DD275882A5 - Gentechnologisches Verfahren zur Herstellung von Pflanzen mit modifizierter Blütenfarbe - Google Patents

Gentechnologisches Verfahren zur Herstellung von Pflanzen mit modifizierter Blütenfarbe Download PDF

Info

Publication number: DD275882A5
Authority: DD; German Democratic Republic
Prior art keywords: plant; dna sequence; plants; dihydrokaempferol; vector
Prior art date: 1987-11-13

Application number

DD32173188A

Other languages

German (de)

English (en)

Inventor

Peter Meyer

Iris Heidmann

Heinz Saedler

Gert Forkmann

Original Assignee

Max Planck Gesellschaft

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-11-13

Filing date

1988-11-11

Publication date

1990-02-07

1988-11-11 Application filed by Max Planck Gesellschaft filed Critical Max Planck Gesellschaft

1990-02-07 Publication of DD275882A5 publication Critical patent/DD275882A5/de

Links

238000000034 method Methods 0.000 title claims abstract description 27
238000004519 manufacturing process Methods 0.000 title claims abstract description 5
238000010353 genetic engineering Methods 0.000 title description 4
108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 20
PADQINQHPQKXNL-LSDHHAIUSA-N (+)-dihydrokaempferol Chemical compound C1([C@@H]2[C@H](C(C3=C(O)C=C(O)C=C3O2)=O)O)=CC=C(O)C=C1 PADQINQHPQKXNL-LSDHHAIUSA-N 0.000 claims abstract description 17
RAYJUFCFJUVJBB-UHFFFAOYSA-N dihydrokaempferol Natural products OC1Oc2c(O)cc(O)cc2C(=O)C1c3ccc(O)cc3 RAYJUFCFJUVJBB-UHFFFAOYSA-N 0.000 claims abstract description 17
108090000623 proteins and genes Proteins 0.000 claims abstract description 17
239000000758 substrate Substances 0.000 claims abstract description 11
102000004169 proteins and genes Human genes 0.000 claims abstract description 10
230000002255 enzymatic effect Effects 0.000 claims abstract description 9
108010044229 Dihydroflavanol 4-reductase Proteins 0.000 claims abstract description 8
241000196324 Embryophyta Species 0.000 claims description 51
239000013612 plasmid Substances 0.000 claims description 17
239000013598 vector Substances 0.000 claims description 16
240000008042 Zea mays Species 0.000 claims description 15
240000007377 Petunia x hybrida Species 0.000 claims description 14
235000007244 Zea mays Nutrition 0.000 claims description 13
210000004027 cell Anatomy 0.000 claims description 9
229930027917 kanamycin Natural products 0.000 claims description 7
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 claims description 7
229960000318 kanamycin Drugs 0.000 claims description 7
229930182823 kanamycin A Natural products 0.000 claims description 7
210000001938 protoplast Anatomy 0.000 claims description 6
230000009466 transformation Effects 0.000 claims description 6
240000001436 Antirrhinum majus Species 0.000 claims description 5
CXQWRCVTCMQVQX-LSDHHAIUSA-N (+)-taxifolin Chemical compound C1([C@@H]2[C@H](C(C3=C(O)C=C(O)C=C3O2)=O)O)=CC=C(O)C(O)=C1 CXQWRCVTCMQVQX-LSDHHAIUSA-N 0.000 claims description 4
230000027311 M phase Effects 0.000 claims description 4
XCGZWJIXHMSSQC-UHFFFAOYSA-N dihydroquercetin Natural products OC1=CC2OC(=C(O)C(=O)C2C(O)=C1)c1ccc(O)c(O)c1 XCGZWJIXHMSSQC-UHFFFAOYSA-N 0.000 claims description 4
239000003550 marker Substances 0.000 claims description 4
230000001360 synchronised effect Effects 0.000 claims description 4
238000004520 electroporation Methods 0.000 claims description 3
230000009395 genetic defect Effects 0.000 claims description 3
238000000520 microinjection Methods 0.000 claims description 3
230000009261 transgenic effect Effects 0.000 claims description 3
238000002360 preparation method Methods 0.000 claims description 2
108090000790 Enzymes Proteins 0.000 description 10
102000004190 Enzymes Human genes 0.000 description 10
229930002877 anthocyanin Natural products 0.000 description 9
235000010208 anthocyanin Nutrition 0.000 description 9
239000004410 anthocyanin Substances 0.000 description 9
150000004636 anthocyanins Chemical class 0.000 description 9
239000000975 dye Substances 0.000 description 8
101150008391 A1 gene Proteins 0.000 description 7
241000972773 Aulopiformes Species 0.000 description 7
239000002299 complementary DNA Substances 0.000 description 7
235000019515 salmon Nutrition 0.000 description 7
230000015572 biosynthetic process Effects 0.000 description 6
SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 6
101150084750 1 gene Proteins 0.000 description 5
241000701489 Cauliflower mosaic virus Species 0.000 description 5
108020004414 DNA Proteins 0.000 description 5
241000207748 Petunia Species 0.000 description 5
239000012634 fragment Substances 0.000 description 5
FSVMLWOLZHGCQX-UHFFFAOYSA-N Leucopelargonidin Chemical compound OC1C(O)C2=C(O)C=C(O)C=C2OC1C1=CC=C(O)C=C1 FSVMLWOLZHGCQX-UHFFFAOYSA-N 0.000 description 4
230000000694 effects Effects 0.000 description 4
230000002068 genetic effect Effects 0.000 description 4
IYRMWMYZSQPJKC-UHFFFAOYSA-N kaempferol Chemical compound C1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 IYRMWMYZSQPJKC-UHFFFAOYSA-N 0.000 description 4
239000002609 medium Substances 0.000 description 4
238000003786 synthesis reaction Methods 0.000 description 4
238000013518 transcription Methods 0.000 description 4
230000035897 transcription Effects 0.000 description 4
NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 3
238000004458 analytical method Methods 0.000 description 3
238000010276 construction Methods 0.000 description 3
150000001915 cyanidin derivatives Chemical class 0.000 description 3
150000001959 delphinidin derivatives Chemical class 0.000 description 3
239000003617 indole-3-acetic acid Substances 0.000 description 3
239000000543 intermediate Substances 0.000 description 3
KJXSIXMJHKAJOD-LSDHHAIUSA-N (+)-dihydromyricetin Chemical compound C1([C@@H]2[C@H](C(C3=C(O)C=C(O)C=C3O2)=O)O)=CC(O)=C(O)C(O)=C1 KJXSIXMJHKAJOD-LSDHHAIUSA-N 0.000 description 2
241000207875 Antirrhinum Species 0.000 description 2
UBSCDKPKWHYZNX-UHFFFAOYSA-N Demethoxycapillarisin Natural products C1=CC(O)=CC=C1OC1=CC(=O)C2=C(O)C=C(O)C=C2O1 UBSCDKPKWHYZNX-UHFFFAOYSA-N 0.000 description 2
FSVMLWOLZHGCQX-SOUVJXGZSA-N Leucopelargonidin Natural products C1([C@H]2OC3=CC(O)=CC(O)=C3[C@H](O)[C@@H]2O)=CC=C(O)C=C1 FSVMLWOLZHGCQX-SOUVJXGZSA-N 0.000 description 2
244000061322 Nicotiana alata Species 0.000 description 2
241000209149 Zea Species 0.000 description 2
101100443098 Zea mays A1 gene Proteins 0.000 description 2
230000006696 biosynthetic metabolic pathway Effects 0.000 description 2
238000009395 breeding Methods 0.000 description 2
230000001488 breeding effect Effects 0.000 description 2
VEVZSMAEJFVWIL-UHFFFAOYSA-O cyanidin cation Chemical compound [O+]=1C2=CC(O)=CC(O)=C2C=C(O)C=1C1=CC=C(O)C(O)=C1 VEVZSMAEJFVWIL-UHFFFAOYSA-O 0.000 description 2
230000007547 defect Effects 0.000 description 2
230000007812 deficiency Effects 0.000 description 2
229930003935 flavonoid Natural products 0.000 description 2
150000002215 flavonoids Chemical class 0.000 description 2
235000017173 flavonoids Nutrition 0.000 description 2
229930182470 glycoside Natural products 0.000 description 2
150000002338 glycosides Chemical class 0.000 description 2
238000005805 hydroxylation reaction Methods 0.000 description 2
MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 2
235000008777 kaempferol Nutrition 0.000 description 2
108020004999 messenger RNA Proteins 0.000 description 2
UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 description 2
239000006870 ms-medium Substances 0.000 description 2
HKUHOPQRJKPJCJ-UHFFFAOYSA-N pelargonidin Natural products OC1=Cc2c(O)cc(O)cc2OC1c1ccc(O)cc1 HKUHOPQRJKPJCJ-UHFFFAOYSA-N 0.000 description 2
235000006251 pelargonidin Nutrition 0.000 description 2
XVFMGWDSJLBXDZ-UHFFFAOYSA-O pelargonidin Chemical compound C1=CC(O)=CC=C1C(C(=C1)O)=[O+]C2=C1C(O)=CC(O)=C2 XVFMGWDSJLBXDZ-UHFFFAOYSA-O 0.000 description 2
238000012546 transfer Methods 0.000 description 2
OSDLLIBGSJNGJE-UHFFFAOYSA-N 4-chloro-3,5-dimethylphenol Chemical compound CC1=CC(O)=CC(C)=C1Cl OSDLLIBGSJNGJE-UHFFFAOYSA-N 0.000 description 1
241000132092 Aster Species 0.000 description 1
101150002919 DFR gene Proteins 0.000 description 1
MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
OEIJRRGCTVHYTH-UHFFFAOYSA-N Favan-3-ol Chemical compound OC1CC2=CC=CC=C2OC1C1=CC=CC=C1 OEIJRRGCTVHYTH-UHFFFAOYSA-N 0.000 description 1
CITFYDYEWQIEPX-UHFFFAOYSA-N Flavanol Natural products O1C2=CC(OCC=C(C)C)=CC(O)=C2C(=O)C(O)C1C1=CC=C(O)C=C1 CITFYDYEWQIEPX-UHFFFAOYSA-N 0.000 description 1
108010062650 Flavonoid 3',5'-hydroxylase Proteins 0.000 description 1
206010020649 Hyperkeratosis Diseases 0.000 description 1
241000209510 Liliopsida Species 0.000 description 1
244000088413 Matthiola incana Species 0.000 description 1
235000011378 Matthiola incana Nutrition 0.000 description 1
238000000636 Northern blotting Methods 0.000 description 1
108020004511 Recombinant DNA Proteins 0.000 description 1
108700025674 Zea mays A1 Proteins 0.000 description 1
101001044937 Zea mays Dihydroflavonol 4-reductase Proteins 0.000 description 1
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
235000002017 Zea mays subsp mays Nutrition 0.000 description 1
108010050181 aleurone Proteins 0.000 description 1
239000008280 blood Substances 0.000 description 1
210000004369 blood Anatomy 0.000 description 1
239000003086 colorant Substances 0.000 description 1
230000000052 comparative effect Effects 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
235000005822 corn Nutrition 0.000 description 1
238000009402 cross-breeding Methods 0.000 description 1
235000007336 cyanidin Nutrition 0.000 description 1
KQILIWXGGKGKNX-UHFFFAOYSA-N dihydromyricetin Natural products OC1C(=C(Oc2cc(O)cc(O)c12)c3cc(O)c(O)c(O)c3)O KQILIWXGGKGKNX-UHFFFAOYSA-N 0.000 description 1
229910001882 dioxygen Inorganic materials 0.000 description 1
235000011987 flavanols Nutrition 0.000 description 1
102000037865 fusion proteins Human genes 0.000 description 1
108020001507 fusion proteins Proteins 0.000 description 1
239000005556 hormone Substances 0.000 description 1
229940088597 hormone Drugs 0.000 description 1
238000009396 hybridization Methods 0.000 description 1
230000033444 hydroxylation Effects 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
239000002502 liposome Substances 0.000 description 1
238000010841 mRNA extraction Methods 0.000 description 1
230000001404 mediated effect Effects 0.000 description 1
244000005700 microbiome Species 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
239000002674 ointment Substances 0.000 description 1
238000003976 plant breeding Methods 0.000 description 1
230000008929 regeneration Effects 0.000 description 1
238000011069 regeneration method Methods 0.000 description 1
239000000523 sample Substances 0.000 description 1
241000894007 species Species 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
239000000126 substance Substances 0.000 description 1
230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01219—Dihydrokaempferol 4-reductase (1.1.1.219), i.e. dihydroflavonol-4-reductase

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Biomedical Technology (AREA)
Microbiology (AREA)
Medicinal Chemistry (AREA)
Nutrition Science (AREA)
Cell Biology (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

DD32173188A 1987-11-13 1988-11-11 Gentechnologisches Verfahren zur Herstellung von Pflanzen mit modifizierter Blütenfarbe DD275882A5 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE3738657A DE3738657C1 (de)	1987-11-13	1987-11-13	Pflanzen mit modifizierter Bluetenfarbe und gentechnologische Verfahren zu ihrer Herstellung

Publications (1)

Publication Number	Publication Date
DD275882A5 true DD275882A5 (de)	1990-02-07

Family

ID=6340474

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DD32173188A DD275882A5 (de)	1987-11-13	1988-11-11	Gentechnologisches Verfahren zur Herstellung von Pflanzen mit modifizierter Blütenfarbe

Country Status (7)

Country	Link
US (1)	US5410096A (fr)
EP (1)	EP0316797B1 (fr)
JP (1)	JP2996663B2 (fr)
DD (1)	DD275882A5 (fr)
DE (2)	DE3738657C1 (fr)
ES (1)	ES2059472T3 (fr)
IL (1)	IL88307A (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
PL170326B1 (pl) *	1991-07-11	1996-11-29	Int Flower Dev Pty Ltd	S posób wytwarzania rosliny transgenicznej PL
AUPN298895A0 (en) *	1995-05-16	1995-06-08	International Flower Developments Pty Ltd	Transgenic plants exhibiting altered flower colour and methods for producing same
AU699874B2 (en) *	1995-05-16	1998-12-17	International Flower Developments Pty Ltd	Transgenic plants exhibiting altered flower colour and methods for producing same
US6459017B1 (en)	2000-06-29	2002-10-01	The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon State University	Iris transformation method
US6465630B1 (en) *	2000-08-14	2002-10-15	Korea Kumho Petrochemical Co. Ltd.	Genetic sequences encoding substrate-specific dihydroflavonol 4-reductase and uses therefor
US20040117871A1 (en) *	2000-11-25	2004-06-17	Peter Meyer	Plant growth regulation
AUPR811301A0 (en)	2001-10-05	2001-10-25	Agresearch Limited	Manipulation of flavonoid biosynthesis
KR102081982B1 (ko) *	2018-11-16	2020-02-26	대한민국	하얀 꽃색 국화 판별용 마커

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4771002A (en) *	1984-02-24	1988-09-13	Lubrizol Genetics, Inc.	Transcription in plants and bacteria

1987
- 1987-11-13 DE DE3738657A patent/DE3738657C1/de not_active Expired
1988
- 1988-11-07 IL IL88307A patent/IL88307A/xx not_active IP Right Cessation
- 1988-11-11 EP EP88118820A patent/EP0316797B1/fr not_active Expired - Lifetime
- 1988-11-11 JP JP63286605A patent/JP2996663B2/ja not_active Expired - Fee Related
- 1988-11-11 DE DE88118820T patent/DE3885061D1/de not_active Expired - Lifetime
- 1988-11-11 ES ES88118820T patent/ES2059472T3/es not_active Expired - Lifetime
- 1988-11-11 DD DD32173188A patent/DD275882A5/de not_active IP Right Cessation
1990
- 1990-08-28 US US07/573,876 patent/US5410096A/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
JPH022305A (ja)	1990-01-08
DE3885061D1 (de)	1993-11-25
IL88307A (en)	1993-08-18
EP0316797A3 (en)	1990-01-31
EP0316797A2 (fr)	1989-05-24
EP0316797B1 (fr)	1993-10-20
IL88307A0 (en)	1989-06-30
US5410096A (en)	1995-04-25
DE3738657C1 (de)	1989-05-18
JP2996663B2 (ja)	2000-01-11
ES2059472T3 (es)	1994-11-16

Legal Events

Date	Code	Title	Description
1995-09-28	ENJ	Ceased due to non-payment of renewal fee

Publication	Publication Date	Title
DE3751466T2 (de)	1996-01-18	Anti-sense-Regulierung von Pflanzengenexpression.
EP0375092B1 (fr)	1996-01-24	Régulation de transcription spécifique pour des tubercules de pomme de terre
DE69020151T2 (de)	1995-09-28	Genetisch geformte neue pflanzenphenotypen.
DE3687705T2 (de)	1993-07-08	Molekulare zuechtung.
DE69735580T2 (de)	2007-02-01	An aminosaeure angereicherte speicherproteine, insbesondere an lysin angereicherte mais-gamma-zeine, und diese exprimierende pflanzen
DE69231875T2 (de)	2001-10-04	Pflanzen-promoter involviert in der kontrolle der lipid-biosynthese in samen
DE68918533T2 (de)	1995-03-30	Überexpression von Phytochrom in transgenen Pflanzen.
EP0270496B1 (fr)	1993-03-17	Méthode pour la transformation de protoplastes végétaux
DE69434312T2 (de)	2006-03-30	Regulierung von pflanzenwachstum
DE69332538T2 (de)	2003-11-06	Cis elemente des ölkörperproteins als regulationische signale
DE4004800C2 (de)	2000-12-21	Im Habitus und Ertrag veränderte transgene Pflanzen
DE68925258T2 (de)	1996-05-15	Transgene pflanze mit veränderter physiologie, morphologie und hormonstoffwechsel gewebekulturen dieser pflanze sowie verfahren zu ihrer herstellung
DE69731608T2 (de)	2006-03-23	Verbessertes barstar-gen
DE69832489T2 (de)	2006-07-20	Induktion von männlicher sterilität in pflanzen durch erhöhte expression von streptavidin
DE4441408A1 (de)	1996-05-15	DNA-Sequenzen aus Solanum tuberosum kodierend Enzyme, die an der Stärkesynthese beteiligt sind, Plasmide, Bakterien, Pflanzenzellen und transgene Pflanzen enhaltend diese Sequenzen
DE69432796T2 (de)	2004-04-29	Kombination von dna-sequenzen, die die bildung von modifizierter stärke in pflanzenzellen und pflanzen ermöglicht, verfahren zur herstellung dieser pflanzen
DD242425A5 (de)	1987-01-28	Verfahren zur Transformation pflanzlichen Erbgutes
DE69132758T2 (de)	2002-09-05	Plasmide zur Herstellung von transgenen Pflanzen welche in Habitus und Ertrag verändert sind
DE69433424T2 (de)	2004-09-30	Pflanzen mit modifizierter reaktion auf ethylen
DE69333476T2 (de)	2004-08-12	Verfahren zur Herstellung von Proteinen in Pflanzenflüssigkeiten
DE69332951T2 (de)	2004-02-26	Gensequenzen kodierend für enzyme des flavonoid stoffwechselweges mit einer flavonoid 3'-hydroxylase aktivität une ihre anwendungen
DE69839178T2 (de)	2009-02-19	Gene, die für proteine mit transglykolysierungsaktivität kodieren.
DE69326770T2 (de)	2000-09-07	Für einen amnosäauretransporter kodierende dna-sequenzen, plasmide, bakterien,hefen, und pflanzen die einer transporter enthalten sowie ihre verwendung
DD275882A5 (de)	1990-02-07	Gentechnologisches Verfahren zur Herstellung von Pflanzen mit modifizierter Blütenfarbe
DE69333227T2 (de)	2004-08-19	Für das enzym glykosyltransferase kodierende genetische sequenzen und ihre verwendung