ATE229068T1 - Verfahren zur produktion von polypeptiden - Google Patents

Verfahren zur produktion von polypeptiden

Info

Publication number: ATE229068T1
Authority: AT; Austria
Prior art keywords: yeast cells; glucagon; genetically modified; short chain; polypeptides
Prior art date: 1996-07-05

Application number

AT97928141T

Other languages

English (en)

Inventor

Michi Egel-Mitani

Jakob Brandt

Knud Vad

Original Assignee

Novo Nordisk As

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1996-07-05

Filing date

1997-07-04

Publication date

2002-12-15

1997-07-04 Application filed by Novo Nordisk As filed Critical Novo Nordisk As

2002-12-15 Application granted granted Critical

2002-12-15 Publication of ATE229068T1 publication Critical patent/ATE229068T1/de

Links

229920001184 polypeptide Polymers 0.000 title abstract 4
102000004196 processed proteins & peptides Human genes 0.000 title abstract 4
108090000765 processed proteins & peptides Proteins 0.000 title abstract 4
238000004519 manufacturing process Methods 0.000 title abstract 2
210000005253 yeast cell Anatomy 0.000 abstract 3
238000000034 method Methods 0.000 abstract 2
DDYAPMZTJAYBOF-ZMYDTDHYSA-N (3S)-4-[[(2S)-1-[[(2S)-1-[[(2S)-5-amino-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-4-amino-1-[[(2S,3R)-1-[[(2S)-6-amino-1-[[(2S)-1-[[(2S)-4-amino-1-[[(2S)-1-[[(2S)-4-amino-1-[[(2S)-4-amino-1-[[(2S,3S)-1-[[(1S)-1-carboxyethyl]amino]-3-methyl-1-oxopentan-2-yl]amino]-1,4-dioxobutan-2-yl]amino]-1,4-dioxobutan-2-yl]amino]-5-carbamimidamido-1-oxopentan-2-yl]amino]-1,4-dioxobutan-2-yl]amino]-5-carbamimidamido-1-oxopentan-2-yl]amino]-1-oxohexan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-1,4-dioxobutan-2-yl]amino]-4-methylsulfanyl-1-oxobutan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-3-(1H-indol-3-yl)-1-oxopropan-2-yl]amino]-1,5-dioxopentan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-1-oxo-3-phenylpropan-2-yl]amino]-3-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S,3R)-2-[[(2S)-2-[[(2S,3R)-2-[[2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-amino-3-(1H-imidazol-4-yl)propanoyl]amino]-3-hydroxypropanoyl]amino]-5-oxopentanoyl]amino]acetyl]amino]-3-hydroxybutanoyl]amino]-3-phenylpropanoyl]amino]-3-hydroxybutanoyl]amino]-3-hydroxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-3-hydroxypropanoyl]amino]hexanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-4-methylpentanoyl]amino]-3-carboxypropanoyl]amino]-3-hydroxypropanoyl]amino]-5-carbamimidamidopentanoyl]amino]-5-carbamimidamidopentanoyl]amino]propanoyl]amino]-5-oxopentanoyl]amino]-4-oxobutanoic acid Chemical class [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(O)=O DDYAPMZTJAYBOF-ZMYDTDHYSA-N 0.000 abstract 1
102000051325 Glucagon Human genes 0.000 abstract 1
108060003199 Glucagon Proteins 0.000 abstract 1
108010088406 Glucagon-Like Peptides Proteins 0.000 abstract 1
108091005804 Peptidases Proteins 0.000 abstract 1
239000004365 Protease Substances 0.000 abstract 1
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
101100319895 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YAP3 gene Proteins 0.000 abstract 1
101100160515 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YPS1 gene Proteins 0.000 abstract 1
125000000539 amino acid group Chemical group 0.000 abstract 1
MASNOZXLGMXCHN-ZLPAWPGGSA-N glucagon Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 MASNOZXLGMXCHN-ZLPAWPGGSA-N 0.000 abstract 1
229960004666 glucagon Drugs 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/57509—Corticotropin releasing factor [CRF] (Urotensin)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/605—Glucagons
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Gastroenterology & Hepatology (AREA)
Toxicology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Medicinal Chemistry (AREA)
Biotechnology (AREA)
Mycology (AREA)
Biomedical Technology (AREA)
Endocrinology (AREA)
General Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Microbiology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Peptides Or Proteins (AREA)

AT97928141T 1996-07-05 1997-07-04 Verfahren zur produktion von polypeptiden ATE229068T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DK74996		1996-07-05
PCT/DK1997/000298 WO1998001535A1 (en)	1996-07-05	1997-07-04	Method for the production of polypeptides

Publications (1)

Publication Number	Publication Date
ATE229068T1 true ATE229068T1 (de)	2002-12-15

Family

ID=8097103

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT97928141T ATE229068T1 (de)	1996-07-05	1997-07-04	Verfahren zur produktion von polypeptiden

Country Status (11)

Country	Link
EP (1)	EP0909312B1 (de)
JP (1)	JP3998717B2 (de)
CN (1)	CN1150313C (de)
AT (1)	ATE229068T1 (de)
AU (1)	AU3255497A (de)
CA (1)	CA2258307C (de)
DE (1)	DE69717629T2 (de)
DK (1)	DK0909312T3 (de)
ES (1)	ES2187782T3 (de)
IL (1)	IL127583A0 (de)
WO (1)	WO1998001535A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5891669A (en) *	1997-03-17	1999-04-06	Novo Nordisk A/S, Novoalle,	Methods for producing polypeptides in respiratory-deficient cells
AU2241699A (en) *	1998-01-27	1999-08-09	Board Of Regents Of The University And Community College System Of Nevada, The	Expression of proteolytically-sensitive peptides
US6190883B1 (en)	1998-09-09	2001-02-20	Novo Nordisk A/S	Method for the production of heterologous polypeptides in transformed yeast cells
PT1326630E (pt)	2000-09-18	2008-09-02	Sanos Bioscience As	Utilização de péptidos glp-2
US7371721B2 (en)	2000-09-18	2008-05-13	Sanos Bioscience A/S	Use of GLP-2 and related compounds for the treatment, prevention, diagnosis, and prognosis of bone-related disorders and calcium homeostasis related syndromes
US7595172B2 (en)	2001-07-24	2009-09-29	Novo Nordisk A/S	Method for making acylated polypeptides
AU2003237061A1 (en) *	2002-06-29	2004-01-19	Bioholdings Co., Ltd	Hansenula polymorpha yapsin deficient mutant strain and process for the preparation of recombinant proteins using the same
EP1687428A1 (de)	2003-11-14	2006-08-09	Novo Nordisk A/S	Verfahren zur herstellung von acyliertem insulin
CN101243190B (zh)	2005-08-16	2015-05-13	诺沃-诺迪斯克有限公司	制备成熟胰岛素多肽的方法
CN101501209B (zh)	2006-06-21	2013-06-05	百奥勤有限公司	具有促胰岛素活性的生物活性多肽的制备方法
US8518668B2 (en)	2006-09-27	2013-08-27	Novo Nordisk A/S	Method for making maturated insulin polypeptides in a fungal cell
CA2887752C (en)	2007-04-03	2020-03-24	Vib Vzw	Glycosylation of molecules
KR101005821B1 (ko) *	2007-12-28	2011-01-05	(주)바이오버드	배트록소빈의 변이 뉴클레오타이드 서열, α－인자 분비 시그널 서열 변이체 및 이를 이용한 재조합 단백질의 제조방법및 이를 이용한 배트록소빈의 제조방법
CN102712914A (zh)	2009-09-29	2012-10-03	根特大学	水解甘露糖-1-磷酸-6-甘露糖连接为磷酸-6-甘露糖
DK2501805T3 (en)	2009-11-19	2019-04-15	Oxyrane Uk Ltd	Yeast strains producing mammalian-like complex N-Glycans
CN102639559B (zh) *	2009-11-25	2015-04-29	诺沃—诺迪斯克有限公司	用于制备多肽的方法
BR112013007616B1 (pt)	2010-09-29	2023-01-24	Oxyrane Uk Limited	Métodos para retirar o cap ("uncapping") de porções de manose-1- fosfo-6-manose e desmanosilar n-glicanos fosforilados em uma glicoproteína, para direcionar uma glicoproteína para o interior de uma célula de mamífero, e para converter uma glicoproteína de uma primeira forma que não se liga substancialmente a um receptor de manose-6-fosfato em uma segunda forma que se liga substancialmente a um receptor de manose-6-fosfato em uma célula de mamífero
WO2012042387A2 (en)	2010-09-29	2012-04-05	Oxyrane Uk Limited	De-mannosylation of phosphorylated n-glycans
EP2798074A1 (de) *	2011-12-30	2014-11-05	Oxyrane UK Limited	Verfahren und materialien zur reduktion der degradation von rekombinanten proteinen
EP3628326B1 (de)	2012-03-15	2024-02-28	Oxyrane UK Limited	Verfahren und materialien zur behandlung von morbus pompe
EP2964666B1 (de) *	2013-03-06	2020-11-18	GlaxoSmithKline LLC	Wirtszellen und verfahren zur verwendung
CN120060321A (zh) *	2014-02-28	2025-05-30	诺和诺德股份有限公司	交配因子α原肽变体
JP7487951B2 (ja) *	2019-02-24	2024-05-21	オンコシミスバイオテックプライベートリミテッド	細菌による組換えｇｌｐ－１ペプチドの連続生産方法
GB202301988D0 (en) *	2023-02-13	2023-03-29	Phenotypeca Ltd	Breeding process
CN120272338B (zh) *	2025-06-04	2025-08-26	上海欣瑞特生物医药技术有限公司	一种用于制备重组牛白蛋白的重组工程菌及其应用

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GB9404270D0 (en) *	1994-03-05	1994-04-20	Delta Biotechnology Ltd	Yeast strains and modified albumins

1997
- 1997-07-04 JP JP50467898A patent/JP3998717B2/ja not_active Expired - Fee Related
- 1997-07-04 EP EP97928141A patent/EP0909312B1/de not_active Expired - Lifetime
- 1997-07-04 CN CNB971961824A patent/CN1150313C/zh not_active Expired - Fee Related
- 1997-07-04 WO PCT/DK1997/000298 patent/WO1998001535A1/en not_active Ceased
- 1997-07-04 AU AU32554/97A patent/AU3255497A/en not_active Abandoned
- 1997-07-04 IL IL12758397A patent/IL127583A0/xx not_active IP Right Cessation
- 1997-07-04 AT AT97928141T patent/ATE229068T1/de not_active IP Right Cessation
- 1997-07-04 CA CA002258307A patent/CA2258307C/en not_active Expired - Fee Related
- 1997-07-04 DE DE69717629T patent/DE69717629T2/de not_active Expired - Lifetime
- 1997-07-04 DK DK97928141T patent/DK0909312T3/da active
- 1997-07-04 ES ES97928141T patent/ES2187782T3/es not_active Expired - Lifetime

Also Published As

Publication number	Publication date
IL127583A0 (en)	1999-10-28
CN1225126A (zh)	1999-08-04
DK0909312T3 (da)	2003-03-17
CA2258307A1 (en)	1998-01-15
EP0909312B1 (de)	2002-12-04
DE69717629T2 (de)	2003-09-11
WO1998001535A1 (en)	1998-01-15
DE69717629D1 (de)	2003-01-16
JP3998717B2 (ja)	2007-10-31
ES2187782T3 (es)	2003-06-16
CN1150313C (zh)	2004-05-19
EP0909312A1 (de)	1999-04-21
CA2258307C (en)	2008-02-12
AU3255497A (en)	1998-02-02
JP2000513941A (ja)	2000-10-24

Legal Events

Date	Code	Title	Description
2003-06-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties

Publication	Publication Date	Title
DE69717629D1 (de)	2003-01-16	Verfahren zur produktion von polypeptiden
DE68929115D1 (de)	2000-01-20	Verfahren zur Herstellung von menschlichem Serumalbumin in Hefe
DE69034091D1 (de)	2003-09-04	Herstellung von Hämoglobin und Analogen davon in Nicht-Erythrozytzellen
CA2155185A1 (en)	1994-08-18	Totally synthetic affinity reagents
CA2331337A1 (en)	1999-12-29	Site specific protein modification by mutagenesis
DE68929510D1 (de)	2004-02-12	Nukleotidsequenzen, die für ein Protein mit Ureaseaktivität kodieren
DE60331226D1 (de)	2010-03-25	Verfahren und dna-konstrukte zur produktion von polypeptiden mit hoher ausbeute
ATE443082T1 (de)	2009-10-15	Insulinvorstufen und verfahren zu deren herstellung
ATE212059T1 (de)	2002-02-15	Methoden zur identifizierung heterofunktionaler fusionsproteine
ATE105859T1 (de)	1994-06-15	Proteolytisches enzym, spezifisch für menschlichen immunmangel-virus, verfahren zu seiner herstellung und renaturierung.
EP0297362A3 (de)	1990-05-30	Human-Aprotinin, dessen Lys-Rest in Position 15 gegen einen anderen protogenen Aminosäurerest ausgetauscht ist
DE3852105D1 (de)	1994-12-15	Verfahren zur herstellung von aprotinin und aprotinin-homologen in hefe.
DE3875359D1 (de)	1992-11-26	Dipeptide, verfahren zur herstellung und verwendung in der bestimmung von proteasen.
WO1998051780A3 (en)	1999-03-25	Method for increasing yields or recombinant proteins
ATE313632T1 (de)	2006-01-15	Durch pflanzen hergestellte rekombinante preduodenale lipase und abgeleitete polypeptide, verfahren zur herstellung und ihre verwendung
DE69019077D1 (de)	1995-06-08	Verfahren zur herstellung von c-terminalen alpha-amidierten peptiden.
DE69403654D1 (de)	1997-07-10	Ein verfahren zur erhöhung der produktion von biologisch aktiven rekombinanten proteinen
WO1998019160A3 (de)	1998-06-25	VERFAHREN ZUR INDIREKTEN BESTIMMUNG DER TYP I INTERFERON-PRODUKTION IN VIVO MITTELS ANTIKÖRPER GEGEN HUMANE Mx-PROTEINE SOWIE ANTIKÖRPER HIERZU
KR890008166A (ko)	1989-07-10	융합 단백질의 선택적 분해 방법
ATE141645T1 (de)	1996-09-15	In vitro-behandlung von fusionsproteinen
DE58901435D1 (de)	1992-06-17	Verfahren zur herstellung von dipeptiden mit n-terminalen nichtproteinogenen aminosaeuren.
AU637589B2 (en)	1993-06-03	Ancrod proteins, their production and use
ATE103341T1 (de)	1994-04-15	Antikoerper gegen hochkonservierte aminosaeuresequenzen von immunogenen substanzen, verfahren zur herstellung dieser antikoerper, sowie deren verwendung in immunoassays.
DE59503776D1 (de)	1998-11-05	Cyclohexapeptide und deren Mischungen, Verfahren zur Herstellung sowie deren Verwendung
DE3781254D1 (de)	1992-09-24	Verfahren zur herstellung von eiweisshydrolysaten, loeslich in einem saeuremilieu und die erhaltenen hydrolysate.