DD208988A5 - Verfahren zum aufbau eines chimaeren plasmids mit einer genkodierung fuer eine thermostabile alpha-amylase - Google Patents

Verfahren zum aufbau eines chimaeren plasmids mit einer genkodierung fuer eine thermostabile alpha-amylase Download PDF

Info

Publication number: DD208988A5
Authority: DD; German Democratic Republic
Prior art keywords: amylase; plasmid; alpha; cells; gene
Prior art date: 1981-01-15

Application number

DD82236740A

Other languages

German (de)

English (en)

Inventor

Jonathan R Mielenz

Susan Mickel

Original Assignee

Cpc International Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1981-01-15

Filing date

1982-01-14

Publication date

1984-04-18

1982-01-14 Application filed by Cpc International Inc filed Critical Cpc International Inc

1984-04-18 Publication of DD208988A5 publication Critical patent/DD208988A5/de

Links

108090000637 alpha-Amylases Proteins 0.000 title claims abstract description 58
238000000034 method Methods 0.000 title claims abstract description 49
108090000623 proteins and genes Proteins 0.000 title claims abstract description 47
102000004139 alpha-Amylases Human genes 0.000 title claims abstract description 46
229940024171 alpha-amylase Drugs 0.000 title claims abstract description 36
239000013612 plasmid Substances 0.000 claims abstract description 122
244000005700 microbiome Species 0.000 claims abstract description 34
108010065511 Amylases Proteins 0.000 claims description 48
102000013142 Amylases Human genes 0.000 claims description 37
235000019418 amylase Nutrition 0.000 claims description 36
239000004382 Amylase Substances 0.000 claims description 33
239000013598 vector Substances 0.000 claims description 25
241000588724 Escherichia coli Species 0.000 claims description 12
239000012634 fragment Substances 0.000 claims description 12
108091008146 restriction endonucleases Proteins 0.000 claims description 10
101000588924 Anthopleura elegantissima Delta-actitoxin-Ael1a Proteins 0.000 claims description 9
101150101441 dus gene Proteins 0.000 claims description 7
241000193385 Geobacillus stearothermophilus Species 0.000 claims description 5
102000003960 Ligases Human genes 0.000 claims description 5
108090000364 Ligases Proteins 0.000 claims description 5
238000003776 cleavage reaction Methods 0.000 claims description 3
230000007017 scission Effects 0.000 claims description 3
230000008569 process Effects 0.000 abstract description 4
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239000000243 solution Substances 0.000 description 13
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229960005091 chloramphenicol Drugs 0.000 description 11
WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 11
229940088598 enzyme Drugs 0.000 description 11
229920002472 Starch Polymers 0.000 description 8
229960000723 ampicillin Drugs 0.000 description 8
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 8
239000008107 starch Substances 0.000 description 8
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QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 5
241000894006 Bacteria Species 0.000 description 5
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150000003522 tetracyclines Chemical class 0.000 description 5
241000193830 Bacillus <bacterium> Species 0.000 description 4
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
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WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
229930006000 Sucrose Natural products 0.000 description 4
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
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239000008103 glucose Substances 0.000 description 4
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239000000047 product Substances 0.000 description 4
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238000012360 testing method Methods 0.000 description 4
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244000063299 Bacillus subtilis Species 0.000 description 3
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150000008163 sugars Chemical class 0.000 description 3
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238000012546 transfer Methods 0.000 description 3
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XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
229920000936 Agarose Polymers 0.000 description 2
CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 2
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-1 Casein amino acids Chemical class 0.000 description 2
229920002261 Corn starch Polymers 0.000 description 2
DYDCUQKUCUHJBH-UWTATZPHSA-N D-Cycloserine Chemical compound N[C@@H]1CONC1=O DYDCUQKUCUHJBH-UWTATZPHSA-N 0.000 description 2
DYDCUQKUCUHJBH-UHFFFAOYSA-N D-Cycloserine Natural products NC1CONC1=O DYDCUQKUCUHJBH-UHFFFAOYSA-N 0.000 description 2
230000004544 DNA amplification Effects 0.000 description 2
244000025221 Humulus lupulus Species 0.000 description 2
235000008694 Humulus lupulus Nutrition 0.000 description 2
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
108020004511 Recombinant DNA Proteins 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
239000007983 Tris buffer Substances 0.000 description 2
238000010521 absorption reaction Methods 0.000 description 2
230000008901 benefit Effects 0.000 description 2
239000007478 blood agar base Substances 0.000 description 2
AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
239000001110 calcium chloride Substances 0.000 description 2
229910001628 calcium chloride Inorganic materials 0.000 description 2
239000005018 casein Substances 0.000 description 2
235000021240 caseins Nutrition 0.000 description 2
230000002759 chromosomal effect Effects 0.000 description 2
210000000349 chromosome Anatomy 0.000 description 2
238000010367 cloning Methods 0.000 description 2
239000008120 corn starch Substances 0.000 description 2
230000009089 cytolysis Effects 0.000 description 2
238000000502 dialysis Methods 0.000 description 2
238000000605 extraction Methods 0.000 description 2
238000010353 genetic engineering Methods 0.000 description 2
239000001963 growth medium Substances 0.000 description 2
230000000977 initiatory effect Effects 0.000 description 2
238000003780 insertion Methods 0.000 description 2
230000037431 insertion Effects 0.000 description 2
230000002934 lysing effect Effects 0.000 description 2
230000001404 mediated effect Effects 0.000 description 2
238000012986 modification Methods 0.000 description 2
230000004048 modification Effects 0.000 description 2
239000003921 oil Substances 0.000 description 2
210000001938 protoplast Anatomy 0.000 description 2
238000000926 separation method Methods 0.000 description 2
239000001632 sodium acetate Substances 0.000 description 2
238000003756 stirring Methods 0.000 description 2
239000000126 substance Substances 0.000 description 2
239000006228 supernatant Substances 0.000 description 2
238000003786 synthesis reaction Methods 0.000 description 2
241000304886 Bacilli Species 0.000 description 1
241000193744 Bacillus amyloliquefaciens Species 0.000 description 1
241000194108 Bacillus licheniformis Species 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
102000053602 DNA Human genes 0.000 description 1
102100031920 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial Human genes 0.000 description 1
101000992065 Homo sapiens Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial Proteins 0.000 description 1
108091028043 Nucleic acid sequence Proteins 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
239000001888 Peptone Substances 0.000 description 1
108010080698 Peptones Proteins 0.000 description 1
102000029797 Prion Human genes 0.000 description 1
108091000054 Prion Proteins 0.000 description 1
RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical class C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
239000004809 Teflon Substances 0.000 description 1
229920006362 Teflon® Polymers 0.000 description 1
240000008042 Zea mays Species 0.000 description 1
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
235000002017 Zea mays subsp mays Nutrition 0.000 description 1
239000002253 acid Substances 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
230000009471 action Effects 0.000 description 1
238000000246 agarose gel electrophoresis Methods 0.000 description 1
150000001412 amines Chemical class 0.000 description 1
230000003321 amplification Effects 0.000 description 1
206010002022 amyloidosis Diseases 0.000 description 1
229940088710 antibiotic agent Drugs 0.000 description 1
238000003556 assay Methods 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
238000009835 boiling Methods 0.000 description 1
239000000872 buffer Substances 0.000 description 1
229910001424 calcium ion Inorganic materials 0.000 description 1
229940041514 candida albicans extract Drugs 0.000 description 1
230000006037 cell lysis Effects 0.000 description 1
239000006285 cell suspension Substances 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
239000013611 chromosomal DNA Substances 0.000 description 1
XLJKHNWPARRRJB-UHFFFAOYSA-N cobalt(2+) Chemical compound [Co+2] XLJKHNWPARRRJB-UHFFFAOYSA-N 0.000 description 1
230000000052 comparative effect Effects 0.000 description 1
230000001276 controlling effect Effects 0.000 description 1
235000005822 corn Nutrition 0.000 description 1
239000002285 corn oil Substances 0.000 description 1
235000005687 corn oil Nutrition 0.000 description 1
238000001773 deep-level transient spectroscopy Methods 0.000 description 1
238000002298 density-gradient ultracentrifugation Methods 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
230000008021 deposition Effects 0.000 description 1
IWEDIXLBFLAXBO-UHFFFAOYSA-N dicamba Chemical compound COC1=C(Cl)C=CC(Cl)=C1C(O)=O IWEDIXLBFLAXBO-UHFFFAOYSA-N 0.000 description 1
239000003814 drug Substances 0.000 description 1
229940079593 drug Drugs 0.000 description 1
210000002969 egg yolk Anatomy 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
230000007071 enzymatic hydrolysis Effects 0.000 description 1
238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
229960005542 ethidium bromide Drugs 0.000 description 1
239000012530 fluid Substances 0.000 description 1
230000004927 fusion Effects 0.000 description 1
239000000499 gel Substances 0.000 description 1
238000012239 gene modification Methods 0.000 description 1
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235000013617 genetically modified food Nutrition 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
230000007062 hydrolysis Effects 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
239000005457 ice water Substances 0.000 description 1
238000010348 incorporation Methods 0.000 description 1
238000011534 incubation Methods 0.000 description 1
238000009655 industrial fermentation Methods 0.000 description 1
238000009776 industrial production Methods 0.000 description 1
238000011081 inoculation Methods 0.000 description 1
230000003834 intracellular effect Effects 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
238000005304 joining Methods 0.000 description 1
238000011068 loading method Methods 0.000 description 1
230000007774 longterm Effects 0.000 description 1
238000005259 measurement Methods 0.000 description 1
238000003199 nucleic acid amplification method Methods 0.000 description 1
235000015097 nutrients Nutrition 0.000 description 1
235000019198 oils Nutrition 0.000 description 1
238000007500 overflow downdraw method Methods 0.000 description 1
235000019319 peptone Nutrition 0.000 description 1
239000000575 pesticide Substances 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
229940012957 plasmin Drugs 0.000 description 1
238000007747 plating Methods 0.000 description 1
239000000583 progesterone congener Substances 0.000 description 1
235000018102 proteins Nutrition 0.000 description 1
238000000746 purification Methods 0.000 description 1
230000001105 regulatory effect Effects 0.000 description 1
238000011160 research Methods 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
235000011888 snacks Nutrition 0.000 description 1
235000017281 sodium acetate Nutrition 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
239000001509 sodium citrate Substances 0.000 description 1
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
210000002325 somatostatin-secreting cell Anatomy 0.000 description 1
235000020357 syrup Nutrition 0.000 description 1
239000006188 syrup Substances 0.000 description 1
238000004809 thin layer chromatography Methods 0.000 description 1
230000001131 transforming effect Effects 0.000 description 1
239000012137 tryptone Substances 0.000 description 1
229960004799 tryptophan Drugs 0.000 description 1
235000013343 vitamin Nutrition 0.000 description 1
239000011782 vitamin Substances 0.000 description 1
229940088594 vitamin Drugs 0.000 description 1
229930003231 vitamin Natural products 0.000 description 1
150000003722 vitamin derivatives Chemical class 0.000 description 1
239000012138 yeast extract Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Zoology (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
Wood Science & Technology (AREA)
General Engineering & Computer Science (AREA)
Biochemistry (AREA)
Microbiology (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Chemical Or Physical Treatment Of Fibers (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Dental Preparations (AREA)
Bakery Products And Manufacturing Methods Therefor (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Medicinal Preparation (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Saccharide Compounds (AREA)

DD82236740A 1981-01-15 1982-01-14 Verfahren zum aufbau eines chimaeren plasmids mit einer genkodierung fuer eine thermostabile alpha-amylase DD208988A5 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US22528781A	1981-01-15	1981-01-15

Publications (1)

Publication Number	Publication Date
DD208988A5 true DD208988A5 (de)	1984-04-18

Family

ID=22844306

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DD82236740A DD208988A5 (de)	1981-01-15	1982-01-14	Verfahren zum aufbau eines chimaeren plasmids mit einer genkodierung fuer eine thermostabile alpha-amylase

Country Status (16)

Country	Link
EP (1)	EP0057976B1 (da)
JP (1)	JPS57139097A (da)
AT (1)	ATE17373T1 (da)
CA (1)	CA1170202A (da)
DD (1)	DD208988A5 (da)
DE (1)	DE3268340D1 (da)
DK (1)	DK157879C (da)
ES (2)	ES508694A0 (da)
FI (1)	FI79139C (da)
HU (1)	HU193516B (da)
IE (1)	IE52434B1 (da)
IL (1)	IL64741A (da)
MX (1)	MX7066E (da)
SU (1)	SU1200853A3 (da)
YU (2)	YU9682A (da)
ZA (1)	ZA82133B (da)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4886754A (en) *	1980-01-07	1989-12-12	The University Of Rochester	Recombinant bateriophage for heterologous cloning of bacillus microorganisms and method for its production
FR2533583A1 (fr) *	1982-09-24	1984-03-30	Centre Nat Rech Scient	Nouvel adn recombinant utile dans la preparation d'a-amylase
ATE48156T1 (de) *	1982-11-01	1989-12-15	Miles Inc	Verfahren zur heterologen klonierung eines gens in einem bacillus-mikroorganismus.
US4559300A (en) *	1983-01-18	1985-12-17	Eli Lilly And Company	Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces
JPS59196092A (ja) *	1983-04-25	1984-11-07	Sanraku Inc	組換えプラスミドによる耐熱性α−アミラ−ゼの新規製造法
IE81141B1 (en) *	1983-06-24	2000-04-05	Genencor Int	Procaryotic carbonyl hydrolases
AU587960B2 (en) *	1983-06-24	1989-09-07	Genentech Inc.	Procaryotic carbonyl hydrolases
US4578352A (en) *	1983-07-13	1986-03-25	Cpc International Inc.	Novel thermostable, aciduric alpha-amylase and method for its production
GB8333797D0 (en) *	1983-12-19	1984-01-25	Searle & Co	Starch utilization
US5032510A (en) *	1984-09-26	1991-07-16	Eli Lilly And Company	Method for expression and secretion in bacillus
EP0221923A4 (en) *	1985-03-29	1990-03-22	Bioteknika International	SECRETION VECTOR.
JPH0616711B2 (ja) *	1985-09-27	1994-03-09	メルシャン株式会社	高温で自律増殖するプラスミド
US5278059A (en) *	1985-12-04	1994-01-11	Kabushiki Kaisha Hayashibara Seibutsu Kagaki Kenkyujo	Polypeptide possessing cyclomaltodextrin glucanotransferase activity
IN165610B (da) *	1986-12-22	1989-11-25	Enzyme Bio Systems Ltd
US4977089A (en) *	1987-01-30	1990-12-11	Eli Lilly And Company	Vector comprising signal peptide-encoding DNA for use in Bacillus and other microorganisms
ATE166922T1 (de) *	1989-06-29	1998-06-15	Genencor Int	Mutierte mikrobielle alpha-amylasen mit erhöhter thermischer, saurer und/oder alkylischer stabilität
NL8902128A (nl) *	1989-08-23	1991-03-18	Avebe Coop Verkoop Prod	Vertakkingsenzym en gebruik daarvan.
US6300115B1 (en)	1998-05-18	2001-10-09	Enzyme Bio-Systems Ltd.	Pullulanase expression constructs containing α-amylase promoter and leader sequences

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
NZ183818A (en) *	1976-04-19	1980-05-08	Cpc International Inc	Heat -and acid-stable alpha-amylase and process for converting starch to a starch hydrolysate
IL61982A (en) *	1980-02-15	1984-01-31	Cpc International Inc	Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes

1982
- 1982-01-08 CA CA000393781A patent/CA1170202A/en not_active Expired
- 1982-01-08 IE IE32/82A patent/IE52434B1/en unknown
- 1982-01-08 ZA ZA82133A patent/ZA82133B/xx unknown
- 1982-01-10 IL IL64741A patent/IL64741A/xx unknown
- 1982-01-12 DE DE8282300158T patent/DE3268340D1/de not_active Expired
- 1982-01-12 AT AT82300158T patent/ATE17373T1/de not_active IP Right Cessation
- 1982-01-12 EP EP82300158A patent/EP0057976B1/en not_active Expired
- 1982-01-13 ES ES508694A patent/ES508694A0/es active Granted
- 1982-01-13 FI FI820107A patent/FI79139C/fi not_active IP Right Cessation
- 1982-01-14 DD DD82236740A patent/DD208988A5/de unknown
- 1982-01-14 DK DK013182A patent/DK157879C/da active
- 1982-01-14 SU SU823383349A patent/SU1200853A3/ru active
- 1982-01-14 MX MX829870U patent/MX7066E/es unknown
- 1982-01-14 JP JP57003522A patent/JPS57139097A/ja active Pending
- 1982-01-15 YU YU00096/82A patent/YU9682A/xx unknown
- 1982-01-15 HU HU82114A patent/HU193516B/hu not_active IP Right Cessation
- 1982-12-14 ES ES518157A patent/ES8405070A1/es not_active Expired
1984
- 1984-10-15 YU YU01763/84A patent/YU176384A/xx unknown

Also Published As

Publication number	Publication date
ES8305041A1 (es)	1983-03-16
DK157879B (da)	1990-02-26
ES508694A0 (es)	1983-03-16
ES518157A0 (es)	1984-05-16
ES8405070A1 (es)	1984-05-16
ATE17373T1 (de)	1986-01-15
YU9682A (en)	1985-06-30
EP0057976A3 (en)	1982-09-01
EP0057976A2 (en)	1982-08-18
IL64741A (en)	1985-07-31
ZA82133B (en)	1983-02-23
DE3268340D1 (en)	1986-02-20
SU1200853A3 (ru)	1985-12-23
IE820032L (en)	1982-07-15
FI79139B (fi)	1989-07-31
FI820107L (fi)	1982-07-16
IE52434B1 (en)	1987-10-28
YU176384A (en)	1987-02-28
HU193516B (en)	1987-10-28
DK13182A (da)	1982-07-16
DK157879C (da)	1990-07-30
EP0057976B1 (en)	1986-01-08
FI79139C (fi)	1989-11-10
CA1170202A (en)	1984-07-03
IL64741A0 (en)	1982-03-31
MX7066E (es)	1987-04-20
JPS57139097A (en)	1982-08-27

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