ES2078518T3 - Mutagenesis por desplazamiento completo. - Google Patents

Mutagenesis por desplazamiento completo.

Info

Publication number
ES2078518T3
ES2078518T3 ES91908402T ES91908402T ES2078518T3 ES 2078518 T3 ES2078518 T3 ES 2078518T3 ES 91908402 T ES91908402 T ES 91908402T ES 91908402 T ES91908402 T ES 91908402T ES 2078518 T3 ES2078518 T3 ES 2078518T3
Authority
ES
Spain
Prior art keywords
antibodies
libraries
amino acids
mutagenesis
mutants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91908402T
Other languages
English (en)
Inventor
Roberto Crea
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of ES2078518T3 publication Critical patent/ES2078518T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0002—Antibodies with enzymatic activity, e.g. abzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Arc Welding Control (AREA)
  • Arc Welding In General (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Battery Electrode And Active Subsutance (AREA)
  • Materials Applied To Surfaces To Minimize Adherence Of Mist Or Water (AREA)
  • Confectionery (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)

Abstract

UN METODO DE MUTAGENESIS POR MEDIO DEL CUAL SE INTRODUCE UN AMINOACIDO PREDETERMINADO EN TODAS Y CADA UNA DE LAS POSICIONES DE UN CONJUNTO SELECCIONADO DE POSICIONES DE UNA ZONA PRESELECCIONADA (O VARIAS ZONAS DISTINTAS) DE UNA PROTEINA PARA PRODUCIR UNA BIBLIOTECA DE MUTANTES. EL METODO SE BASA EN LA PREMISA DE QUE CIERTOS AMINOACIDOS REPRESENTAN UN PAPEL CRUCIAL EN LA ESTRUCTURA Y LA FUNCION DE LAS PROTEINAS. SE PUEDEN GENERAR LAS BIBLIOTECAS PARA QUE CONTENGAN UNA PROPORCION ALTA DE LOS MUTANTES DESEADOS Y PARA QUE TENGAN UN TAMAÑO RAZONABLE PARA SU CLASIFICACION. SE PUEDEN UTILIZAR ESTAS BIBLIOTECAS PARA ESTUDIAR EL PAPEL DE LOS AMINOACIDOS ESPECIFICOS EN LA ESTRUCTURA Y LA FUNCION DE LAS PROTEINAS Y PARA ELABORAR PROTEINAS Y POLIPEPTIDOS MEJORADOS O NUEVOS TALES COMO ENZIMAS, ANTICUERPOS, ANTICUERPOS DE CADENA SIMPLE Y ANTICUERPOS CATALITICOS.
ES91908402T 1990-04-05 1991-04-05 Mutagenesis por desplazamiento completo. Expired - Lifetime ES2078518T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US50531490A 1990-04-05 1990-04-05

Publications (1)

Publication Number Publication Date
ES2078518T3 true ES2078518T3 (es) 1995-12-16

Family

ID=24009833

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91908402T Expired - Lifetime ES2078518T3 (es) 1990-04-05 1991-04-05 Mutagenesis por desplazamiento completo.

Country Status (8)

Country Link
US (3) US5798208A (es)
EP (1) EP0527809B1 (es)
JP (2) JP4251406B2 (es)
AT (1) ATE126535T1 (es)
CA (1) CA2079802C (es)
DE (1) DE69112207T2 (es)
ES (1) ES2078518T3 (es)
WO (1) WO1991015581A1 (es)

Families Citing this family (124)

* Cited by examiner, † Cited by third party
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US5830650A (en) 1998-11-03
US5798208A (en) 1998-08-25
AU653152B2 (en) 1994-09-22
US6649340B1 (en) 2003-11-18
DE69112207D1 (en) 1995-09-21
ATE126535T1 (de) 1995-09-15
JP4275922B2 (ja) 2009-06-10
EP0527809A1 (en) 1993-02-24
CA2079802C (en) 2001-09-18
EP0527809B1 (en) 1995-08-16
JP2003135084A (ja) 2003-05-13
JPH05506580A (ja) 1993-09-30
AU7741891A (en) 1991-10-30
CA2079802A1 (en) 1991-10-06
WO1991015581A1 (en) 1991-10-17
DE69112207T2 (de) 1996-03-28
JP4251406B2 (ja) 2009-04-08

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