DK0815200T3 - Værtscelle, der udtrykker reduceret niveau af en metalloprotease, og fremgangsmåder, der anvender værtscellen i proteinproduktion - Google Patents

Værtscelle, der udtrykker reduceret niveau af en metalloprotease, og fremgangsmåder, der anvender værtscellen i proteinproduktion

Info

Publication number
DK0815200T3
DK0815200T3 DK96905768T DK96905768T DK0815200T3 DK 0815200 T3 DK0815200 T3 DK 0815200T3 DK 96905768 T DK96905768 T DK 96905768T DK 96905768 T DK96905768 T DK 96905768T DK 0815200 T3 DK0815200 T3 DK 0815200T3
Authority
DK
Denmark
Prior art keywords
host cell
metalloprotease
methods
protein production
reduced level
Prior art date
Application number
DK96905768T
Other languages
English (en)
Inventor
Jan Lehmbeck
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK0815200T3 publication Critical patent/DK0815200T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK96905768T 1995-03-20 1996-03-20 Værtscelle, der udtrykker reduceret niveau af en metalloprotease, og fremgangsmåder, der anvender værtscellen i proteinproduktion DK0815200T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK28495 1995-03-20
PCT/DK1996/000111 WO1996029391A1 (en) 1995-03-20 1996-03-20 Host cell expressing reduced levels of a metalloprotease and methods using the host cell in protein production

Publications (1)

Publication Number Publication Date
DK0815200T3 true DK0815200T3 (da) 2007-06-11

Family

ID=8091831

Family Applications (1)

Application Number Title Priority Date Filing Date
DK96905768T DK0815200T3 (da) 1995-03-20 1996-03-20 Værtscelle, der udtrykker reduceret niveau af en metalloprotease, og fremgangsmåder, der anvender værtscellen i proteinproduktion

Country Status (10)

Country Link
US (2) US5861280A (da)
EP (1) EP0815200B1 (da)
JP (2) JP3974171B2 (da)
CN (2) CN1225550C (da)
AT (1) ATE353953T1 (da)
AU (1) AU4940096A (da)
DE (1) DE69636899T2 (da)
DK (1) DK0815200T3 (da)
ES (1) ES2281079T3 (da)
WO (1) WO1996029391A1 (da)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5776730A (en) * 1995-12-15 1998-07-07 University Of Hawaii Neurospora hosts for the production of recombinant proteins, and methods for producing same
AU4200897A (en) * 1996-09-19 1998-04-14 Novo Nordisk A/S Novel host cells and methods of producing proteins
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
WO2000020555A2 (en) 1998-10-06 2000-04-13 Aarl Inc Transformation system in the field of filamentous fungal hosts: in chrysosporium
US6015703A (en) * 1998-03-10 2000-01-18 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase
EP1078080A1 (en) * 1998-05-15 2001-02-28 Novo Nordisk Biotech, Inc. Methods for producing polypeptides in filamentous fungal mutant cells
AU6188599A (en) 1998-10-26 2000-05-15 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
US7063962B2 (en) 2001-07-20 2006-06-20 Novozymes A/S DNA sequences for regulating transcription
EP1546342A2 (en) * 2002-09-20 2005-06-29 Cym1P A/S Methods for increasing the production of a recombinant polypeptide from a host cell
US7169881B2 (en) * 2004-10-29 2007-01-30 Clariant (France) Aminoplast resin compositions
US8680252B2 (en) 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
WO2008085487A1 (en) * 2006-12-27 2008-07-17 Immersion Corporation Virtual detents through vibrotactile feedback
CA2736661A1 (en) 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
EP2800809B1 (en) 2012-01-05 2018-03-07 Glykos Finland Oy Protease deficient filamentous fungal cells and methods of use thereof
DK2852610T3 (da) 2012-05-23 2018-09-03 Glykos Finland Oy Produktion af fucosylerede glycoproteiner
DK3019602T3 (da) 2013-07-10 2018-11-12 Glykos Finland Oy Multiple protease-defekte filamentøse svampeceller og fremgangsmåder til anvendelse deraf
CN104711242B (zh) * 2013-12-12 2017-11-10 青岛蔚蓝生物集团有限公司 一种中性蛋白酶及其应用
CN104711200B (zh) * 2013-12-12 2018-01-16 青岛蔚蓝生物集团有限公司 一种中性蛋白酶生产菌株及其应用
AU2015293949B2 (en) 2014-07-21 2019-07-25 Teknologian Tutkimuskeskus Vtt Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
CN116710471A (zh) 2020-12-15 2023-09-05 诺维信公司 具有减少的细胞运动的突变的宿主细胞
WO2026004879A1 (ja) * 2024-06-26 2026-01-02 キッコーマン株式会社 ｎｐＩＩ遺伝子不活化株を用いた醤油の製造方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE248925T1 (de) * 1988-01-05 2003-09-15 Roche Diagnostics Gmbh Verfahren zur herstellung von proteinen oder proteinhaltigen genprodukten
AU614121B2 (en) * 1988-05-04 1991-08-22 Novartis Ag Improvements in the production of polypeptides
CA1333777C (en) * 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
JPH06506117A (ja) * 1991-04-01 1994-07-14 メルク　エンド　カンパニー　インコーポレーテッド ピキア（Ｐｉｃｈｉａ）蛋白質分解活性に影響する遺伝子およびその使用
IL102259A0 (en) * 1991-07-01 1993-01-14 Amgen Inc Isolation and characterization of a protease from streptomyces lividans

Also Published As

Publication number Publication date
CN1769423A (zh) 2006-05-10
DE69636899D1 (de) 2007-03-29
JP3974171B2 (ja) 2007-09-12
EP0815200A1 (en) 1998-01-07
CN1179178A (zh) 1998-04-15
CN1225550C (zh) 2005-11-02
ES2281079T3 (es) 2007-09-16
JPH11502111A (ja) 1999-02-23
US5861280A (en) 1999-01-19
ATE353953T1 (de) 2007-03-15
DE69636899T2 (de) 2007-10-31
JP2006204304A (ja) 2006-08-10
US5968774A (en) 1999-10-19
EP0815200B1 (en) 2007-02-14
AU4940096A (en) 1996-10-08
WO1996029391A1 (en) 1996-09-26

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