DD279741A5 - Verfahren zur herstellung einer an ein unloesliches traegermaterial gebundenen, spezifisch bindefaehige proteinsubstanz - Google Patents

Verfahren zur herstellung einer an ein unloesliches traegermaterial gebundenen, spezifisch bindefaehige proteinsubstanz Download PDF

Info

Publication number: DD279741A5
Authority: DD; German Democratic Republic
Prior art keywords: protein; molecular weight; substance; hydrophobic; solid phase
Prior art date: 1986-11-26

Application number

DD87309268A

Other languages

German (de)

English (en)

Inventor

Wilhelm Tischer

Josef Maier

Rolf Deeg

Original Assignee

��@��@��k��

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1986-11-26

Filing date

1987-11-20

Publication date

1990-06-13

1987-11-20 Application filed by ��@��@��k�� filed Critical ��@��@��k��

1990-06-13 Publication of DD279741A5 publication Critical patent/DD279741A5/de

Links

102000004169 proteins and genes Human genes 0.000 title claims abstract description 73
108090000623 proteins and genes Proteins 0.000 title claims abstract description 73
239000000126 substance Substances 0.000 title claims abstract description 44
238000000034 method Methods 0.000 title claims description 32
230000009870 specific binding Effects 0.000 title claims description 9
239000000463 material Substances 0.000 title claims description 7
238000004519 manufacturing process Methods 0.000 title description 5
230000027455 binding Effects 0.000 claims abstract description 30
239000007790 solid phase Substances 0.000 claims abstract description 30
230000002209 hydrophobic effect Effects 0.000 claims abstract description 22
238000003018 immunoassay Methods 0.000 claims abstract description 11
239000000376 reactant Substances 0.000 claims abstract description 11
238000010168 coupling process Methods 0.000 claims abstract description 7
230000008878 coupling Effects 0.000 claims abstract description 6
238000005859 coupling reaction Methods 0.000 claims abstract description 6
108010090804 Streptavidin Proteins 0.000 claims description 26
239000012876 carrier material Substances 0.000 claims description 14
239000004793 Polystyrene Substances 0.000 claims description 13
229920002223 polystyrene Polymers 0.000 claims description 13
238000002360 preparation method Methods 0.000 claims description 13
150000001875 compounds Chemical class 0.000 claims description 11
108091003079 Bovine Serum Albumin Proteins 0.000 claims description 7
229940098773 bovine serum albumin Drugs 0.000 claims description 6
239000003153 chemical reaction reagent Substances 0.000 claims description 6
230000008569 process Effects 0.000 claims description 6
239000002253 acid Substances 0.000 claims description 5
239000003795 chemical substances by application Substances 0.000 claims description 5
102000004882 Lipase Human genes 0.000 claims description 4
108090001060 Lipase Proteins 0.000 claims description 4
239000004367 Lipase Substances 0.000 claims description 4
PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims description 4
150000007513 acids Chemical class 0.000 claims description 4
230000003196 chaotropic effect Effects 0.000 claims description 4
239000003365 glass fiber Substances 0.000 claims description 4
150000002500 ions Chemical class 0.000 claims description 4
235000019421 lipase Nutrition 0.000 claims description 4
238000004458 analytical method Methods 0.000 claims description 3
239000000427 antigen Substances 0.000 claims description 3
102000036639 antigens Human genes 0.000 claims description 3
108091007433 antigens Proteins 0.000 claims description 3
239000001913 cellulose Substances 0.000 claims description 3
229920002678 cellulose Polymers 0.000 claims description 3
229920001577 copolymer Polymers 0.000 claims description 3
NLHHRLWOUZZQLW-UHFFFAOYSA-N Acrylonitrile Chemical compound C=CC#N NLHHRLWOUZZQLW-UHFFFAOYSA-N 0.000 claims description 2
108090001008 Avidin Proteins 0.000 claims description 2
239000004952 Polyamide Substances 0.000 claims description 2
239000004809 Teflon Substances 0.000 claims description 2
229920006362 Teflon® Polymers 0.000 claims description 2
238000012412 chemical coupling Methods 0.000 claims description 2
229920002647 polyamide Polymers 0.000 claims description 2
229920005594 polymer fiber Polymers 0.000 claims description 2
229920000193 polymethacrylate Polymers 0.000 claims description 2
230000001588 bifunctional effect Effects 0.000 claims 1
239000000243 solution Substances 0.000 description 16
102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 14
108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 14
LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 14
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 12
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
238000006243 chemical reaction Methods 0.000 description 12
238000012360 testing method Methods 0.000 description 11
238000011088 calibration curve Methods 0.000 description 10
238000004132 cross linking Methods 0.000 description 10
239000008057 potassium phosphate buffer Substances 0.000 description 10
108010005774 beta-Galactosidase Proteins 0.000 description 9
102000005936 beta-Galactosidase Human genes 0.000 description 7
229910000160 potassium phosphate Inorganic materials 0.000 description 7
235000011009 potassium phosphates Nutrition 0.000 description 7
229960002685 biotin Drugs 0.000 description 6
235000020958 biotin Nutrition 0.000 description 6
239000011616 biotin Substances 0.000 description 6
239000000872 buffer Substances 0.000 description 6
239000003599 detergent Substances 0.000 description 6
239000011780 sodium chloride Substances 0.000 description 6
108060003951 Immunoglobulin Proteins 0.000 description 5
OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 5
102000018358 immunoglobulin Human genes 0.000 description 5
239000004033 plastic Substances 0.000 description 5
239000000047 product Substances 0.000 description 5
WQZGKKKJIJFFOK-FPRJBGLDSA-N beta-D-galactose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-FPRJBGLDSA-N 0.000 description 4
230000000694 effects Effects 0.000 description 4
230000001900 immune effect Effects 0.000 description 4
238000011068 loading method Methods 0.000 description 4
230000014759 maintenance of location Effects 0.000 description 4
239000000758 substrate Substances 0.000 description 4
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
PCDWFBFHIIKIPM-UHFFFAOYSA-N 3-ethyl-2h-1,3-benzothiazole-2-sulfonic acid Chemical compound C1=CC=C2N(CC)C(S(O)(=O)=O)SC2=C1 PCDWFBFHIIKIPM-UHFFFAOYSA-N 0.000 description 3
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
238000002835 absorbance Methods 0.000 description 3
230000000274 adsorptive effect Effects 0.000 description 3
238000004587 chromatography analysis Methods 0.000 description 3
125000000524 functional group Chemical group 0.000 description 3
238000011534 incubation Methods 0.000 description 3
230000003993 interaction Effects 0.000 description 3
238000000926 separation method Methods 0.000 description 3
238000001179 sorption measurement Methods 0.000 description 3
239000012086 standard solution Substances 0.000 description 3
238000003756 stirring Methods 0.000 description 3
RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 2
FWEOQOXTVHGIFQ-UHFFFAOYSA-N 8-anilinonaphthalene-1-sulfonic acid Chemical compound C=12C(S(=O)(=O)O)=CC=CC2=CC=CC=1NC1=CC=CC=C1 FWEOQOXTVHGIFQ-UHFFFAOYSA-N 0.000 description 2
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 2
102000006395 Globulins Human genes 0.000 description 2
108010044091 Globulins Proteins 0.000 description 2
108091006905 Human Serum Albumin Proteins 0.000 description 2
102000008100 Human Serum Albumin Human genes 0.000 description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
241001362551 Samba Species 0.000 description 2
CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
102000004338 Transferrin Human genes 0.000 description 2
108090000901 Transferrin Proteins 0.000 description 2
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
239000011324 bead Substances 0.000 description 2
238000001212 derivatisation Methods 0.000 description 2
XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 2
229910000397 disodium phosphate Inorganic materials 0.000 description 2
235000019800 disodium phosphate Nutrition 0.000 description 2
ZWIBGKZDAWNIFC-UHFFFAOYSA-N disuccinimidyl suberate Chemical compound O=C1CCC(=O)N1OC(=O)CCCCCCC(=O)ON1C(=O)CCC1=O ZWIBGKZDAWNIFC-UHFFFAOYSA-N 0.000 description 2
230000002255 enzymatic effect Effects 0.000 description 2
239000012634 fragment Substances 0.000 description 2
108010074605 gamma-Globulins Proteins 0.000 description 2
238000002523 gelfiltration Methods 0.000 description 2
JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
238000005259 measurement Methods 0.000 description 2
239000000203 mixture Substances 0.000 description 2
229920000642 polymer Polymers 0.000 description 2
239000000523 sample Substances 0.000 description 2
239000007787 solid Substances 0.000 description 2
KZNICNPSHKQLFF-UHFFFAOYSA-N succinimide Chemical compound O=C1CCC(=O)N1 KZNICNPSHKQLFF-UHFFFAOYSA-N 0.000 description 2
239000006228 supernatant Substances 0.000 description 2
239000012581 transferrin Substances 0.000 description 2
238000000108 ultra-filtration Methods 0.000 description 2
102000002572 Alpha-Globulins Human genes 0.000 description 1
108010068307 Alpha-Globulins Proteins 0.000 description 1
206010003445 Ascites Diseases 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
102000014914 Carrier Proteins Human genes 0.000 description 1
241001137251 Corvidae Species 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
108090000288 Glycoproteins Proteins 0.000 description 1
102000003886 Glycoproteins Human genes 0.000 description 1
101001060288 Homo sapiens Alpha-2-HS-glycoprotein Proteins 0.000 description 1
AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 description 1
102000018071 Immunoglobulin Fc Fragments Human genes 0.000 description 1
108010091135 Immunoglobulin Fc Fragments Proteins 0.000 description 1
102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
BVHLGVCQOALMSV-JEDNCBNOSA-N L-lysine hydrochloride Chemical compound Cl.NCCCC[C@H](N)C(O)=O BVHLGVCQOALMSV-JEDNCBNOSA-N 0.000 description 1
102000007330 LDL Lipoproteins Human genes 0.000 description 1
108010007622 LDL Lipoproteins Proteins 0.000 description 1
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
239000004472 Lysine Substances 0.000 description 1
PEEHTFAAVSWFBL-UHFFFAOYSA-N Maleimide Chemical compound O=C1NC(=O)C=C1 PEEHTFAAVSWFBL-UHFFFAOYSA-N 0.000 description 1
108090000526 Papain Proteins 0.000 description 1
229920001213 Polysorbate 20 Polymers 0.000 description 1
239000005819 Potassium phosphonate Substances 0.000 description 1
239000004365 Protease Substances 0.000 description 1
229920005654 Sephadex Polymers 0.000 description 1
239000012507 Sephadex™ Substances 0.000 description 1
229930006000 Sucrose Natural products 0.000 description 1
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
235000011054 acetic acid Nutrition 0.000 description 1
230000009471 action Effects 0.000 description 1
230000004913 activation Effects 0.000 description 1
125000003172 aldehyde group Chemical group 0.000 description 1
125000000217 alkyl group Chemical group 0.000 description 1
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
229910052921 ammonium sulfate Inorganic materials 0.000 description 1
239000001166 ammonium sulphate Substances 0.000 description 1
235000011130 ammonium sulphate Nutrition 0.000 description 1
235000015278 beef Nutrition 0.000 description 1
230000008901 benefit Effects 0.000 description 1
239000011230 binding agent Substances 0.000 description 1
108091008324 binding proteins Proteins 0.000 description 1
150000003842 bromide salts Chemical class 0.000 description 1
239000004202 carbamide Substances 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
239000007979 citrate buffer Substances 0.000 description 1
238000003776 cleavage reaction Methods 0.000 description 1
239000011248 coating agent Substances 0.000 description 1
238000000576 coating method Methods 0.000 description 1
230000000052 comparative effect Effects 0.000 description 1
239000007822 coupling agent Substances 0.000 description 1
239000008367 deionised water Substances 0.000 description 1
229910021641 deionized water Inorganic materials 0.000 description 1
235000014113 dietary fatty acids Nutrition 0.000 description 1
YXXXKCDYKKSZHL-UHFFFAOYSA-M dipotassium;dioxido(oxo)phosphanium Chemical compound [K+].[K+].[O-][P+]([O-])=O YXXXKCDYKKSZHL-UHFFFAOYSA-M 0.000 description 1
239000000975 dye Substances 0.000 description 1
239000003480 eluent Substances 0.000 description 1
238000010828 elution Methods 0.000 description 1
229940088598 enzyme Drugs 0.000 description 1
238000011156 evaluation Methods 0.000 description 1
230000007717 exclusion Effects 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
239000000194 fatty acid Substances 0.000 description 1
229930195729 fatty acid Natural products 0.000 description 1
150000004665 fatty acids Chemical class 0.000 description 1
239000012530 fluid Substances 0.000 description 1
150000004673 fluoride salts Chemical class 0.000 description 1
-1 for example Substances 0.000 description 1
238000011990 functional testing Methods 0.000 description 1
238000005227 gel permeation chromatography Methods 0.000 description 1
239000011521 glass Substances 0.000 description 1
239000008187 granular material Substances 0.000 description 1
229960000789 guanidine hydrochloride Drugs 0.000 description 1
PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
102000055634 human AHSG Human genes 0.000 description 1
235000011167 hydrochloric acid Nutrition 0.000 description 1
230000037189 immune system physiology Effects 0.000 description 1
229940127121 immunoconjugate Drugs 0.000 description 1
150000004694 iodide salts Chemical class 0.000 description 1
238000004255 ion exchange chromatography Methods 0.000 description 1
238000002372 labelling Methods 0.000 description 1
239000004310 lactic acid Substances 0.000 description 1
235000014655 lactic acid Nutrition 0.000 description 1
150000002632 lipids Chemical class 0.000 description 1
239000007788 liquid Substances 0.000 description 1
239000012160 loading buffer Substances 0.000 description 1
229960003646 lysine Drugs 0.000 description 1
229910001629 magnesium chloride Inorganic materials 0.000 description 1
MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
238000002156 mixing Methods 0.000 description 1
229910000403 monosodium phosphate Inorganic materials 0.000 description 1
235000019799 monosodium phosphate Nutrition 0.000 description 1
229910052757 nitrogen Inorganic materials 0.000 description 1
239000004745 nonwoven fabric Substances 0.000 description 1
229940055729 papain Drugs 0.000 description 1
235000019834 papain Nutrition 0.000 description 1
VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical class OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 1
102000013415 peroxidase activity proteins Human genes 0.000 description 1
108040007629 peroxidase activity proteins Proteins 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
235000015108 pies Nutrition 0.000 description 1
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
229920001451 polypropylene glycol Polymers 0.000 description 1
239000000843 powder Substances 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
235000019260 propionic acid Nutrition 0.000 description 1
238000000746 purification Methods 0.000 description 1
238000010791 quenching Methods 0.000 description 1
230000000171 quenching effect Effects 0.000 description 1
IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
230000035484 reaction time Effects 0.000 description 1
AHTFMWCHTGEJHA-UHFFFAOYSA-N s-(2,5-dioxooxolan-3-yl) ethanethioate Chemical compound CC(=O)SC1CC(=O)OC1=O AHTFMWCHTGEJHA-UHFFFAOYSA-N 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
239000012488 sample solution Substances 0.000 description 1
230000007017 scission Effects 0.000 description 1
229910000029 sodium carbonate Inorganic materials 0.000 description 1
AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
239000006104 solid solution Substances 0.000 description 1
229960002317 succinimide Drugs 0.000 description 1
239000005720 sucrose Substances 0.000 description 1
150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
229920001059 synthetic polymer Polymers 0.000 description 1
238000010998 test method Methods 0.000 description 1
150000003567 thiocyanates Chemical class 0.000 description 1
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
238000005406 washing Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
- G01N33/552—Glass or silica
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Immunology (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Molecular Biology (AREA)
Hematology (AREA)
Urology & Nephrology (AREA)
Biomedical Technology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Analytical Chemistry (AREA)
Microbiology (AREA)
Food Science & Technology (AREA)
Cell Biology (AREA)
Biotechnology (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Physics & Mathematics (AREA)
Endocrinology (AREA)
Organic Chemistry (AREA)
Inorganic Chemistry (AREA)
Biophysics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Genetics & Genomics (AREA)
Peptides Or Proteins (AREA)
Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Formation And Processing Of Food Products (AREA)
Press Drives And Press Lines (AREA)
Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

DD87309268A 1986-11-26 1987-11-20 Verfahren zur herstellung einer an ein unloesliches traegermaterial gebundenen, spezifisch bindefaehige proteinsubstanz DD279741A5 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE19863640412 DE3640412A1 (de)	1986-11-26	1986-11-26	Verfahren zur bestimmung einer spezifisch bindefaehigen substanz

Publications (1)

Publication Number	Publication Date
DD279741A5 true DD279741A5 (de)	1990-06-13

Family

ID=6314821

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DD87309268A DD279741A5 (de)	1986-11-26	1987-11-20	Verfahren zur herstellung einer an ein unloesliches traegermaterial gebundenen, spezifisch bindefaehige proteinsubstanz

Country Status (13)

Country	Link
US (1)	US5061640A (ja)
EP (1)	EP0269092B1 (ja)
JP (1)	JPH0731205B2 (ja)
KR (1)	KR910002543B1 (ja)
CN (1)	CN1032164C (ja)
AT (1)	ATE86743T1 (ja)
AU (1)	AU592910B2 (ja)
CA (1)	CA1302245C (ja)
DD (1)	DD279741A5 (ja)
DE (2)	DE3640412A1 (ja)
ES (1)	ES2054648T3 (ja)
GR (1)	GR3007910T3 (ja)
ZA (1)	ZA878824B (ja)

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TR201904903T4 (tr)	2015-03-25	2019-05-21	Alexion Pharma Inc	Alternatif tamamlama yolağının C3 ve C5 konvertazının proteaz aktivitesinin ölçülmesine yönelik bir yöntem.
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1986
- 1986-11-26 DE DE19863640412 patent/DE3640412A1/de not_active Withdrawn
1987
- 1987-11-05 CA CA000551119A patent/CA1302245C/en not_active Expired - Lifetime
- 1987-11-20 DD DD87309268A patent/DD279741A5/de not_active IP Right Cessation
- 1987-11-24 US US07/124,871 patent/US5061640A/en not_active Expired - Lifetime
- 1987-11-25 CN CN87107982A patent/CN1032164C/zh not_active Expired - Fee Related
- 1987-11-25 JP JP62295432A patent/JPH0731205B2/ja not_active Expired - Lifetime
- 1987-11-25 DE DE8787117411T patent/DE3784638D1/de not_active Expired - Lifetime
- 1987-11-25 AT AT87117411T patent/ATE86743T1/de not_active IP Right Cessation
- 1987-11-25 ES ES87117411T patent/ES2054648T3/es not_active Expired - Lifetime
- 1987-11-25 AU AU81695/87A patent/AU592910B2/en not_active Ceased
- 1987-11-25 EP EP87117411A patent/EP0269092B1/de not_active Expired - Lifetime
- 1987-11-25 ZA ZA878824A patent/ZA878824B/xx unknown
- 1987-11-26 KR KR1019870013351A patent/KR910002543B1/ko not_active Expired
1993
- 1993-05-19 GR GR920403238T patent/GR3007910T3/el unknown

Also Published As

Publication number	Publication date
DE3640412A1 (de)	1988-06-09
KR890002227A (ko)	1989-04-10
AU592910B2 (en)	1990-01-25
ES2054648T3 (es)	1994-08-16
JPH0731205B2 (ja)	1995-04-10
EP0269092A2 (de)	1988-06-01
EP0269092A3 (en)	1990-01-24
CA1302245C (en)	1992-06-02
CN1032164C (zh)	1996-06-26
AU8169587A (en)	1988-06-02
KR910002543B1 (ko)	1991-04-23
CN87107982A (zh)	1988-06-15
DE3784638D1 (de)	1993-04-15
US5061640A (en)	1991-10-29
ATE86743T1 (de)	1993-03-15
JPS63142268A (ja)	1988-06-14
EP0269092B1 (de)	1993-03-10
GR3007910T3 (ja)	1993-08-31
ZA878824B (en)	1989-04-26

Legal Events

Date	Code	Title	Description
1997-09-11	ENJ	Ceased due to non-payment of renewal fee

Publication	Publication Date	Title
EP0269092B1 (de)	1993-03-10	Verfahren zur Bestimmung einer spezifisch bindefähigen Substanz
DE3105768C2 (de)	1986-08-21	Verwendung eines Trägers für die Immobilisierung von bioaktiven Materialien
DE3650513T2 (de)	1997-09-04	Verzögerter immunologischer Test in fester Phase
EP0397113B1 (de)	1993-11-18	Verfahren zum Nachweis spezifisch bindefähiger Substanzen in Körperflüssigkeiten
EP0175973B1 (de)	1989-11-08	Trägermaterial zur Verwendung für Immunbestimmungen
EP0484472B1 (de)	1997-07-16	Verfahren zur lichtinduzierten immobilisierung von biomolekülen an chemisch "inerten" oberflächen
EP0407904B1 (de)	1994-09-21	Verfahren zur Bestimmung eines Analyten
DE3842700A1 (de)	1990-06-21	Verfahren zur proteinimmobilisierung an einer festphase, so hergestellte protein tragende festphase sowie deren verwendung
DE69106002T2 (de)	1995-05-11	Testverfahren und reagenziensatz dafür.
EP0491362B1 (de)	1996-03-13	Verwendung von Peptidpaaren mit extrem hoher spezifischer Affinität zueinander im Bereich der in vitro Diagnostik
EP0408078B1 (de)	1995-11-15	Verfahren zur Herstellung einer mit einer immunologisch aktiven Substanz beschichteten Festphasenmatrix
CH650592A5 (de)	1985-07-31	Reagenz zur verwendung bei immunoassays sowie verfahren zur durchfuehrung des immunoassays.
EP0185372B1 (de)	1989-03-01	Verfahren zur Herstellung eines immunreaktiven porösen Trägermaterials
EP0782585A1 (de)	1997-07-09	RHEUMAFAKTOR-ENTSTÖRUNG MIT ANTI-Fd-ANTIKÖRPERN
EP0344578B1 (de)	1994-08-10	Verfahren zur Bestimmung einer immunologisch nachweisbaren Substanz und dazu geeignetes Reaktionsgefäss
EP0572845B1 (de)	1998-04-01	Verfahren zur immunchemischen Bestimmung eines Analyten
EP0331127B1 (de)	1993-06-16	Verfahren zur Herstellung einer Festphasenmatrix
EP0322813A2 (de)	1989-07-05	Verfahren zur Bestimmung einer immunologisch aktiven Substanz
DE3872151T2 (de)	1993-03-18	Immobilisierung einer bioaktiven substanz an einer lipidzusammensetzung, enthaltend eine modofizierte lipidverbindung.
DE19811196A1 (de)	1998-11-12	Verwendung Anti-Creatinin-Antikörpern oder anderen Creatinin bindenden Substanzen zur Bestimmung von Creatinin in physiologischen Flüssigkeiten und Verfahren zu ihrer Herstellung
EP0249877B1 (de)	1992-09-16	Verfahren zur Herstellung eines immunreaktiven Trägermaterials für die heterogene immunologische Analyse
EP1525475A2 (de)	2005-04-27	Sensoroberfl che mit verbessertem signal/rausch-verh lt nis
DE3901638C2 (de)	1999-03-25	Verfahren zur Bestimmung einer immunologisch nachweisbaren Substanz und dazu geeignetes Reaktionsgefäß