DE69918149D1 - Verbessertea verfahren zur oligonucleotid-synthese - Google Patents

Verbessertea verfahren zur oligonucleotid-synthese

Info

Publication number: DE69918149D1
Authority: DE; Germany
Prior art keywords: ppm; solvent; oligonucleotide synthesis; present; tea method
Prior art date: 1998-10-06
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

DE69918149T

Other languages

English (en)

Other versions

DE69918149T8 (de

DE69918149T2 (de

Inventor

Anthony Scozzari

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Ionis Pharmaceuticals Inc

Original Assignee

Isis Pharmaceuticals Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1998-10-06

Filing date

1999-10-01

Publication date

2004-07-22

1999-10-01 Application filed by Isis Pharmaceuticals Inc filed Critical Isis Pharmaceuticals Inc

2004-07-22 Publication of DE69918149D1 publication Critical patent/DE69918149D1/de

2005-06-09 Application granted granted Critical

2005-06-09 Publication of DE69918149T2 publication Critical patent/DE69918149T2/de

2005-09-29 Publication of DE69918149T8 publication Critical patent/DE69918149T8/de

Status Active legal-status Critical Current

Links

238000000034 method Methods 0.000 title abstract 5
230000015572 biosynthetic process Effects 0.000 title 1
238000003786 synthesis reaction Methods 0.000 title 1
WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 abstract 3
239000003153 chemical reaction reagent Substances 0.000 abstract 2
239000002777 nucleoside Substances 0.000 abstract 2
238000002515 oligonucleotide synthesis Methods 0.000 abstract 2
239000002904 solvent Substances 0.000 abstract 2
108091034117 Oligonucleotide Proteins 0.000 abstract 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
150000003833 nucleoside derivatives Chemical class 0.000 abstract 1
230000001590 oxidative effect Effects 0.000 abstract 1
150000008300 phosphoramidites Chemical class 0.000 abstract 1
239000007787 solid Substances 0.000 abstract 1
239000007790 solid phase Substances 0.000 abstract 1
230000002194 synthesizing effect Effects 0.000 abstract 1
238000005406 washing Methods 0.000 abstract 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/11—Compounds covalently bound to a solid support

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Biotechnology (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Saccharide Compounds (AREA)
Nitrogen Condensed Heterocyclic Rings (AREA)
Tea And Coffee (AREA)
Low-Molecular Organic Synthesis Reactions Using Catalysts (AREA)

DE69918149T 1998-10-06 1999-10-01 Verbessertes verfahren zur oligonucleotid-synthese Active DE69918149T8 (de)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US09/167,165 US6069243A (en)	1998-10-06	1998-10-06	Process for oligonucleotide synthesis
US167165		1998-10-06
PCT/US1999/022892 WO2000020431A1 (en)	1998-10-06	1999-10-01	Improved process for oligonucleotide synthesis

Publications (3)

Publication Number	Publication Date
DE69918149D1 true DE69918149D1 (de)	2004-07-22
DE69918149T2 DE69918149T2 (de)	2005-06-09
DE69918149T8 DE69918149T8 (de)	2005-09-29

Family

ID=22606209

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DE69918149T Active DE69918149T8 (de)	1998-10-06	1999-10-01	Verbessertes verfahren zur oligonucleotid-synthese

Country Status (6)

Country	Link
US (1)	US6069243A (de)
EP (1)	EP1119578B1 (de)
AT (1)	ATE269351T1 (de)
AU (1)	AU6409399A (de)
DE (1)	DE69918149T8 (de)
WO (1)	WO2000020431A1 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
FR2790955B1 (fr)	1999-03-19	2003-01-17	Assist Publ Hopitaux De Paris	Utilisation d'oligonucleotides stabilises comme principe actif antitumoral
US6610842B1 (en) *	1999-05-06	2003-08-26	Isis Pharmaceuticals, Inc.	Processes for the synthesis of oligomers using phosphoramidite compositions
US7135565B2 (en) *	2000-07-28	2006-11-14	Agilent Technologies, Inc.	Synthesis of polynucleotides using combined oxidation/deprotection chemistry
DE10041539A1 (de) *	2000-08-24	2002-03-07	Febit Ferrarius Biotech Gmbh	Neue Amiditderivate zur Synthese von Polymeren auf Oberflächen
US6693187B1 (en) *	2000-10-17	2004-02-17	Lievre Cornu Llc	Phosphinoamidite carboxlates and analogs thereof in the synthesis of oligonucleotides having reduced internucleotide charge
US6768005B2 (en)	2000-12-20	2004-07-27	Avecia Limited	Process
CA2714353A1 (en)	2000-12-05	2002-06-13	Avecia Biotechnology Inc	Process for the preparation of phosphorothioate oligonucleotides
WO2002061138A1 (en) *	2001-01-30	2002-08-08	Isis Pharmaceuticals, Inc.	Methods for detection of chloral hydrate in dichloroacetic acid
US6858720B2 (en)	2001-10-31	2005-02-22	Agilent Technologies, Inc.	Method of synthesizing polynucleotides using ionic liquids
US6852850B2 (en) *	2001-10-31	2005-02-08	Agilent Technologies, Inc.	Use of ionic liquids for fabrication of polynucleotide arrays
US6696298B2 (en)	2001-12-07	2004-02-24	Biosearch Technologies, Inc.	Multi-channel reagent dispensing apparatus
US7169916B2 (en) *	2002-04-01	2007-01-30	Isis Pharmaceuticals, Inc.	Chloral-free DCA in oligonucleotide synthesis
CN104736551B (zh) *	2012-08-15	2017-07-28	Ionis制药公司	使用改进的封端方案制备寡聚化合物的方法
DK2951191T3 (en) *	2013-01-31	2019-01-14	Ionis Pharmaceuticals Inc	PROCEDURE FOR MANUFACTURING OLIGOMERIC COMPOUNDS USING MODIFIED CLUTCH PROTOCOLS
EP2782332B1 (de)	2013-03-22	2017-08-30	Harvest Imaging bvba	Digitalkamera mit Fokuserkennungspixeln zur Lichtmessung
US10040048B1 (en)	2014-09-25	2018-08-07	Synthego Corporation	Automated modular system and method for production of biopolymers
US20190076814A1 (en)	2017-09-11	2019-03-14	Synthego Corporation	Biopolymer synthesis system and method
KR20230119197A (ko) *	2020-12-18	2023-08-16	일라이 릴리 앤드 캄파니	진동 유동층 올리고뉴클레오티드 합성기

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US34069A (en) *		1862-01-07		Improved combined knife, fork, and spoon
US4500707A (en) *	1980-02-29	1985-02-19	University Patents, Inc.	Nucleosides useful in the preparation of polynucleotides
US5132418A (en) *	1980-02-29	1992-07-21	University Patents, Inc.	Process for preparing polynucleotides
US4458066A (en) *	1980-02-29	1984-07-03	University Patents, Inc.	Process for preparing polynucleotides
US4668777A (en) *	1981-03-27	1987-05-26	University Patents, Inc.	Phosphoramidite nucleoside compounds
US4973679A (en) *	1981-03-27	1990-11-27	University Patents, Inc.	Process for oligonucleo tide synthesis using phosphormidite intermediates
US4415732A (en) *	1981-03-27	1983-11-15	University Patents, Inc.	Phosphoramidite compounds and processes
US4517338A (en) *	1983-06-20	1985-05-14	Chiron Corporation	Multiple reactor system and method for polynucleotide synthesis
DE3329892A1 (de) *	1983-08-18	1985-03-07	Köster, Hubert, Prof. Dr., 2000 Hamburg	Verfahren zur herstellung von oligonucleotiden
USRE34069E (en)	1983-08-18	1992-09-15	Biosyntech Gmbh	Process for the preparation of oligonucleotides
US5034506A (en) *	1985-03-15	1991-07-23	Anti-Gene Development Group	Uncharged morpholino-based polymers having achiral intersubunit linkages
US5459255A (en) *	1990-01-11	1995-10-17	Isis Pharmaceuticals, Inc.	N-2 substituted purines
US5151510A (en) *	1990-04-20	1992-09-29	Applied Biosystems, Inc.	Method of synethesizing sulfurized oligonucleotide analogs
US5292875A (en) *	1990-04-20	1994-03-08	Lynx Therapeutics, Inc.	Method of synthesizing sulfurized oligonucleotide analogs
US5218105A (en) *	1990-07-27	1993-06-08	Isis Pharmaceuticals	Polyamine conjugated oligonucleotides
US5378825A (en) *	1990-07-27	1995-01-03	Isis Pharmaceuticals, Inc.	Backbone modified oligonucleotide analogs
US5138045A (en) *	1990-07-27	1992-08-11	Isis Pharmaceuticals	Polyamine conjugated oligonucleotides
US5539082A (en) *	1993-04-26	1996-07-23	Nielsen; Peter E.	Peptide nucleic acids
US5210264A (en) *	1992-01-10	1993-05-11	Isis Pharmaceuticals, Inc.	S-(2,4-dichlorobenzyl)-β-cyanoethyl phosphorothioate diester
US5571902A (en) *	1993-07-29	1996-11-05	Isis Pharmaceuticals, Inc.	Synthesis of oligonucleotides
US5440068A (en) *	1993-12-30	1995-08-08	The Standard Oil Company	Acetonitrile purification via an adsorption-based process
US5889136A (en) *	1995-06-09	1999-03-30	The Regents Of The University Of Colorado	Orthoester protecting groups in RNA synthesis
US5902881A (en) *	1997-03-03	1999-05-11	Isis Pharmaceuticals, Inc.	Reagent useful for synthesizing sulfurized oligonucleotide analogs

1998
- 1998-10-06 US US09/167,165 patent/US6069243A/en not_active Expired - Lifetime
1999
- 1999-10-01 EP EP99951708A patent/EP1119578B1/de not_active Expired - Lifetime
- 1999-10-01 WO PCT/US1999/022892 patent/WO2000020431A1/en not_active Ceased
- 1999-10-01 DE DE69918149T patent/DE69918149T8/de active Active
- 1999-10-01 AU AU64093/99A patent/AU6409399A/en not_active Abandoned
- 1999-10-01 AT AT99951708T patent/ATE269351T1/de not_active IP Right Cessation

Also Published As

Publication number	Publication date
EP1119578A1 (de)	2001-08-01
DE69918149T8 (de)	2005-09-29
EP1119578A4 (de)	2002-04-17
WO2000020431A1 (en)	2000-04-13
ATE269351T1 (de)	2004-07-15
DE69918149T2 (de)	2005-06-09
EP1119578B1 (de)	2004-06-16
US6069243A (en)	2000-05-30
AU6409399A (en)	2000-04-26

Legal Events

Date	Code	Title	Description
2006-05-18	8364	No opposition during term of opposition

Publication	Publication Date	Title
DE69918149D1 (de)	2004-07-22	Verbessertea verfahren zur oligonucleotid-synthese
FR2540122B1 (fr)	1985-11-29	Nouveaux composes comportant une sequence d'oligonucleotide liee a un agent d'intercalation, leur procede de synthese et leur application
WO1998041531A3 (en)	1999-09-16	Solvent for biopolymer synthesis, solvent microdroplets and methods of use
EP0663447A3 (de)	1998-06-03	Verfahren für den Nachweis spezifischer Polynukleotiden
DE69940623D1 (de)	2009-04-30	Prozess zur Synthetisierung von Nukleinsäure
EP1072678A4 (de)	2006-09-27	Verfahren zum synthetisieren von dna
ATE211769T1 (de)	2002-01-15	Oligonukleotide und verfahren zum nachweis von chlamydia trachomatis
ATE277068T1 (de)	2004-10-15	2'-modifizierte oligonucleotide mit rns wirkung, die konformell eingeschränkt sind
DE69806003D1 (de)	2002-07-18	Dna polymerasen, die verbesserte fähigkeiten zum einbau markierter nukleotide besitzen
DE60025840D1 (de)	2006-04-13	Methode zum nachweis von variationen oder polymorphismen
ATE373105T1 (de)	2007-09-15	Verbindungen und methoden zum nachweis von biomolekülen
DE60044367D1 (de)	2010-06-17	Nichtaggregierende, nichtausschaltende oligomere, nukleotid-analoga enthaltend; verfahren zu deren herstellung und verwendung
ATE244259T1 (de)	2003-07-15	Verwendungen von fluoreszierenden n-nukleosiden und dessen analogen
DE69623005D1 (de)	2002-09-19	Verbesserte verfahren zur enzymatischen synthese von oligosacchariden
DE69819998D1 (de)	2004-01-08	Verfahren zur chemischen Synthese von Oligonukleotiden
ATE230034T1 (de)	2003-01-15	Verfahren zur aufreinigung von dns
EP1023310A4 (de)	2001-05-16	Synthese von sulfurierten oligonukleotiden
ATE242261T1 (de)	2003-06-15	Fluoreszend-konjugaten von nukleosiden und von nukleotiden, ihrer verfahren zur herstellung und ihrer verwendungen
ATE294186T1 (de)	2005-05-15	Verfahren zur herstellung von nukleotiden oder oligonukleotiden phosphoramitiden
PL319677A1 (en)	1998-10-26	Method of obtaining p-chiral analoques of nucleotides
EP1084266B1 (de)	2004-06-30	Anwendung von 5'-modifizierten nukleotiden in der molekularbiologie und medizin
ATE298759T1 (de)	2005-07-15	Verfahren zur herstellung von 2'-fluoro-beta-l- arabinofuranosylnukleosiden
DE69731112D1 (de)	2004-11-11	Verfahren zur synthese von phosphorothioat-oligonukelotiden
WO2000026415A3 (en)	2000-09-08	A method for genomic substractive hybridization
CA2348776A1 (en)	2000-05-11	High fidelity thermostable ligase and uses thereof