DK0492964T3 - Kloning og ekspression af Borrelialipoproteiner - Google Patents

Kloning og ekspression af Borrelialipoproteiner

Info

Publication number: DK0492964T3
Authority: DK; Denmark
Prior art keywords: ospa; pet9; cloning; protein; purifying
Prior art date: 1990-12-21

Application number

DK91311758T

Other languages

English (en)

Inventor

John J Dunn

Alan G Barbour

Original Assignee

Brookhaven Science Ass Llc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-12-21

Filing date

1991-12-18

Publication date

2001-10-08

1991-12-18 Application filed by Brookhaven Science Ass Llc filed Critical Brookhaven Science Ass Llc

2001-10-08 Application granted granted Critical

2001-10-08 Publication of DK0492964T3 publication Critical patent/DK0492964T3/da

Links

102000004895 Lipoproteins Human genes 0.000 title abstract 2
108090001030 Lipoproteins Proteins 0.000 title abstract 2
238000010367 cloning Methods 0.000 title abstract 2
101710105714 Outer surface protein A Proteins 0.000 abstract 3
101900341263 Borrelia burgdorferi Outer surface protein A Proteins 0.000 abstract 2
230000003321 amplification Effects 0.000 abstract 2
239000013604 expression vector Substances 0.000 abstract 2
238000000034 method Methods 0.000 abstract 2
238000003199 nucleic acid amplification method Methods 0.000 abstract 2
241000589968 Borrelia Species 0.000 abstract 1
241000588724 Escherichia coli Species 0.000 abstract 1
208000016604 Lyme disease Diseases 0.000 abstract 1
108091034117 Oligonucleotide Proteins 0.000 abstract 1
108090000233 Signal peptidase II Proteins 0.000 abstract 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
239000013612 plasmid Substances 0.000 abstract 1
238000003752 polymerase chain reaction Methods 0.000 abstract 1
108090000623 proteins and genes Proteins 0.000 abstract 1
102000004169 proteins and genes Human genes 0.000 abstract 1
230000002194 synthesizing effect Effects 0.000 abstract 1
230000001131 transforming effect Effects 0.000 abstract 1
230000014616 translation Effects 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/20—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Spirochaetales (O), e.g. Treponema, Leptospira
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Molecular Biology (AREA)
Wood Science & Technology (AREA)
General Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Medicinal Chemistry (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Microbiology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Gastroenterology & Hepatology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Compounds Of Unknown Constitution (AREA)
Saccharide Compounds (AREA)
Steroid Compounds (AREA)
External Artificial Organs (AREA)
Polysaccharides And Polysaccharide Derivatives (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

DK91311758T 1990-12-21 1991-12-18 Kloning og ekspression af Borrelialipoproteiner DK0492964T3 (da)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US63207290A	1990-12-21	1990-12-21

Publications (1)

Publication Number	Publication Date
DK0492964T3 true DK0492964T3 (da)	2001-10-08

Family

ID=24533958

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK91311758T DK0492964T3 (da)	1990-12-21	1991-12-18	Kloning og ekspression af Borrelialipoproteiner

Country Status (14)

Country	Link
US (1)	US5571718A (da)
EP (1)	EP0492964B1 (da)
JP (1)	JP2706397B2 (da)
AT (1)	ATE204020T1 (da)
AU (1)	AU656159B2 (da)
CA (1)	CA2057536C (da)
DE (1)	DE69132681T2 (da)
DK (1)	DK0492964T3 (da)
FI (1)	FI105924B (da)
IE (1)	IE66473B1 (da)
IL (1)	IL100422A0 (da)
NO (1)	NO303453B1 (da)
PT (1)	PT99918B (da)
ZA (1)	ZA919965B (da)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE4015911A1 (de)	1989-09-19	1991-03-28	Max Planck Gesellschaft	Impfstoff gegen die lyme-krankheit
SG122738A1 (en)	1990-06-15	2006-06-29	Univ Yale	Compositions and methods for the prevention and diagnosis of lyme disease
US6113914A (en) *	1991-08-15	2000-09-05	Smithkline Beecham Biologicals (S.A.)	Osp A proteins of Borrelia burgdorferi subgroups, encoding genes and vaccines
US6676942B1 (en) *	1991-08-15	2004-01-13	Smithkline Beecham Biologicals (S.A.)	Osp a proteins of Borrelia burgdorferi subgroups, encoding genes and vaccines
CA2121121C (en) *	1991-10-18	2003-06-10	Lorne F. Erdile	Preparation of recombinant borrelia proteins
BE1006728A3 (fr) *	1993-02-19	1994-11-29	Wallone Region	Sondes d'acides nucleiques specifiques au spirochete borrelia burgdorferi.
US5439808A (en)	1993-07-23	1995-08-08	North American Vaccine, Inc.	Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis
US6004534A (en) *	1993-07-23	1999-12-21	Massachusetts Institute Of Technology	Targeted polymerized liposomes for improved drug delivery
US7008625B2 (en)	1993-11-01	2006-03-07	Research Foundation Of The State University Of New York	Recombinant constructs of Borrelia burgdorferi
US6248562B1 (en)	1993-11-01	2001-06-19	Research Foundation State University Of New York	Chimeric proteins comprising borrelia polypeptides and uses therefor
US5747287A (en) *	1995-04-28	1998-05-05	North American Vaccine, Inc.	Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis
DE69733944T3 (de) *	1996-02-21	2012-07-26	The Board Of Regents, The University Of Texas System	Vmp-ähnliche sequenzen von pathogener borrelia
US5846946A (en) *	1996-06-14	1998-12-08	Pasteur Merieux Serums Et Vaccins	Compositions and methods for administering Borrelia DNA
AU722630B2 (en) *	1997-02-14	2000-08-10	Higeta Shoyu Co., Ltd.	Modified Treponema pallidum-derived antigen protein
US6060082A (en) *	1997-04-18	2000-05-09	Massachusetts Institute Of Technology	Polymerized liposomes targeted to M cells and useful for oral or mucosal drug delivery
US6689573B1 (en) *	1999-05-24	2004-02-10	New England Biolabs, Inc.	Method for screening restriction endonucleases
EP1311540B1 (en) *	2000-08-18	2007-12-19	Research Foundation Of State University Of New York	Altered ospa of borrelia burgdorferi
US7729322B2 (en) *	2002-02-28	2010-06-01	Qualcomm Incorporated	HDLC hardware accelerator
AU2003299872A1 (en)	2002-12-20	2004-07-22	Board Of Regents, The University Of Texas System	Vmp-like sequences of pathogenic borrelia species and strains
WO2009135118A2 (en) *	2008-05-02	2009-11-05	Virginia Commonwealth University	Lyme disease vaccine
CN112584858A (zh) *	2018-04-03	2021-03-30	赛诺菲	抗原性ospa多肽
US12631635B2 (en) *	2021-01-15	2026-05-19	Id-Fish Technology, Inc.	Species-specific antigen sequences for tick-borne relapsing fever (TBRF) and methods of use

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DK590288D0 (da) *	1988-10-24	1988-10-24	Symbicom Ab	Kemiske forbindelser
DE4015911A1 (de) *	1989-09-19	1991-03-28	Max Planck Gesellschaft	Impfstoff gegen die lyme-krankheit
SG122738A1 (en) *	1990-06-15	2006-06-29	Univ Yale	Compositions and methods for the prevention and diagnosis of lyme disease
IE912303A1 (en) *	1990-07-06	1992-01-15	American Home Prod	Vaccine against lyme disease and a challenge model for¹evaluating vaccine efficiency

1991
- 1991-12-12 CA CA002057536A patent/CA2057536C/en not_active Expired - Fee Related
- 1991-12-18 ZA ZA919965A patent/ZA919965B/xx unknown
- 1991-12-18 DK DK91311758T patent/DK0492964T3/da active
- 1991-12-18 AU AU89834/91A patent/AU656159B2/en not_active Ceased
- 1991-12-18 DE DE69132681T patent/DE69132681T2/de not_active Expired - Lifetime
- 1991-12-18 AT AT91311758T patent/ATE204020T1/de not_active IP Right Cessation
- 1991-12-18 EP EP91311758A patent/EP0492964B1/en not_active Expired - Lifetime
- 1991-12-19 IL IL100422A patent/IL100422A0/xx unknown
- 1991-12-19 IE IE444991A patent/IE66473B1/en not_active IP Right Cessation
- 1991-12-19 FI FI916023A patent/FI105924B/fi not_active IP Right Cessation
- 1991-12-20 NO NO915051A patent/NO303453B1/no unknown
- 1991-12-20 PT PT99918A patent/PT99918B/pt not_active IP Right Cessation
- 1991-12-21 JP JP3361129A patent/JP2706397B2/ja not_active Expired - Fee Related
1992
- 1992-09-08 US US07/941,523 patent/US5571718A/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
CA2057536C (en)	1999-10-26
IE66473B1 (en)	1995-12-27
AU8983491A (en)	1992-06-25
FI105924B (fi)	2000-10-31
JP2706397B2 (ja)	1998-01-28
EP0492964A2 (en)	1992-07-01
DE69132681D1 (de)	2001-09-13
IL100422A0 (en)	1992-09-06
JPH0641195A (ja)	1994-02-15
PT99918A (pt)	1992-12-31
EP0492964A3 (en)	1993-02-24
FI916023A0 (fi)	1991-12-19
DE69132681T2 (de)	2001-11-22
IE914449A1 (en)	1992-07-01
NO915051L (no)	1992-06-22
AU656159B2 (en)	1995-01-27
NO915051D0 (no)	1991-12-20
PT99918B (pt)	1999-08-31
ZA919965B (en)	1992-11-25
US5571718A (en)	1996-11-05
FI916023L (fi)	1992-06-22
CA2057536A1 (en)	1992-06-22
NO303453B1 (no)	1998-07-13
EP0492964B1 (en)	2001-08-08
ATE204020T1 (de)	2001-08-15

Publication	Publication Date	Title
NO915051L (no)	1992-06-22	Kloning og ekspresjon av borrelie lipoproteiner
DK0792363T3 (da)	2004-04-26	Nye pesticide proteiner og stammer
NO961115D0 (no)	1996-03-19	Humane metabotrope glutamatreseptorsubtyper (HMR4, HMR6, HMR7) og relaterte DNA forbindelser
DK0652960T3 (da)	2001-04-17	Cyklisk GMP-bindende, cyklisk GMP-specifikke phosphodiesterasematerialer og fremgangsmåder
FR2637612B1 (fr)	1993-09-10	Sequences de nucleotides codant pour une proteine a activite ureasique
DE69433690D1 (de)	2004-05-13	Chimere proteine, welche borrelia polypeptide beinhalten, verwendungen dafür
EP0205475A4 (en)	1990-06-27	RECOMBINANT METHOD FOR THE PRODUCTION OF SERINE PROTEASE INHIBITORS, AND DNA SEQUENCES THEREOF.
NO180239C (no)	1997-03-12	Ekspresjonsvektor som koder for, og kan uttrykke et polypeptid med de biologiske og immunologiske egenskapene til EqIFN-<gamma>, vertsorganisme transformert med denne, og fremgangsmåte for fremstilling av nevnte polypeptid
DK0476233T3 (da)	1996-03-11	Humant MK gen og proteinsekvens
IS4609A (is)	1997-11-07	Stýrlar til að tjá gen
NO971139L (no)	1997-03-12	Fremgangsmåte for fremstilling av rekombinante proteiner, plasmider og modifiserte celler
DK563082A (da)	1982-12-20	Fremgangsmaade og vektororganisme til styret akkumulation af klonerede heterologe genprodukter i bacillus subtilis ii
EP2172214A3 (en)	2011-03-16	Chlamydia antigens and corresponding DNA fragments and uses thereof
DK422786A (da)	1986-12-05	Rekombinant-dna-sekvens til styring af den intracellulaere produktionaf metalloproteinase-inhibitorer og fremgangsmaade til fremstillingaf en saadan sekvens
ES2170082T3 (es)	2002-08-01	Transactivador de mhc clase ii (ciita) y sus usos.
DK612889A (da)	1990-06-08	Enzym og fremgangsmaade til fremstilling deraf
DK595187D0 (da)	1987-11-13	Minactivin og fremgangsmaade til fremstilling deraf
DK1241186T4 (da)	2013-02-11	Humant vækststandsende specifikt gen-6 (gas-6) og nukleinsyrer kodende derfor
AU6999287A (en)	1987-09-17	Insecticidal proteinaceous substance
ES8506800A1 (es)	1985-08-01	Un metodo de fabricar un vector de plasmido recombinante deseado.
KR970702920A (ko)	1997-06-10	시스-3-히드록시-엘-프롤린의 제조법(process for producing cis-3- hydroxy-l-proline)
IT1251680B (it)	1995-05-19	Clonaggio e sequenziamento dell'operone srfa di bacillus subtilis che codifica il complesso multienzimatico surfattina sintetasi