DK0703991T3 - DNA-sekventering ved trinvis ligering og spaltning - Google Patents

DNA-sekventering ved trinvis ligering og spaltning

Info

Publication number
DK0703991T3
DK0703991T3 DK95916916T DK95916916T DK0703991T3 DK 0703991 T3 DK0703991 T3 DK 0703991T3 DK 95916916 T DK95916916 T DK 95916916T DK 95916916 T DK95916916 T DK 95916916T DK 0703991 T3 DK0703991 T3 DK 0703991T3
Authority
DK
Denmark
Prior art keywords
cleavage
dna fragments
target polynucleotide
ligation
nucleotides
Prior art date
Application number
DK95916916T
Other languages
Danish (da)
English (en)
Inventor
Sydney University Of C Brenner
Original Assignee
Lynx Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lynx Therapeutics Inc filed Critical Lynx Therapeutics Inc
Application granted granted Critical
Publication of DK0703991T3 publication Critical patent/DK0703991T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6862Ligase chain reaction [LCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/582Recycling of unreacted starting or intermediate materials

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK95916916T 1994-04-04 1995-03-24 DNA-sekventering ved trinvis ligering og spaltning DK0703991T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22230094A 1994-04-04 1994-04-04
US08/280,441 US5552278A (en) 1994-04-04 1994-07-25 DNA sequencing by stepwise ligation and cleavage
PCT/US1995/003678 WO1995027080A2 (fr) 1994-04-04 1995-03-24 Sequençage d'adn par ligature et clivage par etapes

Publications (1)

Publication Number Publication Date
DK0703991T3 true DK0703991T3 (da) 2001-06-18

Family

ID=26916666

Family Applications (1)

Application Number Title Priority Date Filing Date
DK95916916T DK0703991T3 (da) 1994-04-04 1995-03-24 DNA-sekventering ved trinvis ligering og spaltning

Country Status (12)

Country Link
US (3) US5552278A (fr)
EP (1) EP0703991B1 (fr)
JP (3) JP3872812B2 (fr)
AT (1) ATE201238T1 (fr)
AU (1) AU685628B2 (fr)
CA (1) CA2163662A1 (fr)
DE (1) DE69520917T2 (fr)
DK (1) DK0703991T3 (fr)
ES (1) ES2159635T3 (fr)
GR (1) GR3036314T3 (fr)
PT (1) PT703991E (fr)
WO (1) WO1995027080A2 (fr)

Families Citing this family (336)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993017719A1 (fr) * 1992-03-13 1993-09-16 Diatech, Inc. PEPTIDES MARQUES AU TECHNETIUM-99m POUR LA MISE EN IMAGE D'UNE INFLAMMATION
GB9401200D0 (en) * 1994-01-21 1994-03-16 Medical Res Council Sequencing of nucleic acids
US5714330A (en) * 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US6013445A (en) * 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5695934A (en) * 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US8236493B2 (en) * 1994-10-21 2012-08-07 Affymetrix, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
US6974666B1 (en) * 1994-10-21 2005-12-13 Appymetric, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
US5866330A (en) * 1995-09-12 1999-02-02 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
US5695937A (en) * 1995-09-12 1997-12-09 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
US5763175A (en) * 1995-11-17 1998-06-09 Lynx Therapeutics, Inc. Simultaneous sequencing of tagged polynucleotides
US5780231A (en) * 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
US6537776B1 (en) * 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US5851772A (en) * 1996-01-29 1998-12-22 University Of Chicago Microchip method for the enrichment of specific DNA sequences
PL331513A1 (en) * 1996-06-06 1999-07-19 Lynx Therapeutics Method of sequencing, by a ligand effect, specific encoded adapters and composition containing double-string oligonucleotidic adapters
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9620769D0 (en) * 1996-10-04 1996-11-20 Brax Genomics Ltd Nucleic acid sequencing
US6124092A (en) * 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
US6258533B1 (en) * 1996-11-01 2001-07-10 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US5858671A (en) * 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
WO1998035012A2 (fr) * 1997-02-12 1998-08-13 Chan Eugene Y Procedes et produits permettant d'analyser des polymeres
IL120339A0 (en) * 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Improved DNA assembly method
US6197557B1 (en) 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US6117634A (en) 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
US5994068A (en) 1997-03-11 1999-11-30 Wisconsin Alumni Research Foundation Nucleic acid indexing
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
US5888737A (en) * 1997-04-15 1999-03-30 Lynx Therapeutics, Inc. Adaptor-based sequence analysis
JP4294740B2 (ja) * 1997-05-23 2009-07-15 ソレクサ・インコーポレイテッド 分析物の系列的プロセシングのためのシステムおよび装置
ATE289358T1 (de) * 1997-06-27 2005-03-15 Lynx Therapeutics Inc Verfahren zur restriktionskarten-analyse von polynukleotiden
JP4789294B2 (ja) * 1997-08-22 2011-10-12 ソレクサ・インコーポレイテッド ローリングプライマーを用いたdna伸長および分析
US6607878B2 (en) 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
GB9803930D0 (en) * 1998-02-26 1998-04-22 Univ Glasgow Polynucleotide sequencing method and kits therefor
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
DE19822287C2 (de) * 1998-05-18 2003-04-24 Switch Biotech Ag Klonierungsvektor, seine Herstellung und Verwendung zur Analyse von mRNA Expressionsmuster
EP1090293B2 (fr) 1998-06-24 2019-01-23 Illumina, Inc. Décodage de détecteurs matricels à microspheres
US6162602A (en) * 1998-07-16 2000-12-19 Gautsch; James W. Automatic direct sequencing of bases in nucleic acid chain elongation
US7270958B2 (en) * 1998-09-10 2007-09-18 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US6440705B1 (en) 1998-10-01 2002-08-27 Vincent P. Stanton, Jr. Method for analyzing polynucleotides
US6855500B2 (en) 1998-10-01 2005-02-15 Sequenom, Inc. Fluorescence-based genotyping
US6777188B2 (en) 1998-10-01 2004-08-17 Variagenics, Inc. Genotyping by mass spectrometric analysis of allelic fragments
US6458945B1 (en) 1998-10-01 2002-10-01 Variagenics, Inc. Method for analyzing polynucleotides
US6994998B1 (en) 1998-10-01 2006-02-07 Sequenom, Inc. Base-modified nucleotides and their use for polymorphism detection
US6500650B1 (en) * 1998-10-01 2002-12-31 Variagenics, Inc. Method for identifying polymorphisms
US6610492B1 (en) 1998-10-01 2003-08-26 Variagenics, Inc. Base-modified nucleotides and cleavage of polynucleotides incorporating them
US6566059B1 (en) 1998-10-01 2003-05-20 Variagenics, Inc. Method for analyzing polynucleotides
WO2000036152A1 (fr) 1998-12-14 2000-06-22 Li-Cor, Inc. Systeme et procede de sequençage d'acides nucleiques mono-moleculaires par synthese de polymerase
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
EP1147220A1 (fr) * 1999-01-29 2001-10-24 Keygene N.V. Methode et dispositif permettant de determiner une ou plusieurs enzymes de restriction en vue de l'analyse d'un acide nucleique ou d'une sequence nucleotidique
JP2002537856A (ja) * 1999-03-05 2002-11-12 トランジェーヌ ヌクレオチド断片、プローブ、プライマー、試薬、及びpBR322の複製起点由来のヌクレオチド配列を検出する方法
US6521428B1 (en) * 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
EP1190100B1 (fr) * 1999-05-20 2012-07-25 Illumina, Inc. Decodage combinatoire de jeux d'acides nucleiques aleatoires
US20020119448A1 (en) * 1999-06-23 2002-08-29 Joseph A. Sorge Methods of enriching for and identifying polymorphisms
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
WO2001012855A2 (fr) * 1999-08-13 2001-02-22 Yale University Etiquette de sequence a codage binaire
GB0016473D0 (en) * 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method
GB0016472D0 (en) * 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method and apparatus
US20060057565A1 (en) * 2000-09-11 2006-03-16 Jingyue Ju Combinatorial fluorescence energy transfer tags and uses thereof
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
AU2001296645A1 (en) 2000-10-06 2002-04-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
AU2002227156A1 (en) 2000-12-01 2002-06-11 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
US6949340B2 (en) 2001-03-28 2005-09-27 Creative Mines Llc Optical phase modulator
EP1262563A3 (fr) * 2001-05-31 2003-02-05 Takara Bio Inc. Méthode de fixation d'ADN sur une surface
US6613523B2 (en) * 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
WO2003056030A2 (fr) * 2001-11-08 2003-07-10 The Johns Hopkins University Procedes et systemes pour le sequençage d'acides nucleiques
US20030166026A1 (en) * 2002-01-09 2003-09-04 Lynx Therapeutics, Inc. Identification of specific biomarkers for breast cancer cells
US20030180764A1 (en) * 2002-01-09 2003-09-25 Lynx Therapeutics, Inc. Genes affected by cholesterol treatment and during adipogenesis
US20030170700A1 (en) * 2002-01-09 2003-09-11 Lynx Therapeutics, Inc. Secreted and cell surface polypeptides affected by cholesterol and uses thereof
US6723546B2 (en) * 2002-03-26 2004-04-20 New England Biolabs, Inc. Method for cloning and expression of BsaI restriction endonuclease and BsaI methylase in E. coli
US7291460B2 (en) * 2002-05-31 2007-11-06 Verenium Corporation Multiplexed systems for nucleic acid sequencing
US7074597B2 (en) * 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
JP2006517090A (ja) * 2002-09-26 2006-07-20 コーサン バイオサイエンシーズ, インコーポレイテッド 合成遺伝子
WO2004055160A2 (fr) * 2002-12-13 2004-07-01 The Trustees Of Columbia University In The City Of New York Methodes de couplage biomoleculaire par cycloaddition 1,3-dipolaire
US20040224330A1 (en) * 2003-01-15 2004-11-11 Liyan He Nucleic acid indexing
WO2005010184A1 (fr) * 2003-07-25 2005-02-03 Takara Bio Inc. Methode servant a detecter une mutation
WO2005017186A2 (fr) * 2003-08-08 2005-02-24 Thomas Jefferson University Procede d'identification rapide d'epissage alterne
US20050094807A1 (en) * 2003-11-04 2005-05-05 John Silzel Accuracy array assay system and method
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005080604A2 (fr) * 2004-02-12 2005-09-01 Compass Genetics, Llc Analyse genetique par tri specifique de sequences
EP2436778A3 (fr) * 2004-03-03 2012-07-11 The Trustees of Columbia University in the City of New York Nucleotides fluorescents photoclivables destinés à séquencer de l'ADN sur une puce construite par chimie de couplage specifique au site
US20050227316A1 (en) * 2004-04-07 2005-10-13 Kosan Biosciences, Inc. Synthetic genes
WO2006073436A2 (fr) * 2004-04-29 2006-07-13 The Trustees Of Columbia University In The City Of New York Pcr a marqueur de masse permettant de proceder a un diagnostic multiplex
GB0422551D0 (en) * 2004-10-11 2004-11-10 Univ Liverpool Labelling and sequencing of nucleic acids
EP2241637A1 (fr) * 2005-02-01 2010-10-20 AB Advanced Genetic Analysis Corporation Sequençage nucléotidique comprenant des cycles successifs de formation duplex
EP2230316A1 (fr) 2005-02-01 2010-09-22 AB Advanced Genetic Analysis Corporation Sequencage nucléotidique comprenant des cycles sucessifs de fromation duplex
US7407757B2 (en) * 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
WO2006099604A2 (fr) * 2005-03-16 2006-09-21 Compass Genetics, Llc Procedes et compositions pour releves de mesures sur de multiples plate-formes analytiques
US9169510B2 (en) * 2005-06-21 2015-10-27 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compositions
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20070065834A1 (en) * 2005-09-19 2007-03-22 Hillis William D Method and sequences for determinate nucleic acid hybridization
WO2007062105A2 (fr) * 2005-11-21 2007-05-31 The Trustees Of Columbia University In The City Of New York Immunocapture numerique multiplex utilisant une bibliotheque de marqueurs de masse photoclivable
JPWO2007097443A1 (ja) * 2006-02-20 2009-07-16 国立大学法人 北海道大学 Dna塩基配列を決定する方法
CN101495654A (zh) * 2006-04-19 2009-07-29 阿普里拉股份有限公司 无凝胶珠基测序的试剂、方法和文库
US20090002703A1 (en) * 2006-08-16 2009-01-01 Craig Edward Parman Methods and systems for quantifying isobaric labels and peptides
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US7947446B2 (en) * 2007-05-29 2011-05-24 Ming-Sheng Lee High throughput mutation screening methods and kits using a universalized approach—differential sequence fill-in (DSF)-enabled sequential adapter ligation and amplification
JP2010537643A (ja) 2007-08-29 2010-12-09 アプライド バイオシステムズ, エルエルシー 代替的な核酸配列決定法
WO2009051807A1 (fr) 2007-10-19 2009-04-23 The Trustees Of Columbia University In The City Of New York Conception et synthèse de nucléotides fluorescents clivables en tant que terminateurs réversibles pour le séquençage de l'adn par synthèse
DK2725107T3 (da) 2007-10-19 2019-01-02 Univ Columbia DNA-sekventering med ikke-fluorescerende nukleotidreversible terminatorer og ddNTP'er modificeret med spaltbart mærke og nukleinsyre omfattende inosin med reversible terminatorer
US8101353B2 (en) * 2007-12-18 2012-01-24 Advanced Analytical Technologies, Inc. System and method for nucleotide sequence profiling for sample identification
WO2009086353A1 (fr) * 2007-12-26 2009-07-09 Helicos Biosciences Corporation Séquençage à deux amorces amélioré pour analyse d'expression de haute capacité
US8039817B2 (en) 2008-05-05 2011-10-18 Illumina, Inc. Compensator for multiple surface imaging
EP2294214A2 (fr) * 2008-05-07 2011-03-16 Illumina, Inc. Compositions et procédés pour fournir des substances à une matrice et provenant de celle-ci
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
WO2010068702A2 (fr) * 2008-12-10 2010-06-17 Illumina, Inc. Procédés et compositions pour hybrider des acides nucléiques
GB0904934D0 (en) 2009-03-23 2009-05-06 Geneseqe As Method and apparatus for detecting molecules
US20110070590A1 (en) 2009-09-22 2011-03-24 Jan Rohozinski Primers and Methods for Determining RhD Zygosity
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
WO2011127099A1 (fr) 2010-04-05 2011-10-13 Prognosys Biosciences, Inc. Tests biologiques à codage spatial
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US10240194B2 (en) 2010-05-13 2019-03-26 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
US8483969B2 (en) 2010-09-17 2013-07-09 Illuminia, Inc. Variation analysis for multiple templates on a solid support
WO2012050920A1 (fr) 2010-09-29 2012-04-19 Illumina, Inc. Compositions et procédés de séquençage d'acides nucléiques
GB201016484D0 (en) 2010-09-30 2010-11-17 Geneseque As Method
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
CN103443338B (zh) 2011-02-02 2017-09-22 华盛顿大学商业化中心 大规模平行邻接作图
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP2718465B1 (fr) 2011-06-09 2022-04-13 Illumina, Inc. Procede de fabrication d'un reseau d'analytes
EP3305400A3 (fr) 2011-10-28 2018-06-06 Illumina, Inc. Système et procédé de fabrication de microréseau
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
GB201215435D0 (en) * 2012-08-30 2012-10-17 Geneseque As Sequencing method
EP4592400A3 (fr) 2012-10-17 2025-10-29 10x Genomics Sweden AB Procédés et produits pour optimiser la detection localisée ou spatiale de l'expression génique dans un échantillon de tissu
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
DK2969479T3 (da) 2013-03-13 2021-08-02 Illumina Inc Væskeanordninger i flere lag og fremgangsmåder til deres fremstilling
DK2970951T3 (da) 2013-03-13 2019-05-13 Illumina Inc Fremgangsmåder til nukleinsyresekventering
WO2014144883A1 (fr) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Molecules marquees par des amas de raman destinees a l'imagerie biologique
WO2014139596A1 (fr) 2013-03-15 2014-09-18 Illumina Cambridge Limited Nucléosides ou nucléotides modifiés
US9193998B2 (en) 2013-03-15 2015-11-24 Illumina, Inc. Super resolution imaging
CA2916662C (fr) 2013-06-25 2022-03-08 Prognosys Biosciences, Inc. Procedes et systemes pour determiner des motifs spatiales de cibles biologiques dans un echantillon
DK3017065T3 (en) 2013-07-01 2018-11-26 Illumina Inc Catalyst-free Surface functionalization and polymer grafting
CN105358715B (zh) * 2013-07-03 2018-09-18 伊鲁米那股份有限公司 正交合成测序
KR20160081896A (ko) 2013-08-30 2016-07-08 일루미나, 인코포레이티드 친수성 또는 다양한-친수성 표면 상에서의 액적의 조작
US9352315B2 (en) 2013-09-27 2016-05-31 Taiwan Semiconductor Manufacturing Company, Ltd. Method to produce chemical pattern in micro-fluidic structure
EP3957750B1 (fr) 2013-12-20 2025-01-29 Illumina, Inc. Conservation des informations de connectivité génomique dans des échantillons d'adn génomiques fragmentés
JP7082860B2 (ja) 2013-12-23 2022-06-09 イラミーナ インコーポレーテッド 光放射の検出を改善するための構造化基板および同構造化基板に関する方法
US10934581B2 (en) 2014-04-30 2021-03-02 International Business Machines Corporation Bow tie DNA compositions and methods
CN106715693A (zh) 2014-06-13 2017-05-24 伊卢米纳剑桥有限公司 用于制备序列文库的方法和组合物
CN112430641A (zh) 2014-06-30 2021-03-02 亿明达股份有限公司 使用单侧转座的方法和组合物
CN113913505A (zh) 2014-07-15 2022-01-11 亿明达股份有限公司 生物化学激活的电子装置
GB201413929D0 (en) 2014-08-06 2014-09-17 Geneseque As Method
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
US9982250B2 (en) 2014-08-21 2018-05-29 Illumina Cambridge Limited Reversible surface functionalization
RU2736728C2 (ru) 2014-10-17 2020-11-19 Иллумина Кембридж Лимитед Транспозиция с сохранением сцепления генов
WO2016066586A1 (fr) 2014-10-31 2016-05-06 Illumina Cambridge Limited Nouveaux polymères et revêtements de copolymères d'adn
EP3901282B1 (fr) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Analyse de plusieurs acides nucléiques spatialement différenciés de spécimens biologiques
EP3696536A1 (fr) 2015-04-14 2020-08-19 Illumina, Inc. Procédé de fabrication d'un substrat et procédé d'analyse de biomolécules aptes à générer des émissions de lumière
RU2724998C2 (ru) 2015-05-11 2020-06-29 Иллюмина, Инк. Платформа для обнаружения и анализа терапевтических агентов
US10808282B2 (en) 2015-07-07 2020-10-20 Illumina, Inc. Selective surface patterning via nanoimprinting
EP3325648B1 (fr) 2015-07-17 2023-03-29 Illumina, Inc. Feuilles polymères pour applications de séquençage
WO2017019278A1 (fr) 2015-07-30 2017-02-02 Illumina, Inc. Déblocage orthogonal de nucléotides
HK1253910A1 (zh) 2015-09-02 2019-07-05 伊卢米纳剑桥有限公司 改善流控系统中的液滴操作的系统和方法
CN109072300B (zh) 2015-12-17 2023-01-31 伊路敏纳公司 区分复杂生物样品中的甲基化水平
US10907195B1 (en) * 2016-02-16 2021-02-02 Pharmaseq, Inc. Devices and methods for assembling extremely long DNA molecules
PL3377226T3 (pl) 2016-03-28 2021-07-26 Illumina, Inc. Wielopłaszczyznowe mikromacierze
WO2017177017A1 (fr) 2016-04-07 2017-10-12 Omniome, Inc. Procédés de quantification d'acides nucléiques cibles et d'identification de variants de séquences
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CN110249059B (zh) 2017-01-20 2021-04-13 欧姆尼欧美公司 核酸的等位基因特异性捕获
CA3050852C (fr) 2017-01-20 2021-03-09 Omniome, Inc. Genotypage par liaison par polymerase
WO2018187013A1 (fr) 2017-04-04 2018-10-11 Omniome, Inc. Appareil fluidique et procédés utiles pour des réactions chimiques et biologiques
WO2019035897A1 (fr) 2017-08-15 2019-02-21 Omniome, Inc. Appareil de balayage et procédés utiles pour la détection d'analytes chimiques ou biologiques
AU2018336785B2 (en) 2017-09-20 2022-07-14 Regeneron Pharmaceuticals, Inc. Immunotherapy methods for patients whose tumors carry a high passenger gene mutation burden
WO2019203986A1 (fr) 2018-04-19 2019-10-24 Omniome, Inc. Amélioration de la précision d'appels de base dans des procédés de séquençage d'acide nucléique
NL2021377B1 (en) 2018-07-03 2020-01-08 Illumina Inc Interposer with first and second adhesive layers
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US20210332424A1 (en) 2018-08-28 2021-10-28 10X Genomics, Inc. Methods of generating an array
WO2020047005A1 (fr) 2018-08-28 2020-03-05 10X Genomics, Inc. Résolution de réseaux spatiaux
EP3870704A4 (fr) 2018-10-25 2023-01-11 Illumina, Inc. Procédés et compositions pour identifier des ligands sur des réseaux à l'aide d'indices et de codes à barres
EP3891304A1 (fr) 2018-12-04 2021-10-13 Omniome, Inc. Fluides à phase mixte pour séquençage d'acide nucléique et autres essais analytiques
SG11202012762WA (en) 2018-12-05 2021-01-28 Illumina Cambridge Ltd Methods and compositions for cluster generation by bridge amplification
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
WO2020123316A2 (fr) 2018-12-10 2020-06-18 10X Genomics, Inc. Procédés de détermination d'un emplacement d'un analyte biologique dans un échantillon biologique
JP7587425B2 (ja) 2018-12-18 2024-11-20 イルミナ ケンブリッジ リミテッド 単一表面プライマーを使用したペアエンドシーケンシングのための方法および組成物
DK3899037T3 (da) 2018-12-19 2023-11-06 Illumina Inc Fremgangsmåder til forbedring af polynukleotidklyngeklonalitetsprioritet
EP3899032A2 (fr) 2018-12-20 2021-10-27 Omniome, Inc. Régulation de température pour l'analyse d'acides nucléiques et d'autres analytes
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020167574A1 (fr) 2019-02-14 2020-08-20 Omniome, Inc. Atténuation d'impacts défavorables de systèmes de détection sur des acides nucléiques et d'autres analytes biologiques
CN114174531A (zh) 2019-02-28 2022-03-11 10X基因组学有限公司 用空间条码化寡核苷酸阵列对生物分析物进行概况分析
EP3938538A1 (fr) 2019-03-15 2022-01-19 10X Genomics, Inc. Méthodes d'utilisation de matrices spatiales pour le séquençage d'organismes unicellulaires
EP3887542A1 (fr) 2019-03-22 2021-10-06 10X Genomics, Inc. Analyse spatiale tridimensionnelle
WO2020243579A1 (fr) 2019-05-30 2020-12-03 10X Genomics, Inc. Procédés de détection de l'hétérogénéité spatiale d'un échantillon biologique
US11644406B2 (en) 2019-06-11 2023-05-09 Pacific Biosciences Of California, Inc. Calibrated focus sensing
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
CN114728996B (zh) 2019-09-10 2022-11-29 加利福尼亚太平洋生物科学股份有限公司 核苷酸的可逆修饰
SG11202113372WA (en) 2019-09-20 2021-12-30 Illumina Inc Methods and compositions for identifying ligands on arrays using indexes and barcodes
EP4468252A3 (fr) 2019-10-01 2025-02-19 10x Genomics, Inc. Les systèmes et méthodes d'identification des caractéristiques morphologiques dans les échantillons de tissus
EP4045683B1 (fr) 2019-10-18 2025-02-19 Pacific Biosciences of California, Inc. Procédés pour le coiffage d'acides nucléiques
WO2021091611A1 (fr) 2019-11-08 2021-05-14 10X Genomics, Inc. Agents de capture d'analytes marqués spatialement pour le multiplexage d'analytes
WO2021092433A2 (fr) 2019-11-08 2021-05-14 10X Genomics, Inc. Amélioration de la spécificité de la liaison d'un analyte
WO2021102003A1 (fr) 2019-11-18 2021-05-27 10X Genomics, Inc. Systèmes et procédés de classification de tissu
AU2020388573A1 (en) 2019-11-21 2022-06-23 10X Genomics, Inc, Spatial analysis of analytes
DE202019106695U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
EP4081656A1 (fr) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions et méthodes d'utilisation d'échantillons biologiques fixés dans des dosages basés sur des compartiments
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
EP4081653B1 (fr) 2019-12-23 2024-07-03 Singular Genomics Systems, Inc. Méthodes de séquençage à longs fragments
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
EP4100161B1 (fr) 2020-02-04 2024-07-10 Pacific Biosciences of California, Inc. Cuves à circulation et procédés pour leur utilisation
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (fr) 2020-02-07 2021-08-12 10X Genomics, Inc. Évaluation quantitative et automatisée de la performance de perméabilisation pour la transcriptomique spatiale
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
CN115428088A (zh) 2020-02-13 2022-12-02 10X基因组学有限公司 用于基因表达和dna染色质可及性的联合交互式可视化的系统和方法
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
CN115485391A (zh) 2020-02-17 2022-12-16 10X基因组学有限公司 染色质相互作用的原位分析
EP4484571A3 (fr) 2020-02-21 2025-03-19 10X Genomics, Inc. Procédés et compositions pour un dosage spatial in situ intégré
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
CA3173798A1 (fr) 2020-03-03 2021-09-10 Pacific Biosciences Of California, Inc. Procedes et compositions de sequencage d'acides nucleiques bicatenaires
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
WO2021178893A2 (fr) 2020-03-06 2021-09-10 Singular Genomics Systems, Inc. Séquençage de brin apparié lié
EP4139485B1 (fr) 2020-04-22 2023-09-06 10X Genomics, Inc. Procédés d'analyse spatiale utilisant un appauvrissement d'arn ciblée
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
EP4414459B1 (fr) 2020-05-22 2025-09-03 10X Genomics, Inc. Mesure spatio-temporelle simultanée de l'expression génique et de l'activité cellulaire
EP4153776B1 (fr) 2020-05-22 2025-03-05 10X Genomics, Inc. Analyse spatiale pour détecter des variants de séquence
WO2021242834A1 (fr) 2020-05-26 2021-12-02 10X Genomics, Inc. Procédé de réinitialisation d'un réseau
EP4600376A3 (fr) 2020-06-02 2025-10-22 10X Genomics, Inc. Transcriptome spatial pour récepteurs d'antigènes
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (fr) 2020-06-08 2025-08-13 10X Genomics, Inc. Procédés de détermination d'une marge chirurgicale et procédés d'utilisation de ceux-ci
WO2021252375A1 (fr) 2020-06-08 2021-12-16 The Broad Institute, Inc. Indexation combinatoire à cellule unique, à partir d'acides nucléiques amplifiés
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
WO2021252747A1 (fr) 2020-06-10 2021-12-16 1Ox Genomics, Inc. Procédés de distribution de fluide
EP4165207B1 (fr) 2020-06-10 2024-09-25 10X Genomics, Inc. Methode pour determiner la position d'un analyte dans un echantillon biologique
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
ES2994976T3 (en) 2020-06-25 2025-02-05 10X Genomics Inc Spatial analysis of dna methylation
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
WO2022015600A2 (fr) 2020-07-13 2022-01-20 Singular Genomics Systems, Inc. Procédés de séquençage de polynucléotides complémentaires
US11492662B2 (en) 2020-08-06 2022-11-08 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US12580044B1 (en) 2020-09-02 2026-03-17 10X Genomics, Inc. Systems and methods for identifying cells that are antigen-specific for an immunogenic feature
WO2022061150A2 (fr) 2020-09-18 2022-03-24 10X Geonomics, Inc. Appareil de manipulation d'échantillons et procédés d'enregistrement d'images
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
WO2022087485A1 (fr) 2020-10-22 2022-04-28 Singular Genomics Systems, Inc. Circularisation et amplification d'acide nucléique sur une surface
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US12509717B2 (en) 2020-12-11 2025-12-30 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
EP4729631A2 (fr) 2020-12-21 2026-04-22 10X Genomics, Inc. Procédés, compositions et systèmes de capture de sondes et/ou de codes à barres
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
US20220235403A1 (en) 2021-01-26 2022-07-28 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
EP4251770A4 (fr) 2021-02-08 2024-05-29 Singular Genomics Systems, Inc. Procédés et compositions pour le séquençage de polynucléotides complémentaires
US20240043915A1 (en) 2021-02-13 2024-02-08 The General Hospital Corporation Methods and compositions for in situ macromolecule detection and uses thereof
EP4421491A3 (fr) 2021-02-19 2024-11-27 10X Genomics, Inc. Procédé d'utilisation d'un dispositif de support d'analyse modulaire
EP4301873A1 (fr) 2021-03-03 2024-01-10 10X Genomics, Inc. Détection d'analytes in situ à l'aide d'origami d'acide nucléique
EP4263868A4 (fr) 2021-03-12 2024-11-27 Singular Genomics Systems, Inc. Nanoréseaux et leurs procédés d'utilisation
US11884977B2 (en) 2021-03-12 2024-01-30 Singular Genomics Systems, Inc. Nanoarrays and methods of use thereof
AU2022238446A1 (en) 2021-03-18 2023-09-07 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
WO2022204032A1 (fr) 2021-03-22 2022-09-29 Illumina Cambridge Limited Procédés d'amélioration de la clonalité de groupes d'acides nucléiques
EP4305196B1 (fr) 2021-04-14 2025-04-02 10X Genomics, Inc. Procédés de mesure d'une mauvaise localisation d'un analyte
WO2022232308A1 (fr) 2021-04-27 2022-11-03 Singular Genomics Systems, Inc. Séquençage haute densité et amorçage multiplexé
EP4334706A4 (fr) 2021-05-05 2025-08-06 Singular Genomics Systems Inc Dispositif d'analyse multiomique et ses procédés d'utilisation
EP4320271B1 (fr) 2021-05-06 2025-03-19 10X Genomics, Inc. Procédés pour augmenter la résolution d'une analyse spatiale
WO2022256324A1 (fr) 2021-06-01 2022-12-08 10X Genomics, Inc. Procédés et compositions pour la détection d'analytes et la résolution de sondes
WO2022256422A1 (fr) 2021-06-02 2022-12-08 10X Genomics, Inc. Analyse d'échantillon à l'aide de sondes circularisables asymétriques
WO2022256503A1 (fr) 2021-06-03 2022-12-08 10X Genomics, Inc. Procédés, compositions, kits et systèmes pour améliorer la capture d'analytes pour une analyse spatiale
WO2023288225A1 (fr) 2021-07-13 2023-01-19 10X Genomics, Inc. Procédés de préparation d'une matrice polymérisée à épaisseur contrôlable
AU2022317053B2 (en) 2021-07-30 2025-01-02 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
US12553079B2 (en) 2021-08-03 2026-02-17 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
EP4446426B1 (fr) 2021-08-16 2026-03-04 10X Genomics, Inc. Sondes comprenant une région de code-barres divisée et procédés d'utilisation
WO2023023638A1 (fr) 2021-08-20 2023-02-23 Singular Genomics Systems, Inc. Procédés d'amplification d'acides nucléiques assistés par voie chimique et thermique
CN118103750A (zh) 2021-08-31 2024-05-28 伊鲁米纳公司 具有增强的孔成像分辨率的流动池
WO2023034489A1 (fr) 2021-09-01 2023-03-09 10X Genomics, Inc. Procédés, compositions et kits pour bloquer une sonde de capture sur un réseau spatial
WO2023076832A1 (fr) 2021-10-25 2023-05-04 Singular Genomics Systems, Inc. Manipulation et détection d'échantillons biologiques
EP4422792A4 (fr) 2021-10-26 2025-12-17 Singular Genomics Systems Inc Amplification ciblée multiplexée de polynucléotides
WO2023086880A1 (fr) 2021-11-10 2023-05-19 10X Genomics, Inc. Procédés, compositions et kits pour déterminer l'emplacement d'un analyte dans un échantillon biologique
WO2023102118A2 (fr) 2021-12-01 2023-06-08 10X Genomics, Inc. Procédés, compositions et systèmes pour la détection améliorée d'analytes in situ et analyse spatiale
WO2023122033A1 (fr) 2021-12-20 2023-06-29 10X Genomics, Inc. Auto-test pour dispositif d'imagerie
EP4423296A2 (fr) 2021-12-27 2024-09-04 10X Genomics, Inc. Procédés et compositions pour l'amplification par cercle roulant
WO2023126457A1 (fr) 2021-12-29 2023-07-06 Illumina Cambridge Ltd. Procédés de séquençage d'acide nucléique à l'aide d'amorces liées à une surface
EP4733410A2 (fr) 2022-01-21 2026-04-29 10X Genomics, Inc. Signaux de lecture multiples pour analyser un échantillon
US20250188521A1 (en) 2022-03-10 2025-06-12 Singular Genomics Systems, Inc. Nucleic acid delivery scaffolds
EP4505177A1 (fr) 2022-04-01 2025-02-12 10x Genomics, Inc. Compositions et procédés de masquage ciblé d'autofluorescence
US20230323427A1 (en) 2022-04-06 2023-10-12 10X Genomics, Inc. Methods and compositions for multiplex cell analysis
EP4519674A1 (fr) 2022-05-06 2025-03-12 10X Genomics, Inc. Analyse d'un antigène et des interactions antigène-récepteur
EP4540607A1 (fr) 2022-06-17 2025-04-23 10X Genomics, Inc. Dé-réticulation catalytique d'échantillons pour analyse in situ
WO2024015962A1 (fr) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Adaptateurs en épingle à cheveux asymétriques bloqués
US20240101978A1 (en) 2022-08-12 2024-03-28 10X Genomics, Inc. Puma1 polymerases and uses thereof
WO2024040060A1 (fr) 2022-08-16 2024-02-22 10X Genomics, Inc. Ap50 polymérases et leurs utilisations
WO2024081869A1 (fr) 2022-10-14 2024-04-18 10X Genomics, Inc. Procédés d'analyse d'échantillons biologiques
WO2024102736A1 (fr) 2022-11-08 2024-05-16 10X Genomics, Inc. Procédés et compositions d'immobilisation pour détection in situ
EP4482979B1 (fr) 2022-11-09 2025-10-01 10X Genomics, Inc. Procédés, compositions et kits pour déterminer l'emplacement de plusieurs analytes dans un échantillon biologique
EP4573210B1 (fr) 2022-11-16 2026-05-06 10X Genomics, Inc. Procédé pour analyser l'expression des gènes dans un pellet des céllules
EP4619736A1 (fr) 2022-11-18 2025-09-24 10X Genomics, Inc. Systèmes et procédés d'atténuation active de vibrations
EP4622746A1 (fr) 2022-11-22 2025-10-01 10X Genomics, Inc. Systèmes et procédés d'interfaçage avec une bouteille
US12581050B2 (en) 2022-11-23 2026-03-17 10X Genomics, Inc. Optical assemblies for machine vision calibration
WO2024129672A1 (fr) 2022-12-12 2024-06-20 The Broad Institute, Inc. Arn en circulation pour évaluer la connectivité cellule-cellule et la neuroanatomie
EP4634400A1 (fr) 2022-12-14 2025-10-22 Illumina, Inc. Systèmes et procédés de capture et d'enrichissement de billes en grappe sur des substrats de cuve à circulation
EP4634404A1 (fr) 2022-12-16 2025-10-22 10X Genomics, Inc. Procédés et compositions d'évaluation de performances
EP4638789A1 (fr) 2022-12-21 2025-10-29 10X Genomics, Inc. Procédés d'accroche d'acides ribonucléiques dans des échantillons biologiques
EP4705515A1 (fr) 2023-05-03 2026-03-11 10X Genomics, Inc. Procédés et compositions pour dosage spatial
EP4461816A1 (fr) 2023-05-10 2024-11-13 10X Genomics, Inc. Compositions d'acide boronique et procédés de fixation d'acides ribonucléiques dans des échantillons biologiques
WO2024249591A1 (fr) 2023-05-31 2024-12-05 Illumina, Inc. Procédés de séquençage double brin par synthèse
WO2025029831A1 (fr) 2023-07-31 2025-02-06 10X Genomics, Inc. Procédés et systèmes de clivage d'arn ciblé et d'amplification de cercle roulant amorcé par arn cible
WO2025111463A1 (fr) 2023-11-22 2025-05-30 10X Genomics, Inc. Procédés de traitement des acides ribonucléiques dans des échantillons biologiques
US20250207203A1 (en) 2023-12-20 2025-06-26 10X Genomics, Inc. In situ detection of copy number variations in biological samples
US20250288988A1 (en) 2024-03-15 2025-09-18 10X Genomics, Inc. Systems and methods for covering and sealing an open well
WO2025207535A1 (fr) 2024-03-26 2025-10-02 Illumina, Inc. Surfaces photocommutables
WO2025240918A1 (fr) 2024-05-17 2025-11-20 10X Genomics, Inc. Systèmes et procédés de génération de livres de codes
WO2025264702A1 (fr) 2024-06-18 2025-12-26 10X Genomics, Inc. Hydrogels 3d pour le séquençage d'acides nucléiques
WO2025264831A1 (fr) 2024-06-18 2025-12-26 Illumina, Inc. Procédés pour augmenter la qualité de séquençage de régions riches en gc
WO2025264836A1 (fr) 2024-06-18 2025-12-26 Illumina, Inc. Procédés pour augmenter la qualité de séquençage de régions riches en gc
WO2026006314A1 (fr) 2024-06-28 2026-01-02 Illumina, Inc. Marquage de régions cibles avant le séquençage de nucléotides
WO2026043885A1 (fr) 2024-08-20 2026-02-26 10X Genomics, Inc. Procédés et systèmes de préparation d'une bibliothèque d'acides nucléiques

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4237224A (en) * 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
US4321365A (en) * 1977-10-19 1982-03-23 Research Corporation Oligonucleotides useful as adaptors in DNA cloning, adapted DNA molecules, and methods of preparing adaptors and adapted molecules
US4293652A (en) * 1979-05-25 1981-10-06 Cetus Corporation Method for synthesizing DNA sequentially
GB2258950B (en) * 1982-05-11 1993-07-07 Secr Defence Integrated antenna device with resistive connection
US5118605A (en) * 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4775619A (en) * 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US5242794A (en) * 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0224126A3 (fr) * 1985-11-25 1989-02-01 The University of Calgary Oligodéoxynucléotides complémentaires liées par covalence utilisées comme "primer linkers" universels pour la détermination de la séquence d'acides nucléiques
GB8612087D0 (en) * 1986-05-19 1986-06-25 Ici Plc Hybridisation probes
US5202231A (en) * 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US4942124A (en) * 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
US5102785A (en) * 1987-09-28 1992-04-07 E. I. Du Pont De Nemours And Company Method of gene mapping
US5093245A (en) * 1988-01-26 1992-03-03 Applied Biosystems Labeling by simultaneous ligation and restriction
US5002867A (en) * 1988-04-25 1991-03-26 Macevicz Stephen C Nucleic acid sequence determination by multiple mixed oligonucleotide probes
US5114839A (en) * 1988-05-24 1992-05-19 Gesellschaft Fur Biotechnologische Forsching Mbh Process for dna sequencing using oligonucleotide bank
US5489507A (en) * 1988-11-30 1996-02-06 Perkin-Elmer Corporation DNA detection by color complementation
JPH02299598A (ja) * 1989-04-14 1990-12-11 Ro Inst For Molecular Genetics & Geneteic Res 微視的サイズの別個の粒子と結合している核酸試料中のごく短い配列の全部または一部のオリゴヌクレチドプローブとのハイブリダイゼーションによる決定法
US5126239A (en) * 1990-03-14 1992-06-30 E. I. Du Pont De Nemours And Company Process for detecting polymorphisms on the basis of nucleotide differences
CA2036946C (fr) * 1990-04-06 2001-10-16 Kenneth V. Deugau Molecules de liaison pour indexation
US5427911A (en) * 1990-05-01 1995-06-27 Yale University Coupled amplification and sequencing of DNA
US6004744A (en) * 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
GB9208733D0 (en) * 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5403708A (en) * 1992-07-06 1995-04-04 Brennan; Thomas M. Methods and compositions for determining the sequence of nucleic acids
GB9214873D0 (en) * 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations
DE4321198A1 (de) * 1993-06-25 1995-01-05 Henkel Kgaa Verwendung von N-Allylaminoaromaten in Oxidationsfärbemitteln
GB9401200D0 (en) * 1994-01-21 1994-03-16 Medical Res Council Sequencing of nucleic acids

Also Published As

Publication number Publication date
JP2005323607A (ja) 2005-11-24
GR3036314T3 (en) 2001-10-31
WO1995027080A3 (fr) 1995-11-16
DE69520917D1 (de) 2001-06-21
AU2379195A (en) 1995-10-23
US5856093A (en) 1999-01-05
DE69520917T2 (de) 2001-12-20
EP0703991B1 (fr) 2001-05-16
JP2006197944A (ja) 2006-08-03
ATE201238T1 (de) 2001-06-15
JP4250174B2 (ja) 2009-04-08
WO1995027080A2 (fr) 1995-10-12
JP3872812B2 (ja) 2007-01-24
PT703991E (pt) 2001-10-30
JPH08511174A (ja) 1996-11-26
CA2163662A1 (fr) 1995-10-12
US5599675A (en) 1997-02-04
US5552278A (en) 1996-09-03
AU685628B2 (en) 1998-01-22
ES2159635T3 (es) 2001-10-16
EP0703991A1 (fr) 1996-04-03

Similar Documents

Publication Publication Date Title
DK0703991T3 (da) DNA-sekventering ved trinvis ligering og spaltning
CA2218017A1 (fr) Sequencage d'adn par des extensions d'oligonucleotides paralleles
DE69432419D1 (de) Ein elektrochemiluminescenter nachweis von nukleinsäuren auf basis der selbsttragenden sequenzreplikation
DE60005309D1 (de) Methode zur sequenzierung von polynukleotiden
DK1159453T3 (da) Fremgangsmåde til direkte nukleinsyresekventering
ATE347615T1 (de) Nukleinsäuresammlung
DE69737450D1 (de) Sequenzierung durch ligation kodierter adapter
DE69917303D1 (de) Genomische multi-loci analyse mittels eines verbesserten verfahrens basierend auf zyklischer dna-sequenzierung
ATE306562T1 (de) Verfahren zur analyse von wiederkehrenden pcr- podukten, das auf der analyse von dns- schmelzkurven basiert
WO2003052099A3 (fr) Methodes de clonage et d'analyse de genes en parallele
DE69940890D1 (de) Ein verfahren zur analyse von polynukleotiden
GB2409457B (en) Method to generate or determine nucleic acid tags corresponding to the terminal ends of DNA molecules using sequence analysis of gene expression(terminal SAGE
TW329476B (en) Method for detecting contaminant DNA in a protein product and kits used for the same purpose
ATE211180T1 (de) Verfahren zur nicht-radioaktiven detektion von membran-gebundenen nukleinsäuren und testkit
Brenner DNA sequencing by stepwise ligation and cleavage
JPS57130998A (en) Human interferon-beta-gene
Luk'ianov et al. In vitro cloning of DNA fragments by one polymerase chain reaction
ES2057164T3 (es) Fragmentos de acido nucleico especificos del gen de la vilina humana y su utilizacion con fines diagnosticos.
KR960023084A (ko) 수두 바이러스 게놈 디엔에이(dna)의 비교정량적 피씨알(pcr) 방법
Subramanian et al. SEQUENCES OF SV40 DNA
JPH1094A5 (fr)
MX9704276A (es) Una quimiocina novedosa expresada en adenoides inflamadas, su produccion y usos.