DK0645449T3 - Fremgangsmåde til specifik kloning af nulcleinsyrer - Google Patents

Fremgangsmåde til specifik kloning af nulcleinsyrer

Info

Publication number
DK0645449T3
DK0645449T3 DK94114675T DK94114675T DK0645449T3 DK 0645449 T3 DK0645449 T3 DK 0645449T3 DK 94114675 T DK94114675 T DK 94114675T DK 94114675 T DK94114675 T DK 94114675T DK 0645449 T3 DK0645449 T3 DK 0645449T3
Authority
DK
Denmark
Prior art keywords
nucleic acids
specific cloning
ligated
cloning
strand
Prior art date
Application number
DK94114675T
Other languages
English (en)
Inventor
Wolf Dr Bertling
Original Assignee
November Ag Molekulare Medizin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by November Ag Molekulare Medizin filed Critical November Ag Molekulare Medizin
Application granted granted Critical
Publication of DK0645449T3 publication Critical patent/DK0645449T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK94114675T 1993-09-24 1994-09-17 Fremgangsmåde til specifik kloning af nulcleinsyrer DK0645449T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4332463A DE4332463A1 (de) 1993-09-24 1993-09-24 Verfahren zur spezifischen Klonierung von Nukleinsäuren

Publications (1)

Publication Number Publication Date
DK0645449T3 true DK0645449T3 (da) 2002-05-21

Family

ID=6498466

Family Applications (1)

Application Number Title Priority Date Filing Date
DK94114675T DK0645449T3 (da) 1993-09-24 1994-09-17 Fremgangsmåde til specifik kloning af nulcleinsyrer

Country Status (6)

Country Link
EP (1) EP0645449B1 (da)
JP (1) JPH07177885A (da)
AT (1) ATE214096T1 (da)
DE (2) DE4332463A1 (da)
DK (1) DK0645449T3 (da)
ES (1) ES2173897T3 (da)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6033851A (en) * 1994-12-09 2000-03-07 Wakunaga Seiyaku Kabushiki Kaisha Method for suppressing nonspecific hybridization in primer extension method
US5753462A (en) * 1995-06-07 1998-05-19 Zymogenetics, Inc. Secretion leader trap cloning method
EP0832209A1 (en) * 1995-06-07 1998-04-01 ZymoGenetics, Inc. Secretion leader trap cloning method
DE19601385A1 (de) * 1996-01-16 1997-07-17 Mueller Manfred W Verfahren zur Amplifikation von Nukleinsäure
EP0981535A4 (en) * 1997-05-12 2000-11-29 Life Technologies Inc Methods for production and purification of nucleic acid molecules
US6399334B1 (en) 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
US6368834B1 (en) 1999-04-06 2002-04-09 Genome Technologies, Llc PCR genome walking with synthetic primer
US6083727A (en) * 1999-06-30 2000-07-04 Incyte Pharmaceuticals Inc. Methods and compositions for producing 5' enriched cDNA libraries
JP4340779B2 (ja) 2000-10-05 2009-10-07 独立行政法人理化学研究所 可変突出部位を含むオリゴヌクレオチドリンカー及び前記リンカーを用いたポリヌクレオチドライブラリーの調製方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4666839A (en) * 1982-12-01 1987-05-19 Amgen Methods and materials for obtaining microbial expression of polypeptides including bovine prolactin
DE3929030A1 (de) * 1989-09-01 1991-03-07 Boehringer Mannheim Gmbh Verfahren zur vermehrung von nukleinsaeuren
GB9011454D0 (en) * 1990-05-22 1990-07-11 Medical Res Council Polynucleotide amplification
FR2678639B1 (fr) * 1991-07-03 1993-09-17 Rhone Poulenc Rorer Sa Procede de clonage d'acides nucleiques.

Also Published As

Publication number Publication date
EP0645449B1 (de) 2002-03-06
ATE214096T1 (de) 2002-03-15
EP0645449A1 (de) 1995-03-29
ES2173897T3 (es) 2002-11-01
DE59410068D1 (de) 2002-04-11
JPH07177885A (ja) 1995-07-18
DE4332463A1 (de) 1995-03-30

Similar Documents

Publication Publication Date Title
DE69837913D1 (de) Verfahren zur vervielfältigung von nukleinsäure
EP0663447A3 (en) Method of detecting a specific polynucleotide
WO1996023079A3 (en) Method for suppressing dna fragment amplification during pcr
WO2003033724A3 (en) Nucleic acid amplification
WO1997041260A3 (en) Subtractive amplification
DK0549107T3 (da) Fremgangsmåde til fremstilling af et polynukleotidtil anvendelse ved enkeltprimeramplifikation og phosphorthioatholdige oli
ATE269908T1 (de) Methode zur sequenzierung von nukleinsäuren
ATE239090T1 (de) Methode zum generieren von einzelsträngigen dna molekülen
DE69627698D1 (de) Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
ATE205543T1 (de) Verfahren zur verbesserung der nukleinsäureamplifizierung
KR920014937A (ko) 사슬 치환에 의한 증폭방법
WO2001020035A3 (en) Methods and compositions for linear isothermal amplification of polynucleotide sequences
DK0676476T3 (da) Isotermisk nukleinsyreamplificering ved strengdeplacering
DE69736667D1 (de) Verfahren zum nachweis und amplifikation von nukleinsäuresequenzen unter verbrauch von modifizierten oligonukleotiden mit erhöhter zielschmelztemperatur (tm)
DE50206993D1 (de) Verfahren zur hemmung der expression eine zielgens
DK0721988T3 (da) Kimærisk oligonucleotid og dets anvendelse ved opnåelse af transkripter af en nucleinsyre
CA2476564A1 (en) Polynomial amplification of nucleic acids
CA2344741A1 (en) Multi-loci genomic analysis
EP1212449A4 (en) AMPLIFICATION OF FLANKING SEQUENCES FROM UNKNOWN DNA
EP1111044A4 (en) METHOD OF DNA SYNTHESIS
DK0645449T3 (da) Fremgangsmåde til specifik kloning af nulcleinsyrer
WO2003102232A3 (de) Vermehrung von ribonukleinsäuren
NO20000538L (no) Forsterkning av spesifisiteten til nukleinsyreamplifisering ved bærer nukleinsyre
ATE203775T1 (de) Methode zur detektion einer gezielten nukleinsäure
DE602008004702D1 (de) Verfahren zur Amplifikation von Nukleinsäure