DD141952A5 - Verfahren zur bestimmung der biologischen aktivitaet von heparin - Google Patents

Verfahren zur bestimmung der biologischen aktivitaet von heparin Download PDF

Info

Publication number: DD141952A5
Authority: DD; German Democratic Republic
Prior art keywords: heparin; thrombin; iii; chromogenic substrate; arg
Prior art date: 1978-03-23

Application number

DD79210876A

Other languages

German (de)

English (en)

Inventor

Knut Bartl

Helmut Lill

Joachim Ziegenhorn

Original Assignee

Boehringer Mannheim Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1978-03-23

Filing date

1979-02-07

Publication date

1980-05-28

1979-02-07 Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh

1980-05-28 Publication of DD141952A5 publication Critical patent/DD141952A5/de

Links

HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 title claims description 32
229960002897 heparin Drugs 0.000 title claims description 32
229920000669 heparin Polymers 0.000 title claims description 32
238000000034 method Methods 0.000 title claims description 27
230000004071 biological effect Effects 0.000 title claims description 6
108090000190 Thrombin Proteins 0.000 claims description 20
229960004072 thrombin Drugs 0.000 claims description 18
239000003593 chromogenic compound Substances 0.000 claims description 13
239000000872 buffer Substances 0.000 claims description 7
108010074860 Factor Xa Proteins 0.000 claims description 6
108091005804 Peptidases Proteins 0.000 claims description 6
239000003153 chemical reaction reagent Substances 0.000 claims description 6
108090000935 Antithrombin III Proteins 0.000 claims description 5
102000035195 Peptidases Human genes 0.000 claims description 5
102000004411 Antithrombin III Human genes 0.000 claims description 4
108010039627 Aprotinin Proteins 0.000 claims description 4
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 4
229960005348 antithrombin iii Drugs 0.000 claims description 4
229960004405 aprotinin Drugs 0.000 claims description 4
ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 claims description 4
239000000758 substrate Substances 0.000 claims description 4
239000002202 Polyethylene glycol Substances 0.000 claims description 3
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 3
229920001223 polyethylene glycol Polymers 0.000 claims description 3
108090000765 processed proteins & peptides Proteins 0.000 claims description 3
YDMBNDUHUNWWRP-VJBWXMMDSA-N (2s)-1-[(2r)-2-amino-3-phenylpropanoyl]-n-[(2s)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]piperidine-2-carboxamide Chemical compound C([C@@H](N)C(=O)N1[C@@H](CCCC1)C(=O)N[C@@H](CCCN=C(N)N)C(=O)NC=1C=CC(=CC=1)[N+]([O-])=O)C1=CC=CC=C1 YDMBNDUHUNWWRP-VJBWXMMDSA-N 0.000 claims description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 2
108010039286 S 2238 Proteins 0.000 claims description 2
239000003513 alkali Substances 0.000 claims description 2
210000002381 plasma Anatomy 0.000 description 14
230000000694 effects Effects 0.000 description 8
239000000975 dye Substances 0.000 description 7
108090000790 Enzymes Proteins 0.000 description 6
102000004190 Enzymes Human genes 0.000 description 6
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
230000015271 coagulation Effects 0.000 description 6
238000005345 coagulation Methods 0.000 description 6
229940088598 enzyme Drugs 0.000 description 6
RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 6
238000006243 chemical reaction Methods 0.000 description 5
208000007536 Thrombosis Diseases 0.000 description 4
210000004369 blood Anatomy 0.000 description 4
239000008280 blood Substances 0.000 description 4
230000035945 sensitivity Effects 0.000 description 4
238000002560 therapeutic procedure Methods 0.000 description 4
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
230000002779 inactivation Effects 0.000 description 3
238000011534 incubation Methods 0.000 description 3
230000008569 process Effects 0.000 description 3
239000011780 sodium chloride Substances 0.000 description 3
YSRMCZDHEZNRPS-HOTGVXAUSA-N (2s)-5-(diaminomethylideneamino)-2-[[(2s)-1-[2-[(4-methylphenyl)sulfonylamino]acetyl]pyrrolidine-2-carbonyl]amino]pentanoic acid Chemical compound C1=CC(C)=CC=C1S(=O)(=O)NCC(=O)N1[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)CCC1 YSRMCZDHEZNRPS-HOTGVXAUSA-N 0.000 description 2
VYLJFJGMPYBPMN-VXKWHMMOSA-N (2s)-n-[(2s)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]-1-[2-[(4-methylphenyl)sulfonylamino]acetyl]pyrrolidine-2-carboxamide Chemical compound C1=CC(C)=CC=C1S(=O)(=O)NCC(=O)N1[C@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)NC=2C=CC(=CC=2)[N+]([O-])=O)CCC1 VYLJFJGMPYBPMN-VXKWHMMOSA-N 0.000 description 2
ZYECEUZMVSGTMR-LBDWYMBGSA-N (4s)-4-[[(2s,3s)-2-benzamido-3-methylpentanoyl]amino]-5-[[2-[[(2s)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]amino]-2-oxoethyl]amino]-5-oxopentanoic acid Chemical compound N([C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CCCN=C(N)N)C(=O)NC=1C=CC(=CC=1)[N+]([O-])=O)C(=O)C1=CC=CC=C1 ZYECEUZMVSGTMR-LBDWYMBGSA-N 0.000 description 2
102000009123 Fibrin Human genes 0.000 description 2
108010073385 Fibrin Proteins 0.000 description 2
BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 2
239000007983 Tris buffer Substances 0.000 description 2
108010031969 benzoyl-Ile-Glu-Gly-Arg-p-nitroanilide Proteins 0.000 description 2
108010018472 chromozym TH Proteins 0.000 description 2
229950003499 fibrin Drugs 0.000 description 2
238000005259 measurement Methods 0.000 description 2
239000000243 solution Substances 0.000 description 2
238000010998 test method Methods 0.000 description 2
TYMLOMAKGOJONV-UHFFFAOYSA-N 4-nitroaniline Chemical compound NC1=CC=C([N+]([O-])=O)C=C1 TYMLOMAKGOJONV-UHFFFAOYSA-N 0.000 description 1
102100022977 Antithrombin-III Human genes 0.000 description 1
206010053567 Coagulopathies Diseases 0.000 description 1
208000005189 Embolism Diseases 0.000 description 1
108010049003 Fibrinogen Proteins 0.000 description 1
102000008946 Fibrinogen Human genes 0.000 description 1
208000032843 Hemorrhage Diseases 0.000 description 1
239000004365 Protease Substances 0.000 description 1
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
239000002253 acid Substances 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
230000004913 activation Effects 0.000 description 1
230000006978 adaptation Effects 0.000 description 1
239000000654 additive Substances 0.000 description 1
230000000996 additive effect Effects 0.000 description 1
229910001514 alkali metal chloride Inorganic materials 0.000 description 1
125000003277 amino group Chemical group 0.000 description 1
125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 1
230000008901 benefit Effects 0.000 description 1
230000007321 biological mechanism Effects 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
239000007853 buffer solution Substances 0.000 description 1
238000011088 calibration curve Methods 0.000 description 1
230000008859 change Effects 0.000 description 1
230000035602 clotting Effects 0.000 description 1
230000002349 favourable effect Effects 0.000 description 1
229940012952 fibrinogen Drugs 0.000 description 1
239000007850 fluorescent dye Substances 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
QQVNCBCBFNWLJX-KCHLEUMXSA-N n-[(2s)-1-[[(2s)-1-[[(2s)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-1-oxo-3-phenylpropan-2-yl]benzamide Chemical compound C([C@@H](C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCN=C(N)N)C(=O)NC=1C=CC(=CC=1)[N+]([O-])=O)NC(=O)C=1C=CC=CC=1)C1=CC=CC=C1 QQVNCBCBFNWLJX-KCHLEUMXSA-N 0.000 description 1
102000004196 processed proteins & peptides Human genes 0.000 description 1
230000002797 proteolythic effect Effects 0.000 description 1
238000004445 quantitative analysis Methods 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
238000001228 spectrum Methods 0.000 description 1
238000006467 substitution reaction Methods 0.000 description 1
238000001356 surgical procedure Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/10—Anilides
- C12Q2337/12—Para-Nitroanilides p-NA
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/81—Protease inhibitors
- G01N2333/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- G01N2333/811—Serine protease (E.C. 3.4.21) inhibitors
- G01N2333/8114—Kunitz type inhibitors
- G01N2333/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
- G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
- G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
- G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
- G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
- G01N2333/96433—Serine endopeptidases (3.4.21)
- G01N2333/96441—Serine endopeptidases (3.4.21) with definite EC number
- G01N2333/96444—Factor X (3.4.21.6)
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/974—Thrombin
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
- G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- G01N2400/38—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence, e.g. gluco- or galactomannans, Konjac gum, Locust bean gum or Guar gum
- G01N2400/40—Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/108331—Preservative, buffer, anticoagulant or diluent
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Neurosurgery (AREA)
Biotechnology (AREA)
Immunology (AREA)
Analytical Chemistry (AREA)
Molecular Biology (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Hematology (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)

DD79210876A 1978-03-23 1979-02-07 Verfahren zur bestimmung der biologischen aktivitaet von heparin DD141952A5 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE2812943A DE2812943C3 (de)	1978-03-23	1978-03-23	Verfahren und Reagens zur Bestimmung der biologischen Aktivität von Heparin im Plasma

Publications (1)

Publication Number	Publication Date
DD141952A5 true DD141952A5 (de)	1980-05-28

Family

ID=6035376

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DD79210876A DD141952A5 (de)	1978-03-23	1979-02-07	Verfahren zur bestimmung der biologischen aktivitaet von heparin

Country Status (22)

Country	Link
US (1)	US4234682A (da)
EP (1)	EP0004271A3 (da)
JP (1)	JPS54128796A (da)
AR (1)	AR217503A1 (da)
AT (1)	AT362080B (da)
AU (1)	AU512206B2 (da)
BE (1)	BE875014A (da)
CA (1)	CA1111334A (da)
CH (1)	CH642749A5 (da)
DD (1)	DD141952A5 (da)
DE (1)	DE2812943C3 (da)
DK (1)	DK149475C (da)
FI (1)	FI62859C (da)
FR (1)	FR2420764A1 (da)
GB (1)	GB2017298B (da)
HU (1)	HU176068B (da)
IE (1)	IE48067B1 (da)
IT (1)	IT1112966B (da)
LU (1)	LU81070A1 (da)
NL (1)	NL7902259A (da)
SE (1)	SE445560B (da)
ZA (1)	ZA79622B (da)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE2903701C2 (de) *	1979-01-31	1980-10-16	Boehringer Mannheim Gmbh, 6800 Mannheim	Kontrollreagens für die Bestimmung der Heparinaktivität
US4244865A (en) *	1979-12-03	1981-01-13	Abbott Laboratories	α hydroxy tripeptide substrates
DE3005540A1 (de) *	1980-02-14	1981-08-20	Boehringer Mannheim Gmbh, 6800 Mannheim	Verfahren und reagens zur bestimmung der biologischen aktivitaet von heparin im plasma
DE3019612A1 (de) *	1980-05-22	1981-11-26	Boehringer Mannheim Gmbh, 6800 Mannheim	Stabilisiertes thrombinpraeparat
JPH0258920B2 (da) *	1980-10-09	1990-12-11	Boehringer Mannheim Gmbh
US4473639A (en) *	1982-09-15	1984-09-25	Miles Laboratories, Inc.	Reagent strip test for antithrombin-III
US4543335A (en) *	1982-12-20	1985-09-24	Miles Laboratories, Inc.	Device and method for the quantitative determination of heparin in mammalian blood plasma
US4851336A (en) *	1985-09-05	1989-07-25	Yin E Thye	Composition, kit, and method for assaying heparinano a method for making the composition
US4948724A (en) *	1985-09-05	1990-08-14	Yin E Thye	Composition, kit and method for assaying heparin and a method for making the composition
US4946775A (en) *	1985-09-05	1990-08-07	Yin E Thye	Composition, kit and method for assaying heparin and a method for making the composition
DE3679763D1 (de) *	1985-09-05	1991-07-18	E Thye Yin	Verfahren und zusammensetzungen zur bestimmung von heparin.
US5939325A (en) *	1989-07-20	1999-08-17	Analytical Control Systems, Inc.	Stable whole blood coagulation controls
EP0482088B1 (en) *	1989-07-20	1999-03-31	Analytical Control Systems, Inc.	Improved stable coagulation controls
JP3094165B2 (ja) *	1990-11-05	2000-10-03	バクスター、ダイアグノスチックス、インコーポレイテッド	抗凝固剤濃度測定方法
US5308755A (en) *	1992-06-08	1994-05-03	Research Corporation Technologies, Inc.	Method for measuring heparin
EP1325962A1 (en) *	2001-12-17	2003-07-09	Gentium S.p.A.	A method for determining the biological activity of defibrotide
AU2005243161A1 (en) *	2004-05-11	2005-11-24	Heptest Laboratories, Inc.	Compositions, kit and one-step method for monitoring compounds having anti-factor Xa and/or anti factor iia activities
US7219827B2 (en)	2004-11-19	2007-05-22	General Electric Company	Braze end isolation layer for generator armature winding bar and method for applying the isolation layer
DE102005038418A1 (de) *	2005-08-12	2007-02-15	Dade Behring Marburg Gmbh	Faktor Xa-basierter Heparinassay unter Verwendung einer Heparin-modifizierenden Komponente
DK2637672T3 (da)	2010-11-12	2018-10-22	Gentium S R L	Defibrotid til anvendelse i profylakse og/eller behandling af graft versus host sygdom (gvhd)
EP2484775A1 (de)	2011-02-07	2012-08-08	Siemens Healthcare Diagnostics Products GmbH	Heparin-insensitives Verfahren zur Bestimmung von direkten Gerinnungsfaktorinhibitoren
KR102038357B1 (ko)	2012-06-22	2019-10-31	젠티엄 에스알엘	디파이브로타이드의 생물학적 활성 측정을 위한 유우글로불린에 기초한 방법
EP2843416A1 (de)	2013-09-02	2015-03-04	Siemens Healthcare Diagnostics Products GmbH	Kontroll- und Kalibratormaterial für die Bestimmung von direkten Antikoagulanzien
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EP3348649A1 (de)	2017-01-16	2018-07-18	Siemens Healthcare Diagnostics Products GmbH	Universales kontrollmaterial für gerinnungsfaktorinhibitor-teste
EP4703478A1 (de)	2024-08-27	2026-03-04	Siemens Healthcare Diagnostics Products GmbH	Verfahren zum ausschluss von faktor xia-inhibitoren in patientenproben

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1978
- 1978-03-23 DE DE2812943A patent/DE2812943C3/de not_active Expired
1979
- 1979-01-16 EP EP79100122A patent/EP0004271A3/de not_active Withdrawn
- 1979-01-16 AT AT32179A patent/AT362080B/de not_active IP Right Cessation
- 1979-01-31 AU AU43825/79A patent/AU512206B2/en not_active Ceased
- 1979-02-07 JP JP1234279A patent/JPS54128796A/ja active Granted
- 1979-02-07 CA CA321,067A patent/CA1111334A/en not_active Expired
- 1979-02-07 DD DD79210876A patent/DD141952A5/de not_active IP Right Cessation
- 1979-02-08 IE IE238/79A patent/IE48067B1/en not_active IP Right Cessation
- 1979-02-12 DK DK57079A patent/DK149475C/da active
- 1979-02-13 HU HU79BO1763A patent/HU176068B/hu not_active IP Right Cessation
- 1979-02-13 FI FI790476A patent/FI62859C/fi not_active IP Right Cessation
- 1979-02-13 ZA ZA79622A patent/ZA79622B/xx unknown
- 1979-02-14 AR AR275507A patent/AR217503A1/es active
- 1979-02-21 US US06/013,759 patent/US4234682A/en not_active Expired - Lifetime
- 1979-03-20 CH CH261079A patent/CH642749A5/de not_active IP Right Cessation
- 1979-03-21 GB GB7909974A patent/GB2017298B/en not_active Expired
- 1979-03-21 SE SE7902555A patent/SE445560B/sv not_active IP Right Cessation
- 1979-03-22 NL NL7902259A patent/NL7902259A/xx active Search and Examination
- 1979-03-22 IT IT21220/79A patent/IT1112966B/it active
- 1979-03-22 BE BE194147A patent/BE875014A/xx not_active IP Right Cessation
- 1979-03-23 LU LU81070A patent/LU81070A1/de unknown
- 1979-03-23 FR FR7907424A patent/FR2420764A1/fr active Granted

Also Published As

Publication number	Publication date
HU176068B (en)	1980-12-28
AT362080B (de)	1981-04-27
FI62859B (fi)	1982-11-30
FI790476A7 (fi)	1979-09-24
AR217503A1 (es)	1980-03-31
FI62859C (fi)	1983-03-10
DE2812943B2 (de)	1980-08-14
LU81070A1 (de)	1979-06-19
GB2017298A (en)	1979-10-03
AU512206B2 (en)	1980-09-25
DK149475B (da)	1986-06-23
NL7902259A (nl)	1979-09-25
EP0004271A2 (de)	1979-10-03
CH642749A5 (de)	1984-04-30
ZA79622B (en)	1980-03-26
IE790238L (en)	1979-09-23
US4234682A (en)	1980-11-18
SE7902555L (sv)	1979-09-24
EP0004271A3 (de)	1980-04-02
DK57079A (da)	1979-09-24
ATA32179A (de)	1980-09-15
IT7921220A0 (it)	1979-03-22
DE2812943A1 (de)	1979-10-04
IT1112966B (it)	1986-01-20
JPS54128796A (en)	1979-10-05
DE2812943C3 (de)	1981-05-14
FR2420764B1 (da)	1983-07-29
CA1111334A (en)	1981-10-27
AU4382579A (en)	1979-09-27
FR2420764A1 (fr)	1979-10-19
SE445560B (sv)	1986-06-30
BE875014A (fr)	1979-09-24
IE48067B1 (en)	1984-09-19
JPS5726120B2 (da)	1982-06-02
GB2017298B (en)	1982-07-07
DK149475C (da)	1986-12-01

Legal Events

Date	Code	Title	Description
1995-12-21	ENJ	Ceased due to non-payment of renewal fee

Publication	Publication Date	Title
DE2812943C3 (de)	1981-05-14	Verfahren und Reagens zur Bestimmung der biologischen Aktivität von Heparin im Plasma
EP0158254B1 (de)	1988-06-08	Reagenz zur Bestimmung der Thromboplastinzeit
EP0034320B1 (de)	1984-05-16	Verfahren und Reagens zur Bestimmung der biologischen Aktivität von Heparin im Plasma
EP0915340B1 (de)	2006-01-04	Verfahren zur Bestimmung des antikoagulatorischen Potentials einer Probe und Verfahren zur Bestimmung der Glykosilierung von Thrombomodulin
DE60315151T2 (de)	2008-04-10	Diagnostischer test zur bestimmung der konzentration einer transienten proteolytischen aktivität in zusammengesetzten biologischen medien
EP0537490B1 (de)	1997-02-12	Funktioneller Test und Reagenz zur Bestimmung von Fibrinogen
DD222043A5 (de)	1985-05-08	Verfahren und reagens zur gleichzeitigen bestimmung von fibrinogen und fibrinogen-spaltprodukten im plasma
DE608235T1 (de)	1994-12-22	Vefahren zur diagnose von störungen der blutgerinnung.
EP0696642B1 (de)	2002-07-03	Verfahren zum Nachweis von Störungen des Protein C/Protein S-Systems
EP0216179B1 (de)	1991-08-21	Verfahren zur Bestimmung von Proteaseinhibitoren
EP0229234A1 (de)	1987-07-22	Verfahren zur photometrischen Bestimmung von Protein C
DE68919338T2 (de)	1995-05-24	Fibrinolytischer Test.
DE2601372C3 (de)	1980-03-27	Verfahren zur Bestimmung von Antithrombin III
EP0656424A2 (de)	1995-06-07	Test zur Bestimmung der Empfindlichkeit gegenüber aktiviertem Protein C
DE60009879T2 (de)	2005-03-31	Bestimmung von biologisch aktiven formen von proteolytischen enzymen
DE10116586B4 (de)	2005-07-21	Testsystem zur Bestimmung der Hämostaseaktivierung von biologischen Flüssigkeiten
DE69111399T2 (de)	1996-02-29	Chromogenes bestimmungsverfahren für den faktor viii:ca.
DE102012014339A1 (de)	2013-01-24	Verwendung eines Substrats in einem Verfahren zur Messung der Aktivität von vorhandenen proteolytischen Enzymen
EP2486145B1 (de)	2014-07-30	Verfahren zur Bestimmung von Faktor XIII mit Hilfe von Referenzmaterial auf Plasmabasis
DE69916816T2 (de)	2004-09-30	Verbesserter blutgerinnungstest
EP0458329B1 (de)	1996-01-31	Verfahren zum Nachweis von Plasminogen-Aktivatoren, deren inhibitoren oder Stimulatoren
DE3838529A1 (de)	1990-05-17	Globaltest zur erfassung der hauptkomponenten des fibrinolysesystems
WO2006089697A1 (de)	2006-08-31	Verfahren zur bestimmung der gesamtgerinnungsaktivität einer blut- oder plasmaprobe
DD250187A5 (de)	1987-09-30	Verfahren zur photometrischen Bestimmung von Protein C
DD156295A5 (de)	1982-08-11	Verfahren zur bestimmung der biologischen aktivitaet von heparin