ATE166922T1 - Mutierte mikrobielle alpha-amylasen mit erhöhter thermischer, saurer und/oder alkylischer stabilität - Google Patents

Mutierte mikrobielle alpha-amylasen mit erhöhter thermischer, saurer und/oder alkylischer stabilität

Info

Publication number
ATE166922T1
ATE166922T1 AT90201706T AT90201706T ATE166922T1 AT E166922 T1 ATE166922 T1 AT E166922T1 AT 90201706 T AT90201706 T AT 90201706T AT 90201706 T AT90201706 T AT 90201706T AT E166922 T1 ATE166922 T1 AT E166922T1
Authority
AT
Austria
Prior art keywords
amylases
alpha
alkylic
acidic
stability
Prior art date
Application number
AT90201706T
Other languages
English (en)
Inventor
Wilhelmus Johannes Quax
Yves Laroche
Adrianus Wilhelmus Vollebregt
Patrick Stanssens
Marc Lauwereys
Original Assignee
Genencor Int
Plant Genetic Systems Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int, Plant Genetic Systems Nv filed Critical Genencor Int
Application granted granted Critical
Publication of ATE166922T1 publication Critical patent/ATE166922T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cigarettes, Filters, And Manufacturing Of Filters (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Tires In General (AREA)
AT90201706T 1989-06-29 1990-06-27 Mutierte mikrobielle alpha-amylasen mit erhöhter thermischer, saurer und/oder alkylischer stabilität ATE166922T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP89201735 1989-06-29

Publications (1)

Publication Number Publication Date
ATE166922T1 true ATE166922T1 (de) 1998-06-15

Family

ID=8202426

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90201706T ATE166922T1 (de) 1989-06-29 1990-06-27 Mutierte mikrobielle alpha-amylasen mit erhöhter thermischer, saurer und/oder alkylischer stabilität

Country Status (17)

Country Link
US (1) US5364782A (de)
EP (1) EP0410498B1 (de)
JP (2) JP3086249B2 (de)
KR (1) KR0165550B1 (de)
CN (1) CN1050220A (de)
AT (1) ATE166922T1 (de)
BG (1) BG61081B1 (de)
BR (1) BR9006818A (de)
CA (1) CA2030554C (de)
DD (1) DD301620A9 (de)
DE (1) DE69032360T2 (de)
DK (1) DK0410498T3 (de)
ES (1) ES2117625T3 (de)
FI (1) FI103285B1 (de)
IE (1) IE902369A1 (de)
PT (1) PT94560B (de)
WO (1) WO1991000353A2 (de)

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WO1991000353A3 (en) 1991-02-21
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BR9006818A (pt) 1991-08-06
DE69032360T2 (de) 1998-12-03
KR0165550B1 (ko) 1999-01-15
DE69032360D1 (de) 1998-07-09
PT94560A (pt) 1991-02-08
DD301620A9 (de) 1993-04-29
EP0410498B1 (de) 1998-06-03
IE902369A1 (en) 1991-06-19
DK0410498T3 (da) 1999-03-22
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