ATE269908T1 - Methode zur sequenzierung von nukleinsäuren - Google Patents

Methode zur sequenzierung von nukleinsäuren

Info

Publication number
ATE269908T1
ATE269908T1 AT98913953T AT98913953T ATE269908T1 AT E269908 T1 ATE269908 T1 AT E269908T1 AT 98913953 T AT98913953 T AT 98913953T AT 98913953 T AT98913953 T AT 98913953T AT E269908 T1 ATE269908 T1 AT E269908T1
Authority
AT
Austria
Prior art keywords
nucleic acid
nucleic acids
sequencing nucleic
primers
nucleotide
Prior art date
Application number
AT98913953T
Other languages
English (en)
Inventor
Eric H Kawashima
Laurent Farinelli
Pascal Mayer
Original Assignee
Manteia S A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9706529.6A external-priority patent/GB9706529D0/en
Priority claimed from GBGB9713236.9A external-priority patent/GB9713236D0/en
Application filed by Manteia S A filed Critical Manteia S A
Application granted granted Critical
Publication of ATE269908T1 publication Critical patent/ATE269908T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AT98913953T 1997-04-01 1998-04-01 Methode zur sequenzierung von nukleinsäuren ATE269908T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9706529.6A GB9706529D0 (en) 1997-04-01 1997-04-01 Invention
GBGB9713236.9A GB9713236D0 (en) 1997-06-23 1997-06-23 Invention
PCT/GB1998/000963 WO1998044152A1 (en) 1997-04-01 1998-04-01 Method of nucleic acid sequencing

Publications (1)

Publication Number Publication Date
ATE269908T1 true ATE269908T1 (de) 2004-07-15

Family

ID=26311291

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98913953T ATE269908T1 (de) 1997-04-01 1998-04-01 Methode zur sequenzierung von nukleinsäuren

Country Status (7)

Country Link
US (1) US20020055100A1 (de)
EP (2) EP1498494A3 (de)
JP (1) JP2001517948A (de)
AT (1) ATE269908T1 (de)
AU (1) AU6846798A (de)
DE (1) DE69824716D1 (de)
WO (1) WO1998044152A1 (de)

Families Citing this family (332)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1591541B1 (de) 1997-04-01 2012-02-15 Illumina Cambridge Limited Verfahren zur Vervielfältigung von Nukleinsäuren
CA2305449A1 (en) 1997-10-10 1999-04-22 President & Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
JP4638043B2 (ja) 1998-12-14 2011-02-23 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド ポリメラーゼ合成による、単一分子の核酸の配列決定のためのシステムおよび方法
EP1165839A2 (de) * 1999-03-26 2002-01-02 Whitehead Institute For Biomedical Research Universal arrays
CA2366374C (en) 1999-04-09 2009-09-22 Keygene N.V. Method for the detection and/or analysis, by means of primer extension techniques, of single nucleotide polymorphisms in restriction fragments, in particular in amplified restriction fragments generated using aflp
WO2000065098A2 (en) * 1999-04-27 2000-11-02 University Of Chicago Nucleotide extension on a microarray of gel-immobilized primers
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
WO2001021838A2 (en) * 1999-09-22 2001-03-29 Motorola Inc. Three-dimensional microarray system for parallel genotyping of single nucleotide polymorphisms
AU7751600A (en) 1999-10-06 2001-05-10 Amersham Biosciences Corp. Method for detecting mutations using arrayed primer extension
JP2003516129A (ja) * 1999-11-04 2003-05-13 カリフォルニア・インスティテュート・オブ・テクノロジー ポリヌクレオチド配列を解析する方法および装置
IL138229A (en) * 2000-09-04 2005-03-20 Yissum Res Dev Co Method and system for detecting nucleic acids
US8039602B2 (en) * 2000-10-31 2011-10-18 Ryogen Llc Isolated genomic polynucleotide fragments from the p15 region of chromosome 11
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
EP1423529B1 (de) * 2001-01-24 2013-11-20 Genomic Expression APS Verfahren zur analyse der genexpression
WO2002072892A1 (en) 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
DK1370690T3 (da) 2001-03-16 2012-07-09 Kalim Mir Arrays og fremgangsmåder til anvendelse heraf
AU2003214765B2 (en) * 2002-04-04 2007-07-26 Qiagen Gmbh New method
AU2003265185A1 (en) * 2002-09-27 2004-04-19 Biotage Ab New sequencing method for sequencing rna molecules
CA2513889A1 (en) 2003-01-29 2004-08-19 454 Corporation Double ended sequencing
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
CA2897376A1 (en) * 2003-02-26 2004-09-10 Radoje T. Drmanac Modular system and probes for dna analysis
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20110059865A1 (en) 2004-01-07 2011-03-10 Mark Edward Brennan Smith Modified Molecular Arrays
EP2248911A1 (de) 2004-02-19 2010-11-10 Helicos Biosciences Corporation Verfahren und Zusammensetzungen zur Analyse von Polynukleotidsequenzen
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
CA2566806A1 (en) 2004-05-25 2006-01-19 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US20060062531A1 (en) 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
AU2006259565B2 (en) 2005-06-15 2011-01-06 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
AT502549B1 (de) 2005-10-07 2007-06-15 Anagnostics Bioanalysis Gmbh Vorrichtung zur analyse von flüssigen proben
WO2007133831A2 (en) * 2006-02-24 2007-11-22 Callida Genomics, Inc. High throughput genome sequencing on dna arrays
US7960104B2 (en) * 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
US20070168197A1 (en) * 2006-01-18 2007-07-19 Nokia Corporation Audio coding
EP1987159B2 (de) 2006-02-08 2020-08-12 Illumina Cambridge Limited Verfahren zur sequenzierung einer polynukleotidmatrize
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
EP2021503A1 (de) * 2006-03-17 2009-02-11 Solexa Ltd. Isothermische methoden zur entstehung von arrays von einzelnen molekülen
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20080221832A1 (en) * 2006-11-09 2008-09-11 Complete Genomics, Inc. Methods for computing positional base probabilities using experminentals base value distributions
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
WO2008157640A2 (en) * 2007-06-18 2008-12-24 Illumina, Inc. Microfabrication methods for the optimal patterning of substrates
US20090093378A1 (en) * 2007-08-29 2009-04-09 Helen Bignell Method for sequencing a polynucleotide template
WO2009052214A2 (en) * 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8298768B2 (en) 2007-11-29 2012-10-30 Complete Genomics, Inc. Efficient shotgun sequencing methods
US7897344B2 (en) * 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
US7901890B2 (en) 2007-11-05 2011-03-08 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
US8617811B2 (en) 2008-01-28 2013-12-31 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
WO2009094583A1 (en) * 2008-01-23 2009-07-30 Complete Genomics, Inc. Methods and compositions for preventing bias in amplification and sequencing reactions
US12060554B2 (en) 2008-03-10 2024-08-13 Illumina, Inc. Method for selecting and amplifying polynucleotides
EP3269824A1 (de) 2008-03-28 2018-01-17 Pacific Biosciences Of California, Inc. Zusammensetzungen und verfahren zur nukleinsäuresequenzierung
WO2009132028A1 (en) * 2008-04-21 2009-10-29 Complete Genomics, Inc. Array structures for nucleic acid detection
US20100047876A1 (en) * 2008-08-08 2010-02-25 President And Fellows Of Harvard College Hierarchical assembly of polynucleotides
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
CA2738578C (en) 2008-09-23 2021-05-04 Quantalife, Inc. Droplet-based assay system
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
EP2607496B1 (de) 2008-12-23 2014-07-16 Illumina, Inc. Für Nukleinsäuresequenzierungsprotokolle geeignete Verfahren
WO2010117804A2 (en) * 2009-03-30 2010-10-14 The Regents Of The University Of California Mostly natural dna sequencing by synthesis
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
ES2595055T3 (es) 2009-08-25 2016-12-27 Illumina, Inc. Métodos para seleccionar y amplificar polinucleótidos
CA2767056C (en) 2009-09-02 2018-12-04 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
US10072287B2 (en) 2009-09-10 2018-09-11 Centrillion Technology Holdings Corporation Methods of targeted sequencing
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
US8524450B2 (en) 2009-10-30 2013-09-03 Illumina, Inc. Microvessels, microparticles, and methods of manufacturing and using the same
EP2539464B1 (de) 2010-02-23 2016-11-16 Illumina, Inc. Verfahren zur minimierung sequenzabhängiger amplifikationsunterschiede
DE202011003570U1 (de) 2010-03-06 2012-01-30 Illumina, Inc. Systeme und Vorrichtungen zum Detektieren optischer Signale aus einer Probe
EP2556170A4 (de) 2010-03-25 2014-01-01 Quantalife Inc Tröpfchentransport- und erkennungssystem
CA2767182C (en) 2010-03-25 2020-03-24 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
AU2011237729B2 (en) 2010-04-05 2014-04-03 Prognosys Biosciences, Inc. Spatially encoded biological assays
US9353412B2 (en) 2010-06-18 2016-05-31 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
EP2632593B1 (de) 2010-10-27 2021-09-29 Illumina, Inc. Durchflusszellen zur biologischen oder chemischen analyse
SG190074A1 (en) 2010-11-01 2013-06-28 Bio Rad Laboratories System for forming emulsions
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
WO2012106546A2 (en) 2011-02-02 2012-08-09 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
AU2012231098B2 (en) 2011-03-18 2016-09-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2012149042A2 (en) 2011-04-25 2012-11-01 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
KR102003660B1 (ko) 2011-07-13 2019-07-24 더 멀티플 마이얼로머 리서치 파운데이션, 인크. 데이터 수집 및 분배 방법
EP3623481B1 (de) 2011-09-23 2021-08-25 Illumina, Inc. Zusammensetzungen zur nukleinsäuresequenzierung
CA3003082C (en) 2011-10-28 2020-12-15 Illumina, Inc. Microarray fabrication system and method
WO2013102090A1 (en) * 2011-12-28 2013-07-04 Ibis Biosciences, Inc. Isothermal amplification systems and methods
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
CA3138752C (en) 2012-04-03 2024-02-06 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
WO2013155531A2 (en) 2012-04-13 2013-10-17 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
EP2662692A1 (de) 2012-05-11 2013-11-13 Alacris Theranostics GmbH Elektrochemische Erkennung von Polymerasereaktionen durch spezifische Metallphosphatkomplexbildung
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
CA3040684C (en) 2012-08-20 2023-02-07 Hod Finkelstein Method and system for fluorescence lifetime based sequencing
US9593365B2 (en) 2012-10-17 2017-03-14 Spatial Transcriptions Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
EP2746405B1 (de) 2012-12-23 2015-11-04 HS Diagnomics GmbH Verfahren und Primersätze zur PCR-Hochdurchsatzsequenzierung
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
US20160023208A1 (en) 2013-03-13 2016-01-28 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
EP3553175B1 (de) 2013-03-13 2021-07-14 Illumina, Inc. Verfahren zur herstellung von nukleinsäuresequenzierungsbibliotheken
EP2971071B1 (de) 2013-03-15 2018-02-28 Illumina, Inc. Mit enzymen verknüpfte nukleotide
DK3013983T3 (da) 2013-06-25 2023-03-06 Prognosys Biosciences Inc Spatialt kodede biologiske assays ved brug af en mikrofluidisk anordning
MX363806B (es) 2013-07-01 2019-04-03 Illumina Inc Funcionalización de superficies sin catalizador e injerto de polímeros..
EP3241913B1 (de) 2013-07-03 2019-02-20 Illumina, Inc. System zur sequenzierung durch orthogonale synthese
FI3030645T3 (fi) 2013-08-08 2023-02-10 Fluidijärjestelmä reagenssien toimittamiseen virtauskennoon
MX371428B (es) 2013-08-19 2020-01-30 Singular Bio Inc Ensayos para la deteccion de una sola molecula y uso de los mismos.
JP2016539343A (ja) 2013-08-30 2016-12-15 イルミナ インコーポレイテッド 親水性または斑状親水性表面上の液滴の操作
EP3080268A1 (de) 2013-12-09 2016-10-19 Illumina, Inc. Verfahren und zusammensetzungen zur gezielten nukleinsäuresequenzierung
CN110411998B (zh) 2013-12-10 2022-06-07 伊鲁米那股份有限公司 用于生物或化学分析的生物传感器及其制造方法
WO2015095291A1 (en) 2013-12-19 2015-06-25 Illumina, Inc. Substrates comprising nano-patterning surfaces and methods of preparing thereof
US10246746B2 (en) 2013-12-20 2019-04-02 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic DNA samples
US10537889B2 (en) * 2013-12-31 2020-01-21 Illumina, Inc. Addressable flow cell using patterned electrodes
ES2876432T3 (es) 2014-05-16 2021-11-12 Illumina Inc Técnicas de síntesis de ácidos nucleicos
CA2949984C (en) 2014-05-27 2021-10-19 Illumina, Inc. Systems and methods for biochemical analysis including a base instrument and a removable cartridge
LT3669985T (lt) 2014-06-05 2022-06-10 Illumina, Inc. Sistemos, įskaitant sukamąjį vožtuvą, skirtos bent vieno mėginio paruošimui arba mėginio analizei
WO2015189636A1 (en) 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
WO2016003814A1 (en) 2014-06-30 2016-01-07 Illumina, Inc. Methods and compositions using one-sided transposition
EP3183577B1 (de) 2014-08-21 2020-08-19 Illumina Cambridge Limited Reversible oberflächenfunktionalisierung
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
JP6802154B2 (ja) 2014-09-18 2020-12-16 イラミーナ インコーポレーテッド 核酸シーケンシングデータを解析するための方法およびシステム
KR20170066540A (ko) 2014-10-09 2017-06-14 일루미나, 인코포레이티드 액체 중 적어도 하나를 효과적으로 격리시키기 위해 비혼화성 액체를 분리하기 위한 방법 및 디바이스
JP6808617B2 (ja) 2014-10-17 2021-01-06 イルミナ ケンブリッジ リミテッド 連続性を維持した転位
CN114805710B (zh) 2014-10-31 2023-11-17 伊鲁米纳剑桥有限公司 聚合物以及dna共聚物涂层
AU2016219943A1 (en) 2015-02-18 2017-10-12 Singular Bio, Inc. Assays for single molecule detection and use thereof
US9976174B2 (en) 2015-03-24 2018-05-22 Illumina Cambridge Limited Methods, carrier assemblies, and systems for imaging samples for biological or chemical analysis
CN120425026A (zh) 2015-04-10 2025-08-05 十程基因技术瑞典公司 生物样本的空间区别、多重核酸分析
JP2018518157A (ja) 2015-05-11 2018-07-12 イラミーナ インコーポレーテッド 治療薬剤の発見および分析のためのプラットフォーム
KR102134953B1 (ko) 2015-05-29 2020-07-16 일루미나, 인코포레이티드 지정된 반응을 수행하기 위한 샘플 캐리어 및 검정 시스템
CN107924121B (zh) 2015-07-07 2021-06-08 亿明达股份有限公司 经由纳米压印的选择性表面图案化
WO2017015018A1 (en) 2015-07-17 2017-01-26 Illumina, Inc. Polymer sheets for sequencing applications
AU2016298541B2 (en) 2015-07-30 2019-10-31 Illumina, Inc. Orthogonal deblocking of nucleotides
CN108474805A (zh) 2015-08-24 2018-08-31 亿明达股份有限公司 用于生物和化学测定的线路内蓄压器和流量控制系统
CN107921432A (zh) 2015-09-02 2018-04-17 伊卢米纳剑桥有限公司 改善流控系统中的液滴操作的系统和方法
WO2017042040A1 (en) 2015-09-09 2017-03-16 Qiagen Gmbh Polymerase enzyme
DE102015011970B4 (de) 2015-09-09 2017-04-06 Scienion Ag Dispensiergerät und entsprechendes Dispensierverfahren
US10253352B2 (en) 2015-11-17 2019-04-09 Omniome, Inc. Methods for determining sequence profiles
WO2017123533A1 (en) 2016-01-11 2017-07-20 Illumina, Inc. Detection apparatus having a microfluorometer, a fluidic system, and a flow cell latch clamp module
PL3377226T3 (pl) 2016-03-28 2021-07-26 Illumina, Inc. Wielopłaszczyznowe mikromacierze
SG11201809376WA (en) 2016-05-18 2018-12-28 Illumina Inc Self assembled patterning using patterned hydrophobic surfaces
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
EP4439045A3 (de) 2016-10-14 2024-11-27 Illumina, Inc. Kartuschenanordnung
WO2018093780A1 (en) 2016-11-16 2018-05-24 Illumina, Inc. Validation methods and systems for sequence variant calls
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
WO2018148724A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus furiosus
WO2018148727A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from 9°n
WO2018148726A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from phage t4
EP3580351A1 (de) 2017-02-13 2019-12-18 Qiagen Sciences, LLC Polymeraseenzym aus 9°n
WO2018148723A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus abyssi
US11492666B2 (en) 2017-02-15 2022-11-08 Pacific Biosciences Of California, Inc. Distinguishing sequences by detecting polymerase dissociation
US10737267B2 (en) 2017-04-04 2020-08-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
SG11202000964UA (en) 2017-08-15 2020-02-27 Omniome Inc Scanning apparatus and methods useful for detection of chemical and biological analytes
NZ759818A (en) 2017-10-16 2022-04-29 Illumina Inc Semi-supervised learning for training an ensemble of deep convolutional neural networks
KR20220031940A (ko) 2017-10-16 2022-03-14 일루미나, 인코포레이티드 컨볼루션 신경망(cnn)을 사용하는 비정상 스플라이싱 검출
US11861491B2 (en) 2017-10-16 2024-01-02 Illumina, Inc. Deep learning-based pathogenicity classifier for promoter single nucleotide variants (pSNVs)
CA3079411C (en) 2017-10-19 2023-12-05 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
MX389484B (es) 2017-11-13 2025-03-20 The Multiple Myeloma Res Foundation Inc Base de datos, de datos moleculares, omicos, de inmunoterapia, metabolicos, epigeneticos y clinicos, integrados
CA3279137A1 (en) 2018-01-08 2025-10-30 Illumina, Inc. Systems and devices for high-throughput sequencing with semiconductor-based detection
WO2019136376A1 (en) 2018-01-08 2019-07-11 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
EP3619653B1 (de) 2018-01-15 2021-05-19 Illumina Inc. Auf tiefenlernen basierender variantenklassifikator
KR20230128411A (ko) 2018-03-22 2023-09-04 일루미나, 인코포레이티드 Rna 및 dna로부터의 핵산 라이브러리의 제조
NL2020861B1 (en) 2018-04-12 2019-10-22 Illumina Inc Variant classifier based on deep neural networks
US20190318806A1 (en) 2018-04-12 2019-10-17 Illumina, Inc. Variant Classifier Based on Deep Neural Networks
CA3097583A1 (en) 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
WO2019209426A1 (en) 2018-04-26 2019-10-31 Omniome, Inc. Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
US12084474B2 (en) 2018-05-15 2024-09-10 Illumina, Inc. Compositions and methods for chemical cleavage and deprotection of surface-bound oligonucleotides
AU2019276719B2 (en) 2018-05-31 2025-11-13 Pacific Biosciences Of California, Inc. Increased signal to noise in nucleic acid sequencing
US12073922B2 (en) 2018-07-11 2024-08-27 Illumina, Inc. Deep learning-based framework for identifying sequence patterns that cause sequence-specific errors (SSEs)
WO2020023362A1 (en) 2018-07-24 2020-01-30 Omniome, Inc. Serial formation of ternary complex species
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP4603594A3 (de) 2018-08-28 2025-11-12 10X Genomics, Inc. Erhöhung der räumlichen array-auflösung
JP6888123B2 (ja) 2018-10-15 2021-06-16 イルミナ インコーポレイテッド 深層畳み込みニューラルネットワークを事前訓練するための深層学習ベースの技術
WO2020101795A1 (en) 2018-11-15 2020-05-22 Omniome, Inc. Electronic detection of nucleic acid structure
CN211311438U (zh) 2018-12-04 2020-08-21 欧姆尼欧美公司 流动池
US20220290217A1 (en) 2018-12-10 2022-09-15 10X Genomics, Inc. Resolving spatial arrays using deconvolution
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
BR112020026667A2 (pt) 2018-12-19 2021-07-27 Illumina, Inc. métodos para melhorar a prioridade de clonalidade do agrupamento de polinucleotídeo
WO2020132350A2 (en) 2018-12-20 2020-06-25 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020167574A1 (en) 2019-02-14 2020-08-20 Omniome, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
CN113613787B (zh) 2019-02-20 2023-06-13 加利福尼亚太平洋生物科学股份有限公司 用于检测化学和生物分析物的扫描装置和方法
US11783917B2 (en) 2019-03-21 2023-10-10 Illumina, Inc. Artificial intelligence-based base calling
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
WO2020191389A1 (en) 2019-03-21 2020-09-24 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
WO2020227143A1 (en) * 2019-05-03 2020-11-12 Ultima Genomics, Inc. Fast-forward sequencing by synthesis methods
AU2020273459A1 (en) 2019-05-16 2021-01-07 Illumina, Inc. Base calling using convolutions
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
US11423306B2 (en) 2019-05-16 2022-08-23 Illumina, Inc. Systems and devices for characterization and performance analysis of pixel-based sequencing
EP3976820A1 (de) 2019-05-30 2022-04-06 10X Genomics, Inc. Verfahren zum nachweis der räumlichen heterogenität einer biologischen probe
WO2020252186A1 (en) 2019-06-11 2020-12-17 Omniome, Inc. Calibrated focus sensing
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
TW202124406A (zh) 2019-09-10 2021-07-01 美商歐姆尼歐美公司 核苷酸之可逆修飾
WO2021076152A1 (en) 2019-10-18 2021-04-22 Omniome, Inc. Methods and compositions for capping nucleic acids
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
EP4081656A1 (de) 2019-12-23 2022-11-02 10X Genomics, Inc. Zusammensetzungen und verfahren zur verwendung von fixierten biologischen proben bei partitionsbasierten tests
EP4081777B1 (de) 2019-12-23 2026-02-18 10X Genomics, Inc. Reversible fixierreagenzien und verfahren zu deren verwendung
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
WO2021158511A1 (en) 2020-02-04 2021-08-12 Omniome, Inc. Flow cells and methods for their manufacture and use
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US12591780B2 (en) 2020-02-20 2026-03-31 Illumina, Inc. Data compression for artificial intelligence-based base calling
KR20220143854A (ko) 2020-02-20 2022-10-25 일루미나, 인코포레이티드 인공 지능 기반 다-대-다 염기 호출
US12354008B2 (en) 2020-02-20 2025-07-08 Illumina, Inc. Knowledge distillation and gradient pruning-based compression of artificial intelligence-based base caller
US12592298B2 (en) 2020-02-20 2026-03-31 Illumina, Inc. Hardware execution and acceleration of artificial intelligence-based base caller
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
PL3969618T3 (pl) 2020-03-09 2024-01-03 Illumina, Inc. Sposoby sekwencjonowania polinukleotydów
CN114026250B (zh) 2020-03-09 2025-05-16 因美纳有限公司 核酸测序的改善
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
CN115836135A (zh) 2020-05-05 2023-03-21 加利福尼亚太平洋生物科学股份有限公司 用于修饰聚合酶-核酸复合物的组合物和方法
EP4414459B1 (de) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4446430A3 (de) 2020-06-10 2024-12-18 10X Genomics, Inc. Verfahren zur bestimmung einer position eines analyten in einer biologischen probe
WO2021259881A1 (en) 2020-06-22 2021-12-30 Illumina Cambridge Limited Nucleosides and nucleotides with 3' acetal blocking group
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4121555B1 (de) 2020-12-21 2026-02-25 10X Genomics, Inc. Verfahren zur erfassung von sonden und/oder barcodes
US12525320B2 (en) 2021-03-16 2026-01-13 Illumina, Inc. Neural network parameter quantization for base calling
WO2022197754A1 (en) 2021-03-16 2022-09-22 Illumina Software, Inc. Neural network parameter quantization for base calling
CA3210451A1 (en) 2021-03-22 2022-09-29 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
EP4428246B1 (de) 2021-04-14 2025-12-24 10X Genomics, Inc. Verfahren zur messung der fehllokalisierung eines analyten
US12217829B2 (en) 2021-04-15 2025-02-04 Illumina, Inc. Artificial intelligence-based analysis of protein three-dimensional (3D) structures
US12444482B2 (en) 2021-04-15 2025-10-14 Illumina, Inc. Multi-channel protein voxelization to predict variant pathogenicity using deep convolutional neural networks
EP4320271B1 (de) 2021-05-06 2025-03-19 10X Genomics, Inc. Verfahren zur erhöhung der auflösung von räumlicher analyse
CN117916390A (zh) 2021-05-20 2024-04-19 伊鲁米纳公司 用于边合成边测序的组合物和方法
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US12570831B2 (en) 2021-06-15 2026-03-10 Illumina, Inc. Hydrogel-free surface functionalization for sequencing
WO2023278184A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Methods and systems to correct crosstalk in illumination emitted from reaction sites
AU2022300970A1 (en) 2021-06-29 2024-01-18 Illumina, Inc. Self-learned base caller, trained using oligo sequences
US12530882B2 (en) 2021-07-01 2026-01-20 Illumina, Inc. Efficient artificial intelligence-based base calling of index sequences
US12423815B2 (en) 2021-07-13 2025-09-23 Illumina, Inc. Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
WO2023003757A1 (en) 2021-07-19 2023-01-26 Illumina Software, Inc. Intensity extraction with interpolation and adaptation for base calling
WO2023004357A1 (en) 2021-07-23 2023-01-26 Illumina, Inc. Methods for preparing substrate surface for dna sequencing
CA3223746A1 (en) 2021-07-28 2023-02-02 Rohan PAUL Quality score calibration of basecalling systems
KR20240035413A (ko) 2021-08-03 2024-03-15 일루미나, 인코포레이티드 다중 염기 호출자 모델을 사용한 염기 호출
CN118103750A (zh) 2021-08-31 2024-05-28 伊鲁米纳公司 具有增强的孔成像分辨率的流动池
EP4196605B1 (de) 2021-09-01 2024-12-04 10X Genomics, Inc. Verfahren zur blockierung einer erfassungssonde auf einer räumlichen anordnung
CA3234961A1 (en) 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
US20250163491A1 (en) 2021-12-16 2025-05-22 Illumina, Inc. Methods for metal directed cleavage of surface-bound polynucleotides
WO2023114397A1 (en) 2021-12-16 2023-06-22 Illumina, Inc. Hybrid clustering
CA3223615A1 (en) 2021-12-17 2023-06-22 Fei Shen Orthogonal hybridization
US20240401127A1 (en) 2021-12-20 2024-12-05 Illumina, Inc. Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
AU2022421156A1 (en) 2021-12-20 2024-01-18 Illumina, Inc. Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
AU2023208743A1 (en) 2022-01-20 2024-01-04 Illumina, Inc. Methods of detecting methylcytosine and hydroxymethylcytosine by sequencing
WO2023175037A2 (en) 2022-03-15 2023-09-21 Illumina, Inc. Concurrent sequencing of forward and reverse complement strands on separate polynucleotides for methylation detection
WO2023175026A1 (en) 2022-03-15 2023-09-21 Illumina, Inc. Methods of determining sequence information
US20230313294A1 (en) 2022-03-30 2023-10-05 Illumina Cambridge Limited Methods for chemical cleavage of surface-bound polynucleotides
US20250361558A1 (en) 2022-08-19 2025-11-27 Illumina, Inc. Third dna base pair site-specific dna detection
WO2024057280A1 (en) 2022-09-16 2024-03-21 Illumina Cambridge Limited Nanoparticle with polynucleotide binding site and method of making thereof
JP2025528992A (ja) 2022-09-19 2025-09-04 イルミナ インコーポレイテッド 固定化プライマーを含む変形可能なポリマー
US20240102067A1 (en) 2022-09-26 2024-03-28 Illumina, Inc. Resynthesis Kits and Methods
US20240124914A1 (en) 2022-09-30 2024-04-18 Illumina, Inc. Thermophilic compositions for nucleic acid amplification
US20240110234A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Amplification Compositions and Methods
US20240124929A1 (en) 2022-09-30 2024-04-18 Illumina, Inc. Mesophilic compositions for nucleic acid amplification
US20240110221A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of modulating clustering kinetics
WO2024102809A1 (en) 2022-11-09 2024-05-16 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
WO2024129969A1 (en) 2022-12-14 2024-06-20 Illumina, Inc. Systems and methods for capture and enrichment of clustered beads on flow cell substrates
AU2023409219A1 (en) 2022-12-22 2024-10-03 Illumina, Inc. Palladium catalyst compositions and methods for sequencing by synthesis
CA3246536A1 (en) 2022-12-22 2024-06-27 Illumina, Inc. TRANSITION METAL CATALYST COMPOSITIONS AND SYNTHETIC SEQUENCE PROCESSES
CN119095984A (zh) 2022-12-27 2024-12-06 伊路米纳有限公司 使用3'烯丙基封端的核苷酸的测序方法
EP4728091A1 (de) 2023-06-14 2026-04-22 Illumina, Inc. Gleichzeitige sequenzierung mit räumlich getrennten ringen
WO2024256581A1 (en) 2023-06-14 2024-12-19 Illumina, Inc. Determination of modified cytosines
WO2025062001A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Optimised nucleic acid sequencing
WO2025062002A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Concurrent sequencing using nick translation
WO2025072683A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Solid supports and methods for strand invasion-based amplification
WO2025137479A1 (en) 2023-12-22 2025-06-26 Illumina, Inc. Reusable flow cells and methods of using them for nucleic acid sequencing
WO2025240905A1 (en) 2024-05-17 2025-11-20 The Broad Institute, Inc. Imaging-free high-resolution spatial macromolecule abundance reconstruction
WO2026035914A1 (en) 2024-08-09 2026-02-12 Illumina, Inc. Nucleotides having 3'-hydroxy blocking groups
WO2026090042A1 (en) 2024-10-21 2026-04-30 The Broad Institute, Inc. Single-cell and spatial dna methylation measurements
WO2026097017A2 (en) 2024-11-01 2026-05-07 Illumina, Inc. Methods of improving sequencing accuracy

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0450060A1 (de) * 1989-10-26 1991-10-09 Sri International Dns-sequenzierung
DE4141178A1 (de) 1991-12-13 1993-06-17 Europ Lab Molekularbiolog Verfahren zur sequenzierung von nukleinsaeuren
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
JPH07203998A (ja) * 1994-01-26 1995-08-08 Hamamatsu Photonics Kk 核酸塩基配列決定方法
AU5171696A (en) * 1995-02-27 1996-09-18 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
JPH11503611A (ja) * 1995-04-11 1999-03-30 トラスティーズ・オブ・ボストン・ユニバーシティ 生体高分子の固相配列決定法
DE19515552A1 (de) * 1995-04-27 1996-10-31 Europ Lab Molekularbiolog Simultane Sequenzierung von Nukleinsäuren
AU5797696A (en) * 1995-05-19 1996-11-29 Ely Michael Rabani Parallel multiplex polynucleotide sequencing

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