DK1017855T3 - Fremgangsmåde til syntetisering af polynucleotider ved ligering af flere oligomerer - Google Patents

Fremgangsmåde til syntetisering af polynucleotider ved ligering af flere oligomerer

Info

Publication number
DK1017855T3
DK1017855T3 DK99937170T DK99937170T DK1017855T3 DK 1017855 T3 DK1017855 T3 DK 1017855T3 DK 99937170 T DK99937170 T DK 99937170T DK 99937170 T DK99937170 T DK 99937170T DK 1017855 T3 DK1017855 T3 DK 1017855T3
Authority
DK
Denmark
Prior art keywords
oligomers
ligation
synthesis
synthesizing polynucleotides
methods
Prior art date
Application number
DK99937170T
Other languages
Danish (da)
English (en)
Inventor
Hashem Akhavan-Tafti
Original Assignee
Lumigen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/121,887 external-priority patent/US5998175A/en
Application filed by Lumigen Inc filed Critical Lumigen Inc
Application granted granted Critical
Publication of DK1017855T3 publication Critical patent/DK1017855T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DK99937170T 1998-07-24 1999-07-22 Fremgangsmåde til syntetisering af polynucleotider ved ligering af flere oligomerer DK1017855T3 (da)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US09/121,887 US5998175A (en) 1998-07-24 1998-07-24 Methods of synthesizing and amplifying polynucleotides by ligation of multiple oligomers
US09/241,353 US6001614A (en) 1998-07-24 1999-02-02 Methods of synthesizing labeled polynucleotides by ligation of multiple oligomers
US09/241,979 US6020138A (en) 1998-07-24 1999-02-02 Methods for detecting nucleic acids by ligation of multiple oligomers
US09/245,984 US6013456A (en) 1998-07-24 1999-02-05 Methods of sequencing polynucleotides by ligation of multiple oligomers
PCT/US1999/014160 WO2000005412A1 (fr) 1998-07-24 1999-07-22 Synthetisation de polynucleotides par ligature d'oligomeres multiples

Publications (1)

Publication Number Publication Date
DK1017855T3 true DK1017855T3 (da) 2005-03-14

Family

ID=27494390

Family Applications (1)

Application Number Title Priority Date Filing Date
DK99937170T DK1017855T3 (da) 1998-07-24 1999-07-22 Fremgangsmåde til syntetisering af polynucleotider ved ligering af flere oligomerer

Country Status (10)

Country Link
EP (1) EP1017855B1 (fr)
JP (1) JP2002521036A (fr)
AT (1) ATE281532T1 (fr)
AU (1) AU772995B2 (fr)
CA (1) CA2304258C (fr)
DE (1) DE69921594T2 (fr)
DK (1) DK1017855T3 (fr)
ES (1) ES2233062T3 (fr)
IL (1) IL134729A (fr)
WO (1) WO2000005412A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1530581A4 (fr) * 2002-06-12 2006-08-30 Datascope Investment Corp Molecules de marquage polymeres
US7361821B2 (en) * 2002-09-20 2008-04-22 Intel Corporation Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading
AU2003278852A1 (en) * 2002-09-20 2004-05-13 Intel Corporation Controlled alignment of nano-barcodes encoding specific information for scanning probe microscopy (spm) reading
AU2003283575A1 (en) * 2002-11-14 2004-06-03 John Nicholas Housby Polymorphism assay
JP2007275006A (ja) * 2006-04-10 2007-10-25 Hitachi High-Technologies Corp 核酸検出用プローブ作製法
GB201612011D0 (en) 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
WO2018188856A1 (fr) * 2017-04-13 2018-10-18 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Marquage de sondes oligonucléotidiques par ligature à voies multiples
CN115852495B (zh) * 2022-12-30 2023-09-15 苏州泓迅生物科技股份有限公司 一种基因突变文库的合成方法及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4883750A (en) * 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US5114839A (en) * 1988-05-24 1992-05-19 Gesellschaft Fur Biotechnologische Forsching Mbh Process for dna sequencing using oligonucleotide bank
EP0379559B1 (fr) * 1988-06-24 1996-10-23 Amgen Inc. Procede et reactifs de detection de sequences d'acides nucleiques
US5132215A (en) * 1988-09-15 1992-07-21 Eastman Kodak Company Method of making double-stranded dna sequences
WO1993020096A1 (fr) * 1991-05-08 1993-10-14 Stratagene Bibliotheques d'oligonucleotides utiles pour la production d'amorces
US5681943A (en) * 1993-04-12 1997-10-28 Northwestern University Method for covalently linking adjacent oligonucleotides
DE4336911C2 (de) * 1993-10-28 2002-11-21 Biotechnolog Forschung Gmbh Verfahren zur Primer-Herstellung und Template-Bank dafür
US5888731A (en) * 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction

Also Published As

Publication number Publication date
JP2002521036A (ja) 2002-07-16
CA2304258C (fr) 2010-04-13
EP1017855B1 (fr) 2004-11-03
WO2000005412A1 (fr) 2000-02-03
DE69921594T2 (de) 2005-11-10
ES2233062T3 (es) 2005-06-01
IL134729A0 (en) 2001-04-30
IL134729A (en) 2005-08-31
CA2304258A1 (fr) 2000-02-03
DE69921594D1 (de) 2004-12-09
ATE281532T1 (de) 2004-11-15
AU5205299A (en) 2000-02-14
EP1017855A1 (fr) 2000-07-12
AU772995B2 (en) 2004-05-13

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