DK0904412T3 - Fremgangsmåde til polynucleotid-amplificering - Google Patents
Fremgangsmåde til polynucleotid-amplificeringInfo
- Publication number
- DK0904412T3 DK0904412T3 DK97952592T DK97952592T DK0904412T3 DK 0904412 T3 DK0904412 T3 DK 0904412T3 DK 97952592 T DK97952592 T DK 97952592T DK 97952592 T DK97952592 T DK 97952592T DK 0904412 T3 DK0904412 T3 DK 0904412T3
- Authority
- DK
- Denmark
- Prior art keywords
- polynucleotide sequence
- target polynucleotide
- oligonucleotide
- oligonucleotide primer
- along
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US3313796P | 1996-12-20 | 1996-12-20 | |
| US08/965,492 US6482590B1 (en) | 1996-12-20 | 1997-11-06 | Method for polynucleotide amplification |
| PCT/US1997/023706 WO1998028443A1 (fr) | 1996-12-20 | 1997-12-17 | Methode d'amplification des polynucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK0904412T3 true DK0904412T3 (da) | 2002-02-11 |
Family
ID=26709340
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK97952592T DK0904412T3 (da) | 1996-12-20 | 1997-12-17 | Fremgangsmåde til polynucleotid-amplificering |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6482590B1 (fr) |
| EP (1) | EP0904412B1 (fr) |
| JP (1) | JP2002503089A (fr) |
| AT (1) | ATE206766T1 (fr) |
| CA (1) | CA2246225A1 (fr) |
| DE (1) | DE69707284T2 (fr) |
| DK (1) | DK0904412T3 (fr) |
| ES (1) | ES2165634T3 (fr) |
| PT (1) | PT904412E (fr) |
| WO (1) | WO1998028443A1 (fr) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7070962B1 (en) * | 1996-02-01 | 2006-07-04 | Aventis Pharmaceuticals Inc. | Positive controls in polynucleotide amplification |
| ATE428801T1 (de) * | 1996-06-04 | 2009-05-15 | Univ Utah Res Found | Überwachung der hybridisierung während pcr |
| FR2779154B1 (fr) * | 1998-05-27 | 2002-07-12 | Bio Merieux | Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre |
| US6200757B1 (en) * | 1999-01-19 | 2001-03-13 | Dade Behring Inc. | Method for controlling the extension of an oligonucleotide |
| US6692918B2 (en) | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
| CA2384838C (fr) | 1999-09-13 | 2006-07-18 | Nugen Technologies, Inc. | Procedes et compositions utiles pour l'amplification isothermique lineaire de sequences de polynucleotides |
| US6274353B1 (en) | 1999-09-22 | 2001-08-14 | Genecopoeia, Inc. | Method and compositions for improved polynucleotide synthesis |
| WO2001029212A1 (fr) * | 1999-10-19 | 2001-04-26 | Enchira Biotechnology Corporation | Procede de genese de chimeres de genomes entiers ou de polynucleotides de grande taille |
| ATE455186T1 (de) | 2000-06-26 | 2010-01-15 | Nugen Technologies Inc | Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren |
| US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
| WO2002048402A2 (fr) | 2000-12-13 | 2002-06-20 | Nugen Technologies, Inc. | Methodes et compositions de generation de copies multiples de sequences d'acides nucleiques et methodes de detection correspondantes |
| KR20030082535A (ko) | 2001-03-09 | 2003-10-22 | 뉴젠 테크놀로지스 인코포레이티드 | Rna 서열의 증폭을 위한 방법 및 조성물 |
| CA2439074A1 (fr) | 2001-03-09 | 2002-09-19 | Nugen Technologies, Inc. | Methodes et compositions pour amplification de sequences d'arn |
| EP1275735A1 (fr) * | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Composition et méthode pour l'amplification des acides nucléiques à la facon 'hot start' |
| JP4567673B2 (ja) | 2003-04-01 | 2010-10-20 | エラジェン バイオサイエンシズ インコーポレイテッド | ポリメラーゼ阻害剤およびその使用方法 |
| JP2006523465A (ja) | 2003-04-14 | 2006-10-19 | ニューゲン テクノロジーズ, インコーポレイテッド | ランダムにプライミングされる複合プライマーを用いる大規模増幅 |
| US20070117114A1 (en) * | 2005-07-07 | 2007-05-24 | Quanta Biosciences, Inc. | Compositions and methods for increasing amplification efficiency |
| US7939258B2 (en) | 2005-09-07 | 2011-05-10 | Nugen Technologies, Inc. | Nucleic acid amplification procedure using RNA and DNA composite primers |
| DE102005047617A1 (de) * | 2005-10-05 | 2007-04-19 | Qiagen Gmbh | Polymerase-Kettenreaktions-Verfahren unter Einsatz einer DNA-Polymerase mit Proofreading-Eigenschaft |
| WO2007074894A1 (fr) * | 2005-12-28 | 2007-07-05 | School Corporation, Azabu Veterinary Medicine Educational Institution | Procédé spécifique et sensible pour amplifier une séquence cible |
| CN101517091B (zh) * | 2006-06-01 | 2013-02-13 | 垂林克生物技术公司 | 用于核酸扩增的化学修饰的寡核苷酸引物 |
| US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
| US8034568B2 (en) | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
| GB2470672B (en) | 2008-03-21 | 2012-09-12 | Nugen Technologies Inc | Methods of RNA amplification in the presence of DNA |
| US20100311144A1 (en) | 2009-06-05 | 2010-12-09 | Life Technologies Corporation | Mutant dna polymerases |
| EP2847353B1 (fr) | 2012-05-10 | 2022-01-19 | The General Hospital Corporation | Procédés pour déterminer une séquence nucléotidique |
| KR20210072137A (ko) | 2012-06-14 | 2021-06-16 | 라이프 테크놀로지스 코포레이션 | 폴리머라제 연쇄 반응 (pcr)을 위한 신규 조성물, 방법 및 키트 |
| JP7016215B2 (ja) | 2014-01-27 | 2022-02-04 | ザ ジェネラル ホスピタル コーポレイション | 配列決定のために核酸を調製する方法 |
| WO2017121836A1 (fr) | 2016-01-15 | 2017-07-20 | Thermo Fisher Scientific Baltics Uab | Mutants d'adn polymérases thermophiles |
| CA3037185A1 (fr) | 2016-09-15 | 2018-03-22 | ArcherDX, Inc. | Procedes de preparation d'echantillon d'acide nucleique |
| JP6997773B2 (ja) | 2016-09-15 | 2022-01-18 | アーチャーディーエックス, エルエルシー | 無細胞dnaの分析用の核酸サンプル調製の方法 |
| US11618891B2 (en) | 2017-06-26 | 2023-04-04 | Thermo Fisher Scientific Baltics Uab | Thermophilic DNA polymerase mutants |
| US10876148B2 (en) | 2018-11-14 | 2020-12-29 | Element Biosciences, Inc. | De novo surface preparation and uses thereof |
| US10704094B1 (en) | 2018-11-14 | 2020-07-07 | Element Biosciences, Inc. | Multipart reagents having increased avidity for polymerase binding |
| US10768173B1 (en) | 2019-09-06 | 2020-09-08 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
| WO2020118255A1 (fr) | 2018-12-07 | 2020-06-11 | Element Biosciences, Inc. | Dispositif de cellule d'écoulement et son utilisation |
| US11268091B2 (en) * | 2018-12-13 | 2022-03-08 | Dna Script Sas | Direct oligonucleotide synthesis on cells and biomolecules |
| CN113396326A (zh) | 2018-12-19 | 2021-09-14 | 核酸有限责任公司 | 用于分子诊断的设备和方法 |
| US11287422B2 (en) | 2019-09-23 | 2022-03-29 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
| EP4118230A4 (fr) | 2020-03-09 | 2024-04-24 | Nuclein, LLC | Appareil et procédés de diagnostic moléculaire |
| GB2629259B (en) | 2021-07-21 | 2025-05-28 | Element Biosciences Inc | Optical systems for nucleic acid sequencing and methods thereof |
| AU2023325064A1 (en) | 2022-08-15 | 2025-03-06 | Element Biosciences, Inc. | Spatially resolved surface capture of nucleic acids |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5008182A (en) | 1986-01-10 | 1991-04-16 | Cetus Corporation | Detection of AIDS associated virus by polymerase chain reaction |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| JP2609238B2 (ja) | 1985-05-02 | 1997-05-14 | ジエネテイツクス・インスチチユ−ト・インコ−ポレ−テツド | 標的ヌクレオチド配列の検定に有用な試薬複合体を作製するための方法および核酸構築物 |
| US4800159A (en) * | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US4994368A (en) | 1987-07-23 | 1991-02-19 | Syntex (U.S.A.) Inc. | Amplification method for polynucleotide assays |
| US5508178A (en) | 1989-01-19 | 1996-04-16 | Rose; Samuel | Nucleic acid amplification using single primer |
| US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
| US5766849A (en) * | 1989-07-11 | 1998-06-16 | Gen-Probe Incorporated | Methods of amplifying nucleic acids using promoter-containing primer sequence |
| US5218088A (en) * | 1989-11-02 | 1993-06-08 | Purdue Research Foundation | Process for preparing dithiophosphate oligonucleotide analogs via nucleoside thiophosphoramidite intermediates |
| US5573907A (en) | 1990-01-26 | 1996-11-12 | Abbott Laboratories | Detecting and amplifying target nucleic acids using exonucleolytic activity |
| DE69118930T2 (de) | 1990-01-26 | 1997-01-09 | Abbott Lab | Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion |
| EP0549107B1 (fr) | 1991-10-11 | 1997-12-29 | BEHRINGWERKE Aktiengesellschaft | Méthode pour produire un polynucléotide qui peut être utilisé pour des amplifications à amorce unique et utilisation d'oligonucléotides contenant le phosphorotioate comme amorces pour l'amplification d'acides nucléiques |
| ATE152180T1 (de) | 1992-07-31 | 1997-05-15 | Behringwerke Ag | Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden |
| WO1994003624A1 (fr) | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Procedes d'amplification isothermique de molecules d'acide nucleique |
| US5614389A (en) | 1992-08-04 | 1997-03-25 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US5733733A (en) | 1992-08-04 | 1998-03-31 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US5436143A (en) * | 1992-12-23 | 1995-07-25 | Hyman; Edward D. | Method for enzymatic synthesis of oligonucleotides |
| JPH07198A (ja) | 1993-04-21 | 1995-01-06 | Toyobo Co Ltd | 核酸増幅反応の増幅産物による汚染を防止する方法 |
| US5605796A (en) | 1994-07-19 | 1997-02-25 | Behringwerke Ag | Method for preventing amplification of nucleic acid contaminants in amplification mixtures using nuclease-receptor conjugates |
| ZA956776B (en) | 1994-08-25 | 1996-03-20 | Akzo Nobel Nv | A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay |
| US5763594A (en) * | 1994-09-02 | 1998-06-09 | Andrew C. Hiatt | 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds |
| US5753439A (en) | 1995-05-19 | 1998-05-19 | Trustees Of Boston University | Nucleic acid detection methods |
| CA2176193A1 (fr) | 1995-05-22 | 1996-11-23 | John Wesley Backus | Methodes de preamplification de la reaction en chaine de la polymerase a l'aide d'exonucleases, en presence d'amorces a base de phosphorothioate |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| US5747255A (en) | 1995-09-29 | 1998-05-05 | Lynx Therapeutics, Inc. | Polynucleotide detection by isothermal amplification using cleavable oligonucleotides |
| WO1997023646A1 (fr) * | 1995-12-22 | 1997-07-03 | Behringwerke Aktiengesellschaft | Detection de differences dans les acides nucleiques |
| JP4104657B2 (ja) | 1996-02-01 | 2008-06-18 | アベンティス・ファーマシューティカルズ・インコーポレイテッド | ポリヌクレオチド増幅の陽性コントロール |
| AU2320597A (en) * | 1996-03-19 | 1997-10-10 | Molecular Tool, Inc. | Method for determining the nucleotide sequence of a polynucleotide |
-
1997
- 1997-11-06 US US08/965,492 patent/US6482590B1/en not_active Expired - Fee Related
- 1997-12-17 ES ES97952592T patent/ES2165634T3/es not_active Expired - Lifetime
- 1997-12-17 JP JP52903598A patent/JP2002503089A/ja active Pending
- 1997-12-17 AT AT97952592T patent/ATE206766T1/de not_active IP Right Cessation
- 1997-12-17 PT PT97952592T patent/PT904412E/pt unknown
- 1997-12-17 WO PCT/US1997/023706 patent/WO1998028443A1/fr not_active Ceased
- 1997-12-17 DE DE69707284T patent/DE69707284T2/de not_active Revoked
- 1997-12-17 EP EP97952592A patent/EP0904412B1/fr not_active Revoked
- 1997-12-17 CA CA002246225A patent/CA2246225A1/fr not_active Abandoned
- 1997-12-17 DK DK97952592T patent/DK0904412T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| EP0904412B1 (fr) | 2001-10-10 |
| JP2002503089A (ja) | 2002-01-29 |
| DE69707284T2 (de) | 2002-10-17 |
| US6482590B1 (en) | 2002-11-19 |
| DE69707284D1 (de) | 2001-11-15 |
| EP0904412A1 (fr) | 1999-03-31 |
| PT904412E (pt) | 2002-04-29 |
| ATE206766T1 (de) | 2001-10-15 |
| WO1998028443A1 (fr) | 1998-07-02 |
| CA2246225A1 (fr) | 1998-07-02 |
| ES2165634T3 (es) | 2002-03-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| PT904412E (pt) | Metedo para amplificacao de polinucleotidos | |
| EP1130118A3 (fr) | Amplification de séquences d'acides nucléiques longues avec PCR | |
| IL102765A (en) | Reagents and methods to detect and determine mycobacterium nucleic acids | |
| WO1995003426A3 (fr) | Procede d'amplification d'acides nucleiques par transcription utilisant le deplacement, reactifs et necessaire pour la mise en ×uvre de ce procede | |
| AU6846698A (en) | Method of nucleic acid amplification | |
| EA200300991A1 (ru) | Продукт, содержащий иммобилизованную нуклеиновую кислоту, полученный способом с участием химерного олигонуклеотидного праймера, днк-полимеразы и эндонуклеазы | |
| EP0439182A3 (en) | Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions | |
| AR085952A2 (es) | Polinucleotidos cebadores | |
| WO2000070095A3 (fr) | Amplification isothermique homogene et detection d'acides nucleiques utilisant un oligonucleotide de commutation de matrice | |
| WO1997041260A3 (fr) | Amplification soustractive | |
| IL107935A0 (en) | Amplification of targeted segments of nucleic acid | |
| DK0676476T3 (da) | Isotermisk nukleinsyreamplificering ved strengdeplacering | |
| WO2002006533A3 (fr) | Amplification pcr à amorce unique de l'arn | |
| WO2003052133A3 (fr) | Analogues oligonucleotidiques comprenant des pseudo-nucleotides intercalants | |
| EP0822256A3 (fr) | Polymérase d'ADN thermostable modifiée et composition ayant une polymérase d'ADN pour l'amplification d'acides nucléiques | |
| WO1999049081A3 (fr) | Amplification d'acides nucleiques | |
| ATE361377T1 (de) | Leistungstarke verknüpfung von nukleinsäuresegmenten | |
| ATE476523T1 (de) | Verfahren zur genomamplifikation | |
| NO20000538L (no) | Forsterkning av spesifisiteten til nukleinsyreamplifisering ved bærer nukleinsyre | |
| DE69427876D1 (de) | Methode zur detektion einer gezielten nukleinsäure | |
| WO2003027258A3 (fr) | Procedes de production d'adn recombine comprenant l'utilisation d'un melange dntp contenant des nucleotides modifies | |
| KR102124058B1 (ko) | 폴리뉴클레오티드 및 그의 용도 | |
| WO1997028279A3 (fr) | Controles positifs utiles dans l'amplification de polynucleotides | |
| ATE286145T1 (de) | Multiplex-pcr zum nachweis von ehec-infektionen | |
| CA2201127A1 (fr) | Methode de differentiation genetiques varietes du houblon |