DK0909312T3 - Fremgangsmåde til fremstilling af polypeptider - Google Patents

Fremgangsmåde til fremstilling af polypeptider

Info

Publication number: DK0909312T3
Authority: DK; Denmark
Prior art keywords: yeast cells; glucagon; genetically modified; short chain; polypeptides
Prior art date: 1996-07-05

Application number

DK97928141T

Other languages

Danish (da)

English (en)

Inventor

Michi Egel-Mitani

Jakob Brandt

Knud Vad

Original Assignee

Novo Nordisk As

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1996-07-05

Filing date

1997-07-04

Publication date

2003-03-17

1997-07-04 Application filed by Novo Nordisk As filed Critical Novo Nordisk As

2003-03-17 Application granted granted Critical

2003-03-17 Publication of DK0909312T3 publication Critical patent/DK0909312T3/da

Links

229920001184 polypeptide Polymers 0.000 title abstract 4
102000004196 processed proteins & peptides Human genes 0.000 title abstract 4
108090000765 processed proteins & peptides Proteins 0.000 title abstract 4
238000000034 method Methods 0.000 title abstract 3
210000005253 yeast cell Anatomy 0.000 abstract 3
DDYAPMZTJAYBOF-ZMYDTDHYSA-N (3S)-4-[[(2S)-1-[[(2S)-1-[[(2S)-5-amino-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-4-amino-1-[[(2S,3R)-1-[[(2S)-6-amino-1-[[(2S)-1-[[(2S)-4-amino-1-[[(2S)-1-[[(2S)-4-amino-1-[[(2S)-4-amino-1-[[(2S,3S)-1-[[(1S)-1-carboxyethyl]amino]-3-methyl-1-oxopentan-2-yl]amino]-1,4-dioxobutan-2-yl]amino]-1,4-dioxobutan-2-yl]amino]-5-carbamimidamido-1-oxopentan-2-yl]amino]-1,4-dioxobutan-2-yl]amino]-5-carbamimidamido-1-oxopentan-2-yl]amino]-1-oxohexan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-1,4-dioxobutan-2-yl]amino]-4-methylsulfanyl-1-oxobutan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-3-(1H-indol-3-yl)-1-oxopropan-2-yl]amino]-1,5-dioxopentan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-1-oxo-3-phenylpropan-2-yl]amino]-3-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S,3R)-2-[[(2S)-2-[[(2S,3R)-2-[[2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-amino-3-(1H-imidazol-4-yl)propanoyl]amino]-3-hydroxypropanoyl]amino]-5-oxopentanoyl]amino]acetyl]amino]-3-hydroxybutanoyl]amino]-3-phenylpropanoyl]amino]-3-hydroxybutanoyl]amino]-3-hydroxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-3-hydroxypropanoyl]amino]hexanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-4-methylpentanoyl]amino]-3-carboxypropanoyl]amino]-3-hydroxypropanoyl]amino]-5-carbamimidamidopentanoyl]amino]-5-carbamimidamidopentanoyl]amino]propanoyl]amino]-5-oxopentanoyl]amino]-4-oxobutanoic acid Chemical class [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(O)=O DDYAPMZTJAYBOF-ZMYDTDHYSA-N 0.000 abstract 1
102000051325 Glucagon Human genes 0.000 abstract 1
108060003199 Glucagon Proteins 0.000 abstract 1
108010088406 Glucagon-Like Peptides Proteins 0.000 abstract 1
108091005804 Peptidases Proteins 0.000 abstract 1
239000004365 Protease Substances 0.000 abstract 1
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
101100319895 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YAP3 gene Proteins 0.000 abstract 1
101100160515 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YPS1 gene Proteins 0.000 abstract 1
125000000539 amino acid group Chemical group 0.000 abstract 1
MASNOZXLGMXCHN-ZLPAWPGGSA-N glucagon Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 MASNOZXLGMXCHN-ZLPAWPGGSA-N 0.000 abstract 1
229960004666 glucagon Drugs 0.000 abstract 1
238000004519 manufacturing process Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/57509—Corticotropin releasing factor [CRF] (Urotensin)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/605—Glucagons
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Gastroenterology & Hepatology (AREA)
Toxicology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Medicinal Chemistry (AREA)
Biotechnology (AREA)
Mycology (AREA)
Biomedical Technology (AREA)
Endocrinology (AREA)
General Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Microbiology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Peptides Or Proteins (AREA)

DK97928141T 1996-07-05 1997-07-04 Fremgangsmåde til fremstilling af polypeptider DK0909312T3 (da)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DK74996		1996-07-05
PCT/DK1997/000298 WO1998001535A1 (en)	1996-07-05	1997-07-04	Method for the production of polypeptides

Publications (1)

Publication Number	Publication Date
DK0909312T3 true DK0909312T3 (da)	2003-03-17

Family

ID=8097103

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK97928141T DK0909312T3 (da)	1996-07-05	1997-07-04	Fremgangsmåde til fremstilling af polypeptider

Country Status (11)

Country	Link
EP (1)	EP0909312B1 (de)
JP (1)	JP3998717B2 (de)
CN (1)	CN1150313C (de)
AT (1)	ATE229068T1 (de)
AU (1)	AU3255497A (de)
CA (1)	CA2258307C (de)
DE (1)	DE69717629T2 (de)
DK (1)	DK0909312T3 (de)
ES (1)	ES2187782T3 (de)
IL (1)	IL127583A0 (de)
WO (1)	WO1998001535A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5891669A (en) *	1997-03-17	1999-04-06	Novo Nordisk A/S, Novoalle,	Methods for producing polypeptides in respiratory-deficient cells
WO1999037758A2 (en) *	1998-01-27	1999-07-29	The Board Of Regents Of The University And Community College System Of Nevada On Behalf Of The University Of Nevada-Reno	Expression of proteolytically-sensitive peptides
US6190883B1 (en)	1998-09-09	2001-02-20	Novo Nordisk A/S	Method for the production of heterologous polypeptides in transformed yeast cells
US7371721B2 (en)	2000-09-18	2008-05-13	Sanos Bioscience A/S	Use of GLP-2 and related compounds for the treatment, prevention, diagnosis, and prognosis of bone-related disorders and calcium homeostasis related syndromes
WO2002022151A2 (en)	2000-09-18	2002-03-21	Osteometer Bio Tech A/S	Use of glp-1 and flp-2 peptides for treatment of bone disorders
US7595172B2 (en)	2001-07-24	2009-09-29	Novo Nordisk A/S	Method for making acylated polypeptides
KR100496758B1 (ko)	2002-06-29	2005-06-22	한국생명공학연구원	한세눌라 폴리모르파 앱신 유전자 결손 변이주 및 이를이용한 재조합 단백질 생산 방법
EP1687428A1 (de)	2003-11-14	2006-08-09	Novo Nordisk A/S	Verfahren zur herstellung von acyliertem insulin
CN102660614A (zh)	2005-08-16	2012-09-12	诺沃-诺迪斯克有限公司	制备成熟胰岛素多肽的方法
US8114632B2 (en)	2006-06-21	2012-02-14	Biocon Limited	Method of producing biologically active polypeptide having insulinotropic activity
US8518668B2 (en)	2006-09-27	2013-08-27	Novo Nordisk A/S	Method for making maturated insulin polypeptides in a fungal cell
EP2134853B1 (de)	2007-04-03	2018-07-18	Oxyrane UK Limited	Glycosylierung von molekülen
KR101005821B1 (ko) *	2007-12-28	2011-01-05	(주)바이오버드	배트록소빈의 변이 뉴클레오타이드 서열, α－인자 분비 시그널 서열 변이체 및 이를 이용한 재조합 단백질의 제조방법및 이를 이용한 배트록소빈의 제조방법
US9598682B2 (en)	2009-09-29	2017-03-21	Vib Vzw	Hydrolysis of mannose-1-phospho-6-mannose linkage to phospho-6-mannose
JP2013511272A (ja)	2009-11-19	2013-04-04	オキシレインユーケーリミテッド	哺乳類様複合ｎ−グリカンを生成する酵母株
EP2504355B1 (de) *	2009-11-25	2015-07-22	Novo Nordisk A/S	Verfahren zur herstellung von polypeptiden
WO2012042386A2 (en)	2010-09-29	2012-04-05	Oxyrane Uk Limited	Mannosidases capable of uncapping mannose-1-phospho-6-mannose linkages and demannosylating phosphorylated n-glycans and methods of facilitating mammalian cellular uptake of glycoproteins
SG189110A1 (en)	2010-09-29	2013-05-31	Oxyrane Uk Ltd	De-mannosylation of phosphorylated n-glycans
CN104136622A (zh) *	2011-12-30	2014-11-05	奥克西雷恩英国有限公司	用于降低重组蛋白的降解的方法和材料
JP6194324B2 (ja)	2012-03-15	2017-09-06	オキシレインユーケーリミテッド	ポンペ病の処置のための方法および材料
CA2903739A1 (en) *	2013-03-06	2014-09-12	Glaxosmithkline Llc	Host cells and methods of use
CN106029688A (zh) *	2014-02-28	2016-10-12	诺和诺德股份有限公司	交配因子α原肽变体
US12152062B2 (en) *	2019-02-24	2024-11-26	Oncosimis Biotech Privated Limited	Method for continuous production of recombinant GLP-1 peptide by bacteria
GB202301988D0 (en) *	2023-02-13	2023-03-29	Phenotypeca Ltd	Breeding process
CN120272338B (zh) *	2025-06-04	2025-08-26	上海欣瑞特生物医药技术有限公司	一种用于制备重组牛白蛋白的重组工程菌及其应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB9404270D0 (en) *	1994-03-05	1994-04-20	Delta Biotechnology Ltd	Yeast strains and modified albumins

1997
- 1997-07-04 DK DK97928141T patent/DK0909312T3/da active
- 1997-07-04 DE DE69717629T patent/DE69717629T2/de not_active Expired - Lifetime
- 1997-07-04 ES ES97928141T patent/ES2187782T3/es not_active Expired - Lifetime
- 1997-07-04 EP EP97928141A patent/EP0909312B1/de not_active Expired - Lifetime
- 1997-07-04 WO PCT/DK1997/000298 patent/WO1998001535A1/en not_active Ceased
- 1997-07-04 CN CNB971961824A patent/CN1150313C/zh not_active Expired - Fee Related
- 1997-07-04 AT AT97928141T patent/ATE229068T1/de not_active IP Right Cessation
- 1997-07-04 CA CA002258307A patent/CA2258307C/en not_active Expired - Fee Related
- 1997-07-04 IL IL12758397A patent/IL127583A0/xx not_active IP Right Cessation
- 1997-07-04 AU AU32554/97A patent/AU3255497A/en not_active Abandoned
- 1997-07-04 JP JP50467898A patent/JP3998717B2/ja not_active Expired - Fee Related

Also Published As

Publication number	Publication date
IL127583A0 (en)	1999-10-28
CA2258307A1 (en)	1998-01-15
JP2000513941A (ja)	2000-10-24
EP0909312B1 (de)	2002-12-04
DE69717629D1 (de)	2003-01-16
WO1998001535A1 (en)	1998-01-15
ATE229068T1 (de)	2002-12-15
CN1150313C (zh)	2004-05-19
DE69717629T2 (de)	2003-09-11
CA2258307C (en)	2008-02-12
CN1225126A (zh)	1999-08-04
ES2187782T3 (es)	2003-06-16
EP0909312A1 (de)	1999-04-21
AU3255497A (en)	1998-02-02
JP3998717B2 (ja)	2007-10-31

Publication	Publication Date	Title
ATE229068T1 (de)	2002-12-15	Verfahren zur produktion von polypeptiden
DE68921287D1 (de)	1995-03-30	Nukleotidesequenzen, die ein Protein mit Ureareaktivität kodieren.
DE69034091D1 (de)	2003-09-04	Herstellung von Hämoglobin und Analogen davon in Nicht-Erythrozytzellen
ES2078518T3 (es)	1995-12-16	Mutagenesis por desplazamiento completo.
CA2331337A1 (en)	1999-12-29	Site specific protein modification by mutagenesis
CS9100241A2 (en)	1991-08-13	Method of composite proteins' enzymic splitting
DE60233722D1 (de)	2009-10-29	Insulinvorstufen und verfahren zu deren herstellung
GB2301365A (en)	1996-12-04	Yeast strains and modified albumins
DK1150999T3 (da)	2006-10-30	Cyclosporinderivater og fremgangsmåde til fremstilling deraf
CA2346257A1 (en)	2000-04-20	Method for obtaining active .beta.-ngf
EP0297362A3 (de)	1990-05-30	Human-Aprotinin, dessen Lys-Rest in Position 15 gegen einen anderen protogenen Aminosäurerest ausgetauscht ist
KR900012626A (ko)	1990-09-01	하이브리드 단백질, 이의 생산방법 및 이를 함유하는 약제학적 조성물
WO1998051780A3 (en)	1999-03-25	Method for increasing yields or recombinant proteins
DE3852105D1 (de)	1994-12-15	Verfahren zur herstellung von aprotinin und aprotinin-homologen in hefe.
ATE313632T1 (de)	2006-01-15	Durch pflanzen hergestellte rekombinante preduodenale lipase und abgeleitete polypeptide, verfahren zur herstellung und ihre verwendung
DK0408764T3 (da)	1995-07-17	Fremgangsmåde til fremstilling af alfa-amiderede peptider
KR890008166A (ko)	1989-07-10	융합 단백질의 선택적 분해 방법
FI913405A0 (fi)	1991-07-15	In vitro preparering av fusionsproteiner.
ATE123056T1 (de)	1995-06-15	Ancrod-proteine, ihre herstellung und verwendung.
ATE171711T1 (de)	1998-10-15	Cyclohexapeptide und deren mischungen, verfahren zur herstellung sowie deren verwendung
ATE103341T1 (de)	1994-04-15	Antikoerper gegen hochkonservierte aminosaeuresequenzen von immunogenen substanzen, verfahren zur herstellung dieser antikoerper, sowie deren verwendung in immunoassays.
EP0945512A3 (de)	2002-01-16	Serinproteasen
ATE130872T1 (de)	1995-12-15	Ancrod ähnliche proteine, ihre herstellung und verwendung.
DE3677851D1 (de)	1991-04-11	Verfahren zur steigerung der produktion von rekombinantem protein in hefearten der gattung saccharomyces.