 265

<210> 170 <211> 268 <212> PRT <213> Neisseria meningitidis <400> 170

Met Asn Met Lys Lys Trp Ile Ala Ala Ala Leu Ala Cys Ser Ala Leu 1 5 10 15

331

WO 01/85772 ·· ·· ·· • · · · · · ·

PCT/GB01/02003

Ala Leu ser Ala Cys Gly Gly Gin Gly Lys Asp Ala Ala Ala Pro Ala 20 25 30 Ala Asn Pro Asp 35 Lys Val Tyr Arg Val 40 Ala Ser Asn Ala Glu Phe Ala 45 Pro Phe Glu Ser 50 Leu Asp Ser Lys Gly 55 Asn Val Glu Gly Phe Asp Val 60 Asp 65 Leu Met Asn Ala Met Ala Lys Ala ⁷⁰ Gly Asn Phe Lys Ile Glu Phe 75 80 Lys His Gin Pro Trp Asp Ser Leu Phe 85 Pro Ala Leu Asn Asn Gly Asp 90 95 Ala Asp Val Val 100 Met Ser Gly Val Thr 105 Ile Thr Asp Asp Arg Lys^- Gin 110 Ser Met Asp Phe 115 Ser Asp Pro Tyr Phe 120 Glu Ile Thr Gin Val Val Leu 125 Val Pro Lys Gly 130 ' Lys Lys Ile Ser Ser 135 Ser Glu Asp Leu Lys Asn Met 140 Asn 145 Lys Val Gly Val Val Thr Gly Tyr 150 Thr Gly Asp Phe Ser Val-Ser 155 160 Lys Leu Leu Gly Asn Asp Asn Pro Lys 165 Ile Ala Arg Phe Glu Asn Val 170 175 Pro Leu Ile Ile 180 Lys Glu Leu Glu Asn 185 Gly Gly Leu Asp Ser Val Val 190 Ser Asp Ser Ala 195 Val Ile Ala Asn Tyr 200 Val Lys Asn Asn Pro Thr Lys 205 Gly Met Asp Phe 210 Val Thr Leu Pro Asp 215 Phe Thr Thr Glu His Tyr Gly 220 Ile 225 Ala Val Arg Lys Gly Asp Glu Ala 230' Thr Val Lys Met Leu Asn Asp 235 240 Ala Lys Leu Lys Lys Tyr Phe Ala Val Arg Glu Ser Gly 245 Lys Glu Asp Gly Gin Glu Tyr Asp Lys Ile Tyr Ala 250 255 Ala Ala Lys

260 265

332

WO 01/85772 PCT/GBO V02003 <210> 171 <211> 864 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(864) <400> 171

atg aaa gca aaa Lys atc ctg act tcc gtt gca ctg ctt gcc tgt tcc ggc Gly 48 Met 1 Lys Ala Ile Leu Thr 5 Ser Val Ala Leu 10 Leu Ala Cys Ser 15 agc ctg ttt gcc caa acg ctg gca acc gtc aac ggt cag aaa atc gac 96 Ser Leu Phe Ala Gin Thr Leu Ala Thr Val Asn Gly Gin Lys Ile Asp 20 25 30 agt tcc gtc att gat gcg cag gtt gcc gca ttc cgt gcg gaa aac agc 144 Ser Ser Val Ile Asp Ala Gin Val Ala Ala Phe Arg Ala Glu Asn Ser 35 40 45 cgt gcc gaa gac acg ccg caa ctg cgc caa tcc ctg ctg gaa aac gaa 192 Arg Ala Glu Asp Thr Pro Gin Leu Arg Gin Ser Leu Leu Glu Asn Glu 50 55 60 gtg gtc aac acc gtg gtc gca cag gaa gtg aaa cgc ctg aaa ctc gac 240 Val Val Asn Thr Val Val Ala Gin Glu Val Lys Arg Leu Lys Leu Asp 65 70 75 80 cgg tcg gca gag ttt aaa aat gcg ctt gcc aaa ttg cgt gcc gaa gcg 288 Arg Ser Ala GlU Phe Lys Asn Ala Leu Ala Lys Leu Arg Ala Glu Ala 85 90 95 aaa aag tcg ggc gac gac aag aaa ccg tcc ttc aaa acc gtt tgg cag 336 Lys Lys Ser Gly Asp Asp Lys Lys Pro Ser Phe Lys Thr Val Trp Gin 100 105 110 gcg gta aaa tat ggc ttg’ aac ggc gag gca tac gcg ctg cat atc gcc 384 Ala Val Lys Tyr Gly Leu Asn Gly Glu Ala Tyr Ala Leu His Ile Ala 115 120 125 aaa acc caa ccg gtt tcc gag cag gaa gta aaa gcc gca tat gac aat 432 Lys Thr Gin Pro Val Ser Glu Gin Glu Val Lys Ala Ala Tyr Asp Asn 130 135 140

333

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WO 01/85772

PCT/GB01/02003

atc Ile 145 agc ggt ttt Ser Gly Phe tac aaa ggt acg cag gaa gtc cag ttg ggc gaa atc 480 Tyr Lys 150 Gly Thr Gin Glu Val 155 Gin Leu Gly Glu Ile 160 ctg acc gac aag gaa gaa aat gca aaa aaa gcg gtt gcc gac ttg aag 528 Leu Thr Asp Lys Glu Glu Asn Ala Lys Lys Ala Val Ala Asp Leu Lys 165 .170 175 gcg aaa aaa ggt ttc gat gcc gtc ttg aaa caa tat tcc ctc aac gac 576 Ala Lys Lys Gly Phe Asp Ala Val Leu Lys Gin Tyr Ser Leu Asn Asp 180 185 190 cgt acc aaa cag acc ggt gcg ccg gtc gga tat gtg ccg ctg aaa gat 624 Arg Thr Lys Gin Thr Gly Ala Pro Val Gly Tyr Val Pro Leu Lys Asp 195 200 205 ttg gaa cag ggt gtt ccg ccg ctt tat cag gca att aag gac ttg aaa 672 Leu Glu Gin Gly Val Pro Pro Leu Tyr Gin Ala Ile Lys Asp Leu Lys 210 215 220 aaa ggc gaa ttt acg gca acg ccg ctg aaa aac ggc gat ttc tac ggc 720 Lys Gly Glu Phe Thr Ala Thr Pro Leu Lys Asn Gly Asp Phe Tyr Gly 225 230 235 240 gtt tat tat gtc aac gac agc cgc gag gta aaa gtg cct tet ttt gat 768 Val Tyr Tyr Val Asn Asp Ser Arg Glu Val Lys Val Pro Ser Phe Asp 245 250 255 gaa atg aaa gga cag att gcg ggc aac ctt cag gcg gaa cgg att gac 816 Glu Met Lys Gly Gin Ile Ala Gly Asn Leu Gin Ala Glu Arg Ile Asp 260 265 270 cgt gcc gtc ggt gca ctg ttg ggc aag gca aac atc aaa cct gca aaa 864 Arg Ala Val Gly Ala Leu Leu Gly Lys Ala Asn Ile Lys Pro Ala Lys 275 280 285

<210> 172 <211> 288 <212> PRT <213> Neisseria meningitidis <400> 172

Met Lys Ala Lys Ile Leu Thr Ser Val Ala Leu Leu Ala Cys Ser Gly 15 10 15

Ser Leu Phe Ala Gin Thr Leu Ala Thr Val Asn Gly Gin Lys Ile Asp 20 25 30

334 v,

WO 01/85772 PCT/GB01/02003 Ser Ser Val 35 Ile Asp Ala Gin Val 40 Ala Ala Phe Arg Ala 45 Glu Asn Ser Arg Ala 50 Glu Asp Thr Pro Gin 55 Leu Arg Gin Ser Leu 60 Leu Glu Asn Glu Val 65 Val Asn Thr val Val 70 Ala Gin Glu Val Lys 75 Arg Leu Lys Leu Asp 80 Arg Ser Ala Glu Phe 85 Lys Asn Ala Leu Ala 90 Lys Leu Arg Ala Glu 95 Ala Lys Lys Ser Gly 100 Asp Asp Lys Lys Pro 105 Ser Phe Lys Thr Val 110 Trp Gin Ala Val Lys 115 Tyr Gly Leu Asn Gly 120 Glu Ala Tyr Ala Leu 125 His Ile Ala Lys Thr 130 Gin Pro Val Ser Glu 135 Gin Glu Val Lys Ala 140 Ala Tyr Asp Asn Ile 145 Ser Gly Phe Tyr Lyš 150 Gly Thr Gin Glu Val 155 Gin Leu Gly Glu Ile 160 Leu Thr Asp Lys Glu 165 Glu Asn Ala Lys Lys 170 Ala Val Ala Asp Leu 175 Lys Ala Lys Lys Gly 180 Phe Asp Ala Val Leu 185 Lys Gin Tyr Ser Leu 190 Asn Asp Arg Thr Lys 195 Gin Thr Gly Ala Pro 200 Val Gly Tyr Val Pro 205 Leu Lys Asp Leu Glu 210 Gin Gly Val Pro Pro 215 Leu Tyr Gin Ala Ile 220 Lys Asp Leu Lys Lys 225 Gly Glu Phe Thr Ala 230 Thr Pro Leu Lys Asn 235 Gly Asp Phe Tyr Gly 240 Val Tyr Tyr Val Asn 245 Asp'Ser Arg Glu Val 250 Lys Val Pro Ser Phe 255 Asp Glu Met Lys Gly 260 Gin Ile Ala Gly Asn 265 Leu Gin Ala Glu Arg 270 Ile Asp Arg Ala Val 275 Gly Ala Leu Leu Gly 280 Lys Ala Asn Ile Lys 285 Pro Ala Lys

335 a£*

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PCT/GB01/02003 <210> 173 <211> 1794 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1794) <400> 173

ttg ccc ega att gcc atg ccc tat ttc gcc ctg ttt t gac Asp gat gcc Asp Ala 15 gta Val 48 Leu 1 Pro Arg Ile Ala 5 Met Pro Tyr Phe Ala 10 Leu Phe age ggt cgc gca aaa cgc tat caa aat cat gtg gaa age cgt ttt ttc 96 Ser Gly Arg Ala Lys Arg Tyr Gin Asn His Val Glu Ser Arg Phe Phe 20 25 30 cgt ccc gaa gaa ctc gat get ttg gac ggc gcg ctg caa tcg ggc tgg 144 Arg Pro Glu GlU Leu Asp Ala Leu Asp Gly Ala Leu Gin Ser Gly Trp 35 40 45 caa aaa ggg ctg cat gcc gtg ttg ttt gca gac tac gga ttc ggt ttg 192 Gin Lys Gly Leu His Ala Val Leu Phe Ala Asp Tyr Gly Phe Gly Leu 50 55 60 ccg ctg acg ggg gtc gag tcc gaa cgc ggc ggc aac ctt gcc ctg cac 240 Pro Leu Thr Gly Val Glu Ser Glu Arg Gly Gly Asn Leu Ala Leu His 65 70 75 80 tgg ttt gcc gac tgc gcc gac acc gat gcc gca age tgg ctt gcc ega 288 Trp Phe Ala Asp Cys Ala Asp Thr Asp Ala Ala Ser Trp Leu Ala Arg 85 90 95 cac tea gac ggc ctc ccc gcc ggc att tcc acg ccg caa tcc tcc gta 336 His Ser Asp Gly Leu Pro Ala Gly Ile Ser Thr Pro Gin Ser Ser Val 100 - 105 110 tcc gaa gcc gat tac ctc gac cat atc cgc caa atc cac gaa gcc atc 384 Ser Glu Ala Asp Tyr Leu Asp His Ile Arg Gin Ile His Glu Ala Ile 115 120 125 ega cgc ggc gac acc tat caa atc aac tac act acc cgc ctg cac ctg 432 Arg Arg Gly Asp Thr Tyr Gin Ile Asn Tyr Thr Thr Arg Leu His Leu 130 135 140

336 • 4 ·· · ·· ···· • * · · · > ·· · · « « ···· · · · • · · · · ···· · • 4 9 9 9 9 4 4 9

9444 4449 94 944 ·· ··

WO 01/85772 PCT/GBO1/02003 caa gcc tac ggc aat ccc gtc aaa ctt tac caa cgc ctg cgc cag ccc 480 Gin 145 Ala Tyr Gly Asn Pro 150 Val Lys Leu Tyr Gin 155 Arg Leu Arg Gin Pro 160 gtc ccc tat gcc gtt ttg tcc cac ctg ccc gat gca caa ggg caa tcc 528 Val Pro Tyr Ala Val 165 Leu Ser His Leu Pro 170 Asp Ala Gin Gly Gin 175 Ser gcg tgg acg ctg tgt ttc tcg ccc gaa ctc ttc ctc aaa atc ggt tcg 576 Ala Trp Thr Leu 180 Cys Phe Ser Pro Glu 185 Leu Phe Leu Lys Ile 190 Gly Ser gac ggc acc atc age acc gaa ccg atg aaa ggc acc gcg ccg att ttg 624 Asp Gly Thr 195 Ile Ser Thr Glu Pro 200 Met Lys Gly Thr Ala 205 Pro Ile Leu ggc gac gga caa gac gaa cgc cgc gcc gcc gag ttg caa gca gac ccg 672 Gly Asp 210 Gly Gin Asp Glu Arg Arg 215 Ala Ala Glu Leu 220 Gin Ala Asp Pro aaa aac cgc gcc gaa aac gtg atg att gtc gat ttg ctg cgt aac gac 720 Lys 225 Asn Arg Ala Glu Asn 230 Val Met Ile Val Asp 235 Leu Leu Arg Asn Asp 240 ctc ggc aaa atc gcc caa acc ggc aca gta tgc gta ccc gaa ccg ttt 768 Leu Gly Lys Ile Ala 245 Gin Thr Gly Thr Val 250 Cys Val Pro Glu Pro 255 Phe aaa gta tcg cgt ttc ggc age gtt tgg· cag atg acc age acc atc caa 816 Lys val Ser Arg 260 Phe Gly Ser Val Trp 265 Gin Met Thr Ser Thr 270 Ile Gin gcc caa gcc ttg ccg cac acc tcg ttc gcc gac atc ctc cgc gcc gcc 864 Ala Gin Ala 275 Leu Pro His Thr Ser 280 Phe Ala Asp Ile Leu 285 Arg Ala Ala ttc ccc tgc ggc age atc acc ggc gcg ccc aaa aaa atg agt atg cag 912 Phe Pro 290 Cys Gly Ser Ile Thr 295 Gly Ala Pro Lys Lys 300 Met Ser Met Gin att atc gaa tcg ctc gaa gcc gaa gcg cgc gga ctt tat acg ggc age 960 Ile 305 Ile Glu Ser Leu Glu 310 Ala Glu Ala Arg Gly 315 Leu Tyr Thr Gly Ser 320 atc ggc tat ttg aac ccg tgt tcc ggc ggc ttg ggg ttt gaa ggc acg 1008 Ile Gly Tyr Leu Asn 325 Pro Cys Ser Gly Gly 330 Leu Gly Phe Glu Gly 335 Thr

337

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WO 01/85772 PCT/GBO1/02003

ttc Phe aac gtc gtt atc cgc acc Thr ttg Leu tcg ctc acg ccg ctt tca gac ggc Asp Gly 1056 Asn Val Val Ile 340 Arg Ser 345 Leu Thr Pro Leu Ser 350 att tat caa ggc gtg tac ggt gtc ggt tcc ggc atc gtc atc gac agc 1104 Ile Tyr Gin Gly Val Tyr Gly Val Gly Ser Gly Ile Val Ile Asp Ser 355 360 365 gac cct gcc gcc gaa tat cgg gaa tgc ggc tgg aaa gcc cgt ttc ctc 1152 Asp Pro Ala Ala GlU Tyr Arg Glu Cys Gly Trp Lys Ala Arg Phe Leu 370 375 380 aac gaa ttg cgc CCC gac ttc ggc att ttt gaa acc ctg cgc gtg gaa 1200 Asn Glu Leu Arg Pro Asp Phe Gly Ile Phe Glu Thr Leu Arg Val Glu 385 390 395 400 aac gga cgc tgc gcc ctg ctc gac cgc cac cta tgc.cgt Ctg aaa acc 1248 Asn Gly Arg Cys Ala Leu Leu Asp Arg His Leu Cys Arg Leu Lys Thr 405 410 415 tcc gcc caa gcc ctc aac ctg ccc ctg ccc gac ggt tgc gaa aat caa 1296 Ser Ala Gin Ala Leu Asn Leu Pro Leu Pro Asp Gly Cys Glu Asn Gin 420 425 430 atc aaa caa tac atc gca cgc ttg ccc gac ggc gcg ttc cgc atc aaa 1344 Ile Lys Gin Tyr Ile Ala Arg Leu Pro Asp Gly Ala Phe Arg Ile Lys 435 440 445 gcc ctg ctc gct tca gac ggc atc agc ctg tcc cgc gcc gtt tta aac 1392 Ala Leu Leu Ala Ser Asp Gly Ile Ser Leu Ser Arg Ala Val Leu Asn 450 455 460 cgt ctg acc gac aaa caa cgc gtc atc att tcg ccg acc atc ctg ccc 1440 Arg Leu Thr Asp Lys Gin Arg Val Ile Ile Ser Pro Thr Ile Leu Pro 465 470 475 480 gca caa aac tac ctg cgc cgc ttc aaa acc acc tgc cgc acc gtc ttc 1488 Ala Gin Asn Tyr Leu Arg Arg Phe Lys Thr Thr Cys Arg Thr Val Phe 485 490 495 gac caa gcg tgg caa acc gcc gaa aca caa ggc gcg ttc gac agc ctg 1536 Asp Gin Ala Trp Gin Thr Ala Glu Thr Gin Gly Ala Phe Asp Ser Leu 500 505 510 ttt ttc aat tca gac ggc atc ctg ctc gaa ggc ggc aga agc aac gtg 1584 Phe Phe Asn Ser Asp Gly Ile Leu Leu Glu Gly Gly Arg Ser Asn Val

515 520 525

338 ·· 09 «· · ·* ···· • · · · · * ·· « · ·

4 ^Λ β 4 β — 4 · • *’«.»· » « · * · • .· 0 · · · · · · βίΐς S9SS 99 999 99 99

W0 01/85772 PCT/GB01/02003

ttc Phe gtc aaa cat cgc gga caa tgg ctc acg ccc tet tta gat tta gac 1632 Val 530 Lys His Arg Gly Gin Trp Leu 535 Thr Pro Ser 540 Leu Asp Leu Asp att tta aac ggc ata atg cgc caa gcc gtg ttg gac gaa ccg caa aaa 1680 Ile Leu Asn Gly Ile Met Arg Gin Ala Val Leu Asp Glu Pro Gin Lys 545 550 555 560 tat ttg caa aca aat caa gta atc gaa aca cac atc aca caa aaa aca 1728 Tyr Leu Gin Thr Asn Gin Val Ile Glu Thr His Ile Thr Gin Lys Thr 565 570 575 ctg caa gaa gcc gaa gaa atc cgc ctc tcc aac gcc ttg cgc ggc gta 1776 Leu Gin Glu Ala Glu Glu Ile Arg Leu Ser Asn Ala Leu Arg Gly Val 580 585 590 ttt gcc gcc gcc ctt gcc 1794 Phe Ala Ala Ala Leu Ala

595 <210> 174 <211> 598 <212> PRT <213> Neisseria meningitidis <400> 174

Leu 1 Pro Arg Ile Ala 5 Met Pro Tyr Phe Ala 10 Leu Phe Asp Asp Ala 15 Val Ser Gly Arg Ala 20 Lys Arg Tyr Gin Asn 25 His Val Glu Ser Arg 30 Phe Phe Arg Pro Glu 35 GlU Leu Asp Ala Leu 40 Asp Gly Ala Leu Gin 45 Ser Gly Trp Gin Lys Gly 50 Leu His Ala Val 55 Leu Phe Ala Asp Tyr 60 Gly Phe Gly Leu Pro 65 Leu Thr Gly Val Glu· 70 Ser Glu Arg Gly Gly 75 Asn Leu Ala Leu His 80 Trp Phe Ala Asp Cys 85 Ala Asp Thr Asp Ala 90 Ala Ser Trp Leu Ala 95 Arg His Ser Asp Gly 100 Leu Pro Ala Gly Ile 105 Ser Thr Pro Gin Ser 110 Ser Val

339 ·· ·· «« • · · · t » tt · · ·

9 9 · · · • · · · eeee eeee

WO 01/85772

9 «« «««· • · e * · » · · . · «99® 9 • · · · · eee se ee

PCT/GB01/02003

Ser Glu Ala 115 Asp Tyr Leu Asp His 120 Ile Arg Gin Ile His 125 Glu Ala Ile Arg Arg 130 Gly Asp Thr Tyr Gin 135 Ile Asn Tyr Thr Thr 140 Arg Leu His Leu Gin 145 Ala Tyr Gly Asn Pro 150 Val Lys Leu Tyr Gin 155 Arg Leu Arg Gin Pro 160 Val Pro Tyr Ala Val 165 Leu Ser His Leu Pro 170 Asp Ala Gin Gly Gin 175 Ser Ala Trp Thr Leu 180 Cys Phe Ser Pro Glu 185 Leu Phe Leu Lys Ile 190 Gly Ser Asp Gly Thr 195 Ile Ser Thr Glu Pro 200 Met Lys Gly Thr Ala 205 Pro Ile Leu Gly Asp 210 Gly Gin Asp Glu Arg Arg 215 Ala Ala Glu Leu 220 Gin Ala Asp Pro Lys 225 Asn Arg Ala Glu Asn 230 Val Met Ile Val Asp 235 Leu Leu Arg Asn Asp 240 Leu Gly Lys Ile Ala 245 Gin Thr Gly Thr Val 250 Cys Val Pro Glu Pro 255 Phe Lys Val Ser Arg 260 Phe Gly Ser Val Trp 265 Gin Met Thr Ser Thr 270 Ile Gin Ala Gin Ala 275 Leu Pro His Thr Ser 280 Phe Ala Asp Ile Leu 285 Arg Ala Ala Phe Pro 290 Cys Gly Ser Ile Thr 295 Gly Ala Pro Lys Lys 300 Met Ser Met Gin Ile 305 Ile Glu Ser Leu Glu 310 Ala Glu Ala Arg Gly 315 Leu Tyr Thr Gly Ser 320 Ile Gly Tyr Leu Asn 325 Pro' Cys Ser Gly Gly 330 Leu Gly Phe Glu Gly 335 Thr Phe Asn Val Val 340 Ile Arg Thr Leu Ser 345 Leu Thr Pro Leu Ser 350 Asp Gly Ile Tyr Gin 355 Gly Val Tyr Gly Val 360 Gly Ser Gly Ile Val 365 Ile Asp Ser

340 • ·

• · β • e í e í • · e • e e e e • ¹ β • • • β β • · · · · e e e e • · · · · e e e © e e e WO 01/85772 PCT/GB01/02003 Asp Pro Ala Ala Glu Tyr Arg Glu Cys Gly Trp Lys Ala Arg Phe Leu 370 375 380 Asn Glu Leu Arg Pro Asp Phe Gly Ile Phe Glu Thr Leu Arg Val Glu 385 390 395 400 Asn Gly Arg Cys Ala Leu Leu Asp Arg His Leu Cys Arg Leu Lys Thr 405 410 415 Ser Ala Gin Ala Leu Asn Leu Pro Leu Pro Asp Gly Cys Glu Asn Gin 420 425 430 Ile Lys Gin Tyr Ile Ala Arg Leu Pro Asp Gly Ala Phe Arg Ile Lys 435 440 445 Ala Leu Leu Ala Ser Asp Gly Ile Ser Leu Ser Arg Ala Val Leu Asn 450 455 460 Arg Leu Thr Asp Lys Gin Arg Val Ile Ile Ser Pro Thr Ile Leu Pro 465 470 475 480 Ala Gin Asn Tyr Leu Arg Arg Phe Lys Thr Thr Cys Arg Thr Val Phe 485 490 495 Asp Gin Ala Trp Gin Thr Ala Glu Thr Gin Gly Ala Phe Asp Ser Leu 500 505 510 Phe Phe Asn Ser Asp Gly Ile Leu Leu Glu Gly Gly Arg Ser Asn Val 515 520 525 Phe Val Lys His Arg Gly Gin Trp Leu Thr Pro Ser Leu Asp Leu Asp 530 535 540 Ile Leu Asn Gly Ile Met Arg Gin Ala Val Leu Asp Glu Pro Gin Lys 545 550 555 560 Tyr Leu Gin Thr Asn Gin Val ile Glu Thr His Ile Thr Gin Lys Thr 565 570 575 Leu Gin Glu Ala Glu Glu 'Ile Arg Leu Ser Asn Ala Leu Arg Gly Val 580 585 590 Phe Ala Ala Ala Leu Ala

595

341 • ·

WO 01/85772 PCT/GBO1/O2OO3 <210> 175 <211> 279 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(279) <400> 175

ttg caa Leu Gin 1 aac ttt cgg aaa ccc aat atg cag acc gtt acc atg tac aca 48 Asn Phe Arg 5 Lys Pro Asn Met Gin 10 Thr Val Thr Met Tyr 15 Thr ggt ccg ttt tgc ccc tac tgc acg atg gcg aaa agg ctg ctg cac gcg 96 Gly Pro Phe Cys Pro Tyr Cys Thr Met Ala Lys Arg Leu Leu His Ala 20 25 30 gca ggt gtc gga cat atc gac gaa atc cgt gtc gat gca agt ccc gaa 144 Ala Gly Val Gly His Ile Asp Glu Ile Arg Val Asp Ala Ser Pro Glu 35 40 45 gcc ttt gcc gaa atg cag cag ctt tcc gga cag cgc age gtg ccg cag 192 Ala Phe Ala Glu Met Gin Gin Leu Ser Gly Gin Arg Ser Val Pro Gin 50 55 60 att ttc atc ggc gaa acg cac gtc ggc gga ttt acc gac ctc tac cgc 240 Ile Phe Ile Gly Glu Thr His Val Gly Gly Phe Thr Asp Leu Tyr Arg 65 70 75 80 ctc cag cag gaa ggc ggg ctg gac gga ctg ctg aac cct .279 Leu Gin Gin Glu Gly Gly Leu Asp Gly Leu Leu Asn Pro 85 90

<210> 176 <211> 93 <212> PRT <213> Neisseria meningitidis <400> 176

Leu Gin Asn Phe Arg Lys Pro Asn Met Gin Thr Val Thr Met Tyr Thr 1 5 io 15 Gly Pro Phe Cys Pro Tyr Cys Thr Met Ala Lys Arg Leu Leu His Ala 20 25 30 Ala Gly Val Gly His Ile Asp Glu Ile Arg Val Asp Ala Ser Pro Glu

342 ·· ·· ·· · ·· ····

WO 01/85772

35 40

Ala Phe Ala Glu Met Gin Gin Leu Ser Gly Gin 50 55

Ile Phe Ile Gly Glu Thr His Val Gly Gly Phe 65 70 75

Leu Gin Gin Glu Gly Gly Leu Asp Gly Leu Leu 85 90

PCT/GB01/02003

Arg Ser Val Pro Gin 60

Thr Asp Leu Tyr Arg 80

Asn Pro <210> 177 <211> 2274 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(2274) <400> 177

atg aca Met Thr 1 aca Thr tta cat ttc tca ggc ttc ccg cgt gtc ggt gcc ttc cgc 48 Leu His Phe Ser 5 Gly Phe Pro 10 Arg Val Gly Ala Phe 15 Arg gaa ttg aaa ttc gca caa gaa aaa tac tgg cgc aaa gaa atc age gag 96 Glu Leu Lys Phe Ala Gin Glu Lys Tyr Trp Arg Lys Glu Ile Ser Glu 20 25 30 caa gaa ttg ctg get gtt get aaa gac ttg cgc gag aaa aac tgg aaa 144 Gin Glu Leu Leu Ala Val Ala Lys Asp Leu Arg Glu Lys Asn Trp Lys 35 40 45 cac cag gcc get gcc aac gcc gat tac gtt gcc gta ggc gat ttc act 192 His Gin Ala Ala Ala Asn Ala Asp Tyr Val Ala Val Gly Asp Phe Thr 50 55 60 ttc tac gac cac atc ctc gac ctg caa gtc gcc acc ggc gcg att ccc 240 Phe Tyr Asp His Ile Leu. Asp Leu Gin Val Ala Thr Gly Ala Ile Pro 65 70 75 80 gcc cgc ttc ggc ttc gac age caa aac cta tet ttg gaa caa ttc ttc 288 Ala Arg Phe Gly Phe Asp Ser Gin Asn Leu Ser Leu Glu Gin Phe Phe 85 90 95 caa ctg gcg cgc ggt aac aaa gac caa ttc get atc gaa atg acc aaa 336

343 scss íe?s ee eee ee ee

WO 01/85772 PCT/GBO1/02003 Gin Leu Ala Arg Gly Asn Lys Asp Gin Phe Ala Ile Glu Met Thr Lys 100 105 110 tgg ttc gac acc aac tac cac tac ttg gtg cct gaa ttc cac gcc gat 384 Trp Phe Asp Thr Asn Tyr His Tyr Leu val Pro Glu Phe His Ala Asp 115 120 125 acc gaa ttt aaa gcc aat gcc aaa cac tac gtt caa cag ctg caa gaa 432 Thr Glu Phe Lys Ala Asn Ala Lys His Tyr Val Gin Gin Leu Gin Glu 130 135 140 gcc caa gct ttg ggc ctg aaa gcc aaa ccg acc gtt gtg ggt ccg ttg 480 Ala Gin Ala Leu Gly Leu Lys Ala Lys Pro Thr val val Gly Pro Leu 145 150 155 160 act ttc ctg tgg gtc ggt aaa gaa aaa ggc gcc gtt gaa ttc gac cgt 528 Thr Phe Leu Trp Val Gly Lys Glu Lys Gly Ala Val Glu Phe Asp Arg 165 170 175 ctg agc ctg ttg cct aaa ctg ttg cct gtt tac gtt gaa atc ctg. act 576 Leu Ser Leu Leu Pro Lys Leu Leu Pro Val Tyr Val Glu Ile Leu Thr 180 185 190 gct ttg gtt gaa gcc ggt gcc gag tgg att caa atc gac gag cct gct 624 Ala Leu Val Glu Ala Gly Ala Glu Trp Ile Gin Ile Asp Glu Pro Ala 195 200 205 ttg act gtt gac ctg cct aaa gaa tgg gta gaa gca tac aaa gac gtt 672 Leu Thr Val Asp Leu Pro Lys Glu Trp Val Glu Ala Tyr Lys Asp. Val 210 215 220 tac gcc act ttg agc aaa gta agt gcc aaa atc ctg ctg agc act tac 720 Tyr Ala Thr Leu Ser Lys Val Ser Ala Lys Ile Leu Leu Ser Thr Tyr 225 230 235 240 ttc ggt tet gtt gcc gaa các gcc gca ttg ttg aaa tcc ctg cct gtt 768 Phe Gly Ser Val Ala Glu His Ala Ala Leu Leu Lys Ser Leu Pro Val 245 250 255 gac ggc ctg cac atc gac , ttg gta cgc gct cct gaa caa ctg gat gcg 816 Asp Gly Leu His Ile Asp Leu Val Arg Ala Pro Glu Gin Leu Asp Ala 260 265 270 ttc gcc gac tac gac aaa gtt ctg tet gcc ggc gtc att gac ggc cgc 864 Phe Ala Asp Tyr Asp Lys Val Leu Ser Ala Gly Val Ile Asp Gly Arg 275 280 285 aat att tgg cgc gcc aac ctg aac aaa gtt ttg gaa act gtt gag ctt 912

344 ses e e tese ssec st

PCT/GB01/02003

WO 01/85772 Asn Ile 290 Trp Arg Ala Asn Leu 295 Asn Lys ctg caa gcc aaa ctg ggc gac cgt ttg Leu 305 Gin Ala Lys Leu Gly 310 Asp Arg Leu ctg ctg cac act ccg ttt gac ttg tca Leu Leu His Thr Pro 325 Phe Asp Leu Ser aac aaa ccc gac ctg tac tet tgg ttg Asn Lys Pro Asp 340 Leu Tyr Ser Trp Leu 345 caa gaa ttg cgc gtc ctg aaa gcc gca Gin Glu Leu 355 Arg Val Leu Lys Ala 360 Ala gtt gcc gaa gaa ctg gct gcc agc caa Val Ala 370 Glu Glu Leu Ala Ala 375 Ser Gin aac agc agc gaa atc cac cgt gca gac Asn 395 Ser Ser Glu Ile His 390 Arg Ala Asp ttg cct gcc aac gca gac caa cgc aaa Leu Pro Ala Asn Ala 405 Asp Gin Arg Lys aaa gcg caa caa gca tgg ttg aac ctg Lys Ala Gin Gin 420 Ala Trp Leu Asn Leu 425 atc ggt tet ttc ccg caa act acc gaa Ile Gly Ser 435 Phe Pro Gin Thr Thr 440 Glu ttc aaa aaa ggc gaa ctg 'tet gcc gcc Phe Lys 450 Lys Gly Glu Leu Ser 455 Ala Ala aaa gaa atc gcc ttg gtg gtt gaa gag Lys 465 Glu Ile Ala Leu Val 470 Val Glu Glu gta ctg gta cac ggc gaa gcc gag cgt

Val Leu Glu 300 Thr Val Glu Leu tgg att tcc agc tet tgc tcg 960 Trp Ile 315 Ser Ser Ser Cys Ser 320 gtt gaa gaa aaa ctg aaa gcc 1008 Val 330 Glu Glu Lys Leu Lys 335 Ala gca ttc acc ctg caa aaa acc 1056 Ala Phe Thr Leu Gin 350 Lys Thr ttg aac gaa ggc cgt gat tet 1104 Leu Asn Glu Gly 365 Arg Asp Ser gct gct gcc gac tcc cgt gcc 1152 Ala Ala Ala 380 Asp Ser Arg Ala gtt gcc aaa cgc ctg gcc gat 1200 Val Ala 395 Lys Arg Leu Ala Asp 400 tcc cca ttt gcc gac cgt atc 1248 Ser 410 Pro Phe Ala Asp Arg 415 Ile cct ctg ctg ccg acg acc aac 1296 Pro Leu Leu Pro Thr 430 Thr Asn atc cgc caa gca cgc gca gcc 1344 Ile Arg Gin Ala 445 Arg Ala Ala gat tac gaa gcc gcg atg aaa 1392 Asp Tyr Glu 460 Ala Ala Met Lys caa gaa aaa ctg gac ttg gac 1440 Gin Glu 475 Lys Leu Asp Leu Asp 480 aac gac atg gtt gaa tac ttc 1488

345

9999 «««s 99 999 99 99

WO 01/85772 PCT/GB01/02003

Val Leu Val His Gly 485 Glu Ala Glu Arg Asn 490 Asp Met Val Glu Tyr 495 Phe ggc gaa ttg ttg age ggt ttt gca ttc acc caa tat ggc tgg gta caa 1536 Gly Glu Leu Leu 500 Ser Gly Phe Ala Phe 505 Thr Gin Tyr Gly Trp 510 Val Gin age tac ggt tet cgc tgc gtt aaa cca ccg att atc ttc ggt gac gta 1584 Ser Tyr Gly 515 Ser Arg Cys Val Lys 520 Pro Pro Ile Ile Phe 525 Gly Asp Val age cgt cct gaa gcc atg acc gta get tgg tet act tac gca caa age 1632 Ser Arg 530 Pro Glu Ala Met Thr 535 Val Ala Trp Ser Thr 540 Tyr Ala Gin Ser ctg acc aaa cgc ccg atg aaa ggt atg ttg acc ggc cct gta acc att 1680 Leu 545 Thr Lys Arg Pro Met 550 Lys Gly Met Leu Thr 555 Gly Pro Val Thr Ile 560 ctg caa tgg tet ttc gtc cgc aac gat att cct cgc tet acc gtg tgt 1728 Leu Gin Trp Ser Phe 565 Val Arg Asn Asp Ile 570 Pro Arg Ser Thr Val 575 Cys aaa caa atc gca ctg get ctg aac gac gaa gta ttg gat ctg gaa aaa 1776 Lys Gin Xle Ala 580 Leu Ala Leu Asn Asp 585 Glu Val Leu Asp Leu 590 Glu Lys gca ggc atc aaa gtc atc caa atc gac gaa cct gcc atc cgc gaa ggc 1824 Ala Gly Ile 595 Lys Val Ile Gin Ile 600 Asp Glu Pro Ala Ile 605 Arg Glu Gly Ctg cct ttg aaa cgt gcc gat tgg gat gcc tac ctg aac tgg gcc ggc 1872 Leu Pro 610 Leu Lys Arg Ala Asp 615 Trp Asp Ala Tyr Leu 620 Asn Trp Ala Gly gaa tet ttc cgc ctg tcc tet acc ggt tgc gaa gac age act caa atc 1920 Glu 625 Ser Phe Arg Leu Ser 630 Ser Thr Gly Cys Glu 635 Asp Ser Thr Gin Ile 640 cac acc cat atg tgc tac 'tet gag ttc aac gac atc ctg cct gcg att 1968 His Thr His Met Cys 645 Tyr Ser Glu Phe Asn 650 Asp Ile Leu Pro Ala 655 Ile get gca atg gat gcg gac gtg atc acc atc gag act tea cgt tcc gac 2016 Ala Ala Met Asp 660 Ala Asp Val Ile Thr 665 Ile Glu Thr Ser Arg 670 Ser Asp atg gaa ctc ttg acc gcg ttc ggc gaa ttc aaa tac ccg aac gac atc 2064

346 φφ • · · · φφ • · φφφφ φ φφφφ φφφφ φ φ φ φφφ φφφφ φφφφ φφφφ φφ φφφ φφ φφ

WO 01/85772

PCT/GBO1/02003

Met Glu Leu Leu Thr Ala Phe Gly Glu 675 680 ggc ccg ggg gtt tac gac atc cac age Gly Pro Gly Val Tyr Asp Ile His Ser 690 695 gaa gtg gag cac ctg ttg cgc aaa gcc Glu Val Glu His Leu Leu Arg Lys Ala 705 710 cgt ctg tgg gtt aac ccg gac tgc ggc Arg Leu Trp Val Asn Pro Asp cys Gly 725 gaa act ctg gaa caa ctc caa gtg atg Glu Thr Leu Glu Gin Leu Gin Val Met 740 745 cgt gcc gaa ttg gcg aaa Arg Ala Glu Leu Ala Lys 755

Phe Lys Tyr Pro 685 Asn Asp Ile ccg cgc gta ccg aca gaa gcc 2112 Pro Arg Val 700 Pro Thr Glu Ala atc gag gtt gta ccg gtt gaa 2160 Ile Glu 715 Val Val Pro Val Glu 720 ctg aaa aca cgc ggc tgg aaa 2208 Leu 730 Lys Thr Arg Gly Trp 735 Lys atg aac gta acc cac aaa ttg 2256 Met Asn Val Thr His Lys Leu

750

2274 <210> 178 <211> 758 <212> PRT <213> Neisseria meningitidis <400> 178

Met 1 Thr Thr Leu His 5 Phe Ser Gly Phe Pro 10 Arg Val Gly Ala Phe 15 Arg Glu Leu Lys Phe 20 Ala Gin Glu Lys Tyr Trp Arg 25 Lys Glu Ile 30 Ser Glu Gin Glu Leu 35 Leu Ala Val Ala Lys 40 Asp Leu Arg Glu Lys Asn 45 Trp Lys His Gin 50 Ala Ala Ala Asn Ala 55 Asp Tyr Val Ala Val 60 Gly Asp Phe Thr Phe 65 Tyr Asp His Ile Leu Asp 70 Leu Gin Val Ala 75 Thr Gly Ala Ile Pro 80 Ala Arg Phe Gly Phe Asp Ser Gin Asn Leu Ser Leu Glu Gin Phe Phe

85 90 95

347 ·· ·· ·* • · ·*· · • ···· · · · e · • · ··· · · · · e;*e«s$e » * · t« ·« · ·

WO 01/85772 PCT/GB01/02003

Gin Leu Ala Arg 100 Gly Asn Lys Asp Gin 105 Phe Ala Ile Glu Met 110 Thr Lys Trp Phe Asp 115 Thr Asn Tyr His Tyr 120 Leu Val Pro Glu Phe 125 His Ala Asp Thr Glu 130 Phe Lys Ala Asn Ala 135 Lys His Tyr Val Gin 140 Gin Leu Gin Glu Ala 145 Gin Ala Leu Gly Leu 150 Lys Ala Lys Pro Thr 155 Val Val Gly Pro Leu 160 Thr Phe Leu Trp Val 165 Gly Lys Glu Lys Gly 170 Ala Val Glu Phe Asp 175 Arg Leu Ser Leu Leu 180 Pro Lys Leu Leu Pro 185 Val Tyr Val Glu Ile 190 Leu Thr Ala Leu Val 195 Glu Ala Gly Ala Glu 200 Trp Ile Gin Ile Asp 205 Glu Pro Ala Leu Thr 210 Val Asp Leu Pro Lys 215 Glu Trp Val Glu Ala 220 Tyr Lys Asp Val Tyr 225 Ala Thr Leu Ser Lys 230 Val Ser Ala Lys Ile 235 Leu Leu Ser Thr Tyr 240 Phe Gly Ser Val Ala 245 Glu His Ala Ala Leu 250 Leu Lys Ser Leu Pro 255 val Asp Gly Leu His 260 Ile Asp Leu Val Arg 265 Ala Pro Glu Gin Leu 270 Asp Ala Phe Ala Asp 275 Tyr Asp Lys Val Leu 280 Ser Ala Gly Val Ile 285 Asp Gly Arg Asn Ile 290 Trp Arg Ala Asn Leu 295 Asn Lys Val Leu Glu 300 Thr Val Glu Leu Leu 305 Gin Ala Lys Leu Gly 310 Asp Arg Leu Trp Ile 315 Ser Ser Ser Cys Ser 320 Leu Leu His Thr Pro 325 Phe Asp Leu Ser Val 330 Glu Glu Lys Leu Lys 335 Ala Asn Lys Pro Asp 340 Leu Tyr Ser Trp Leu 345 Ala Phe Thr Leu Gin 350 Lys Thr

348 • · • · ··· · · · · ··«···'·« · · toto* toto 9 9

WO 01/85772 PCT/GB01/02003

Gin Glu Leu 355 Arg Val Leu Lya Ala 360 Ala Leu Asn Glu Gly Arg 365 Asp Ser Val Ala 370 Glu Glu Leu Ala Ala 375 Ser Gin Ala Ala Ala 380 Asp Ser Arg Ala Asn 385 Ser Ser Glu Ile His 390 Arg Ala Asp Val Ala 395 Lys Arg Leu Ala Asp 400 Leu Pro Ala Asn Ala 405 Asp Gin Arg Lys Ser 410 Pro Phe Ala Asp Arg 415 Ile Lys Ala Gin Gin 420 Ala Trp Leu Asn Leu 425 Pro Leu Leu Pro Thr 430 Thr Asn Ile Gly Ser 435 Phe Pro Gin Thr Thr 440 Glu Ile Arg Gin Ala 445 Arg Ala Ala Phe Lys 450 Lys Gly Glu Leu Ser 455 Ala Ala Asp Tyr Glu 460 Ala Ala Met Lys Lys 465 Glu Ile Ala Leu Val 470 Val Glu Glu Gin Glu 475 Lys Leu Asp Leu Asp 480 Val Leu Val His Gly 485 Glu Ala Glu Arg Asn 490 Asp Met Val Glu Tyr 495 Phe Gly Glu Leu Leu 500 Ser Gly Phe Ala Phe 505 Thr Gin Tyr Gly Trp 510 Val Gin Ser Tyr Gly 515 Ser Arg cys Val Lys 520 Pro Pro Ile Ile Phe 525 Gly Asp Val Ser Arg 530 Pro Glu Ala Met Thr 535 Val Ala Trp Ser Thr 540 Tyr Ala Gin Ser Leu 545 Thr Lys Arg Pro Met 550 Lys Gly Met Leu Thr 555 Gly Pro Val Thr Ile 560 Leu Gin Trp Ser Phe Val Arg Asn Asp Ile Pro Arg Ser Thr Val Cys

565 570 575 Lys Gin Ile Ala Leu Ala Leu Asn Asp Glu Val Leu Asp Leu Glu Lys 580 585 590 Ala Gly Ile Lys Val Ile Gin Ile Asp Glu Pro Ala Ile Arg Glu Gly 595 600 605

349

WO Ot/85772 PCT/GBO1/O2OO3

Leu Pro Leu Lys Arg Ala Asp Trp Asp Ala Tyr Leu Asn Trp Ala Gly 610 615 620 Glu Ser Phe Arg Leu Ser Ser Thr Gly Cys Glu Asp Ser Thr Gin Ile 625 630 635 640 His Thr His Met Cys Tyr Ser Glu Phe Asn Asp Ile Leu Pro Ala Ile 645 650 655 Ala Ala Met Asp Ala Asp Val Ile Thr Ile Glu Thr Ser Arg Ser Asp 660 665 670 Met Glu Leu Leu Thr Ala Phe Gly Glu Phe Lys Tyr Pro Asn Asp Ile 675 680 685 Gly Pro Gly Val Tyr Asp Ile His Ser Pro Arg Val Pro Thr Glu Ala 690 695 700 Glu Val GlU His Leu Leu Arg Lys Ala Ile Glu Val Val Pro Val Glu 705 710 715 720 Arg Leu Trp Val Asn Pro Asp Cys Gly Leu Lys Thr Arg Gly Trp Lys 725 730 735 Glu Thr Leu Glu Gin Leu Gin Val Met Met Asn Val Thr His Lys Leu 740 745 750 Arg Ala Glu Leu Ala Lys

755 <210> 179 <211> 567 <212> DNA <213> Neisseria meningitidis <220>

<221> <222> CDS (1) .. (867, <400> 179 atg aaa ccc ata cgg aaa gcc gtc ttc ccc gtc gca ggg atg gga aca 48 Met Lys Pro Ile Arg Lys Ala Val Phe Pro Val Ala Gly Met Gly Thr 1 5 10 15 cgc ttc ctg ccc gcc acc aag gca agc ccg aaa gaa atg ctg ccc atc 96 Arg Phe Leu Pro Ala Thr Lys Ala Ser Pro Lys Glu Met Leu Pro Ile

350 • · 9 9 9 9 · β β β 9 9 9 9

9 · 9 99 · 9 ··

W0 01/85772

PCT/GBO1/02003

gtc gac aag Val Asp Lys 35 ccg Pro ctg Leu atc caa tac gcc gta gaa gaa gcc gtg gaa gcc 144 Ile Gin Tyr 40 Ala Val Glu Glu Ala Val 45 Glu Ala ggc tgc acg gaa atg gtg ttt gtt acc gga cgc aac aaa cgc age atc 192 Gly Cys Thr Glu Met Val Phe Val Thr Gly Arg Asn Lys Arg Ser Tle 50 55 60 gaa gac cat ttc gac aag gca tac gaa ctc gaa acc gag ttg gaa atg 240 Glu Asp His Phe Asp Lys Ala Tyr Glu Leu Glu Thr Glu Leu Glu Met 65 70 75 80 cgc cat aaa gac aaa ttg ttg gaa cac gtc cgc aac atc ctg ccg ccg 288 Arg His Lys Asp Lys Leu Leu Glu His Val Arg Asn Ile Leu Pro Pro 85 90 95 aac att acc tgc ctc tac atc cgt cag gcg gaa gca ctg ggc ttg gga 336 Asn Ile Thr Cys Leu Tyr Ile Arg Gin Ala Glu Ala Leu Gly Leu Gly 100 105 110 cac gcc gtc ttg tgc gcc cgc gcc gcc atc ggc gac gaa ccc ttt gcc 384 His Ala Val Leu Cys Ala Arg Ala Ala Ile Gly Asp Glu Pro Phe Ala 115 120 125 gtg att ttg gct gac gat tta atc gat gcc caa aaa ggc gcg ctc aaa 432 Val Ile Leu Ala Asp Asp Leu Ile Asp Ala Gin Lys Gly Ala Leu Lys 130 135 140 caa atg gtc gaa gtg tac gaa cgc age ggc aac age att ťtg ggc gta 480 Gin Met Val Glu Val Tyr Glu Arg Ser Gly Asn Ser Ile Leu Gly Val 145 150 155 160 gaa act gtc gaa ccg tcg caa acc ggc tca tac ggc atc gtc gaa acc 528 Glu Thr Val Glu Pro Ser Gin Thr Gly Ser Tyr Gly Ile Val Glu Thr 165 170 175 gaa cag ctc aaa cag ttc caa cgc att acc ggc att gtc gaa aaa ccc 576 Glu Gin Leu Lys Gin Phe Gin Arg Ile Thr Gly Ile Val Glu Lys Pro 180 185 190 aag ccc gaa gac gcg ccc tcc aac ctt gcc gtc gtc gga cgc tac atc 624 Lys Pro Glu Asp Ala Pro Ser Asn Leu Ala Val Val Gly Arg Tyr Ile 195 200 205 ctt acc ccg cgc att ttc gac tta ctg acc gga ctg ccg cgc ggc gcg 672 Leu Thr Pro Arg Ile Phe Asp Leu Leu Thr Gly Leu Pro Arg Gly Ala

351 • ·

WO 01/85772

PCT/GBO1/02003

210

220

215

ggc aac gaa atc cag ctt aca gac Gly 225 Asn Glu Ile Gin Leu 230 Thr Asp gaa ttt gtc cta gcg cac ccč ttt Glu Phe Val Leu Ala 245 His Pro Phe age aaa ctg ggc tat ctg gaa gcc Ser Lyš Leu Gly 260 Tyr Leu Glu Ala ccc gaa acc ggc gaa ccc ttc cgc Pro Glu Thr 275 Gly Glu Pro Phe Arg 280

ggc Gly atc gcc aag ctg ctc gat cac Ile Ala Lys Leu Leu Asp His 720 235 240 gaa ggc acg cgc tac gac tgc ggc 768 Glu Gly Thr Arg Tyr Asp Cys Gly 250 255 acc gtc gcc tac ggc ctg aaa cac 816 Thr Val Ala Tyr Gly Leu Lys His 265 270 cgc ctt ttg gaa aaa tac cgt acc 864 Arg Leu Leu Glu Lys Tyr Arg Thr

285 gaa 867

Glu <210> 180 <211> 289 <212> PRT

<213> Neisseria meningitidis <400> 180 Met 1 Lys Pro Ile Arg 5 Lys Ala Val Phe Pro 10 Val Ala Gly Met Gly 15 Thr Arg Phe Leu Pro 20 Ala Thr Lys Ala Ser 25 Pro Lys Glu Met Leu 30 Pro Ile Val Asp Lys 35 Pro Leu Ile Gin Tyr 40 Ala Val Glu Glu Ala 45 Val Glu Ala Gly Cys 50 Thr Glu Met Val Phe 55 Val Thr Gly Arg Asn 60 Lys Arg Ser Ile Glu 65 Asp His Phe Asp Lys 70 Ala Tyr Glu Leu Glu 75 Thr Glu Leu Glu Met 80 Arg His Lys Asp Lys 85 Leu Leu Glu His Val 90 Arg Asn Ile Leu Pro 95 Pro Asn Ile Thr Cys 100 Leu Tyr Ile Arg Gin 105 Ala Glu Ala Leu Gly 110 Leu Gly

352

WO 01/85772 PCT/GBO1/02003 His Ala Val Leu Cys 115 Ala Arg Ala Ala Ile Gly Asp Glu Pro Phe Ala 120 125 Val Ile Leu Ala Asp ¹³⁰ Asp Leu Ile Asp Ala Gin Lys Gly Ala Leu Lys 135 140 Gin Met Val Glu Val 145 Tyr Glu Arg Ser Gly Asn Ser Ile Leu Gly Val 150 155 160 Glu Thr Val Glu Pro 165 Ser Gin Thr Gly Ser Tyr Gly Ile Val Glu Thr 170 175 Glu Gin Leu Lys Gin 180 Phe Gin Arg Ile Thr Gly Ile Val Glu Lys Pro 185 190 Lys Pro Glu Asp Ala 195 Pro Ser Asn Leu Ala Val Val Gly Arg Tyr Ile 200 205 Leu Thr Pro Arg Ile 210 Phe Asp Leu Leu Thr Gly Leu Pro Arg Gly Ala 215 220 Gly Asn Glu Ile Gin 225 Leu Thr Asp Gly Ile Ala Lys Leu Leu Asp His 230 235 240 Glu Phe Val Leu Ala 245 His Pro Phe Glu Gly Thr Arg Tyr Asp Cys Gly 250 255 Ser Lys Leu Gly Tyr 260 Leu Glu Ala Thr Val Ala Tyr Gly Leu Lys His 265 270 Pro Glu Thr Gly Glu 275 Pro Phe Arg Arg Leu Leu Glu Lys Tyr Arg Thr 280 285

GlU <210> 181 <211> 603 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(603)

353 • · ·

WO 01/85772 <400> 181

PCT/GBO1/02003

atg aca ata atg aat atc aat act tcc Met 1 Thr Xle Met Asn 5 Ue Asn Thr Ser gaa cac acc gga caa tgg ttg gaa aaa Glu His Thr Gly 20 Gin Trp Leu Glu Lys 25 tgt ccc ttt gcc aaa gcc ccc cac gtt Cys Pro Phe 35 Ala Lys Ala Pro His 40 Val atc agc gaa gcc aaa cac ctt gac agt Xle Ser 50 Glu Ala Lys His Leu 55 Asp Ser gaa ctg cag ega ctg ggc aat aca ccc Glu 65 Leu Gin Arg Leu Gly 70 Asn Thr Pro ctg ctg gtt cac ccg acc cta ttc ccc Leu Leu Val His Pro 85 Thr Leu Phe Pro atg ctc gac att gcc gat gcc gcc gtt Met Leu Asp Ile 100 Ala Asp Ala Ala Val 105 atc atc caa atc gcc ccg ttt cat ccc Xle Xle Gin 115 Ile Ala Pro Phe His 120 Pro gat tca gac gac atc ggc aac tac acc Asp Ser 130 Asp Asp Ile Gly Asn 135 Tyr Thr ctg cac ctc atc cgc gaa gac agc att Leu 145 His Leu Ile Arg Glu 150 Asp Ser Ile ccc gac gct tcg gca ata ttc gaa cgc Pro Asp Ala Ser Ala 165 Ile Phe Glu Arg atg gga cat gag ggc tgg gca aaa ctc Met Gly His Glu 180 Gly Trp Ala Lys Leu 185

gaa aat aaa gat gct gtt gcc 48 Glu 10 Asn Lys Asp Ala Val 15 Ala gcc gtc atc ggt ctg aac ctg 96 Ala Val Ile Gly Leu 30 Asn Leu aaa aac ctt gtc cgc atc gca 144 Lys Asn Leu Val 45 Arg Ile Ala ttt ttg gaa gac ttg gac gaa 192 Phe Leu Glu 60 Asp Leu Asp Glu gcc acc gaa ctg gaa act acc 240 Ala Thr 75 Glu Leu Glu Thr Thr 80 gat ttc gac gta ttc aac gat 288 Asp 90 Phe Asp Val Phe Asn 95 Asp gtc gaa aac ggc ttg gaa ggc 336 Val Glu Asn Gly Leu 110 Glu Gly gat ttc caa ttt gaa ggc acg 384 Asp Phe Gin Phe 125 Glu Gly Thr aac cgt tet ccc tat ccg acg 432 Asn Arg Ser 140 Pro Tyr Pro Thr gcc aaa gcc gca caa gcc ttt 480 Ala Lys 155 Ala Ala Gin Ala Phe 160 aat atc gcc ctg ctg gaa aaa 528 Asn 170 Ile Ala Leu Leu Glu 175 Lys ggt atc aca tcc tgc cct tat 576 Gly Ile Thr Ser Cys Pro Tyr

190

354 ·*

WO 01/85772 PCT/GB01/02003 ccg cac aat aag aaa aat att tea aaa 603

Pro His Asn Lys Lys Asn Ile Ser Lys

195 200 <210> 182 <211> 201 <212> PRT <213> Neisseria meningitidis <400> 182

Met 1 Thr Ile Met Asn 5 Ile Asn ,Thr Ser Glu 10 Asn Lys Asp Ala Val 15 Ala Glu His Thr Gly 20 Gin Trp Leu Glu Lýs 25 Ala Val Ile Gly Leu 30 Asn Leu Cys Pro Phe 35 Ala Lys Ala Pro His 40 Val Lys Asn Leu Val 45 Arg Ile Ala Ile Ser 50 Glu Ala Lys His Leu 55 Asp Ser Phe Leu Glu 60 Asp Leu Asp Glu Glu 65 Leu Gin Arg Leu Gly 70 Asn Thr Pro Ala Thr 75 Glu Leu Glu Thr Thr 80 Leu Leu Val His Pro 85 Thr Leu Phe Pro Asp 90 Phe Asp Val Phe Asn 95 Asp Met Leu Asp Ile 100 Ala Asp Ala Ala Val 105 Val Glu Asn Gly Leu 110 Glu Gly Ile Ile Gin 115 Ile Ala Pro Phe His 120 Pro Asp Phe Gin Phe 125 Glu Gly Thr Asp Ser 130 Asp Asp Ile Gly Asn 135 Tyr Thr Asn Arg Ser 140 Pro Tyr Pro Thr Leu 145 His Leu Ile Arg Glu 150 Asp Ser Ile Ala Lys 155 Ala Ala Gin Ala Phe 160 Pro Asp Ala Ser Ala 165 Ile Phe Glu Arg Asn 170 Ile Ala Leu Leu Glu 175 Lys Met Gly His Glu Gly Trp Ala Lys Leu Gly Ile Thr Ser Cys Pro Tyr

180 185 190

355 ssec β e c «

WO 01/85772 PCT/GB01/02003

Pro His Asn Lys Lys Asn Ile Ser Lys 195 200 <210> 183 <211> 1338 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1338) <400> 183

ttg Leu 1 cac acc ctt Leu cta gct ttt atc ttc gcc atc ctg att ttg gtc Leu Val 15 age Ser 48 His Thr Leu Ala 5 Phe Ile Phe Ala 10 Ile Leu Ile ctg cac gaa ttc gga cac tac atc gtc gcc aga ttg tgc ggc gtc aag 96 Leu His Glu Phe Gly His Tyr Ile Val Ala Arg Leu Cys Gly Val Lys 20 25 30 gtt gtg cgt ttt tcc gtc ggc ttc ggc aaa ccg ttt ttc acc ega aag 144 Val Val Arg Phe Ser Val Gly Phe Gly Lys Pro Phe Phe Thr Arg Lys 35 40 45 cgc ggc gac acc gaa tgg tgc ctc gcc ccg att ccg ttg ggc ggt tac 192 Arg Gly Asp Thr Glu Trp Cys Leu Ala Pro Ile Pro Leu Gly Gly Tyr 50 55 60 gtc aaa atg gtc gac acg cgc gaa ggc gaa gta tea gaa gcc gat tta 240 Val Lys Met Val Asp Thr Arg Glu Gly Glu Val Ser Glu Ala Asp Leu 65 70 75 80 ccc tac gct ttt gac aaa caa cac ccc gcc aag cgc atc gcc atc gtc 288 Pro Tyr Ala Phe Asp Lys Gin His Pro Ala Lys Arg Ile Ala Ile Val 85 90 95 gcc gcc ggc ccg ctg acc. -aac ctc gca ctg gcg gtt ttg ctg tac gga 336 Ala Ala Gly Pro Leu Thr Asn Leu Ala Leu Ala Val Leu Leu Tyr Gly 100 105 110 ctg age ttt tcc ttc ggc gtt acc gaa ctg cgc ccc tat gtc ggc aca 384 Leu Ser Phe Ser Phe Gly Val Thr Glu Leu Arg Pro Tyr Val Gly Thr 115 120 125 gtc gaa ccc gac acc att gcc gcc cgc gcc ggc ttc caa age ggc gac 432

356

49 94 eceeeeee «β

WO 01/85772 PCT/GB01/02003

Val Glu 130 Pro Asp Thr Ile Ala 135 Ala Arg Ala Gly Phe 140 Gin Ser Gly Asp aaa ata caa tcc gtc aac ggc aca ccc gtt gca gat tgg ggc agc gcg 480 Lys 145 Ile Gin Ser Val Asn 150 Gly Thr Pro Val Ala 155 Asp Trp Gly Ser Ala 160 caa acc gaa atc gtc ctc aac ctc gaa gcc ggc aaa gtc gcc gtc ggc 528 Gin Thr Glu Xle Val 165 Leu Asn Leu Glu Ala 170 Gly Lys Val Ala Val 175 Gly gtt cag acg gca tcg ggc gcg caa acc gtc cgc acc atc gat gcc gca 576 Val Gin Thr Ala 180 Ser Gly Ala Gin Thr 185 Val Arg Thr Ile Asp 190 Ala Ala ggc acg ccg gaa gcc ggt aaa atc gca aaa aac caa ggc tac atc gga 624 Gly Thr Pro 195 Glu Ala Gly Lys Ile 200 Ala Lys Asn Gin Gly 205 Tyr Ile Gly ctg atg ccc ttt aaa atc aca acc gtt gcc ggc ggc gtg gaa aaa ggc 672 Leu Met 210 Pro Phe Lys Ile Thr 215 Thr Val Ala Gly Gly 220 Val Glu Lys Gly agc ccc gcc gaa aaa gca ggc ctg aaa ccg ggc gac agg ctg act gcc 720 Ser 225 Pro Ala Glu Lys Ala 230 Gly Leu Lys Pro Gly Asp 235 Arg Leu Thr Ala 240 gcc gac ggc aaa ccc atc gcc tea tgg caa gaa tgg gca aac ctg acc 768 Ala Asp Gly Lys Pro 245 Ile Ala Ser Trp Gin 250 Glu Trp Ala Asn Leu 255 Thr cgc caa agc ccc ggc aaa aaa atc acc ctg acc tac gaa cgc gcc gga 816 Arg Gin Ser Pro 260 Gly Lys Lys Ile Thr 265 Leu Thr Tyr Glu Arg 270 Ala Gly caa acc cat acc gcc gac atc cgc ccc gat act gtc gaa cag ccc gac 864 Gin Thr His 275 Thr Ala Asp Ile Arg 280 Pro Asp Thr Val Glu 285 Gin Pro Asp cac acc ctg atc ggg cgc gtc ggc ctc cgt ccg cag ccg gac agg gcg 912 His Thr 290 Leu Ile Gly Arg'Val 295 Gly Leu Arg Pro Gin 300 Pro Asp Arg Ala tgg gac gcg caa atc cgc cgc agc tac cgt ccg tet gtt gtc cgc gca 960 Trp 305 Asp Ala Gin Ile Arg 310 Arg Ser Tyr Arg Pro 315 Ser Val Val Arg Ala 320 ttc ggc atg ggc tgg gaa aaa acc gtt tcc cac tcg tgg aca acc ctc 1008

357

WO 01/85772 PCT/GBO1/02003 Phe Gly Met Gly Trp Glu Lys Thr Val Ser His Ser Trp Thr Thr Leu 325 330 335 aaa ttt ttc ggc aaa cta atc age ggc aac gcc tcc gtc age cat att 1056 Lys Phe Phe Gly Lys Leu Ile Ser Gly. Asn Ala Ser Val Ser His Ile 340 345 350 tcc ggt ccg ctg acc att gcc gat att gcc gga cag tcc gcc gaa ctc 1104 Ser Gly Pro Leu Thr Ile Ala Asp Ile Ala Gly Gin Ser Ala Glu Leu 355 360 365 ggc ttg caa agt tat ttg gaa ttt ttg gca ctg gtc age atc age ctc 1152 Gly Leu Gin Ser Tyr Leu Glu Phe Leu Ala Leu Val Ser Ile Ser Leu 370 375 380 ggc gtg ctg aac ctg ctg ccc gtc ccc gtt ttg gac ggc ggc cac ctc 1200 Gly Val Leu Asn Leu Leu Pro Val Pro Val Leu Asp Gly Gly His Leu 385 390 395 400 gtg ttt tat act gcc gaa tgg ata cgc ggc aaa cct ttg ggc gaa cgc 1248 Val Phe Tyr Thr Ala Glu Trp Ile Arg Gly Lys Pro Leu Gly Glu Arg

405 410 415

gtc caa aac Val Gin Asn atc ggt ttg cgc ttc ggg ctt gcc ctc atg atg ctg atg 1296 Ile 420 Gly Leu Arg Phe Gly 425 Leu Ala Leu Met Met Leu 430 Met atg gcg gtc gcc ttc ttc aac gac gtt acc cgg ctg ctc ggt 1338 Met Ala Val Ala Phe Phe Asn Asp Val Thr Arg Leu Leu Gly 435 440 445

<210> 184 <211> 446 <212> PRT <213> Neisseria meningitidis <400> 184

Leu 1 His Thr Leu Leu 5 Ala Phe Ile Phe Ala 10 Ile Leu Ile Leu Val 15 Ser Leu His Glu Phe 20 Gly His Tyr Ile Val 25 Ala Arg Leu Cys Gly 30 Val Lys Val Val Arg 35 Phe Ser Val Gly Phe 40 Gly Lys Pro Phe Phe 45 Thr Arg Lys Arg Gly Asp Thr Glu Trp Cys Leu Ala Pro Ile Pro Leu Gly Gly Tyr

358

--Μ.

·· ·* ·· · ·· · ···· · · ·· ·· • · β · « · ·

WO 01/85772 PCT/GB01/02003

50 55 60 Val Lys Met Val Asp Thr Arg Glu Gly Glu Val Ser Glu Ala Asp Leu 65 70 75 80 Pro Tyr Ala Phe Asp Lys Gin His Pro Ala Lys Arg Ile Ala Ile Val 85 90 95 Ala Ala Gly Pro Leu Thr Asn Leu Ala Leu Ala Val Leu Leu Tyr Gly 100 105 110 Leu Ser Phe Ser Phe Gly Val Thr Glu Leu Arg Pro Tyr Val Gly Thr 115 120 125 Val Glu Pro Asp Thr Ile Ala Ala Arg Ala Gly Phe Gin Ser Gly Asp 130 135 140 Lys Ile Gin Ser Val Asn Gly Thr Pro Val Ala Asp Trp Gly Ser Ala 145 150 155 160 Gin Thr Glu Ile Val Leu Asn Leu Glu Ala Gly Lys Val Ala Val Gly 165 170 175 Val Gin Thr Ala Ser Gly Ala Gin Thr Val Arg Thr Ile Asp Ala Ala 180 185 190 Gly Thr Pro Glu Ala Gly Lys Ile Ala Lys Asn Gin Gly Tyr Ile Gly 195 200 205 Leu Met Pro Phe Lys Ile Thr Thr Val Ala Gly Gly Val Glu Lys Gly 210 215 220 Ser Pro Ala Glu Lys Ala Gly Leu Lys Pro Gly Asp Arg Leu Thr Ala 225 230 235 240 Ala Asp Gly Lys Pro Ile Ala Ser Trp Gin Glu Trp Ala Asn Leu Thr 245 250 255 Arg Gin Ser Pro Gly Lys Lys Ile Thr Leu Thr Tyr Glu Arg Ala Gly 260 265 270 Gin Thr His Thr Ala Asp Ile Arg Pro Asp Thr Val Glu Gin Pro Asp 275 280 285 His Thr Leu Ile Gly Arg Val Gly Leu Arg Pro Gin Pro Asp Arg Ala 290 295 300 Trp Asp Ala Gin Ile Arg Arg Ser Tyr Arg Pro Ser val Val Arg Ala

359 • · ♦ · · β 9 ··· 99

335

WO 01/85772

305

310

PCT/GBOl/02003

320

315

Phe Gly Met Gly Trp 325 Glu Lys Thr Val Ser 330 His Ser Trp Thr Lys Phe Phe Gly Lys Leu Ile Ser Gly Asn Ala Ser Val Ser 340 345 350 Ser Gly Pro Leu Thr Ile Ala Asp Ile Ala Gly Gin Ser Ala 355 360 365 Gly Leu Gin Ser Tyr Leu Glu Phe Leu Ala Leu Val Ser Ile 370 375 380 Gly Val Leu Asn Leu Leu Pro Val Pro val Leu Asp Gly Gly 385 390 395 Val Phe Tyr Thr Ala Glu Trp Ile Arg Gly Lys Pro Leu Gly 405 410 Val Gin Asn Ile Gly Leu Arg Phe Gly Leu Ala Leu Met Met 420 425 430 Met Ala Val Ala Phe Phe Asn Asp Val Thr Arg Leu Leu Gly 435 440 445

415

400 <210> 185 <211> 1476 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1476) <400> 185

atg aaa tac aaa gac ctg cgc gac ttc atc gcc atg ctc gag cag cag 48 Met Lys Tyr Lys Asp Leu Arg Asp Phe Ile Ala Met Leu Glu Gin Gin 1 5 10 15 ggc aaa ctc aaa cgc atc gcg cac ccc gtt tcc ccg cat ttg gaa atg 96 Gly Lys Leu Lys Arg Ile Ala His Pro Val Ser Pro His Leu Glu Met 20 25 30 acc gaa atc gcc gac cgc gtg ctg cgc gcc gaa ggg ccg gcg ttg ttg 144 Thr Glu Ile Ala Asp Arg Val Leu Arg Ala Glu Gly Pro Ala Leu Leu

360

99 ·· 99 9 999999 ···· 9 9 9« 9 9 9

9 9 9 9 9 · 9 9 • · « 9 · 9999 9

9 9 99 9 9999

WO 01/85772

PCT/GBO1/02003

35 40 45

ttt gaa cac His cca Pro gtt aag ccc Pro 55 gac ggt acg cgc tat gat tat ccc gtg Val 192 Phe Glu 50 Val Lys Asp Gly Thr Arg Tyr 60 Asp Tyr Pro ttg gca aac ctg ttc ggc acg ccc gaa cgt gtg gcg atg ggc atg ggc 240 Leu Ala Asn Leu Phe Gly Thr Pro Glu Arg Val Ala Met Gly Met Gly 65 70 75 80 gcg gac agc gtg tcc aag ctg cgc gaa atc ggg cag acg ctg gcg tat 288 Ala Asp Ser Val Ser Lys Leu Arg Glu Ile· Gly Gin Thr Leu Ala Tyr 85 90 95 ttg aaa gaa ccc gaa ccg ccc aaa ggc att aaa gac gcg ttt tcc aaa 336 Leu Lys Glu Pro Glu Pro Pro Lys Gly Ile Lys Asp Ala Phe Ser Lys 100 105 110 ctg ccg ctc ttg aaa gac att tgg agc atg gcg ccg aac gtg gtg aaa 384 Leu Pro Leu Leu Lys Asp Ile Trp Ser Met Ala Pro Asn Val Val Lys 115 120 125 aat gcg ccg tgt cag gaa atc gta tgg gaa ggc gaa gac gtt gat ttg 432 Asn Ala Pro Cys Gin Glu Ile Val Trp Glu Gly Glu Asp Val Asp Leu 130 135 140 tat caa ctt ccg att cag cat tgc tgg ccg gaa gac gtt gcg ccg ctg 480 Tyr Gin Leu Pro Ile Gin His Cys Trp Pro Glu Asp Val Ala Pro Leu 145 150 155 160 gta acg tgg ggc ttg acc gtc acg cgc ggg ccg cac aaa aaa cgc caa 528 Val Thr Trp Gly Leu Thr Val Thr Arg Gly Pro His Lys Lys Arg Gin 165 170 175 aat ctc ggc att tac cgc caa caa tta atc ggc ata aac aag ctg att 576 Asn Leu Gly Ile Tyr Arg Gin Gin Leu Ile Gly Ile Asn Lys Leu Ile 180 185 190 atg cgc tgg ctg tcg cat cgc ggc ggc gcg ctg gat tat cag gaa ttc 624 Met Arg Trp Leu Ser His Arg Gly Gly Ala Leu Asp Tyr Gin Glu Phe 195 200 205 cgc aaa ctc aat ccc gat acg ccg tat ccc gtc gcc gtc gtg ctc ggc 672 Arg Lys Leu Asn Pro Asp Thr Pro Tyr Pro Val Ala Val Val Leu Gly 210 215 220 tgc gac cct gcc acc att ttg ggc gcg gta acg ccc gtt ccc gat acc 720 Cys Asp Pro Ala Thr Ile Leu Gly Ala val Thr Pro Val Pro Asp Thr

361 ·· « · w ·

WO 01/85772

PCT/GB01/02003

225

230 235 240

ttg agc gaa tac cag ttt gcc gga ctg ctg cgc ggt tcg cgg acg gaa 768 Leu Ser Glu Tyr Gin Phe 245 Ala Gly Leu Leu 250 Arg Gly Ser Arg Thr Glu 255 ctg gtg aaa tgt atc ggc aac gat ttg caa gtg cct gcc cgc gca gaa 816 Leu Val Lys Cys Ile Gly Asn Asp Leu Gin Val Pro Ala Arg Ala Glu 260 265 270 atc gtg ttg gaa ggc gtc atc cat cca aac gaa acc gcg ttg gaa ggt 864 Ile Val Leu Glu Gly Val Ile His Pro Asn Glu Thr Ala Leu Glu Gly 275 280 285 cca tac ggc gac cac acg ggc tat tac aac gag cag gac cat ttc ccc 912 Pro Tyr Gly Asp His Thr Gly Tyr Tyr Asn Glu Gin Asp His Phe Pro 290 295 300 gtg ttt acg gtc gaa cgc atc acc atg cgc gaa aac ccg att tac cac 960 Val Phe Thr Val Glu Arg Ile Thr Met Arg Glu Asn Pro Ile Tyr His 305 310 315 320 tet acc tac aca ggc aaa ccg ccc gat gaa ccc gcc gtt ttg ggc gtg 1008 Ser Thr Tyr Thr Gly Lys Pro Pro Asp Glu Pro Ala Val Leu Gly Val 325 330 335 gcg ttg aac gaa gtg ttc gta ccg ctt ttg caa aag cag ttc ccc gaa 1056 Ala Leu Asn Glu Val Phe Val Pro Leu Leu Gin Lys Gin Phe Pro Glu 340 345 350 atc acc gat ttt tac ctg ccg ccc gaa ggc tgc tcc tac cgc atg gcg 1104 Ile Thr Asp Phe Tyr Leu Pro Pro Glu Gly Cys Ser Tyr Arg Met Ala 355 360 365 gtg gtg agc atg aaa aaa cag tac gcc gga cac gcc aag cgc gtg atg 1152 Val Val Ser Met Lys Lys Gin Tyr Ala Gly His Ala Lys Arg Val Met 370 375 380 atg ggc tgc tgg tcg ttc ctg cgc cag ttt atg tac acc aaa ttc atc 1200 Met Gly Cys Trp Ser Phe, Leu Arg Gin Phe Met Tyr Thr Lys Phe Ile 385 390 395 400 att gtg gtg gat gac gat gtg gat gtg cgc gac tgg aaa gaa gtc atc 1248 Ile Val Val. Asp Asp Asp Val Asp Val Arg Asp Trp Lys Glu Val Ile 405 410 415 tgg gcg gta acc acg cgc atg gac ccc gtg cgc gat acc gtt ttg atg 1296 Trp Ala Val Thr Thr Arg Met Asp Pro Val Arg Asp Thr Val Leu Met

362 • · «fcfcfc • fc fc· fc « t · ·· • · · e fc fc······· • fc · fc * • · · · ···· · • · · · · • •e fcfc ·<

WO 01/85772 PCT/GB01/02003

420 425 430 gaa aac acg ccc atc gac tac ctc gac ttc gcc agc ccc gtc agc gga 1344 Glu Asn Thr Pro Ile Asp Tyr Leu Asp Phe Ala Ser Pro Val Ser Gly 435 440 445 ctt ggc ggc aaa atg ggt ttg gat gcg acc aac aag tgg ccg ggc gaa 1392 Leu Gly Gly Lys Met Gly Leu Asp Ala Thr Asn Lys Trp Pro Gly Glu 450 455 460 acc gac cgc gaa tgg gga cgg gtg att aaa aaa gac cct gcg gtt acg 1440 Thr Asp Arg Glu Trp Gly Arg Val Ile Lys Lys Asp Pro Ala Val Thr 465 470 475 480 gct aag att gat gag att tgg gag gaa ttg ggg ttg 1476 Ala Lys Ile Asp Glu Ile Trp Glu Glu Leu Gly Leu 485 490

<210> 186 <211> 492 <212> PRT <213> Neisseria meningitidis <400> 186

Met 1 Lys Tyr Lys Asp 5 Leu Arg Asp Phe Ile 10 Ala Met Leu Glu Gin 15 Gin Gly Lys Leu Lys 20 Arg Ile Ala His Pro 25 Val Ser Pro His Leu 30 Glu Met Thr Glu Ile 35 Ala Asp Arg Val Leu 40 Arg Ala Glu Gly Pro 45 Ala Leu Leu Phe Glu 50 His Pro Val Lys Pro 55 Asp Gly Thr Arg Tyr Asp 60 Tyr Pro Val Leu 65 Ala Asn Leu Phe Gly 70 Thr Pro Glu Arg Val 75 Ala Met Gly Met Gly 80 Ala Asp Ser Val Ser 85 Lys Leu Arg Glu Ile 90 Gly Gin Thr Leu Ala 95 Tyr Leu Lys Glu Pro 100 Glu Pro Pro Lys Gly 105 Ile Lys Asp Ala Phe 110 Ser Lys Leu Pro Leu Leu Lys Asp Ile Trp Ser Met Ala Pro Asn Val val Lys

115 120 125

363 • · · · · · · ·· ···· ♦ · · · ·· · o · β β β β β · · ········ ·· ··· ·· ··

W0 01/85772 PCT/GBO1/O2OO3

Asn Ala 130 Pro Cys Gin Glu Ile 135 Val Trp Glu Gly Glu 140 Asp Val Asp Leu Tyr 145 Gin Leu Pro Xle Gin 150 His Cys Trp Pro Glu 155 Asp Val Ala Pro Leu 160 Val Thr Trp Gly Leu 165 Thr Val Thr Arg Gly 170 Pro His Lys Lys Arg 175 Gin Asn Leu Gly Ile 180 Tyr Arg Gin Gin Leu 185 Ile Gly Ile Asn Lys 190 Leu Ile Met Arg Trp 195 Leu Ser His Arg Gly 200 Gly Ala Leu Asp Tyr 205 Gin Glu Phe Arg Lys 210 Leu Asn Pro Asp Thr 215 Pro Tyr Pro Val Ala 220 Val Val Leu Gly Cys 225 Asp Pro Ala Thr Ile 230 Leu Gly Ala Val Thr 235 Pro Val Pro Asp Thr 240 Leu Ser Glu Tyr Gin 245 Phe Ala Gly Leu Leu 250 Arg Gly Ser Arg Thr 255 Glu Leu Val Lys Cys 260 Ile Gly Asn Asp Leu 265 Gin Val Pro Ala Arg 270 Ala Glu Ile Val Leu 275 Glu Gly Val Ile His 280 Pro Asn Glu Thr Ala 285 Leu GlU Gly Pro Tyr 290 Gly Asp His Thr Gly 295 Tyr Tyr Asn Glu Gin 300 Asp His Phe Pro Val 305 Phe Thr Val Glu Arg 310 Ile Thr Met Arg Glu 315 Asn Pro Ile Tyr His 320 Ser Thr Tyr Thr Gly 325 Lys Pro Pro Asp Glu 330 Pro Ala Val Leu Gly 335 Val Ala Leu Asn Glu 340 Val Phe Val Pro Leu 345 Leu Gin Lys Gin Phe 350 Pro Glu Ile Thr Asp 355 Phe Tyr Leu Pro Pro 360 Glu Gly Cys Ser Tyr Arg 365 Met Ala Val Val Ser Met Lys Lys Gin Tyr Ala Gly His Ala Lys Arg Val Met

370 375 380

364

WO 01/85772

PCT/GBO1/02003

Met Gly Cys Trp Ser Phe Leu Arg Gin Phe Met Tyr Thr Lys Phe Ile 385 390 395 400 Ile Val Val Asp Asp Asp Val Asp Val Arg Asp Trp Lys Glu Val Ile 405 410 415 Trp Ala Val Thr Thr Arg Met Asp Pro Val Arg Asp Thr Val Leu Met 420 425 430 Glu Asn Thr Pro Ile Asp Tyr Leu Asp Phe Ala Ser Pro Val Ser Gly 435 440 445 Leu Gly Gly Lys Met Gly Leu Asp Ala Thr Asn Lys Trp Pro Gly Glu 450 455 460 Thr Asp Arg Glu Trp Gly Arg Val Ile Lys Lys Asp Pro Ala Val Thr 465 470 ⁴⁷⁵ 480 Ala Lys Ile Asp Glu Ile Trp Glu Glu Leu Gly Leu 485 490

<210> 187 <211> 1191 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1191) <400> 187

atg ttc ttc aag cac atc gaa gcc gcc ccc gcc gat ccg att ctc ggt Met Phe Phe Lys His Ile Glu Ala Ala Pro Ala Asp Pro Ile Leu Gly 1 5 10 15 ttg ggc gaa gca ttc aaa gcc gaa acc cgc ccc gaa aaa gtc aac ctc Leu Gly Glu Ala Phe Lys Ala Glu Thr Arg Pro Glu Lys Val Asn Leu 20 25 30 ggc atc ggc gtg tac aaa gac gca tcc ggc gcg aca cct att gtc aaa Gly Ile Gly Val Tyr Lys Asp Ala Ser Gly Ala Thr Pro Ile Val Lys 35 40 45 gcc gtc aaa gaa gcc gaa aaa cgc ctg ttg gaa age gaa aca act aaa Ala Val Lys Glu Ala Glu Lys Arg Leu Leu Glu Ser Glu Thr Thr Lys

144

192

365

WO 01/85772 55 60 PCT/GB01/02003 50 aac tac ctc acc atc gac ggc gtt gcc gac tac aac gag caa acc caa 240 Asn. Tyr Leu Thr Ile Asp Gly Val Ala Asp Tyr Asn Glu Gin Thr Gin 65 70 75 80 att ctg ctg ttc ggc aaa gac cac gaa atc atc gcc age cgt cgc gcc 288 Ile Leu Leu Phe Gly Lys Asp His Glu Ile Ile Ala Ser Arg Arg Ala 85 90 95 aaa aca gcg caa age ctt ggc ggt aca ggc gca ttg cgt att gcg gcc 336 Lys Thr Ala Gin Ser Leu Gly Gly Thr Gly Ala Leu Arg Ile Ala Ala 100 105 110 gag ttt gcc aaa cgt cag ttg aac gcg caa acc atc tgg att tcc aat 384 Glu Phe Ala Lys Arg Gin Leu Asn Ala Gin Thr Ile Trp Ile Ser Asn 115 120 125 ccg act tgg ccc aac cac aac gcc atc gcc aaa gcg gtc ggt atc caa 432 Pro Thr Trp Pro Asn His Asn Ala Ile Ala Lys Ala Val Gly Ile Gin 130 135 140 gac caa cct tat cgc tac tat gat gcc gcc aaa cac ggt ttg gat tgg 480 Asp Gin Pro Tyr Arg Tyr Tyr Asp Ala Ala Lys His Gly Leu Asp Trp 145 150 155 160 gac ggt atg att gaa gac ttg age caa gcg caa aaa ggc gac atc gtc 528 Asp Gly Met Ile Glu Asp Leu Ser Gin Ala Gin Lys Gly Asp Ile Val 165 170 175 ctg ctg cac ggc tgc tgc cac aac cct acc ggt atc gac cct acg ccc 576 Leu Leu His Gly Cys Cys His Asn Pro Thr Gly Ile Asp Pro Thr Pro 180 185 190 gaa caa tgg gaa act ctg gca aaa ctt tet gcc gaa aaa ggc tgg tta 624 Glu Gin Trp Glu Thr Leu Ala Lys Leu Ser Ala Glu Lys Gly Trp Leu 195 200 205 ccg ctg ttt gac ttt gcc tac caa ggc ttc ggc aat ggt ttg gaa gaa 672 Pro Leu Phe Asp Phe Ala. ,Tyr Gin Gly Phe Gly Asn Gly Leu Glu Glu 210 215 220 gat gcc tac ggc ctg cgc gtg ttc ttg aaa cac aat aca gaa ttg ctg 720 Asp Ala Tyr Gly Leu Arg Val Phe Leu Lys His Asn Thr Glu Leu Leu 225 230 235 240 att gcc age tet tat tcc aaa aac ttc ggt atg tac aac gag cgc gtc 768 Ile Ala Ser Ser Tyr Ser Lys Asn Phe Gly Met Tyr Asn Glu Arg Val

366

WO 01/85772 PCT/GB01/02003

245 250 255 ggc gcg ttc act ttg gtg gcc gaa gat gaa gca acc gcc gcc cgc gcc 816 Gly Ala Phe Thr Leu Val Ala Glu Asp Glu Ala Thr Ala Ala Arg Ala 260 265 270 cac age caa gtc aaa acc att atc cgt acc ttg tat tcc aac cct gct 864 His Ser Gin Val Lys Thr Ile Ile Arg Thr Leu Tyr Ser Asn Pro Ala 275 280 285 tea cac ggc gcg aac act att gcg ctg gtg ttg aaa aat gat gat ttg 912 Ser His Gly Ala Asn Thr Ile Ala Leu Val Leu Lys Asn Asp Asp Leu 290 295 300 aaa gca caa tgg att gcc gaa ctc gat gaa atg cgc ggc cgt atc aaa 960 Lys Ala Gin Trp Ile Ala Glu Leu Asp Glu Met Arg Gly Arg Ile Lys 305 310 315 320 gcc atg cgc caa aaa ttt gtc gag ttg ctc aaa gcc aaa ggt gca act 1008 Ala Met Arg Gin Lys Phe Val Glu Leu Leu Lys Ala Lys Gly Ala Thr 325 330 335 caa gac ttt gat ttc att atc gaa caa aac ggc atg ttc tcc ttc age 1056 Gin Asp Phe Asp Phe Ile Ile Glu Gin Asn Glý Met Phe Ser Phe Ser 340 345 350 ggc ttg act ccc gaa caa gtc gac cgt tta aaa aac gag ttt gcc att 1104 Gly Leu Thr Pro Glu Gin Val Asp Arg Leu Lys Asn Glu Phe Ala Ile 355 360 365 tat gcc gtc cgc tcc ggc cgc atc aac gtc gcc ggc att acc gac gac 1152 Tyr Ala Val Arg Ser Gly Arg Ile Asn Val Ala Gly Ile Thr Asp Asp 370 375 380 aac atc gat tat ctg tgc gaa agt atc gta aaa gta ctg 1191 Asn Ile Asp Tyr Leu Cys Glu Ser Ile Val Lys Val Leu 385 390 395

<210> 188 <211> 397 <212> PRT <213> Neisseria meningitidis <400> 188

Met Phe Phe Lys His Ile Glu Ala Ala Pro Ala Asp Pro Ile Leu Gly 15 10 15

367

• · • • e β « • · • · · ,-e-. - - _e-— · • i β íc«e • · • 9 -«·· . • · Sí • · · · < • 9 · · _ __._e. .. —e-----e- - • · · · e e β 9 9 < WO 01/85772 PCT/GB01/02003 Leu Gly Glu Ala Phe Lys 20 Ala Glu Thr Arg Pro Glu 25 Lys Val 30 Asn Leu Gly Ile Gly Val Tyr Lys 35 Asp Ala Ser Gly Ala Thr 40 Pro Ile 45 Val Lys Ala val Lys Glu Ala Glu 50 Lys Arg Leu Leu Glu Ser 55 60 Glu Thr Thr Lys Asn Tyr Leu Thr Ile Asp 65 70 Gly Val Ala Asp Tyr Asn 75 Glu Gin Thr Gin 80 Ile Leu Leu Phe Gly Lys 85 Asp His Glu Ile Ile Ala 90 Ser Arg Arg 95 Ala Lys Thr Ala Gin Ser Leu 100 Gly Gly Thr Gly Ala Leu 105 Arg Ile 110 Ala Ala Glu Phe Ala Lys Arg Gin 115 Leu Asn Ala Gin Thr Ile 120 Trp Ile 125 Ser Asn Pro Thr Trp Pro Asn His 130 · Asn Ala Ile Ala Lys Ala 135 140 Val Gly Ile Gin Asp Gin Pro Tyr Arg Tyr 145 150 Tyr Asp Ala Ala Lys His 155 Gly Leu Asp Trp 160 Asp Gly Met Ile Glu Asp 165 Leu Seř Gin Ala Gin Lys 170 Gly Asp Ile 175 Val Leu Leu His Gly Cys Cys 180 His Asn Pro Thr Gly Ile 185 Asp Pro 190 Thr Pro Glu Gin Trp Glu Thr Leu 195 Ala Lys Leu Ser Ala Glu 200 Lys Gly 205 Trp Leu Pro Leu Phe Asp Phe Ala 210 Tyr Gin Gly Phe Gly Asn 215 220 Gly Leu Glu Glu Asp Ala Tyr Gly Leu Arg.-Val Phe Leu Lys His Asn 225 230 235 Thr Glu Leu Leu 240 Ile Ala Ser Ser Tyr Ser 245 Lys Asn Phe Gly Met Tyr 250 Asn Glu Arg 255 Val Gly Ala Phe Thr Leu Val 260 Ala GlU Asp Glu Ala Thr 265 Ala Ala 270 Arg Ala

368

WO 01/85772

PCT/GBO1/02003

His Ser Gin Val Lys Thr Xle Ile Arg Thr Leu Tyr; Ser Asn Pro Ala 275 280 285 Ser His Gly Ala Asn Thr Ile Ala Leu Val Leu Lys Asn Asp Asp Leu 290 295 300 Lys Ala Gin Trp Ile Ala Glu Leu Asp Glu Met Arg Gly Arg Ile Lys 305 310 315 320 Ala Met Arg Gin Lys Phe Val Glu Leu Leu Lys Ala Lys Gly Ala Thr 325 330 335 Gin Asp Phe Asp Phe Ile Ile Glu Gin Asn Gly Met Phe Ser Phe Ser 340 345 350 Gly Leu Thr Pro Glu Gin Val Asp Arg Leu Lys Asn Glu Phe Ala Ile 355 360 365 Tyr Ala Val Arg Ser Gly Arg Ile Asn Val Ala Gly Ile Thr Asp Asp 370 375 380 Asn Ile Asp Tyr Leu Cys Glu Ser Ile Val Lys Val Leu 385 390 395

<210> 189 <211> 462 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1) .. (462) <400> 189 ttg ctt tgc ccg gaa aaa atg tcg ggg atg gcg gga cag tat ccg tac 48

Leu Leu Cys Pro Glu Lys Met Ser Gly Met Ala Gly Gin Tyr Pro Tyr

1 5 10 15 *

ggc gtc cgg tcg ggt ttg cgg agg aac ggc ttg aaa ctt tgg gat att 96

Gly Val Arg Ser Gly Leu Arg Arg Asn Gly Leu Lys Leu Trp Asp ile

20 25 30 cat ttt aga atg acc cgt ttt atc gtc gca aga tgc ggt tta ttg ttt 144

His Phe Arg Met Thr Arg Phe Ile Val Ala Arg Cys Gly Leu Leu Phe

35 40 45

369

WO 01/85772

PCT/GBO1/02003

gca acc ctt aaa gga aaa acc atg Ala Thr 50 Leu Lys Gly Lys Thr 55 Met atg ctg ttc tcc tgc gcc ttc tcc Met 65 Leu Phe Ser Cys Ala 70 Phe Ser gct tcg cag cag gag ctg gag gcg Ala Ser Gin Gin Glu 85 Leu Glu Ala gcg aag gcc att gcg gaa tac cgt Ala Lys Ala Ile 100 Ala Glu Tyr Arg gta gac gat ttg acc aag gta aag Val Asp Asp 115 Leu Thr Lys Val Lys 120 aag ctg aag gat cag gct tet gtc Lys Leu 130 Lys Asp Gin Ala Ser 135 Val gca aaa ccg gct ctg ccc gca gct Ala 145 Lys Pro Ala Leu Pro 150 Ala Ala

aag aaa atg ttc Lys Lys Met Phe gtg ctg ttc tgt Val Leu Phe Cys 192 60 ctt gcg gcg gta aac atc aat gcg 240 Leu Ala Ala Val Asn Ile Asn Ala 75 80 ctg ccg ggc ata ggc cct gcg aag 288 Leu Pro Gly Ile Gly Pro Ala Lys 90 95 gcg caa aac ggt gcg ttc aag tet 336 Ala Gin Asn Gly Ala Phe Lys Ser 105 110 ggc atc ggc cct gcg gtg ctg gcg 384 Gly Ile Gly Pro Ala Val Leu Ala 125 ggt gcg ccc gca cca aaa ggc ccg 432 Gly Ala Pro Ala Pro Lys Gly Pro

140 aaa aaa . 462

Lys Lys <210> 190 <211> 154 <212> PRT

<213> Neisseria meningitidis <400> 190 Leu Leu Cys Pro Glu Lys Met Ser Gly Met Ala Gly Gin Tyr Pro Tyr 15 10 15 Gly Val Arg Ser Gly Leu Arg Arg Asn Gly Leu Lys Leu Trp Asp Ile 20 25 30 His Phe Arg Met Thr Arg Phe Ile Val Ala Arg Cys Gly Leu Leu Phe 35 40 45 Ala Thr Leu Lys Gly Lys Thr Met Lys Lys Met Phe Val Leu Phe Cys 50 55 60 Met Leu Phe Ser Cys Ala Phe Ser Leu Ala Ala Val Asn Ile Asn Ala

370 • 0 • 0 ··· ·

• 0 0· 00 0 «00000 0000 0 0 0 0 00 0 • 0 0 0 0 0 0 0 0 0 000 0000 0 0 00 0000 «0 00« 0 0 *0 WO 01/85772 PCT/GB01/02003 65 70 75 80

Ala Ser Gin Gin Glu 85 Leu Glu Ala Leu Pro 90 Gly Ile Gly Pro Ala 95 Lys Ala Lys Ala Xle 100 Ala Glu Tyr Arg Ala 105 Gin Asn Gly Ala Phe 110 Lys Ser Val Asp Asp 115 Leu Thr Lys Val Lys 120 Gly Xle Gly Pro Ala 125 Val Leu Ala Lys Leu 130 Lys Asp Gin Ala Ser 135 Val Gly Ala Pro Ala 140 Pro Lys Gly Pro Ala Lys Pro Ala Leu Pro Ala Ala Lys Lys

145 150 <210> 191 <211> 684 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(684) <400> 191

atg Met 1 ttt gct ttt tta Leu 5 gaa gcc ttt ttt gtc gaa tac ggt tat gcg gct 48 Phe Ala Phe Glu Ala Phe Phe Val Glu Tyr Gly Tyr Ala Ala 10 15 gtt ttt ttt gta ttg gtc atc tgc ggt ttc ggc gtg ccg att ccc gag 96 Val Phe Phe Val Leu Val Ile Cys Gly Phe Gly Val Pro Ile Pro Glu 20 25 30 gat ttg acc ttg gta aca ggc ggc gtg att tcg ggt atg ggt tat acc 144 Asp Leu Thr Leu Val Thr Gly Gly Val Ile Ser Gly Met Gly Tyr Thr 35 40 45 aat ccg cat att atg ttt gca gtc ggt atg ctc ggc gta ttg gtc ggg 192 Asn Pro His Ile Met Phe Ala Val Gly Met Leu Gly Val Leu Val Gly 50 55 60 gac ggc atc atg ttc gcc gcc gga ega att tgg ggg cag aaa atc cta 240 Asp Gly Ile Met Phe Ala Ala Gly Arg Ile Trp Gly Gin Lys Ile Leu

65 70 75 80

371 φφφφ φφφφ

WO 01/85772 PCT/GB01/02003

agg ttc aaa cct Pro att gcg cgc atc atg acg ccg aaa cgt tat gag cag 288 Arg Phe Lys Ile Ala 85 Arg Ile Met Thr 90 Pro Lys Arg Tyr Glu Gin 95 gtt cag gaa aaa ttc gac aaa tac ggt aac tgg gtc tta ttt gtc gcc 336 Val Gin Glu Lys Phe Asp Lys Tyr Gly Asn Trp Val Leu Phe Val Ala 100 105 110 cgt ttc ctg ccc ggt ttg aga acg gcc gta ttt gtt aca gcc ggt atc 384 Arg Phe Leu Pro Gly Leu Arg Thr Ala Val Phe Val Thr Ala Gly Ile 115 120 125 agc cgc aag gtt tea tac ttg cgt ttt atc att atg gat gga ctg gcc 432 Ser Arg Lys Val Ser Tyr Leu Arg Phe Ile Ile Met Asp Gly Leu Ala 130 135 140 gca ctg att tcc gtc cct att tgg att tat ctg ggc gaa tac ggt gcg 480 Ala Leu Ile Ser Val Pro Ile Trp Ile Tyr Leu Gly Glu Tyr Gly Ala 145 150 155 160 cac aac atc gat tgg ctg atg gcg aaa atg cac agc ctg caa tcg ggt 528 His Asn Ile Asp Trp Leu Met Ala Lys Met His Ser Leu Gin Ser Gly 165 170 175 att ttt gtt atc ttg ggt ata ggt gcg acc gtt gtc gct tgg att tgg 576 Ile Phe Val Ile Leu Gly Ile Gly Ala Thr Val Val Ala Trp Ile Trp 180 185 190 tgg aaa aaa cgc caa cgt atc cag ttt tac cgc agc aaa ttg aaa gaa 624 Trp Lys Lys Arg Gin Arg Ile Gin Phe Tyr Arg Ser Lys Leu Lys Glu 195 20Ó 205 aag cgg gcg caa cgc aaa gcc gcc aag gca gcc aaa aaa gcc gcg caa 672 Lys Arg Ala Gin Arg Lys Ala Ala Lys Ala Ala Lys Lys Ala Ala Gin 210 215 220

agc aaa caa taa 684

Ser Lys Gin

225 , <210> 192 <211> 227 <212> PRT <213> Neisseria meningitidis <4OO> 192

372 titi9Stt

WO 01/85772 PCT/GB01/02003

Met 1 Phe Ala Phe Leu 5 Glu Ala Phe Phe Val 10 Glu Tyr Gly Tyr Ala 15 Ala val Phe Phe Val 20 Leu Val Ile Cys Gly 25 Phe Gly Val Pro Ile 30 Pro Glu Asp Leu Thr 35 Leu Val Thr Gly Gly 40 Val Ile Ser Gly Met 45 Gly Tyr· Thr Asn Pro 50 His Ile Met Phe Ala 55 Val Gly Met Leu Gly 60 Val Leu Val Gly Asp 65 Gly Ile Met Phe Ala 70 Ala Gly Arg Ile Trp 75 Gly Gin Lys Ile Leu 80 Arg Phe Lys Pro Ile 85 Ala Arg ile Met Thr 90 Pro Lys Arg Tyr Glu 95 Gin Val Gin Glu Lys 100 Phe Asp Lys Tyr Gly 105 Asn Trp Val Leu Phe 110 Val Ala Arg Phe Leu 115 Pro Gly Leu Arg Thr 120 Ala Val Phe Val Thr 125 Ala Gly Ile Ser Arg 130 Lys Val Ser Tyr Leu 135 Arg Phe Ile Ile Met 140 Asp Gly Leu Ala Ala 145 Leu Ile Ser Val Pro 150 Ile Trp Ile Tyr Leu 155 Gly Glu Tyr Gly Ala 160 His Asn Ile Asp Trp 165 Leu Met Ala Lys Met 170 His Ser Leu Gin Ser 175 Gly Ile Phe Val Ile 180 Leu Gly Ile Gly Ala 185 Thr Val Val Ala Trp 190 Ile Trp Trp Lys Lys 195 Arg Gin Arg Ile Gin 200 Phe Tyr Arg Ser Lys 205 Leu Lys Glu Lys Arg 210 Ala Gin Arg Lys_AAla 215 Ala Lys Ala Ala Lys 220 Lys Ala Ala Gin

Ser Lys Gin 225 <210> 193

373 • fc fc* ·* · fc fcfc · » fc · · * fc · « » fc fcfcfcfc · ·· e © fc ···· ·«·· ·· ···

WO 01/85772 PCT/GB01/02003 <211> 1089 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(1089, <400> 193

atg aat gac tac acg cag cag ctt caa ggt aaa aaa gac tac ctt aaa 48 Met 1 Asn Asp Tyr Thr 5 Gin Gin Leu Gin Gly Lys Lys Asp Tyr Leu Lys 10 15 acc ctt ttt gca ggt ttg gat gtt cct gag tgg gaa gtg tac gaa tet 96 Thr Leu Phe Ala Gly Leu Asp Val Pro GlU Trp Glu Val Tyr Glu Ser 20 25 30 ccg gac aaa cat tac cgt atg cgt gcc gag ttc cgt att tgg cac gaa 144 Pro Asp Lys His Tyr Arg Met Arg Ala Glu Phe Arg Ue Trp His Glu 35 40 45 ggc ggg gaa atg ttt tat gcc atg ttt gaa aaa ggg cag aaa gcc. agc 192 Gly Gly Glu Met Phe Tyr Ala Met Phe Glu Lys Gly Gin Lys Ala Ser 50 55 60 ggc gca agc atg ata cgc tgc gac cgt ttt gaa gca gct tcc gag gct 240 Gly Ala Ser Met Ue Arg Cys Asp Arg Phe Glu Ala Ala Ser Glu Ala 65 70 75 80 gtc aac cgc ctc atg ccc gag ctg atc gcc gcc gcc gcg caa tcc ccc 288 Val Asn Arg Leu Met Pro Glu Leu Ile Ala Ala Ala Ala Gin Ser Pro 85 90 95 gaa ctc aaa aaa cgc tgg tat gcc gtc gaa ttt ctg tcc acg ctc agc 336 Glu Leu Lys Lys Arg Trp Tyr Ala Val Glu Phe Leu Ser Thr Leu Ser 100 105 110 ggg gaa atg ctg gtt acc atg att tac cac aaa agg ctt gat gct gag 384 Gly Glu Met Leu Val Thr Met Ue Tyr His Lys Arg Leu Asp Ala Glu 115 120 125 tgg atg cag gcg gcg caa gcg tta cag caa cag ttg gat att tcc gtt 432 Trp Met Gin Ala Ala Gin Ala Leu Gin Gin Gin Leu Asp Ue Ser Val 130 135 140 att ggg cgg agc agg gga cag aaa ata gtc tta aaa cag gac tat gta 480 Ile Gly Arg Ser Arg Gly Gin Lys Ile Val Leu Lys Gin Asp Tyr Val 145 150 155 160

374 • · · ·

WO 01/85772 e e ec s « s«

PCT/GB01/02003

acg gaa act ttg aag gtc ggc aac cgg gat ttc cgt tat cgg caa att Ile 528 Thr Glu Thr Leu Lys Val 165 Gly Asn Arg Asp 170 Phe Arg Tyr Arg Gin 175 gaa ggc agt ttt acc caa ccg aat gcc gcc gtg tgt cag aaa atg ctt 576 Glu Gly Ser Phe Thr Gin Pro Asn Ala Ala Val Cys Gin Lys Met Leu ISO 185 190 gaa tgg gcg tgc cgc acg gcg gaa ggc ttg ggc age gac ctg ctc gaa 624 Glu Trp Ala Cys Arg Thr Ala Glu Gly Leu Gly Ser Asp Leu Leu Glu 195 200 205 ctg tat tgc gga aac ggc aat ttc acc ctg ccg ctg tcc ega tat ttc 672 Leu Tyr Cys Gly Asn Gly Asn Phe Thr Leu Pro Leu Ser Arg Tyr Phe 210 215 220 ega cag gtt ttg gca acc gaa atc tcc aaa acc tcc gtc age gcg gcg 720 Arg Gin Val Leu Ala Thr Glu Ile Ser Lys Thr Ser Val Ser Ala Ala 225 230 235 240 caa tgg aat atc gaa gcc aac cgc atc ggc aac atc aaa atc gcc cgt 768. Gin Trp Asn Ile Glu Ala Asn Arg Ile Gly Asn Ile Lys Ile Ala Arg 245 250 255 ctg tet gct gaa gaa ttt acc gaa gcc tat acc gga aaa cgt gag ttt 816 Leu Ser Ala Glu Glu Phe Thr Glu Ala Tyr Thr Gly Lys Arg Glu Phe 260 265 270 aaa cgc ctt aag gat ggc ggt att gct ttg acg gat tac gca ttt tcc 864 Lys Arg Leu Lys Asp Gly Gly Ile Ala Leu Thr Asp Tyr Ala Phe Ser 275 280 285 act att ttc gtc gat ccg ccg cgt gcc ggt atc gat gag gaa act ttg 912 Thr Ile Phe Val Asp Pro Pro Arg Ala Gly Ile Asp Glu Glu Thr Leu 290 295 300 aag ctg gtg tcg cag ttt gac aac atc atc tac att tcc tgc aat ccc 960 Lys Leu Val Ser Gin Phe Asp Asn Ile Ile Tyr Ile Ser Cys Asn Pro 305 310 315 320 gaa acg ttg cgc gcc aat ttg gac acc ttg gcg gaa acc cat gcg gtc 1008 Glu Thr Leu Arg Ala Asn Leu Asp Thr Leu Ala Glu Thr His Ala Val 325 330 335 gaa cgt gcc gcc ctg ttc gac caa ttc ccg ttt acg cac cat atc gaa 1056 Glu Arg Ala Ala Leu Phe Asp Gin Phe Pro Phe Thr His His Ile Glu

340 345 350

375 • · · ·

WO 01/85772 PCT/GBO1/02003 agt ggt gta ttg ttg aaa aag aaa atc ctt tga 1089

Ser Gly Val Leu Leu Lys Lys Lys Ile Leu

355 360 <210> 194 <2U> 362 <212> PRT <213> Neisseria meningitidis <400> 194

Met 1 Asn Asp Tyr Thr 5 Gin Gin Leu Gin Gly 10 Lys Lys Asp Tyr Leu 15 Lys Thr Leu Phe Ala 20 Gly Leu Asp Val Pro 25 Glu Trp Glu Val Tyr 30 Glu Ser Pro Asp Lys 35 His Tyr Arg Met Arg 40 Ala Glu Phe Arg Ile 45 Trp His Glu Gly Gly 50 Glu Met Phe Tyr Ala 55 Met Phe Glu Lys Gly 60 Gin Lys Ala Ser Gly 65 Ala Ser Met Ile Arg 70 Cys Asp Arg Phe Glu 75 Ala Ala Ser Glu Ala 80 val Asn Arg Leu Met 85 Pro Glu Leu Ile Ala 90 Ala Ala Ala Gin Ser 95 Pro Glu Leu Lys Lys 100 Arg Trp Tyr Ala Val 105 Glu Phe Leu Ser Thr 110 Leu Ser Gly Glu Met 115 Leu Val Thr Met Ile 120 Tyr His Lys Arg Leu 125 Asp Ala Glu Trp Met 130 Gin Ala Ala Gin Ala 135 Leu Gin Gin Gin Leu 140 Asp Ile Ser Val Ile 145 Gly Arg Ser Arg Gly, .Gin 150 Lys Ile Val Leu 155 Lys Gin Asp Tyr Val 160 Thr Glu Thr Leu Lys 165 Val Gly Asn Arg Asp 170 Phe Arg Tyr Arg Gin 175 Ile Glu Gly Ser Phe Thr Gin Pro Asn Ala Ala Val Cys Gin Lys Met Leu

180 185 Í90

376

0· ·« ·* · ·· ···· »··· ···· · · · • ο β β e © e e β ··· ···· ·· ··· ·· ··

WO 01/85772 PCT/GB01/02003 Glu Trp Ala Cys Arg Thr Ala Glu Gly Leu Gly Ser Asp Leu Leu Glu 195 200 205 Leu Tyr Cys Gly Asn Gly Asn Phe Thr Leu Pro Leu Ser Arg Tyr Phe 210 215 220 Arg Gin Val Leu Ala Thr Glu Ile Ser Lys Thr Ser Val Ser Ala Ala 225 230 235 240 Gin Trp Asn Ile Glu Ala Asn Arg Ile Gly Asn Ile Lys Ile Ala Arg 245 250 255 Leu Ser Ala Glu Glu Phe Thr Glu Ala Tyr Thr Gly Lys Arg Glu Phe 260 265 270 Lys Arg Leu Lys Asp Gly Gly Ile Ala Leu Thr Asp Tyr Ala Phe Ser 275 280 285 Thr Ile Phe Val Asp Pro Pro Arg Ala Gly Ile Asp Glu Glu Thr Leu 290 295 300 Lys Leu Val Ser Gin Phe Asp Asn Ile Ile Tyr Ile Ser Cys Asn Pro 305 310 315 320 Glu Thr Leu Arg Ala Asn Leu Asp Thr Leu Ala Glu Thr His Ala Val 325 330 335 Glu Arg Ala Ala Leu Phe Asp Gin Phe Pro Phe Thr His His Ile Glu 340 345 350 Ser Gly Val Leu Leu Lys Lys Lys Ile Leu

355 360 <210> 195 <211> 2145 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(2145) <400> 195 atg aat acc cca ttg ttc cgt ctc age ctg ctc tcg ctt acc ctg gcg 48

Met Asn Thr Pro Leu Phe Arg Leu Ser Leu Leu Ser Leu Thr Leu Ala

1 5 377 10 15

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• · • ···· · · · · · • · ··· · · · · »««· «*«« ** ··· ·· ··

WO 01/85772 PCT/GB01/02003

gca ggt ttt gcc cat His gcg Ala gca Ala gaa aat aat gcc aag gtc gta ctg gat 96 Ala Gly Phe Ala 20 Glu Asn 25 Asn Ala Lys Val Val 30 Leu Asp acc gtt acc gta aaa ggc gac cgc caa ggc age aaa atc cgt acc aac 144 Thr Val Thr Val Lys Gly Asp Arg Gin Gly Ser Lys Ile Arg Thr Asn 35 40 45 atc gtt acg ctg caa caa aaa gac gaa age acc gca acc gat atg cgc 192 Xle Val Thr Leu Gin Gin Lys Asp Glu Ser Thr Ala Thr Asp Met Arg 50 55 60 gaa ctc tta aaa gaa gag ccc tcc atc gat ttc ggc ggc ggc aac ggc 240 Glu Leu Leu Lys Glu Glu Pro Sér Ile Asp Phe Gly Gly Gly Asn Gly 65 70 75 80 acg tcc caa ttc ctg acg ctg cgc ggc atg ggt caa aac tet gtc gac 288 Thr Ser Gin Phe Leu Thr Leu Arg Gly Met Gly Gin Asn Ser Val Asp 85 90 95 atc aag gtg gac aac gcc tat tcc gac age caa atc ctt tac cac caa 336 Xle Lys Val Asp Asn Ala Tyr Ser Asp Ser Gin Ile Leu Tyr His Gin 100 105 110 ggc aga ttt att gtc gat ccc get ttg gtt aaa gtc gtt tcc gta caa 384 Gly Arg Phe Ile Val Asp Pro Ala Leu Val Lys Val Val Ser Val Gin 115 120 125 aaa ggc gcg ggt tcc gcc tet gcc ggt atc ggc gcg acc aac ggc gcg 432 Lys Gly Ala Gly Ser Ala Ser Ala Gly Ile Gly Ala Thr Asn Gly Ala 130 135 140 atc atc acc aaa acc gtc gat gcc caa gac ctg ctc aaa ggc ttg gat 480 Ile Ile Thr Lys Thr Val Asp Ala Gin Asp Leu Leu Lys Gly Leu Asp 145 150 155 160 aaa aac tgg ggc gtg cgc ctc aac age ggc ttt gcc age aac gaa ggc 528 Lys Asn Trp Gly Val Arg Leu Asn Ser Gly Phe Ala Ser Asn Glu Gly 165 - - 170 175 gta age tac ggc gca age gta ttc ggg aaa gag ggc aac ttc gac ggc 576 Val Ser Tyr Gly Ala Ser Val Phe Gly Lys Glu Gly Asn Phe Asp Gly 180 185 190 ttg ttc tet tac aac cgc aac aat gaa aaa gat tac gaa gca ggt aaa 624 Leu Phe Ser Tyr Asn Arg Asn Asn Glu Lys Asp Tyr Glu Ala Gly Lys 195 200 205

378 • · · ·

WO 01/85772

PCT/GBO1/02003

ggc ttc Gly Phe 210 cgt aat aat ttc aac ggc ggč aaa acc gta ccg tac agc gcg 672 Arg Asn Asn Phe Asn 215 Gly Gly Lys Thr Val Pro 220 Tyr Ser Ala ctg gac aaa cgc agc tac ctc gcc aaa atc gga aca agc ttc ggc gac 720 Leu Asp Lys Arg Ser Tyr Leu Ala Lys llě Gly Thr Ser Phe Gly Asp 225 230 235 240 ggc gac cac cgc atc gta ttg agc cat atg aaa gac cag cac cgg ggc 768 Gly Asp His Arg Ile Val Leu Ser His Met Lys Asp Gin His Arg Gly 245 250 255 atc cgt acc gtc cgt gaa gaa ttt acc gtc ggc ggc gat aaa gag ega 816 Ile Arg Thr Val Arg Glu Glu Phe Thr Val Gly Gly Asp Lys Glu Arg 260 265 270 ata agt atg gaa cgc caa gcc cct gCt tac cgc gaa acc aca caa tcc 864 Ile Ser Met Glu Arg Gin Ala Pro Ala Tyr Arg Glu Thr Thr Gin Ser 275 280 285 aac acc aat ttg gcg tac acg ggt aaa aac ctg ggc ttt gtc gaa aaa 912 Asn Thr Asn Leu Ala Tyr Thr Gly Lys Asn Leu Gly Phe Val Glu Lys 290 295 300 ctg gat gcc aac gcc tat gtg ttg gaa aaa gáa cgc tat tcc gcc gat 960 Leu Asp Ala Asn Ala Tyr Val Leu Glu Lys Glu Arg Ťyr Ser Ala Asp 305 310 315 320 gac agc ggc acc ggt tac gca ggc aat gta aaa ggc ccc aac cat ácc 1008 Asp Ser Gly Thr Gly Tyr Ala Gly Asn Val Lys Gly Pro Asn His Thr 325 330 335 caa atc acc act cgg ggt atg aac ttc aac ttc gac agc cgc ctt gcc 1056 Gin Ile Thr Thr Arg Gly Met Asn Phe Asn Phe Asp Ser Arg Leu Ala 340 345 350 gaa caa acc ctg ctg aaa tac ggt atc aac tac cgc cat cag gaa atc 1104 Glu Gin Thr Leu Leu Lys Tyr Gly Ile Asn Tyr Arg His Gin Glu Ile 355 4 360 365 aaa ccg caa gcg ttt ttg aat tca caa ttt aaa att gaa gat aaa gaa 1152 Lys Pro Gin Ala Phe Leu Asn Ser Gin Phe Lys Ile Glu Asp Lys Glu 370 375 380 aaa gca act gat gaa gag aaa aat aag aac cgt gaa aat gaa aaa att 1200 Lys Ala Thr Asp Glu Glu Lys Asn Lys Asn Arg Glu Asn Glu Lys Ile 385 390 395 400

379 • · ···· • 0

PCT/GB01/02003

WO 01/85772 gcc aaa gcc tac cgt ctg acc aac ccg Ala Lys Ala Tyr Arg 405 Leu Thr Asn Pro tat atc gaa gcc att cac gag att gac Tyr Ile Glu Ala 420 Ile His Glu Ile Asp 425 ctg cgt tac gac cgc ttc aag gtg aaa Leu Arg Tyr 435 Asp Arg Phe Lys Val 440 Lys tea agc aac aac ctt aac ccg agt ttc Ser Ser 450 Asn Asn Leu Asn Pro 455 Ser Phe gaa cac tgg agc ttc agc gcg agc cac GlU 465 His Trp Ser Phe Ser 470 Ala Ser His cgc ctg tat gac gcg ctg caa acc cac Arg Leu Tyr Asp Ala 485 Leu Gin Thr His att gcc gac ggc acg aaa gcc gaa cgc Ile Ala Asp Gly 500 Thr Lys Ala Glu Arg 505 ttc aac tac aac gac ggc acg ttt gcc Phe Asn Tyr 515 Asn Asp Gly Thr Phe 520 Ala cag acc atc aaa gac gcg ctt gcc aat Gin Thr 530 Ile Lys Asp Ala Leu 535 Ala Asn gtc gcc gtc cgt gaa gcc gtc aat gcc Val 545 Ala Val Arg Glu Ala 550, Val Asn Ala tac gaa ttg ggc gcg tcc tac cgc acc Tyr Glu Leu Gly Ala 565 Ser Tyr Arg Thr ggc gta agc cac agc aaa ccg cgc ttt Gly Val Ser His Ser Lys Pro Arg Phe

580 585

acc aaa acc gat acc ggc gcg 1248 Thr 410 Lys Thr Asp Thr Gly 415 Ala ggc ttt acc ctg acc ggc ggg 1296 Gly Phe Thr Leu Thr 430 Gly Gly acc cac gac ggc aaa acc gtt 1344 Thr His Asp Gly 445 Lys Thr Val ggc gtg att tgg cag ccg cac 1392 Gly Val Ile 460 Trp Gin Pro His aac tac gcc agc cgc agc ccg 1440 Asn Tyr 475 Ala Ser Arg Ser Pro 480 ggc aaa cgc ggc atc atc tcg 1488 Gly 490 Lys Arg Gly Ile Ile 495 Ser gcg cgc aat acc gaa atc ggc 1536 Ala Arg Asn Thr Glu 510 Ile Gly gca aac ggc agc tac ttc tgg 1584 Ala Asn Gly Ser 525 Tyr Phe Trp ccg caa aac cgc cac gac tet 1632 Pro Gin Asn 540 Arg His Asp Ser ggt tac atc aaa aac cac ggt 1680 Gly Tyr 555 . Ile Lys Asn His Gly 560 ggc ggc ctg act gcc aaa gtc 1728 Gly 570 Gly Leu Thr Ala Lys 575 Val tac gat acg cac aaa gac aag 1776 Tyr Asp Thr His Lys 590 Asp Lys

380

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• · · eeβe ···« «β ee

WO 01/85772

PCT/GBO1/02003

ctg ttg agc gcg Ala aat Asn cct Pro gaa Glu ttt ggc gca caa gtc ggc cgc act tgg 1824 Leu Leu Ser 595 Phe Gly 600 Ala Gin Val Gly Arg 605 Thr Trp acg gct tcc ctt gcc tac cgc ttc caa aac ccg aat ctg gaa atc ggc 1872 Thr Ala Ser Leu Ala Tyr Arg Phe Gin Asn Pro Asn Leu Glu Ile Gly 610 615 620 tgg cgc ggc cgt tat gtt caa aaa gcc gtg ggt tcg ata ttg gtg gca 1920 Trp Arg Gly Arg Tyr Val Gin Lys Ala Val Gly Ser Ile Leu Val Ala 625 630 635 640 ggt caa aaa gac cgc aac ggc aaa ttg gaa aac gtt gta cgc aaa ggt 1968 Gly Gin Lys Asp Arg Asn Gly Lys Leu Glu Asn Val Val Arg Lys Gly 645 650 655 ttc ggt gtg aác gat gtc ttc gcc aac tgg aaa ccg ctg ggc aaa gac 2016 Phe Gly Val Asn Asp Val Phe Ala Asn Trp Lys Pro Leu Gly Lys Asp 660 665 670 acg ctc aat gtt aat ctt tcg gtt aac aac gtg ttc aac acg ttc tac 2064 Thr Leu Asn Val Asn Leu Ser Val Asn Asn Val Phe Asn Thr Phe Tyr 675 680 685 tat ccg cac agc caa ega tgg acc aat acc ctg ccg ggc gtg gga cgt 2112 Tyr Pro His Ser Gin Arg Trp Thr Asn Thr Leu Pro Gly Val Gly Arg 690 695 700 gat gta cgc ttg ggc gtg aac tac aag ttc taa 2145 Asp Val Arg Leu Gly Val Asn Tyr Lys Phe

705

710

715 <210> 196 <211> 714 <212> PRT <213> Neisseria meningitidis <400> 196

Met Asn Thr Pro Leu Phe 'Arg Leu Ser Leu Leu Ser Leu Thr Leu Ala 1 5 10 15

Ala Gly Phe Ala His Ala Ala Glu Asn Asn Ala Lys Val Val Leu Asp 20 25 30

Thr Val Thr Val Lys Gly Asp Arg Gin Gly Ser Lys Ile Arg Thr Asn 35 40 45

381

WO 01/85772 PCT/GBO1/02003 Zle Val Thr Leu Gin. Gin Lys Asp Glu Ser Thr Ala 50 55 60 Thr Asp Met Arg Glu Leu Leu Lys Glu Glu Pro Ser Ile Asp Phe Gly 65 70 75 Gly Gly Asn Gly 80 Thr Ser Gin Phe Leu Thr Leu Arg Gly Met Gly Gin 85 90 Asn Ser Val Asp 95 Ile Lys Val Asp Asn Ala Tyr Ser Asp Ser Gin Ile 100 105 Leu Tyr His Gin 110 Gly Arg Phe Ile Val Asp Pro Ala Leu Val Lys Val 115 120 Val Ser Val Gin 125 Lys Gly Ala Gly Ser Ala Ser Ala Gly Ile Gly Ala 130 135 140 Thr Asn Gly Ala Ile Ile Thr Lys Thr Val Asp Ala Gin Asp Leu Leu 145 150 155 Lys Gly Leu Asp 160 Lys Asn Trp Gly Val Arg Leu Asn Ser Gly Phe Ala 165 170 Ser Asn Glu Gly 175 Val Ser Tyr Gly Ala Ser Val Phe Gly Lys Glu Gly 180 185 Asn Phe Asp Gly 190 Leu Phe Ser Tyr Asn Arg Asn Asn Glu Lys Asp Tyr 195 200 Glu Ala Gly Lys 205 Gly Phe Arg Asn Asn Phe Asn Gly Gly Lys Thr Val 210 215 220 Pro Tyr Ser Ala Leu Asp Lys Arg Ser Tyr Leu Ala Lys Ile Gly Thr 225 230 235 Ser Phe Gly Asp 240 Gly Asp His Arg Ile Val Leu Ser His Met Lys Asp 245 250 Gin His Arg Gly 255 Ile Arg Thr Val Arg Glu Glu Phe Thr Val Gly Gly 260 265 Asp Lys Glu Arg 270 Ile Ser Met Glu Arg Gin Ala Pro Ala Tyr Arg Glu 275 280 Thr Thr Gin Ser 285 Asn Thr Asn Leu Ala Tyr Thr Gly Lys Asn Leu Gly 290 295 300 Phe Val Glu Lys

382 • « β e se a

WO 01/85772

PCT/GBO1/02003

Leu 305 Asp Ala Asn Ala Tyr 310 Val Leu Glu Lys Glu 315 Arg Tyr Ser Ala Asp 320 Asp Ser Gly Thr Gly 325 Tyr Ala Gly Asn Val .330 Lys Gly Pro Asn His 335 Thr Gin Ile Thr Thr 340 Arg Gly Met Asn Phe 345 Asn Phe Asp Ser Arg 350 Leu Ala Glu Gin Thr 355 Leu Leu Lys Tyr Gly 360 Ile Asn Tyr Arg His 365 Gin Glu Ile Lys Pro 370 Gin Ala Phe Leu Asn 375 Ser Gin Phe Lys Ile 380 Glu Asp Lys Glu Lys 385 Ala Thr Asp Glu Glu 390 Lys Asn Lys Asn Arg 395 Glu Asn Glu Lys Ile 400 Ala Lys Ala Tyr Arg 405 Leu Thr Asn Pro Thr 410 Lys Thr Asp Thr Gly 415 Ala Tyr Ile Glu Ala 420 Ile His Glu Ile Asp 425 Gly Phe Thr Leu Thr 430 Gly Gly Leu Arg Tyr 435 Asp Arg Phe Lys Val 440 Lys Thr His Asp Gly 445 Lys Thr Val Ser Ser 450 Asn Asn Leu Asn Pro 455 Ser Phe Gly Val Ile 460 Trp Gin Pro His Glu 465 His Trp Ser Phe Ser 470 Ala Ser His Asn Tyr 475 Ala Ser Arg Ser Pro 480 Arg Leu Tyr Asp Ala 485 Leu Gin Thr His Gly 490 Lys Arg Gly Ile Ile 495 Ser Ile Ala Asp Gly 500 Thr Lys Ala Glu Arg 505 Ala Arg Asn Thr Glu 510 Ile Gly Phe Asn Tyr 515 Asn Asp Gly *Thr Phe 520 Ala Ala Asn Gly Ser 525 Tyr Phe Trp Gin Thr 530 Ile Lys Asp Ala Leu 535 Ala Asn Pro Gin Asn 540 Arg His Asp Ser Val 545 Ala Val Arg Glu Ala 550 Val Asn Ala Gly Tyr 555 Ile Lys Asn His Gly 560

383 eeeo esee

WO 01/85772 PCT/GB01/02003 Tyr Glu Leu Gly Ala Ser Tyr Arg Thr Gly Gly Leu Thr Ala Lys Val 565 570 575 Gly Val Ser His Ser Lys Pro Arg Phe Tyr Asp Thr His Lys Asp Lys 580 585 590 Leu Leu Ser Ala Asn Pro Glu Phe Gly Ala Gin Val Gly Arg Thr Trp 595 600 605 Thr Ala Ser Leu Ala Tyr Arg Phe Gin Asn Pro Asn Leu Glu Ile Gly 610 615 620 Trp Arg Gly Arg Tyr Val Gin Lys Ala Val Gly Ser Ile Leu Val Ala 625 630 635 640 Gly Gin Lys Asp Arg Asn Gly Lys Leu Glu Asn Val Val Arg Lys Gly 645 650 655 Phe Gly Val Asn Asp Val Phe Ala Asn Trp Lys Pro Leu Gly Lys Asp 660 665 670 Thr Leu Asn Val Asn Leu Ser Val Asn Asn Val Phe Asn Thr Phe Tyr 675 680 685 Tyr Pro His Ser Gin Arg Trp Thr Asn Thr Leu Pro Gly Val Gly Arg 690 695 700 Asp Val Arg Leu Gly Val Asn Tyr Lys Phe 705 710

<210> 197 <211> 2625 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(2625) <400> 197 atg aaa acc tcc gaa ctg cgc caa aaa ttc cta aaa ttt ttt gaa acc 48

Met Lys Thr Ser Glu Leu Arg Gin Lys Phe Leu Lys Phe Phe Glu Thr

15 10 15 aaa ggc cac acc gtc gtc cgc tet tcc agc ctc gtg ccg cac gac gac 96

384

WO 01/85772 PCT/GB01/02003 Lys Gly His Thr Val Val Arg Ser Ser Ser Leu Val Pro His Asp Asp 20 25 30 ccg acc ctg ctg ttt acc aac gcg ggc atg aac cag ttt aaa gac gta 144 Pro Thr Leu Leu Phe Thr Asn Ala Gly Met Asn Gin Phe Lys Asp Val 35 40 45 ttc tta ggt ttc gac aaa cgc ccg tac age cgc gcc acc acc gcg caa 192 Phe Leu Gly Phe Asp Lys Arg Pro Tyr Ser Arg Ala Thr Thr Ala Gin 50 55 60 aaa tgc gta cgc gca ggc ggc aaa cac aac gac ttg gaa aac gtc ggc 240 Lys Cys Val Arg Ala Gly Gly Lys His Asn Asp Leu Glu Asn Val Gly 65 70 75 80 tac acc gcc cgc cac cac acc ttc ttt gaa atg atg ggc aac ttc tcc 288 Tyr Thr Ala Arg His His Thr Phe Phe Glu Met Met Gly Asn Phe Ser 85 90 95 ttc ggc gac tac ttc aaa cgc gac gcc atc cac ttc gct tgg gaa ttt 336 Phe Gly Asp Tyr Phe Lys Arg Asp Ala Ile His Phe Ala Trp Glu Phe 100 105 110 ctg act tcc ccc gaa tgg ctc aac atc cct aaa gac aaa ctg ttg gcg 384 Leu Thr Ser Pro Glu Trp Leu Asn Ile Pro Lys Asp Lys Leu Leu Ala 115 120 125 acc gtt tac gcg gaa gac gac gaa gcc tac aac atc tgg ttg aac gaa 432 Thr Val Tyr Ala Glu Asp Asp Glu Ala Tyr Asn Ile Trp Leu Asn Glu 130 135 140 atc ggt atg ccg tcc gag cgc atc gtc cgc atc ggc gac aac aaa ggc 480 Ile Gly Met Pro Ser Glu Arg Ile Val Arg Ile Gly Asp Asn Lys Gly 145 150 155 160 gcg aaa tac gca tcc gac aac ttc tgg caa atg ggc gac acc ggc cct 528 Ala Lys Tyr Ala Ser Asp Asn Phe Trp Gin Met Gly Asp Thr Gly Pro 165 170 175 tgc ggc ccc tgc tcc gaa att ttc tac gac cac ggc gaa gaa atc tgg 576 Cys Gly Pro Cys Ser Glu Ile Phe Tyr Asp His Gly Glu Glu Ile Trp 180 185 190 ggc ggc att ccc ggc agt ccc gaa gaa gac ggc gac cgc tgg atc gaa 624 Gly Gly Ile Pro Gly Ser Pro Glu Glu Asp Gly Asp Arg Trp Ile Glu 195 200 205 att tgg aac tgc gta ttt atg cag ttc aac cgc gac gaa caa ggc aat 672

385

WO 01/85772 PCT/GBO1/02003 Ile Trp Asn Cys Val Phe Met Gin Phe Asn Arg Asp Glu Gin Gly Asn 210 215 220 atg aac ccg ctt ccc aaa cct tcc gtc gat acc ggt atg ggc ttg gaa 720 Met Asn Pro Leu Pro Lys Pro Ser Val Asp Thr Gly Met Gly Leu Glu 225 230 235 240 cgc ata gcc gcc gtc atg cag cat gtt cac age aac tac gaa atc gac 768 Arg Ile Ala Ala Val Met Gin His Val His ser Asn Tyr Glu Ile Asp 245 250 255 ttg ttc caa gac ctg ctc aaa gcc gtt gcc cgc gaa acc ggc gcg ccg 816 Leu Phe Gin Asp Leu Leu Lys Ala Val Ala Arg Glu Thr Gly Ala Pro 260 265 270 ttc aga atg gaa gaa ccc age ctg aaa gtc atc gcc gac cac atc cgc 864 Phe Arg Met Glu Glu Pro Ser Leu Lys Val Ile Ala Asp His Ile Arg 275 280 285 tcc tgc tcg ttc ctg att gca gac ggc gtc ttg cct tcc aac gaa ggc 912 Ser Cys Ser Phe Leu Ile Ala Asp Gly Val Leu Pro Ser Asn Glu Gly 290 295 300 cgc ggc tac gta ttg cgc cgc att atc cgc cgc gcc gtg cgc cac ggt 960 Arg Gly Tyr Val Leu Arg Arg Ile Ile Arg Arg Ala Val Arg His Gly 305 310 315 320 tac aaa ctg ggt caa age aaa ccg ttc ttc cac aaa ctc gtt gcc gat 1008 Tyr Lys Leu Gly Gin Ser Lys Pro Phe Phe His Lys Leu Val Ala Asp 325 330 335 ttg gtc aaa gag atg ggc ggt gcc tac cct gaa ttg aaa gaa aaa caa 1056 Leu Val Lys Glu Met Gly Gly Ala Tyr Pro Glu Leu Lys Glu Lys Gin 340 345 350 gcc caa atc gaa gaa gca ttg aaa aac gaa gaa age cgt ttt gcc caa 1104 Ala Gin Ile Glu Glu Ala Leu Lys Asn Glu Glu Ser Arg Phe Ala Gin 355 360 365 acg ctg gaa acc ggt atg,.get ttg ttg gaa aac gcg ctg gtc aaa ggc 1152 Thr Leu Glu Thr Gly Met Ala Leu Leu Glu Asn Ala Leu Val Lys Gly 370 375 380 ggc aaa aca ctc ggc ggc gaa atc atc ttc aaa ctc tac gat acc tac 1200 Gly Lys Thr Leu Gly Gly Glu Ile Ile Phe Lys Leu Tyr Asp Thr Tyr 385 390 395 400 ggt ttc cca tac gac ttg act gcc gac atc tgc cgc gaa cgc aat atc 1248

386

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WO 01/85772 PCT/GBO1/02003 Gly Phe Pro Tyr Asp Leu Thr Ala Asp Ile Cys Arg Glu Arg Asn Ile 405 410 415 gaa ccg gac gaa gca ggc ttc gag cgc gaa atg gaa gcc caa cgc gca 1296 Glu Pro Asp Glu Ala Gly Phe Glu Arg Glu Met Glu Ala Gin Arg Ala 420 425 430 cgc gca cgc gcc gcc caa agc ttc aaa gcc aac gcc caa ctg cct tat 1344 Arg Ala Arg Ala Ala Gin Ser Phe Lys Ala Asn Ala Gin Leu Pro Tyr 435 440 445 gac ggt caa gac acc gag ttt aaa ggt tat agc gaa cgc caa acc gaa 1392 Asp Gly Gin Asp Thr Glu Phe Lys Gly Tyr Ser Glu Arg Gin Thr Glu 450 455 460 tcc aaa gtc ctc gcc ctc tac aaa gac ggc gag caa gtc aac gaa ttg 1440 Ser Lys Val Leu Ala Leu Tyr Lys Asp Gly Glu Gin Val Asn Glu Leu 465 470 475 480 aac gaa ggc gac agc ggc gca gtc gtc atc gac ttt acc ccg ttc tat 1488 Asn Glu Gly Asp Ser Gly Ala Val Val Ile Asp Phe Thr Pro Phe Tyr 485 490 495 gca gaa tcc ggc ggc caa gtc ggc gat gtc ggc tat atc ttc tea ggc 1536 Ala Glu Ser Gly Gly Gin Val Gly Asp Val Gly Tyr Ile Phe ser Gly 500 505 510 gaa aac cgc ttt gaa gta cgc gat acc caa aaa atc aaa gcg gcc gta 1584 Glu Asn Arg Phe Glu Val Arg Asp Thr Gin Lys Ile Lys Ala Ala Val 515 520 525 ttc ggt caa ttc ggc gta caa act tea ggc cgt ctg aaa gtc ggc gac 1632 Phe Gly Gin Phe Gly Val Gin Thr Ser Gly Arg Leu Lys Val Gly Asp 530 535 540 agc gtt acc gcc aaa gtg gac gac gaa atc cgc aat gcc aat atg cgc 1680 Ser Val Thr Ala Lys Val Asp Asp Glu Ile Arg Asn Ala Asn Met Arg 545 550 555 560 aac cac agc gca acc cac ttg atg cac aaa gcc ctg cgc gat gta ttg 1728 Asn His Ser Ala Thr His ' Leu Met His Lys Ala Leu Arg Asp Val Leu 565 570 575 ggc aga cac gtc gaa caa aaa ggc tet ttg gtt acc gcc gaa tcc acc 1776 Gly Arg His Val Glu Gin Lys Gly Ser Leu Val Thr Ala Glu Ser Thr 580 585 590 cgt ttc gac att tcc cat ccc caa gcg gta act gcc gaa gaa att gcc 1824

387

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999

WO 01/85772

PCT/GBO1/02003

Arg Phe Asp 595 Ile Ser His Pro Gin 600 Ala Val Thr Ala Glu 605 Glu Ile-Ala gaa gta gaa cgc cgc gtc aac gaa gcc att ttg gcg aac gtt gcc gtc 1872 Glu Val 610 Glu Arg Arg Val Asn 615 GlU Ala Ile Leu Ala 620 Asn Val Ala Val aat gca gcc att atg age atg gaa gac gcg caa aaa acc ggc gcg atg 1920 Asn 625 Ala Ala Ile Met Ser 630 Met Glu Asp Ala Gin 635 Lys Thr Gly Ala Met 640 atg ctc ttc ggc gaa aaa tac ggc gaa gaa gtg cgc gta ctg caa atg 1968 Met Leu Phe Gly Glu 645 Lys Tyr Gly Glu Glu 650 Val Arg Val Leu Gin 655 Met ggc ggt ttc tet acc gaa ttg tgc ggc ggc aca cac gtt tca cgc acc 2016 Gly Gly Phe Ser 660 Thr Glu Leu Cys Gly Gly 665 Thr His Val Ser 670 Arg Thr ggc gac atc ggc ctc ttc aaa atc atc age gaa ggc ggt att gcc gca 2064 Gly Asp Ile 675 Gly Leu Phe Lys Ile 680 Ile Ser Glu Gly Gly 685 Ile Ala Ala ggc gtg cgc cgt atc gaa gcc atc acc ggc ctg aac gca ctc aaa tgg 2112 Gly Val 690 Arg Arg Ile Glu Ala 695 Ile Thr Gly Leu Asn 700 Ala Leu Lys Trp gcg caa gag caa gag cgt ttg gtg aaa gac att att gcc gaa acc aaa 2160 Ala 705 Gin Glu Gin Glu Arg 710 Leu Val Lys Asp Ile 715 Ile Ala Glu Thr Lys 720 gcc caa acc gaa aaa gac gta ctg gca aaa atc caa gca ggc gcg gca 2208 Ala Gin Thr Glu Lys 725 Asp Val Leu Ala Lys 730 Ile Gin Ala Gly Ala 735 Ala cac gcc aaa gca ttg gaa aaa gaa ttg gca cgc gcc aaa gcc gaa ctc 2256 His Ala Lys Ala 740 Leu Glu Lys Glu Leu 745 Ala Arg Ala Lys Ala 750 Glu Leu gcc gtc cac gca ggc gcc - aaa ctc ttg gac gat gca aaa gac ttg ggc 2304 Ala Val His 755 Ala Gly Ala Lys Leu 760 Leu Asp Asp Ala Lys 765 Asp Leu Gly gca gcc aaa ctc gtt gcc gcc caa atc gaa gcc gac gca gcc gcc ctg 2352 Ala Ala 770 Lys Leu Val Ala Ala 775 Gin Ile Glu Ala Asp 780 Ala Ala Ala Leu cgc gaa atc gtt acc gat tta acc ggt aaa tcc gac aac gcc gtg att 2400

388

WO 01/85772 PCT/GB01/02003 Arg Glu Ile Val Thr Asp Leu Thr Gly Lys Ser Asp Asn Ala Val Xle 785 790 795 800 ctt tta gcg gca gta aac gac ggc aaa gtc tcc ctg tgc gcc ggc gta 2448 Leu Leu Ala Ala Val Asn Asp Gly Lys Val Ser Leu Cys Ala Gly Val 805 810 815 tcc aaa ccg ttg acc ggc aaa gtg aaa gca ggc gat ctg gtt aaa ttt 2496 Ser Lys Pro Leu Thr Gly Lys Val Lys Ala Gly Asp Leu Val Lys Phe 820 825 830 gca gcc gaa caa gtc ggc ggc aaa ggc ggc ggc aga cca gat ttg gcg 2544 Ala Ala Glu Gin Val Gly Gly Lys Gly Gly Gly Arg Pro Asp Leu Ala 835 840 845 caa gcc ggc ggc acg gat gcc gac aaa ttg ccc gcc gtg ttg gat agc 2592 Gin Ala Gly Gly Thr Asp Ala Ásp Lys Leu Pro Ala Val Leu Asp Ser 850 855 860 gtg aaa gac tgg gtc ggc gcg aag ctg gtt tga .2625 Val Lys Asp Trp Val Gly Ala Lys Leu Val 865 870 875 <210> 198 <211> 874 <212> PRT <213> Neisseria meningitidis <400> 198 Met Lys Thr Ser Glu Leu Arg Gin Lys Phe Leu Lys Phe Phe Glu Thr ^{1 5} 10 15 Lys Gly His Thr Val Val Arg Ser Ser Ser Leu Val Pro His Asp Asp 20 25 30 Pro Thr Leu Leu Phe Thr Asn Ala Gly Met Asn Gin Phe Lys Asp Val 35 40 45 Phe Leu Gly Phe Asp Lys Arg Pro Tyr Ser Arg Ala Thr Thr Ala Gin 50 ’ 55 60 Lys Cys Val Arg Ala Gly Gly Lys His Asn Asp Leu Glu Asn Val Gly 65 70 75 80 Tyr Thr Ala Arg His His Thr Phe Phe Glu Met Met Gly Asn Phe Ser 85 90 95

389

WO 01/85772

PCT/GB01/02003

Phe Gly Asp Tyr Phe Lys Arg Asp Ala Ile His Phe Ala Trp Glu Phe 100 105 110 Leu Thr Ser 115 Pro Glu Trp Leu Asn Ile Pro Lys Asp Lys 120 125 Leu Leu Ala Thr Val Tyr 130 Ala Glu Asp Asp Glu Ala Tyr Asn Ile Trp 135 140 Leu Asn Glu Ile 145 Gly Met Pro Ser Glu Arg Ile Val Arg Ile Gly Asp 150 155 Asn Lys Gly 160 Ala Lys Tyr Ala Ser 165 Asp Asn Phe Trp Gin Met Gly Asp 170 Thr Gly Pro 175 Cys Gly Pro Čys Ser 180 Glu Ile Phe Tyr Asp His Gly Glu 185 Glu Ile Trp 190 Gly Gly Ile 195 Pro Gly Ser Pro Glu Glu Asp Gly Asp Arg 200 205 Trp Ile Glu Ile Trp Asn 210 Cys Val Phe Met Gin Phe Asn Arg Asp Glu 215 220 Gin Gly Asn Met 225 Asn Pro Leu Pro Lys Pro Ser Val Asp Thr Gly Met 230 235 Gly Leu Glu 240 Arg Ile Ala Ala Val 245 Met Gin His Val His Ser Asn Tyr 250 Glu Ile Asp 255 Leu Phe Gin Asp Leu 260 Leu Lys Ala Val Ala Arg Glu Thr 265 Gly. Ala Pro 270 Phe Arg Met 275 Glu Glu Pro Ser Leu Lys Val Ile Ala Asp 280 285 His Ile Arg Ser Cys Ser 290 Phe Leu Ile Ala Asp Gly Val Leu Pro Ser 295 300 Asn Glu Gly Arg 305 Gly Tyr Val Leu Arg,Arg Ile Ile Arg Arg Ala Val 310 315 Arg His Gly 320 Tyr Lys Leu Gly Gin 325 Ser Lys Pro Phe Phe His Lys Leu 330 Val Ala Asp 335 Leu Val Lys Glu Met Gly Gly Ala Tyr Pro Glu Leu Lys Glu Lys Gin

340 345 350

390 ·· ϊ c e e e e« í frfr

PCT/GB01/02003

WO 01/85772

Ala Gin Ile 355 Glu Glu Ala Leu Lys 360 Asn Glu Glu Ser Arg 365 Phe Ala Gin Thr Leu 370 Glu Thr Gly Met Ala 375 Leu Leu Glu Asn Ala 380 Leu Val Lys Gly Gly 395 Lys Thr Leu Gly Gly 390 Glu Ile Ile Phe Lys 395 Leu Tyr Asp Thr Tyr 400 Gly Phe Pro Tyr Asp 405 Leu Thr Ala Asp Ile 410 Cys Arg Glu Arg Asn 415 Ile Glu Pro Asp Glu 420 Ala Gly Phe Glu Arg 425 Glu Met Glu Ala Gin 430 Arg Ala Arg Ala Arg 435 Ala Ala Gin Ser Phe 440 Lys Ala Asn Ala Gin 445 Leu Pro Tyr Asp Gly 450 Gin Asp Thr Glu Phe 455 Lys Gly Tyr Ser Glu 460 Arg Gin Thr Glu Ser 465 Lys Val Leu Ala Leu 470 Tyr Lys Asp Gly Glu 475 Gin Val Asn Glu Leu 480 Asn Glu Gly Asp Ser 485 Gly Ala Val Val Ile 490 Asp Phe Thr Pro Phe 495 Tyr Ala Glu Ser Gly 500 Gly Gin Val Gly Asp 505 Val Gly Tyr Ile Phe 510 Ser Gly Glu Asn Arg 515 Phe Glu Val Arg Asp 520 Thr Gin Lys Ile Lys 525 Ala Ala Val Phe Gly 530 Gin Phe Gly Val Gin 535 Thr Ser Gly Arg Leu 540 Lys Val Gly Asp Ser 545 Val Thr Ala Lys Val 550 Asp Asp Glu Ile Arg 555 Asn Ala Asn Met Arg 560 Asn His Ser Ala Thr 565 His Leu Met His Lys 570 Ala Leu Arg Asp Val 575 Leu Gly Arg His Val 580 Glu Gin Lys Gly Ser 585 Leu Val Thr Ala Glu 590 Ser Thr Arg Phe Asp Ile Ser His Pro Gin Ala Val Thr Ala Glu Glu Ile Ala

595 600 605

391 ·· eee β®

PCT/GBO1/02003

WO 01/85772

Glu Val 610 Glu Arg Arg Val Asn 615 Glu Ala Ile Leu Ala 620 Asn Val Ala Val Asn 625 Ala Ala Ile Met Ser 630 Met Glu Asp Ala Gin 635 Lys Thr Gly Ala Met 640 Met Leu Phe Gly Glu 645 Lys Tyr Gly Glu Glu 650 Val Arg Val Leu Gin 655 Met Gly Gly Phe Ser 660 Thr Glu Leu Cys Gly 665 Gly Thr His Val Ser 670 Arg Thr Gly Asp Ile 675 Gly Leu Phe Lys Ile 680 Ile Ser Glu Gly Gly 685 Ile Ala Ala Gly Val 690 Arg Arg Ile Glu Ala 695 Ile Thr Gly Leu Asn 700 Ala Leu Lys Trp Ala 705 Gin Glu Gin Glu Arg 710 Leu Val Lys Asp Ile 715 Ile Ala Glu Thr Lys 720 Ala Gin Thr Glu Lys 725 Asp Val Leu Ala Lys 730 Ile Gin Ala Gly Ala 735 Ala His Ala Lys Ala 740 Leu Glu Lys Glu Leu 745 Ala Arg Ala Lys Ala 750 Glu Leu Ala Val HÍS 755 Ala Gly Ala Lys Leu 760 Leu Asp Asp Ala Lys 765 Asp Leu Gly Ala Ala 770 Lys Leu Val Ala Ala 775 Gin Ile Glu Ala Asp 780 Ala Ala Ala Leu Arg 785 Glu Ile Val Thr Asp 790 Leu Thr Gly Lys Ser 795 Asp Asn Ala Val Ile 800 Leu Leu Ala Ala Val 805 Asn Asp Gly Lys Val 810 Ser Leu Cys Ala Gly 815 Val Ser Lys Pro Leu Thr Gly_xLys Val Lys Ala Gly Asp Leu Val Lys Phe

Ala Ala 820 Glu Gin Val Gly Gly Lys 825 830 Asp Leu Ala Gly Gly Gly Arg Pro 845 835 840 Gin Ala Gly Gly Thr Asp Ala Asp Lys Leu Pro Ala Val Leu Asp Ser 850 855 860

392

WO 01/85772 PCT/GBO1/02003

Val Lys Asp Trp Val Gly Ala Lys Leu Val

865 870 <210> 199 <211> 207 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(207) <400> 199

gtg Val l caa Gin aga ggc cgt Arg 5 ctg aaa Leu Lys ggt Gly ctc gtt tgc cgt agg ttg ggt cgc Leu Gly Arg 15 48 Arg Gly Leu Val 10 Cys Arg Arg gac cca aca aat ttt gtg aag tat aaa aat gtt ggt cat gac cca acc 96 Asp Pro Thr Asn Phe Val Lys Tyr Lys Asn Val Gly His Asp Pro Thr 20 25 30 tac ctg cct ttt tgt aca aag agg cta tet gaa agg cct tgt ttg ccg 144 Tyr Leu Pro Phe Cys Thr Lys Arg Leu Ser Glu Arg Pro Cys Leu Pro 35 40 45 tat ggt ggg tcg ega ccc age aga ttt tta tta ggg tat gac cca age 192 Tyr Gly Gly Ser Arg Pro Ser Arg Phe Leu Leu Gly Tyr Asp Pro Ser

50 55 60 tac ttg cta ega taa 207

Tyr Leu Leu Arg <210> 200 <211> 68 <212> PRT <213> Neisseria meningitidis <400> 200

Val Gin Arg Gly Arg Leu Lys Gly Leu Val Cys Arg Arg Leu Gly Arg 15 10 15

Asp Pro Thr Asn Phe Val Lys Tyr Lys Asn Val Gly His Asp Pro Thr 20 25 30

393

WO 01/85772

Tyr Leu Pro Phe Cys Thr Lys Arg Leu Ser Glu Arg Pro Cys 35 40 45

Tyr Gly Gly Ser Arg Pro Ser Arg Phe Leu Leu Gly Tyr Asp 50 55 60

Tyr Leu Leu Arg 65

PCT/GB01/02003

Leu Pro

Pro Ser <210> 201 <211> 2277 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(2277) <400> 201

atg gca caa act aca ctc aaa ccc att gtt tta tca att ctt tta Ilé Val Leu Ser Ile Leu Leu atc Ile 48 Met Ala 1 Gin Thr Thr 5 Leu Lys Pro 10 15 aac aca ccc ctc ctc gcc caa gcg cat gaa act gag caa tcg gtg gat 96 Asn Thr Pro Leu Leu Ala Gin Ala His Glu Thr Glu Gin Ser Val Asp 20 25 30 ttg gaa acg gtc age gtc gtc ggc aaa age cgt ccg cgc gcc acg tcg 144 Leu Glu Thr Val Ser Val Val Gly Lys Ser Arg Pro Arg Ala Thr Ser 35 40 45 ggg ctg ttg cac act tcg acc gcc tcc gac aaa atc atc tcc ggc gat 192 Gly Leu Leu His Thr Ser Thr Ala Ser Asp Lys Ile Ile Ser Gly Asp 50 55 60 acc ttg cgc caa aaa gcc gtc aac ttg ggc gac get tta gac ggc gta 240 Thr Leu Arg Gin Lys Ala Val Asn Leu Gly Asp Ala Leu Asp Gly Val 65 70 75 80 ccg ggc atc cac get tcg caa tac ggc ggc ggc gcg tet get ccc gtc 288 Pro Gly Ile His Ala Ser Gin Tyr Gly Gly Gly Ala Ser Ala Pro Val 85 90 95 att cgc ggt caa aca ggc agg cgg att aaa gtg ttg aac cat cac ggc 336 Ile Arg Gly Gin Thr Gly Arg Arg Ile Lys Val Leu Asn His His Gly 100 105 110

394

WO 01/85772 PCT/GB01/02003

gaa aca ggc gat atg gcg Ala gat ttt Asp Phe 120 tcg ccc gat cac gcc att atg gta Val 384 Glu Thr Gly 115 Asp Met Ser Pro Asp His Ala 125 Ile Met gat acc gcc ttg tcg caa cag gtc gaa atc ctg cgc ggg ccg gtt acg 432 Asp Thr Ala Leu Ser Gin Gin Val Glu •Ile Leu Arg Gly Pro Val Thr 130 135 140 ctc ttg tac agc tcg ggc aat gtg gcg ggg ctg gtc gat gtt gcc gat 480 Leu Leu Tyr Ser Ser Gly ASn Val Ala Gly Leu Val Asp Val Ala Asp 145 150 155 160 ggc aaa atc ccc gaa aaa atg cct gaa aac ggc gta tcg ggc gaa ctc 528 Gly Lys Ile Pro Glu Lys Met Pro Glu Asn Gly Val Ser Gly Glu Leu 165 170 175 gga ttg cgt ttg agc agc ggc aat ctg gaa aaa ctc acg tcc ggc ggc 576 Gly Leu Arg Leu Ser Ser Gly Asn Leu Glu Lys Leu Thr Ser Gly Gly 180 185 190 atc aat atc ggt ttg ggc aaa aac ttt gta ttg cac acg gaa ggg ctg 624 Ile Asn Ile Gly Leu Gly Lys Asn Phe Val Leu His Thr Glu Gly Leu 195 200 205 tac cgc aaa tcg ggg gat tac gcc gta ccg cgt tac cgc aat ctg aaa 672 Tyr Arg Lys Ser Gly Asp Tyr Ala Val Pro Arg Tyr Arg Asn Leu Lys 210 215 220 cgc ctg ccc gac agc cac gcc gat tcg caa acg ggc agc atc ggg Ctg 720 Arg Leu Pro Asp Ser His Ala Asp Ser Gin Thr Gly Ser Ile Gly Leu 225 230 235 240 tet tgg gtt ggc gaa aaa ggt ttt atc ggc gta gcg tac agc gac cgt 768 Ser Trp Val Gly Glu Lys Gly Phe Ile Gly Val Ala Tyr Ser Asp Arg 245 250 255 cgc gac caa tat ggt ctg cct gcc cac agc cac gaa tac gat gat tgc 816 Arg Asp Gin Tyr Gly Leu Pro Ala His Ser His Glu Tyr Asp Asp Cys 260 265 270 cac gcc gac atc atc tgg caa aag agc ttg att aac aaa cgc tat tta 864 His Ala Asp Ile Ile Trp Gin Lys Ser Leu Ile Asn Lys Arg Tyr Leu 275 280 285 cag ctt tat ccg cac ctg ttg acc gaa gaa gac atc gat tac gac aat 912 Gin Leu Tyr Pro His Leu Leu Thr Glu Glu Asp Ile Asp Tyr Asp Asn

290 295 300

395

4 '4 '9 c «c « · » ·· ϋ fc

4 ís β

WO 01/85772 PCT/GB01/02003

ccg ggc ttg agc tgc ggc ttc cac gac gac gat aat gca cac gca cac 960 Pro 305 Gly Leu Ser Cys Gly 310 Phe His Asp Asp Asp 315 Asn Ala His Ala His 320 acc cac agc ggc aga ccg tgg ata gac ctg cgc aac aaa cgc tac gaa 1008 Thr His Ser Gly Arg Pro Trp Ile Asp Leu Arg Asn Lys Arg Tyr Glu 325 330 335 ctc cgt gcc gaa tgg aag caa ccg ttc ccc ggt ttt gaa gcc ctg cgc 1056 Leu Arg Ala Glu Trp Lys Gin Pro Phe Pro Gly Phe Glu Ala Leu Arg 340 345 350 gta cac ctg aac cgc aac gac tac cgc cac gac gaa aaa gca ggc gat 1104 Val His Leu Asn Arg Asn Asp Tyr Arg His Asp Glu Lys Ala Gly Asp 355 360 365 gca gtc gaa aac ttt ttt aac aac caa acg caa aac gcc cgc atc gag 1152 Ala Val Glu Asn Phe Phe Asn Asn Glň Thr Gin Asn Ala Arg Ue Glu 370 375 380 ttg cgc cac caa ccc ata ggt cgt ctg aaa ggc agc tgg ggc gtg caa 1200 Leu Arg His Gin Pro Ile Gly Arg Leu Lys Gly Ser Trp Gly Val Gin 385 390 395 400 tat tta caa caa aaa tcc agt gct tta tet gcc ata tcc gaa gcg gtt 1248 Tyr Leu Gin Gin Lys Ser Ser Ala Leu Ser Ala Ue Ser Glu Ala Val 405 410 415 aaa caa ccg atg ctg ctt gac aac aaa gtg caa cat tac agc ttt ttc 1296 Lys Gin Pro Met Leu Leu Asp Asn Lys Val Gin His Tyr Ser Phe Phe 420 425 430 ggt gta gaa cag gca aac tgg gac aac ttc acg ctt gaa gga ggc gta 1344 Gly Val Glu Gin Ala Asn Trp Asp Asn Phe Thr Leu Glu Gly Gly Val 435 440 445 cgc gtg gaa aaa caa aaa gcc tcc att cag tac gac aaa gca ttg att 1392 Arg Val Glu Lys Gin Lys Ala Ser Ue Gin Tyr Asp Lys Ala Leu Ue 450 - .455 460 gat cgg gaa aac tac tac aac cac ccc ctg ccc gac ctc ggc gcg cac 1440 Asp Arg Glu Asn Tyr Tyr Asn His Pro Leu Pro Asp Leu Gly Ala His 465 470 475 480 cgc caa acc gcc cgc tca ttc gca ctt tcg ggc aac tgg tat ttc acg 1488 Arg Gin Thr Ala Arg Ser Phe Ala Leu Ser Gly Asn Trp Tyr Phe Thr 485 490 495

396 ·'· 99 ♦ <' « · · · · ·'· · 9 '9

9 9 « -9 * » « » ’*

99 99 9 99 9 *· ··-» ·» ·.·

WO 01/85772 PCT/GB01/02003

cca pro caa cac aaa ctc agc ctg acc gcc Leu Thr Ala 505 tcc Ser cat cag gaa cgc ctg ccg Leu Pro X536 Gin His Lys Leu Ser 500 His Gin Glu Arg 510 tca acg caa gag ctg tac gca cac ggc aaa cac gtc gcc acc aac acc 1584 Ser Thr Gin Glu Leu Tyr Ala His Gly Lys His Val Ala Thr Asn Thr 515 520 525 ttt gaa gtc ggc aac aaa cac ctc aac aaa gag cgt tcc aac aat atc 1632 Phe Glu Val Gly Asn Lys His Leu Asn Lys Glu Arg Ser Asn Asn Ile 530 535 540 gaa ctc gcg ctg ggc tac gaa ggc gac cgc tgg caa tac aat ctg gca 1680 Glu Leu Ala Leu Gly Tyr Glu Gly Asp Arg Trp Gin Tyr Asn Leu Ala 545 550 555 560 ctc tac cgc aac cgc ttc ggt aac tac att tac gcc caa acc tta aac 1728 Leu Tyr Arg Asn Arg Phe Gly Asn Tyr Ile Tyr Ala Gin Thr Leu Asn 565 570 575 gac gga cgc ggc ccc aaa tcc atc gaa gac gac agc gaa atg aag ctc 1776 Asp Gly Arg Gly Pro Lys Ser Ile Glu Asp Asp Ser Glu Met Lys Leu 580 585 590 gtg cgc tac aac caa tcc ggc gcc gac ttc tac ggc gcg gaa ggc gaa 1824 Val Arg Tyr Asn Gin Ser Gly Ala Asp Phe Tyr Gly Ala Glu Gly Glu 595 600 605 atc tac ttc aaa ccg aca ccg cgc tac cgc atc ggc gtt tcc ggc gac 1872 Ile Tyr Phe Lys Pro Thr Pro Arg Tyr Arg Ile Gly Val Ser Gly Asp 610 615 620 tat gta ega ggc cgt ctg aaa aac ctg cct tcc cta ccc ggc aga gaa 1920 Tyr Val Arg Gly Arg Leu Lys Asn Leu Pro Ser Leu Pro Gly Arg Glu 625 630 635 640 gat gcc tac ggc aac cgt cct ttc atc gca cag gac gac caa aat gcc 1968 Asp Ala Tyr Gly Asn Arg Pro Phe Ile Ala Gin Asp Asp Gin Asn Ala 645 650 655 ccc cgt gtt ccg gct gcg cgc ctc ggc ttc cac ctg aaa gcc tcg ctg 2016 Pro Arg Val Pro Ala Ala Arg Leu Gly Phe His Leu Lys Ala Ser Leu 660 665 670 acc gac cgt atc gat gcc aat ttg gac tac tac cgc gtg ttc gcc caa 2064 Thr Asp Arg Ile Asp Ala Asn Leu Asp Tyr Tyr Arg Val Phe Ala Gin 675 680 685

397

WO 01/85772 PCT/GB01/02003

aac aaa ctc gcc cgc tac gaa Glu 695 acg cgc acg ccc gga cac cat atg ctc 2112 Asn Lys 690 Leu Ala Arg Tyr Thr Arg Thr Pro Gly 700 His His Met Leu aac ctc ggc gca aac tac cgc cgc aat acg cgc tat ggc gag tgg aat 2160 Asn Leu Gly Ala Asn Tyr Arg Arg Asn Thr Arg Tyr Gly Glu Trp Asn 705 710 715 720 tgg tac gtc aaa gcc gac aac ctg ctc aac caa tcc gtt tac gcc cac 2208 Trp Tyr Val Lys Ala Asp Asn Leu Leu Asn Gin Ser Val Tyr Ala His 725 730 735 age age ttt ctc tet gat acg ccg caa atg ggc cgc age ttt acc ggc 2256 Ser Ser Phe Leu Ser Asp Thr Pro Gin Met Gly Arg Ser Phe Thr Gly 740 745 750 ggc gtg aac gtg aag ttt taa 2277 Gly Val Asn Val Lys Phe

755 <210> 202 <211> 758 <212> PRT

<213> Neisseria meningitidis <400> 202 Met Ala Gin Thr 1 Thr Leu Lys Pro Ile Val Leu Ser Ile Leu Leu Ile 5 10 15 Asn Thr Pro Leu 20 Leu Ala Gin Ala His Glu Thr Glu Gin Ser Val Asp 25 30 Leu Glu Thr Val 35 Ser Val Val Gly Lys Ser Arg Pro Arg Ala Thr Ser 40 45 Gly Leu Leu His 50 Thr Ser Thr Ala Ser Asp Lys Ile Ile Ser Gly Asp 55 60 Thr Leu Arg Gin 65 Lys Ala Val Asn Leu Gly Asp Ala Leu Asp Gly Val 70 75 80 Pro Gly Xle His Ala Ser Gin Tyr Gly Gly Gly Ala Ser Ala Pro val 85 90 95 Ile Arg Gly Gin 100 Thr Gly Arg Arg Ile Lys Val Leu Asn His His Gly 105 110

398

WO 01/85772

Glu Thr

Asp Thr 130

Leu Leu 145

Gly Lys

Gly Leu

Ile Asn.

Tyr Arg 210

Arg Leu 225

Ser Trp

Arg Asp

His Ala

Gin Leu 290

Pro Gly 305

Thr His

Leu Arg

Val His

PCT/GBO1/02003

Gly Asp 115 Met Ala Asp Phe 120 Ser Pro Asp His Ala 125 Ile Met Val Ala Leu Ser Gin Gin 135 Val Glu Ile Leu Arg 140 Gly Pro Val Thr Tyr Ser Ser Gly 150 Asn Val Ala Gly Leu 155 Val Asp Val Ala Asp 160 Ile Pro Glu 165 Lys Met Pro Glu Asn 170 Gly Val Ser Gly Glu 175 Leu Arg Leu 180 Ser Ser Gly Asn Leu 185 Glu Lys Leu Thr Ser 190 Gly Gly Ile 195 Gly Leu Gly Lys Asn 200 Phe Val Leu His Thr 205 Glu Gly Leu Lys Ser Gly Asp Tyr 215 Ala Val Pro Arg Tyr 220 Arg Asn Leu Lys Pco Asp Ser His 230 Ala Asp Ser Gin Thr 235 Gly Ser Ile Gly Leu 240 Val Gly Glu 245 Lys Gly Phe Ile Gly 250 Val Ala Tyr Ser Asp 255 Arg Gin Tyr 260 Gly Leu Pro Ala His 265 Ser His Glu Tyr Asp 270 Asp Cys Asp 275 Ile Ile Trp Gin Lys 280 Ser Leu Ile Asn Lys 285 Arg Tyr Leu Tyr Pro His Leu Leu 295 Thr Glu Glu Asp Ile 300 Asp Tyr Asp Asn Leu Ser Cys Gly 310 Phe His Asp Asp Asp 315 Asn Ala His Ala His 320 Ser Gly Arg 325 Pro' Trp Ile Asp Leu 330 Arg Asn Lys Arg Tyr 335 Glu Ala Glu 340 Trp Lys Gin Pro Phe 345 Pro Gly Phe GlU Ala 350 Leu Arg Leu Asn Arg Asn Asp Tyr Arg His Asp Glu Lys Ala Gly Asp

355 360 365

399

0 ,0 0 · · 00« ··*«««· ·0 ·*0 00 «0

WO 01/85772 PCT/GB01/02003 Ala Val Glu Asn Phe Phe Asn Asn Gin Thr Gin 370 375 Asn Ala Arg Ile Glu 380 Leu Arg His Gin Pro Ile Gly Arg Leu Lys Gly 385 390 395 Ser Trp Gly Val Gin 400 Tyr Leu Gin Gin Lys Ser Ser Ala Leu Ser Ala 405 410 Ile Ser Glu Ala Val 415 Lys Gin Pro Met Leu Leu Asp Asn Lys Val Gin 420 425 His Tyr Ser Phe Phe 430 Gly Val Glu Gin Ala Asn Trp Asp Asn Phe Thr 435 440 Leu Glu Gly Gly Val 445 Arg Val Glu Lys Gin Lys Ala Šer Ile Gin Tyr 450 455 Asp Lys Ala Leu Ile 460 — Asp Arg Glu Asn Tyr Tyr Asn His Pro Leu Pro 465 470 475 Asp Leu Gly Ala His 480 Arg Gin Thr Ala Arg Ser Phe Ala Leu Ser Gly 485 490 Asn Trp Tyr Phe Thr 495 Pro Gin His Lys Leu Ser Leu Thr Ala Ser His 500 505 Gin Glu Arg Leu Pro 510 Ser Thr Gin Glu Leu Tyr Ala His Gly Lys His 515 520 Val Ala Thr Asn Thr 525 Phe Glu Val Gly Asn Lys His Leu Asn Lys Glu 530 535 Arg Ser Asn Asn Ile 540 Glu Leu Ala Leu Gly Tyr Glu Gly Asp Arg Trp 545 550 555 Gin Tyr Asn Leu Ala 560 Leu Tyr Arg Asn Arg Phe Gly Asn Tyr ile Tyr 565 570 Ala Gin Thr Leu Asn 575 Asp Gly Arg Gly Pro Lys' Ser Ile Glu Asp Asp 580 585 Ser Glu Met Lys Leu 590 Val Arg Tyr Asn Gin Ser Gly Ala Asp Phe Tyr 595 600 Gly Ala Glu Gly Glu 605 Ile Tyr Phe Lys Pro Thr Pro Arg Tyr Arg Ile Gly Val Ser Gly Asp

610 615 620

400 • · <· to to ’ to to to · e β to

WO 01/85772 ···· ···· • · « to toto « • to toto • to

PCT/GB01/02003

Tyr Val Arg Gly Arg Leu Lys Asn Leu Pro Ser Leu Pro Gly Arg Glu 625 630 635 640 Asp Ala Tyr Gly Asn Arg Pro Phe Ile Ala Gin Asp Asp Gin Asn Ala 645 650 655 Pro Arg Val Pro Ala Ala Arg Leu Gly Phe His Leu Lys Ala Ser Leu 660 665 670 Thr Asp Arg Ile Asp Ala Asn Leu Asp Tyr Tyr Arg Val Phe Ala Gin 675 680 685 Asn Lys Leu Ala Arg Tyr Glu Thr Arg Thr Pro Gly His His Met Leu 690 695 700 Asn Leu Gly Ala Asn Tyr Arg Arg Asn Thr Arg Tyr Gly Glu Trp Asn 705 710 715 720 Trp Tyr Val Lys Ala Asp Asn Leu Leu Asn Gin Ser Val Tyr Ala His 725 730 735 Ser Ser Phe Leu Ser Asp Thr Pro Gin Met Gly Arg Ser Phe Thr Gly 740 745 750 Gly Val Asn Val Lys Phe

755 <210> 203 <211> 225 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(225) <400> 203

atg agg ctg gca acc aag gat ttg gcg gaa gcc att agg aaa gga cag 48 Met Arg Leu Ala Thr Lys Asp Leu Ala Glu Ala Ile Arg Lys Gly Gin 1 5 10 15 gtt cgc aaa tea agc ttt aac aca gaa caa tta agg gca att gaa aaa 96 Val Arg Lys Ser Ser Phe Asn Thr Glu Gin Leu Arg Ala Ile Glu Lys 20 25 30

401 • · · · ·

WO 01/85772

PCT/GB01/02003

gga gaa tet aaa ata ccg gat tac Ser Lys Ile Pro Asp Tyr Gly Glu 35 40 gga agg atg caa ttg att cgt gaa Gly Arg Met Gin Leu Ile Arg Glu 50 55 att ggt tgg gaa gca atg aac aaa Ile Gly Trp Glu Ala Met Asn Lys 65 70

act tgg cat cat cat caa gat aca 144

Thr Trp His His His Gin Asp Thr ggc ttg cat cat gat acc ggc cat 192 Gly Leu His His Asp Thr Gly His gga agg taa 225

Gly Arg <210> 204 <211> 74 <212> PRT <213> Neisseria meningitidis <400> 204

Met Arg Leu Ala Thr Lys Asp Leu Ala Glu Ala Ile Arg Lys Gly Gin 1 5 10 15 Val Arg Lys Ser Ser Phe Asn Thr Glu Gin Leu Arg Ala Ile Glu Lys 20 25 30 Gly Glu Ser Lys Ile Pro Asp Tyr Thr Trp His His His Gin Asp Thr 35 40 45 Gly Arg Met Gin Leu Ile Arg Glu Gly Leu His His Asp Thr Gly His 50 55 60 Ile Gly Trp Glu Ala Met Asn Lys Gly Arg

65 70

<210> 205 <211> 1176 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)··(1176) <400> 205 atg gca aaa atg atg aaa tgg gcg gct gtt gcg gcg gtc gcg gcg gca 48 Met Ala Lys Met Met Lys Trp Ala Ala Val Ala Ala Val Ala Ala Ala

402 · fcfc fcfc fcfc * ·· ···· fc · · · fcfc fcfc fcfc fc • fcfcfcfc · fc · • · · fc · fc · fc fc · • fc β e fc fc e · fc ···· ···· ·· ··· «fc ··

WO 01/85772 PCT/GB01/02003

1 5 10 15

gcg Ala gtt tgg ggc gga tgg tet Ser tat ctg aag ccc gag ccg cag gct gct 96 Val Trp Gly Gly Trp 20 Tyr Leu 25 Lys Pro Glu Pro Gin 30 Ala Ala tat att acg gaa acg gtc agg cgc ggc gac atc agc cgg acg gtt tet 144 Tyr Ile Thr Glu Thr Val Arg Arg Gly Asp Ile Ser Arg Thr Val Ser 35 40 45 gca aca ggg gag att tcg ccg tcc aac ctg gta tcg gtc ggc gcg cag 192 Ala Thr Gly GlU Ile Ser Pro Ser Asn Leu Val Ser Val Gly Ala Gin 50 55 60 gca tcg ggg cag att aag aaa ctt tat gtc aaa ctc ggg caa cag gtt 240 Ala Ser Gly Gin Ile Lys Lys Leu Tyr Val Lys Leu Gly Gin Gin Val 65 70 75 80 aaa aag ggc gat ttg att gcg gaa atc aat tcg acc tcg cag acc aat 288 Lys Lys Gly Asp Leu Ile Ala Glu Ile Asn Ser Thr Ser Gin Thr Asn 85 90 95 acg ctc aat acg gaa aaa tcc aaa ttg gaa acg tat cag gcg aag ctg 336 Thr Leu Asn Thr Glu Lys Ser Lys Leu Glu Thr Tyr Gin Ala Lys Leu 100 105 110 gtg tcg gca cag att gca ttg ggc agc gcg gag aag aaa tat aag cgt 384 Val Ser Ala Gin Ile Ala Leu Gly Ser Ala Glu Lys Lys Tyr Lys Arg 115 120 125 cag gcg gcg ttg tgg aag gat gat gcg acc gct aaa gaa gat ttg gaa 432 Gin Ala Ala Leu Trp Lys Asp Asp Ala Thr Ala Lys Glu Asp Leu Glu 130 135 140 agc gca cag gat gcg ctt gcc gcc gcc aaa gcc aat gtt gcc gag ctg 480 Ser Ala Gin Asp Ala Leu Ala Ala Ala Lys Ala Asn Val Ala Glu Leu 145 150 155 160 aag gct cta atc aga cag agc aaa att tcc atc aat acc gcc gag tcg 528 Lys Ala Leu Ile Arg Gin Ser Lys Ile Ser Ile Asn Thr Ala Glu Ser 165 • 170 175 gaa ttg ggc tac acg cgc att acc gca acg atg gac ggc acg gtg gtg 576 Glu Leu Gly Tyr Thr Arg Ile Thr Ala Thr Met Asp Gly Thr Val val 180 185 190 gcg att ctc gtg gaa gag ggg cag act gtg aac gcg gcg cag tet acg 624 Ala Ile Leu Val Glu Glu Gly Gin Thr Val Asn Ala Ala Gin Ser Thr

403

ΦΦ «ο ·· ·· φ φφ ··· • •φφ φφφφ φφ φ φ φ φ φ φ φ φ φ φφφφ φφφφ φ φ φ φ β # φφφφ φφφφ φφφφ φφ φφφ φφ φφ

W0 01/85772 PCT/GB01/02003

¹⁹⁵ 200 205 ccg acg att gtc caa ttg gcg aat ctg gat atg atg ttg aac aaa atg 672 Pro Thr Ile Val Gin Leu Ala Asn Leu Asp Met Met Leu Asn Lys Met 210 215 220 cag att gcc gag ggc gat att acc aag gtg aag gcg ggg cag gat att 720 Gin Ile Ala Glu Gly Asp Ile Thr Lys Val Lys Ala Gly Gin Asp Ile 225 230 235 240 tcg ttt acg att ttg tcc gaa ccg gat acg ccg att aag gcg aag ctc 768 Ser Phe Thr Ile Leu Ser Glu Pro Asp Thr Pro Ile Lys Ala Lys Leu 245 250 255 gac age gtc gac CCC ggg ctg acc acg atg tcg tcg ggc ggc tac aac 816 Asp Ser Val Asp Pro Gly Leu Thr Thr Met Ser Ser Gly Gly Tyr Asn 260 265 270 age agt acg gat acg get tcc aat gcg gtc tac tat tat gcc cgt tcg 864 Ser Ser Thr Asp Thr Ala Ser Asn Ala Val Tyr Tyr Tyr Ala Arg Ser 275 280 285 ttt gtg ccg aat ccg gac ggc aaa ctc gcc acg ggg atg acg acg cag 912 Phe Val Pro Asn Pro Asp Gly Lys Leu Ala Thr Gly Met Thr Thr Gin 290 295 300 aat acg gtt gaa atc gac ggt gtg aaa aat gtg ctg att att ccg tcg 960 Asn Thr Val Glu Ile Asp Gly Val Lys Asn Val Leu Ile Ile Pro Ser 305 310 315 320 ctg acc gtg aaa aat cgc ggc ggc agg gcg ttt gtg cgc gtg ttg ggt 1008 Leu Thr Val Lys Asn Arg Gly Gly Arg Ala Phe Val Arg Val Leu Gly 325 330 335 gca gac ggc aag gcg gcg gaa cgc gaa atc cgg acc ggt atg aga gac 1056 Ala Asp Gly Lys Ala Ala Glu Arg Glu Ile Arg Thr Gly Met Arg Asp 340 345 350 agt atg aat acc gaa gta aaa age ggg ttg aaa gag ggg gac aaa gtg 1104 Ser Met Asn Thr Glu Val ,Lys ser Gly Leu Lys Glu Gly Asp Lys Val 355 360 365 gtc atc tcc gaa ata acc gcc gcc gag cag cag gaa age ggc gaa cgc 1152 Val Ile Ser Glu Ile Thr Ala Ala Glu Gin Gin Glu Ser Gly Glu Arg 370 375 380 gcc cta ggc ggc ccg ccg cgc ega 1176 Ala Leu Gly Gly Pro Pro Arg Arg

404

• »* • · · ·· · • 4 ·· ·« ···· • · · Ο « e e ·· ··· β β « β • · · ·

WO 01/85772 PCT/GBO1/02003 385 390 <210> 206 <211> 392 <212> PRT <213> Neisseria meningitidis <400> 206 Met Ala Lys 1 Met Met Lys Trp Ala Ala Val Ala Ala Val 5 10 Ala Ala Ala 15 Ala Val Trp Gly Gly Trp Ser Tyr Leu Lys Pro Glu Pro 20 25 Gin Ala Ala 30 Tyr Ile Thr 35 Glu Thr Val Arg Arg Gly Asp Ile Ser Arg 40 45 Thr Val Ser Ala Thr Gly 50 Glu Ile Ser Pro Ser Asn Leu Val Ser Val 55 60 Gly Ala Gin Ala Ser Gly 65 Gin Ile Lys Lys Leu Tyr Val Lys Leu Gly 70 75 Gin Gin Val 80 Lys Lýs Gly Asp Leu Ile Ala Glu Ile Asn Ser Thr Ser 85 90 Gin Thr Asn 95 Thr Leu Asn Thr Glu Lys Ser Lys Leu Glu Thr Tyr Gin 100 105 Ala Lys Leu HO Val Ser Ala 115 Gin Ile Ala Leu Gly Ser Ala Glu Lys Lys 120 125 Tyr Lys Arg Gin Ala Ala 130 Leu Trp Lys Asp Asp Ala Thr Ala Lys Glu 135 140 Asp Leu Glu Ser Ala Gin 145 Asp Ala Leu Ala Ala Ala Lys Ala Asn Val 150 155 Ala Glu Leu 160 Lys Ala Leu Ile Arg Gin Ser Lys Ile Ser Ile Asn Thr 165 * 170 Ala Glu Ser 175 Glu Leu Gly Tyr Thr Arg Ile Thr Ala Thr Met Asp Gly 180 185 Thr Val Val 190 Ala Ile Leu 195 Val Glu Glu Gly Gin Thr Val Asn Ala Ala 200 205 Gin Ser Thr

405

WO 01/85772 PCT/GB01/02003 Pro Thr Ile Val Gin Leu Ala Asn Leu Asp Met 210 215 Met Leu Asn Lys Met 220 Gin Ile Ala Glu Gly Asp Ile Thr Lys Val Lys 225 230 235 Ala Gly Gin Asp Ile 240 Ser Phe Thr Ile Leu Ser Glu Pro Asp Thr Pro 245 250 Ile Lys Ala Lys Leu 255 Asp Ser Val Asp Pro Gly Leu Thr Thr Met Ser 260 265 Ser Gly Gly Tyr Asn 270 Ser Ser Thr Asp Thr Ala Ser Asn Ala Val Tyr 275 280 Tyr Tyr Ala Arg Ser 285 Phe Val Pro Asn Pro Asp Gly Lys Leu Ala Thr 290 295 Gly Met Thr Thr Gin 300 Asn Thr Val Glu Ile Asp Gly Val Lys Asn Val 305 310 315 Leu Ile Ile Pro Ser 320 Leu Thr Val Lys Asn Arg Gly Gly Arg Ala Phe 325 330 Val Arg Val Leu Gly 335 Ala Asp Gly Lys Ala Ala Glu Arg Glu Ile Arg 340 345 Thr Gly Met Arg Asp 350 Ser Met Asn .Thr Glu Val Lys Ser Gly Leu Lys 355 360 Glu Gly Asp Lys Val 365 Val Ile Ser Glu Ile Thr Ala Ala Glu Gin Gin 370 375 Ala Leu Gly Gly Pro Pro Arg Arg Glu Ser Gly Glu Arg 380

385 390 <210> 207 <211> 342 <212> DNA <213> Neisseria meningitidis <220>

<221> CDS <222> (1)..(342) <400> 207

406 ·· ·· · • · · · « « · « · 4 * · · · · « · · • « · ♦ · · · · · · e # e ě i e & « « ···· ···· ·· ·*· ·· «·

WO 01/85772 PCT/GBO1/02003 ·· ·«··

ttg ttt gta tgc ttt Phe 5 gaa Glu aaa Lys tgt Cys ttg ttt ccg gat ttt gcc Phe Ala att Ile 15 ccc Pro 48 Leu 1 Phe Val Cys Leu Phe 10 Pro Asp atc cgg ttt tgc get gta ega tgt gtt tta gcg cgg act tgc tea aaa 96 Ile Arg Phe Cys Ala Val Arg Cys Val Leu Ala Arg Thr Cys Ser Lys 20 25 30 tcg gat gtg gtt ccg gta ttt ggg get ttg att agg gat gcg gac ttt 144 Ser Asp Val Val Pro Val Phe Gly Ala Leu Ile Arg Asp Ala Asp Phe 35 40 45 caa tat att ttt ctc age tac aac aac gaa ggc ttg atg tet gtc ggg 192 Gin Tyr Ile Phe Leu Ser Tyr Asn Asn Glu Gly Leu Met Ser Val Gly 50 55 60 cag gta agg gag att ttt gag cgt ttc ggc aaa tat aat ttg gtt caa 240 Gin Val Arg Glu Ile Phe Glu Arg Phe Gly Lys Tyr Asn Leu Val Gin 65 70 75 80 acg gaa tat cgg cgt ttt aag gca gat aag aca gaa aac cgt aat cat 288 Thr Glu Tyr Arg Arg Phe Lys Ala Asp Lys Thr Glu Asn Arg Asn His 85 90 95 aag gca aat tcg ata ttc gaa tat ctg cat gag acc ttt gca aaa ata 336 Lys Ala Asn Ser Ile Phe Glu Tyr Leu His Glu Thr Phe Ala Lys Ile 100 105 110

gtc tgt .342

Val Cys <210> 208 <211> 114 <212> PRT <213> Neisseria meningitidis <400> 208

Leu 1 Phe Val Cys Phe 5 Glu Lys Cys Leu Phe 10 Pro Asp Phe Ala Ile 15 Pro Ile Arg Phe Cys 20 Ala Val Arg Cys val 25 Leu Ala Arg Thr Cys 30 Ser Lys Ser Asp Val 35 Val Pro Val Phe Gly 40 Ala Leu Ile Arg Asp 45 Ala Asp Phe Gin Tyr Ile Phe Leu Ser Tyr Asn Asn Glu Gly Leu Met Ser val Gly

407 /

·· ·· • 4 4 · • · • · e β

44444444

44 4 44 4444

4 9 4 4 4 4 4

9 4 4 4 4 4

4 4 4 9 4 4 4

4 4 4 4 4 4 ®

94 944 · ··

WO 01/85772

50 55

Gin Val Arg Glu Ile Phe Glu Arg Phe 65 70

Thr Glu Tyr Arg Arg Phe Lys Ala Asp 85

Lys Ala Asn Ser Ile Phe Glu Tyr Leu 100 105

Val Cys

PCT/GBO1/02003

60 Gly Lys 75 Tyr Asn Leu Val Gin 80 Lys 90 Thr Glu Asn Arg Asn 95 His His Glu Thr Phe Ala Lys Ile

110 <210> 209 <211> 24 <212> DNA <2i3> syntetická sekvence <220>

<223> Popis syntetické sekvence: sekvence primeru <400> 209 ttggttaatt ggttgtaaca ctgg 24 <210> 210 <211> 19 <212> DNA <2i3> syntetická sekvence <220>

<223> Popis syntetické sekvence: sekvence primeru <400> 210 attctcatgt ttgacagcg 19 <210> 211 <211> 18 <212> DNA <2i3> syntetická sekvence <220>

<223> Popis syntetické sekvence: sekvence primeru

408 ě

···

WO 01/85772

PCT/GBO1/02003 ·« ·· • · · · β e ···· ···· ·· ···· • · « • · · • · · e e · · ·· ·· <400> 211 atcctacaac ctcaagct 18 <210> 212 <211> 18 <212> DNA <213> syntetická sekvence <22 0>

<223> Popis syntetické sekvence: sekvence primeru ;

<400> 212 atcccattct aaccaagc 18 <210> 213 <211> 19 <212> DNA <2i3> syntetická sekvence <220>

<223> Popis syntetické sekvence: sekvence primeru <400> 213 aagagattac gcgcagacc 19 <210> 214 <211> 18 <212> DNA <2i3> syntetická sekvence <220>

<223> Popis syntetické sekvence: sekvence primeru <400> 214 aatacgcaac cgcctctc 18