DK0396436T3 - Gærstammer til fremstilling af modne heterologe proteiner, især hirudin - Google Patents

Gærstammer til fremstilling af modne heterologe proteiner, især hirudin

Info

Publication number: DK0396436T3
Authority: DK; Denmark
Prior art keywords: production; heterologous proteins; yeast strains; hirudin; mature heterologous
Prior art date: 1989-03-31

Application number

DK90400838.0T

Other languages

English (en)

Inventor

Yves Lemoine

Martine Nguyen

Tilman Achstetter

Original Assignee

Transgene Sa

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-03-31

Filing date

1990-03-28

Publication date

1994-12-05

1990-03-28 Application filed by Transgene Sa filed Critical Transgene Sa

1994-12-05 Application granted granted Critical

1994-12-05 Publication of DK0396436T3 publication Critical patent/DK0396436T3/da

Links

240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 4
108090000623 proteins and genes Proteins 0.000 title abstract 4
102000004169 proteins and genes Human genes 0.000 title abstract 4
102000007625 Hirudins Human genes 0.000 title abstract 2
108010007267 Hirudins Proteins 0.000 title abstract 2
WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 title abstract 2
229940006607 hirudin Drugs 0.000 title abstract 2
101150045458 KEX2 gene Proteins 0.000 abstract 3
230000003321 amplification Effects 0.000 abstract 2
238000003199 nucleic acid amplification method Methods 0.000 abstract 2
101710118538 Protease Proteins 0.000 abstract 1
101100053619 Yersinia enterocolitica yscF gene Proteins 0.000 abstract 1
239000013604 expression vector Substances 0.000 abstract 1
230000037431 insertion Effects 0.000 abstract 1
238000003780 insertion Methods 0.000 abstract 1
230000010354 integration Effects 0.000 abstract 1
239000013598 vector Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Mycology (AREA)
General Health & Medical Sciences (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Medicinal Chemistry (AREA)
Biophysics (AREA)
Microbiology (AREA)
Gastroenterology & Hepatology (AREA)
Tropical Medicine & Parasitology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Peptides Or Proteins (AREA)
Coloring Foods And Improving Nutritive Qualities (AREA)

DK90400838.0T 1989-03-31 1990-03-28 Gærstammer til fremstilling af modne heterologe proteiner, især hirudin DK0396436T3 (da)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
FR8904305A FR2645174B1 (fr)	1989-03-31	1989-03-31	Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant

Publications (1)

Publication Number	Publication Date
DK0396436T3 true DK0396436T3 (da)	1994-12-05

Family

ID=9380295

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK90400838.0T DK0396436T3 (da)	1989-03-31	1990-03-28	Gærstammer til fremstilling af modne heterologe proteiner, især hirudin

Country Status (10)

Country	Link
US (1)	US5521093A (da)
EP (1)	EP0396436B1 (da)
JP (1)	JP3135550B2 (da)
AT (1)	ATE109506T1 (da)
CA (1)	CA2013239C (da)
DE (1)	DE69011183T2 (da)
DK (1)	DK0396436T3 (da)
ES (1)	ES2060974T3 (da)
FR (1)	FR2645174B1 (da)
PT (1)	PT93614B (da)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5879926A (en) *	1989-03-31	1999-03-09	Transgene S.A.	Yeast strains for the production of mature heterologous proteins, especially hirudin
CN1226282A (zh) *	1996-06-28	1999-08-18	诺沃挪第克公司	具有齿斑葡聚糖酶活性的重组酶
US6066070A (en) *	1998-04-28	2000-05-23	Toyota Jidosha Kabushiki Kaisha	Control system of vehicle having continuously variable transmission
JP3546302B2 (ja)	1999-08-05	2004-07-28	トヨタ自動車株式会社	無段変速機を備えた車両の制御装置
JP3539335B2 (ja)	2000-03-10	2004-07-07	トヨタ自動車株式会社	無段変速機を備えた車両の制御装置
EP2964666B1 (en)	2013-03-06	2020-11-18	GlaxoSmithKline LLC	Host cells and methods of use
US9388599B2 (en)	2014-02-27	2016-07-12	Parsons Corporation	Wind tower erection system
CN106337042B (zh) *	2015-07-16	2019-08-09	上海雅心生物技术有限公司	双碱基酶kex2变体及其高效表达方法
JP7163279B2 (ja)	2017-03-31	2022-10-31	日精エー・エス・ビー機械株式会社	ハンドルを備える容器
CN113913414B (zh) *	2021-05-28	2023-09-26	上海雅心生物技术有限公司	高稳定性和高催化效率的双碱基酶Kex2突变体

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS6236183A (ja) *	1985-06-20	1987-02-17	ザ・サルク・インステイチユ−ト・バイオテクノロジ−／インダストリアル・アソシエイツ・インコ−ポレ−テツド	サツカロミセス・セレビシエからのポリペプチドの発現および分泌
MC1834A1 (fr) *	1986-07-10	1988-06-03	Transgene Sa	Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation
FR2607517B2 (fr) *	1986-12-01	1989-12-22	Transgene Sa	Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu
CA1340740C (en) *	1987-12-08	1999-09-14	Eileen R. Mulvihill	Co-expression in eukaryotic cells
ES2204884T3 (es) *	1988-01-05	2004-05-01	Roche Diagnostics Gmbh	Procedimiento para la obtencion de proteinas o productos genicos que contienen proteinas.
JP2643968B2 (ja) *	1988-02-03	1997-08-25	サントリー株式会社	Ｋｅｘ２エンドプロテアーゼ及びその製造方法
AU614121B2 (en) *	1988-05-04	1991-08-22	Novartis Ag	Improvements in the production of polypeptides
FR2634495B1 (fr) *	1988-07-01	1990-10-26	Chambon Pierre	Procede de preparation d'une proteine par des levures utilisant un systeme inductible, vecteurs et souches transformees correspondantes

1989
- 1989-03-31 FR FR8904305A patent/FR2645174B1/fr not_active Expired - Fee Related
1990
- 1990-03-28 DE DE69011183T patent/DE69011183T2/de not_active Expired - Fee Related
- 1990-03-28 AT AT90400838T patent/ATE109506T1/de not_active IP Right Cessation
- 1990-03-28 ES ES90400838T patent/ES2060974T3/es not_active Expired - Lifetime
- 1990-03-28 EP EP90400838A patent/EP0396436B1/fr not_active Expired - Lifetime
- 1990-03-28 DK DK90400838.0T patent/DK0396436T3/da active
- 1990-03-28 CA CA002013239A patent/CA2013239C/fr not_active Expired - Fee Related
- 1990-03-30 PT PT93614A patent/PT93614B/pt not_active IP Right Cessation
- 1990-03-31 JP JP02087339A patent/JP3135550B2/ja not_active Expired - Fee Related
1995
- 1995-02-23 US US08/393,025 patent/US5521093A/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
EP0396436A1 (fr)	1990-11-07
US5521093A (en)	1996-05-28
ES2060974T3 (es)	1994-12-01
ATE109506T1 (de)	1994-08-15
EP0396436B1 (fr)	1994-08-03
JPH0372867A (ja)	1991-03-28
FR2645174B1 (fr)	1994-01-07
DE69011183D1 (de)	1994-09-08
JP3135550B2 (ja)	2001-02-19
FR2645174A1 (fr)	1990-10-05
PT93614A (pt)	1990-11-20
CA2013239A1 (fr)	1990-09-30
PT93614B (pt)	1996-08-30
CA2013239C (fr)	2000-01-18
DE69011183T2 (de)	1995-03-16

Publication	Publication Date	Title
BG60107B2 (bg)	1993-10-29	Метод за получаване на еукариотен полипептид
DK577783D0 (da)	1983-12-15	Vektor for udtrykkelse af et dna-segment i gaer
AU2705895A (en)	1996-01-25	Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
EP0722461A4 (en)	1997-02-26	EXPRESSION OF FUSION POLYPEPTIDES TRANSPORTED OUT OF CYTOPLASM WITHOUT HEAD SEQUENCE
ATE123520T1 (de)	1995-06-15	Kex2-endoprotease und verfahren zu ihrer herstellung.
DE69231137D1 (de)	2000-07-13	Verfahren zur Herstellung von Peroxidasen in einem Aspergillusstamm
DK0396436T3 (da)	1994-12-05	Gærstammer til fremstilling af modne heterologe proteiner, især hirudin
DE59611434D1 (de)	2007-07-26	Herstellung von Proteinen aus Pro-Proteinen durch Fusionsproteine abgeleitet von Furin oder Furinanalogen
KR880700855A (ko)	1988-04-12	진핵생물 단백질을 발현시키는 개선된 방법
PT574347E (pt)	2004-02-27	Nova protease fungica
DK0463024T3 (da)	2001-11-12	DNA-konstruktion og modificeret gær
ATE43637T1 (de)	1989-06-15	Rekombinanter mikroorganismus der art saccharomyces.
ATE202381T1 (de)	2001-07-15	Neue prosequenz zur sekretion heterologer proteine aus hefezellen
AU650038B2 (en)	1994-06-09	CKS method of protein synthesis
SE9301583D0 (sv)	1993-05-07	Yeast strain and methods for expressing heterologous proteins in yeast
KR830005348A (ko)	1983-08-13	인체 인터페론의 아미노산 서열을 가진 미생물학적으로 제조된 폴리펩타이드, 아미노산 서열, 유전정보가 함유된 미생물에 코딩으로 작용하는 dna 및 플라스미드와 이의 제조방법