DK0445646T3 - DNA-fragment, som koder for et polypeptid med nitrilhydrataseaktivitet, en transformant indeholdende DNA-fragmentet og en fremgangsmåde til fremstilling af amider under anvendelse af transformanten - Google Patents

DNA-fragment, som koder for et polypeptid med nitrilhydrataseaktivitet, en transformant indeholdende DNA-fragmentet og en fremgangsmåde til fremstilling af amider under anvendelse af transformanten

Info

Publication number
DK0445646T3
DK0445646T3 DK91102937.9T DK91102937T DK0445646T3 DK 0445646 T3 DK0445646 T3 DK 0445646T3 DK 91102937 T DK91102937 T DK 91102937T DK 0445646 T3 DK0445646 T3 DK 0445646T3
Authority
DK
Denmark
Prior art keywords
transformant
dna fragment
amides
polypeptide
prodn
Prior art date
Application number
DK91102937.9T
Other languages
Danish (da)
English (en)
Inventor
Beppu Teruhiko
Yamada Hideaki
Nagasawa Toru
Horinouchi Sueharu
Nishiyama Makoto
Original Assignee
Beppu Teruhiko
Nitto Chemical Industry Co Ltd
Yamada Hideaki
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beppu Teruhiko, Nitto Chemical Industry Co Ltd, Yamada Hideaki filed Critical Beppu Teruhiko
Application granted granted Critical
Publication of DK0445646T3 publication Critical patent/DK0445646T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK91102937.9T 1990-02-28 1991-02-27 DNA-fragment, som koder for et polypeptid med nitrilhydrataseaktivitet, en transformant indeholdende DNA-fragmentet og en fremgangsmåde til fremstilling af amider under anvendelse af transformanten DK0445646T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP4807890 1990-02-28
US08/028,463 US5731176A (en) 1990-02-28 1993-03-09 DNA fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant

Publications (1)

Publication Number Publication Date
DK0445646T3 true DK0445646T3 (da) 1996-09-30

Family

ID=26388301

Family Applications (1)

Application Number Title Priority Date Filing Date
DK91102937.9T DK0445646T3 (da) 1990-02-28 1991-02-27 DNA-fragment, som koder for et polypeptid med nitrilhydrataseaktivitet, en transformant indeholdende DNA-fragmentet og en fremgangsmåde til fremstilling af amider under anvendelse af transformanten

Country Status (18)

Country Link
US (1) US5731176A (fr)
EP (1) EP0445646B1 (fr)
JP (1) JP3162091B2 (fr)
KR (1) KR950002004B1 (fr)
CN (1) CN1054639C (fr)
AT (1) ATE138101T1 (fr)
AU (1) AU627648B2 (fr)
CA (1) CA2037291C (fr)
CZ (2) CZ284017B6 (fr)
DE (1) DE69119454T2 (fr)
DK (1) DK0445646T3 (fr)
ES (1) ES2089044T3 (fr)
FI (1) FI102539B1 (fr)
MX (1) MX193790B (fr)
NO (1) NO306684B1 (fr)
PL (1) PL166201B1 (fr)
RU (1) RU2081173C1 (fr)
SK (1) SK280199B6 (fr)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2907479B2 (ja) * 1990-02-28 1999-06-21 輝彦 別府 ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体及びアミド類の製造法
DE69231939T2 (de) * 1991-03-04 2002-04-04 Teruhiko Beppu Hybrid-Plasmid-Vektoren, die für Nitril-abbauende Enzyme kodierende Gene enthalten und Verfahren zur Herstellung von Amiden und Säuren
AU3692593A (en) * 1992-04-28 1993-11-04 Mitsubishi Kasei Corporation Novel protein having nitrile hydratase activity and the gene encoding the same, and a method for producing amides from nitriles via a transformant containing the gene
JP3828590B2 (ja) * 1994-10-03 2006-10-04 昌 清水 ニトリルヒドラターゼ遺伝子発現のための調節遺伝子
JP3154633B2 (ja) * 1994-12-28 2001-04-09 三菱レイヨン株式会社 ニトリラーゼ遺伝子発現のための調節因子およびその遺伝子
JPH11513255A (ja) * 1995-10-06 1999-11-16 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー 立体特異的ニトリルヒドラターゼおよびアミダーゼ酵素をコードする核酸断片、ならびにキラルアミドおよび酸の生産に有用なそれら酵素を発現する組換え微生物
CN1240833C (zh) * 1996-02-14 2006-02-08 三井化学株式会社 新型腈水合酶
US5866379A (en) * 1997-01-28 1999-02-02 Novus International Enzymatic conversion of α-hydroxynitriles to the corresponding .alpha.
FR2770214B1 (fr) * 1997-10-24 1999-12-31 Rhodia Chimie Sa Procede de preparation d'un benzamide halogene
JP2000050866A (ja) * 1998-08-06 2000-02-22 Rikagaku Kenkyusho ニトリルヒドラターゼの生産方法
JP4185607B2 (ja) 1998-12-15 2008-11-26 ダイセル化学工業株式会社 新規な微生物及びアミド化合物の製造方法
RU2310686C2 (ru) * 1999-06-25 2007-11-20 Басф Акциенгезелльшафт Гены corynebacterium glutamicum, кодирующие белки, участвующие в метаболизме углерода и продуцировании энергии
RU2304616C2 (ru) * 1999-06-25 2007-08-20 Басф Акциенгезелльшафт Гены corynebacterium glutamicum, кодирующие белки, участвующие в гомеостазе и адаптации
RU2312145C2 (ru) * 1999-06-25 2007-12-10 Басф Акциенгезелльшафт Гены corynebacterium glutamicum, кодирующие белки, участвующие в синтезе мембран и мембранном транспорте
RU2306339C2 (ru) * 1999-06-25 2007-09-20 Басф Акциенгезелльшафт ГЕНЫ Corynebacterium glutamicum, КОДИРУЮЩИЕ БЕЛКИ МЕТАБОЛИЧЕСКИХ ПУТЕЙ
RU2303635C2 (ru) * 1999-06-25 2007-07-27 Басф Акциенгезелльшафт Гены corynebacterium glutamicum, кодирующие белки резистентности и толерантности к стрессам
RU2326170C2 (ru) * 1999-07-01 2008-06-10 Басф Акциенгезелльшафт Гены corynebacterium glutamicum, кодирующие белки системы фосфоенолпируват-сахар-фосфотрансферазы
CN1340101A (zh) 1999-10-26 2002-03-13 昭和电工株式会社 新型红球菌属细菌、源自于红球菌属细菌的腈水解酶基因、腈水合酶基因和酰胺酶基因,以及利用它们生产羧酸的方法
DE10010149A1 (de) * 2000-03-03 2001-09-06 Basf Ag Nitrilase aus Rhodococcus rhodochrous NCIMB 11216
RU2288270C2 (ru) * 2000-12-20 2006-11-27 Диа-Нитрикс Ко., Лтд. Способ производства амидного соединения с применением микробного катализатора
CZ20031409A3 (cs) * 2001-01-09 2003-11-12 Lonza Ag Mikroorganismy a způsob výroby amidů
JP2002325587A (ja) * 2001-03-02 2002-11-12 Daicel Chem Ind Ltd ニトリルヒドラターゼ、およびアミドの製造方法
JP2004194588A (ja) 2002-12-19 2004-07-15 Mitsui Chemicals Inc 新規なニトリルヒドラターゼ
WO2004067738A2 (fr) * 2003-01-27 2004-08-12 Degussa Ag Nitrile hydratases provenant de rhodococcus erythropolis et leur application
AU2004295409B2 (en) 2003-12-02 2010-07-29 Ciba Specialty Chemicals Water Treatments Limited Strain of Rhodococcus rhodochrous NCIMB 41164 and its use as producer of nitrile hydratase
EP1767624B1 (fr) * 2004-05-26 2013-11-06 Mitsubishi Rayon Co., Ltd. Hydratase de nitrile amelioree
JP2006187257A (ja) 2005-01-07 2006-07-20 Daiyanitorikkusu Kk アミド化合物の製造方法およびアクリルアミド系ポリマー
EP1842907A1 (fr) 2006-04-07 2007-10-10 B.R.A.I.N. Ag Groupe de nouvelles nitrile hydratases microbiennes énantiosélectives à large spécifité de substrat
WO2008004473A1 (fr) * 2006-07-06 2008-01-10 University Of Tsukuba Nouveau complexe de protéines, procédé de maturation de nitrile hydratase à faible poids moléculaire de type au cobalt utilisant le complexe de protéines, nitrile hydratase correspondante et procédé l'utilisant
US8334132B2 (en) 2006-11-09 2012-12-18 Mitsubishi Rayon Co., Ltd. Process for production of a betaine such as carnitine
KR101734797B1 (ko) 2011-05-31 2017-05-24 미쯔비시 케미컬 주식회사 개량형 니트릴 히드라타제
US9193966B2 (en) 2011-06-07 2015-11-24 Mitsubishi Rayon Co., Ltd. Nitrile hydratase
FR3002542B1 (fr) * 2013-02-28 2016-01-22 Servier Lab Procede de synthese enzymatique de l'acide (7s) 3,4-dimethoxybicyclo[4.2.0]octa-1,3,5-triene 7-carboxylique et application a la synthese de l'ivabradine et de ses sels
CN112941060B (zh) 2014-06-06 2025-05-09 三菱化学株式会社 改良型腈水合酶
EP3535386A4 (fr) 2016-11-04 2020-04-15 Georgia State University Research Foundation, Inc. Asparaginase exempte d'endotoxines
EP4279600A4 (fr) 2021-02-10 2025-06-11 Mitsubishi Chemical Corporation Réactivité améliorée de nitrile hydratase à l'aide d'aldéhyde
CN114934006A (zh) * 2022-06-02 2022-08-23 无锡新晨宇生物工程有限公司 一种腈水合酶催化乙腈生成乙酰胺的应用
AU2024239889A1 (en) 2023-03-17 2025-09-18 Mitsubishi Chemical Corporation Method for producing amide compound
JPWO2024195653A1 (fr) 2023-03-17 2024-09-26

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX169933B (es) * 1987-09-18 1993-08-02 Hideaki Yamada Procedimiento para la produccion biologica de amidas
JP2840253B2 (ja) * 1988-07-06 1998-12-24 輝彦 別府 ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体によるニトリル類からアミド類の製造法

Also Published As

Publication number Publication date
JPH04211379A (ja) 1992-08-03
CZ317094A3 (cs) 1998-05-13
NO910797L (no) 1991-08-29
NO910797D0 (no) 1991-02-27
EP0445646A3 (en) 1992-01-08
CA2037291C (fr) 2001-07-24
PL166201B1 (pl) 1995-04-28
PL289239A1 (en) 1992-03-23
CA2037291A1 (fr) 1991-08-29
ATE138101T1 (de) 1996-06-15
AU7129391A (en) 1991-09-12
ES2089044T3 (es) 1996-10-01
FI102539B (fi) 1998-12-31
CZ284017B6 (cs) 1998-07-15
NO306684B1 (no) 1999-12-06
KR950002004B1 (ko) 1995-03-08
CN1054616A (zh) 1991-09-18
DE69119454D1 (de) 1996-06-20
CZ284048B6 (cs) 1998-07-15
DE69119454T2 (de) 1996-09-26
SK280199B6 (sk) 1999-09-10
CN1054639C (zh) 2000-07-19
MX193790B (en) 1999-10-19
FI910960L (fi) 1991-08-29
EP0445646A2 (fr) 1991-09-11
EP0445646B1 (fr) 1996-05-15
CS9100531A2 (en) 1991-10-15
AU627648B2 (en) 1992-08-27
JP3162091B2 (ja) 2001-04-25
US5731176A (en) 1998-03-24
KR910021473A (ko) 1991-12-20
RU2081173C1 (ru) 1997-06-10
FI102539B1 (fi) 1998-12-31
FI910960A0 (fi) 1991-02-27

Similar Documents

Publication Publication Date Title
DK0445646T3 (da) DNA-fragment, som koder for et polypeptid med nitrilhydrataseaktivitet, en transformant indeholdende DNA-fragmentet og en fremgangsmåde til fremstilling af amider under anvendelse af transformanten
NO179253C (no) Rekombinant DNA-sekvens som koder for humant proapolipoprotein A-I, ekspresjonsvektorer, cellekulturer samt fremgangsmåte for fremstilling av proteinet
DK0423302T3 (da) Anvendelse af hidtil ukendte DNA-fragmenter som kodende sekvens for et signalpeptid til sekretion af modne proteiner i rekombinant gær, ekspressionskassetter, transformeret gær og tilsvarende fremgangsmåde til fremstilling af proteiner
GB2197324B (en) Process for the production of a polypeptide
DK0786009T3 (da) Fremgangsmåde til bakteriel fremstilling af heterologe polypeptider med disulphidbindinger
ES8502476A1 (es) Un procedimiento para obtener polipeptidos similares a la hormona del crecimiento de ganado bovino
ES2092995T3 (es) Metodo para producir celulas conteniendo un adn extraño establemente integrado con un numero alto de copia, las celulas producidas por este metodo y el uso de estas celulas para producir el polipeptido codificado por el adn extraño.
DK128490D0 (da) Renset gensekvens, som koder for protein ia, ib eller begge fra neisseria gonorrhoeae, rekombinantvektorer og organismer indeholdende sekvenser, dens fremstilling og anvendelse, neisseria-stamme og dennes paavisning
DK1520860T3 (da) Rekombinant protein PilC, fremgangsmåde til dets fremstilling og anvendelse deraf
ZA916171B (en) Protein structure of the plant toxin gelonin
DK0387646T3 (da) Achromobacter-protease I-gen og genprodukt deraf
IL94422A0 (en) Saf polypeptide,gene coding therefor,saf promoter and expression methods for expressing foreign dna sequences in streptomyces