DK1069131T3 - Fremgangsmåde til at skille partikelformige substrater fra en oplösning med et minimalt tab af partikler - Google Patents

Fremgangsmåde til at skille partikelformige substrater fra en oplösning med et minimalt tab af partikler

Info

Publication number
DK1069131T3
DK1069131T3 DK00115185T DK00115185T DK1069131T3 DK 1069131 T3 DK1069131 T3 DK 1069131T3 DK 00115185 T DK00115185 T DK 00115185T DK 00115185 T DK00115185 T DK 00115185T DK 1069131 T3 DK1069131 T3 DK 1069131T3
Authority
DK
Denmark
Prior art keywords
solution
loss
detergent
well
beads
Prior art date
Application number
DK00115185T
Other languages
Danish (da)
English (en)
Inventor
Roland Fabis
Kerstin Steinert
Melanie Emmerlich
Helge Lubenow
Joachim Ribbe
Original Assignee
Qiagen Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Gmbh filed Critical Qiagen Gmbh
Application granted granted Critical
Publication of DK1069131T3 publication Critical patent/DK1069131T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Separation Of Solids By Using Liquids Or Pneumatic Power (AREA)
  • Compounds Of Iron (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
DK00115185T 1999-07-15 2000-07-13 Fremgangsmåde til at skille partikelformige substrater fra en oplösning med et minimalt tab af partikler DK1069131T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US35340799A 1999-07-15 1999-07-15

Publications (1)

Publication Number Publication Date
DK1069131T3 true DK1069131T3 (da) 2006-06-26

Family

ID=23388970

Family Applications (1)

Application Number Title Priority Date Filing Date
DK00115185T DK1069131T3 (da) 1999-07-15 2000-07-13 Fremgangsmåde til at skille partikelformige substrater fra en oplösning med et minimalt tab af partikler

Country Status (6)

Country Link
US (2) US6723510B2 (fr)
EP (1) EP1069131B1 (fr)
AT (1) ATE320443T1 (fr)
DE (1) DE60026641T2 (fr)
DK (1) DK1069131T3 (fr)
ES (1) ES2258429T3 (fr)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6849186B2 (en) * 2001-05-02 2005-02-01 Phillips Plastic Corporation Composite particles
CN1617938A (zh) 2002-01-16 2005-05-18 戴诺生物技术有限公司 从单个样品中分离核酸和蛋白质的方法
EP1335003A3 (fr) * 2002-02-06 2004-04-07 Toyo Boseki Kabushiki Kaisha Support magnetique capable de fixer des protéines et methode de purification de protéines utilisant ledit support magnétique
GB0215185D0 (en) 2002-07-01 2002-08-07 Genovision As Binding a target substance
KR100859403B1 (ko) * 2003-03-14 2008-09-22 자이단호진 기타큐슈산교가쿠쥬쓰스이신키코 생체 물질 고정화 칩 및 그의 용도
AU2004236740A1 (en) * 2003-05-02 2004-11-18 Sigma-Aldrich Co. Solid phase cell lysis and capture platform
EP2216083A3 (fr) * 2003-06-27 2010-11-24 Invitrogen Dynal AS Conjuges de particules magnetiques polymeriques et acide aspartique carboxymethyle
AU2004203649B2 (en) 2003-08-12 2006-01-12 F. Hoffmann-La Roche Ag Thermostable Taq polymerase fragment
EP1510577A1 (fr) * 2003-08-29 2005-03-02 Qiagen GmbH Méthode pour l'isolation des acides nucléiques en utilisant des particules magnétiques
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US20120122102A1 (en) 2003-09-11 2012-05-17 Rangarajan Sampath Compositions for use in identification of bacteria
US8546082B2 (en) * 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US7115397B2 (en) * 2003-09-12 2006-10-03 Promega Corporation Methods and kits for purifying his-tagged proteins
US8163895B2 (en) * 2003-12-05 2012-04-24 Ibis Biosciences, Inc. Compositions for use in identification of orthopoxviruses
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
WO2006026248A1 (fr) * 2004-08-25 2006-03-09 Sigma-Aldrich Co. Compositions et procedes faisant appel a des combinaisons de detergents zwitterioniques
WO2007050017A1 (fr) * 2005-10-28 2007-05-03 Ge Healthcare Bio-Sciences Ab Support de séparation ayant différentes fonctionnalités
ATE517192T1 (de) * 2006-08-10 2011-08-15 Merck Patent Gmbh Verfahren zur isolierung von zellen
EP2167965B1 (fr) * 2007-06-22 2013-04-10 B.R.A.H.M.S GmbH Procédé de détection d'analytes
DE102008030142A1 (de) * 2008-06-27 2009-12-31 Qiagen Gmbh Verfahren zur Proteinaufreinigung unter denaturierenden Bedingungen
TWI369494B (en) * 2008-12-12 2012-08-01 Univ Nat Taiwan Compact disk based platform for separating and detecting immunomagnetic bead labeled cells
TWI373617B (en) * 2009-03-03 2012-10-01 Univ Nat Taiwan Compact disk based system for separating immunomagnetic bead labeled particulates and method thereof
TWI381163B (zh) * 2009-09-10 2013-01-01 Univ Nat Taiwan 正篩選免疫磁珠標定微粒之分離方法
US8932809B2 (en) 2010-01-19 2015-01-13 Opgen, Inc. Methods and kits for isolating nucleic acid from an organism
CN103703143B (zh) 2011-01-31 2016-12-14 爱普瑞斯生物公司 鉴定细胞中的多个表位的方法
US11560585B2 (en) 2011-01-31 2023-01-24 Roche Sequencing Solutions, Inc. Methods of identifying multiple epitopes in cells
WO2013024763A1 (fr) * 2011-08-12 2013-02-21 独立行政法人理化学研究所 Procédé pour la production d'un aptamère d'acide nucléique
KR101933621B1 (ko) 2012-09-28 2018-12-28 삼성전자주식회사 소포를 분리하기 위한 조성물, 키트 및 이를 이용하여 소포를 분리하는 방법
JP2016514455A (ja) 2013-03-15 2016-05-23 フリューダイム・コーポレイション 単一細胞における標的タンパク質および標的核酸の同時検出
US20150132743A1 (en) * 2013-09-04 2015-05-14 Fluidigm Corporation Proximity assays for detecting nucleic acids and proteins in a single cell
WO2015166415A1 (fr) * 2014-04-28 2015-11-05 Universidade De Aveiro Nanoparticules chélatrices de silice magnétique modifiée, leur utilisation et leur préparation
US9663780B2 (en) 2014-10-15 2017-05-30 Alpaqua Engineering, LLC Solid-core ring-magnet
CN105505900B (zh) * 2016-02-01 2019-10-11 广西民族大学 一种巯基蛋白酶的提取方法
JP6690327B2 (ja) * 2016-03-14 2020-04-28 富士レビオ株式会社 尿中プロスタグランジンｅ主要代謝物の測定方法及びキット
MA53439A (fr) 2018-08-23 2021-06-30 Alpaqua Eng Llc Aimant à noyau solide
US11242519B2 (en) 2018-08-23 2022-02-08 Alpaqua Engineering, LLC Discontinuous wall hollow core magnet
US12372497B2 (en) 2021-08-18 2025-07-29 Alpaqua Engineering, LLC Plate cushion device having a compression gap lock
CN115792212B (zh) * 2022-10-26 2025-02-11 深圳市瑞图生物技术有限公司 一种磁珠磁损率的测定方法

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2385959A (en) * 1941-06-13 1945-10-02 Frank B Yingling Valve operating mechanism
US4009213A (en) * 1971-11-11 1977-02-22 Henkel & Cie G.M.B.H. Continuous process for the separation of mixtures of fatty substances of different melting points
CA1304886C (fr) 1986-07-10 1992-07-07 Heinz Dobeli Resines chelatant des metaux
US5047513A (en) 1986-07-10 1991-09-10 Hoffmann-La Roche Inc. Metal chelate resins
CA1340522C (fr) * 1987-03-10 1999-05-04 Heinz Dobeli Proteins hybrides renfermant des histidines voisines pour une purification amelioree
US4888367A (en) * 1987-12-01 1989-12-19 The Standard Oil Company Process for neutralizing crosslinked poly (acrylic acid)
JPH01180451A (ja) * 1988-01-11 1989-07-18 Showa Denko Kk アフィニティー分離用吸着体
US5147529A (en) 1988-08-10 1992-09-15 E. I. Du Pont De Nemours And Company Method for automatically processing magnetic solid phase reagents
CA2025178A1 (fr) * 1989-10-02 1991-04-03 William G. Weisburg Sondes d'acide nucleique pour la detection des spirochetes de la maladie de lyme
US5128103A (en) 1990-12-14 1992-07-07 E. I. Du Pont De Nemours And Company Apparatus for automatically processing magnetic solid phase reagents
US5439829A (en) 1991-01-30 1995-08-08 Eli Lilly And Company Immobilization of biologically active molecules by changing the Oxidation state of a chelated transition metal ion
US5646016A (en) 1991-02-06 1997-07-08 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules
US5385959A (en) * 1992-04-29 1995-01-31 Lever Brothers Company, Division Of Conopco, Inc. Capsule which comprises a component subject to degradation and a composite polymer
ATE195081T1 (de) 1992-09-24 2000-08-15 Amersham Pharm Biotech Uk Ltd Verfahren und vorrichtung zur magnetischen abscheidung
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
DE4423878A1 (de) 1994-07-07 1996-01-11 Boehringer Mannheim Gmbh Vorrichtung und Verfahren zum Abscheiden von magnetischen Mikropartikeln
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
DE19512368A1 (de) 1995-04-01 1996-10-02 Boehringer Mannheim Gmbh System zur Freisetzung und Isolierung von Nukleinsäuren
US5798442A (en) * 1995-04-21 1998-08-25 Merck Frosst Canada, Inc. Peptidyl derivatives as inhibitors of pro-apoptotic cysteine proteinases
US6100079A (en) 1996-02-25 2000-08-08 Precision System Science Co., Ltd. Method for treating biopolymers, microorganisms or materials by using more than one type of magnetic particles
AU738336B2 (en) 1996-05-20 2001-09-13 Precision System Science Co., Ltd. Control method and apparatus for controlling magnetic particles by a sample distributor
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
JP3275032B2 (ja) * 1997-03-03 2002-04-15 独立行政法人産業技術総合研究所 環境浄化材料及びその製造方法
US6348318B1 (en) * 1997-04-04 2002-02-19 Biosite Diagnostics Methods for concentrating ligands using magnetic particles
FI990082A0 (fi) * 1999-01-18 1999-01-18 Labsystems Oy Puhdistusprosessi magneettipartikkeleita käyttäen

Also Published As

Publication number Publication date
DE60026641D1 (de) 2006-05-11
ES2258429T3 (es) 2006-09-01
US20040171077A1 (en) 2004-09-02
US6723510B2 (en) 2004-04-20
US20010014466A1 (en) 2001-08-16
ATE320443T1 (de) 2006-04-15
EP1069131A1 (fr) 2001-01-17
EP1069131B1 (fr) 2006-03-15
DE60026641T2 (de) 2007-01-25

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